FvH4_2g14281

Domain of unknown function (DUF4413)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001193
Physical Location & Seq
Forward (+)
12554926 .. 12557315
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14281.t1

Sequence Viewer

Length: 1641 bp
ATGGCCTCCACACAATCAACAATCACTGATAGTGATCTTACTGAAAATGCCGAGAATCAAGTTTCCGCAGTAACTGTGAATGAGAAAAACTCTACACCAATAGAGAATGAGAGTAACTCAACACCTGATACAATTACTGATGAGGTACGTCGAAGGAGAAAGCTTTCATCTAAAGTTTGGAACGATTTTGATAAGCAAGTAATTGGTGGTAAAACAAAAGCTATATGTAATCATTGCAAGACTAAGTTATCGGCAGAGAAAAATCATGGGACATCACATTTGCATGATCATTTGAAGTCTTGTCTGTATAAACGGCAAAGAAAGGTAGATCAAGCGATGTTGAATCCTACAAGGTCTACTGATGGGTCAACTAAGATTGGCACTTATAGCTTCGATCCAGACAATGCCAGGAGGGAACTTGGTAATATGATTATTCTTCATGAGTATCCTTTGGCTATGGTTGATCATGTTGGATTCAAAAGGTTTATATATGTACCATGTCCGCACACTGCTGATGTACTTGCCGATGCTTTGGTGAATTGCTTATTTGATTGGAATCTTGACCGTAAGTTGTCTACGTTAACTGTAGATAATTGCACTACTAATGATGCCATGCTCGATAAGATTATCGAAAAGATTCATGCTACTTCACTTTTGTTGGATGGAAGTTTATTTCACATGCGTTGTTGCGCCCATATATTGAACCTTATTGTTAGGGATGGCTTGGATGTGATCAAAGAAAGTATTGAAAAAATTCGGTCCAGTATTGCATTTTGGACAGCAACACCAAAGAGAGAGGAGAAGTTTGTAGAGGCAGCTAAGCAGTTGAGAGTTCCAAGTACAAAAATGTTAGAGCTCGATTGTAAAACTAGGTGGAACTCGACATATACCATGCTTAACACTGCTATGATCTATAAGGATGTTTTTCCTCGCTTGAAACATCGAGAGCCCTTGTACAAAGATGTGCCCACTGAAGATGATTGGTCAAAAACAAAAGAACTTGTGGAGAAACTGAAGATGTTTCATGATACGACCGAACTATTTTCAGGCAGTGAAGCAACTGCAACAGCTGAGATTGAGAAGTACAAAAGATTACTTTATGACTTAGTGAAAGAATATCAGTCCAGATCTCCTCAAAGTAATGGAGCATCACAAGCTCCAAATCCTTCTTCATCTAGAGTGTCCGTGCTTAAACTTGATTTGGTGAAACAACTAGATGTGTTTGTTTCTCATTCTGCTGTGAGCACACATGTTAAATCTGAGTTGGATCATTATCTAGAGGAGTCTCTCTTGCCTATAAATGATTATACTAATTTTGATATATTGTGTTGGTGGAACTCGAATGGGATTAAATATCCCACTTTGCAAGAAATTGCAAGAGATATTTTGGCTATTCCTGTATCAACTGTTGCTTCGGAATCTTGCTTTAGTACTAGCGGAAGAGTTATAAGCTCTCATAGGAGCCGACTTCATGCAAAGACAATAGAGGCACTGATGTGTGCTCGAGATTGGTTATGGAGTGAGATAAGAGTTTCAAACCCTGAAGACTCTAAAATAACCACAGTTCAAGATGATGTTGAATGCAATGTTTCGGATGAGGATCCATCCAAGGAAGAAGAGAACGTCACCTTGCTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4413 Dimer_Tnp_hAT zf-BED

Protein Analysis

547

Amino Acids

62.04

Weight (kDa)

