FvH4_3g11044

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000956
Physical Location & Seq
Reverse (-)
6519218 .. 6520939
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11044.t1

Sequence Viewer

Length: 1590 bp
ATGAAGGTTCTAGTGCTCGTTATTCTATCAGTCCTTTCGTTTTCAGTACCATGTACAACTTCAGATCCAATTCGCACAACTGACTTCCTCCACTGCCTTACAAATCATTCTCACTACTCCACCTCCCCAATCTCTGAAGCCATTTACACGTACCAAAACACCTCTTTTCAACCTGCTCTCTTGGCATACATTAGAAACCGCAGATATTCCACACCCACAACACCGAAACCTTTGGCGATTGTAGCAGCCAAGCATGAATCCCATGTCCAATCGACTGTCATTTGTGCACAACAACATGGATTTCAAATAAGAATCCGAAGCGGTGGCCATGATTTTGAGGGTCTATCGTACGTATCAAGTGTCACACCCTTTGTCATGCTTGACATGTCTAATCTTAGATCCATCGATATTAATGTCACGGACGAGAGTGCTTGGGTAGAATCTGGGGCTACTCTTGGTGAGCTTTATTTCAATATTGGAAACAAAAGCAATGTGCATGGATTTCCTGCTGGAGTTTGCCCCACTGTTGGTATTGGTGGCCATCTTAGTGGTGGTGGTTATGGCCCTTTGATGAGAAAATATGGACTTACAGTCGATAACGTTGTAGACGCTAAGCTAGTCAATGCGAACGGTACAATGCTTGATAGAAAATCAATGGGAGAAGATCTTTTCTGGGCCATTAGAGGTGGTGGAGCAAGCTTTGGAGTCGTTGTGTCTTGGAAGATCAAATTGGTTCCAGTTCCAACCAGAGTGACTGTATTCAATGTTTTAAGGACTTTGGAACAGGGCGCTATCGATACTGTTCATCGGTGGCAATACGTAGCGCCTAAGCTGCCTAAAGAGATCTTCATTAGGGCAATGCTTCAAGTGAAGCAAAATAGTACTTCAGGTAAGTTGGCTATGCAAGTGTCATTCATTTGTCATTATTTGGGAGATAGTGAGAAACTTCTTTCTTTGATGAATCAGAGTTTTCCTGAATTGGGTTTGCAGCAAAGTGATTGCTTTGAAATGAGTTGGGTAGAATCCACTGTGTTCTGGGGTAACCACCCGATTGGAACTCCCGCAACTGTTCTGCTTGATAGGCCAATGGGGCCGCCAGATTTCTTCAAGGGCAAGTCAGACTATGTGAAAGAGCCGATTCCGAAACATGGAATAGAATCGATATTGGACTTGATGTTAAAGCCGGGTCTGGATAAACTGTACATGGAATGGAACCCTTATGGTGGAAGAATGAGTCAGATTTCGGAGTCGGAAACTCCATTCCCTCACAGGGAAGGAAACCTGTTCTTGATTCAGTATCTGGGATATTGGAAAGAAGACAAGGCTGAGACGGCACACCGCTACCTTGACTTGATTAGCAAAATGTATCAAGAAATGACTCCATTTGTGTCCAAGAACCCAAGAGAGGCCTTCCTAAACTATAGGGATCTTGACCTTGGTGCCAATACGGGTAATCAGACTAATGTTGAAACTGCTAGGAATTATGGAAGCATGTATTTCAAAGAAAACTTTGATAGACTGGTCCGTGTGAAAACTGCGGTTGATCCTTGGAATTTTTTCAAGAATGAACAGAGTATCCCGCCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

530

Amino Acids

59.34

Weight (kDa)

