Rw5G011460

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000956
Physical Location & Seq
Reverse (-)
13114500 .. 13116089
1590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011460.1

Sequence Viewer

Length: 1590 bp
ATGAGGGTGCTACCGCTCATTATTCTATCATTCCTTTGTTTTTTAGTATCATGTACAACTTCAGATCCAATTCTCAACGATTTTCTCCGCTGCCTTACAAATCATTCACGGTACTCCACCTACCCAATCTCTGAAGCCATTATCACCTACCAAAACACCTCTTTTAAATCTGCTCTCTTGGCATACATTAGGAACCGCAGATATTCCACACCCACAACACCAAAACCTTTGGTGATTGTAGCAGCCAAGCATGAATCCCATGTTCAATCGACTGTCATTTGTGCACAACAACATGGATTTCAAATAAGAACCCGAAGCGGTGGCCATGATTTTGAGGGTCTATCGTACGTGTCAAGTGCCACCCCCTTTGTCATGCTTGACATGTCTAATCTTAGATCCATCGATATTAATGTCACGGACGAGAGTGCTTGGGTCGAATCTGGGGCTACTCTTGGTGAACTTTATTTCAATATTGGAAACAAAAGCAATATTCATGGGTTTCCTGCTGGAGTTTGCCCTACTGTTGGTATTGGTGGCCATCTTAGTGGTGGTGGTTATGGCCCTTTGATGAGAAAATATGGACTTACAGTGGACAACATTGTAGATGCTAAGCTAGTGAATGTGAACGGTACAATTCTTGATAGAAAATCCATGAGAGAAGACCTTTTCTGGGCCATTAGAGGAGGTGGTGGTGCAAGCTTTGGAGTCGTTGTTTCTTGGAAGATCAAATTGGTTCCAGTTCCAACCAAAGTGACTGTATTCAATGTTTTAAGGACCTTGGAACACGGCGCTATAGATATCGTTTATCGGTGGCAATACGTAGCGCCTAAACTGCCTAAAGAGATCTTCATTAGGGCAATGCTTCAAGTGAAGAAAAATAGTACTTCAGGTAAGTTGGCTGTGGAAGTGTCATTCATCTGTCATTATTTGGGAGATAATGAGAAACTTCTTTCATTGATGAATCGGAATTTTCCTGAATTGGGTTTGCAGCAAAATGATTGCTTTGAAATGAGTTGGGTAGAATCCACTGTGTTCTGGGGTAACCACCCAATTGGAACTCCTTTAGCTGTTCTTCTTGATAGGCCGATGGGGCCACCAGATTTCTTCAAGAGCAAGTCAGACTATGTGAAAGAGCCGATTCCGAAACATGGAATAGAATCCATATTGGACTTGATGCTAAAGACGGGTCTAGATAAACTGTACATGGAATGGAACCCTTATGGTGGAAGAATGAGTGAGATTTCAGAGTCGGAAACTCCATTCCCTCACAGGGCTGGAAACCTGTTTTTGATTCAGTATTTGGGATATTGGAAAGAAGACAAGGCTGAGACAGCCCACAGCTACCTTGATTTGATTAGCAAAATGTATCAAGAAATGACTCCATTTGTGTCCAAGACCCCAAGAGAGGCCTTCCTAAACTATAGGGATCTTGACCTTGGGGCCAGTAAGGGTAATCAGACTAAAGTTGAAACTGCAAGAGTTTATGGAAGCATGTATTTTAAAGAAAACTTTGATAGACTGGTACGTGTGAAAACTGCGGTTGATCCTCAGAATTTTTTCAAGAATGAACAGAGCATTCCACCACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

529

Amino Acids

59.51

Weight (kDa)

