MD05G1266700.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000956
Physical Location & Seq
Reverse (-)
40144255 .. 40145850
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1266700.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGAAGTTCCCAAGCTCTCCATTTCAACTCGCCATTCTCTCAATCCTTTCAGTCGTCACAGTTTCATGGGCAACTTCAGATCCACTTGTCAATATTGATGACTTTCTGCAATGCCTTTCTAAACAATCTCAGTCTACCAGCTCAATCCATGCAGGCATTTACACCCCTCAAAACACCTCGTTTCAATCTGTTCTGTCGGACCGTATTAAAAACCGCAGATTTTCTACATCCACAACTCCAAAACCTTTGGCCATTGTAGCAGCCAAGCACGAGTACCATGTTCAAGCAACTGTTCTATGTGCAAAACAGCAAGGATTGCAAATCAGAATCCGAAGTGGTGGCCATGATTACGAGGGTCTATCGTACGTGTCCCATGTACCCTTTATCATTCTTGACATGTTTCAACTCCAATCCATTAATGTTAATGTTGCGGAGAAGACCGCTTGGGTTCAGTCCGGAGCTACCCTTGGCCAAGTTTATTACAAAATTGCTGAGAAAAGCAACGTTCTAGGGTTTCCTGCTGGGGTTGGTCCAACTGTTGGTGCTGGTGGTCACTTTAGTGGCGGCGGGTATGGCTCTTTGATGCGTAAATATGGCCTTAGCATCGATAATATTATTGATGCTAAGATAGTTGTTGCAGATGGTAGAGTCCTTGATAGAAAATCAATGGGAGAAGATCTTTTCTGGGCTATTAGAGGAGGCGGTGGAGCTAGTTTCGGGGTCATTCTCTCGTGGAAGCTCAAGTTGGTTTCGGTTCCAGCTAAAGTGACTGTGTTCAACGTCTCAATAACCTTAGAGCAAGGGGCTACAGATGTCGTTTATAAATGGCAATACGCAGCACCCCTGCTGCCTAAAGACCTCTTCCTCAGAGTAAGGCCACTACAAGTGAACAGCACCGGGAGAAAGAAGACTGTGAAAGTATCATTCATTGGTCTTTTTTTGGGACGAACTAACAAGCTTCTTCCGTTGGTAAAGGAGCGCTTGCCCGAATTAGGTTTGCAACGAAGAGATTGTTTCGAAATGAGCTGGATTGAAAACATAGTGTTCTTTGCTGATTTCCCATTTGGAACTCCGATAACTACTCTTCTCAACAGGACAAATGGACAACCAAGCTTCTTCAAAGCCAAGTCTGACTATGTGACGAAACCGATTCCGAAACGAGTTATAGAATCCTTATGGAAGGAGATGATTAAGATAGACAATTTTTGGATGGACTGGAACCCTTACGGTGGAAGAATGGGCGAGATTCCTGAGTCGGCAACTCCGTCCCCTCACAGAGCTGGAAACCTATTTTTGATTCAATACTATGCGGTATGGGTAGAGGAAGGTGTTGAGGCAGCCAGCAAGTACCTTCAATTATCAAGAAAGTTGTTTGACCTGATGACTCCATATGTCTCCAAGAACCCAAGAGAGGCCTTCCAAAACTACCGGGATCTAGAAATTGGGGCTAATCTGGATAATCAGACTAACTTTCAGATTGCGAAGATTTATGGGAGCAAGTATTTTAAAGGTAATTTTGATAGACTGGTACGTGTGAAAACCATGGTTGATCCTCATAATTTTTTCAAGCATGAACAAAGTATCCCACCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

532

Amino Acids

59.46

Weight (kDa)

9.56

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 82 - 218 4.9e-27 FAD binding domain
BBE PF08031 472 - 529 1.7e-17 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 822
AccB7I CCANNNNNTGG 1 cut(s) 539
AccI GTMKAC 1 cut(s) 134
AccIII TCCGGA 1 cut(s) 455
AciI CCGC 7 cut(s) 214, 431, 441, 564, 567, 702, 1310
AclI AACGTT 1 cut(s) 504
AclWI GGATC 3 cut(s) 74, 1440, 1544
AcoI YGGCCR 3 cut(s) 249, 340, 469
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 5 cut(s) 275, 365, 378, 1349, 1530
AfeI AGCGCT 1 cut(s) 980
AfiI CCNNNNNNNGG 4 cut(s) 539, 992, 1229, 1411
