AT5G20340

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001086
Physical Location & Seq
Forward (+)
6874787 .. 6875926
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G20340.1

Sequence Viewer

Length: 1065 bp
ATGCTCTATTTGCCTAAGAAACTCTTCTTGTTCTTCTTCTCGTGCATTGTGGTGATTGTCAACTACAACAATAGTGACTTCGTAAACGCAGCAAATAGCATTGGCTTCGTAAACGCAGCAAATAGCATTGGCTTGAACTACGGTCTCCTCGGAGATAACCTCCCATCTCCGTCAAAAGTTATAACCCTTTACAAGTCCATAGACATCACTAAAATCCGAATCTTCGACCCAAACACTGAGGTTCTTAACGCTTTACGTGGCCATCGTGATATTGCGGTCACAGTAGGAGTTAGGGACCAGGACTTGGCTGCTCTTTCAGCTAGCGAAGAAGCTGTTAAGGGCTGGTTTGCGACCAACATCGAGCCTTACTTATCCGACATCAACATCGCGTTCATTACTGTTGGTAACGAAGTCATCCCCGGACCAATCGGTCCTCAAGTGCTTCCAGTCATGCAGTCTCTCACCAACCTCGTCAAGTCAAGGAATCTTCCTATCTCGATAAGCACGGTGGTGGCTATGTGGAACCTCGAGCAATCATACCCACCTTCCGCAGGAATGTTCACGTCTCAAGCGCGTGAACAACTTGTCCCCGTGCTGAAACTATTATCCCAAACAAATTCGCCTATCCTCGTAAAAATCTACCCTTACTTCTCCTATGCGTCCGACCCATCTAGCATCCGTTTGGACTATGCCACCTTCAACACTGAGGCCATCGTGGTACAAGATGGATCACTGGGCTATTCAAACATGTTTGATGCAATCTTTGATGCGTTCGTGTGGGCAATGGAGAAGGAAGGCGTTAAAGATTTACCAATGGTGGTGTCCGAGACCGGATGGCCATCTGCCGGGAATGGAAACATTACCACGCCGGATATCGCGGGTACCTATAACAGAAATTTCGTGAAGCATATAGCAAGCGGAAAAGGTACACCTAAAAGGCCTAACAAAGGCATCGACGGGTTTTTGTTTGCAACTTTCAATGAAAATCAAAAGCCGGTCGGGACTGAACAAAACTTTGGGTTATACAATCCGAATGATATGAAGCCCATCTACAATCTATTCTAA

Protein Analysis

354

Amino Acids

38.88

Weight (kDa)

