Rorug05G0422700

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000474
Physical Location & Seq
Reverse (-)
58734794 .. 58736494
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0422700.1

Sequence Viewer

Length: 420 bp
ATGGGGACTCAATTAAATGAGATAAGAGAGTCCCCGCATCCGCTCCCGACCTTCTTACACAGAACCCCCAAAACTCACAGGACTCTGGAAGCAAGGGCTTCAGCAACGCTCAATCCCTTTCGCGCGCTTAAGCTTGGTTCAATTTCTTTTCTCTCTCATGCTCCTTTTGCTTTGATTCTGATTCCCTCTTCCCTTCGACCCCGTCGAGCTTCTGGTGTATCTACTGAGTCTGCTACAGGTGCAGTTGAGATTGCTGGGAAACATTTTCCAAGAGTAGAATGCAAATGGGGTGATCAGGGTCTTGAGGAAATTATTGCAGATAGTTCAATTCTTGGTGTTGCTGTGGTTTTAGCTGGCCAAGCTCAGGTTGATTTCTCACTGAAGCTTCTCAAAGCAGGGAAGAATGTCCTTCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

139

Amino Acids

14.92

Weight (kDa)

9.89

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 43
AccII CGCG 2 cut(s) 123, 125
AciI CCGC 2 cut(s) 35, 41
AcoI YGGCCR 1 cut(s) 355
AcuI CTGAAG 2 cut(s) 84, 401
AfiI CCNNNNNNNGG 1 cut(s) 364
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 3 cut(s) 141, 327, 413
AluBI AGCT 5 cut(s) 133, 209, 353, 362, 385
AluI AGCT 5 cut(s) 133, 209, 353, 362, 385
AoxI GGCC 1 cut(s) 355
AspLEI GCGC 2 cut(s) 125, 127
AsuHPI GGTGA 1 cut(s) 302
BalI TGGCCA 1 cut(s) 357
BclI TGATCA 1 cut(s) 292
BfmI CTRYAG 1 cut(s) 234
BfrI CTTAAG 1 cut(s) 128
BmsI GCATC 1 cut(s) 46
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMII CTCAG 2 cut(s) 216, 377
BsePI GCGCGC 1 cut(s) 123
BseYI CCCAGC 1 cut(s) 254
BsgI GTGCAG 1 cut(s) 261
Bsh1236I CGCG 2 cut(s) 123, 125
BshFI GGCC 1 cut(s) 357
BslFI GGGAC 2 cut(s) 16, 19
BslI CCNNNNNNNGG 1 cut(s) 364
BsmFI GGGAC 2 cut(s) 16, 19
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 1 cut(s) 357
Bsp143I GATC 1 cut(s) 292
BspACI CCGC 2 cut(s) 35, 41
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 2 cut(s) 217, 376
BspFNI CGCG 2 cut(s) 123, 125
BspTI CTTAAG 1 cut(s) 128
BsrBI CCGCTC 1 cut(s) 43
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 193
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 2 cut(s) 125, 355
BstDEI CTNAG 2 cut(s) 225, 363
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 2 cut(s) 123, 125
BstHHI GCGC 2 cut(s) 125, 127
BstKTI GATC 1 cut(s) 295
BstMBI GATC 1 cut(s) 292
BstMWI GCNNNNNNNGC 3 cut(s) 167, 239, 359
BstSFI CTRYAG 1 cut(s) 234
BstUI CGCG 2 cut(s) 123, 125
BsuRI GGCC 1 cut(s) 357
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 125, 355
CfoI GCGC 2 cut(s) 125, 127
CviAII CATG 1 cut(s) 158
CviJI RGCY 7 cut(s) 98, 133, 209, 353, 357, 362, 385
CviKI_1 RGCY 7 cut(s) 98, 133, 209, 353, 357, 362, 385
DdeI CTNAG 2 cut(s) 225, 363