AflIII ACRYGT 3 cut(s) 366, 396, 1531
AjuI GAANNNNNNNTTGG 2 cut(s) 728, 760
AleI CACNNNNGTG 1 cut(s) 558
AloI GAACNNNNNNTCC 1 cut(s) 1566
Alw26I GTCTC 2 cut(s) 787, 1399
AlwI GGATC 3 cut(s) 74, 1440, 1544
Aor13HI TCCGGA 1 cut(s) 455
Aor51HI AGCGCT 1 cut(s) 980
AoxI GGCC 6 cut(s) 249, 340, 469, 595, 875, 1413
ApeKI GCWGC 4 cut(s) 260, 836, 847, 1337
AseI ATTAAT 1 cut(s) 417
AspLEI GCGC 1 cut(s) 981
AspS9I GGNCC 2 cut(s) 199, 530
AsuC2I CCSGG 2 cut(s) 898, 1430
AsuII TTCGAA 1 cut(s) 1017
AvaII GGWCC 2 cut(s) 199, 530
BalI TGGCCA 3 cut(s) 251, 342, 471
BauI CACGAG 2 cut(s) 269, 730
BbsI GAAGAC 2 cut(s) 443, 914
BbvI GCAGC 4 cut(s) 272, 834, 848, 1349
BccI CCATC 2 cut(s) 635, 1204
BciVI GTATCC 1 cut(s) 1592
BcnI CCSGG 2 cut(s) 898, 1430
BcoDI GTCTC 2 cut(s) 787, 1399
BfaI CTAG 3 cut(s) 509, 711, 1436
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 1 cut(s) 982
BfuI GTATCC 1 cut(s) 1592
BglII AGATCT 1 cut(s) 676
BisI GCNGC 5 cut(s) 261, 565, 837, 848, 1338
BlsI GCNGC 5 cut(s) 262, 566, 838, 849, 1339
Bme1390I CCNGG 2 cut(s) 898, 1430
Bme18I GGWCC 2 cut(s) 199, 530
BmgT120I GGNCC 2 cut(s) 199, 530
BmiI GGNNCC 2 cut(s) 756, 1220
BmrFI CCNGG 2 cut(s) 898, 1430
BmsI GCATC 3 cut(s) 573, 610, 612
BpiI GAAGAC 2 cut(s) 443, 914
Bpu10I CCTNAGC 1 cut(s) 599
Bpu14I TTCGAA 1 cut(s) 1017
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 2 cut(s) 898, 1430
Bsa29I ATCGAT 1 cut(s) 606
BsaAI YACGTR 2 cut(s) 367, 1532
BsaJI CCNNGG 2 cut(s) 466, 1542
BsaWI WCCGGW 1 cut(s) 455
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 992, 1229, 1411
Bse1I ACTGG 2 cut(s) 1220, 1530
Bse3DI GCAATG 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 455
BseCI ATCGAT 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 466, 1542
BseGI GGATG 2 cut(s) 227, 1215
BseLI CCNNNNNNNGG 4 cut(s) 539, 992, 1229, 1411
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 4 cut(s) 143, 483, 880, 1242
BseNI ACTGG 2 cut(s) 1220, 1530
BseRI GAGGAG 1 cut(s) 711
BseXI GCAGC 4 cut(s) 272, 834, 848, 1349
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 6 cut(s) 251, 342, 471, 597, 877, 1415
BshVI ATCGAT 1 cut(s) 606
BsiSI CCGG 3 cut(s) 456, 897, 1429
BsiWI CGTACG 1 cut(s) 363
BslFI GGGAC 3 cut(s) 355, 957, 1252
BslI CCNNNNNNNGG 4 cut(s) 539, 992, 1229, 1411
BsmAI GTCTC 2 cut(s) 787, 1399
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 3 cut(s) 355, 957, 1252
BsnI GGCC 6 cut(s) 251, 342, 471, 597, 877, 1415
Bsp119I TTCGAA 1 cut(s) 1017
Bsp13I TCCGGA 1 cut(s) 455