5.33

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 43 - 353 1.3e-85 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 182
AasI GACNNNNNNGTC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 881
AccB1I GGYRCC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 304
AccII CGCG 3 cut(s) 389, 574, 878
AciI CCGC 4 cut(s) 275, 549, 878, 918
AclWI GGATC 1 cut(s) 736
AcoI YGGCCR 2 cut(s) 259, 836
AcsI RAATTY 2 cut(s) 616, 895
AfaI GTAC 3 cut(s) 720, 883, 928
AfiI CCNNNNNNNGG 4 cut(s) 304, 551, 845, 947
AflIII ACRYGT 1 cut(s) 747
AgsI TTSAA 4 cut(s) 136, 700, 744, 979
AjiI CACGTC 1 cut(s) 564
AjnI CCWGG 1 cut(s) 297
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
AleI CACNNNNGTG 1 cut(s) 509
AloI GAACNNNNNNTCC 2 cut(s) 570, 602
AluBI AGCT 2 cut(s) 320, 332
AluI AGCT 2 cut(s) 320, 332
Alw26I GTCTC 4 cut(s) 149, 462, 570, 821
AlwI GGATC 1 cut(s) 736
Ama87I CYCGRG 1 cut(s) 527
AoxI GGCC 4 cut(s) 259, 708, 836, 938
ApeKI GCWGC 3 cut(s) 89, 116, 308
ApoI RAATTY 2 cut(s) 616, 895
Asp700I GAANNNNTTC 1 cut(s) 23
Asp718I GGTACC 1 cut(s) 881
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 3 cut(s) 295, 422, 431
AsuC2I CCSGG 2 cut(s) 420, 847
AsuHPI GGTGA 2 cut(s) 64, 454
AsuNHI GCTAGC 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 527
AvaII GGWCC 3 cut(s) 295, 422, 431
BalI TGGCCA 2 cut(s) 261, 838
BanI GGYRCC 1 cut(s) 881
BarI GAAGNNNNNNTAC 1 cut(s) 1034
BauI CACGAG 1 cut(s) 40
BbvI GCAGC 3 cut(s) 101, 128, 295
BccI CCATC 8 cut(s) 172, 270, 676, 719, 719, 828, 847, 1055
BcgI CGANNNNNNTGC 2 cut(s) 88, 122
BciT130I CCWGG 1 cut(s) 299
BcnI CCSGG 2 cut(s) 420, 847
BcoDI GTCTC 4 cut(s) 149, 462, 570, 821
BfaI CTAG 2 cut(s) 321, 672
BisI GCNGC 3 cut(s) 90, 117, 309
BlsI GCNGC 3 cut(s) 91, 118, 310
Bme1390I CCNGG 3 cut(s) 299, 420, 847
Bme18I GGWCC 3 cut(s) 295, 422, 431
BmeT110I CYCGRG 1 cut(s) 527
BmgBI CACGTC 1 cut(s) 564
BmgT120I GGNCC 3 cut(s) 295, 422, 431
BmiI GGNNCC 3 cut(s) 296, 524, 883
BmrFI CCNGG 3 cut(s) 299, 420, 847
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 4 cut(s) 684, 745, 757, 960
BmtI GCTAGC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 743
BplI GAGNNNNNCTC 2 cut(s) 144, 176
BpuEI CTTGAG 2 cut(s) 420, 552
BpuMI CCSGG 2 cut(s) 420, 847
BsaAI YACGTR 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 838
BsaI GGTCTC 2 cut(s) 149, 821
BsaJI CCNNGG 2 cut(s) 148, 418
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 551, 845, 947
Bse118I RCCGGY 1 cut(s) 994
Bse1I ACTGG 2 cut(s) 446, 738
Bse3DI GCAATG 1 cut(s) 789
Bse8I GATNNNNATC 1 cut(s) 838
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 148, 418
BseGI GGATG 3 cut(s) 414, 675, 839
BseJI GATNNNNATC 1 cut(s) 838
BseLI CCNNNNNNNGG 4 cut(s) 304, 551, 845, 947
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 2 cut(s) 228, 696
BseNI ACTGG 2 cut(s) 446, 738
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 101, 128, 295
Bsh1236I CGCG 3 cut(s) 389, 574, 878
Bsh1285I CGRYCG 1 cut(s) 999
BshFI GGCC 4 cut(s) 261, 710, 838, 940
BshNI GGYRCC 1 cut(s) 881
BsiEI CGRYCG 1 cut(s) 999
BsiHKCI CYCGRG 1 cut(s) 527
BsiSI CCGG 5 cut(s) 420, 831, 846, 869, 995
BslFI GGGAC 3 cut(s) 308, 572, 1015
BslI CCNNNNNNNGG 4 cut(s) 304, 551, 845, 947
BsmAI GTCTC 4 cut(s) 149, 462, 570, 821
