Rroxscaffold_1G00059620

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000956
Physical Location & Seq
Forward (+)
81607064 .. 81608725
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059620.1

Sequence Viewer

Length: 1662 bp
ATGAAACAAACACTAATGAAGTTGCCGAGTTCTTTAGCAGTCGTTCTCTTCATTCTCTCAACCCTTTGGCTCTCAACTACTTCAAATCCCATTCTCGAAAACTTCCTCAAATGCCTTCCAGAACAAACTCACTCCAACTACTCCATCTCTGAAGCCATTTACACCCGCCAAAATGCCACTTTTGAATCCGTTCTGCTGTCATACATCAGAAACGGCAGATATTTAACCCCTACAACTCCGAAACCATTGGCTATTGTAACAGCCAAGCATGAATCCCACGTGCAAGCAACTGTGATCTGTGCAAAGCGGCATGGCTTGCAGATTAGAATCCGAAGTGGAGGCCACGATTACGAGGGTCTATCTTACGTGTCACATGTCCCCTTTGTTATTCTTGACATGTTTCATCTCCGCGCCATTCATGTCGATGTCGCGGATGAGAGTGCTTGGGTTGAGTCTGGGGCTATTGTTGGTGAGCTTTACTACGAAATCGCAAAGAACAGTAAAGTTCATGGGTTTTCAGCTGCAGCTGGTCCAACTGTCGGTGTTGGTGGTCACTTTAGTGGCGGTGGGTATGGTGTCATGATGAGAAAATATGGGCTCACCGTGGATAACATTGAAGATGCTAAACTAGTTAATGTGAATGGTACAGTTCTTGATAGAAAGTCTATGGGAGAAGATCTTTTTTGGGCCATTAGAGGTGGTGGTGGAGCAAGCTTTGGAGTCATTTTGTCATGGAAAATAAAGCTGCTTCCAGTGCCAGCCAAAGTGACTGTATTCAACATCGGAAGGACCCTTGAGCAAGGCGCTACTGATGTTCTTTACCGGTGGCAACACGTCGCACCTAAGCTACCTAAAGAAATCTTCATCAGAGCAATGCCTCGAGTGTTGAATACTAGTTTGGGTAACAAGACTGTGGAAGTGTCATTCATTGGTTTCTTCTTGGGACAAAGTGATGAGCTTCTTTCATTGATAAATAGAAGTCTTCCTGAATTGGGTTTGCAGAAAACAGATTGCTATGAAATGAGTTGGGTAGAATCTACGGTATTTTGGGCTAATTACTCGGTTGGAACTTCCATAGACGTTCTACTTGAAAGGCCCAAGGGGCCAACAGACAATTTCAAAGGCAAGTCTGACTATGTGAAAGAACCAATTCCAAAACAAGACATAGAATTCATATTGAAGGAGCTACTTAAGGTGGAACACTTGTGGATGCAATGGAACCCTTATGGTGGAAGAATGAGTGAGATTTCTGAGTCGGAAACTCCCTTCCCTCACAGAGCCGGAAACCTGTTCTTGATTCAATACTCTTCGTCGTGGGAGGAAGAAGGGATTGAGGCCACCAACAAGTACCTTGGCTTATCTAGAAAATTTTACAAAATAATGACTCCATTTGTATCCAAGAGGCCAAGAGAGGCCTTCCAAAACTACAGGGATCTTGACATTGGGGCCAATTTGGATAATCAGACCATCTTTAATATTGCTAGAGTCTATGGAAGCAAATACTTTGAAGGTAATTTTGAAAGGCTGGTACATGTGAAGACCGAGGTTGATCCCCAAAACTTTTTCAAACATGAACAAAGTATTCCACCTCTTGTCCAGGGAAAATTCTACAATGTGTTAACGTATGACATGCATACAATTGCCTTAAAAGTGGTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

553

Amino Acids

62.35

Weight (kDa)