Bsp143I GATC 4 cut(s) 79, 676, 1432, 1549
Bsp19I CCATGG 1 cut(s) 1542
BspACI CCGC 7 cut(s) 214, 431, 441, 564, 567, 702, 1310
BspANI GGCC 6 cut(s) 251, 342, 471, 597, 877, 1415
BspCNI CTCAG 4 cut(s) 142, 484, 879, 1243
BspDI ATCGAT 1 cut(s) 606
BspEI TCCGGA 1 cut(s) 455
BspLI GGNNCC 2 cut(s) 756, 1220
BspPI GGATC 3 cut(s) 74, 1440, 1544
BspT104I TTCGAA 1 cut(s) 1017
BsrDI GCAATG 1 cut(s) 116
BsrI ACTGG 2 cut(s) 1220, 1530
BssECI CCNNGG 2 cut(s) 466, 1542
BssMI GATC 4 cut(s) 79, 676, 1432, 1549
BssSI CACGAG 2 cut(s) 269, 730
BssT1I CCWWGG 2 cut(s) 466, 1542
Bst2BI CACGAG 2 cut(s) 269, 730
Bst4CI ACNGT 7 cut(s) 61, 203, 292, 538, 772, 913, 1229
Bst6I CTCTTC 3 cut(s) 866, 1000, 1089
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBAI YACGTR 2 cut(s) 367, 1532
BstBI TTCGAA 1 cut(s) 1017
BstC8I GCNNGC 3 cut(s) 154, 983, 1342
BstDEI CTNAG 7 cut(s) 129, 492, 599, 624, 793, 866, 1251
BstDSI CCRYGG 1 cut(s) 1542
BstF5I GGATG 2 cut(s) 227, 1215
BstH2I RGCGCY 1 cut(s) 982
BstHHI GCGC 1 cut(s) 981
BstKTI GATC 4 cut(s) 82, 679, 1435, 1552
BstMAI GTCTC 2 cut(s) 787, 1399
BstMBI GATC 4 cut(s) 79, 676, 1432, 1549
BstMWI GCNNNNNNNGC 3 cut(s) 257, 316, 573
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 896, 1428
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 4 cut(s) 272, 834, 848, 1349
BstV2I GAAGAC 2 cut(s) 443, 914
BstX2I RGATCY 3 cut(s) 79, 676, 1432
BstYI RGATCY 3 cut(s) 79, 676, 1432
Bsu15I ATCGAT 1 cut(s) 606
BsuI GTATCC 1 cut(s) 1592
BsuRI GGCC 6 cut(s) 251, 342, 471, 597, 877, 1415
BsuTUI ATCGAT 1 cut(s) 606
BtgI CCRYGG 1 cut(s) 1542
BtsCI GGATG 2 cut(s) 227, 1215
Cac8I GCNNGC 3 cut(s) 154, 983, 1342
CfoI GCGC 1 cut(s) 981
Cfr13I GGNCC 2 cut(s) 199, 530
ClaI ATCGAT 1 cut(s) 606
CpoI CGGWCCG 1 cut(s) 199
Csp6I GTAC 5 cut(s) 274, 364, 377, 1348, 1529
CspI CGGWCCG 1 cut(s) 199
CviAII CATG 8 cut(s) 66, 149, 278, 344, 374, 397, 1543, 1571
CviQI GTAC 5 cut(s) 274, 364, 377, 1348, 1529
DdeI CTNAG 7 cut(s) 129, 492, 599, 624, 793, 866, 1251
DpnI GATC 4 cut(s) 81, 678, 1434, 1551
DpnII GATC 4 cut(s) 79, 676, 1432, 1549
DraI TTTAAA 1 cut(s) 1507
EaeI YGGCCR 3 cut(s) 249, 340, 469
Eam1104I CTCTTC 3 cut(s) 866, 1000, 1089
EarI CTCTTC 3 cut(s) 866, 1000, 1089
Eco130I CCWWGG 2 cut(s) 466, 1542
Eco147I AGGCCT 1 cut(s) 1415
Eco47I GGWCC 2 cut(s) 199, 530
Eco47III AGCGCT 1 cut(s) 980
Eco57I CTGAAG 1 cut(s) 60
EcoT14I CCWWGG 2 cut(s) 466, 1542
ErhI CCWWGG 2 cut(s) 466, 1542
Esp3I CGTCTC 1 cut(s) 787
FaeI CATG 8 cut(s) 69, 152, 281, 347, 377, 400, 1546, 1574
