Rmu_sc0005929.1_g000016

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005929.1
OG0000956
Physical Location & Seq
Forward (+)
44503 .. 46125
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005929.1_g000016.1.cds

Sequence Viewer

Length: 1623 bp
atgaagttaccaagttctgtagcagtagtactcttcgttctctcaaccctttggtcgttctcagaatcatgggcaactacttcaaatcccattcacgaaaacttcctcaaatgccttccagaacaatctcactccaactactccatctctgaagccatttacacccgccaaaatgctacttttgaatccgttctgctggcatacatcagaaacagcagatattacacccctacaactccgaaaccatgggttattgtaacagccaagcatgaatcccatgtgcaagcaactgtgatctgcgcaaagtggcatggtttgcagattagaatccgaagtggtggccatgattacgagggtctatcgtacgtgtcatatgtcccctttgttattcttgacatgtttaacctccgcgccattcatattgatgtcgcggatgagagtgcttgggttgagtctggggctattgtaggtgaactttactatgaaattgcaaagaaaagtaaagttcatggcttttcagctgcaactggtccaactgttggtgttggtggtcattttagtggtgcagggtatggtgtcatgctgagaaaatatgggctcacagtggataacattgaagatgctaaactagttaatgtgaatggtacaattcttgacagagagtctatgggagaagatcttttttgggccattagaggaggtggtggagcaagctttggagtcattttgtcatggaaaataaagctacttccagtgtcagccaaggtgactgtattcaacatcggaaggacccttgagcaaggcgctacagatgttctttaccggtggcaacatgtggcgcctaatctacctaaagaggtcttcatcagagcaatgcctcaagtgttgagtactagtttgcgtaacaatactttggaagtgtcattcattggtttcttcttgggacaaagtgatgagcttctttcactgataaatgggagtcttcctgaattgggtttgcagaaaacagattgctttgaaatgagttgggtagaatctacggtattttgggatgatcacccagttggaacttccatagatgttctactcgaaaggcccagggggccagaccacagcatgaaaggtaagtctgactatgtgaaagaaccaattccaaaacaagacatagaatacatattgaaggagctacttaagatggagagcggttggatgcaatggagcccttacggtggaagaatgagtgagatttccgagtgggcaactcccttccctcaccgagccggaaacctgttcatgattcaatattcttggtcgtgggaagaagaagggattgaggccacaaacaagtaccttagcttatctagaaattttcacaaaataatgactccatttgtgtccaagcgaccaagagaggccttccaaaactatagagatcttgacattggggccaatttggataatcagaccatctttaacattgctagagtctatggaagcaaatactttgaaggtaattttgaaaggctggtacgtgtgaagaccgaggttgatccccaaaactttttcaagcatgaacaaagtatcccacctcttcctcagtattatttggtccaatggtattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

540

Amino Acids

61.31

Weight (kDa)

