Prupe.4G098600_v2.0.a1

Berberine and berberine like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000956
Physical Location & Seq
Forward (+)
5007238 .. 5009110
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G098600.1

Sequence Viewer

Length: 1593 bp
ATGAAGTTCCGAAACCCTAATGTACTTCTTTTCATTCTCTCAGTCCTTTCAATCTCAGTATCATGGGCAACTTCAGCAACAGCTGACTTTCATGGCTTTCTCCAATGCCTTACCAAACGTTCTCACTCTGCTCACCCAATCCAGGAAACAATTTACACCCCTCAAAATGCCTCCTTTCAATCTGTTTTGGTATTACATATCAACAACCGCAGATTTTCTACACCAACAACTCCAAAACCTTTGGCCATAATAGCAGCCAAGAATGAATCCCATGTCCAAGCAACTGTGGTTTGTGCCAAACGTCATGGATTGCAAATTAAAATCCGAAGTGGTGGCCACGATTTCGAGGGTTTATCATACACGTCGGATGTCCCTTTTGTCATTCTTGACATGTTTAATCTTAAATCCGTTGATGTTAATCTTGCGCAGAAAAGAGCTTGGGTTATGTCTGGAGCTACTCTTGGAGAAGTTTATTATGCAATTGCTGCAAAAACCAAGGTTTACGGGTTCCCTGCTGGGATTTGTCCAACGGTTGGTGCTGGAGGCCACTTCGGGGGTGGCGGGTATGGGTTTATGATGAGAAAATATGGGCTTAGCGTCGATAATATCGTTGATGCTAAGATAGTTACTGTGAAGGGTACAATCCTTGACAGAAAGTCAATGGGAGAAGACCTTTTCTGGGCCATCAGAGGAGGTGGTGGAGCTAGCTTCGGAGTCATTCTTTCATGGAAGCTCAAGCTGGTTCCGGTTCCAGCCAAAGTGACTGTGTTCAACGTTACAAGAACCATAGAGGAAGGTGCTACAGATCTTGTTTATAAGTGGCAAACTGTTGCACCCAAGCTGCCCAAAGAGCTCTTCCTCAGGGCAATGCCACAAGTGAAGAATATTGATACCAAGGGAAAAAAGACTGTGGCAGTATCGTTCCTTGGCCATTTCTTGGGAAAAAGTGACAAGGTTGTTGCATTGCTGAATGAGCGTTTCCCTGAATTGGGTTTACAACGAAGAGACTGCCATGAAGTGAGTTGGGTGGAATCCACTGTATTTTGGGCTGACAATCCTATTGGAACTCCGATAAATGTTCTTCTCAACAAGCCAACTGAACCTGAAACCTTCTACAAAGGTAAGTCTGACTATGTAAAGAAGCCACTTCCAAAACATGTTTTTGAATCTATATGGAAGAAGATGATCGAGATTGAGAATATTTGGTTGGATTGGAACCCTTACGGTGGAAGAATGAATGAGATTTCTGTGTCAGCAACTCCATACCCTCACAGGGCCGGAAATCTATTTTTTGCTCTATACTATTCGTCGTGGTATGATGAAGGGATTGAGACCACCAACAAGTATCTTGGCTTAATAAGAGAGTTGTACGAAATGATGACTCCATATGTCTCCGAGAATCCAAGAGAGGCCTTCCAAAACTACAGGGATCTCGACATTGGTGCCAATCAGGACAGTCAGACTAACTTTGAGACGGCTAAACTTTATGGAAAGAAGTACTTTAAAGGTAATTTTGATAGATTGGTACGTGTGAAAACCAAGGTGGATCCTCATAATTTTTTCAAGCATAAACAAAGTATCCCAACTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

531

Amino Acids

59.62

Weight (kDa)

