pycom16g22710

ECA1 gametogenesis related family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000474
Physical Location & Seq
Reverse (-)
23061477 .. 23062016
540 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g22710.1

Sequence Viewer

Length: 540 bp
ATGGCAAGCTTTGGAAACGCGTTTGTGATGCCGGGGTTGGTGTTAGCAGCAATCATCGTCATGTCATCGGCGAGACTTTCATCTGCATCTGGAGACATTTATGGAAATTATGCTGATCGTGATGAACCATCATCAATAAGCCCTAGCCCTAACCCTAACCCTAGCCCTAGCCCTAGCACCACAGAAGATATCAGCAGCAGCAGCAGCAATTTCCCAGATGTGCTGCCGCTGGAGCCCTCGCAGGGATACTACAGGCATTTGAGGAAATGCGCAGACGAAATAAGCCCTCTGTGCGCTGAACAGATCTTTGAGGGTATGTTCTACAGTAACGAAGAACTGACTGAGGATTGTTGCGCAAACCTTTTGCATGTGGGATACGATTGTCACCGGGGGTTGGTTAAGCTAATCCTCCTAGAACCGACGTTCAAAGGCAAAGCTTCCGAGGCATTGCACAAGAGTTTCCAAATATGGAGCAAGTGTGTCGTAGTAATTCATCAAGGCGACTCACCTTCCCCTGCTGCTGCCGCTGTTTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

180

Amino Acids

19.21

Weight (kDa)

4.86

Isoelectric Point (pI)