8.42

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 82 - 218 2e-26 FAD binding domain
BBE PF08031 472 - 529 1.1e-17 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 411, 431
AciI CCGC 5 cut(s) 166, 307, 409, 431, 564
AclWI GGATC 2 cut(s) 1440, 1544
AcsI RAATTY 3 cut(s) 1169, 1367, 1604
AcuI CTGAAG 1 cut(s) 171
AcvI CACGTG 1 cut(s) 280
AfaI GTAC 3 cut(s) 646, 1349, 1530
AfiI CCNNNNNNNGG 3 cut(s) 539, 992, 1229
AflII CTTAAG 1 cut(s) 1190
AflIII ACRYGT 5 cut(s) 366, 373, 396, 832, 1531
AgeI ACCGGT 1 cut(s) 822
AhlI ACTAGT 2 cut(s) 628, 893
AjiI CACGTC 1 cut(s) 835
AjnI CCWGG 1 cut(s) 1596
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AleI CACNNNNGTG 1 cut(s) 558
AluBI AGCT 8 cut(s) 475, 521, 527, 714, 745, 847, 958, 1186
AluI AGCT 8 cut(s) 475, 521, 527, 714, 745, 847, 958, 1186
AlwI GGATC 2 cut(s) 1440, 1544
Ama87I CYCGRG 1 cut(s) 879
AoxI GGCC 8 cut(s) 340, 687, 1094, 1103, 1335, 1403, 1413, 1446
ApeKI GCWGC 3 cut(s) 521, 524, 745
ApoI RAATTY 3 cut(s) 1169, 1367, 1604
AsiGI ACCGGT 1 cut(s) 822
Asp700I GAANNNNTTC 2 cut(s) 189, 1149
AspLEI GCGC 2 cut(s) 413, 806
AspS9I GGNCC 6 cut(s) 530, 687, 789, 1095, 1103, 1446
AsuHPI GGTGA 2 cut(s) 482, 592
AvaI CYCGRG 1 cut(s) 879
AvaII GGWCC 2 cut(s) 530, 789
BanII GRGCYC 1 cut(s) 600
BbrPI CACGTG 1 cut(s) 280
BbsI GAAGAC 2 cut(s) 974, 1544
BbvI GCAGC 3 cut(s) 508, 536, 732
BccI CCATC 2 cut(s) 152, 1475
BceAI ACGGC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 1598
BciVI GTATCC 1 cut(s) 1405
BcuI ACTAGT 2 cut(s) 628, 893
BfaI CTAG 4 cut(s) 629, 894, 1362, 1482
BfmI CTRYAG 2 cut(s) 522, 1426
BfoI RGCGCY 1 cut(s) 807
BfrI CTTAAG 1 cut(s) 1190
BfuI GTATCC 1 cut(s) 1405
BglI GCCNNNNNGGC 1 cut(s) 1102
BglII AGATCT 1 cut(s) 676
BisI GCNGC 4 cut(s) 308, 522, 525, 746
BlsI GCNGC 4 cut(s) 309, 523, 526, 747
Bme1390I CCNGG 1 cut(s) 1598
Bme18I GGWCC 2 cut(s) 530, 789
BmeT110I CYCGRG 1 cut(s) 879
BmgBI CACGTC 1 cut(s) 835
BmgT120I GGNCC 6 cut(s) 530, 687, 789, 1095, 1103, 1446
BmiI GGNNCC 4 cut(s) 791, 1104, 1220, 1447
BmrFI CCNGG 1 cut(s) 1598
BmsI GCATC 2 cut(s) 610, 1200
BpiI GAAGAC 2 cut(s) 974, 1544
Bpu10I CCTNAGC 1 cut(s) 843
BpuEI CTTGAG 1 cut(s) 815
BsaAI YACGTR 2 cut(s) 280, 367
BsaBI GATNNNNATC 1 cut(s) 326
BsaJI CCNNGG 5 cut(s) 603, 1098, 1351, 1542, 1597
BsaWI WCCGGW 1 cut(s) 822
Bsc4I CCNNNNNNNGG 3 cut(s) 539, 992, 1229
Bse118I RCCGGY 1 cut(s) 822
Bse1I ACTGG 1 cut(s) 752
Bse3DI GCAATG 2 cut(s) 879, 1220
Bse8I GATNNNNATC 1 cut(s) 326
BseBI CCWGG 1 cut(s) 1598
BseDI CCNNGG 5 cut(s) 603, 1098, 1351, 1542, 1597
BseGI GGATG 2 cut(s) 439, 1215
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 3 cut(s) 539, 992, 1229
BseMI GCAATG 2 cut(s) 879, 1220
BseMII CTCAG 1 cut(s) 1242
BseNI ACTGG 1 cut(s) 752
BseXI GCAGC 3 cut(s) 508, 536, 732
