FvH4_3g33583

Domain of unknown function (DUF4413)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001193
Physical Location & Seq
Reverse (-)
29118783 .. 29119784
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33583.t1

Sequence Viewer

Length: 795 bp
ATGTTGCAGACTGCAGTGGAGTATAGGATTGTTTTTCCAGCCTTAAAGACTCGGGGGTATAAGGATGTGCCAAGTGAACAAGACTGGAATTTAGTGGTGGAGATTGCTGAAAAGTTGCAATTATTCTTCAAGGCAACTGAACTGCTGTCTGGCACGAAGTATCCCACAGCCAACTTATTATTTCCGAAGATTTGCCAAATTAAGTTGGCTATATCTAAGTGGCTTCAGTCTGATATTTCTGAAGTGGTAGAAATGGCAAAGAAAATGGAAGAAAAATTTGAGAAGTATTGGGGTGTGATCGGAACAATTGCCGTTGCCACTATTCTAGATCCTCGGTATAAGCTCAAGTTGATTGAGTTTTACTTCAAGCAAGTTTATCCTCGTAGGGTTGCGTTGGAAGTTGACAGAGTCAAGAAGTTTGCATATGACTTGGTCAAGGAGTATAGTTCTTGCGCTATATCTCATGCATCTGATAACTCATCTTTGATTGAAAGTACAAGGCCGAGTGATTTTGATTTAGGGGATGACGACATGGATGCTTATGATGTCTTTGTTTCTGGCTCTAGTAAGGATTCTGACAAGTCTGACTTGGAGAGGTATTTGGATGAGACTGTTTTGCCGAGGGTAAATGATAAGGAGTTTGATGTATTATACTGGTGGAAGACAAAGGATGATATTGATGCTGATGAGGAGTCTTGCCATGATAGTGATGTTGAATTTACTCCACATCAAACGGATGATACTAATACACGTAGAGAGAGAGCAGCTAGAACACGTTCTTCTCCTTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4413 Dimer_Tnp_hAT zf-BED

Protein Analysis

265

Amino Acids

30.61

Weight (kDa)

