RchiOBHm_Chr5g0017831

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000956
Physical Location & Seq
Reverse (-)
12417603 .. 12419195
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29806

Sequence Viewer

Length: 1593 bp
ATGAGGGTGCTACTGCTCATTATTCTATCATTCCTTTGTTTTTTAGTATCATGTACAACTACTTCAGATCCAATTCTCAACGATTTTCTCCGCTGCCTTACAAATCATTCACGGTACTCCACCTACCCAATCTCTGAAGCCATTATCACCTACCAAAGCACCTCTTTTCAATCTGCTCTCTTGGCATACATTAGGAACCGCAGATATTCCACACCCACAACACCAAAACCTTTGGTGATTGTAGCAGCCAAGCATGAATCCCATGTTCAATCGACTGTCATTTGTGCACAACAACATGGATTTCAAATAAGAACCCGAAGCGGTGGCCATGATTTTGAGGGTCTATCGTACGTGTCAAGTGCCACCCCCTTTGTCATGCTTGACATGTCTAATCTTAGATCCATCGATATTAATGTCACGGACGAGAGTGCTTGGGTCGAATCTGGGGCTACTCTTGGTGAACTTTATTTCAATGTTGGAAACAAAAGCAATATTCATGGGTTTCCTGCTGGAGTTTGCCCTACTGTTGGTATCGGTGGCCATCTTAGTGGTGGTGGTTATGGCCCTTTGATAAGAAAATATGGACTTACAGTGGATAACATTGTAGATGCTAAGCTAGTGAATGTGAAAGGTACAATTCTTAATAGAAAATCCATGGGAGAAGACCTTTTCTGGGCCATTAGAGGAGGTGGTGGAGCAAGCTTTGGAGTCGTTGTTTCTTGGAAGATCAAATTGGTTCCAGTTCCAACCAAAGTGACTGTATTCAATGTTTTAAGGACCTTGGAACACGGCGCTATAGATATCGTTTATCGGTGGCAATACGTAGCGCCTAAACTGCCTAAAGAGATCTTCATTAGGGCAATGCTTCAAGTGAAGAAAAATAGTACTTCAGGTAAGTTGGCTGTGGAAGTGTCATTCATCTGTCATTATTTGGGAGATAATGAGAAACTTCTTTCATTGATGAATCGAAATTTTCCTGAATTGGGTTTGCAGCAAAATGATTGCTTTGAAATGAGTTGGGTAGAATCTACTGTGTTCTGGGGTAACCACCCAATTGGAACTCCCATAGCTGTTCTGCTTGACAGGCCGATGGGGCCACCAGATTTCTTCAAGAGCAAGTCAGACTTTGTGAAAGAGCCGATTCCGAAACATGGAATAGAATCCATATTGGACTTGATGCTAAAGACGGGTCTAGATAAACTGTACATGGAATGGAACCCTTATGGTGGAAGAATGAGTGAGATTTCAGAGTCGGAAACTCCATTCCCTCACAGGGCTGGAAACCTGTTTTTGATTCAGTATTTGGGATATTGGAAAGAAGACAAGGCTGAGACAGCCCACAGCTACCTTGACTCGATTAGCAAAATGTATCAAGAAATGACTGCATTTGTGTCCAAGACCCCAAGAGAGGCCTTCCTAAACTATAGGGATCTTGACCTTGGGGCCAGTAAGGGTAATCAGACTAAAGTTGAAACTGCAAGAGTTTATGGAAGCATGTATTTTAAAGAAAACTTTGATAGACTGGTACGTGTGAAAACTGCGGTTGATCCTCAGAATTTTTTCAAGAATGAACAGAGCATCCCACCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

530

Amino Acids

59.41

Weight (kDa)

