Rroxscaffold_1G00017280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001193
Physical Location & Seq
Forward (+)
21428226 .. 21436030
7805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00017280.1

Sequence Viewer

Length: 516 bp
ATGGTGATGAGATTTGATGGATACAAAGACGCCTATATTGTCATTAGAGAATTGAATGGCGGTGTGGGCGACGAGCTTGATACCGCGATCCTTGGGGCCTTTGGTGGTGCAAACCTCGTCGTGGTCGTCCTTCGGCCGGTGGCCCTTAGAATGTGGCCTCGAGCCTCCACGATTTCGCTAGTGGTGGAGGAAGAAGCGCCTTTATCCCCAAACCCAATCGAGAAGCAGCGGAGGATGACGTTGTTGTGCGGTGGTGGAGGTTGTTGTGGTCAAATCATTGGCGACGGCGGCGGGGATGAACTCGGAGTCCCACAACCGTCGATTACAACCCAATCCAACGGTCACTCCTCTCCCCAGCTCTTCTATCCCCTCAACAAATACAAGAATCAGCCCAATATAAAAACCCAACCCCTCCCACGAAAATTCTCAAATCCACTGTCCGAAAATCTCCCAATCCTCTCTCTCCAATTTGTGAATTCTCTCTCAAAAGGGTATCAGATTCAGCGCTCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.56

Weight (kDa)