8.6

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 76 - 213 4.7e-26 FAD binding domain
BBE PF08031 470 - 527 3.5e-19 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 16
AciI CCGC 5 cut(s) 14, 88, 196, 318, 1538
AclWI GGATC 4 cut(s) 59, 390, 1434, 1538
AcoI YGGCCR 2 cut(s) 322, 535
AcsI RAATTY 2 cut(s) 967, 1552
AcuI CTGAAG 3 cut(s) 45, 153, 870
AfaI GTAC 7 cut(s) 55, 113, 347, 631, 883, 1202, 1524
AfiI CCNNNNNNNGG 7 cut(s) 524, 670, 980, 1148, 1223, 1270, 1405
AflIII ACRYGT 3 cut(s) 348, 381, 1525
AgsI TTSAA 9 cut(s) 266, 302, 469, 763, 866, 1007, 1108, 1469, 1561
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AluBI AGCT 4 cut(s) 613, 699, 1067, 1341
AluI AGCT 4 cut(s) 613, 699, 1067, 1341
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 1 cut(s) 1322
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 4 cut(s) 59, 390, 1434, 1538
AoxI GGCC 8 cut(s) 322, 535, 559, 672, 1082, 1091, 1407, 1440
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 3 cut(s) 90, 242, 988
ApoI RAATTY 2 cut(s) 967, 1552
AseI ATTAAT 1 cut(s) 408
Asp700I GAANNNNTTC 1 cut(s) 1556
AspLEI GCGC 2 cut(s) 791, 826
AspS9I GGNCC 5 cut(s) 560, 672, 774, 1091, 1440
AsuHPI GGTGA 3 cut(s) 136, 244, 467
AvaII GGWCC 1 cut(s) 774
BaeGI GKGCMC 1 cut(s) 286
BalI TGGCCA 2 cut(s) 324, 537
BbsI GAAGAC 2 cut(s) 666, 1323
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 3 cut(s) 77, 254, 1000
BccI CCATC 3 cut(s) 407, 546, 1081
BceAI ACGGC 1 cut(s) 802
BcoDI GTCTC 1 cut(s) 1322
BfaI CTAG 2 cut(s) 614, 1190
BfmI CTRYAG 3 cut(s) 792, 1420, 1586
BfoI RGCGCY 2 cut(s) 792, 827
BglI GCCNNNNNGGC 1 cut(s) 1090
BglII AGATCT 1 cut(s) 843
BisI GCNGC 3 cut(s) 91, 243, 989
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 3 cut(s) 92, 244, 990
BmcAI AGTACT 1 cut(s) 883
Bme18I GGWCC 1 cut(s) 774
BmgT120I GGNCC 5 cut(s) 560, 672, 774, 1091, 1440
BmiI GGNNCC 5 cut(s) 194, 735, 1092, 1214, 1441
BmsI GCATC 2 cut(s) 595, 1164
BpiI GAAGAC 2 cut(s) 666, 1323
BpmI CTGGAG 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 609
Bsa29I ATCGAT 1 cut(s) 402
BsaAI YACGTR 3 cut(s) 349, 820, 1526
BsaJI CCNNGG 2 cut(s) 777, 1435
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 7 cut(s) 524, 670, 980, 1148, 1223, 1270, 1405
Bse1I ACTGG 3 cut(s) 737, 1443, 1524
Bse3DI GCAATG 1 cut(s) 864
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 777, 1435
BseLI CCNNNNNNNGG 7 cut(s) 524, 670, 980, 1148, 1223, 1270, 1405
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 2 cut(s) 1317, 1562
BseNI ACTGG 3 cut(s) 737, 1443, 1524
BseRI GAGGAG 1 cut(s) 696