7.25

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 77 - 214 3.7e-27 FAD binding domain
BBE PF08031 470 - 527 1.6e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 1439
AccI GTMKAC 1 cut(s) 606
AciI CCGC 7 cut(s) 199, 321, 1062, 1094, 1339, 1538, 1580
AclI AACGTT 1 cut(s) 600
AclWI GGATC 4 cut(s) 59, 393, 1434, 1538
AcoI YGGCCR 2 cut(s) 325, 538
AcsI RAATTY 1 cut(s) 1552
AcuI CTGAAG 3 cut(s) 45, 156, 870
AfaI GTAC 7 cut(s) 48, 55, 152, 350, 634, 883, 1202
AfiI CCNNNNNNNGG 6 cut(s) 527, 980, 1148, 1223, 1270, 1405
AflIII ACRYGT 2 cut(s) 147, 384
AhdI GACNNNNNGTC 1 cut(s) 590
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AloI GAACNNNNNNTCC 1 cut(s) 1560
AluBI AGCT 4 cut(s) 463, 616, 699, 832
AluI AGCT 4 cut(s) 463, 616, 699, 832
Alw21I GWGCWC 2 cut(s) 18, 289
Alw26I GTCTC 1 cut(s) 1322
Alw44I GTGCAC 1 cut(s) 285
AlwI GGATC 4 cut(s) 59, 393, 1434, 1538
AlwNI CAGNNNCTG 1 cut(s) 1300
AoxI GGCC 7 cut(s) 325, 538, 562, 675, 1082, 1091, 1407
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 3 cut(s) 245, 832, 988
ApoI RAATTY 1 cut(s) 1552
AseI ATTAAT 1 cut(s) 411
Asp700I GAANNNNTTC 1 cut(s) 1556
AspLEI GCGC 2 cut(s) 791, 826
AspS9I GGNCC 4 cut(s) 563, 675, 1091, 1522
AsuC2I CCSGG 1 cut(s) 1185
AsuHPI GGTGA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 1522
BaeGI GKGCMC 1 cut(s) 289
BalI TGGCCA 2 cut(s) 327, 540
BanI GGYRCC 1 cut(s) 1439
BbsI GAAGAC 1 cut(s) 1323
Bbv12I GWGCWC 2 cut(s) 18, 289
BbvI GCAGC 3 cut(s) 257, 819, 1000
BccI CCATC 2 cut(s) 410, 549
BceAI ACGGC 1 cut(s) 1347
BciVI GTATCC 1 cut(s) 1586
BcnI CCSGG 1 cut(s) 1185
BcoDI GTCTC 1 cut(s) 1322
BfaI CTAG 3 cut(s) 11, 617, 1476
BfmI CTRYAG 1 cut(s) 1420
BfoI RGCGCY 2 cut(s) 792, 827
BfuAI ACCTGC 1 cut(s) 181
BfuI GTATCC 1 cut(s) 1586
BglI GCCNNNNNGGC 1 cut(s) 1090
BglII AGATCT 2 cut(s) 664, 843
BisI GCNGC 4 cut(s) 246, 833, 989, 1094
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 4 cut(s) 247, 834, 990, 1095
BmcAI AGTACT 1 cut(s) 883
Bme1390I CCNGG 1 cut(s) 1185
Bme18I GGWCC 1 cut(s) 1522
BmeRI GACNNNNNGTC 1 cut(s) 590
BmgT120I GGNCC 4 cut(s) 563, 675, 1091, 1522
BmiI GGNNCC 4 cut(s) 735, 1092, 1214, 1441
BmrFI CCNGG 1 cut(s) 1185
BpiI GAAGAC 1 cut(s) 1323
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 828
Bpu1102I GCTNAGC 1 cut(s) 612
BpuMI CCSGG 1 cut(s) 1185
Bsa29I ATCGAT 3 cut(s) 405, 795, 1160
BsaAI YACGTR 3 cut(s) 150, 352, 820
BsaJI CCNNGG 2 cut(s) 1435, 1547
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 696, 726
Bsc4I CCNNNNNNNGG 6 cut(s) 527, 980, 1148, 1223, 1270, 1405
Bse1I ACTGG 2 cut(s) 737, 1524
Bse3DI GCAATG 2 cut(s) 496, 864
BseCI ATCGAT 3 cut(s) 405, 795, 1160
BseDI CCNNGG 2 cut(s) 1435, 1547