6.13

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 838
AccB7I CCANNNNNTGG 1 cut(s) 539
AccBSI CCGCTC 1 cut(s) 1203
AccII CGCG 2 cut(s) 411, 431
AciI CCGC 4 cut(s) 166, 409, 431, 1203
AclWI GGATC 1 cut(s) 1544
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 1 cut(s) 1366
AcuI CTGAAG 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 6 cut(s) 30, 365, 646, 892, 1349, 1530
AfiI CCNNNNNNNGG 3 cut(s) 539, 992, 1229
AflII CTTAAG 1 cut(s) 1190
AflIII ACRYGT 4 cut(s) 366, 396, 832, 1531
AgeI ACCGGT 1 cut(s) 822
AhdI GACNNNNNGTC 1 cut(s) 661
AhlI ACTAGT 2 cut(s) 628, 893
AjnI CCWGG 1 cut(s) 1097
AloI GAACNNNNNNTCC 2 cut(s) 1566, 1598
AluBI AGCT 6 cut(s) 521, 714, 745, 958, 1186, 1356
AluI AGCT 6 cut(s) 521, 714, 745, 958, 1186, 1356
AlwI GGATC 1 cut(s) 1544
AoxI GGCC 7 cut(s) 340, 687, 1094, 1103, 1335, 1413, 1446
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 1366
AsiGI ACCGGT 1 cut(s) 822
Asp700I GAANNNNTTC 2 cut(s) 189, 1149
AspLEI GCGC 4 cut(s) 302, 413, 806, 841
AspS9I GGNCC 7 cut(s) 530, 687, 789, 1095, 1103, 1446, 1609
AsuHPI GGTGA 4 cut(s) 482, 778, 1049, 1265
AvaII GGWCC 3 cut(s) 530, 789, 1609
BalI TGGCCA 1 cut(s) 342
BanI GGYRCC 1 cut(s) 838
BanII GRGCYC 2 cut(s) 600, 1223
BbsI GAAGAC 3 cut(s) 853, 974, 1544
BbvI GCAGC 1 cut(s) 508
BccI CCATC 3 cut(s) 152, 1189, 1475
BciT130I CCWGG 1 cut(s) 1099
BciVI GTATCC 1 cut(s) 1592
BclI TGATCA 1 cut(s) 1054
BcuI ACTAGT 2 cut(s) 628, 893
BfaI CTAG 4 cut(s) 629, 894, 1362, 1482
BfmI CTRYAG 3 cut(s) 18, 807, 1426
BfoI RGCGCY 2 cut(s) 807, 842
BfrI CTTAAG 1 cut(s) 1190
BfuI GTATCC 1 cut(s) 1592
BglI GCCNNNNNGGC 1 cut(s) 1102
BglII AGATCT 2 cut(s) 676, 1432
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
BmcAI AGTACT 2 cut(s) 30, 892
Bme1390I CCNGG 1 cut(s) 1099
Bme18I GGWCC 3 cut(s) 530, 789, 1609
BmeRI GACNNNNNGTC 1 cut(s) 661
BmgT120I GGNCC 7 cut(s) 530, 687, 789, 1095, 1103, 1446, 1609
BmiI GGNNCC 5 cut(s) 791, 840, 1104, 1220, 1447
BmrFI CCNGG 1 cut(s) 1099
BmrI ACTGGG 1 cut(s) 1055
BmsI GCATC 2 cut(s) 610, 1200
BmuI ACTGGG 1 cut(s) 1055
BpiI GAAGAC 3 cut(s) 853, 974, 1544
Bpu10I CCTNAGC 1 cut(s) 1352
BpuEI CTTGAG 2 cut(s) 815, 864
BsaAI YACGTR 2 cut(s) 367, 1532
BsaBI GATNNNNATC 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 839
BsaJI CCNNGG 5 cut(s) 245, 762, 1097, 1098, 1542
BsaWI WCCGGW 1 cut(s) 822
Bsc4I CCNNNNNNNGG 3 cut(s) 539, 992, 1229
Bse118I RCCGGY 1 cut(s) 822
Bse1I ACTGG 3 cut(s) 532, 752, 1061
Bse3DI GCAATG 3 cut(s) 879, 1220, 1476
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 1 cut(s) 1099
BseDI CCNNGG 5 cut(s) 245, 762, 1097, 1098, 1542
BseGI GGATG 3 cut(s) 439, 1057, 1215
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 3 cut(s) 539, 992, 1229
BseMI GCAATG 3 cut(s) 879, 1220, 1476
BseMII CTCAG 3 cut(s) 75, 575, 1610
BseNI ACTGG 3 cut(s) 532, 752, 1061
BseRI GAGGAG 1 cut(s) 711
BseXI GCAGC 1 cut(s) 508