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 3 cut(s) 77, 254, 1000
BshFI GGCC 8 cut(s) 324, 537, 561, 674, 1084, 1093, 1409, 1442
BshVI ATCGAT 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 286
BsiWI CGTACG 1 cut(s) 345
BslI CCNNNNNNNGG 7 cut(s) 524, 670, 980, 1148, 1223, 1270, 1405
BsmAI GTCTC 1 cut(s) 1322
BsmI GAATGC 1 cut(s) 1575
BsnI GGCC 8 cut(s) 324, 537, 561, 674, 1084, 1093, 1409, 1442
Bsp1286I GDGCHC 1 cut(s) 286
Bsp1407I TGTACA 2 cut(s) 53, 1200
Bsp143I GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
Bsp1720I GCTNAGC 1 cut(s) 609
BspACI CCGC 5 cut(s) 14, 88, 196, 318, 1538
BspANI GGCC 8 cut(s) 324, 537, 561, 674, 1084, 1093, 1409, 1442
BspCNI CTCAG 2 cut(s) 1318, 1561
BspDI ATCGAT 1 cut(s) 402
BspLI GGNNCC 5 cut(s) 194, 735, 1092, 1214, 1441
BspPI GGATC 4 cut(s) 59, 390, 1434, 1538
BsrBI CCGCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 864
BsrGI TGTACA 2 cut(s) 53, 1200
BsrI ACTGG 3 cut(s) 737, 1443, 1524
BssECI CCNNGG 2 cut(s) 777, 1435
BssMI GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
BssT1I CCWWGG 2 cut(s) 777, 1435
Bst4CI ACNGT 9 cut(s) 111, 274, 523, 589, 629, 757, 1030, 1200, 1587
BstAUI TGTACA 2 cut(s) 53, 1200
BstBAI YACGTR 3 cut(s) 349, 820, 1526
BstC8I GCNNGC 1 cut(s) 697
BstDEI CTNAG 5 cut(s) 392, 542, 609, 1326, 1548
BstEII GGTNACC 1 cut(s) 1040
BstH2I RGCGCY 2 cut(s) 792, 827
BstHHI GCGC 2 cut(s) 791, 826
BstKTI GATC 6 cut(s) 67, 398, 726, 846, 1429, 1546
BstMAI GTCTC 1 cut(s) 1322
BstMBI GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
BstMWI GCNNNNNNNGC 4 cut(s) 179, 832, 1090, 1331
BstNSI RCATGY 2 cut(s) 385, 1495
BstPI GGTNACC 1 cut(s) 1040
BstSFI CTRYAG 3 cut(s) 792, 1420, 1586
BstSLI GKGCMC 1 cut(s) 286
BstSNI TACGTA 1 cut(s) 820
BstV1I GCAGC 3 cut(s) 77, 254, 1000
BstV2I GAAGAC 2 cut(s) 666, 1323
BstX2I RGATCY 4 cut(s) 64, 395, 843, 1426
BstXI CCANNNNNNTGG 2 cut(s) 545, 1052
BstYI RGATCY 4 cut(s) 64, 395, 843, 1426
Bsu15I ATCGAT 1 cut(s) 402
BsuRI GGCC 8 cut(s) 324, 537, 561, 674, 1084, 1093, 1409, 1442
BsuTUI ATCGAT 1 cut(s) 402
BtsIMutI CAGTG 3 cut(s) 594, 1026, 1583
Cac8I GCNNGC 1 cut(s) 697
CfoI GCGC 2 cut(s) 791, 826
Cfr13I GGNCC 5 cut(s) 560, 672, 774, 1091, 1440
ClaI ATCGAT 1 cut(s) 402
Csp6I GTAC 7 cut(s) 54, 112, 346, 630, 882, 1201, 1523
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviQI GTAC 7 cut(s) 54, 112, 346, 630, 882, 1201, 1523
DdeI CTNAG 5 cut(s) 392, 542, 609, 1326, 1548
DpnI GATC 6 cut(s) 66, 397, 725, 845, 1428, 1545
DpnII GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
DraI TTTAAA 2 cut(s) 166, 1501
EaeI YGGCCR 2 cut(s) 322, 535