5.95

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimer_Tnp_hAT PF05699 421 - 505 9.2e-31 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1448
AccI GTMKAC 2 cut(s) 356, 575
AciI CCGC 3 cut(s) 66, 503, 1437
AclWI GGATC 4 cut(s) 389, 1275, 1595, 1608
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 3 cut(s) 993, 1034, 1563
AfaI GTAC 7 cut(s) 147, 495, 519, 841, 956, 1085, 1432
AflIII ACRYGT 1 cut(s) 1249
AgsI TTSAA 9 cut(s) 295, 343, 478, 703, 749, 937, 1536, 1568, 1580
AjnI CCWGG 1 cut(s) 407
AjuI GAANNNNNNNTTGG 2 cut(s) 1153, 1185
AleI CACNNNNGTG 1 cut(s) 1495
AluBI AGCT 8 cut(s) 163, 221, 390, 818, 856, 1070, 1157, 1452
AluI AGCT 8 cut(s) 163, 221, 390, 818, 856, 1070, 1157, 1452
Alw21I GWGCWC 3 cut(s) 858, 1247, 1504
Alw26I GTCTC 1 cut(s) 1290
AlwI GGATC 4 cut(s) 389, 1275, 1595, 1608
AlwNI CAGNNNCTG 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 1503
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 815
ApoI RAATTY 1 cut(s) 753
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
Asp700I GAANNNNTTC 3 cut(s) 163, 636, 753
AspLEI GCGC 1 cut(s) 692
AspS9I GGNCC 1 cut(s) 759
AsuHPI GGTGA 3 cut(s) 547, 1216, 1618
AvaI CYCGRG 1 cut(s) 1503
AvaII GGWCC 1 cut(s) 759
BaeGI GKGCMC 1 cut(s) 969
BamHI GGATCC 1 cut(s) 1600
BanII GRGCYC 2 cut(s) 858, 951
BbsI GAAGAC 1 cut(s) 1551
Bbv12I GWGCWC 3 cut(s) 858, 1247, 1504
BbvI GCAGC 1 cut(s) 827
BccI CCATC 4 cut(s) 356, 656, 713, 1612
BceAI ACGGC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 409
BciVI GTATCC 1 cut(s) 456
BclI TGATCA 3 cut(s) 286, 463, 732
BcoDI GTCTC 1 cut(s) 1290
BfaI CTAG 5 cut(s) 870, 1176, 1214, 1277, 1434
BfmI CTRYAG 1 cut(s) 585
BfuI GTATCC 1 cut(s) 456
BglII AGATCT 1 cut(s) 1127
BisI GCNGC 1 cut(s) 816
BlpI GCTNAGC 1 cut(s) 819
BlsI GCNGC 1 cut(s) 817
BmcAI AGTACT 1 cut(s) 1432
Bme1390I CCNGG 1 cut(s) 409
Bme18I GGWCC 1 cut(s) 759
BmeT110I CYCGRG 1 cut(s) 1503
BmgT120I GGNCC 1 cut(s) 759
BmiI GGNNCC 2 cut(s) 1463, 1602
BmrFI CCNGG 1 cut(s) 409
BmsI GCATC 3 cut(s) 517, 598, 1157
BpiI GAAGAC 1 cut(s) 1551
BplI GAGNNNNNCTC 4 cut(s) 74, 106, 101, 133
Bpu1102I GCTNAGC 1 cut(s) 819
BsaBI GATNNNNATC 4 cut(s) 33, 555, 1272, 1599
BsaJI CCNNGG 1 cut(s) 1608
Bse1I ACTGG 1 cut(s) 762
Bse3DI GCAATG 2 cut(s) 232, 1591
Bse8I GATNNNNATC 4 cut(s) 33, 555, 1272, 1599
BseBI CCWGG 1 cut(s) 409
BseDI CCNNGG 1 cut(s) 1608
BseGI GGATG 6 cut(s) 667, 724, 733, 925, 1600, 1604