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 2 cut(s) 411, 431
BshFI GGCC 7 cut(s) 342, 689, 1096, 1105, 1337, 1415, 1448
BshNI GGYRCC 1 cut(s) 838
BshTI ACCGGT 1 cut(s) 822
BsiSI CCGG 2 cut(s) 823, 1281
BsiWI CGTACG 1 cut(s) 363
BslFI GGGAC 2 cut(s) 362, 957
BslI CCNNNNNNNGG 3 cut(s) 539, 992, 1229
BsmFI GGGAC 2 cut(s) 362, 957
BsnI GGCC 7 cut(s) 342, 689, 1096, 1105, 1337, 1415, 1448
Bsp1286I GDGCHC 2 cut(s) 600, 1223
Bsp143I GATC 5 cut(s) 294, 676, 1054, 1432, 1549
Bsp19I CCATGG 1 cut(s) 245
BspACI CCGC 4 cut(s) 166, 409, 431, 1203
BspANI GGCC 7 cut(s) 342, 689, 1096, 1105, 1337, 1415, 1448
BspCNI CTCAG 3 cut(s) 74, 576, 1609
BspFNI CGCG 2 cut(s) 411, 431
BspHI TCATGA 1 cut(s) 1293
BspLI GGNNCC 5 cut(s) 791, 840, 1104, 1220, 1447
BspPI GGATC 1 cut(s) 1544
BspT107I GGYRCC 1 cut(s) 838
BspTI CTTAAG 1 cut(s) 1190
BsrBI CCGCTC 1 cut(s) 1203
BsrDI GCAATG 3 cut(s) 879, 1220, 1476
BsrFI RCCGGY 1 cut(s) 822
BsrI ACTGG 3 cut(s) 532, 752, 1061
BssAI RCCGGY 1 cut(s) 822
BssECI CCNNGG 5 cut(s) 245, 762, 1097, 1098, 1542
BssMI GATC 5 cut(s) 294, 676, 1054, 1432, 1549
BssNI GRCGYC 1 cut(s) 839
BssT1I CCWWGG 2 cut(s) 245, 762
Bst2UI CCWGG 1 cut(s) 1099
Bst4CI ACNGT 6 cut(s) 292, 538, 604, 772, 1042, 1229
Bst6I CTCTTC 2 cut(s) 38, 1596
BstACI GRCGYC 1 cut(s) 839
BstAFI CTTAAG 1 cut(s) 1190
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBAI YACGTR 2 cut(s) 367, 1532
BstC8I GCNNGC 3 cut(s) 198, 285, 712
BstDEI CTNAG 4 cut(s) 61, 584, 1352, 1596
BstDSI CCRYGG 1 cut(s) 245
BstF5I GGATG 3 cut(s) 439, 1057, 1215
BstFNI CGCG 2 cut(s) 411, 431
BstH2I RGCGCY 2 cut(s) 807, 842
BstHHI GCGC 4 cut(s) 302, 413, 806, 841
BstKTI GATC 5 cut(s) 297, 679, 1057, 1435, 1552
BstMBI GATC 5 cut(s) 294, 676, 1054, 1432, 1549
BstMWI GCNNNNNNNGC 2 cut(s) 316, 1102
BstNI CCWGG 1 cut(s) 1099
BstNSI RCATGY 2 cut(s) 400, 836
BstSCI CCNGG 1 cut(s) 1097
BstSFI CTRYAG 3 cut(s) 18, 807, 1426
BstUI CGCG 2 cut(s) 411, 431
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 3 cut(s) 853, 974, 1544
BstX2I RGATCY 2 cut(s) 676, 1432
BstYI RGATCY 2 cut(s) 676, 1432
BsuI GTATCC 1 cut(s) 1592
BsuRI GGCC 7 cut(s) 342, 689, 1096, 1105, 1337, 1415, 1448
BtgI CCRYGG 1 cut(s) 245
BtsCI GGATG 3 cut(s) 439, 1057, 1215
BtsIMutI CAGTG 3 cut(s) 609, 759, 965
Cac8I GCNNGC 3 cut(s) 198, 285, 712
CciI TCATGA 1 cut(s) 1293
CfoI GCGC 4 cut(s) 302, 413, 806, 841
Cfr10I RCCGGY 1 cut(s) 822
Cfr13I GGNCC 7 cut(s) 530, 687, 789, 1095, 1103, 1446, 1609
Csp6I GTAC 6 cut(s) 29, 364, 645, 891, 1348, 1529
CspAI ACCGGT 1 cut(s) 822
CviQI GTAC 6 cut(s) 29, 364, 645, 891, 1348, 1529
DdeI CTNAG 4 cut(s) 61, 584, 1352, 1596
DinI GGCGCC 1 cut(s) 840
DpnI GATC 5 cut(s) 296, 678, 1056, 1434, 1551
DpnII GATC 5 cut(s) 294, 676, 1054, 1432, 1549
DriI GACNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 38, 1596
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 2 cut(s) 38, 1596
Eco130I CCWWGG 2 cut(s) 245, 762
Eco147I AGGCCT 1 cut(s) 1415
