Prupe.1G032800_v2.0.a1

Berberine and berberine like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000956
Physical Location & Seq
Forward (+)
2282973 .. 2284827
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G032800.1

Sequence Viewer

Length: 1593 bp
ATGAAGTTCAGAAACCCTCCAGTCCTGCTTTTCATTCTCTCTGTCCTTTCACTCTCGATACCATGGACAACTGCAGGAACAGTTTCCATTGATCGCTTTCTCCAATGCCTCACAAAATATCCTCACCCTGCCCACACAATCCAAGAATCAATTTACACCCCTCAAAATAGCTCATTTCAATCTGTTTTGGTAGCACATATTAATAACCGCAGATATTCTACAGCTGCAACTCCAAAACCTCTGGCCATAATAGCGGCCAAGAATGAGTCCCATGTCCAAGCAACTGTGCTTTGTGCCAAACATCATGGGTTGCAAATTAAAATCCGAAGTGGTGGCCATGATTTCGAGGGTTTATCTTACACGTCGGATGTGCCCTTTGTCATTCTCGACATGTTTAACATTAACTCCATTGATGTTAATGTTGCTGATAAGAGCGCTTGGGTTCACTCTGGAGCTACTCTTGGAGAAGTTTATTATGCAATTGGTACAAAAACCAACGTTTACGGCTTTCCTGCTGGGATTTGTCCAACAGTTGGTGCTGGTGGCCATTTTAGTGGCGGCGGCTATGGGTTTTTGATGAGAAAGTATGGGCTTAGCGTCGATAATATCGTGGATGCTAGGCTAGTTACTGCCAATGGTAGAATCCTTGATAGAAAGTCAATGGGAGAGGACCTTTTCTGGGCCATCAGAGGAGGTGGTGGAGCTAGCTTCGGAGTTATTCTTTCATGGAAGCTCAAGCTGGTTCCAGTTCCACCCAAAGTGACTGTGTTCAACCTTACAAAGACCATAGAGGAAGGTGTTACAGATTTGGTTTACAAGTGGCAAACTCTTGCACCCCAGCTTCCCAAAGATGTCTTCCTCAGAGCACAGCCACAAGTGAAGAATATTGATACCAAGGGAAACAAGACTGTGGGAGTATCATTCATCGGCCATTTCTTGGGAACAAGTGACAAGGTTGTGGCATTGTTGAATGAGAGTTTCCCTGAACTGGGCCTGCAACGAAAAGACTGCTATGAAGTGAGCTGGGTGGAATCCACTGTATTTTGGGCTGAAAGTCCCATTGGAACCCCCATAGAAATTCTTCTCAGCAAGCCAACTGAACCAGAGACCTTCTACAAAGGTAAGTCAGACTATGTGAAGGAACCAATTCCTAAACATGTTTTTGATTCCATATGGAAGAAGATGATTGAGATAGAGCACATTTGGTTGGATTGGAACCCTTACGGTGGAAGAATGAGTGAGATTTCTGAGTCAGCAACTCCATACCCTCACAGGGCCGGAAACCTATTTTTTGCTCTGTACTATTCGTCGTGGTATGACGAAGGGATTGAGGCCACCAACAAGTACGTTCGCTTAACAAGAGAGTTGTATGACATGATGACACCGTATGTCTCCAAGAATCCAAGAGAGGCGTTCCAAAACTACAGGGATCTCGACATTGGTGCGAACCAGGATAATAAGACTACCTTTGAAACTGCTACACTTTATGGAAGGAAGTACTTCAAAGGTAATTTTGATAGATTGGTGCGTGTGAAAACCATGGTCGATCCTCATAATTTTTTCAAGCATAAGCAAAGTATCCCTCCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

531

Amino Acids

59.52

Weight (kDa)