BseLI CCNNNNNNNGG 6 cut(s) 527, 980, 1148, 1223, 1270, 1405
BseMI GCAATG 2 cut(s) 496, 864
BseMII CTCAG 1 cut(s) 1317
BseNI ACTGG 2 cut(s) 737, 1524
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 3 cut(s) 257, 819, 1000
BshFI GGCC 7 cut(s) 327, 540, 564, 677, 1084, 1093, 1409
BshNI GGYRCC 1 cut(s) 1439
BshVI ATCGAT 3 cut(s) 405, 795, 1160
BsiHKAI GWGCWC 2 cut(s) 18, 289
BsiSI CCGG 1 cut(s) 1184
BsiWI CGTACG 1 cut(s) 348
BslI CCNNNNNNNGG 6 cut(s) 527, 980, 1148, 1223, 1270, 1405
BsmAI GTCTC 1 cut(s) 1322
BsmBI CGTCTC 1 cut(s) 1322
BsnI GGCC 7 cut(s) 327, 540, 564, 677, 1084, 1093, 1409
Bsp1286I GDGCHC 2 cut(s) 18, 289
Bsp1407I TGTACA 2 cut(s) 53, 1200
Bsp143I GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
Bsp1720I GCTNAGC 1 cut(s) 612
BspACI CCGC 7 cut(s) 199, 321, 1062, 1094, 1339, 1538, 1580
BspANI GGCC 7 cut(s) 327, 540, 564, 677, 1084, 1093, 1409
BspCNI CTCAG 1 cut(s) 1318
BspDI ATCGAT 3 cut(s) 405, 795, 1160
BspLI GGNNCC 4 cut(s) 735, 1092, 1214, 1441
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 4 cut(s) 59, 393, 1434, 1538
BspT107I GGYRCC 1 cut(s) 1439
BsrDI GCAATG 2 cut(s) 496, 864
BsrGI TGTACA 2 cut(s) 53, 1200
BsrI ACTGG 2 cut(s) 737, 1524
BssECI CCNNGG 2 cut(s) 1435, 1547
BssMI GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
BssT1I CCWWGG 2 cut(s) 1435, 1547
Bst4CI ACNGT 9 cut(s) 277, 526, 592, 632, 757, 802, 1030, 1069, 1200
BstAUI TGTACA 2 cut(s) 53, 1200
BstBAI YACGTR 3 cut(s) 150, 352, 820
BstC8I GCNNGC 1 cut(s) 697
BstDEI CTNAG 5 cut(s) 395, 545, 612, 828, 1326
BstEII GGTNACC 1 cut(s) 1040
BstH2I RGCGCY 2 cut(s) 792, 827
BstHHI GCGC 2 cut(s) 791, 826
BstKTI GATC 7 cut(s) 67, 401, 667, 726, 846, 1429, 1546
BstMAI GTCTC 1 cut(s) 1322
BstMBI GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
BstMWI GCNNNNNNNGC 6 cut(s) 182, 242, 832, 1081, 1090, 1331
BstNSI RCATGY 2 cut(s) 388, 1495
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 1 cut(s) 1183
BstSFI CTRYAG 1 cut(s) 1420
BstSLI GKGCMC 1 cut(s) 289
BstSNI TACGTA 2 cut(s) 352, 820
BstV1I GCAGC 3 cut(s) 257, 819, 1000
BstV2I GAAGAC 1 cut(s) 1323
BstX2I RGATCY 5 cut(s) 64, 398, 664, 843, 1426
BstXI CCANNNNNNTGG 2 cut(s) 548, 1052
BstYI RGATCY 5 cut(s) 64, 398, 664, 843, 1426
Bsu15I ATCGAT 3 cut(s) 405, 795, 1160
BsuI GTATCC 1 cut(s) 1586
BsuRI GGCC 7 cut(s) 327, 540, 564, 677, 1084, 1093, 1409
BsuTUI ATCGAT 3 cut(s) 405, 795, 1160
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 3 cut(s) 91, 522, 1026
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 697
CaiI CAGNNNCTG 1 cut(s) 1300
CfoI GCGC 2 cut(s) 791, 826
Cfr13I GGNCC 4 cut(s) 563, 675, 1091, 1522
ClaI ATCGAT 3 cut(s) 405, 795, 1160
CseI GACGC 1 cut(s) 617
Csp6I GTAC 7 cut(s) 47, 54, 151, 349, 633, 882, 1201