Eco105I TACGTA 1 cut(s) 820
Eco130I CCWWGG 2 cut(s) 777, 1435
Eco147I AGGCCT 1 cut(s) 1409
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 774
Eco57I CTGAAG 3 cut(s) 45, 153, 870
Eco91I GGTNACC 1 cut(s) 1040
EcoO109I RGGNCCY 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 1040
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 2 cut(s) 777, 1435
ErhI CCWWGG 2 cut(s) 777, 1435
Fnu4HI GCNGC 3 cut(s) 91, 243, 989
Fsp4HI GCNGC 3 cut(s) 91, 243, 989
FspBI CTAG 2 cut(s) 614, 1190
GlaI GCGC 2 cut(s) 790, 825
GluI GCNGC 3 cut(s) 91, 243, 989
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 2 cut(s) 792, 827
HaeIII GGCC 8 cut(s) 324, 537, 561, 674, 1084, 1093, 1409, 1442
HhaI GCGC 2 cut(s) 791, 826
Hin6I GCGC 2 cut(s) 789, 824
HinP1I GCGC 2 cut(s) 789, 824
HindIII AAGCTT 1 cut(s) 697
HphI GGTGA 3 cut(s) 136, 244, 467
Hpy166II GTNNAC 4 cut(s) 284, 458, 592, 625
Hpy188I TCNGA 9 cut(s) 64, 133, 966, 1120, 1143, 1246, 1252, 1458, 1551
Hpy188III TCNNGA 8 cut(s) 638, 974, 1076, 1108, 1190, 1370, 1430, 1561
Hpy8I GTNNAC 4 cut(s) 284, 458, 592, 625
HpyAV CCTTC 1 cut(s) 1420
HpyCH4III ACNGT 9 cut(s) 111, 274, 523, 589, 629, 757, 1030, 1200, 1587
HpyCH4IV ACGT 3 cut(s) 348, 819, 1525
HpyCH4V TGCA 4 cut(s) 284, 695, 988, 1475
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 832, 1090, 1331
HpyF3I CTNAG 5 cut(s) 392, 542, 609, 1326, 1548
HpySE526I ACGT 3 cut(s) 348, 819, 1525
HspAI GCGC 2 cut(s) 789, 824
Kzo9I GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
Lsp1109I GCAGC 3 cut(s) 77, 254, 1000
LweI GCATC 2 cut(s) 595, 1164
MaeI CTAG 2 cut(s) 614, 1190
MaeII ACGT 3 cut(s) 348, 819, 1525
MaeIII GTNAC 3 cut(s) 412, 751, 1040
MalI GATC 6 cut(s) 66, 397, 725, 845, 1428, 1545
MbiI CCGCTC 1 cut(s) 16
MboI GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
MboII GAAGA 8 cut(s) 671, 733, 838, 883, 1064, 1096, 1239, 1328
MfeI CAATTG 1 cut(s) 1050
MflI RGATCY 4 cut(s) 64, 395, 843, 1426
MhlI GDGCHC 1 cut(s) 286
MlsI TGGCCA 2 cut(s) 324, 537
MluCI AATT 7 cut(s) 69, 633, 728, 967, 977, 1050, 1552
MluNI TGGCCA 2 cut(s) 324, 537
MlyI GAGTC 3 cut(s) 714, 1256, 1372
MmeI TCCRAC 2 cut(s) 767, 1230
MnlI CCTC 7 cut(s) 169, 328, 674, 677, 1275, 1399, 1557
Mox20I TGGCCA 2 cut(s) 324, 537
MroXI GAANNNNTTC 1 cut(s) 1556
MscI TGGCCA 2 cut(s) 324, 537
MseI TTAA 4 cut(s) 165, 408, 770, 1500
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 2 cut(s) 324, 537
MspA1I CMGCKG 1 cut(s) 90
MunI CAATTG 1 cut(s) 1050
Mva1269I GAATGC 1 cut(s) 1575
MwoI GCNNNNNNNGC 4 cut(s) 179, 832, 1090, 1331
NdeII GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