4.89

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 50 - 148 4.9e-27 hAT-like transposase, RNase-H fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 323
AcsI RAATTY 3 cut(s) 88, 275, 716
AcuI CTGAAG 2 cut(s) 209, 261
AfaI GTAC 1 cut(s) 496
AflIII ACRYGT 2 cut(s) 749, 773
AgsI TTSAA 4 cut(s) 130, 367, 491, 716
AluBI AGCT 2 cut(s) 343, 767
AluI AGCT 2 cut(s) 343, 767
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 51
AoxI GGCC 1 cut(s) 500
ApeKI GCWGC 1 cut(s) 764
ApoI RAATTY 3 cut(s) 88, 275, 716
Asp700I GAANNNNTTC 1 cut(s) 775
AspLEI GCGC 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 668
BbvI GCAGC 1 cut(s) 776
BceAI ACGGC 1 cut(s) 296
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciVI GTATCC 1 cut(s) 171
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 3 cut(s) 326, 564, 768
BfmI CTRYAG 1 cut(s) 12
BfuI GTATCC 1 cut(s) 171
BisI GCNGC 1 cut(s) 765
BlsI GCNGC 1 cut(s) 766
BmeT110I CYCGRG 1 cut(s) 51
BmsI GCATC 3 cut(s) 476, 526, 670
BpiI GAAGAC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 752
BsaJI CCNNGG 2 cut(s) 332, 620
Bse1I ACTGG 2 cut(s) 89, 659
BseDI CCNNGG 2 cut(s) 332, 620
BseGI GGATG 6 cut(s) 70, 529, 541, 610, 676, 742
BseNI ACTGG 2 cut(s) 89, 659
BseRI GAGGAG 1 cut(s) 704
BseXI GCAGC 1 cut(s) 776
BshFI GGCC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 51
BsmAI GTCTC 1 cut(s) 602
BsnI GGCC 1 cut(s) 502
BsoBI CYCGRG 1 cut(s) 51
Bsp143I GATC 2 cut(s) 297, 328
BspANI GGCC 1 cut(s) 502
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 1 cut(s) 323
BsrI ACTGG 2 cut(s) 89, 659
BssECI CCNNGG 2 cut(s) 332, 620
BssMI GATC 2 cut(s) 297, 328
Bst4CI ACNGT 1 cut(s) 613
BstBAI YACGTR 1 cut(s) 752
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 6 cut(s) 70, 529, 541, 610, 676, 742
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 2 cut(s) 300, 331
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 2 cut(s) 297, 328
BstSFI CTRYAG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 776
BstV2I GAAGAC 1 cut(s) 668
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuI GTATCC 1 cut(s) 171
BsuRI GGCC 1 cut(s) 502
BtsCI GGATG 6 cut(s) 70, 529, 541, 610, 676, 742
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
CfoI GCGC 1 cut(s) 455
Csp6I GTAC 1 cut(s) 495
CviAII CATG 3 cut(s) 464, 532, 701
CviJI RGCY 8 cut(s) 41, 170, 209, 223, 343, 502, 561, 767
CviKI_1 RGCY 8 cut(s) 41, 170, 209, 223, 343, 502, 561, 767
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 1 cut(s) 216
DpnI GATC 2 cut(s) 299, 330
DpnII GATC 2 cut(s) 297, 328
Eco57I CTGAAG 2 cut(s) 209, 261
Eco88I CYCGRG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 469
FaeI CATG 3 cut(s) 467, 535, 704
FalI AAGNNNNNCTT 2 cut(s) 572, 604
FatI CATG 3 cut(s) 463, 531, 700
FauNDI CATATG 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 765
FokI GGATG 6 cut(s) 77, 536, 548, 617, 683, 749
Fsp4HI GCNGC 1 cut(s) 765
FspBI CTAG 3 cut(s) 326, 564, 768
GlaI GCGC 1 cut(s) 454
GluI GCNGC 1 cut(s) 765
HaeIII GGCC 1 cut(s) 502
HhaI GCGC 1 cut(s) 455
Hin1II CATG 3 cut(s) 467, 535, 704
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 4 cut(s) 49, 408, 572, 692
Hpy166II GTNNAC 2 cut(s) 77, 403
Hpy188I TCNGA 7 cut(s) 186, 232, 241, 302, 472, 577, 586
Hpy188III TCNNGA 2 cut(s) 326, 412
Hpy8I GTNNAC 2 cut(s) 77, 403
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 2 cut(s) 751, 775
HpyCH4V TGCA 5 cut(s) 7, 14, 118, 422, 467
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 2 cut(s) 751, 775
Hsp92II CATG 3 cut(s) 467, 535, 704
HspAI GCGC 1 cut(s) 453
Kzo9I GATC 2 cut(s) 297, 328
LpnPI CCDG 5 cut(s) 51, 70, 135, 543, 640
Lsp1109I GCAGC 1 cut(s) 776
LweI GCATC 3 cut(s) 476, 526, 670
MaeI CTAG 3 cut(s) 326, 564, 768
MaeII ACGT 2 cut(s) 751, 775
MalI GATC 2 cut(s) 299, 330
MboI GATC 2 cut(s) 297, 328
MboII GAAGA 5 cut(s) 118, 199, 281, 673, 771
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 1 cut(s) 328
MluCI AATT 6 cut(s) 88, 119, 198, 275, 306, 716
MlyI GAGTC 3 cut(s) 43, 417, 701
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 5 cut(s) 342, 390, 588, 615, 682
Mph1103I ATGCAT 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 775
MseI TTAA 2 cut(s) 44, 201
MslI CAYNNNNRTG 1 cut(s) 705
MunI CAATTG 1 cut(s) 306
NdeI CATATG 1 cut(s) 424
NdeII GATC 2 cut(s) 297, 328
NlaIII CATG 3 cut(s) 467, 535, 704
NmeAIII GCCGAG 2 cut(s) 528, 645
NsiI ATGCAT 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 775
PfeI GAWTC 1 cut(s) 572
PflFI GACNNNGTC 2 cut(s) 407, 431
PkrI GCNGC 1 cut(s) 766
PleI GAGTC 3 cut(s) 43, 416, 700
PpsI GAGTC 3 cut(s) 43, 416, 700
Ppu21I YACGTR 1 cut(s) 752
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 328
PsyI GACNNNGTC 2 cut(s) 407, 431
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
RseI CAYNNNNRTG 1 cut(s) 705
SaqAI TTAA 2 cut(s) 44, 201
SatI GCNGC 1 cut(s) 765
Sau3AI GATC 2 cut(s) 297, 328
SchI GAGTC 3 cut(s) 43, 417, 701
SetI ASST 5 cut(s) 345, 599, 754, 769, 778
SfaNI GCATC 3 cut(s) 476, 526, 670
SfcI CTRYAG 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 6 cut(s) 88, 119, 198, 275, 306, 716
SspMI CTAG 3 cut(s) 326, 564, 768
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 2 cut(s) 754, 778
TasI AATT 6 cut(s) 88, 119, 198, 275, 306, 716
TatI WGTACW 1 cut(s) 494
TfiI GAWTC 1 cut(s) 572
Tru1I TTAA 2 cut(s) 44, 201
Tru9I TTAA 2 cut(s) 44, 201
TscAI CASTG 1 cut(s) 21
TseI GCWGC 1 cut(s) 764
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 1 cut(s) 21
Tth111I GACNNNGTC 2 cut(s) 407, 431
XapI RAATTY 3 cut(s) 88, 275, 716
XbaI TCTAGA 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 775
XspI CTAG 3 cut(s) 326, 564, 768
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.