8.61

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 77 - 214 1.8e-25 FAD binding domain
BBE PF08031 471 - 528 3.5e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 91, 199, 321, 1541
AclWI GGATC 4 cut(s) 62, 393, 1437, 1541
AcoI YGGCCR 2 cut(s) 325, 538
AcsI RAATTY 2 cut(s) 970, 1555
AcuI CTGAAG 3 cut(s) 48, 156, 873
AfaI GTAC 7 cut(s) 55, 116, 350, 634, 886, 1205, 1527
AfiI CCNNNNNNNGG 7 cut(s) 527, 673, 983, 1151, 1226, 1273, 1408
AflIII ACRYGT 3 cut(s) 351, 384, 1528
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AloI GAACNNNNNNTCC 1 cut(s) 1563
AluBI AGCT 4 cut(s) 616, 702, 1070, 1344
AluI AGCT 4 cut(s) 616, 702, 1070, 1344
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 1325
Alw44I GTGCAC 1 cut(s) 285
AlwI GGATC 4 cut(s) 62, 393, 1437, 1541
AoxI GGCC 8 cut(s) 325, 538, 562, 675, 1085, 1094, 1410, 1443
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 3 cut(s) 93, 245, 991
ApoI RAATTY 2 cut(s) 970, 1555
AseI ATTAAT 1 cut(s) 411
Asp700I GAANNNNTTC 1 cut(s) 1559
AspLEI GCGC 2 cut(s) 794, 829
AspS9I GGNCC 5 cut(s) 563, 675, 777, 1094, 1443
AsuHPI GGTGA 3 cut(s) 139, 247, 470
AvaII GGWCC 1 cut(s) 777
BaeGI GKGCMC 1 cut(s) 289
BalI TGGCCA 2 cut(s) 327, 540
BarI GAAGNNNNNNTAC 2 cut(s) 46, 78
BbsI GAAGAC 2 cut(s) 669, 1326
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 3 cut(s) 80, 257, 1003
BccI CCATC 3 cut(s) 410, 549, 1084
BceAI ACGGC 1 cut(s) 805
BcoDI GTCTC 1 cut(s) 1325
BfaI CTAG 2 cut(s) 617, 1193
BfmI CTRYAG 3 cut(s) 795, 1423, 1589
BfoI RGCGCY 2 cut(s) 795, 830
BglI GCCNNNNNGGC 1 cut(s) 1093
BglII AGATCT 1 cut(s) 846
BisI GCNGC 3 cut(s) 94, 246, 992
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 3 cut(s) 95, 247, 993
BmcAI AGTACT 1 cut(s) 886
Bme18I GGWCC 1 cut(s) 777
BmgT120I GGNCC 5 cut(s) 563, 675, 777, 1094, 1443
BmiI GGNNCC 5 cut(s) 197, 738, 1095, 1217, 1444
BmsI GCATC 3 cut(s) 598, 1167, 1587
BpiI GAAGAC 2 cut(s) 669, 1326
BpmI CTGGAG 1 cut(s) 531
Bpu1102I GCTNAGC 1 cut(s) 612
Bsa29I ATCGAT 1 cut(s) 405
BsaAI YACGTR 3 cut(s) 352, 823, 1529
BsaJI CCNNGG 3 cut(s) 654, 780, 1438
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 7 cut(s) 527, 673, 983, 1151, 1226, 1273, 1408
Bse1I ACTGG 3 cut(s) 740, 1446, 1527
Bse3DI GCAATG 1 cut(s) 867
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 3 cut(s) 654, 780, 1438
BseGI GGATG 1 cut(s) 1578
BseLI CCNNNNNNNGG 7 cut(s) 527, 673, 983, 1151, 1226, 1273, 1408
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 2 cut(s) 1320, 1565
BseNI ACTGG 3 cut(s) 740, 1446, 1527
BseRI GAGGAG 1 cut(s) 699
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 3 cut(s) 80, 257, 1003
