Rh2DG385300

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000474
Physical Location & Seq
Reverse (-)
55756356 .. 55756838
483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG385300.1

Sequence Viewer

Length: 483 bp
ATGGCAAGAGTTGATCAAAATGTGTTTGTCAAGGTGAGTTTGGTAGCACTACTCATGTCGTTTGCAGCAGCTCAAGACTTTGTTGCTTATGAAGTCTATAATGATGCCACAAGCCCTATAAGCGTTGAGGTTGAAGTCATTGATGCCTACCCTCCAATTTCTCCAGAAGACCAGGATGCTCTCCCTCCAGAACCATCACCGGGAGCTTACAAGAAGTTGAAGAATTGTGCAAACAAGATTACAGCAGATTATGCCGAAGAGATCTACGAAGGTATTTTCGAAGGCGAGCCTCTCACTACAGATTGCTGCAAAGCCCTTGTGCATCTGGGATATGATTGTCACATCCAGTTAGTGAAATTTTTGCTCTCGAGTCCGGAACTTAAAGAAAAAGCTTCCGAGGTATTGCCTAGGAGTGTGCAGCTTTGGAAAACATGTGCCTTGGTGGTTGACGTTGATCAATCTATTGCTCCTTCTCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

160

Amino Acids

17.58

Weight (kDa)

