Rw2G006620

Domain of unknown function (DUF4413)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001193
Physical Location & Seq
Forward (+)
6388765 .. 6389725
961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006620.1

Sequence Viewer

Length: 792 bp
ATGGTGATGCTGGAACGATGGAGAATCAATCGCCTCCAATTCCTATCACATCAACAGAGAATTCGACCATTGCTGCACCACCAACTGAGAACCAGGAGAACTCAACACCAACAGGGTACGAATGAGTTGACTAATGTAAACCGCCCAGGGTTTATGAGAGCACCAAAGTCGGTTGTATGGGATCATTTTGAAAAACAGGTAATTGGTGGTAAAAGAAAAGCTGTGTGCAACCATTGTAAGAAGCAATTGATTTCTGAAAAGAATAGTGGAACTACACACTTACATGATCATATTAAGTCTTGTCTTTACAAGAAACAAAGAACCATAGATCTGTCAATGTTACATCCTACAAAATCTAGTGATGGGTCTACTAAACTGGGCACTTATAGCTTTAACCCAGACAATGCCAGGAAGGAACTTGGACATATGATAATTCTTCATGAGTATCCGTTGTCAATTGTGGATCATGTTGGATTCAGAAGGTATTCTTTTGAACTGCAACCGTTGTTCCTAGTTCCTACTCGAAACACAATCAAGAAAGATATATTTAAGATTTTTGAAGTTGAAAGGGAGAAAACAATGAAGTTGTTGGATTCGAATAGAAGCAGAATTGCTATTACAACGGATATGTGGACATCAAGTAATCAAAAGAGAGGATTTATGACAATCACTTCCCACTTCATTGATGATTCGTGGAATTTGCAAAATCGACTCTTAAGGTTTATTTTTGCTAGGTTTATATATATATACAGCGGAGGTACTTGCAGATGCTTTGGTGAATTGCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4413 Dimer_Tnp_hAT zf-BED

Protein Analysis

263

Amino Acids

31.09

Weight (kDa)