9.45

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
Acc16I TGCGCA 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 1442
AccB7I CCANNNNNTGG 2 cut(s) 533, 937
AciI CCGC 2 cut(s) 208, 561
AclI AACGTT 2 cut(s) 118, 774
AclWI GGATC 3 cut(s) 1437, 1541, 1554
AcoI YGGCCR 3 cut(s) 243, 334, 928
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 5 cut(s) 24, 640, 1370, 1499, 1527
AfiI CCNNNNNNNGG 9 cut(s) 142, 533, 553, 679, 937, 988, 989, 1226, 1273
AflIII ACRYGT 4 cut(s) 360, 390, 1156, 1528
AgsI TTSAA 5 cut(s) 51, 179, 772, 1166, 1564
AhdI GACNNNNNGTC 1 cut(s) 655
AjiI CACGTC 1 cut(s) 363
AjnI CCWGG 1 cut(s) 141
AjuI GAANNNNNNNTTGG 4 cut(s) 839, 871, 1190, 1222
AluBI AGCT 9 cut(s) 83, 437, 455, 704, 708, 733, 739, 841, 853
AluI AGCT 9 cut(s) 83, 437, 455, 704, 708, 733, 739, 841, 853
Alw21I GWGCWC 1 cut(s) 855
Alw26I GTCTC 4 cut(s) 999, 1325, 1396, 1466
AlwI GGATC 3 cut(s) 1437, 1541, 1554
AoxI GGCC 7 cut(s) 243, 334, 544, 681, 928, 1275, 1410
ApeKI GCWGC 3 cut(s) 254, 485, 841
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 681, 1275
AsuHPI GGTGA 1 cut(s) 125
AsuNHI GCTAGC 1 cut(s) 704
AxyI CCTNAGG 1 cut(s) 860
BalI TGGCCA 3 cut(s) 245, 336, 930
BamHI GGATCC 1 cut(s) 1546
BanI GGYRCC 1 cut(s) 1442
BanII GRGCYC 1 cut(s) 855
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 855
BbvI GCAGC 3 cut(s) 266, 472, 828
BccI CCATC 1 cut(s) 692
BceAI ACGGC 1 cut(s) 1491
BcgI CGANNNNNNTGC 4 cut(s) 990, 1024, 1424, 1458
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 1 cut(s) 1589
BcoDI GTCTC 4 cut(s) 999, 1325, 1396, 1466
BfaI CTAG 1 cut(s) 705
BfmI CTRYAG 2 cut(s) 801, 1423
BfuI GTATCC 1 cut(s) 1589
BglII AGATCT 1 cut(s) 805
BisI GCNGC 3 cut(s) 255, 486, 842
BlpI GCTNAGC 1 cut(s) 593
BlsI GCNGC 3 cut(s) 256, 487, 843
BmcAI AGTACT 1 cut(s) 1499
Bme1390I CCNGG 1 cut(s) 143
BmeRI GACNNNNNGTC 1 cut(s) 655
BmgBI CACGTC 1 cut(s) 363
BmgT120I GGNCC 2 cut(s) 681, 1275
BmiI GGNNCC 6 cut(s) 509, 744, 750, 1217, 1444, 1548
BmrFI CCNGG 1 cut(s) 143
BmsI GCATC 1 cut(s) 604
BmtI GCTAGC 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 675
BpmI CTGGAG 2 cut(s) 471, 561
Bpu1102I GCTNAGC 1 cut(s) 593
BpuEI CTTGAG 1 cut(s) 719
BsaAI YACGTR 1 cut(s) 1529
BsaBI GATNNNNATC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 1325
BsaJI CCNNGG 4 cut(s) 495, 894, 925, 1539
BsaWI WCCGGW 1 cut(s) 745
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 533, 553, 679, 937, 988, 989, 1226, 1273
Bse21I CCTNAGG 1 cut(s) 860
Bse3DI GCAATG 2 cut(s) 873, 962
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 4 cut(s) 495, 894, 925, 1539
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 9 cut(s) 142, 533, 553, 679, 937, 988, 989, 1226, 1273
BseMI GCAATG 2 cut(s) 873, 962
BseMII CTCAG 3 cut(s) 54, 69, 874
BseRI GAGGAG 1 cut(s) 705
BseXI GCAGC 3 cut(s) 266, 472, 828
BseYI CCCAGC 1 cut(s) 515
BshFI GGCC 7 cut(s) 245, 336, 546, 683, 930, 1277, 1412
BshNI GGYRCC 1 cut(s) 1442
BsiHKAI GWGCWC 1 cut(s) 855
BsiSI CCGG 2 cut(s) 746, 1278
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 9 cut(s) 142, 533, 553, 679, 937, 988, 989, 1226, 1273
BsmAI GTCTC 4 cut(s) 999, 1325, 1396, 1466
BsmBI CGTCTC 1 cut(s) 1466
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 7 cut(s) 245, 336, 546, 683, 930, 1277, 1412
Bso31I GGTCTC 1 cut(s) 1325
