RLG00000032315

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000956
Physical Location & Seq
Reverse (-)
13460787 .. 13462340
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032315

Sequence Viewer

Length: 1293 bp
ATGAGAATCCGAAGTGGTGGCCACGATTACGAGGGCTTATCCTTTATATCATATATACCAAATGTTTCCTATATAGTCCTTGACATGTTTAACCTTCGATCGATTAATATTAACGTTGAAGATGAAAGTGCTTGGGTTGAGTCTGGAGCTATCTTAGGTGAGCTTTACTATAACATTGCGGAGAAAAGCAAAGTTCATGCATTTCCTGGGGGAGTTTGTCCAACTGTTGGTGTTGGTGGCCTTTTTAGTGGTGGTGGCTATGGTCCCATGATGAGAAAATTTGGACTTACAGTGGATAATATAGAAGATGCTAGATTAGTAAACGTGAATGGTACAATCCTTGATAGAAGATCCATGGGAGAAGACCTGTTTTGGGCCATTAGAGGAGGTGGTGGAGCAAGCTTTGGAGTCATTCTTTCATGGAAGATCAAATTACTTCCAATTCCACCTAACGTGACTGTTTTCAGAATTACAAGGACACTGGAACAAGGCGCGATAGATGTTCTTTACAGATGGCAATACGTTGCGCCGAAGCTACCTAAAGACATCTTCATTAGAGCAACTTCACAACTCCATAATAGTACTAGCCAAGTGAGCAAGAAGACTGTGGAAGTGGTATTCAATGGTCTCTATGTGGGACAAAAGGAAAGCCTTCTTGCTTTGGTAAACCACAGTTTTCCTGAACTAGGTTTGGAGCAAAAATATTGCATTGAAATAAGTTGGATAAATGCCACTTTATTCTGGGCCGGCTACCCAGCTGGAACTCCCACAACTGTTCTTCTCGACAGGCCAAAGGCACCAGAAATCTTGTTCAAAAGCAGGTCTGACTATGTGAAAGAACCAATTCCGAAAGCAGCACTCGAAACCATATGGCAGAAGATACTTTCTATGGAGATGCTTCAGATGCAGTGGAACCCTTATGGTGGAAGAATGAGTGAGATTTCCGAGTCAGAGACTCCATTCCCACACAGAGCTGGGAACCTGTTTAAGATTCAGTATTACACTCTTTGGGCGAATGAAACCGACACGAAAAAGTACCTTGACTTGTCAAGCAAGTTTCGTAAAGCAATGACTCCATTTGTGTCCAAGAATCCAAGAGAGGCCTTCCAGAACTACAGAGATCTCGACAATGGTGCCAATTGGAATAACCAGACTGACACCAAGACTGCTACGGTTTATGGAAGAAAATATTTCAAAAACAATTTTTATAGATTGGTAAGTGTGAAAACCAAGGTTGATCCTGAAAACTTTTTCAGGCATGAGCAGAGTATTCCACCACTTCCCCATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

431

Amino Acids

48.8

Weight (kDa)