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FaqI GGGAC 3 cut(s) 355, 957, 1252
FatI CATG 8 cut(s) 65, 148, 277, 343, 373, 396, 1542, 1570
FauI CCCGC 1 cut(s) 560
FauNDI CATATG 1 cut(s) 1390
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 5 cut(s) 261, 565, 837, 848, 1338
FokI GGATG 2 cut(s) 214, 1222
Fsp4HI GCNGC 5 cut(s) 261, 565, 837, 848, 1338
FspBI CTAG 3 cut(s) 509, 711, 1436
GlaI GCGC 1 cut(s) 980
GluI GCNGC 5 cut(s) 261, 565, 837, 848, 1338
GsaI CCCAGC 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 982
HaeIII GGCC 6 cut(s) 251, 342, 471, 597, 877, 1415
HapII CCGG 3 cut(s) 456, 897, 1429
HhaI GCGC 1 cut(s) 981
Hin1II CATG 8 cut(s) 69, 152, 281, 347, 377, 400, 1546, 1574
Hin6I GCGC 1 cut(s) 979
HinP1I GCGC 1 cut(s) 979
HindIII AAGCTT 2 cut(s) 956, 1111
HinfI GANTC 8 cut(s) 327, 648, 1150, 1169, 1246, 1253, 1297, 1384
HpaII CCGG 3 cut(s) 456, 897, 1429
Hpy166II GTNNAC 2 cut(s) 135, 889
Hpy188III TCNNGA 6 cut(s) 392, 456, 1250, 1362, 1436, 1454
Hpy8I GTNNAC 2 cut(s) 135, 889
HpyAV CCTTC 4 cut(s) 1174, 1319, 1361, 1426
HpyCH4III ACNGT 7 cut(s) 61, 203, 292, 538, 772, 913, 1229
HpyCH4IV ACGT 4 cut(s) 366, 504, 780, 1531
HpyCH4V TGCA 6 cut(s) 109, 152, 302, 319, 638, 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 316, 573
HpyF3I CTNAG 7 cut(s) 129, 492, 599, 624, 793, 866, 1251
HpySE526I ACGT 4 cut(s) 366, 504, 780, 1531
Hsp92II CATG 8 cut(s) 69, 152, 281, 347, 377, 400, 1546, 1574
HspAI GCGC 1 cut(s) 979
Kpn2I TCCGGA 1 cut(s) 455
Kzo9I GATC 4 cut(s) 79, 676, 1432, 1549
LmnI GCTCC 4 cut(s) 458, 707, 976, 1494
Lsp1109I GCAGC 4 cut(s) 272, 834, 848, 1349
LweI GCATC 3 cut(s) 573, 610, 612
MaeI CTAG 3 cut(s) 509, 711, 1436
MaeII ACGT 4 cut(s) 366, 504, 780, 1531
MaeIII GTNAC 4 cut(s) 55, 551, 766, 1138
MalI GATC 4 cut(s) 81, 678, 1434, 1551
MboI GATC 4 cut(s) 79, 676, 1432, 1549
MflI RGATCY 3 cut(s) 79, 676, 1432
MlsI TGGCCA 3 cut(s) 251, 342, 471
MluCI AATT 7 cut(s) 486, 989, 1201, 1355, 1440, 1513, 1558
MluNI TGGCCA 3 cut(s) 251, 342, 471
MlyI GAGTC 3 cut(s) 657, 1262, 1378
MmeI TCCRAC 2 cut(s) 177, 557
Mox20I TGGCCA 3 cut(s) 251, 342, 471
MroI TCCGGA 1 cut(s) 455
MscI TGGCCA 3 cut(s) 251, 342, 471
MseI TTAA 5 cut(s) 207, 417, 423, 1191, 1506
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 3 cut(s) 251, 342, 471
MspI CCGG 3 cut(s) 456, 897, 1429
MspR9I CCNGG 2 cut(s) 898, 1430
MwoI GCNNNNNNNGC 3 cut(s) 257, 316, 573
NciI CCSGG 2 cut(s) 898, 1430
NcoI CCATGG 1 cut(s) 1542
NdeI CATATG 1 cut(s) 1390
NdeII GATC 4 cut(s) 79, 676, 1432, 1549