BshFI GGCC 8 cut(s) 327, 540, 564, 677, 1087, 1096, 1412, 1445
BshVI ATCGAT 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 289
BsiWI CGTACG 1 cut(s) 348
BslI CCNNNNNNNGG 7 cut(s) 527, 673, 983, 1151, 1226, 1273, 1408
BsmAI GTCTC 1 cut(s) 1325
BsnI GGCC 8 cut(s) 327, 540, 564, 677, 1087, 1096, 1412, 1445
Bsp1286I GDGCHC 1 cut(s) 289
Bsp1407I TGTACA 2 cut(s) 53, 1203
Bsp143I GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
Bsp1720I GCTNAGC 1 cut(s) 612
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 4 cut(s) 91, 199, 321, 1541
BspANI GGCC 8 cut(s) 327, 540, 564, 677, 1087, 1096, 1412, 1445
BspCNI CTCAG 2 cut(s) 1321, 1564
BspDI ATCGAT 1 cut(s) 405
BspLI GGNNCC 5 cut(s) 197, 738, 1095, 1217, 1444
BspPI GGATC 4 cut(s) 62, 393, 1437, 1541
BsrDI GCAATG 1 cut(s) 867
BsrGI TGTACA 2 cut(s) 53, 1203
BsrI ACTGG 3 cut(s) 740, 1446, 1527
BssECI CCNNGG 3 cut(s) 654, 780, 1438
BssMI GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
BssT1I CCWWGG 3 cut(s) 654, 780, 1438
Bst4CI ACNGT 8 cut(s) 114, 277, 526, 592, 760, 1033, 1203, 1590
BstAUI TGTACA 2 cut(s) 53, 1203
BstBAI YACGTR 3 cut(s) 352, 823, 1529
BstC8I GCNNGC 1 cut(s) 700
BstDEI CTNAG 5 cut(s) 395, 545, 612, 1329, 1551
BstDSI CCRYGG 1 cut(s) 654
BstEII GGTNACC 1 cut(s) 1043
BstF5I GGATG 1 cut(s) 1578
BstH2I RGCGCY 2 cut(s) 795, 830
BstHHI GCGC 2 cut(s) 794, 829
BstKTI GATC 6 cut(s) 70, 401, 729, 849, 1432, 1549
BstMAI GTCTC 1 cut(s) 1325
BstMBI GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
BstMWI GCNNNNNNNGC 5 cut(s) 182, 835, 1084, 1093, 1334
BstNSI RCATGY 2 cut(s) 388, 1498
BstPI GGTNACC 1 cut(s) 1043
BstSFI CTRYAG 3 cut(s) 795, 1423, 1589
BstSLI GKGCMC 1 cut(s) 289
BstSNI TACGTA 1 cut(s) 823
BstV1I GCAGC 3 cut(s) 80, 257, 1003
BstV2I GAAGAC 2 cut(s) 669, 1326
BstX2I RGATCY 4 cut(s) 67, 398, 846, 1429
BstXI CCANNNNNNTGG 2 cut(s) 548, 1055
BstYI RGATCY 4 cut(s) 67, 398, 846, 1429
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 8 cut(s) 327, 540, 564, 677, 1087, 1096, 1412, 1445
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 1 cut(s) 1578
BtsIMutI CAGTG 2 cut(s) 597, 1586
Cac8I GCNNGC 1 cut(s) 700
CfoI GCGC 2 cut(s) 794, 829
Cfr13I GGNCC 5 cut(s) 563, 675, 777, 1094, 1443
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 7 cut(s) 54, 115, 349, 633, 885, 1204, 1526
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviQI GTAC 7 cut(s) 54, 115, 349, 633, 885, 1204, 1526
DdeI CTNAG 5 cut(s) 395, 545, 612, 1329, 1551
DpnI GATC 6 cut(s) 69, 400, 728, 848, 1431, 1548
DpnII GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
DraI TTTAAA 1 cut(s) 1504