CviQI GTAC 7 cut(s) 47, 54, 151, 349, 633, 882, 1201
DdeI CTNAG 5 cut(s) 395, 545, 612, 828, 1326
DpnI GATC 7 cut(s) 66, 400, 666, 725, 845, 1428, 1545
DpnII GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
DriI GACNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 2 cut(s) 325, 538
Eam1105I GACNNNNNGTC 1 cut(s) 590
Eco105I TACGTA 2 cut(s) 352, 820
Eco130I CCWWGG 2 cut(s) 1435, 1547
Eco147I AGGCCT 1 cut(s) 1409
Eco47I GGWCC 1 cut(s) 1522
Eco57I CTGAAG 3 cut(s) 45, 156, 870
Eco91I GGTNACC 1 cut(s) 1040
EcoO65I GGTNACC 1 cut(s) 1040
EcoT14I CCWWGG 2 cut(s) 1435, 1547
ErhI CCWWGG 2 cut(s) 1435, 1547
Esp3I CGTCTC 1 cut(s) 1322
FauI CCCGC 2 cut(s) 1069, 1587
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 4 cut(s) 246, 833, 989, 1094
Fsp4HI GCNGC 4 cut(s) 246, 833, 989, 1094
FspBI CTAG 3 cut(s) 11, 617, 1476
GlaI GCGC 2 cut(s) 790, 825
GluI GCNGC 4 cut(s) 246, 833, 989, 1094
GsuI CTGGAG 1 cut(s) 531
HaeII RGCGCY 2 cut(s) 792, 827
HaeIII GGCC 7 cut(s) 327, 540, 564, 677, 1084, 1093, 1409
HapII CCGG 1 cut(s) 1184
HgaI GACGC 1 cut(s) 617
HhaI GCGC 2 cut(s) 791, 826
Hin6I GCGC 2 cut(s) 789, 824
HinP1I GCGC 2 cut(s) 789, 824
HindIII AAGCTT 1 cut(s) 697
HpaII CCGG 1 cut(s) 1184
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 2 cut(s) 287, 607
Hpy188III TCNNGA 6 cut(s) 974, 1190, 1288, 1370, 1430, 1561
Hpy8I GTNNAC 2 cut(s) 287, 607
HpyAV CCTTC 2 cut(s) 1268, 1420
HpyCH4III ACNGT 9 cut(s) 277, 526, 592, 632, 757, 802, 1030, 1069, 1200
HpyCH4IV ACGT 4 cut(s) 149, 351, 600, 819
HpyCH4V TGCA 4 cut(s) 287, 496, 904, 988
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 242, 832, 1081, 1090, 1331
HpyF3I CTNAG 5 cut(s) 395, 545, 612, 828, 1326
HpySE526I ACGT 4 cut(s) 149, 351, 600, 819
HspAI GCGC 2 cut(s) 789, 824
Kzo9I GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 3 cut(s) 257, 819, 1000
MaeI CTAG 3 cut(s) 11, 617, 1476
MaeII ACGT 4 cut(s) 149, 351, 600, 819
MaeIII GTNAC 4 cut(s) 361, 415, 751, 1040
MalI GATC 7 cut(s) 66, 400, 666, 725, 845, 1428, 1545
MboI GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
MboII GAAGA 6 cut(s) 674, 733, 838, 1096, 1239, 1328
MflI RGATCY 5 cut(s) 64, 398, 664, 843, 1426
MhlI GDGCHC 2 cut(s) 18, 289
MlsI TGGCCA 2 cut(s) 327, 540
MluCI AATT 5 cut(s) 69, 728, 977, 1480, 1552
MluNI TGGCCA 2 cut(s) 327, 540
MlyI GAGTC 4 cut(s) 714, 1243, 1256, 1372
MmeI TCCRAC 2 cut(s) 767, 1230
MnlI CCTC 7 cut(s) 98, 133, 172, 331, 677, 1275, 1399
Mox20I TGGCCA 2 cut(s) 327, 540
MroXI GAANNNNTTC 1 cut(s) 1556
MscI TGGCCA 2 cut(s) 327, 540
MseI TTAA 3 cut(s) 411, 770, 1178
MslI CAYNNNNRTG 1 cut(s) 546
Msp20I TGGCCA 2 cut(s) 327, 540
MspI CCGG 1 cut(s) 1184
MspR9I CCNGG 1 cut(s) 1185
MwoI GCNNNNNNNGC 6 cut(s) 182, 242, 832, 1081, 1090, 1331
