Rorug05G0035700

BED zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001193
Physical Location & Seq
Forward (+)
2494241 .. 2498191
3951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0035700.1

Sequence Viewer

Length: 1884 bp
ATGTCGGCGTACCGATGGAAGTCCTTTCACGAGAACGAAGACCGGCCGGAGAAGCCTCGCCGGTACGGCGTCACAGAAATGAGAGGCCCCAATCACACTCTCTTGACCCAAAACGTCCTTCAGGATATTTTCGAGTCGATGGGGGACATTGTGGATGGGCTCAAGTTTGCTGGAGGTTCGCATAGCTTGATGCCGAAGTCGTTTGTTAAGGAGGTGATTGATGTGGCTCACCAACATAACGTGTATGTAAGCACTGGTGATTGGGCTGAGAATTTACTTCGAAAAGGCCCTTCGGCTTTTAAAGAGTATGTGGAGGAGTGTAAGAGCTTGGGATTTGACACAATTGAGCTGAATGTGGGATCTCTCGGAATGCCTGAGGAAACGCTTCTGAGACTTGTGCGCTTGATTAAGAGCGGTGGCTTGAAGGTGAAGCCTCTGTTTGAAGTCAAGGTTAACGAGTCTGACATTCCTATTGGTGATAGAGCGTTCGGAGCCTATGTTGTCCCGAGACCAAGATCATCTGAATTTGTTGAAGATGTTGATCTGTTGATTAGAAGGGCTGAGAGATGCTTAGAAGCTGGGGCTGACATGATAATGATTGAGGCTGATGATGTCTGTAAACAGGCTGATTCAATGCGGCCAGACATAATTGCAAAGGTCATTGGGCGCCTTGGTGTCGAGAAGACCATGTTTGAAGCATCAAATCCAAGAACCTCGGAGTGGTTCATCCAGCATTATGGTCCAAGGGTCAATCTGTTTGTGGACCACTCTCATGTGATGGACCTGGAGTGCCTCCGGGGACGCAACTTAGTAAAATTTGCTCTGTCACAGTACGATTCAACCTTCGAATTTGAATACTATTTGAATACTATCTATGGAGCTCAAACCTCCACCTTCATATATTTTTCCATTCTACTGAGAACACCCGGCCATGGTTTACCAAAGGTTGTGAACTGCGATAGGTCAGATAGTAGCTGCAGAAACACTCTTTGGATAAGGATAGTACCTTCCATGTTGTGGTATTCCGGAAGCAAGGTAATTGAGGAAGAACACGATTCTGAGACAGCTACTTTAGCGTGTACCATTGATGTCTATGAATTATCAGGACTACATTCTCCTCTATATCCATCTCAAAACTGGAAAGTTTTCACAATCGTGCTAGAAGTGCGAGATCTAGAAGATCGTTGCCGTCAACATGTCTCCAGGTGTGCCGGTCTCACTGAACTATATGGAGATGAAAAGAAAGCAGTAGAACTTGATTGCCAGCAAGGGTTGAAGAAGGAAGGCGCGTTGAAGATCAAGAGGGTGTTTGAAGTTAATAATATACCGGAGACCTTTTCTGAGTTCGAAGAATATAGACAAGCGGTGATGACAAGAGCTAGGAATCCACCATCTTCCCAGGCTAATGAGGTGAACTTTCCCGGGTTTGAAGAGGAAAAAGGACAAGGGGTGGGAAGGCTGAATGAACCAGCATATTGCCGGGCTAATGGGAATGAGCTTCAAAGGTACTATGGCACAACTGTCTCATGCTCACTTAGCAGCAGCTCGAGCAGTAGTTCTACTTCTACCATAGATTTATGCGATTCGTTTCAATGTCGTTTATGTTTGGTTCTCAAAGAGGGGTTGGGACAGGCAGTGACCACCTCCACCGCTGTAACGGCCATGCTTAGAAACAAGCATGTTACGTTAAGCCGGGGGCAAAAGAAGGCAGTACTAATGTGCAGAGTGATTGCCGGGAAGGTAAAGCCTAGGTTGTGCTCTCAGCGAAAGATTGCCCCTACTTTGGATCAGTTTGATGCAGTGGCTCTTAATGACCACGATCTATACGTACAAGATTCCAGAGCTATTCTACCTTGTTTTGTGGTGATTTGCGAAGGCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

627

Amino Acids

70.6

Weight (kDa)

5.96