8.89

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 533, 937
AciI CCGC 4 cut(s) 208, 254, 558, 561
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 1437, 1541
AcoI YGGCCR 5 cut(s) 243, 255, 334, 544, 928
AcsI RAATTY 1 cut(s) 1077
AfaI GTAC 4 cut(s) 487, 1301, 1346, 1499
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 7 cut(s) 533, 679, 937, 988, 989, 1226, 1273
AflIII ACRYGT 3 cut(s) 360, 390, 1156
AgsI TTSAA 6 cut(s) 179, 772, 970, 1472, 1504, 1564
AjiI CACGTC 1 cut(s) 363
AjnI CCWGG 1 cut(s) 1449
AjuI GAANNNNNNNTTGG 4 cut(s) 839, 871, 1044, 1076
AluBI AGCT 9 cut(s) 171, 224, 455, 704, 708, 733, 739, 841, 1023
AluI AGCT 9 cut(s) 171, 224, 455, 704, 708, 733, 739, 841, 1023
Alw21I GWGCWC 2 cut(s) 868, 1200
Alw26I GTCTC 2 cut(s) 1100, 1396
AlwI GGATC 2 cut(s) 1437, 1541
Aor51HI AGCGCT 1 cut(s) 436
AoxI GGCC 9 cut(s) 243, 255, 334, 544, 681, 928, 991, 1275, 1332
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 1 cut(s) 1077
AseI ATTAAT 1 cut(s) 201
Asp700I GAANNNNTTC 4 cut(s) 82, 1080, 1146, 1499
AspLEI GCGC 1 cut(s) 437
AspS9I GGNCC 4 cut(s) 670, 681, 991, 1275
AsuHPI GGTGA 1 cut(s) 116
AsuNHI GCTAGC 1 cut(s) 704
AvaII GGWCC 1 cut(s) 670
BaeGI GKGCMC 1 cut(s) 375
BalI TGGCCA 3 cut(s) 245, 336, 546
BbsI GAAGAC 1 cut(s) 847
Bbv12I GWGCWC 2 cut(s) 868, 1200
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 692
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 4 cut(s) 990, 1024, 1424, 1458
BciT130I CCWGG 1 cut(s) 1451
BciVI GTATCC 1 cut(s) 1589
BcoDI GTCTC 2 cut(s) 1100, 1396
BfaI CTAG 3 cut(s) 618, 623, 705
BfmI CTRYAG 3 cut(s) 72, 219, 1423
BfoI RGCGCY 1 cut(s) 438
BfuI GTATCC 1 cut(s) 1589
BisI GCNGC 4 cut(s) 225, 255, 559, 562
BlpI GCTNAGC 1 cut(s) 593
BlsI GCNGC 4 cut(s) 226, 256, 560, 563
BmcAI AGTACT 1 cut(s) 1499
Bme1390I CCNGG 1 cut(s) 1451
Bme18I GGWCC 1 cut(s) 670
BmgBI CACGTC 1 cut(s) 363
BmgT120I GGNCC 4 cut(s) 670, 681, 991, 1275
BmiI GGNNCC 4 cut(s) 744, 1066, 1143, 1217
BmrFI CCNGG 1 cut(s) 1451
BmrI ACTGGG 1 cut(s) 998
BmsI GCATC 1 cut(s) 604
BmtI GCTAGC 1 cut(s) 708
BmuI ACTGGG 1 cut(s) 998
BpiI GAAGAC 1 cut(s) 847
BpmI CTGGAG 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 593
BpuEI CTTGAG 1 cut(s) 719
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 3 cut(s) 62, 894, 1539
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 7 cut(s) 533, 679, 937, 988, 989, 1226, 1273
Bse1I ACTGG 3 cut(s) 20, 746, 993
BseBI CCWGG 1 cut(s) 1451
BseDI CCNNGG 3 cut(s) 62, 894, 1539
BseGI GGATG 2 cut(s) 373, 619
BseLI CCNNNNNNNGG 7 cut(s) 533, 679, 937, 988, 989, 1226, 1273
BseMII CTCAG 3 cut(s) 874, 1099, 1239
BseNI ACTGG 3 cut(s) 20, 746, 993
BseRI GAGGAG 2 cut(s) 705, 1575