NciI CCSGG 1 cut(s) 1185
NdeII GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
NlaIV GGNNCC 4 cut(s) 735, 1092, 1214, 1441
NmuCI GTSAC 3 cut(s) 361, 415, 751
NspI RCATGY 2 cut(s) 388, 1495
PceI AGGCCT 1 cut(s) 1409
PciI ACATGT 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 1556
PfeI GAWTC 8 cut(s) 257, 312, 440, 961, 1022, 1138, 1157, 1291
Pfl23II CGTACG 1 cut(s) 348
PkrI GCNGC 4 cut(s) 247, 834, 990, 1095
PleI GAGTC 4 cut(s) 713, 1242, 1255, 1372
PpsI GAGTC 4 cut(s) 713, 1242, 1255, 1372
Ppu21I YACGTR 3 cut(s) 150, 352, 820
PscI ACATGT 1 cut(s) 384
PshBI ATTAAT 1 cut(s) 411
Psp1406I AACGTT 1 cut(s) 600
PspEI GGTNACC 1 cut(s) 1040
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 4 cut(s) 735, 1092, 1214, 1441
PspPI GGNCC 4 cut(s) 563, 675, 1091, 1522
PstNI CAGNNNCTG 1 cut(s) 1300
PsuI RGATCY 5 cut(s) 64, 398, 664, 843, 1426
RsaI GTAC 7 cut(s) 48, 55, 152, 350, 634, 883, 1202
RsaNI GTAC 7 cut(s) 47, 54, 151, 349, 633, 882, 1201
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 3 cut(s) 411, 770, 1178
SatI GCNGC 4 cut(s) 246, 833, 989, 1094
Sau3AI GATC 7 cut(s) 64, 398, 664, 723, 843, 1426, 1543
Sau96I GGNCC 4 cut(s) 563, 675, 1091, 1522
ScaI AGTACT 1 cut(s) 883
SchI GAGTC 4 cut(s) 714, 1243, 1256, 1372
ScrFI CCNGG 1 cut(s) 1185
SduI GDGCHC 2 cut(s) 18, 289
SfcI CTRYAG 1 cut(s) 1420
SfiI GGCCNNNNNGGCC 1 cut(s) 1090
SinI GGWCC 1 cut(s) 1522
SmiMI CAYNNNNRTG 1 cut(s) 546
SnaBI TACGTA 2 cut(s) 352, 820
Sse9I AATT 5 cut(s) 69, 728, 977, 1480, 1552
SseBI AGGCCT 1 cut(s) 1409
SsiI CCGC 7 cut(s) 199, 321, 1062, 1094, 1339, 1538, 1580
SspI AATATT 1 cut(s) 475
SspMI CTAG 3 cut(s) 11, 617, 1476
StuI AGGCCT 1 cut(s) 1409
StyD4I CCNGG 1 cut(s) 1183
StyI CCWWGG 2 cut(s) 1435, 1547
TaaI ACNGT 9 cut(s) 277, 526, 592, 632, 757, 802, 1030, 1069, 1200
TaiI ACGT 4 cut(s) 152, 354, 603, 822
TaqI TCGA 5 cut(s) 272, 405, 594, 795, 1160
TasI AATT 5 cut(s) 69, 728, 977, 1480, 1552
TatI WGTACW 3 cut(s) 53, 881, 1200
TauI GCSGC 1 cut(s) 1096
TfiI GAWTC 8 cut(s) 257, 312, 440, 961, 1022, 1138, 1157, 1291
Tru1I TTAA 3 cut(s) 411, 770, 1178
Tru9I TTAA 3 cut(s) 411, 770, 1178
TscAI CASTG 3 cut(s) 98, 529, 1033
TseFI GTSAC 3 cut(s) 361, 415, 751
TseI GCWGC 3 cut(s) 245, 832, 988
Tsp45I GTSAC 3 cut(s) 361, 415, 751
TspDTI ATGAA 7 cut(s) 17, 270, 794, 838, 904, 974, 1581
TspGWI ACGGA 2 cut(s) 434, 1514
TspRI CASTG 3 cut(s) 98, 529, 1033
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 1522
VspI ATTAAT 1 cut(s) 411
XapI RAATTY 1 cut(s) 1552
XceI RCATGY 2 cut(s) 388, 1495
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 1556
XspI CTAG 3 cut(s) 11, 617, 1476
ZrmI AGTACT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.