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ComA PF02679 14 - 268 1.2e-73 (2R)-phospho-3-sulfolactate synthase (ComA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 666
AccB7I CCANNNNNTGG 1 cut(s) 1017
AccBSI CCGCTC 1 cut(s) 414
AccII CGCG 1 cut(s) 1289
AccIII TCCGGA 1 cut(s) 1025
AciI CCGC 4 cut(s) 414, 637, 1364, 1650
AclWI GGATC 2 cut(s) 367, 1794
AcoI YGGCCR 4 cut(s) 44, 638, 928, 1659
AcsI RAATTY 4 cut(s) 271, 524, 815, 848
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 69, 667
AfaI GTAC 8 cut(s) 11, 65, 833, 1005, 1081, 1508, 1713, 1830
AfiI CCNNNNNNNGG 4 cut(s) 720, 932, 1017, 1783
AflIII ACRYGT 2 cut(s) 240, 1195
AjnI CCWGG 3 cut(s) 783, 1202, 1398
AjuI GAANNNNNNNTTGG 4 cut(s) 102, 134, 973, 1005
AleI CACNNNNGTG 1 cut(s) 1154
Alw21I GWGCWC 2 cut(s) 883, 1760
Alw26I GTCTC 7 cut(s) 385, 502, 1055, 1204, 1220, 1325, 1528
AlwI GGATC 2 cut(s) 367, 1794
Ama87I CYCGRG 3 cut(s) 505, 1421, 1546
Aor13HI TCCGGA 1 cut(s) 1025
AoxI GGCC 6 cut(s) 44, 85, 286, 638, 928, 1659
ApeKI GCWGC 3 cut(s) 975, 1539, 1542
ApoI RAATTY 4 cut(s) 271, 524, 815, 848
Asp700I GAANNNNTTC 2 cut(s) 384, 1145
AspA2I CCTAGG 1 cut(s) 1748
AspLEI GCGC 3 cut(s) 402, 669, 1289
AspS9I GGNCC 5 cut(s) 86, 287, 740, 763, 781
AsuC2I CCSGG 7 cut(s) 797, 927, 1422, 1423, 1481, 1694, 1735
AsuHPI GGTGA 8 cut(s) 221, 226, 269, 439, 488, 1378, 1423, 1876
AsuII TTCGAA 3 cut(s) 280, 846, 1347
AvaI CYCGRG 3 cut(s) 505, 1421, 1546
AvaII GGWCC 3 cut(s) 740, 763, 781
AvrII CCTAGG 1 cut(s) 1748
AxyI CCTNAGG 1 cut(s) 375
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 2 cut(s) 162, 883
BarI GAAGNNNNNNTAC 2 cut(s) 1546, 1578
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 2 cut(s) 45, 689
Bbv12I GWGCWC 2 cut(s) 883, 1760
BbvI GCAGC 3 cut(s) 962, 1551, 1554
BccI CCATC 6 cut(s) 9, 133, 149, 772, 1135, 1399
BceAI ACGGC 3 cut(s) 82, 1173, 1674
BciT130I CCWGG 3 cut(s) 785, 1204, 1400
BcnI CCSGG 7 cut(s) 797, 927, 1422, 1423, 1481, 1694, 1735
BcoDI GTCTC 7 cut(s) 385, 502, 1055, 1204, 1220, 1325, 1528
BfaI CTAG 4 cut(s) 1160, 1175, 1380, 1749
BfmI CTRYAG 1 cut(s) 976
BfoI RGCGCY 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 66
BglII AGATCT 1 cut(s) 1171
BisI GCNGC 4 cut(s) 638, 976, 1540, 1543
BlnI CCTAGG 1 cut(s) 1748
BlsI GCNGC 4 cut(s) 639, 977, 1541, 1544
BmcAI AGTACT 1 cut(s) 1713
Bme18I GGWCC 3 cut(s) 740, 763, 781
BmeT110I CYCGRG 3 cut(s) 505, 1421, 1546