NlaIV GGNNCC 5 cut(s) 194, 735, 1092, 1214, 1441
NmuCI GTSAC 2 cut(s) 412, 751
NspI RCATGY 2 cut(s) 385, 1495
PceI AGGCCT 1 cut(s) 1409
PciI ACATGT 1 cut(s) 381
PctI GAATGC 1 cut(s) 1575
PdmI GAANNNNTTC 1 cut(s) 1556
PfeI GAWTC 7 cut(s) 254, 437, 961, 1022, 1138, 1157, 1291
Pfl23II CGTACG 1 cut(s) 345
PkrI GCNGC 3 cut(s) 92, 244, 990
PleI GAGTC 3 cut(s) 713, 1255, 1372
PpsI GAGTC 3 cut(s) 713, 1255, 1372
Ppu21I YACGTR 3 cut(s) 349, 820, 1526
PpuMI RGGWCCY 1 cut(s) 774
PscI ACATGT 1 cut(s) 381
PshBI ATTAAT 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 774
PspEI GGTNACC 1 cut(s) 1040
PspLI CGTACG 1 cut(s) 345
PspN4I GGNNCC 5 cut(s) 194, 735, 1092, 1214, 1441
PspPI GGNCC 5 cut(s) 560, 672, 774, 1091, 1440
PspPPI RGGWCCY 1 cut(s) 774
PsuI RGATCY 4 cut(s) 64, 395, 843, 1426
RsaI GTAC 7 cut(s) 55, 113, 347, 631, 883, 1202, 1524
RsaNI GTAC 7 cut(s) 54, 112, 346, 630, 882, 1201, 1523
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 4 cut(s) 165, 408, 770, 1500
SatI GCNGC 3 cut(s) 91, 243, 989
Sau3AI GATC 6 cut(s) 64, 395, 723, 843, 1426, 1543
Sau96I GGNCC 5 cut(s) 560, 672, 774, 1091, 1440
ScaI AGTACT 1 cut(s) 883
SchI GAGTC 3 cut(s) 714, 1256, 1372
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 2 cut(s) 595, 1164
SfcI CTRYAG 3 cut(s) 792, 1420, 1586
SfiI GGCCNNNNNGGCC 1 cut(s) 1090
SinI GGWCC 1 cut(s) 774
SmiMI CAYNNNNRTG 1 cut(s) 543
SnaBI TACGTA 1 cut(s) 820
Sse9I AATT 7 cut(s) 69, 633, 728, 967, 977, 1050, 1552
SseBI AGGCCT 1 cut(s) 1409
SsiI CCGC 5 cut(s) 14, 88, 196, 318, 1538
SspI AATATT 2 cut(s) 472, 490
SspMI CTAG 2 cut(s) 614, 1190
StuI AGGCCT 1 cut(s) 1409
StyI CCWWGG 2 cut(s) 777, 1435
TaaI ACNGT 9 cut(s) 111, 274, 523, 589, 629, 757, 1030, 1200, 1587
TaiI ACGT 3 cut(s) 351, 822, 1528
TaqI TCGA 3 cut(s) 269, 402, 435
TasI AATT 7 cut(s) 69, 633, 728, 967, 977, 1050, 1552
TatI WGTACW 3 cut(s) 53, 881, 1200
TfiI GAWTC 7 cut(s) 254, 437, 961, 1022, 1138, 1157, 1291
Tru1I TTAA 4 cut(s) 165, 408, 770, 1500
Tru9I TTAA 4 cut(s) 165, 408, 770, 1500
TscAI CASTG 3 cut(s) 594, 1033, 1590
TseFI GTSAC 2 cut(s) 412, 751
TseI GCWGC 3 cut(s) 90, 242, 988
Tsp45I GTSAC 2 cut(s) 412, 751
TspDTI ATGAA 7 cut(s) 267, 482, 838, 904, 942, 974, 1581
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 3 cut(s) 594, 1033, 1590
VneI GTGCAC 1 cut(s) 282
VpaK11BI GGWCC 1 cut(s) 774
VspI ATTAAT 1 cut(s) 408
XapI RAATTY 2 cut(s) 967, 1552
XbaI TCTAGA 1 cut(s) 1189
XceI RCATGY 2 cut(s) 385, 1495
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 1556
XspI CTAG 2 cut(s) 614, 1190
ZrmI AGTACT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.