68.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 89 - 161 6.9e-13 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000474)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45190 AT1G47450 AT1G47470 AT1G52970 AT2G04031 AT2G04037 AT2G04041 AT2G21655 AT2G21727 AT2G25482 AT3G01185 AT3G11990 AT3G29797 AT3G30247 AT4G13261 AT4G13263 AT5G35405
fragaria_vesca FvH4_3g40551
malus_domestica MD13G1274700.v1.1 MD16G1259400.v1.1
pyrus_communis pycom16g22710
rosa_chinensis RchiOBHm_Chr2g0133141 RchiOBHm_Chr5g0073261 RchiOBHm_Chr5g0073271 RchiOBHm_Chr5g0073281 RchiOBHm_Chr5g0073291 RchiOBHm_Chr5g0073301 RchiOBHm_Chr5g0073311 RchiOBHm_Chr5g0073321 RchiOBHm_Chr5g0073331 RchiOBHm_Chr5g0073341
rosa_laevigata RLG00000002208 RLG00000019311 RLG00000036377 RLG00000036378
rosa_multiflora Rmu_sc0001887.1_g000017 Rmu_sc0001997.1_g000015 Rmu_sc0001997.1_g000017 Rmu_sc0003178.1_g000007 Rmu_sc0005684.1_g000002 Rmu_sc0008365.1_g000001 Rmu_sc0008365.1_g000002 Rmu_sc0017046.1_g000005 Rmu_sc0019942.1_g000001
rosa_roxburghii Rroxscaffold_1G00007900 Rroxscaffold_1G00007930 Rroxscaffold_1G00007950
rosa_rugosa Rorug02G0310300 Rorug05G0422600 Rorug05G0422700 Rorug05G0422800.1 Rorug05G0423600 Rorug05G0423600 Rorug05G0423600 Rorug05G0597300 Rorug05G0597400
rosa_samantha Rh2AG362500 Rh2BG368100 Rh2CG338100 Rh2CG345700 Rh2DG385300 Rh2DG385400 Rh5AG479300 Rh5AG479400 Rh5AG480400 Rh5BG500000 Rh5BG500100 Rh5BG500200 Rh5BG500300 Rh5BG500400 Rh5BG500500 Rh5BG500600 Rh5BG501500 Rh5BG501600 Rh5CG168800 Rh5CG523200 Rh5CG523300 Rh5CG523400 Rh5CG523500 Rh5CG523600 Rh5CG523700 Rh5CG524600 Rh5CG524700 Rh5DG512100 Rh5DG512200 Rh5DG512400 Rh5DG513300 Rh5DG513400 Rh5DG513800 Rh7AG239000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 271, 355
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 227, 525
AfiI CCNNNNNNNGG 2 cut(s) 242, 394
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 1 cut(s) 427
AluBI AGCT 3 cut(s) 9, 403, 437
AluI AGCT 3 cut(s) 9, 403, 437
Alw26I GTCTC 2 cut(s) 67, 87
ApeKI GCWGC 8 cut(s) 47, 195, 198, 201, 204, 223, 518, 521
AspLEI GCGC 3 cut(s) 272, 296, 356
AsuC2I CCSGG 2 cut(s) 33, 389
AsuHPI GGTGA 2 cut(s) 377, 498
BaeI ACNNNNGTAYC 2 cut(s) 367, 400
BanII GRGCYC 1 cut(s) 237
BbvI GCAGC 8 cut(s) 59, 207, 210, 210, 213, 216, 505, 508
BccI CCATC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BciVI GTATCC 2 cut(s) 239, 368
BcnI CCSGG 2 cut(s) 33, 389
BcoDI GTCTC 2 cut(s) 67, 87
BfaI CTAG 5 cut(s) 144, 162, 168, 174, 413
BfmI CTRYAG 2 cut(s) 250, 322
BfuI GTATCC 2 cut(s) 239, 368
BglII AGATCT 1 cut(s) 303
Bme1390I CCNGG 2 cut(s) 33, 389
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 2 cut(s) 33, 389
BmsI GCATC 2 cut(s) 18, 95
BpmI CTGGAG 2 cut(s) 111, 251
BpuMI CCSGG 2 cut(s) 33, 389
BsaJI CCNNGG 3 cut(s) 32, 388, 441
BsaXI ACNNNNNCTCC 2 cut(s) 463, 493
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 394
Bse3DI GCAATG 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 32, 388, 441
BseLI CCNNNNNNNGG 2 cut(s) 242, 394
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 1 cut(s) 333
BseXI GCAGC 8 cut(s) 59, 207, 210, 210, 213, 216, 505, 508
Bsh1236I CGCG 1 cut(s) 20
BsiSI CCGG 2 cut(s) 32, 388
BslI CCNNNNNNNGG 2 cut(s) 242, 394
BsmAI GTCTC 2 cut(s) 67, 87
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 115, 303
BspACI CCGC 2 cut(s) 227, 525
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 446
BssECI CCNNGG 3 cut(s) 32, 388, 441
BssMI GATC 2 cut(s) 115, 303
Bst4CI ACNGT 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 342
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 3 cut(s) 272, 296, 356
BstKTI GATC 2 cut(s) 118, 306
BstMAI GTCTC 2 cut(s) 67, 87
BstMBI GATC 2 cut(s) 115, 303