BseJI GATNNNNATC 4 cut(s) 33, 555, 1272, 1599
BseMI GCAATG 2 cut(s) 232, 1591
BseMII CTCAG 2 cut(s) 1062, 1251
BseNI ACTGG 1 cut(s) 762
BseRI GAGGAG 3 cut(s) 812, 1122, 1295
BseSI GKGCMC 1 cut(s) 969
BseXI GCAGC 1 cut(s) 827
Bsh1285I CGRYCG 1 cut(s) 1035
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 1035
BsiHKAI GWGCWC 3 cut(s) 858, 1247, 1504
BsiHKCI CYCGRG 1 cut(s) 1503
BslFI GGGAC 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 1290
BsmFI GGGAC 1 cut(s) 283
BsmI GAATGC 1 cut(s) 1586
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 1503
Bsp1286I GDGCHC 5 cut(s) 858, 951, 969, 1247, 1504
Bsp1407I TGTACA 1 cut(s) 954
Bsp1720I GCTNAGC 1 cut(s) 819
BspACI CCGC 3 cut(s) 66, 503, 1437
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 1063, 1252
BspHI TCATGA 2 cut(s) 439, 1024
BspLI GGNNCC 2 cut(s) 1463, 1602
BspPI GGATC 4 cut(s) 389, 1275, 1595, 1608
BsrDI GCAATG 2 cut(s) 232, 1591
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 1 cut(s) 762
BssECI CCNNGG 1 cut(s) 1608
BssT1I CCWWGG 1 cut(s) 1608
Bst2UI CCWGG 1 cut(s) 409
Bst4CI ACNGT 5 cut(s) 76, 566, 586, 1408, 1564
Bst6I CTCTTC 2 cut(s) 1435, 1611
BstAUI TGTACA 1 cut(s) 954
BstDEI CTNAG 6 cut(s) 243, 372, 819, 1071, 1105, 1260
BstF5I GGATG 6 cut(s) 667, 724, 733, 925, 1600, 1604
BstHHI GCGC 1 cut(s) 692
BstMAI GTCTC 1 cut(s) 1290
BstMCI CGRYCG 1 cut(s) 1035
BstMWI GCNNNNNNNGC 2 cut(s) 387, 1154
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 2 cut(s) 682, 1253
BstSCI CCNGG 1 cut(s) 407
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 969
BstV1I GCAGC 1 cut(s) 827
BstV2I GAAGAC 1 cut(s) 1551
BstX2I RGATCY 2 cut(s) 1127, 1600
BstYI RGATCY 2 cut(s) 1127, 1600
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 6 cut(s) 667, 724, 733, 925, 1600, 1604
BtsI GCAGTG 3 cut(s) 507, 900, 1057
BtsIMutI CAGTG 6 cut(s) 24, 507, 900, 969, 1057, 1490
CaiI CAGNNNCTG 1 cut(s) 74
CciI TCATGA 2 cut(s) 439, 1024
CfoI GCGC 1 cut(s) 692
Cfr13I GGNCC 1 cut(s) 759
Csp6I GTAC 7 cut(s) 146, 494, 518, 840, 955, 1084, 1431
CviQI GTAC 7 cut(s) 146, 494, 518, 840, 955, 1084, 1431
DdeI CTNAG 6 cut(s) 243, 372, 819, 1071, 1105, 1260
Eam1104I CTCTTC 2 cut(s) 1435, 1611
EarI CTCTTC 2 cut(s) 1435, 1611
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 1 cut(s) 1608
Eco24I GRGCYC 2 cut(s) 858, 951
Eco47I GGWCC 1 cut(s) 759
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 3 cut(s) 993, 1034, 1563
Eco88I CYCGRG 1 cut(s) 1503