9.14

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 2 - 114 2.2e-16 FAD binding domain
BBE PF08031 368 - 425 3.2e-17 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 810
AccB1I GGYRCC 2 cut(s) 796, 1133
AccB7I CCANNNNNTGG 1 cut(s) 227
AccII CGCG 1 cut(s) 494
AciI CCGC 1 cut(s) 179
AclI AACGTT 1 cut(s) 114
AclWI GGATC 2 cut(s) 345, 1232
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 884
AfaI GTAC 3 cut(s) 334, 583, 1037
AfiI CCNNNNNNNGG 4 cut(s) 227, 373, 686, 923
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 5 cut(s) 119, 622, 713, 814, 1195
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 6 cut(s) 149, 163, 402, 535, 758, 974
AluI AGCT 6 cut(s) 149, 163, 402, 535, 758, 974
Alw26I GTCTC 2 cut(s) 632, 947
AlwI GGATC 2 cut(s) 345, 1232
AoxI GGCC 6 cut(s) 19, 238, 375, 744, 788, 1101
ApeKI GCWGC 1 cut(s) 854
ApoI RAATTY 1 cut(s) 278
AseI ATTAAT 1 cut(s) 105
Asp700I GAANNNNTTC 2 cut(s) 651, 843
AspLEI GCGC 2 cut(s) 494, 529
AspS9I GGNCC 3 cut(s) 263, 375, 744
AsuHPI GGTGA 1 cut(s) 170
AvaII GGWCC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 21
BanI GGYRCC 2 cut(s) 796, 1133
BbsI GAAGAC 2 cut(s) 369, 608
BbvI GCAGC 1 cut(s) 866
BccI CCATC 1 cut(s) 507
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 632, 947
BfaI CTAG 3 cut(s) 312, 585, 686
BfmI CTRYAG 1 cut(s) 1114
BfuAI ACCTGC 1 cut(s) 810
BglII AGATCT 1 cut(s) 1120
BisI GCNGC 1 cut(s) 855
BlsI GCNGC 1 cut(s) 856
BmcAI AGTACT 1 cut(s) 583
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 3 cut(s) 263, 375, 744
BmiI GGNNCC 5 cut(s) 265, 798, 914, 980, 1135
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 3 cut(s) 298, 885, 894
BpiI GAAGAC 2 cut(s) 369, 608
BpmI CTGGAG 1 cut(s) 165
Bsa29I ATCGAT 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 3 cut(s) 206, 354, 1230
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 373, 686, 923
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 1 cut(s) 486
Bse3DI GCAATG 2 cut(s) 174, 1074
BseBI CCWGG 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 101
BseDI CCNNGG 3 cut(s) 206, 354, 1230
BseLI CCNNNNNNNGG 4 cut(s) 227, 373, 686, 923
BseMI GCAATG 2 cut(s) 174, 1074
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 866
BseYI CCCAGC 2 cut(s) 754, 974
Bsh1236I CGCG 1 cut(s) 494
Bsh1285I CGRYCG 1 cut(s) 101
BshFI GGCC 6 cut(s) 21, 240, 377, 746, 790, 1103
BshNI GGYRCC 2 cut(s) 796, 1133
BshVI ATCGAT 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 101
BsiSI CCGG 1 cut(s) 747
BslFI GGGAC 2 cut(s) 249, 651
BslI CCNNNNNNNGG 4 cut(s) 227, 373, 686, 923
BsmAI GTCTC 2 cut(s) 632, 947
BsmFI GGGAC 2 cut(s) 249, 651
BsnI GGCC 6 cut(s) 21, 240, 377, 746, 790, 1103
Bso31I GGTCTC 1 cut(s) 632
Bsp143I GATC 5 cut(s) 98, 350, 426, 1120, 1237
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 1 cut(s) 179
BspANI GGCC 6 cut(s) 21, 240, 377, 746, 790, 1103
BspDI ATCGAT 1 cut(s) 101
BspFNI CGCG 1 cut(s) 494
BspLI GGNNCC 5 cut(s) 265, 798, 914, 980, 1135
BspMI ACCTGC 1 cut(s) 810
BspPI GGATC 2 cut(s) 345, 1232
BspT107I GGYRCC 2 cut(s) 796, 1133
BspTNI GGTCTC 1 cut(s) 632
BsrDI GCAATG 2 cut(s) 174, 1074
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 206, 354, 1230
BssMI GATC 5 cut(s) 98, 350, 426, 1120, 1237
