AT2G25482

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000474
Physical Location & Seq
Reverse (-)
10845838 .. 10846393
556 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25482.1

Sequence Viewer

Length: 465 bp
ATGGAAAATAAAACCATTTTCTTGGCATTGTTTTCAATCATGGTTTTGCTATCATATTTTAATCCAAGTTTGGCCATAGCAGCCGATCTTGAGGAGATGCTAATCAACGAGTTTGATGTGGTGCTTAAACATTGGCCGAGCCCGGGTGATTATAATCTCAATGTTATCCGTGGACAACCAAGAAAACATCTCAAATATCTTCTTGATTGCGCAGTAAAAATGGGAGCTGGTGGAAATGAATGTAACATTGAAATAAGAGACGTATTTTCTCGGAACAAAAGTTTTTCGAAAGATTGTTGTCGGGTGCTAGTGAAAGGTGGCAGAAAATGCTACACAGAATGGATGAAAATGTTTTTTCAATTCTATCAACTCAACCGTTTTAGTTCTAACGCGATGATCAAAACTAACGAAACATGGAACAAATGTTCTAATGGAACTGAATCTATTTCACCATTTTCTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

154

Amino Acids

17.7

Weight (kDa)

8.74

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 69 - 143 2.2e-13 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 153
Acc16I TGCGCA 1 cut(s) 211
AccB7I CCANNNNNTGG 1 cut(s) 458
AccII CGCG 1 cut(s) 392
AcoI YGGCCR 2 cut(s) 72, 134
AfiI CCNNNNNNNGG 2 cut(s) 143, 458
AgsI TTSAA 3 cut(s) 36, 251, 359
AloI GAACNNNNNNTCC 2 cut(s) 409, 441
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
Alw26I GTCTC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 2 cut(s) 72, 134
ApeKI GCWGC 1 cut(s) 80
AspLEI GCGC 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 143, 144
AsuHPI GGTGA 2 cut(s) 158, 441
AsuII TTCGAA 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 142
BalI TGGCCA 1 cut(s) 74
BanII GRGCYC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 92
BclI TGATCA 1 cut(s) 396
BcnI CCSGG 2 cut(s) 143, 144
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 1 cut(s) 308
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 2 cut(s) 143, 144
BmeT110I CYCGRG 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 143, 144
BmsI GCATC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 2 cut(s) 143, 144
BsaBI GATNNNNATC 2 cut(s) 101, 153
BsaJI CCNNGG 2 cut(s) 142, 169
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 458
Bse8I GATNNNNATC 2 cut(s) 101, 153
BseDI CCNNGG 2 cut(s) 142, 169
BseGI GGATG 1 cut(s) 348
BseJI GATNNNNATC 2 cut(s) 101, 153
BseLI CCNNNNNNNGG 2 cut(s) 143, 458
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 92
Bsh1236I CGCG 1 cut(s) 392
BshFI GGCC 2 cut(s) 74, 136
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 1 cut(s) 143
BslI CCNNNNNNNGG 2 cut(s) 143, 458
BsmAI GTCTC 1 cut(s) 252
BsmBI CGTCTC 1 cut(s) 252
BsnI GGCC 2 cut(s) 74, 136
BsoBI CYCGRG 1 cut(s) 142
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 85, 396
BspANI GGCC 2 cut(s) 74, 136
BspFNI CGCG 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 142, 169
BssMI GATC 2 cut(s) 85, 396
Bst4CI ACNGT 1 cut(s) 377
BstAPI GCANNNNNTGC 1 cut(s) 327
BstBI TTCGAA 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 348
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 2 cut(s) 88, 399
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 2 cut(s) 85, 396
BstMWI GCNNNNNNNGC 2 cut(s) 80, 327
BstSCI CCNGG 2 cut(s) 141, 142
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 92
BstXI CCANNNNNNTGG 1 cut(s) 22
BsuRI GGCC 2 cut(s) 74, 136
BtgI CCRYGG 1 cut(s) 169
BtgZI GCGATG 1 cut(s) 407
BtsCI GGATG 1 cut(s) 348
CfoI GCGC 1 cut(s) 212
Cfr9I CCCGGG 1 cut(s) 142
CviAII CATG 2 cut(s) 40, 414
CviJI RGCY 5 cut(s) 74, 83, 136, 141, 227
CviKI_1 RGCY 5 cut(s) 74, 83, 136, 141, 227
DpnI GATC 2 cut(s) 87, 398
DpnII GATC 2 cut(s) 85, 396
EaeI YGGCCR 2 cut(s) 72, 134
Eco24I GRGCYC 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 143
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 2 cut(s) 43, 417
FaiI YATR 5 cut(s) 41, 55, 77, 153, 415
FatI CATG 2 cut(s) 39, 413
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 355
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 1 cut(s) 308
FspI TGCGCA 1 cut(s) 211
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 81
HaeIII GGCC 2 cut(s) 74, 136
HapII CCGG 1 cut(s) 143
HhaI GCGC 1 cut(s) 212
Hin1II CATG 2 cut(s) 43, 417
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 1 cut(s) 440
HpaII CCGG 1 cut(s) 143
HphI GGTGA 2 cut(s) 158, 441
Hpy166II GTNNAC 1 cut(s) 173
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 89, 203
Hpy8I GTNNAC 1 cut(s) 173
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 327
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 2 cut(s) 43, 417
HspAI GCGC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 2 cut(s) 85, 396
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 3 cut(s) 156, 213, 444
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 1 cut(s) 87
MaeI CTAG 1 cut(s) 308
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 242
MalI GATC 2 cut(s) 87, 398
MboI GATC 2 cut(s) 85, 396
MboII GAAGA 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 143
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 1 cut(s) 359
MluNI TGGCCA 1 cut(s) 74
MnlI CCTC 1 cut(s) 85
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
MseI TTAA 3 cut(s) 60, 126, 463
Msp20I TGGCCA 1 cut(s) 74
MspI CCGG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 143, 144
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 2 cut(s) 80, 327
NciI CCSGG 2 cut(s) 143, 144
NdeII GATC 2 cut(s) 85, 396
NlaIII CATG 2 cut(s) 43, 417
NmeAIII GCCGAG 1 cut(s) 162
NsbI TGCGCA 1 cut(s) 211
NspV TTCGAA 1 cut(s) 287
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 458
PkrI GCNGC 1 cut(s) 82
PsiI TTATAA 1 cut(s) 153
SaqAI TTAA 3 cut(s) 60, 126, 463
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 2 cut(s) 85, 396
ScrFI CCNGG 2 cut(s) 143, 144
SduI GDGCHC 1 cut(s) 143
SetI ASST 3 cut(s) 229, 264, 319
SfaNI GCATC 1 cut(s) 87
SfuI TTCGAA 1 cut(s) 287
SmaI CCCGGG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 1 cut(s) 359
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 2 cut(s) 141, 142
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 287
TasI AATT 1 cut(s) 359
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 3 cut(s) 60, 126, 463
Tru9I TTAA 3 cut(s) 60, 126, 463
TseI GCWGC 1 cut(s) 80
TspDTI ATGAA 2 cut(s) 252, 359
TspGWI ACGGA 1 cut(s) 158
TspMI CCCGGG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 458
XmaI CCCGGG 1 cut(s) 142
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.