EcoICRI GAGCTC 1 cut(s) 856
EcoRII CCWGG 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 1608
EcoT38I GRGCYC 2 cut(s) 858, 951
ErhI CCWWGG 1 cut(s) 1608
FaqI GGGAC 1 cut(s) 283
FbaI TGATCA 3 cut(s) 286, 463, 732
FblI GTMKAC 2 cut(s) 356, 575
Fnu4HI GCNGC 1 cut(s) 816
FokI GGATG 6 cut(s) 674, 731, 740, 932, 1591, 1607
FriOI GRGCYC 2 cut(s) 858, 951
Fsp4HI GCNGC 1 cut(s) 816
FspBI CTAG 5 cut(s) 870, 1176, 1214, 1277, 1434
GlaI GCGC 1 cut(s) 691
GluI GCNGC 1 cut(s) 816
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 692
Hin6I GCGC 1 cut(s) 690
HinP1I GCGC 1 cut(s) 690
HincII GTYRAC 2 cut(s) 369, 582
HindII GTYRAC 2 cut(s) 369, 582
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 8 cut(s) 55, 343, 474, 556, 637, 1283, 1418, 1547
HpaI GTTAAC 1 cut(s) 582
HphI GGTGA 3 cut(s) 547, 1216, 1618
Hpy166II GTNNAC 4 cut(s) 357, 369, 576, 582
Hpy188I TCNGA 3 cut(s) 1261, 1417, 1594
Hpy8I GTNNAC 4 cut(s) 357, 369, 576, 582
Hpy99I CGWCG 1 cut(s) 153
HpyAV CCTTC 2 cut(s) 147, 1176
HpyCH4III ACNGT 5 cut(s) 76, 566, 586, 1408, 1564
HpyCH4IV ACGT 3 cut(s) 148, 578, 1623
HpyCH4V TGCA 9 cut(s) 237, 283, 597, 770, 1064, 1366, 1376, 1475, 1584
HpyF10VI GCNNNNNNNGC 2 cut(s) 387, 1154
HpyF3I CTNAG 6 cut(s) 243, 372, 819, 1071, 1105, 1260
HpySE526I ACGT 3 cut(s) 148, 578, 1623
HspAI GCGC 1 cut(s) 690
Ksp22I TGATCA 3 cut(s) 286, 463, 732
KspAI GTTAAC 1 cut(s) 582
LmnI GCTCC 3 cut(s) 1145, 1162, 1461
LpnPI CCDG 9 cut(s) 138, 394, 411, 421, 775, 1032, 1138, 1410, 1554
Lsp1109I GCAGC 1 cut(s) 827
LweI GCATC 3 cut(s) 517, 598, 1157
MaeI CTAG 5 cut(s) 870, 1176, 1214, 1277, 1434
MaeII ACGT 3 cut(s) 148, 578, 1623
MaeIII GTNAC 3 cut(s) 70, 113, 1624
MboII GAAGA 8 cut(s) 428, 986, 1027, 1161, 1452, 1556, 1625, 1628
MflI RGATCY 2 cut(s) 1127, 1600
MhlI GDGCHC 5 cut(s) 858, 951, 969, 1247, 1504
MluCI AATT 7 cut(s) 132, 201, 538, 592, 753, 1312, 1371
MlyI GAGTC 2 cut(s) 1292, 1541
MmeI TCCRAC 3 cut(s) 451, 639, 1245
MroXI GAANNNNTTC 3 cut(s) 163, 636, 753
MseI TTAA 5 cut(s) 581, 897, 1191, 1254, 1350
MslI CAYNNNNRTG 3 cut(s) 282, 905, 1495
MspA1I CMGCKG 1 cut(s) 1070
MspR9I CCNGG 1 cut(s) 409
Mva1269I GAATGC 1 cut(s) 1586
MvaI CCWGG 1 cut(s) 409
MwoI GCNNNNNNNGC 2 cut(s) 387, 1154
NlaIV GGNNCC 2 cut(s) 1463, 1602
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 1624
NspI RCATGY 2 cut(s) 682, 1253
OliI CACNNNNGTG 1 cut(s) 1495
PaeR7I CTCGAG 1 cut(s) 1503
PagI TCATGA 2 cut(s) 439, 1024