BssT1I CCWWGG 2 cut(s) 354, 1230
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 7 cut(s) 226, 292, 460, 607, 674, 775, 1174
BstC8I GCNNGC 2 cut(s) 400, 748
BstDEI CTNAG 1 cut(s) 154
BstDSI CCRYGG 1 cut(s) 354
BstENI CCTNNNNNAGG 1 cut(s) 684
BstFNI CGCG 1 cut(s) 494
BstHHI GCGC 2 cut(s) 494, 529
BstKTI GATC 5 cut(s) 101, 353, 429, 1123, 1240
BstMAI GTCTC 2 cut(s) 632, 947
BstMBI GATC 5 cut(s) 98, 350, 426, 1120, 1237
BstMCI CGRYCG 1 cut(s) 101
BstMWI GCNNNNNNNGC 2 cut(s) 594, 904
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 1114
BstUI CGCG 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 866
BstV2I GAAGAC 2 cut(s) 369, 608
BstX2I RGATCY 2 cut(s) 350, 1120
BstYI RGATCY 2 cut(s) 350, 1120
Bsu15I ATCGAT 1 cut(s) 101
BsuRI GGCC 6 cut(s) 21, 240, 377, 746, 790, 1103
BsuTUI ATCGAT 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 914
BtsIMutI CAGTG 3 cut(s) 297, 479, 914
BveI ACCTGC 1 cut(s) 810
Cac8I GCNNGC 2 cut(s) 400, 748
CfoI GCGC 2 cut(s) 494, 529
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 3 cut(s) 263, 375, 744
ClaI ATCGAT 1 cut(s) 101
Csp6I GTAC 3 cut(s) 333, 582, 1036
CviAII CATG 6 cut(s) 85, 197, 268, 355, 420, 1259
CviQI GTAC 3 cut(s) 333, 582, 1036
DdeI CTNAG 1 cut(s) 154
DpnI GATC 5 cut(s) 100, 352, 428, 1122, 1239
DpnII GATC 5 cut(s) 98, 350, 426, 1120, 1237
EaeI YGGCCR 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 354, 1230
Eco147I AGGCCT 1 cut(s) 1103
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 884
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 354, 1230
EcoT22I ATGCAT 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 354, 1230
FaeI CATG 6 cut(s) 88, 200, 271, 358, 423, 1262
FaqI GGGAC 2 cut(s) 249, 651
FatI CATG 6 cut(s) 84, 196, 267, 354, 419, 1258
FauNDI CATATG 1 cut(s) 869
Fnu4HI GCNGC 1 cut(s) 855
Fsp4HI GCNGC 1 cut(s) 855
FspBI CTAG 3 cut(s) 312, 585, 686
GlaI GCGC 2 cut(s) 493, 528
GluI GCNGC 1 cut(s) 855
GsaI CCCAGC 2 cut(s) 758, 978
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 6 cut(s) 21, 240, 377, 746, 790, 1103
HapII CCGG 1 cut(s) 747
HhaI GCGC 2 cut(s) 494, 529
Hin1II CATG 6 cut(s) 88, 200, 271, 358, 423, 1262
Hin6I GCGC 2 cut(s) 492, 527
HinP1I GCGC 2 cut(s) 492, 527
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 8 cut(s) 6, 140, 408, 947, 955, 991, 1072, 1090
HpaII CCGG 1 cut(s) 747
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 2 cut(s) 322, 667
Hpy188I TCNGA 7 cut(s) 11, 467, 826, 849, 903, 946, 952
Hpy188III TCNNGA 6 cut(s) 144, 680, 782, 1108, 1124, 1241
Hpy8I GTNNAC 2 cut(s) 322, 667
HpyAV CCTTC 3 cut(s) 104, 662, 1114
HpyCH4III ACNGT 7 cut(s) 226, 292, 460, 607, 674, 775, 1174
HpyCH4IV ACGT 4 cut(s) 114, 324, 453, 522
HpyCH4V TGCA 3 cut(s) 200, 708, 907
HpyF10VI GCNNNNNNNGC 2 cut(s) 594, 904
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 4 cut(s) 114, 324, 453, 522
Hsp92II CATG 6 cut(s) 88, 200, 271, 358, 423, 1262
HspAI GCGC 2 cut(s) 492, 527
KroI GCCGGC 1 cut(s) 746
KroNI GCCGGC 1 cut(s) 748
Kzo9I GATC 5 cut(s) 98, 350, 426, 1120, 1237
LmnI GCTCC 3 cut(s) 146, 395, 694
Lsp1109I GCAGC 1 cut(s) 866
LweI GCATC 3 cut(s) 298, 885, 894
MaeI CTAG 3 cut(s) 312, 585, 686
MaeII ACGT 4 cut(s) 114, 324, 453, 522
MaeIII GTNAC 1 cut(s) 454