EaeI YGGCCR 2 cut(s) 325, 538
Eco105I TACGTA 1 cut(s) 823
Eco130I CCWWGG 3 cut(s) 654, 780, 1438
Eco147I AGGCCT 1 cut(s) 1412
Eco32I GATATC 1 cut(s) 802
Eco47I GGWCC 1 cut(s) 777
Eco57I CTGAAG 3 cut(s) 48, 156, 873
Eco91I GGTNACC 1 cut(s) 1043
EcoO109I RGGNCCY 1 cut(s) 777
EcoO65I GGTNACC 1 cut(s) 1043
EcoRV GATATC 1 cut(s) 802
EcoT14I CCWWGG 3 cut(s) 654, 780, 1438
ErhI CCWWGG 3 cut(s) 654, 780, 1438
FalI AAGNNNNNCTT 4 cut(s) 148, 180, 1109, 1141
Fnu4HI GCNGC 3 cut(s) 94, 246, 992
FokI GGATG 1 cut(s) 1565
Fsp4HI GCNGC 3 cut(s) 94, 246, 992
FspBI CTAG 2 cut(s) 617, 1193
GlaI GCGC 2 cut(s) 793, 828
GluI GCNGC 3 cut(s) 94, 246, 992
GsuI CTGGAG 1 cut(s) 531
HaeII RGCGCY 2 cut(s) 795, 830
HaeIII GGCC 8 cut(s) 327, 540, 564, 677, 1087, 1096, 1412, 1445
HhaI GCGC 2 cut(s) 794, 829
Hin6I GCGC 2 cut(s) 792, 827
HinP1I GCGC 2 cut(s) 792, 827
HindIII AAGCTT 1 cut(s) 700
HphI GGTGA 3 cut(s) 139, 247, 470
Hpy166II GTNNAC 2 cut(s) 287, 461
Hpy188I TCNGA 8 cut(s) 67, 136, 1123, 1146, 1249, 1255, 1461, 1554
Hpy188III TCNNGA 6 cut(s) 977, 1111, 1193, 1373, 1433, 1564
Hpy8I GTNNAC 2 cut(s) 287, 461
HpyAV CCTTC 1 cut(s) 1423
HpyCH4III ACNGT 8 cut(s) 114, 277, 526, 592, 760, 1033, 1203, 1590
HpyCH4IV ACGT 3 cut(s) 351, 822, 1528
HpyCH4V TGCA 4 cut(s) 287, 991, 1385, 1478
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 835, 1084, 1093, 1334
HpyF3I CTNAG 5 cut(s) 395, 545, 612, 1329, 1551
HpySE526I ACGT 3 cut(s) 351, 822, 1528
HspAI GCGC 2 cut(s) 792, 827
Kzo9I GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
LmnI GCTCC 1 cut(s) 695
Lsp1109I GCAGC 3 cut(s) 80, 257, 1003
LweI GCATC 3 cut(s) 598, 1167, 1587
MaeI CTAG 2 cut(s) 617, 1193
MaeII ACGT 3 cut(s) 351, 822, 1528
MaeIII GTNAC 3 cut(s) 415, 754, 1043
MalI GATC 6 cut(s) 69, 400, 728, 848, 1431, 1548
MboI GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
MboII GAAGA 7 cut(s) 674, 736, 841, 886, 1099, 1242, 1331
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 4 cut(s) 67, 398, 846, 1429
MhlI GDGCHC 1 cut(s) 289
MlsI TGGCCA 2 cut(s) 327, 540
MluCI AATT 7 cut(s) 72, 636, 731, 970, 980, 1053, 1555
MluNI TGGCCA 2 cut(s) 327, 540
MlyI GAGTC 3 cut(s) 717, 1259, 1346
MmeI TCCRAC 3 cut(s) 457, 770, 1233
MnlI CCTC 7 cut(s) 172, 331, 677, 680, 1278, 1402, 1560
Mox20I TGGCCA 2 cut(s) 327, 540
MroXI GAANNNNTTC 1 cut(s) 1559
MscI TGGCCA 2 cut(s) 327, 540
MseI TTAA 4 cut(s) 411, 642, 773, 1503
MslI CAYNNNNRTG 1 cut(s) 546
Msp20I TGGCCA 2 cut(s) 327, 540
MspA1I CMGCKG 1 cut(s) 93
MunI CAATTG 1 cut(s) 1053
MwoI GCNNNNNNNGC 5 cut(s) 182, 835, 1084, 1093, 1334
NcoI CCATGG 1 cut(s) 654
NdeII GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