BmgT120I GGNCC 5 cut(s) 86, 287, 740, 763, 781
BmiI GGNNCC 3 cut(s) 88, 493, 668
BmsI GCATC 4 cut(s) 180, 557, 707, 1786
BpiI GAAGAC 2 cut(s) 45, 689
BpmI CTGGAG 3 cut(s) 192, 806, 1186
Bpu14I TTCGAA 3 cut(s) 280, 846, 1347
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 7 cut(s) 797, 927, 1422, 1423, 1481, 1694, 1735
BsaAI YACGTR 1 cut(s) 1828
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 2 cut(s) 69, 667
BsaI GGTCTC 3 cut(s) 502, 1220, 1325
BsaJI CCNNGG 9 cut(s) 670, 714, 743, 796, 931, 1398, 1421, 1693, 1748
BsaWI WCCGGW 2 cut(s) 1025, 1327
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 4 cut(s) 720, 932, 1017, 1783
Bse118I RCCGGY 3 cut(s) 42, 60, 1211
Bse1I ACTGG 2 cut(s) 259, 1142
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 1025
BseBI CCWGG 3 cut(s) 785, 1204, 1400
BseDI CCNNGG 9 cut(s) 670, 714, 743, 796, 931, 1398, 1421, 1693, 1748
BseGI GGATG 2 cut(s) 160, 726
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 4 cut(s) 720, 932, 1017, 1783
BseMII CTCAG 8 cut(s) 258, 366, 380, 552, 908, 1050, 1332, 1775
BseNI ACTGG 2 cut(s) 259, 1142
BseRI GAGGAG 2 cut(s) 329, 1107
BseX3I CGGCCG 1 cut(s) 44
BseXI GCAGC 3 cut(s) 962, 1551, 1554
BseYI CCCAGC 1 cut(s) 578
BsgI GTGCAG 1 cut(s) 1741
Bsh1236I CGCG 1 cut(s) 1289
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 6 cut(s) 46, 87, 288, 640, 930, 1661
BshNI GGYRCC 1 cut(s) 666
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 2 cut(s) 883, 1760
BsiHKCI CYCGRG 3 cut(s) 505, 1421, 1546
BslFI GGGAC 4 cut(s) 158, 488, 813, 1641
BslI CCNNNNNNNGG 4 cut(s) 720, 932, 1017, 1783
BsmAI GTCTC 7 cut(s) 385, 502, 1055, 1204, 1220, 1325, 1528
BsmFI GGGAC 4 cut(s) 158, 488, 813, 1641
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 6 cut(s) 46, 87, 288, 640, 930, 1661
Bso31I GGTCTC 3 cut(s) 502, 1220, 1325
BsoBI CYCGRG 3 cut(s) 505, 1421, 1546
Bsp119I TTCGAA 3 cut(s) 280, 846, 1347
Bsp1286I GDGCHC 3 cut(s) 162, 883, 1760
Bsp13I TCCGGA 1 cut(s) 1025
Bsp143I GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
Bsp19I CCATGG 1 cut(s) 931
BspACI CCGC 4 cut(s) 414, 637, 1364, 1650
BspANI GGCC 6 cut(s) 46, 87, 288, 640, 930, 1661
BspCNI CTCAG 8 cut(s) 259, 367, 381, 553, 909, 1051, 1333, 1774
BspEI TCCGGA 1 cut(s) 1025
BspFNI CGCG 1 cut(s) 1289