Eco24I GRGCYC 2 cut(s) 600, 1223
Eco47I GGWCC 3 cut(s) 530, 789, 1609
Eco57I CTGAAG 1 cut(s) 171
EcoO109I RGGNCCY 1 cut(s) 789
EcoRII CCWGG 1 cut(s) 1097
EcoT14I CCWWGG 2 cut(s) 245, 762
EcoT38I GRGCYC 2 cut(s) 600, 1223
EgeI GGCGCC 1 cut(s) 840
EheI GGCGCC 1 cut(s) 840
ErhI CCWWGG 2 cut(s) 245, 762
FaqI GGGAC 2 cut(s) 362, 957
FauI CCCGC 1 cut(s) 173
FauNDI CATATG 1 cut(s) 373
FbaI TGATCA 1 cut(s) 1054
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 3 cut(s) 446, 1064, 1222
FriOI GRGCYC 2 cut(s) 600, 1223
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 4 cut(s) 629, 894, 1362, 1482
FspI TGCGCA 1 cut(s) 301
GlaI GCGC 4 cut(s) 301, 412, 805, 840
GluI GCNGC 1 cut(s) 522
HaeII RGCGCY 2 cut(s) 807, 842
HaeIII GGCC 7 cut(s) 342, 689, 1096, 1105, 1337, 1415, 1448
HapII CCGG 2 cut(s) 823, 1281
HhaI GCGC 4 cut(s) 302, 413, 806, 841
Hin1I GRCGYC 1 cut(s) 839
Hin6I GCGC 4 cut(s) 300, 411, 804, 839
HinP1I GCGC 4 cut(s) 300, 411, 804, 839
HindIII AAGCTT 1 cut(s) 712
HpaII CCGG 2 cut(s) 823, 1281
HphI GGTGA 4 cut(s) 482, 778, 1049, 1265
Hpy166II GTNNAC 1 cut(s) 473
Hpy188III TCNNGA 8 cut(s) 95, 119, 392, 653, 986, 1294, 1362, 1436
Hpy8I GTNNAC 1 cut(s) 473
HpyAV CCTTC 7 cut(s) 125, 780, 1174, 1276, 1319, 1426, 1502
HpyCH4III ACNGT 6 cut(s) 292, 538, 604, 772, 1042, 1229
HpyCH4IV ACGT 2 cut(s) 366, 1531
HpyCH4V TGCA 7 cut(s) 283, 319, 491, 524, 566, 1000, 1213
HpyF10VI GCNNNNNNNGC 2 cut(s) 316, 1102
HpyF3I CTNAG 4 cut(s) 61, 584, 1352, 1596
HpySE526I ACGT 2 cut(s) 366, 1531
Hsp92I GRCGYC 1 cut(s) 839
HspAI GCGC 4 cut(s) 300, 411, 804, 839
KasI GGCGCC 1 cut(s) 838
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 5 cut(s) 294, 676, 1054, 1432, 1549
LmnI GCTCC 3 cut(s) 707, 1183, 1218
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 2 cut(s) 610, 1200
MaeI CTAG 4 cut(s) 629, 894, 1362, 1482
MaeII ACGT 2 cut(s) 366, 1531
MaeIII GTNAC 4 cut(s) 6, 256, 766, 902
MalI GATC 5 cut(s) 296, 678, 1056, 1434, 1551
MbiI CCGCTC 1 cut(s) 1203
MboI GATC 5 cut(s) 294, 676, 1054, 1432, 1549
MflI RGATCY 2 cut(s) 676, 1432
MhlI GDGCHC 2 cut(s) 600, 1223
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 7 cut(s) 486, 648, 989, 1149, 1366, 1450, 1513
MluNI TGGCCA 1 cut(s) 342
Mly113I GGCGCC 1 cut(s) 839
MlyI GAGTC 6 cut(s) 461, 671, 729, 988, 1378, 1494
MmeI TCCRAC 4 cut(s) 159, 557, 1045, 1187
Mox20I TGGCCA 1 cut(s) 342
MroXI GAANNNNTTC 2 cut(s) 189, 1149
MscI TGGCCA 1 cut(s) 342
MseI TTAA 5 cut(s) 402, 633, 1191, 1473, 1621
MslI CAYNNNNRTG 2 cut(s) 423, 558
Msp20I TGGCCA 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 521
MspCI CTTAAG 1 cut(s) 1190
MspI CCGG 2 cut(s) 823, 1281
MspR9I CCNGG 1 cut(s) 1099
MvaI CCWGG 1 cut(s) 1099
MvnI CGCG 2 cut(s) 411, 431
MwoI GCNNNNNNNGC 2 cut(s) 316, 1102
NarI GGCGCC 1 cut(s) 839
NcoI CCATGG 1 cut(s) 245
NdeI CATATG 1 cut(s) 373
NdeII GATC 5 cut(s) 294, 676, 1054, 1432, 1549
NlaIV GGNNCC 5 cut(s) 791, 840, 1104, 1220, 1447