DpnI GATC 1 cut(s) 294
DpnII GATC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 355
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 84, 401
FaeI CATG 1 cut(s) 161
FaiI YATR 1 cut(s) 159
FaqI GGGAC 2 cut(s) 16, 19
FatI CATG 1 cut(s) 157
FauI CCCGC 1 cut(s) 42
FbaI TGATCA 1 cut(s) 292
FokI GGATG 1 cut(s) 24
GlaI GCGC 2 cut(s) 124, 126
GsaI CCCAGC 1 cut(s) 258
HaeIII GGCC 1 cut(s) 357
HhaI GCGC 2 cut(s) 125, 127
Hin1II CATG 1 cut(s) 161
Hin6I GCGC 2 cut(s) 123, 125
HinP1I GCGC 2 cut(s) 123, 125
HindIII AAGCTT 2 cut(s) 131, 383
HinfI GANTC 6 cut(s) 7, 29, 82, 175, 181, 227
HphI GGTGA 1 cut(s) 302
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 3 cut(s) 46, 86, 302
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 3 cut(s) 61, 203, 419
HpyCH4V TGCA 3 cut(s) 242, 282, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 239, 359
HpyF3I CTNAG 2 cut(s) 225, 363
Hsp92II CATG 1 cut(s) 161
HspAI GCGC 2 cut(s) 123, 125
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 1 cut(s) 292
LmnI GCTCC 2 cut(s) 48, 166
LpnPI CCDG 9 cut(s) 64, 71, 198, 222, 240, 281, 339, 350, 381
LweI GCATC 1 cut(s) 46
MalI GATC 1 cut(s) 294
MbiI CCGCTC 1 cut(s) 43
MboI GATC 1 cut(s) 292
MboII GAAGA 2 cut(s) 180, 412
MlsI TGGCCA 1 cut(s) 357
MluCI AATT 4 cut(s) 11, 141, 309, 327
MluNI TGGCCA 1 cut(s) 357
MlyI GAGTC 3 cut(s) 38, 76, 236
MnlI CCTC 2 cut(s) 196, 298
Mox20I TGGCCA 1 cut(s) 357
MscI TGGCCA 1 cut(s) 357
MseI TTAA 2 cut(s) 14, 129
Msp20I TGGCCA 1 cut(s) 357
MspCI CTTAAG 1 cut(s) 128
Mva1269I GAATGC 1 cut(s) 284
MvnI CGCG 2 cut(s) 123, 125
MwoI GCNNNNNNNGC 3 cut(s) 167, 239, 359
NdeII GATC 1 cut(s) 292
NlaIII CATG 1 cut(s) 161
PauI GCGCGC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 175, 181
PflFI GACNNNGTC 1 cut(s) 201
PleI GAGTC 3 cut(s) 37, 76, 235
PpsI GAGTC 3 cut(s) 37, 76, 235
PspFI CCCAGC 1 cut(s) 254
PsyI GACNNNGTC 1 cut(s) 201
PteI GCGCGC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 14, 129
Sau3AI GATC 1 cut(s) 292
SchI GAGTC 3 cut(s) 38, 76, 236
SetI ASST 9 cut(s) 53, 135, 211, 241, 355, 364, 369, 387, 418
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 234
SmlI CTYRAG 2 cut(s) 128, 302
SmoI CTYRAG 2 cut(s) 128, 302
Sse9I AATT 4 cut(s) 11, 141, 309, 327
SsiI CCGC 2 cut(s) 35, 41
TaqI TCGA 2 cut(s) 196, 205
TasI AATT 4 cut(s) 11, 141, 309, 327
TfiI GAWTC 2 cut(s) 175, 181
Tru1I TTAA 2 cut(s) 14, 129
Tru9I TTAA 2 cut(s) 14, 129
TscAI CASTG 1 cut(s) 384
TspRI CASTG 1 cut(s) 384
Tth111I GACNNNGTC 1 cut(s) 201
Vha464I CTTAAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.