Bsh1236I CGCG 2 cut(s) 411, 431
BshFI GGCC 8 cut(s) 342, 689, 1096, 1105, 1337, 1405, 1415, 1448
BshTI ACCGGT 1 cut(s) 822
BsiHKCI CYCGRG 1 cut(s) 879
BsiSI CCGG 2 cut(s) 823, 1281
BslFI GGGAC 2 cut(s) 362, 957
BslI CCNNNNNNNGG 3 cut(s) 539, 992, 1229
BsmFI GGGAC 2 cut(s) 362, 957
BsnI GGCC 8 cut(s) 342, 689, 1096, 1105, 1337, 1405, 1415, 1448
BsoBI CYCGRG 1 cut(s) 879
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 4 cut(s) 294, 676, 1432, 1549
BspACI CCGC 5 cut(s) 166, 307, 409, 431, 564
BspANI GGCC 8 cut(s) 342, 689, 1096, 1105, 1337, 1405, 1415, 1448
BspCNI CTCAG 1 cut(s) 1243
BspFNI CGCG 2 cut(s) 411, 431
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 4 cut(s) 791, 1104, 1220, 1447
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 2 cut(s) 1440, 1544
BspTI CTTAAG 1 cut(s) 1190
BsrDI GCAATG 2 cut(s) 879, 1220
BsrFI RCCGGY 1 cut(s) 822
BsrI ACTGG 1 cut(s) 752
BssAI RCCGGY 1 cut(s) 822
BssECI CCNNGG 5 cut(s) 603, 1098, 1351, 1542, 1597
BssMI GATC 4 cut(s) 294, 676, 1432, 1549
BssT1I CCWWGG 2 cut(s) 1098, 1351
Bst2UI CCWGG 1 cut(s) 1598
Bst4CI ACNGT 8 cut(s) 292, 500, 538, 604, 649, 772, 913, 1042
Bst6I CTCTTC 2 cut(s) 53, 1312
BstAFI CTTAAG 1 cut(s) 1190
BstAPI GCANNNNNTGC 1 cut(s) 316
BstBAI YACGTR 2 cut(s) 280, 367
BstC8I GCNNGC 4 cut(s) 285, 317, 712, 759
BstDEI CTNAG 2 cut(s) 843, 1251
BstDSI CCRYGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 439, 1215
BstFNI CGCG 2 cut(s) 411, 431
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 2 cut(s) 413, 806
BstKTI GATC 4 cut(s) 297, 679, 1435, 1552
BstMBI GATC 4 cut(s) 294, 676, 1432, 1549
BstMWI GCNNNNNNNGC 3 cut(s) 316, 754, 1102
BstNI CCWGG 1 cut(s) 1598
BstNSI RCATGY 4 cut(s) 377, 400, 1535, 1633
BstSCI CCNGG 1 cut(s) 1596
BstSFI CTRYAG 2 cut(s) 522, 1426
BstUI CGCG 2 cut(s) 411, 431
BstV1I GCAGC 3 cut(s) 508, 536, 732
BstV2I GAAGAC 2 cut(s) 974, 1544
BstX2I RGATCY 2 cut(s) 676, 1432
BstYI RGATCY 2 cut(s) 676, 1432
BsuI GTATCC 1 cut(s) 1405
BsuRI GGCC 8 cut(s) 342, 689, 1096, 1105, 1337, 1405, 1415, 1448
BtgI CCRYGG 1 cut(s) 603
BtrI CACGTC 1 cut(s) 835
BtsCI GGATG 2 cut(s) 439, 1215
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 4 cut(s) 285, 317, 712, 759
CciI TCATGA 1 cut(s) 579
CfoI GCGC 2 cut(s) 413, 806
Cfr10I RCCGGY 1 cut(s) 822
Cfr13I GGNCC 6 cut(s) 530, 687, 789, 1095, 1103, 1446
Csp6I GTAC 3 cut(s) 645, 1348, 1529
CspAI ACCGGT 1 cut(s) 822
CviQI GTAC 3 cut(s) 645, 1348, 1529
DdeI CTNAG 2 cut(s) 843, 1251
DpnI GATC 4 cut(s) 296, 678, 1434, 1551
DpnII GATC 4 cut(s) 294, 676, 1432, 1549
Eam1104I CTCTTC 2 cut(s) 53, 1312
EarI CTCTTC 2 cut(s) 53, 1312
Eco130I CCWWGG 2 cut(s) 1098, 1351
Eco147I AGGCCT 1 cut(s) 1415
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 530, 789
Eco57I CTGAAG 1 cut(s) 171