NmuCI GTSAC 1 cut(s) 766
NsbI TGCGCA 1 cut(s) 301
NspI RCATGY 2 cut(s) 400, 836
PagI TCATGA 1 cut(s) 1293
PasI CCCWGGG 1 cut(s) 1098
PceI AGGCCT 1 cut(s) 1415
PciI ACATGT 2 cut(s) 396, 832
PdmI GAANNNNTTC 2 cut(s) 189, 1149
PfeI GAWTC 6 cut(s) 65, 185, 272, 327, 1034, 1297
Pfl23II CGTACG 1 cut(s) 363
PflMI CCANNNNNTGG 1 cut(s) 539
PinAI ACCGGT 1 cut(s) 822
PkrI GCNGC 1 cut(s) 523
PleI GAGTC 6 cut(s) 460, 670, 728, 987, 1378, 1493
PluTI GGCGCC 1 cut(s) 842
PpsI GAGTC 6 cut(s) 460, 670, 728, 987, 1378, 1493
Ppu21I YACGTR 2 cut(s) 367, 1532
PpuMI RGGWCCY 1 cut(s) 789
PscI ACATGT 2 cut(s) 396, 832
Psp5II RGGWCCY 1 cut(s) 789
Psp6I CCWGG 1 cut(s) 1097
PspGI CCWGG 1 cut(s) 1097
PspLI CGTACG 1 cut(s) 363
PspN4I GGNNCC 5 cut(s) 791, 840, 1104, 1220, 1447
PspPI GGNCC 7 cut(s) 530, 687, 789, 1095, 1103, 1446, 1609
PspPPI RGGWCCY 1 cut(s) 789
PsrI GAACNNNNNNTAC 2 cut(s) 21, 53
PsuI RGATCY 2 cut(s) 676, 1432
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 6 cut(s) 30, 365, 646, 892, 1349, 1530
RsaNI GTAC 6 cut(s) 29, 364, 645, 891, 1348, 1529
RseI CAYNNNNRTG 2 cut(s) 423, 558
SaqAI TTAA 5 cut(s) 402, 633, 1191, 1473, 1621
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 5 cut(s) 294, 676, 1054, 1432, 1549
Sau96I GGNCC 7 cut(s) 530, 687, 789, 1095, 1103, 1446, 1609
ScaI AGTACT 2 cut(s) 30, 892
SchI GAGTC 6 cut(s) 461, 671, 729, 988, 1378, 1494
ScrFI CCNGG 1 cut(s) 1099
SduI GDGCHC 2 cut(s) 600, 1223
SfaNI GCATC 2 cut(s) 610, 1200
SfcI CTRYAG 3 cut(s) 18, 807, 1426
SfiI GGCCNNNNNGGCC 1 cut(s) 1102
SfoI GGCGCC 1 cut(s) 840
SinI GGWCC 3 cut(s) 530, 789, 1609
SmiMI CAYNNNNRTG 2 cut(s) 423, 558
SmlI CTYRAG 3 cut(s) 794, 879, 1190
SmoI CTYRAG 3 cut(s) 794, 879, 1190
SpeI ACTAGT 2 cut(s) 628, 893
Sse9I AATT 7 cut(s) 486, 648, 989, 1149, 1366, 1450, 1513
SseBI AGGCCT 1 cut(s) 1415
SsiI CCGC 4 cut(s) 166, 409, 431, 1203
SspDI GGCGCC 1 cut(s) 838
SspI AATATT 1 cut(s) 1304
SspMI CTAG 4 cut(s) 629, 894, 1362, 1482
StuI AGGCCT 1 cut(s) 1415
StyD4I CCNGG 1 cut(s) 1097
StyI CCWWGG 2 cut(s) 245, 762
TaaI ACNGT 6 cut(s) 292, 538, 604, 772, 1042, 1229
TaiI ACGT 2 cut(s) 369, 1534
TaqI TCGA 1 cut(s) 1089
TaqII GACCGA 1 cut(s) 1556
TasI AATT 7 cut(s) 486, 648, 989, 1149, 1366, 1450, 1513
TatI WGTACW 2 cut(s) 28, 890
TfiI GAWTC 6 cut(s) 65, 185, 272, 327, 1034, 1297
Tru1I TTAA 5 cut(s) 402, 633, 1191, 1473, 1621
Tru9I TTAA 5 cut(s) 402, 633, 1191, 1473, 1621
TscAI CASTG 3 cut(s) 609, 759, 972
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 1 cut(s) 766
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 3 cut(s) 609, 759, 972
Van91I CCANNNNNTGG 1 cut(s) 539
Vha464I CTTAAG 1 cut(s) 1190
VpaK11BI GGWCC 3 cut(s) 530, 789, 1609
XapI RAATTY 1 cut(s) 1366
XbaI TCTAGA 1 cut(s) 1361
XceI RCATGY 2 cut(s) 400, 836
XmnI GAANNNNTTC 2 cut(s) 189, 1149
XspI CTAG 4 cut(s) 629, 894, 1362, 1482
ZrmI AGTACT 2 cut(s) 30, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.