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 211
BseYI CCCAGC 3 cut(s) 515, 837, 1023
BshFI GGCC 9 cut(s) 245, 257, 336, 546, 683, 930, 993, 1277, 1334
BsiHKAI GWGCWC 2 cut(s) 868, 1200
BsiSI CCGG 1 cut(s) 1278
BslFI GGGAC 2 cut(s) 253, 1041
BslI CCNNNNNNNGG 7 cut(s) 533, 679, 937, 988, 989, 1226, 1273
BsmAI GTCTC 2 cut(s) 1100, 1396
BsmFI GGGAC 2 cut(s) 253, 1041
BsnI GGCC 9 cut(s) 245, 257, 336, 546, 683, 930, 993, 1277, 1334
Bso31I GGTCTC 1 cut(s) 1100
Bsp1286I GDGCHC 3 cut(s) 375, 868, 1200
Bsp143I GATC 3 cut(s) 91, 1429, 1546
Bsp1720I GCTNAGC 1 cut(s) 593
Bsp19I CCATGG 2 cut(s) 62, 1539
BspACI CCGC 4 cut(s) 208, 254, 558, 561
BspANI GGCC 9 cut(s) 245, 257, 336, 546, 683, 930, 993, 1277, 1334
BspCNI CTCAG 3 cut(s) 873, 1098, 1240
BspLI GGNNCC 4 cut(s) 744, 1066, 1143, 1217
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 2 cut(s) 1437, 1541
BspTNI GGTCTC 1 cut(s) 1100
BsrI ACTGG 3 cut(s) 20, 746, 993
BssECI CCNNGG 3 cut(s) 62, 894, 1539
BssMI GATC 3 cut(s) 91, 1429, 1546
BssT1I CCWWGG 3 cut(s) 62, 894, 1539
Bst2UI CCWGG 1 cut(s) 1451
Bst4CI ACNGT 8 cut(s) 82, 286, 532, 766, 910, 1039, 1226, 1386
BstC8I GCNNGC 3 cut(s) 706, 995, 1091
BstDEI CTNAG 4 cut(s) 593, 860, 1085, 1248
BstDSI CCRYGG 2 cut(s) 62, 1539
BstF5I GGATG 2 cut(s) 373, 619
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 1 cut(s) 437
BstKTI GATC 3 cut(s) 94, 1432, 1549
BstMAI GTCTC 2 cut(s) 1100, 1396
BstMBI GATC 3 cut(s) 91, 1429, 1546
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 1451
BstNSI RCATGY 2 cut(s) 394, 1160
BstSCI CCNGG 1 cut(s) 1449
BstSFI CTRYAG 3 cut(s) 72, 219, 1423
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 847
BstX2I RGATCY 1 cut(s) 1429
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 1 cut(s) 1429
BsuI GTATCC 1 cut(s) 1589
BsuRI GGCC 9 cut(s) 245, 257, 336, 546, 683, 930, 993, 1277, 1334
BtgI CCRYGG 2 cut(s) 62, 1539
BtrI CACGTC 1 cut(s) 363
BtsCI GGATG 2 cut(s) 373, 619
BtsIMutI CAGTG 1 cut(s) 1035
Cac8I GCNNGC 3 cut(s) 706, 995, 1091
CfoI GCGC 1 cut(s) 437
Cfr13I GGNCC 4 cut(s) 670, 681, 991, 1275
CseI GACGC 1 cut(s) 586
Csp6I GTAC 4 cut(s) 486, 1300, 1345, 1498
CviAII CATG 9 cut(s) 63, 272, 305, 338, 391, 726, 1157, 1375, 1540
CviQI GTAC 4 cut(s) 486, 1300, 1345, 1498
DdeI CTNAG 4 cut(s) 593, 860, 1085, 1248
DpnI GATC 3 cut(s) 93, 1431, 1548
DpnII GATC 3 cut(s) 91, 1429, 1546
EaeI YGGCCR 5 cut(s) 243, 255, 334, 544, 928
Eco130I CCWWGG 3 cut(s) 62, 894, 1539
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 1 cut(s) 670
Eco47III AGCGCT 1 cut(s) 436
EcoO109I RGGNCCY 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 1449
EcoT14I CCWWGG 3 cut(s) 62, 894, 1539
ErhI CCWWGG 3 cut(s) 62, 894, 1539