PciI ACATGT 1 cut(s) 1249
PctI GAATGC 1 cut(s) 1586
PdmI GAANNNNTTC 3 cut(s) 163, 636, 753
PfeI GAWTC 6 cut(s) 55, 343, 474, 556, 637, 1418
PkrI GCNGC 1 cut(s) 817
PleI GAGTC 2 cut(s) 1291, 1541
PpsI GAGTC 2 cut(s) 1291, 1541
PscI ACATGT 1 cut(s) 1249
PsiI TTATAA 1 cut(s) 1448
Psp124BI GAGCTC 1 cut(s) 858
Psp6I CCWGG 1 cut(s) 407
PspGI CCWGG 1 cut(s) 407
PspN4I GGNNCC 2 cut(s) 1463, 1602
PspPI GGNCC 1 cut(s) 759
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 2 cut(s) 1127, 1600
PvuII CAGCTG 1 cut(s) 1070
RsaI GTAC 7 cut(s) 147, 495, 519, 841, 956, 1085, 1432
RsaNI GTAC 7 cut(s) 146, 494, 518, 840, 955, 1084, 1431
RseI CAYNNNNRTG 3 cut(s) 282, 905, 1495
SacI GAGCTC 1 cut(s) 858
SaqAI TTAA 5 cut(s) 581, 897, 1191, 1254, 1350
SatI GCNGC 1 cut(s) 816
Sau96I GGNCC 1 cut(s) 759
ScaI AGTACT 1 cut(s) 1432
SchI GAGTC 2 cut(s) 1292, 1541
ScrFI CCNGG 1 cut(s) 409
SduI GDGCHC 5 cut(s) 858, 951, 969, 1247, 1504
SfaNI GCATC 3 cut(s) 517, 598, 1157
SfcI CTRYAG 1 cut(s) 585
Sfr274I CTCGAG 1 cut(s) 1503
SinI GGWCC 1 cut(s) 759
SlaI CTCGAG 1 cut(s) 1503
SmiMI CAYNNNNRTG 3 cut(s) 282, 905, 1495
SmlI CTYRAG 2 cut(s) 1503, 1634
SmoI CTYRAG 2 cut(s) 1503, 1634
Sse9I AATT 7 cut(s) 132, 201, 538, 592, 753, 1312, 1371
SsiI CCGC 3 cut(s) 66, 503, 1437
SspMI CTAG 5 cut(s) 870, 1176, 1214, 1277, 1434
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 1 cut(s) 407
StyI CCWWGG 1 cut(s) 1608
TaaI ACNGT 5 cut(s) 76, 566, 586, 1408, 1564
TaiI ACGT 3 cut(s) 151, 581, 1626
TaqI TCGA 9 cut(s) 151, 393, 618, 630, 858, 881, 943, 1340, 1504
TaqII GACCGA 2 cut(s) 747, 1049
TasI AATT 7 cut(s) 132, 201, 538, 592, 753, 1312, 1371
TatI WGTACW 5 cut(s) 517, 839, 954, 1083, 1430
TfiI GAWTC 6 cut(s) 55, 343, 474, 556, 637, 1418
Tru1I TTAA 5 cut(s) 581, 897, 1191, 1254, 1350
Tru9I TTAA 5 cut(s) 581, 897, 1191, 1254, 1350
TscAI CASTG 6 cut(s) 31, 514, 907, 976, 1057, 1497
TseFI GTSAC 1 cut(s) 1624
TseI GCWGC 1 cut(s) 815
Tsp45I GTSAC 1 cut(s) 1624
TspDTI ATGAA 6 cut(s) 156, 428, 629, 1013, 1161, 1460
TspGWI ACGGA 1 cut(s) 1174
TspRI CASTG 6 cut(s) 31, 514, 907, 976, 1057, 1497
VpaK11BI GGWCC 1 cut(s) 759
XapI RAATTY 1 cut(s) 753
XbaI TCTAGA 2 cut(s) 1175, 1276
XceI RCATGY 2 cut(s) 682, 1253
XhoI CTCGAG 1 cut(s) 1503
XmiI GTMKAC 2 cut(s) 356, 575
XmnI GAANNNNTTC 3 cut(s) 163, 636, 753
XspI CTAG 5 cut(s) 870, 1176, 1214, 1277, 1434
ZrmI AGTACT 1 cut(s) 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.