Eco72I CACGTG 1 cut(s) 280
Eco88I CYCGRG 1 cut(s) 879
EcoO109I RGGNCCY 1 cut(s) 789
EcoRI GAATTC 1 cut(s) 1169
EcoRII CCWGG 1 cut(s) 1596
EcoT14I CCWWGG 2 cut(s) 1098, 1351
EcoT22I ATGCAT 1 cut(s) 1635
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 2 cut(s) 1098, 1351
FaqI GGGAC 2 cut(s) 362, 957
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 4 cut(s) 308, 522, 525, 746
FokI GGATG 2 cut(s) 446, 1222
FriOI GRGCYC 1 cut(s) 600
Fsp4HI GCNGC 4 cut(s) 308, 522, 525, 746
FspBI CTAG 4 cut(s) 629, 894, 1362, 1482
GlaI GCGC 2 cut(s) 412, 805
GluI GCNGC 4 cut(s) 308, 522, 525, 746
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 8 cut(s) 342, 689, 1096, 1105, 1337, 1405, 1415, 1448
HapII CCGG 2 cut(s) 823, 1281
HhaI GCGC 2 cut(s) 413, 806
Hin6I GCGC 2 cut(s) 411, 804
HinP1I GCGC 2 cut(s) 411, 804
HincII GTYRAC 1 cut(s) 1620
HindII GTYRAC 1 cut(s) 1620
HindIII AAGCTT 1 cut(s) 712
HpaI GTTAAC 1 cut(s) 1620
HpaII CCGG 2 cut(s) 823, 1281
HphI GGTGA 2 cut(s) 482, 592
Hpy166II GTNNAC 1 cut(s) 1620
Hpy188III TCNNGA 9 cut(s) 95, 119, 392, 580, 653, 986, 1294, 1362, 1436
Hpy8I GTNNAC 1 cut(s) 1620
Hpy99I CGWCG 2 cut(s) 839, 1315
HpyAV CCTTC 7 cut(s) 125, 780, 1174, 1276, 1319, 1426, 1502
HpyCH4III ACNGT 8 cut(s) 292, 500, 538, 604, 649, 772, 913, 1042
HpyCH4IV ACGT 5 cut(s) 279, 366, 834, 1080, 1622
HpyCH4V TGCA 7 cut(s) 283, 302, 319, 524, 1000, 1213, 1633
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 754, 1102
HpyF3I CTNAG 2 cut(s) 843, 1251
HpySE526I ACGT 5 cut(s) 279, 366, 834, 1080, 1622
HspAI GCGC 2 cut(s) 411, 804
KspAI GTTAAC 1 cut(s) 1620
Kzo9I GATC 4 cut(s) 294, 676, 1432, 1549
LmnI GCTCC 2 cut(s) 707, 1183
Lsp1109I GCAGC 3 cut(s) 508, 536, 732
LweI GCATC 2 cut(s) 610, 1200
MaeI CTAG 4 cut(s) 629, 894, 1362, 1482
MaeII ACGT 5 cut(s) 279, 366, 834, 1080, 1622
MaeIII GTNAC 5 cut(s) 256, 369, 551, 766, 902
MalI GATC 4 cut(s) 296, 678, 1434, 1551
MboI GATC 4 cut(s) 294, 676, 1432, 1549
MfeI CAATTG 1 cut(s) 1638
MflI RGATCY 2 cut(s) 676, 1432
MhlI GDGCHC 1 cut(s) 600
MlyI GAGTC 5 cut(s) 461, 729, 1262, 1378, 1494
MmeI TCCRAC 4 cut(s) 159, 557, 1045, 1236
Mph1103I ATGCAT 1 cut(s) 1635
MroXI GAANNNNTTC 2 cut(s) 189, 1149
MseI TTAA 6 cut(s) 224, 633, 1191, 1473, 1619, 1646
MslI CAYNNNNRTG 2 cut(s) 423, 558
MspA1I CMGCKG 2 cut(s) 521, 527
MspCI CTTAAG 1 cut(s) 1190
MspI CCGG 2 cut(s) 823, 1281
MspR9I CCNGG 1 cut(s) 1598
MunI CAATTG 1 cut(s) 1638
MvaI CCWGG 1 cut(s) 1598
MvnI CGCG 2 cut(s) 411, 431
MwoI GCNNNNNNNGC 3 cut(s) 316, 754, 1102
NdeII GATC 4 cut(s) 294, 676, 1432, 1549
NlaIV GGNNCC 4 cut(s) 791, 1104, 1220, 1447
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 3 cut(s) 369, 551, 766
NsiI ATGCAT 1 cut(s) 1635
NspI RCATGY 4 cut(s) 377, 400, 1535, 1633
OliI CACNNNNGTG 1 cut(s) 558