FaeI CATG 9 cut(s) 66, 275, 308, 341, 394, 729, 1160, 1378, 1543
FalI AAGNNNNNCTT 2 cut(s) 1451, 1483
FaqI GGGAC 2 cut(s) 253, 1041
FatI CATG 9 cut(s) 62, 271, 304, 337, 390, 725, 1156, 1374, 1539
FauNDI CATATG 1 cut(s) 1172
Fnu4HI GCNGC 4 cut(s) 225, 255, 559, 562
FokI GGATG 2 cut(s) 380, 626
Fsp4HI GCNGC 4 cut(s) 225, 255, 559, 562
FspBI CTAG 3 cut(s) 618, 623, 705
GlaI GCGC 1 cut(s) 436
GluI GCNGC 4 cut(s) 225, 255, 559, 562
GsaI CCCAGC 3 cut(s) 519, 841, 1027
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 9 cut(s) 245, 257, 336, 546, 683, 930, 993, 1277, 1334
HapII CCGG 1 cut(s) 1278
HgaI GACGC 1 cut(s) 586
HhaI GCGC 1 cut(s) 437
Hin1II CATG 9 cut(s) 66, 275, 308, 341, 394, 729, 1160, 1378, 1543
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HinfI GANTC 7 cut(s) 146, 266, 642, 1031, 1166, 1250, 1399
HpaII CCGG 1 cut(s) 1278
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 3 cut(s) 445, 502, 814
Hpy188I TCNGA 8 cut(s) 11, 326, 367, 689, 713, 863, 1129, 1249
Hpy188III TCNNGA 4 cut(s) 55, 386, 450, 1433
Hpy8I GTNNAC 3 cut(s) 445, 502, 814
Hpy99I CGWCG 3 cut(s) 367, 602, 1312
HpyAV CCTTC 5 cut(s) 788, 1120, 1132, 1316, 1485
HpyCH4III ACNGT 8 cut(s) 82, 286, 532, 766, 910, 1039, 1226, 1386
HpyCH4IV ACGT 3 cut(s) 362, 498, 1347
HpyCH4V TGCA 6 cut(s) 74, 227, 313, 479, 833, 997
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 4 cut(s) 593, 860, 1085, 1248
HpySE526I ACGT 3 cut(s) 362, 498, 1347
Hsp92II CATG 9 cut(s) 66, 275, 308, 341, 394, 729, 1160, 1378, 1543
HspAI GCGC 1 cut(s) 435
Kzo9I GATC 3 cut(s) 91, 1429, 1546
LmnI GCTCC 2 cut(s) 452, 701
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 618, 623, 705
MaeII ACGT 3 cut(s) 362, 498, 1347
MaeIII GTNAC 4 cut(s) 625, 760, 799, 947
MalI GATC 3 cut(s) 93, 1431, 1548
MboI GATC 3 cut(s) 91, 1429, 1546
MboII GAAGA 6 cut(s) 847, 892, 1073, 1189, 1192, 1242
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 1 cut(s) 1429
MhlI GDGCHC 3 cut(s) 375, 868, 1200
MlsI TGGCCA 3 cut(s) 245, 336, 546
MluCI AATT 7 cut(s) 150, 315, 480, 1077, 1146, 1510, 1555
MluNI TGGCCA 3 cut(s) 245, 336, 546
MlyI GAGTC 2 cut(s) 275, 1259
MmeI TCCRAC 3 cut(s) 345, 551, 1188
Mox20I TGGCCA 3 cut(s) 245, 336, 546
MroXI GAANNNNTTC 4 cut(s) 82, 1080, 1146, 1499
MscI TGGCCA 3 cut(s) 245, 336, 546
MseI TTAA 6 cut(s) 201, 318, 396, 402, 417, 1355
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 3 cut(s) 245, 336, 546
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 1 cut(s) 1278
MspR9I CCNGG 1 cut(s) 1451
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 1451
MwoI GCNNNNNNNGC 1 cut(s) 251
NcoI CCATGG 2 cut(s) 62, 1539
NdeI CATATG 1 cut(s) 1172
