Rh5CG524700

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000474
Physical Location & Seq
Forward (+)
73494354 .. 73494767
414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG524700.1

Sequence Viewer

Length: 414 bp
ATGTCATCTGCAGCAGCTCAAGTTCCTGATTATGACTATCTACCAGATGAGTCCATTACCCCTGCTAGCTCTGAAGATGATGAAGCCTACGCTCCAGTTACGATCGAGTATGGGTTTCCACCAGAGCCGTTCCGTGGATATTATGACAATTTAGACCAATGTGAAGACAACTTCAACCTAAATTGTGTTAAAGAGATTTACCGCAGCATGTTCAAAACCGAGCCTCTTAATAAGAACTGTTGCAAGAACCTTGTGCAGATGGGATATGGCTGTCACAGTAGGTTAGCGAAATTTGCGAACCCGAGAAACAAAGAAAAAGCTTCCATGGCATTGGCTAAGAGTGTGCAGATTTGGCAAAAGTGTGTCTCAGTTGTTGAAACTGGTCGATTCCTTGCTGTTCCTGCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

137

Amino Acids

15.44

Weight (kDa)

4.99

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 53 - 122 3.7e-11 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 202
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 93
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 3 cut(s) 175, 214, 377
AluBI AGCT 3 cut(s) 17, 69, 320
AluI AGCT 3 cut(s) 17, 69, 320
Alw26I GTCTC 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 301
ApeKI GCWGC 3 cut(s) 11, 14, 204
ApoI RAATTY 1 cut(s) 290
AsuNHI GCTAGC 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 301
BbsI GAAGAC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 23, 26, 216
BccI CCATC 1 cut(s) 253
BceAI ACGGC 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 66
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 3 cut(s) 12, 15, 205
BlsI GCNGC 3 cut(s) 13, 16, 206
BmeT110I CYCGRG 1 cut(s) 301
BmtI GCTAGC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 133, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 95, 385
BseDI CCNNGG 2 cut(s) 133, 324
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 95, 385
BseXI GCAGC 3 cut(s) 23, 26, 216
BsgI GTGCAG 2 cut(s) 275, 365
Bsh1285I CGRYCG 1 cut(s) 105
BsiEI CGRYCG 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 301
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 370
BsoBI CYCGRG 1 cut(s) 301
Bsp143I GATC 1 cut(s) 102
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 1 cut(s) 202
BspCNI CTCAG 1 cut(s) 380
BspMAI CTGCAG 1 cut(s) 13
BspOI GCTAGC 1 cut(s) 69
BsrI ACTGG 2 cut(s) 95, 385
BssECI CCNNGG 2 cut(s) 133, 324
BssMI GATC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 239, 278
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 336, 367
BstDSI CCRYGG 2 cut(s) 133, 324
BstKTI GATC 1 cut(s) 105
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 1 cut(s) 102
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 4 cut(s) 293, 326, 352, 401
BstNSI RCATGY 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 3 cut(s) 23, 26, 216
BstV2I GAAGAC 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 331
BtgI CCRYGG 2 cut(s) 133, 324
Cac8I GCNNGC 1 cut(s) 67
CviAII CATG 2 cut(s) 208, 325
CviJI RGCY 8 cut(s) 17, 69, 86, 127, 223, 270, 320, 335
CviKI_1 RGCY 8 cut(s) 17, 69, 86, 127, 223, 270, 320, 335
DdeI CTNAG 2 cut(s) 336, 367
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 2 cut(s) 211, 328
FaiI YATR 6 cut(s) 33, 111, 144, 209, 267, 326
FatI CATG 2 cut(s) 207, 324
Fnu4HI GCNGC 3 cut(s) 12, 15, 205
Fsp4HI GCNGC 3 cut(s) 12, 15, 205
FspBI CTAG 1 cut(s) 66
GluI GCNGC 3 cut(s) 12, 15, 205
GsuI CTGGAG 1 cut(s) 78
Hin1II CATG 2 cut(s) 211, 328
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 2 cut(s) 50, 387
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 26
HpyCH4III ACNGT 2 cut(s) 239, 278
HpyCH4V TGCA 4 cut(s) 11, 243, 256, 346
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 326, 352, 401
HpyF3I CTNAG 2 cut(s) 336, 367
Hsp92II CATG 2 cut(s) 211, 328
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 97, 409
LpnPI CCDG 6 cut(s) 39, 57, 75, 108, 135, 366
Lsp1109I GCAGC 3 cut(s) 23, 26, 216
MaeI CTAG 1 cut(s) 66
MaeIII GTNAC 2 cut(s) 97, 272
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 2 cut(s) 86, 176
MluCI AATT 3 cut(s) 148, 181, 290
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 1 cut(s) 234
MseI TTAA 2 cut(s) 189, 228
MwoI GCNNNNNNNGC 4 cut(s) 293, 326, 352, 401
NcoI CCATGG 1 cut(s) 324
NdeII GATC 1 cut(s) 102
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 2 cut(s) 211, 328
NmuCI GTSAC 1 cut(s) 272
NspI RCATGY 1 cut(s) 211
PfeI GAWTC 1 cut(s) 387
PkrI GCNGC 3 cut(s) 13, 16, 206
Ple19I CGATCG 1 cut(s) 105
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PstI CTGCAG 1 cut(s) 13
PvuI CGATCG 1 cut(s) 105
SaqAI TTAA 2 cut(s) 189, 228
SatI GCNGC 3 cut(s) 12, 15, 205
Sau3AI GATC 1 cut(s) 102
SchI GAGTC 1 cut(s) 59
SetI ASST 6 cut(s) 19, 71, 180, 252, 284, 322
SfcI CTRYAG 1 cut(s) 9
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 3 cut(s) 148, 181, 290
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 239, 278
TaqI TCGA 2 cut(s) 105, 385
TasI AATT 3 cut(s) 148, 181, 290
TfiI GAWTC 1 cut(s) 387
Tru1I TTAA 2 cut(s) 189, 228
Tru9I TTAA 2 cut(s) 189, 228
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 3 cut(s) 11, 14, 204
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 1 cut(s) 122
XapI RAATTY 1 cut(s) 290
XceI RCATGY 1 cut(s) 211
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.