4.43

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 75 - 145 2.4e-11 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 373
AcsI RAATTY 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 200
AflIII ACRYGT 1 cut(s) 431
AgsI TTSAA 2 cut(s) 134, 220
AjnI CCWGG 1 cut(s) 171
AluBI AGCT 4 cut(s) 71, 206, 392, 421
AluI AGCT 4 cut(s) 71, 206, 392, 421
Ama87I CYCGRG 1 cut(s) 367
Aor13HI TCCGGA 1 cut(s) 373
ApeKI GCWGC 4 cut(s) 65, 68, 306, 418
ApoI RAATTY 1 cut(s) 356
AspA2I CCTAGG 1 cut(s) 407
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 46, 189
AsuII TTCGAA 1 cut(s) 279
AvaI CYCGRG 1 cut(s) 367
AvrII CCTAGG 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 4 cut(s) 77, 80, 293, 430
BccI CCATC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 173
BclI TGATCA 2 cut(s) 13, 454
BcnI CCSGG 1 cut(s) 201
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 261
BisI GCNGC 4 cut(s) 66, 69, 307, 419
BlnI CCTAGG 1 cut(s) 407
BlsI GCNGC 4 cut(s) 67, 70, 308, 420
Bme1390I CCNGG 2 cut(s) 173, 201
BmeT110I CYCGRG 1 cut(s) 367
BmrFI CCNGG 2 cut(s) 173, 201
BmsI GCATC 4 cut(s) 94, 133, 166, 331
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 2 cut(s) 147, 171
Bpu14I TTCGAA 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 201
BsaJI CCNNGG 3 cut(s) 396, 407, 438
BsaWI WCCGGW 1 cut(s) 373
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 346
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 1 cut(s) 173
BseDI CCNNGG 3 cut(s) 396, 407, 438
BseGI GGATG 2 cut(s) 181, 342
BseLI CCNNNNNNNGG 1 cut(s) 200
BseNI ACTGG 1 cut(s) 346
BseXI GCAGC 4 cut(s) 77, 80, 293, 430
BsgI GTGCAG 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 2 cut(s) 200, 374
BslI CCNNNNNNNGG 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 279
Bsp13I TCCGGA 1 cut(s) 373
Bsp143I GATC 3 cut(s) 13, 261, 454
BspEI TCCGGA 1 cut(s) 373
BspT104I TTCGAA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 3 cut(s) 396, 407, 438
BssMI GATC 3 cut(s) 13, 261, 454
BssT1I CCWWGG 2 cut(s) 407, 438
Bst2UI CCWGG 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 279
BstC8I GCNNGC 1 cut(s) 287
BstF5I GGATG 2 cut(s) 181, 342
BstKTI GATC 3 cut(s) 16, 264, 457
BstMBI GATC 3 cut(s) 13, 261, 454
BstMWI GCNNNNNNNGC 2 cut(s) 120, 251
BstNI CCWGG 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 435
BstSCI CCNGG 2 cut(s) 171, 199
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 4 cut(s) 77, 80, 293, 430
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BtsCI GGATG 2 cut(s) 181, 342
Cac8I GCNNGC 1 cut(s) 287
CviAII CATG 2 cut(s) 55, 432
CviJI RGCY 7 cut(s) 71, 114, 206, 289, 314, 392, 421
CviKI_1 RGCY 7 cut(s) 71, 114, 206, 289, 314, 392, 421
DpnI GATC 3 cut(s) 15, 263, 456
DpnII GATC 3 cut(s) 13, 261, 454
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 407, 438
Eco88I CYCGRG 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 407, 438
ErhI CCWWGG 2 cut(s) 407, 438
FaeI CATG 2 cut(s) 58, 435
FaiI YATR 7 cut(s) 56, 90, 99, 119, 252, 333, 433
FatI CATG 2 cut(s) 54, 431
FbaI TGATCA 2 cut(s) 13, 454
Fnu4HI GCNGC 4 cut(s) 66, 69, 307, 419
FokI GGATG 2 cut(s) 188, 329
Fsp4HI GCNGC 4 cut(s) 66, 69, 307, 419
FspBI CTAG 1 cut(s) 408
GluI GCNGC 4 cut(s) 66, 69, 307, 419
GsuI CTGGAG 2 cut(s) 147, 171
HapII CCGG 2 cut(s) 200, 374
Hin1II CATG 2 cut(s) 58, 435
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 1 cut(s) 370
HpaII CCGG 2 cut(s) 200, 374
HphI GGTGA 2 cut(s) 46, 189
Hpy166II GTNNAC 1 cut(s) 448
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 5 cut(s) 74, 164, 188, 367, 374
Hpy8I GTNNAC 1 cut(s) 448
HpyAV CCTTC 3 cut(s) 263, 275, 480
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 65, 230, 309, 322, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 251
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 2 cut(s) 58, 435
Kpn2I TCCGGA 1 cut(s) 373
Ksp22I TGATCA 2 cut(s) 13, 454
Kzo9I GATC 3 cut(s) 13, 261, 454
LmnI GCTCC 2 cut(s) 203, 472
LpnPI CCDG 8 cut(s) 158, 177, 185, 201, 213, 311, 359, 387
Lsp1109I GCAGC 4 cut(s) 77, 80, 293, 430
LweI GCATC 4 cut(s) 94, 133, 166, 331
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 338
MalI GATC 3 cut(s) 15, 263, 456
MboI GATC 3 cut(s) 13, 261, 454
MboII GAAGA 3 cut(s) 179, 232, 269
MflI RGATCY 1 cut(s) 261
MluCI AATT 3 cut(s) 156, 223, 356
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 5 cut(s) 121, 162, 195, 300, 391
MroI TCCGGA 1 cut(s) 373
MseI TTAA 2 cut(s) 381, 481
MspI CCGG 2 cut(s) 200, 374
MspR9I CCNGG 2 cut(s) 173, 201
MvaI CCWGG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 120, 251
NciI CCSGG 1 cut(s) 201
NdeII GATC 3 cut(s) 13, 261, 454
NlaIII CATG 2 cut(s) 58, 435
NmuCI GTSAC 1 cut(s) 338
NspI RCATGY 1 cut(s) 435
NspV TTCGAA 1 cut(s) 279
PaeR7I CTCGAG 1 cut(s) 367
PciI ACATGT 1 cut(s) 431
PkrI GCNGC 4 cut(s) 67, 70, 308, 420
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
PscI ACATGT 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 171
PspGI CCWGG 1 cut(s) 171
PsuI RGATCY 1 cut(s) 261
SaqAI TTAA 2 cut(s) 381, 481
SatI GCNGC 4 cut(s) 66, 69, 307, 419
Sau3AI GATC 3 cut(s) 13, 261, 454
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 173, 201
SetI ASST 9 cut(s) 36, 73, 132, 208, 274, 394, 402, 423, 453
SfaNI GCATC 4 cut(s) 94, 133, 166, 331
SfcI CTRYAG 1 cut(s) 297
Sfr274I CTCGAG 1 cut(s) 367
SfuI TTCGAA 1 cut(s) 279
SlaI CTCGAG 1 cut(s) 367
SmlI CTYRAG 2 cut(s) 72, 367
SmoI CTYRAG 2 cut(s) 72, 367
Sse9I AATT 3 cut(s) 156, 223, 356
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 2 cut(s) 171, 199
StyI CCWWGG 2 cut(s) 407, 438
TaiI ACGT 1 cut(s) 453
TaqI TCGA 2 cut(s) 279, 368
TasI AATT 3 cut(s) 156, 223, 356
Tru1I TTAA 2 cut(s) 381, 481
Tru9I TTAA 2 cut(s) 381, 481
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 4 cut(s) 65, 68, 306, 418
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 1 cut(s) 105
XapI RAATTY 1 cut(s) 356
XceI RCATGY 1 cut(s) 435
XhoI CTCGAG 1 cut(s) 367
XmaJI CCTAGG 1 cut(s) 407
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.