NlaIII CATG 8 cut(s) 69, 152, 281, 347, 377, 400, 1546, 1574
NlaIV GGNNCC 2 cut(s) 756, 1220
NmuCI GTSAC 4 cut(s) 55, 551, 766, 1138
NspI RCATGY 1 cut(s) 400
NspV TTCGAA 1 cut(s) 1017
OliI CACNNNNGTG 1 cut(s) 558
PceI AGGCCT 1 cut(s) 1415
PciI ACATGT 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 1262
PfeI GAWTC 5 cut(s) 327, 1150, 1169, 1246, 1297
Pfl23II CGTACG 1 cut(s) 363
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 5 cut(s) 262, 566, 838, 849, 1339
PleI GAGTC 3 cut(s) 656, 1261, 1378
PpsI GAGTC 3 cut(s) 656, 1261, 1378
Ppu21I YACGTR 2 cut(s) 367, 1532
PscI ACATGT 1 cut(s) 396
PshBI ATTAAT 1 cut(s) 417
PsiI TTATAA 1 cut(s) 822
Psp1406I AACGTT 1 cut(s) 504
PspFI CCCAGC 1 cut(s) 521
PspLI CGTACG 1 cut(s) 363
PspN4I GGNNCC 2 cut(s) 756, 1220
PspPI GGNCC 2 cut(s) 199, 530
PsuI RGATCY 3 cut(s) 79, 676, 1432
RsaI GTAC 5 cut(s) 275, 365, 378, 1349, 1530
RsaNI GTAC 5 cut(s) 274, 364, 377, 1348, 1529
RseI CAYNNNNRTG 1 cut(s) 558
Rsr2I CGGWCCG 1 cut(s) 199
RsrII CGGWCCG 1 cut(s) 199
SaqAI TTAA 5 cut(s) 207, 417, 423, 1191, 1506
SatI GCNGC 5 cut(s) 261, 565, 837, 848, 1338
Sau3AI GATC 4 cut(s) 79, 676, 1432, 1549
Sau96I GGNCC 2 cut(s) 199, 530
SchI GAGTC 3 cut(s) 657, 1262, 1378
ScrFI CCNGG 2 cut(s) 898, 1430
SfaNI GCATC 3 cut(s) 573, 610, 612
SfcI CTRYAG 1 cut(s) 807
SfuI TTCGAA 1 cut(s) 1017
SinI GGWCC 2 cut(s) 199, 530
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 7 cut(s) 486, 989, 1201, 1355, 1440, 1513, 1558
SseBI AGGCCT 1 cut(s) 1415
SsiI CCGC 7 cut(s) 214, 431, 441, 564, 567, 702, 1310
SspI AATATT 2 cut(s) 94, 613
SspMI CTAG 3 cut(s) 509, 711, 1436
StuI AGGCCT 1 cut(s) 1415
StyD4I CCNGG 2 cut(s) 896, 1428
StyI CCWWGG 2 cut(s) 466, 1542
TaaI ACNGT 7 cut(s) 61, 203, 292, 538, 772, 913, 1229
TaiI ACGT 4 cut(s) 369, 507, 783, 1534
TaqI TCGA 2 cut(s) 606, 1017
TasI AATT 7 cut(s) 486, 989, 1201, 1355, 1440, 1513, 1558
TauI GCSGC 1 cut(s) 567
TfiI GAWTC 5 cut(s) 327, 1150, 1169, 1246, 1297
Tru1I TTAA 5 cut(s) 207, 417, 423, 1191, 1506
Tru9I TTAA 5 cut(s) 207, 417, 423, 1191, 1506
TseFI GTSAC 4 cut(s) 55, 551, 766, 1138
TseI GCWGC 4 cut(s) 260, 836, 847, 1337
Tsp45I GTSAC 4 cut(s) 55, 551, 766, 1138
TspDTI ATGAA 4 cut(s) 17, 54, 916, 1587
TspGWI ACGGA 2 cut(s) 954, 1254
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 2 cut(s) 199, 530
VspI ATTAAT 1 cut(s) 417
XbaI TCTAGA 1 cut(s) 1435
XceI RCATGY 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 134
XspI CTAG 3 cut(s) 509, 711, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.