10.17

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-BED PF02892 57 - 101 6.3e-06 BED zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 368
AciI CCGC 2 cut(s) 142, 753
AclWI GGATC 2 cut(s) 189, 471
AcsI RAATTY 2 cut(s) 60, 697
AfaI GTAC 2 cut(s) 118, 760
AflII CTTAAG 1 cut(s) 715
AgsI TTSAA 4 cut(s) 191, 494, 560, 566
AjnI CCWGG 3 cut(s) 92, 145, 407
AluBI AGCT 2 cut(s) 221, 390
AluI AGCT 2 cut(s) 221, 390
Alw21I GWGCWC 1 cut(s) 163
AlwI GGATC 2 cut(s) 189, 471
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 2 cut(s) 60, 697
Asp700I GAANNNNTTC 1 cut(s) 484
AsuHPI GGTGA 2 cut(s) 16, 788
AsuII TTCGAA 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 12, 356
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BciT130I CCWGG 3 cut(s) 94, 147, 409
BciVI GTATCC 1 cut(s) 456
BclI TGATCA 1 cut(s) 286
BfaI CTAG 3 cut(s) 357, 512, 732
BfrI CTTAAG 1 cut(s) 715
BfuI GTATCC 1 cut(s) 456
BglII AGATCT 1 cut(s) 328
BisI GCNGC 1 cut(s) 74
BlsI GCNGC 1 cut(s) 75
Bme1390I CCNGG 3 cut(s) 94, 147, 409
BmrFI CCNGG 3 cut(s) 94, 147, 409
BmrI ACTGGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 758
BmuI ACTGGG 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 596
BsaJI CCNNGG 2 cut(s) 145, 146
Bse1I ACTGG 1 cut(s) 381
Bse3DI GCAATG 1 cut(s) 68
BseBI CCWGG 3 cut(s) 94, 147, 409
BseDI CCNNGG 2 cut(s) 145, 146
BseGI GGATG 1 cut(s) 343
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 381
BseSI GKGCMC 1 cut(s) 383
BseXI GCAGC 1 cut(s) 60
BsgI GTGCAG 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 163
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 2 cut(s) 163, 383
Bsp143I GATC 4 cut(s) 181, 286, 328, 463
BspACI CCGC 2 cut(s) 142, 753
BspCNI CTCAG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 439
BspPI GGATC 2 cut(s) 189, 471
BspT104I TTCGAA 1 cut(s) 596
BspTI CTTAAG 1 cut(s) 715
BsrDI GCAATG 1 cut(s) 68
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 145, 146
BssMI GATC 4 cut(s) 181, 286, 328, 463
Bst2UI CCWGG 3 cut(s) 94, 147, 409
Bst4CI ACNGT 1 cut(s) 504
BstAFI CTTAAG 1 cut(s) 715
BstBI TTCGAA 1 cut(s) 596
BstDEI CTNAG 1 cut(s) 86
BstF5I GGATG 1 cut(s) 343
BstKTI GATC 4 cut(s) 184, 289, 331, 466
BstMBI GATC 4 cut(s) 181, 286, 328, 463
BstMWI GCNNNNNNNGC 1 cut(s) 387
BstNI CCWGG 3 cut(s) 94, 147, 409
BstSCI CCNGG 3 cut(s) 92, 145, 407
BstSLI GKGCMC 1 cut(s) 383
BstV1I GCAGC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuI GTATCC 1 cut(s) 456
BtsCI GGATG 1 cut(s) 343
CciI TCATGA 1 cut(s) 439
Csp6I GTAC 2 cut(s) 117, 759
CviAII CATG 3 cut(s) 284, 440, 467
CviJI RGCY 2 cut(s) 221, 390
CviKI_1 RGCY 2 cut(s) 221, 390
CviQI GTAC 2 cut(s) 117, 759
DdeI CTNAG 1 cut(s) 86
DpnI GATC 4 cut(s) 183, 288, 330, 465
DpnII GATC 4 cut(s) 181, 286, 328, 463
EcoRI GAATTC 1 cut(s) 60
EcoRII CCWGG 3 cut(s) 92, 145, 407
FaeI CATG 3 cut(s) 287, 443, 470
FalI AAGNNNNNCTT 2 cut(s) 472, 504
FatI CATG 3 cut(s) 283, 439, 466
FauNDI CATATG 1 cut(s) 426
FbaI TGATCA 1 cut(s) 286
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 74
FokI GGATG 1 cut(s) 330
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 3 cut(s) 357, 512, 732
GluI GCNGC 1 cut(s) 74
Hin1II CATG 3 cut(s) 287, 443, 470
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 5 cut(s) 24, 474, 593, 689, 711
HphI GGTGA 2 cut(s) 16, 788
Hpy166II GTNNAC 4 cut(s) 129, 139, 369, 633
Hpy188I TCNGA 2 cut(s) 256, 479
Hpy188III TCNNGA 2 cut(s) 440, 535
Hpy8I GTNNAC 4 cut(s) 129, 139, 369, 633
HpyAV CCTTC 2 cut(s) 406, 474
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4V TGCA 5 cut(s) 76, 228, 499, 703, 765
HpyF10VI GCNNNNNNNGC 1 cut(s) 387
HpyF3I CTNAG 1 cut(s) 86
Hsp92II CATG 3 cut(s) 287, 443, 470
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 4 cut(s) 181, 286, 328, 463
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 1 cut(s) 758
MaeI CTAG 3 cut(s) 357, 512, 732
MaeIII GTNAC 1 cut(s) 339
MalI GATC 4 cut(s) 183, 288, 330, 465
MboI GATC 4 cut(s) 181, 286, 328, 463
MboII GAAGA 1 cut(s) 428
MfeI CAATTG 2 cut(s) 245, 456
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 163, 383
MluCI AATT 9 cut(s) 38, 60, 201, 245, 432, 456, 609, 697, 779
MlyI GAGTC 1 cut(s) 705
MmeI TCCRAC 2 cut(s) 451, 570
MnlI CCTC 3 cut(s) 44, 647, 749
MroXI GAANNNNTTC 1 cut(s) 484
MseI TTAA 4 cut(s) 294, 393, 549, 716
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 753
MspCI CTTAAG 1 cut(s) 715
MspR9I CCNGG 3 cut(s) 94, 147, 409
MunI CAATTG 2 cut(s) 245, 456
MvaI CCWGG 3 cut(s) 94, 147, 409
MwoI GCNNNNNNNGC 1 cut(s) 387
NdeI CATATG 1 cut(s) 426
NdeII GATC 4 cut(s) 181, 286, 328, 463
NlaIII CATG 3 cut(s) 287, 443, 470
NspV TTCGAA 1 cut(s) 596
PagI TCATGA 1 cut(s) 439
PasI CCCWGGG 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 4 cut(s) 24, 474, 593, 689
PkrI GCNGC 1 cut(s) 75
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
Psp6I CCWGG 3 cut(s) 92, 145, 407
PspGI CCWGG 3 cut(s) 92, 145, 407
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 2 cut(s) 118, 760
RsaNI GTAC 2 cut(s) 117, 759
RseI CAYNNNNRTG 1 cut(s) 282
SaqAI TTAA 4 cut(s) 294, 393, 549, 716
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 4 cut(s) 181, 286, 328, 463
SchI GAGTC 1 cut(s) 705
ScrFI CCNGG 3 cut(s) 94, 147, 409
SduI GDGCHC 2 cut(s) 163, 383
SetI ASST 7 cut(s) 201, 223, 392, 485, 722, 737, 760
SfaNI GCATC 1 cut(s) 758
SfuI TTCGAA 1 cut(s) 596
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 715
SmoI CTYRAG 1 cut(s) 715
Sse9I AATT 9 cut(s) 38, 60, 201, 245, 432, 456, 609, 697, 779
SsiI CCGC 2 cut(s) 142, 753
SspMI CTAG 3 cut(s) 357, 512, 732
StyD4I CCNGG 3 cut(s) 92, 145, 407
TaaI ACNGT 1 cut(s) 504
TaqI TCGA 4 cut(s) 64, 523, 596, 709
TasI AATT 9 cut(s) 38, 60, 201, 245, 432, 456, 609, 697, 779
TfiI GAWTC 4 cut(s) 24, 474, 593, 689
Tru1I TTAA 4 cut(s) 294, 393, 549, 716
Tru9I TTAA 4 cut(s) 294, 393, 549, 716
TseI GCWGC 1 cut(s) 73
TspDTI ATGAA 3 cut(s) 428, 596, 670
TspGWI ACGGA 2 cut(s) 438, 638
Vha464I CTTAAG 1 cut(s) 715
XapI RAATTY 2 cut(s) 60, 697
XmiI GTMKAC 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 3 cut(s) 357, 512, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.