BspLI GGNNCC 3 cut(s) 88, 493, 668
BspMAI CTGCAG 1 cut(s) 980
BspPI GGATC 2 cut(s) 367, 1794
BspT104I TTCGAA 3 cut(s) 280, 846, 1347
BspT107I GGYRCC 1 cut(s) 666
BspTNI GGTCTC 3 cut(s) 502, 1220, 1325
BsrBI CCGCTC 1 cut(s) 414
BsrFI RCCGGY 3 cut(s) 42, 60, 1211
BsrI ACTGG 2 cut(s) 259, 1142
BssAI RCCGGY 3 cut(s) 42, 60, 1211
BssECI CCNNGG 9 cut(s) 670, 714, 743, 796, 931, 1398, 1421, 1693, 1748
BssMI GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
BssNI GRCGYC 2 cut(s) 69, 667
BssSI CACGAG 1 cut(s) 29
BssT1I CCWWGG 4 cut(s) 670, 743, 931, 1748
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 3 cut(s) 785, 1204, 1400
Bst4CI ACNGT 2 cut(s) 831, 1522
Bst6I CTCTTC 1 cut(s) 1425
BstACI GRCGYC 2 cut(s) 69, 667
BstBAI YACGTR 1 cut(s) 1828
BstBI TTCGAA 3 cut(s) 280, 846, 1347
BstC8I GCNNGC 1 cut(s) 1265
BstDSI CCRYGG 1 cut(s) 931
BstF5I GGATG 2 cut(s) 160, 726
BstFNI CGCG 1 cut(s) 1289
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 3 cut(s) 402, 669, 1289
BstKTI GATC 8 cut(s) 362, 518, 544, 1174, 1183, 1299, 1789, 1822
BstMAI GTCTC 7 cut(s) 385, 502, 1055, 1204, 1220, 1325, 1528
BstMBI GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 8 cut(s) 52, 66, 491, 1073, 1165, 1536, 1548, 1658
BstNI CCWGG 3 cut(s) 785, 1204, 1400
BstNSI RCATGY 2 cut(s) 1199, 1682
BstSFI CTRYAG 1 cut(s) 976
BstSNI TACGTA 1 cut(s) 1828
BstUI CGCG 1 cut(s) 1289
BstV1I GCAGC 3 cut(s) 962, 1551, 1554
BstV2I GAAGAC 2 cut(s) 45, 689
BstX2I RGATCY 2 cut(s) 359, 1171
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 2 cut(s) 359, 1171
BstZI CGGCCG 1 cut(s) 44
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 6 cut(s) 46, 87, 288, 640, 930, 1661
BtgI CCRYGG 1 cut(s) 931
BtsCI GGATG 2 cut(s) 160, 726
BtsI GCAGTG 2 cut(s) 1641, 1806
BtsIMutI CAGTG 4 cut(s) 252, 1218, 1641, 1806
Cac8I GCNNGC 1 cut(s) 1265
CfoI GCGC 3 cut(s) 402, 669, 1289
Cfr10I RCCGGY 3 cut(s) 42, 60, 1211
Cfr13I GGNCC 5 cut(s) 86, 287, 740, 763, 781
Cfr9I CCCGGG 1 cut(s) 1421
CseI GACGC 2 cut(s) 58, 810
Csp6I GTAC 8 cut(s) 10, 64, 832, 1004, 1080, 1507, 1712, 1829
CviAII CATG 9 cut(s) 589, 688, 773, 932, 1012, 1196, 1527, 1663, 1679
CviQI GTAC 8 cut(s) 10, 64, 832, 1004, 1080, 1507, 1712, 1829
DinI GGCGCC 1 cut(s) 668
DpnI GATC 8 cut(s) 361, 517, 543, 1173, 1182, 1298, 1788, 1821