PaeR7I CTCGAG 1 cut(s) 879
PagI TCATGA 1 cut(s) 579
PceI AGGCCT 1 cut(s) 1415
PciI ACATGT 3 cut(s) 373, 396, 1531
PdmI GAANNNNTTC 2 cut(s) 189, 1149
PfeI GAWTC 5 cut(s) 185, 272, 327, 1034, 1297
PinAI ACCGGT 1 cut(s) 822
PkrI GCNGC 4 cut(s) 309, 523, 526, 747
PleI GAGTC 5 cut(s) 460, 728, 1261, 1378, 1493
PmaCI CACGTG 1 cut(s) 280
PmlI CACGTG 1 cut(s) 280
PpsI GAGTC 5 cut(s) 460, 728, 1261, 1378, 1493
Ppu21I YACGTR 2 cut(s) 280, 367
PpuMI RGGWCCY 1 cut(s) 789
PscI ACATGT 3 cut(s) 373, 396, 1531
Psp5II RGGWCCY 1 cut(s) 789
Psp6I CCWGG 1 cut(s) 1596
PspCI CACGTG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 1596
PspN4I GGNNCC 4 cut(s) 791, 1104, 1220, 1447
PspPI GGNCC 6 cut(s) 530, 687, 789, 1095, 1103, 1446
PspPPI RGGWCCY 1 cut(s) 789
PspXI VCTCGAGB 1 cut(s) 879
PstI CTGCAG 1 cut(s) 526
PsuI RGATCY 2 cut(s) 676, 1432
PvuII CAGCTG 2 cut(s) 521, 527
RsaI GTAC 3 cut(s) 646, 1349, 1530
RsaNI GTAC 3 cut(s) 645, 1348, 1529
RseI CAYNNNNRTG 2 cut(s) 423, 558
SaqAI TTAA 6 cut(s) 224, 633, 1191, 1473, 1619, 1646
SatI GCNGC 4 cut(s) 308, 522, 525, 746
Sau3AI GATC 4 cut(s) 294, 676, 1432, 1549
Sau96I GGNCC 6 cut(s) 530, 687, 789, 1095, 1103, 1446
SchI GAGTC 5 cut(s) 461, 729, 1262, 1378, 1494
ScrFI CCNGG 1 cut(s) 1598
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 2 cut(s) 610, 1200
SfcI CTRYAG 2 cut(s) 522, 1426
SfiI GGCCNNNNNGGCC 1 cut(s) 1102
Sfr274I CTCGAG 1 cut(s) 879
SinI GGWCC 2 cut(s) 530, 789
SlaI CTCGAG 1 cut(s) 879
SmiMI CAYNNNNRTG 2 cut(s) 423, 558
SmlI CTYRAG 3 cut(s) 794, 879, 1190
SmoI CTYRAG 3 cut(s) 794, 879, 1190
SpeI ACTAGT 2 cut(s) 628, 893
SseBI AGGCCT 1 cut(s) 1415
SsiI CCGC 5 cut(s) 166, 307, 409, 431, 564
SspI AATATT 1 cut(s) 1477
SspMI CTAG 4 cut(s) 629, 894, 1362, 1482
StuI AGGCCT 1 cut(s) 1415
StyD4I CCNGG 1 cut(s) 1596
StyI CCWWGG 2 cut(s) 1098, 1351
TaaI ACNGT 8 cut(s) 292, 500, 538, 604, 649, 772, 913, 1042
TaiI ACGT 5 cut(s) 282, 369, 837, 1083, 1625
TaqI TCGA 3 cut(s) 96, 423, 880
TaqII GACCGA 1 cut(s) 1556
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 5 cut(s) 185, 272, 327, 1034, 1297
Tru1I TTAA 6 cut(s) 224, 633, 1191, 1473, 1619, 1646
Tru9I TTAA 6 cut(s) 224, 633, 1191, 1473, 1619, 1646
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 3 cut(s) 369, 551, 766
TseI GCWGC 3 cut(s) 521, 524, 745
Tsp45I GTSAC 3 cut(s) 369, 551, 766
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 1 cut(s) 759
Vha464I CTTAAG 1 cut(s) 1190
VpaK11BI GGWCC 2 cut(s) 530, 789
XapI RAATTY 3 cut(s) 1169, 1367, 1604
XbaI TCTAGA 1 cut(s) 1361
XceI RCATGY 4 cut(s) 377, 400, 1535, 1633
XhoI CTCGAG 1 cut(s) 879
XmnI GAANNNNTTC 2 cut(s) 189, 1149
XspI CTAG 4 cut(s) 629, 894, 1362, 1482
Zsp2I ATGCAT 1 cut(s) 1635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.