7.69

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 86
AciI CCGC 6 cut(s) 60, 84, 229, 249, 288, 291
AclWI GGATC 1 cut(s) 82
AcoI YGGCCR 1 cut(s) 134
AcsI RAATTY 2 cut(s) 422, 475
AcyI GRCGYC 1 cut(s) 30
AfeI AGCGCT 1 cut(s) 506
AfiI CCNNNNNNNGG 2 cut(s) 121, 136
AgsI TTSAA 1 cut(s) 55
AloI GAACNNNNNNTCC 2 cut(s) 291, 323
AluBI AGCT 2 cut(s) 76, 358
AluI AGCT 2 cut(s) 76, 358
AlwI GGATC 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 159
Aor51HI AGCGCT 1 cut(s) 506
AoxI GGCC 4 cut(s) 96, 134, 141, 155
ApeKI GCWGC 1 cut(s) 226
ApoI RAATTY 2 cut(s) 422, 475
ArsI GACNNNNNNTTYG 2 cut(s) 422, 454
AspLEI GCGC 2 cut(s) 199, 507
AspS9I GGNCC 2 cut(s) 96, 142
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 238
BccI CCATC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 301
BciVI GTATCC 1 cut(s) 14
BfaI CTAG 1 cut(s) 179
BfoI RGCGCY 2 cut(s) 200, 508
BfuI GTATCC 1 cut(s) 14
BisI GCNGC 2 cut(s) 227, 289
BlsI GCNGC 2 cut(s) 228, 290
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 96, 142
BmiI GGNNCC 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 136
Bse118I RCCGGY 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 2 cut(s) 240, 301
BseLI CCNNNNNNNGG 2 cut(s) 121, 136
BseRI GAGGAG 1 cut(s) 337
BseX3I CGGCCG 1 cut(s) 134
BseXI GCAGC 1 cut(s) 238
BseYI CCCAGC 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 86
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 4 cut(s) 98, 136, 143, 157
BsiEI CGRYCG 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 121, 136
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 4 cut(s) 98, 136, 143, 157
BsoBI CYCGRG 1 cut(s) 159
Bsp143I GATC 1 cut(s) 87
BspACI CCGC 6 cut(s) 60, 84, 229, 249, 288, 291
BspANI GGCC 4 cut(s) 98, 136, 143, 157
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 82
BspQI GCTCTTC 1 cut(s) 365
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 1 cut(s) 87
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 91
Bst4CI ACNGT 3 cut(s) 318, 341, 438
Bst6I CTCTTC 1 cut(s) 365
BstACI GRCGYC 1 cut(s) 30
BstDEI CTNAG 2 cut(s) 146, 508
BstF5I GGATG 2 cut(s) 240, 301
BstFNI CGCG 1 cut(s) 86
BstH2I RGCGCY 2 cut(s) 200, 508
BstHHI GCGC 2 cut(s) 199, 507
BstKTI GATC 1 cut(s) 90
BstMBI GATC 1 cut(s) 87
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 2 cut(s) 66, 288
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 238
BstZI CGGCCG 1 cut(s) 134
BsuI GTATCC 1 cut(s) 14
BsuRI GGCC 4 cut(s) 98, 136, 143, 157
BtsCI GGATG 2 cut(s) 240, 301
BtsIMutI CAGTG 1 cut(s) 434
CfoI GCGC 2 cut(s) 199, 507
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 96, 142
CseI GACGC 1 cut(s) 38
CviJI RGCY 8 cut(s) 76, 98, 136, 143, 157, 164, 358, 391
CviKI_1 RGCY 8 cut(s) 76, 98, 136, 143, 157, 164, 358, 391
DdeI CTNAG 2 cut(s) 146, 508
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 134
EagI CGGCCG 1 cut(s) 134
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
EclXI CGGCCG 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 506
Eco52I CGGCCG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 96
EcoRI GAATTC 1 cut(s) 475
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaiI YATR 2 cut(s) 36, 398
FaqI GGGAC 1 cut(s) 293
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 2 cut(s) 227, 289
FokI GGATG 2 cut(s) 247, 308
Fsp4HI GCNGC 2 cut(s) 227, 289
FspBI CTAG 1 cut(s) 179
GlaI GCGC 2 cut(s) 198, 506
GluI GCNGC 2 cut(s) 227, 289
GsaI CCCAGC 1 cut(s) 358
HaeII RGCGCY 2 cut(s) 200, 508
HaeIII GGCC 4 cut(s) 98, 136, 143, 157
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 38
HhaI GCGC 2 cut(s) 199, 507
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 2 cut(s) 197, 505
HinP1I GCGC 2 cut(s) 197, 505
HinfI GANTC 3 cut(s) 306, 385, 499
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 4 cut(s) 305, 442, 498, 511
Hpy188III TCNNGA 1 cut(s) 220
Hpy99I CGWCG 4 cut(s) 74, 122, 287, 322
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 3 cut(s) 318, 341, 438
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 1 cut(s) 110
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 288
HpyF3I CTNAG 2 cut(s) 146, 508
HpySE526I ACGT 1 cut(s) 239
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 2 cut(s) 197, 505
Kzo9I GATC 1 cut(s) 87
LguI GCTCTTC 1 cut(s) 365
LpnPI CCDG 2 cut(s) 150, 368
Lsp1109I GCAGC 1 cut(s) 238
MaeI CTAG 1 cut(s) 179
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 341
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 2 cut(s) 203, 352
MluCI AATT 4 cut(s) 50, 422, 467, 475
MlyI GAGTC 1 cut(s) 315
MmeI TCCRAC 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 229
MspI CCGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 86
MwoI GCNNNNNNNGC 2 cut(s) 66, 288
NdeII GATC 1 cut(s) 87
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 341
PaeR7I CTCGAG 1 cut(s) 159
PciSI GCTCTTC 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 2 cut(s) 385, 499
PkrI GCNGC 2 cut(s) 228, 290
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 96, 142
PspXI VCTCGAGB 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 365
SatI GCNGC 2 cut(s) 227, 289
Sau3AI GATC 1 cut(s) 87
Sau96I GGNCC 2 cut(s) 96, 142
SchI GAGTC 1 cut(s) 315
SetI ASST 5 cut(s) 78, 117, 242, 262, 360
Sfr274I CTCGAG 1 cut(s) 159
SlaI CTCGAG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 159
SmoI CTYRAG 1 cut(s) 159
Sse9I AATT 4 cut(s) 50, 422, 467, 475
SsiI CCGC 6 cut(s) 60, 84, 229, 249, 288, 291
SspMI CTAG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 318, 341, 438
TaiI ACGT 1 cut(s) 242
TaqI TCGA 3 cut(s) 160, 219, 320
TasI AATT 4 cut(s) 50, 422, 467, 475
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 2 cut(s) 385, 499
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 1 cut(s) 441
XapI RAATTY 2 cut(s) 422, 475
XhoI CTCGAG 1 cut(s) 159
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.