RLG00000036378

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000474
Physical Location & Seq
Forward (+)
79793131 .. 79793595
465 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036378

Sequence Viewer

Length: 465 bp
ATGGCAAGATTTGTCATGGTTGGTTTGGTCTTTGCAAGTGTATCATTGCTCATGTCATCTGCAGCAGCTCAAGTTCCTGATTATGACTATCTACCCGATGAGTCCATTAGCCCTGCTAGCTCTGAAGATGATGAAGCCTACGCTCCAGTTACGATCCAGTATGGGTTTCCACCAGAGCCGTTCCGTGGATATTATGACAATTTAGACCAATGTGAAGACAACTTCAACCTAAATTGTGTTAAAGAGATCTACCGCGGTATGTTCAAAACCGAGCCTCTTACTAAGAACTGTTGCAAGAACCTTGTGCAGATGGGATATGGCTGTCACAGTAGGTTAGCGAAATTTGCGAACCCGAGAAATAAAGCAAAAGCTTCCATGGCATTGGCTAAGAGTGTGCAGATTTGGCAAAAGTGCGTATCAGTTGTTGAAACTGGTCGATTCCCTGCTGCTCCTGCTCCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

155

Amino Acids

17.1

Weight (kDa)

5.81

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 70 - 139 5.2e-11 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 255
AciI CCGC 2 cut(s) 253, 255
AclWI GGATC 1 cut(s) 148
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 3 cut(s) 226, 265, 428
AluBI AGCT 3 cut(s) 68, 120, 371
AluI AGCT 3 cut(s) 68, 120, 371
AlwI GGATC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 352
ApeKI GCWGC 3 cut(s) 62, 65, 446
ApoI RAATTY 1 cut(s) 341
AsuNHI GCTAGC 1 cut(s) 116
AvaI CYCGRG 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 74, 77, 433
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 163
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 60
BglII AGATCT 1 cut(s) 246
BisI GCNGC 3 cut(s) 63, 66, 447
BlsI GCNGC 3 cut(s) 64, 67, 448
BmeT110I CYCGRG 1 cut(s) 352
BmtI GCTAGC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 184, 253, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 146, 157, 436
Bse3DI GCAATG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 184, 253, 375
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 44
BseNI ACTGG 3 cut(s) 146, 157, 436
BseXI GCAGC 3 cut(s) 74, 77, 433
BsgI GTGCAG 2 cut(s) 326, 416
Bsh1236I CGCG 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 352
BslI CCNNNNNNNGG 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 352
Bsp143I GATC 2 cut(s) 153, 246
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 2 cut(s) 253, 255
BspFNI CGCG 1 cut(s) 255
BspMAI CTGCAG 1 cut(s) 64
BspOI GCTAGC 1 cut(s) 120
BspPI GGATC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 44
BsrI ACTGG 3 cut(s) 146, 157, 436
BssECI CCNNGG 3 cut(s) 184, 253, 375
BssMI GATC 2 cut(s) 153, 246
BssT1I CCWWGG 1 cut(s) 375
Bst4CI ACNGT 2 cut(s) 290, 329
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 282, 387
BstDSI CCRYGG 3 cut(s) 184, 253, 375
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 2 cut(s) 156, 249
BstMBI GATC 2 cut(s) 153, 246
BstMWI GCNNNNNNNGC 5 cut(s) 117, 344, 377, 403, 452
BstSFI CTRYAG 1 cut(s) 60
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 3 cut(s) 74, 77, 433
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 1 cut(s) 246
BtgI CCRYGG 3 cut(s) 184, 253, 375
Cac8I GCNNGC 1 cut(s) 118
Cfr42I CCGCGG 1 cut(s) 256
CviAII CATG 3 cut(s) 16, 52, 376
CviJI RGCY 9 cut(s) 68, 111, 120, 137, 178, 274, 321, 371, 386
CviKI_1 RGCY 9 cut(s) 68, 111, 120, 137, 178, 274, 321, 371, 386
DdeI CTNAG 2 cut(s) 282, 387
DpnI GATC 2 cut(s) 155, 248
DpnII GATC 2 cut(s) 153, 246
Eco130I CCWWGG 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 3 cut(s) 19, 55, 379
FaiI YATR 8 cut(s) 17, 53, 84, 162, 195, 260, 318, 377
FatI CATG 3 cut(s) 15, 51, 375
Fnu4HI GCNGC 3 cut(s) 63, 66, 447
Fsp4HI GCNGC 3 cut(s) 63, 66, 447
FspBI CTAG 1 cut(s) 117
GluI GCNGC 3 cut(s) 63, 66, 447
GsuI CTGGAG 1 cut(s) 129
Hin1II CATG 3 cut(s) 19, 55, 379
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 2 cut(s) 101, 438
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 290, 329
HpyCH4V TGCA 5 cut(s) 35, 62, 294, 307, 397
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 344, 377, 403, 452
HpyF3I CTNAG 2 cut(s) 282, 387
Hsp92II CATG 3 cut(s) 19, 55, 379
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 2 cut(s) 153, 246
LmnI GCTCC 3 cut(s) 148, 454, 460
LpnPI CCDG 7 cut(s) 90, 126, 159, 170, 186, 417, 456
Lsp1109I GCAGC 3 cut(s) 74, 77, 433
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 148, 323
MalI GATC 2 cut(s) 155, 248
MboI GATC 2 cut(s) 153, 246
MboII GAAGA 2 cut(s) 137, 227
MflI RGATCY 1 cut(s) 246
MluCI AATT 3 cut(s) 199, 232, 341
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 1 cut(s) 285
MseI TTAA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 255
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 117, 344, 377, 403, 452
NcoI CCATGG 1 cut(s) 375
NdeII GATC 2 cut(s) 153, 246
NheI GCTAGC 1 cut(s) 116
NlaIII CATG 3 cut(s) 19, 55, 379
NmuCI GTSAC 1 cut(s) 323
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 3 cut(s) 64, 67, 448
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PstI CTGCAG 1 cut(s) 64
PsuI RGATCY 1 cut(s) 246
SacII CCGCGG 1 cut(s) 256
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 3 cut(s) 63, 66, 447
Sau3AI GATC 2 cut(s) 153, 246
SchI GAGTC 1 cut(s) 110
SetI ASST 6 cut(s) 70, 122, 231, 303, 335, 373
SfcI CTRYAG 1 cut(s) 60
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 3 cut(s) 199, 232, 341
SsiI CCGC 2 cut(s) 253, 255
SspMI CTAG 1 cut(s) 117
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 290, 329
TaqI TCGA 1 cut(s) 436
TasI AATT 3 cut(s) 199, 232, 341
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 3 cut(s) 62, 65, 446
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 173
XapI RAATTY 1 cut(s) 341
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.