DpnII GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
DraI TTTAAA 1 cut(s) 301
EaeI YGGCCR 4 cut(s) 44, 638, 928, 1659
EagI CGGCCG 1 cut(s) 44
Eam1104I CTCTTC 1 cut(s) 1425
EarI CTCTTC 1 cut(s) 1425
Ecl136II GAGCTC 1 cut(s) 881
EclXI CGGCCG 1 cut(s) 44
Eco105I TACGTA 1 cut(s) 1828
Eco130I CCWWGG 4 cut(s) 670, 743, 931, 1748
Eco24I GRGCYC 2 cut(s) 162, 883
Eco31I GGTCTC 3 cut(s) 502, 1220, 1325
Eco47I GGWCC 3 cut(s) 740, 763, 781
Eco52I CGGCCG 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 881
Eco57I CTGAAG 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 3 cut(s) 505, 1421, 1546
EcoICRI GAGCTC 1 cut(s) 881
EcoO109I RGGNCCY 2 cut(s) 86, 287
EcoRII CCWGG 3 cut(s) 783, 1202, 1398
EcoT14I CCWWGG 4 cut(s) 670, 743, 931, 1748
EcoT38I GRGCYC 2 cut(s) 162, 883
EgeI GGCGCC 1 cut(s) 668
EheI GGCGCC 1 cut(s) 668
ErhI CCWWGG 4 cut(s) 670, 743, 931, 1748
FaeI CATG 9 cut(s) 592, 691, 776, 935, 1015, 1199, 1530, 1666, 1682
FaqI GGGAC 4 cut(s) 158, 488, 813, 1641
FatI CATG 9 cut(s) 588, 687, 772, 931, 1011, 1195, 1526, 1662, 1678
Fnu4HI GCNGC 4 cut(s) 638, 976, 1540, 1543
FokI GGATG 2 cut(s) 167, 713
FriOI GRGCYC 2 cut(s) 162, 883
Fsp4HI GCNGC 4 cut(s) 638, 976, 1540, 1543
FspBI CTAG 4 cut(s) 1160, 1175, 1380, 1749
GlaI GCGC 3 cut(s) 401, 668, 1288
GluI GCNGC 4 cut(s) 638, 976, 1540, 1543
GsaI CCCAGC 1 cut(s) 582
GsuI CTGGAG 3 cut(s) 192, 806, 1186
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 6 cut(s) 46, 87, 288, 640, 930, 1661
HgaI GACGC 2 cut(s) 58, 810
HhaI GCGC 3 cut(s) 402, 669, 1289
Hin1I GRCGYC 2 cut(s) 69, 667
Hin1II CATG 9 cut(s) 592, 691, 776, 935, 1015, 1199, 1530, 1666, 1682
Hin6I GCGC 3 cut(s) 400, 667, 1287
HinP1I GCGC 3 cut(s) 400, 667, 1287
HincII GTYRAC 2 cut(s) 454, 1193
HindII GTYRAC 2 cut(s) 454, 1193
HinfI GANTC 8 cut(s) 134, 458, 629, 836, 1055, 1384, 1583, 1835
HpaI GTTAAC 1 cut(s) 454
HphI GGTGA 8 cut(s) 221, 226, 269, 439, 488, 1378, 1423, 1876
Hpy166II GTNNAC 8 cut(s) 454, 620, 763, 938, 952, 1080, 1193, 1414
Hpy188I TCNGA 9 cut(s) 368, 390, 463, 491, 523, 718, 967, 1060, 1342
Hpy8I GTNNAC 8 cut(s) 454, 620, 763, 938, 952, 1080, 1193, 1414
HpyCH4III ACNGT 2 cut(s) 831, 1522
HpyCH4IV ACGT 4 cut(s) 114, 240, 1685, 1827
HpyCH4V TGCA 4 cut(s) 653, 978, 1722, 1799
HpyF10VI GCNNNNNNNGC 8 cut(s) 52, 66, 491, 1073, 1165, 1536, 1548, 1658