BstMWI GCNNNNNNNGC 6 cut(s) 201, 204, 232, 291, 443, 524
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 31, 387
BstSFI CTRYAG 2 cut(s) 250, 322
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 8 cut(s) 59, 207, 210, 210, 213, 216, 505, 508
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuI GTATCC 2 cut(s) 239, 368
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 3 cut(s) 272, 296, 356
CviAII CATG 2 cut(s) 61, 368
CviJI RGCY 9 cut(s) 9, 141, 147, 165, 171, 235, 285, 403, 437
CviKI_1 RGCY 9 cut(s) 9, 141, 147, 165, 171, 235, 285, 403, 437
DdeI CTNAG 1 cut(s) 342
DpnI GATC 2 cut(s) 117, 305
DpnII GATC 2 cut(s) 115, 303
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 190
EcoRV GATATC 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 2 cut(s) 64, 371
FaiI YATR 6 cut(s) 62, 102, 111, 317, 369, 469
FatI CATG 2 cut(s) 60, 367
FriOI GRGCYC 1 cut(s) 237
FspBI CTAG 5 cut(s) 144, 162, 168, 174, 413
FspI TGCGCA 2 cut(s) 271, 355
GlaI GCGC 3 cut(s) 271, 295, 355
GsuI CTGGAG 2 cut(s) 111, 251
HapII CCGG 2 cut(s) 32, 388
HhaI GCGC 3 cut(s) 272, 296, 356
Hin1II CATG 2 cut(s) 64, 371
Hin6I GCGC 3 cut(s) 270, 294, 354
HinP1I GCGC 3 cut(s) 270, 294, 354
HindIII AAGCTT 2 cut(s) 7, 435
HinfI GANTC 1 cut(s) 503
HpaII CCGG 2 cut(s) 32, 388
HphI GGTGA 2 cut(s) 377, 498
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 2 cut(s) 90, 119
Hpy99I CGWCG 1 cut(s) 424
HpyAV CCTTC 1 cut(s) 519
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 3 cut(s) 86, 367, 451
HpyF10VI GCNNNNNNNGC 6 cut(s) 201, 204, 232, 291, 443, 524
HpyF3I CTNAG 1 cut(s) 342
HpySE526I ACGT 1 cut(s) 422
Hsp92II CATG 2 cut(s) 64, 371
HspAI GCGC 3 cut(s) 270, 294, 354
Kzo9I GATC 2 cut(s) 115, 303
LmnI GCTCC 2 cut(s) 232, 471
LpnPI CCDG 8 cut(s) 45, 75, 215, 227, 228, 238, 401, 528
Lsp1109I GCAGC 8 cut(s) 59, 207, 210, 210, 213, 216, 505, 508
LweI GCATC 2 cut(s) 18, 95
MaeI CTAG 5 cut(s) 144, 162, 168, 174, 413
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 2 cut(s) 326, 383
MalI GATC 2 cut(s) 117, 305
MboI GATC 2 cut(s) 115, 303
MboII GAAGA 2 cut(s) 197, 344
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 4 cut(s) 106, 208, 489, 535
MluI ACGCGT 1 cut(s) 18
MlyI GAGTC 1 cut(s) 497
MnlI CCTC 7 cut(s) 247, 255, 297, 304, 337, 419, 436
MseI TTAA 2 cut(s) 399, 538
MslI CAYNNNNRTG 1 cut(s) 59
MspA1I CMGCKG 2 cut(s) 229, 527
MspI CCGG 2 cut(s) 32, 388
MspR9I CCNGG 2 cut(s) 33, 389
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 6 cut(s) 201, 204, 232, 291, 443, 524
NciI CCSGG 2 cut(s) 33, 389
NdeII GATC 2 cut(s) 115, 303
NlaIII CATG 2 cut(s) 64, 371
NlaIV GGNNCC 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 383
NsbI TGCGCA 2 cut(s) 271, 355
NspI RCATGY 1 cut(s) 371
PleI GAGTC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 2 cut(s) 399, 538
Sau3AI GATC 2 cut(s) 115, 303
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 2 cut(s) 33, 389
SduI GDGCHC 1 cut(s) 237
SetI ASST 6 cut(s) 11, 363, 405, 425, 439, 511
SfaNI GCATC 2 cut(s) 18, 95
SfcI CTRYAG 2 cut(s) 250, 322
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 4 cut(s) 106, 208, 489, 535
SsiI CCGC 2 cut(s) 227, 525
SspMI CTAG 5 cut(s) 144, 162, 168, 174, 413
StyD4I CCNGG 2 cut(s) 31, 387
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 425
TasI AATT 4 cut(s) 106, 208, 489, 535
TauI GCSGC 2 cut(s) 229, 527
Tru1I TTAA 2 cut(s) 399, 538
Tru9I TTAA 2 cut(s) 399, 538
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 8 cut(s) 47, 195, 198, 201, 204, 223, 518, 521
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 3 cut(s) 69, 138, 482
XceI RCATGY 1 cut(s) 371
XspI CTAG 5 cut(s) 144, 162, 168, 174, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.