NdeII GATC 3 cut(s) 91, 1429, 1546
NheI GCTAGC 1 cut(s) 704
NlaIII CATG 9 cut(s) 66, 275, 308, 341, 394, 729, 1160, 1378, 1543
NlaIV GGNNCC 4 cut(s) 744, 1066, 1143, 1217
NmuCI GTSAC 2 cut(s) 760, 947
NspI RCATGY 2 cut(s) 394, 1160
PciI ACATGT 2 cut(s) 390, 1156
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PdmI GAANNNNTTC 4 cut(s) 82, 1080, 1146, 1499
PfeI GAWTC 5 cut(s) 146, 642, 1031, 1166, 1399
PflMI CCANNNNNTGG 2 cut(s) 533, 937
PkrI GCNGC 4 cut(s) 226, 256, 560, 563
PleI GAGTC 2 cut(s) 274, 1258
PpsI GAGTC 2 cut(s) 274, 1258
PpuMI RGGWCCY 1 cut(s) 670
PscI ACATGT 2 cut(s) 390, 1156
PshBI ATTAAT 1 cut(s) 201
Psp1406I AACGTT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 670
Psp6I CCWGG 1 cut(s) 1449
PspFI CCCAGC 3 cut(s) 515, 837, 1023
PspGI CCWGG 1 cut(s) 1449
PspN4I GGNNCC 4 cut(s) 744, 1066, 1143, 1217
PspPI GGNCC 4 cut(s) 670, 681, 991, 1275
PspPPI RGGWCCY 1 cut(s) 670
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 1429
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 4 cut(s) 487, 1301, 1346, 1499
RsaNI GTAC 4 cut(s) 486, 1300, 1345, 1498
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 6 cut(s) 201, 318, 396, 402, 417, 1355
SatI GCNGC 4 cut(s) 225, 255, 559, 562
Sau3AI GATC 3 cut(s) 91, 1429, 1546
Sau96I GGNCC 4 cut(s) 670, 681, 991, 1275
ScaI AGTACT 1 cut(s) 1499
SchI GAGTC 2 cut(s) 275, 1259
ScrFI CCNGG 1 cut(s) 1451
SduI GDGCHC 3 cut(s) 375, 868, 1200
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 3 cut(s) 72, 219, 1423
SinI GGWCC 1 cut(s) 670
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 7 cut(s) 150, 315, 480, 1077, 1146, 1510, 1555
SsiI CCGC 4 cut(s) 208, 254, 558, 561
SspI AATATT 1 cut(s) 886
SspMI CTAG 3 cut(s) 618, 623, 705
StyD4I CCNGG 1 cut(s) 1449
StyI CCWWGG 3 cut(s) 62, 894, 1539
TaaI ACNGT 8 cut(s) 82, 286, 532, 766, 910, 1039, 1226, 1386
TaiI ACGT 3 cut(s) 365, 501, 1350
TaqI TCGA 6 cut(s) 56, 345, 387, 600, 1434, 1545
TasI AATT 7 cut(s) 150, 315, 480, 1077, 1146, 1510, 1555
TatI WGTACW 2 cut(s) 1299, 1497
TauI GCSGC 3 cut(s) 257, 561, 564
TfiI GAWTC 5 cut(s) 146, 642, 1031, 1166, 1399
Tru1I TTAA 6 cut(s) 201, 318, 396, 402, 417, 1355
Tru9I TTAA 6 cut(s) 201, 318, 396, 402, 417, 1355
TscAI CASTG 1 cut(s) 1042
TseFI GTSAC 2 cut(s) 760, 947
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 760, 947
TspDTI ATGAA 5 cut(s) 17, 22, 714, 913, 1029
TspRI CASTG 1 cut(s) 1042
Van91I CCANNNNNTGG 2 cut(s) 533, 937
VpaK11BI GGWCC 1 cut(s) 670
VspI ATTAAT 1 cut(s) 201
XapI RAATTY 1 cut(s) 1077
XceI RCATGY 2 cut(s) 394, 1160
XmnI GAANNNNTTC 4 cut(s) 82, 1080, 1146, 1499
XspI CTAG 3 cut(s) 618, 623, 705
ZrmI AGTACT 1 cut(s) 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.