HpySE526I ACGT 4 cut(s) 114, 240, 1685, 1827
Hsp92I GRCGYC 2 cut(s) 69, 667
Hsp92II CATG 9 cut(s) 592, 691, 776, 935, 1015, 1199, 1530, 1666, 1682
HspAI GCGC 3 cut(s) 400, 667, 1287
KasI GGCGCC 1 cut(s) 666
Kpn2I TCCGGA 1 cut(s) 1025
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
LmnI GCTCC 2 cut(s) 491, 878
Lsp1109I GCAGC 3 cut(s) 962, 1551, 1554
LweI GCATC 4 cut(s) 180, 557, 707, 1786
MaeI CTAG 4 cut(s) 1160, 1175, 1380, 1749
MaeII ACGT 4 cut(s) 114, 240, 1685, 1827
MaeIII GTNAC 5 cut(s) 70, 825, 1636, 1654, 1681
MalI GATC 8 cut(s) 361, 517, 543, 1173, 1182, 1298, 1788, 1821
MbiI CCGCTC 1 cut(s) 414
MboI GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 2 cut(s) 359, 1171
MhlI GDGCHC 3 cut(s) 162, 883, 1760
MluCI AATT 9 cut(s) 271, 342, 524, 648, 815, 848, 1038, 1097, 1879
Mly113I GGCGCC 1 cut(s) 667
MlyI GAGTC 2 cut(s) 143, 467
MroI TCCGGA 1 cut(s) 1025
MroXI GAANNNNTTC 2 cut(s) 384, 1145
MseI TTAA 8 cut(s) 207, 300, 408, 453, 1317, 1688, 1809, 1882
MslI CAYNNNNRTG 4 cut(s) 77, 593, 771, 1154
MspA1I CMGCKG 1 cut(s) 1652
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 3 cut(s) 785, 1204, 1400
MvnI CGCG 1 cut(s) 1289
MwoI GCNNNNNNNGC 8 cut(s) 52, 66, 491, 1073, 1165, 1536, 1548, 1658
NarI GGCGCC 1 cut(s) 667
NciI CCSGG 7 cut(s) 797, 927, 1422, 1423, 1481, 1694, 1735
NcoI CCATGG 1 cut(s) 931
NdeII GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
NlaIII CATG 9 cut(s) 592, 691, 776, 935, 1015, 1199, 1530, 1666, 1682
NlaIV GGNNCC 3 cut(s) 88, 493, 668
NmuCI GTSAC 3 cut(s) 70, 825, 1636
NspI RCATGY 2 cut(s) 1199, 1682
NspV TTCGAA 3 cut(s) 280, 846, 1347
OliI CACNNNNGTG 1 cut(s) 1154
PaeR7I CTCGAG 1 cut(s) 1546
PciI ACATGT 1 cut(s) 1195
PcsI WCGNNNNNNNCGW 1 cut(s) 1824
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 2 cut(s) 384, 1145
PfeI GAWTC 6 cut(s) 629, 836, 1055, 1384, 1583, 1835
PflMI CCANNNNNTGG 1 cut(s) 1017
PkrI GCNGC 4 cut(s) 639, 977, 1541, 1544
PleI GAGTC 2 cut(s) 142, 466
PluTI GGCGCC 1 cut(s) 670
PpsI GAGTC 2 cut(s) 142, 466
Ppu21I YACGTR 1 cut(s) 1828
PscI ACATGT 1 cut(s) 1195
Psp124BI GAGCTC 1 cut(s) 883
Psp6I CCWGG 3 cut(s) 783, 1202, 1398
PspFI CCCAGC 1 cut(s) 578
PspGI CCWGG 3 cut(s) 783, 1202, 1398
PspN4I GGNNCC 3 cut(s) 88, 493, 668
PspPI GGNCC 5 cut(s) 86, 287, 740, 763, 781
PspXI VCTCGAGB 1 cut(s) 1546