NlaIV GGNNCC 5 cut(s) 197, 738, 1095, 1217, 1444
NmuCI GTSAC 2 cut(s) 415, 754
NspI RCATGY 2 cut(s) 388, 1498
PceI AGGCCT 1 cut(s) 1412
PciI ACATGT 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 1559
PfeI GAWTC 7 cut(s) 257, 440, 964, 1025, 1141, 1160, 1294
Pfl23II CGTACG 1 cut(s) 348
PkrI GCNGC 3 cut(s) 95, 247, 993
PleI GAGTC 3 cut(s) 716, 1258, 1346
PpsI GAGTC 3 cut(s) 716, 1258, 1346
Ppu21I YACGTR 3 cut(s) 352, 823, 1529
PpuMI RGGWCCY 1 cut(s) 777
PscI ACATGT 1 cut(s) 384
PshBI ATTAAT 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 777
PspEI GGTNACC 1 cut(s) 1043
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 5 cut(s) 197, 738, 1095, 1217, 1444
PspPI GGNCC 5 cut(s) 563, 675, 777, 1094, 1443
PspPPI RGGWCCY 1 cut(s) 777
PsuI RGATCY 4 cut(s) 67, 398, 846, 1429
RsaI GTAC 7 cut(s) 55, 116, 350, 634, 886, 1205, 1527
RsaNI GTAC 7 cut(s) 54, 115, 349, 633, 885, 1204, 1526
RseI CAYNNNNRTG 1 cut(s) 546
SaqAI TTAA 4 cut(s) 411, 642, 773, 1503
SatI GCNGC 3 cut(s) 94, 246, 992
Sau3AI GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
Sau96I GGNCC 5 cut(s) 563, 675, 777, 1094, 1443
ScaI AGTACT 1 cut(s) 886
SchI GAGTC 3 cut(s) 717, 1259, 1346
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 3 cut(s) 598, 1167, 1587
SfcI CTRYAG 3 cut(s) 795, 1423, 1589
SfiI GGCCNNNNNGGCC 1 cut(s) 1093
SinI GGWCC 1 cut(s) 777
SmiMI CAYNNNNRTG 1 cut(s) 546
SnaBI TACGTA 1 cut(s) 823
Sse9I AATT 7 cut(s) 72, 636, 731, 970, 980, 1053, 1555
SseBI AGGCCT 1 cut(s) 1412
SsiI CCGC 4 cut(s) 91, 199, 321, 1541
SspI AATATT 1 cut(s) 493
SspMI CTAG 2 cut(s) 617, 1193
StuI AGGCCT 1 cut(s) 1412
StyI CCWWGG 3 cut(s) 654, 780, 1438
TaaI ACNGT 8 cut(s) 114, 277, 526, 592, 760, 1033, 1203, 1590
TaiI ACGT 3 cut(s) 354, 825, 1531
TaqI TCGA 5 cut(s) 272, 405, 438, 967, 1355
TasI AATT 7 cut(s) 72, 636, 731, 970, 980, 1053, 1555
TatI WGTACW 3 cut(s) 53, 884, 1203
TfiI GAWTC 7 cut(s) 257, 440, 964, 1025, 1141, 1160, 1294
Tru1I TTAA 4 cut(s) 411, 642, 773, 1503
Tru9I TTAA 4 cut(s) 411, 642, 773, 1503
TscAI CASTG 2 cut(s) 597, 1593
TseFI GTSAC 2 cut(s) 415, 754
TseI GCWGC 3 cut(s) 93, 245, 991
Tsp45I GTSAC 2 cut(s) 415, 754
TspDTI ATGAA 7 cut(s) 270, 485, 841, 907, 945, 977, 1584
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 2 cut(s) 597, 1593
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 777
VspI ATTAAT 1 cut(s) 411
XapI RAATTY 2 cut(s) 970, 1555
XbaI TCTAGA 1 cut(s) 1192
XceI RCATGY 2 cut(s) 388, 1498
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 1559
XspI CTAG 2 cut(s) 617, 1193
ZrmI AGTACT 1 cut(s) 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.