Rorug05G0423600

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000474
Physical Location & Seq
Forward (+)
58914090 .. 58916768
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0423600.1

Sequence Viewer

Length: 486 bp
ATGGATCTTTGTCGTAAGCGTGCTAGAGAAGAAGAGGCGGAGGAGGATGAAGAAAGGATAGGGAAAATGAAAGCCTTTTTGGCTGATGTAACTGGATTTATAGCAGCAATAGTAGCTTGGTACCATTGTAAGTTACTCACAAGAAGAGAGCCCTTGCCTGATTGGGATGAACATAGACGATTGTATTTGAATCGATTAAGTGGATCTGAAGTTGAGTGTATGAAAAACTTAAGAGTCAGCATGTATGGATTTTCAAAACTTTGCCAAGTCTTATATGACAAAGGTGGATTACGAAGAACAAGGCATGTATCTATTGAAGAATCTGTATTTTGCGAAATCAACGAAGATGAGATTAGAACTGTTGAAGCCACTAATGAGTGGACTGTATTTCATGATCAATTCGTGATGGATATGTTTCTTGACTATCAAAGGCATCGACCTCAACAAGCGCAGCAAGAACAAGGAGACCTTGCAAATGTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

161

Amino Acids

19.1

Weight (kDa)

5.31

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 66 - 110 4.4e-08 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 120
AciI CCGC 1 cut(s) 38
AclWI GGATC 2 cut(s) 12, 211
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 1 cut(s) 122
AflII CTTAAG 1 cut(s) 229
AgsI TTSAA 4 cut(s) 190, 255, 317, 365
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 2 cut(s) 12, 211
ApeKI GCWGC 2 cut(s) 104, 451
Asp718I GGTACC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 451
BaeI ACNNNNGTAYC 2 cut(s) 112, 145
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 153
BbvI GCAGC 2 cut(s) 116, 463
BccI CCATC 1 cut(s) 400
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 1 cut(s) 24
BfrI CTTAAG 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 2 cut(s) 105, 452
BlsI GCNGC 2 cut(s) 106, 453
BmiI GGNNCC 1 cut(s) 122
BmsI GCATC 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 459
Bse1I ACTGG 1 cut(s) 97
BseCI ATCGAT 1 cut(s) 193
BseGI GGATG 2 cut(s) 52, 172
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 2 cut(s) 116, 463
BshNI GGYRCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 459
Bso31I GGTCTC 1 cut(s) 459
Bsp1286I GDGCHC 1 cut(s) 153
Bsp143I GATC 3 cut(s) 4, 203, 394
BspACI CCGC 1 cut(s) 38
BspDI ATCGAT 1 cut(s) 193
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 122
BspPI GGATC 2 cut(s) 12, 211
BspT107I GGYRCC 1 cut(s) 120
BspTI CTTAAG 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 459
BsrI ACTGG 1 cut(s) 97
BssMI GATC 3 cut(s) 4, 203, 394
Bst4CI ACNGT 2 cut(s) 361, 385
Bst6I CTCTTC 2 cut(s) 27, 139
BstAFI CTTAAG 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 21
BstF5I GGATG 2 cut(s) 52, 172
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 3 cut(s) 7, 206, 397
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 3 cut(s) 4, 203, 394
BstMWI GCNNNNNNNGC 2 cut(s) 80, 113
BstNSI RCATGY 2 cut(s) 244, 308
BstV1I GCAGC 2 cut(s) 116, 463
BstX2I RGATCY 2 cut(s) 4, 203
BstYI RGATCY 2 cut(s) 4, 203
Bsu15I ATCGAT 1 cut(s) 193
BsuTUI ATCGAT 1 cut(s) 193
BtsCI GGATG 2 cut(s) 52, 172
Cac8I GCNNGC 1 cut(s) 21
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 451
ClaI ATCGAT 1 cut(s) 193
Csp6I GTAC 1 cut(s) 121
CviAII CATG 3 cut(s) 241, 305, 392
CviJI RGCY 5 cut(s) 74, 83, 116, 151, 368
CviKI_1 RGCY 5 cut(s) 74, 83, 116, 151, 368
CviQI GTAC 1 cut(s) 121
DpnI GATC 3 cut(s) 6, 205, 396
DpnII GATC 3 cut(s) 4, 203, 394
Eam1104I CTCTTC 2 cut(s) 27, 139
EarI CTCTTC 2 cut(s) 27, 139
EciI GGCGGA 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 153
FaeI CATG 3 cut(s) 244, 308, 395
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 3 cut(s) 240, 304, 391
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 2 cut(s) 105, 452
FokI GGATG 2 cut(s) 59, 179
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 2 cut(s) 105, 452
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 450
GluI GCNGC 2 cut(s) 105, 452
HhaI GCGC 1 cut(s) 451
Hin1II CATG 3 cut(s) 244, 308, 395
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HinfI GANTC 3 cut(s) 190, 234, 320
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 3 cut(s) 392, 403, 419
Hpy8I GTNNAC 1 cut(s) 381
HpyCH4III ACNGT 2 cut(s) 361, 385
HpyCH4V TGCA 1 cut(s) 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 113
Hsp92II CATG 3 cut(s) 244, 308, 395
HspAI GCGC 1 cut(s) 449
KpnI GGTACC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 3 cut(s) 4, 203, 394
LpnPI CCDG 2 cut(s) 78, 171
Lsp1109I GCAGC 2 cut(s) 116, 463
LweI GCATC 1 cut(s) 442
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 88, 132
MalI GATC 3 cut(s) 6, 205, 396
MboI GATC 3 cut(s) 4, 203, 394
MboII GAAGA 7 cut(s) 41, 44, 62, 156, 306, 329, 356
MflI RGATCY 2 cut(s) 4, 203
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 1 cut(s) 398
MlyI GAGTC 1 cut(s) 243
MnlI CCTC 4 cut(s) 28, 34, 37, 450
MseI TTAA 2 cut(s) 197, 230
MspCI CTTAAG 1 cut(s) 229
MwoI GCNNNNNNNGC 2 cut(s) 80, 113
NdeII GATC 3 cut(s) 4, 203, 394
NlaIII CATG 3 cut(s) 244, 308, 395
NlaIV GGNNCC 1 cut(s) 122
NspI RCATGY 2 cut(s) 244, 308
PagI TCATGA 1 cut(s) 391
PfeI GAWTC 2 cut(s) 190, 320
PkrI GCNGC 2 cut(s) 106, 453
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 122
PsuI RGATCY 2 cut(s) 4, 203
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SaqAI TTAA 2 cut(s) 197, 230
SatI GCNGC 2 cut(s) 105, 452
Sau3AI GATC 3 cut(s) 4, 203, 394
SchI GAGTC 1 cut(s) 243
SduI GDGCHC 1 cut(s) 153
SetI ASST 4 cut(s) 118, 286, 442, 471
SfaNI GCATC 1 cut(s) 442
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 1 cut(s) 398
SsiI CCGC 1 cut(s) 38
SspMI CTAG 1 cut(s) 24
TaaI ACNGT 2 cut(s) 361, 385
TaqI TCGA 2 cut(s) 193, 436
TasI AATT 1 cut(s) 398
TfiI GAWTC 2 cut(s) 190, 320
Tru1I TTAA 2 cut(s) 197, 230
Tru9I TTAA 2 cut(s) 197, 230
TseI GCWGC 2 cut(s) 104, 451
TspDTI ATGAA 5 cut(s) 63, 83, 183, 236, 380
Vha464I CTTAAG 1 cut(s) 229
XceI RCATGY 2 cut(s) 244, 308
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.