PstI CTGCAG 1 cut(s) 980
PsuI RGATCY 2 cut(s) 359, 1171
RsaI GTAC 8 cut(s) 11, 65, 833, 1005, 1081, 1508, 1713, 1830
RsaNI GTAC 8 cut(s) 10, 64, 832, 1004, 1080, 1507, 1712, 1829
RseI CAYNNNNRTG 4 cut(s) 77, 593, 771, 1154
SacI GAGCTC 1 cut(s) 883
SaqAI TTAA 8 cut(s) 207, 300, 408, 453, 1317, 1688, 1809, 1882
SatI GCNGC 4 cut(s) 638, 976, 1540, 1543
Sau3AI GATC 8 cut(s) 359, 515, 541, 1171, 1180, 1296, 1786, 1819
Sau96I GGNCC 5 cut(s) 86, 287, 740, 763, 781
ScaI AGTACT 1 cut(s) 1713
SchI GAGTC 2 cut(s) 143, 467
SduI GDGCHC 3 cut(s) 162, 883, 1760
SfaNI GCATC 4 cut(s) 180, 557, 707, 1786
SfcI CTRYAG 1 cut(s) 976
SfoI GGCGCC 1 cut(s) 668
Sfr274I CTCGAG 1 cut(s) 1546
SfuI TTCGAA 3 cut(s) 280, 846, 1347
SinI GGWCC 3 cut(s) 740, 763, 781
SlaI CTCGAG 1 cut(s) 1546
SmaI CCCGGG 1 cut(s) 1423
SmiMI CAYNNNNRTG 4 cut(s) 77, 593, 771, 1154
SmlI CTYRAG 2 cut(s) 161, 1546
SmoI CTYRAG 2 cut(s) 161, 1546
SnaBI TACGTA 1 cut(s) 1828
Sse9I AATT 9 cut(s) 271, 342, 524, 648, 815, 848, 1038, 1097, 1879
SsiI CCGC 4 cut(s) 414, 637, 1364, 1650
SspDI GGCGCC 1 cut(s) 666
SspMI CTAG 4 cut(s) 1160, 1175, 1380, 1749
SstI GAGCTC 1 cut(s) 883
StyI CCWWGG 4 cut(s) 670, 743, 931, 1748
TaaI ACNGT 2 cut(s) 831, 1522
TaiI ACGT 4 cut(s) 117, 243, 1688, 1830
TaqI TCGA 7 cut(s) 132, 137, 280, 678, 846, 1347, 1547
TasI AATT 9 cut(s) 271, 342, 524, 648, 815, 848, 1038, 1097, 1879
TatI WGTACW 1 cut(s) 1711
TauI GCSGC 1 cut(s) 640
TfiI GAWTC 6 cut(s) 629, 836, 1055, 1384, 1583, 1835
Tru1I TTAA 8 cut(s) 207, 300, 408, 453, 1317, 1688, 1809, 1882
Tru9I TTAA 8 cut(s) 207, 300, 408, 453, 1317, 1688, 1809, 1882
TscAI CASTG 4 cut(s) 259, 1225, 1641, 1806
TseFI GTSAC 3 cut(s) 70, 825, 1636
TseI GCWGC 3 cut(s) 975, 1539, 1542
Tsp45I GTSAC 3 cut(s) 70, 825, 1636
TspDTI ATGAA 5 cut(s) 715, 886, 1110, 1251, 1479
TspMI CCCGGG 1 cut(s) 1421
TspRI CASTG 4 cut(s) 259, 1225, 1641, 1806
Van91I CCANNNNNTGG 1 cut(s) 1017
VpaK11BI GGWCC 3 cut(s) 740, 763, 781
XapI RAATTY 4 cut(s) 271, 524, 815, 848
XbaI TCTAGA 1 cut(s) 1174
XceI RCATGY 2 cut(s) 1199, 1682
XcmI CCANNNNNNNNNTGG 1 cut(s) 1134
XhoI CTCGAG 1 cut(s) 1546
XmaI CCCGGG 1 cut(s) 1421
XmaJI CCTAGG 1 cut(s) 1748
XmnI GAANNNNTTC 2 cut(s) 384, 1145
XspI CTAG 4 cut(s) 1160, 1175, 1380, 1749
ZrmI AGTACT 1 cut(s) 1713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.