BsmBI CGTCTC 1 cut(s) 570
BsmFI GGGAC 3 cut(s) 308, 572, 1015
BsnI GGCC 4 cut(s) 261, 710, 838, 940
Bso31I GGTCTC 2 cut(s) 149, 821
BsoBI CYCGRG 1 cut(s) 527
Bsp143I GATC 1 cut(s) 728
BspACI CCGC 4 cut(s) 275, 549, 878, 918
BspANI GGCC 4 cut(s) 261, 710, 838, 940
BspCNI CTCAG 2 cut(s) 229, 697
BspFNI CGCG 3 cut(s) 389, 574, 878
BspLI GGNNCC 3 cut(s) 296, 524, 883
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 1 cut(s) 736
BspT107I GGYRCC 1 cut(s) 881
BspTNI GGTCTC 2 cut(s) 149, 821
BsrDI GCAATG 1 cut(s) 789
BsrFI RCCGGY 1 cut(s) 994
BsrI ACTGG 2 cut(s) 446, 738
BssAI RCCGGY 1 cut(s) 994
BssECI CCNNGG 2 cut(s) 148, 418
BssMI GATC 1 cut(s) 728
BssSI CACGAG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 4 cut(s) 143, 283, 400, 508
Bst6I CTCTTC 1 cut(s) 29
BstBAI YACGTR 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 322, 916
BstDEI CTNAG 3 cut(s) 15, 237, 705
BstENI CCTNNNNNAGG 2 cut(s) 549, 945
BstF5I GGATG 3 cut(s) 414, 675, 839
BstFNI CGCG 3 cut(s) 389, 574, 878
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 1 cut(s) 731
BstMAI GTCTC 4 cut(s) 149, 462, 570, 821
BstMBI GATC 1 cut(s) 728
BstMCI CGRYCG 1 cut(s) 999
BstMWI GCNNNNNNNGC 2 cut(s) 10, 317
BstNI CCWGG 1 cut(s) 299
BstNSI RCATGY 1 cut(s) 751
BstSCI CCNGG 3 cut(s) 297, 418, 845
BstUI CGCG 3 cut(s) 389, 574, 878
BstV1I GCAGC 3 cut(s) 101, 128, 295
BsuRI GGCC 4 cut(s) 261, 710, 838, 940
BtgZI GCGATG 1 cut(s) 370
BtrI CACGTC 1 cut(s) 564
BtsCI GGATG 3 cut(s) 414, 675, 839
BtsIMutI CAGTG 3 cut(s) 234, 702, 731
Cac8I GCNNGC 2 cut(s) 322, 916
CfoI GCGC 1 cut(s) 574
Cfr10I RCCGGY 1 cut(s) 994
Cfr13I GGNCC 3 cut(s) 295, 422, 431
CseI GACGC 1 cut(s) 648
Csp6I GTAC 3 cut(s) 719, 882, 927
CviAII CATG 2 cut(s) 451, 748
CviQI GTAC 3 cut(s) 719, 882, 927
DdeI CTNAG 3 cut(s) 15, 237, 705
DpnI GATC 1 cut(s) 730
DpnII GATC 1 cut(s) 728
DrdI GACNNNNNNGTC 1 cut(s) 429
DseDI GACNNNNNNGTC 1 cut(s) 429
EaeI YGGCCR 2 cut(s) 259, 836
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Eco147I AGGCCT 1 cut(s) 940
Eco31I GGTCTC 2 cut(s) 149, 821
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 3 cut(s) 295, 422, 431
Eco88I CYCGRG 1 cut(s) 527
EcoNI CCTNNNNNAGG 2 cut(s) 549, 945
EcoRII CCWGG 1 cut(s) 297
EcoRV GATATC 1 cut(s) 874
Esp3I CGTCTC 1 cut(s) 570
FaeI CATG 2 cut(s) 454, 751
FalI AAGNNNNNCTT 2 cut(s) 8, 40
FaqI GGGAC 3 cut(s) 308, 572, 1015
FatI CATG 2 cut(s) 450, 747
FauI CCCGC 1 cut(s) 871
Fnu4HI GCNGC 3 cut(s) 90, 117, 309
FokI GGATG 3 cut(s) 401, 662, 846
Fsp4HI GCNGC 3 cut(s) 90, 117, 309
FspBI CTAG 2 cut(s) 321, 672
GlaI GCGC 1 cut(s) 573
GluI GCNGC 3 cut(s) 90, 117, 309
HaeIII GGCC 4 cut(s) 261, 710, 838, 940
HapII CCGG 5 cut(s) 420, 831, 846, 869, 995
HgaI GACGC 1 cut(s) 648
HhaI GCGC 1 cut(s) 574
Hin1II CATG 2 cut(s) 454, 751
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 2 cut(s) 219, 484
HpaII CCGG 5 cut(s) 420, 831, 846, 869, 995
HphI GGTGA 2 cut(s) 64, 454
Hpy166II GTNNAC 6 cut(s) 61, 85, 112, 561, 578, 929
Hpy188I TCNGA 6 cut(s) 152, 218, 376, 664, 826, 1032
Hpy188III TCNNGA 4 cut(s) 266, 496, 901, 1000
Hpy8I GTNNAC 6 cut(s) 61, 85, 112, 561, 578, 929
Hpy99I CGWCG 1 cut(s) 959
HpyAV CCTTC 4 cut(s) 555, 706, 784, 788
HpyCH4III ACNGT 4 cut(s) 143, 283, 400, 508