MalI GATC 5 cut(s) 100, 352, 428, 1122, 1239
MboI GATC 5 cut(s) 98, 350, 426, 1120, 1237
MfeI CAATTG 1 cut(s) 1138
MflI RGATCY 2 cut(s) 350, 1120
MlsI TGGCCA 1 cut(s) 21
MluCI AATT 7 cut(s) 278, 431, 441, 468, 843, 1138, 1201
MluNI TGGCCA 1 cut(s) 21
MlyI GAGTC 5 cut(s) 149, 417, 949, 956, 1066
MmeI TCCRAC 2 cut(s) 245, 701
MnlI CCTC 4 cut(s) 25, 377, 380, 1093
Mox20I TGGCCA 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 202
MroNI GCCGGC 1 cut(s) 746
MroXI GAANNNNTTC 2 cut(s) 651, 843
MscI TGGCCA 1 cut(s) 21
MseI TTAA 4 cut(s) 90, 105, 111, 987
Msp20I TGGCCA 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 1138
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 2 cut(s) 594, 904
NaeI GCCGGC 1 cut(s) 748
NcoI CCATGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 869
NdeII GATC 5 cut(s) 98, 350, 426, 1120, 1237
NgoMIV GCCGGC 1 cut(s) 746
NlaIII CATG 6 cut(s) 88, 200, 271, 358, 423, 1262
NlaIV GGNNCC 5 cut(s) 265, 798, 914, 980, 1135
NmuCI GTSAC 1 cut(s) 454
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 1 cut(s) 88
PceI AGGCCT 1 cut(s) 1103
PciI ACATGT 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 748
PdmI GAANNNNTTC 2 cut(s) 651, 843
PfeI GAWTC 3 cut(s) 6, 991, 1090
PflMI CCANNNNNTGG 1 cut(s) 227
PkrI GCNGC 1 cut(s) 856
Ple19I CGATCG 1 cut(s) 101
PleI GAGTC 5 cut(s) 148, 416, 949, 955, 1066
PpsI GAGTC 5 cut(s) 148, 416, 949, 955, 1066
PscI ACATGT 1 cut(s) 84
PshBI ATTAAT 1 cut(s) 105
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 2 cut(s) 754, 974
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 5 cut(s) 265, 798, 914, 980, 1135
PspPI GGNCC 3 cut(s) 263, 375, 744
PsuI RGATCY 2 cut(s) 350, 1120
PvuI CGATCG 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 3 cut(s) 334, 583, 1037
RsaNI GTAC 3 cut(s) 333, 582, 1036
SaqAI TTAA 4 cut(s) 90, 105, 111, 987
SatI GCNGC 1 cut(s) 855
Sau3AI GATC 5 cut(s) 98, 350, 426, 1120, 1237
Sau96I GGNCC 3 cut(s) 263, 375, 744
ScaI AGTACT 1 cut(s) 583
SchI GAGTC 5 cut(s) 149, 417, 949, 956, 1066
ScrFI CCNGG 1 cut(s) 207
SfaNI GCATC 3 cut(s) 298, 885, 894
SfcI CTRYAG 1 cut(s) 1114
SinI GGWCC 1 cut(s) 263
Sse9I AATT 7 cut(s) 278, 431, 441, 468, 843, 1138, 1201
SseBI AGGCCT 1 cut(s) 1103
SsiI CCGC 1 cut(s) 179
SspI AATATT 3 cut(s) 109, 704, 1190
SspMI CTAG 3 cut(s) 312, 585, 686
StuI AGGCCT 1 cut(s) 1103
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 2 cut(s) 354, 1230
TaaI ACNGT 7 cut(s) 226, 292, 460, 607, 674, 775, 1174
TaiI ACGT 4 cut(s) 117, 327, 456, 525
TaqI TCGA 5 cut(s) 97, 101, 783, 861, 1125
TasI AATT 7 cut(s) 278, 431, 441, 468, 843, 1138, 1201
TatI WGTACW 1 cut(s) 581
TfiI GAWTC 3 cut(s) 6, 991, 1090
Tru1I TTAA 4 cut(s) 90, 105, 111, 987
Tru9I TTAA 4 cut(s) 90, 105, 111, 987
TscAI CASTG 3 cut(s) 297, 486, 914
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 854
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 5 cut(s) 138, 185, 408, 541, 1032
TspRI CASTG 3 cut(s) 297, 486, 914
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 263
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 1 cut(s) 278
XceI RCATGY 1 cut(s) 88
XmnI GAANNNNTTC 2 cut(s) 651, 843
XspI CTAG 3 cut(s) 312, 585, 686
ZrmI AGTACT 1 cut(s) 583
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.