HpyCH4IV ACGT 2 cut(s) 256, 563
HpyCH4V TGCA 4 cut(s) 45, 454, 758, 971
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 317
HpyF3I CTNAG 3 cut(s) 15, 237, 705
HpySE526I ACGT 2 cut(s) 256, 563
Hsp92II CATG 2 cut(s) 454, 751
HspAI GCGC 1 cut(s) 572
KpnI GGTACC 1 cut(s) 885
Kzo9I GATC 1 cut(s) 728
Lsp1109I GCAGC 3 cut(s) 101, 128, 295
LweI GCATC 4 cut(s) 684, 745, 757, 960
MaeI CTAG 2 cut(s) 321, 672
MaeII ACGT 2 cut(s) 256, 563
MaeIII GTNAC 3 cut(s) 74, 277, 404
MalI GATC 1 cut(s) 730
MboI GATC 1 cut(s) 728
MboII GAAGA 6 cut(s) 16, 25, 28, 214, 338, 479
MlsI TGGCCA 2 cut(s) 261, 838
MluCI AATT 2 cut(s) 616, 895
MluNI TGGCCA 2 cut(s) 261, 838
MmeI TCCRAC 2 cut(s) 399, 687
MnlI CCTC 8 cut(s) 158, 170, 232, 444, 479, 536, 638, 700
Mox20I TGGCCA 2 cut(s) 261, 838
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 2 cut(s) 261, 838
MseI TTAA 3 cut(s) 246, 336, 801
MslI CAYNNNNRTG 2 cut(s) 50, 509
Msp20I TGGCCA 2 cut(s) 261, 838
MspI CCGG 5 cut(s) 420, 831, 846, 869, 995
MspR9I CCNGG 3 cut(s) 299, 420, 847
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 3 cut(s) 389, 574, 878
MwoI GCNNNNNNNGC 2 cut(s) 10, 317
NciI CCSGG 2 cut(s) 420, 847
NdeII GATC 1 cut(s) 728
NheI GCTAGC 1 cut(s) 320
NlaIII CATG 2 cut(s) 454, 751
NlaIV GGNNCC 3 cut(s) 296, 524, 883
NmuCI GTSAC 2 cut(s) 74, 277
NspI RCATGY 1 cut(s) 751
OliI CACNNNNGTG 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 527
PceI AGGCCT 1 cut(s) 940
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 219, 484
PflMI CCANNNNNTGG 1 cut(s) 304
PkrI GCNGC 3 cut(s) 91, 118, 310
Ppu21I YACGTR 1 cut(s) 257
PscI ACATGT 1 cut(s) 747
PsiI TTATAA 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 296, 524, 883
PspPI GGNCC 3 cut(s) 295, 422, 431
PspXI VCTCGAGB 1 cut(s) 527
RsaI GTAC 3 cut(s) 720, 883, 928
RsaNI GTAC 3 cut(s) 719, 882, 927
RseI CAYNNNNRTG 2 cut(s) 50, 509
SaqAI TTAA 3 cut(s) 246, 336, 801
SatI GCNGC 3 cut(s) 90, 117, 309
Sau3AI GATC 1 cut(s) 728
Sau96I GGNCC 3 cut(s) 295, 422, 431
ScrFI CCNGG 3 cut(s) 299, 420, 847
SfaNI GCATC 4 cut(s) 684, 745, 757, 960
Sfr274I CTCGAG 1 cut(s) 527
SinI GGWCC 3 cut(s) 295, 422, 431
SlaI CTCGAG 1 cut(s) 527
SmiMI CAYNNNNRTG 2 cut(s) 50, 509
SmlI CTYRAG 3 cut(s) 435, 527, 567
SmoI CTYRAG 3 cut(s) 435, 527, 567
Sse9I AATT 2 cut(s) 616, 895
SseBI AGGCCT 1 cut(s) 940
SsiI CCGC 4 cut(s) 275, 549, 878, 918
SspMI CTAG 2 cut(s) 321, 672
StuI AGGCCT 1 cut(s) 940
StyD4I CCNGG 3 cut(s) 297, 418, 845
TaaI ACNGT 4 cut(s) 143, 283, 400, 508
TaiI ACGT 2 cut(s) 259, 566
TaqI TCGA 5 cut(s) 225, 360, 497, 528, 954
TaqII GACCGA 1 cut(s) 419
TasI AATT 2 cut(s) 616, 895
TfiI GAWTC 2 cut(s) 219, 484
Tru1I TTAA 3 cut(s) 246, 336, 801
Tru9I TTAA 3 cut(s) 246, 336, 801
TscAI CASTG 3 cut(s) 241, 709, 738
TseFI GTSAC 2 cut(s) 74, 277
TseI GCWGC 3 cut(s) 89, 116, 308
Tsp45I GTSAC 2 cut(s) 74, 277
TspDTI ATGAA 3 cut(s) 382, 996, 1055
TspGWI ACGGA 2 cut(s) 159, 668
TspRI CASTG 3 cut(s) 241, 709, 738
Van91I CCANNNNNTGG 1 cut(s) 304
VpaK11BI GGWCC 3 cut(s) 295, 422, 431
XagI CCTNNNNNAGG 2 cut(s) 549, 945
XapI RAATTY 2 cut(s) 616, 895
XceI RCATGY 1 cut(s) 751
XhoI CTCGAG 1 cut(s) 527
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 2 cut(s) 321, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.