Bsp1286I GDGCHC 1 cut(s) 855
Bsp143I GATC 4 cut(s) 805, 1185, 1429, 1546
Bsp1720I GCTNAGC 1 cut(s) 593
BspACI CCGC 2 cut(s) 208, 561
BspANI GGCC 7 cut(s) 245, 336, 546, 683, 930, 1277, 1412
BspCNI CTCAG 3 cut(s) 53, 68, 873
BspLI GGNNCC 6 cut(s) 509, 744, 750, 1217, 1444, 1548
BspOI GCTAGC 1 cut(s) 708
BspPI GGATC 3 cut(s) 1437, 1541, 1554
BspQI GCTCTTC 1 cut(s) 860
BspT107I GGYRCC 1 cut(s) 1442
BspTNI GGTCTC 1 cut(s) 1325
BsrDI GCAATG 2 cut(s) 873, 962
BssECI CCNNGG 4 cut(s) 495, 894, 925, 1539
BssMI GATC 4 cut(s) 805, 1185, 1429, 1546
BssT1I CCWWGG 4 cut(s) 495, 894, 925, 1539
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 9 cut(s) 286, 532, 631, 766, 829, 910, 1039, 1226, 1457
Bst6I CTCTTC 2 cut(s) 860, 997
BstAPI GCANNNNNTGC 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 1529
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 5 cut(s) 40, 55, 593, 618, 860
BstF5I GGATG 1 cut(s) 373
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 4 cut(s) 808, 1188, 1432, 1549
BstMAI GTCTC 4 cut(s) 999, 1325, 1396, 1466
BstMBI GATC 4 cut(s) 805, 1185, 1429, 1546
BstMWI GCNNNNNNNGC 5 cut(s) 74, 251, 485, 850, 973
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 2 cut(s) 394, 1160
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 2 cut(s) 801, 1423
BstV1I GCAGC 3 cut(s) 266, 472, 828
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 3 cut(s) 805, 1429, 1546
BstYI RGATCY 3 cut(s) 805, 1429, 1546
Bsu36I CCTNAGG 1 cut(s) 860
BsuI GTATCC 1 cut(s) 1589
BsuRI GGCC 7 cut(s) 245, 336, 546, 683, 930, 1277, 1412
BtrI CACGTC 1 cut(s) 363
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 1035
Cac8I GCNNGC 1 cut(s) 706
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 2 cut(s) 681, 1275
CseI GACGC 1 cut(s) 586
Csp6I GTAC 5 cut(s) 23, 639, 1369, 1498, 1526
CviAII CATG 8 cut(s) 63, 92, 272, 305, 391, 726, 1013, 1157
CviQI GTAC 5 cut(s) 23, 639, 1369, 1498, 1526
DdeI CTNAG 5 cut(s) 40, 55, 593, 618, 860
DpnI GATC 4 cut(s) 807, 1187, 1431, 1548
DpnII GATC 4 cut(s) 805, 1185, 1429, 1546
DraI TTTAAA 1 cut(s) 1504
DriI GACNNNNNGTC 1 cut(s) 655
EaeI YGGCCR 3 cut(s) 243, 334, 928
Eam1104I CTCTTC 2 cut(s) 860, 997
Eam1105I GACNNNNNGTC 1 cut(s) 655
EarI CTCTTC 2 cut(s) 860, 997
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 4 cut(s) 495, 894, 925, 1539
Eco147I AGGCCT 1 cut(s) 1412
Eco24I GRGCYC 1 cut(s) 855
Eco31I GGTCTC 1 cut(s) 1325
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 860
EcoICRI GAGCTC 1 cut(s) 853
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 4 cut(s) 495, 894, 925, 1539
EcoT38I GRGCYC 1 cut(s) 855
ErhI CCWWGG 4 cut(s) 495, 894, 925, 1539
Esp3I CGTCTC 1 cut(s) 1466
FaeI CATG 8 cut(s) 66, 95, 275, 308, 394, 729, 1016, 1160
FalI AAGNNNNNCTT 2 cut(s) 1484, 1516
FaqI GGGAC 1 cut(s) 356
FatI CATG 8 cut(s) 62, 91, 271, 304, 390, 725, 1012, 1156
FauI CCCGC 1 cut(s) 554
FauNDI CATATG 1 cut(s) 1387
Fnu4HI GCNGC 3 cut(s) 255, 486, 842
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 3 cut(s) 255, 486, 842
FspBI CTAG 1 cut(s) 705
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
GluI GCNGC 3 cut(s) 255, 486, 842
GsaI CCCAGC 1 cut(s) 519
GsuI CTGGAG 2 cut(s) 471, 561
HaeIII GGCC 7 cut(s) 245, 336, 546, 683, 930, 1277, 1412
HapII CCGG 2 cut(s) 746, 1278
HgaI GACGC 1 cut(s) 586
HhaI GCGC 1 cut(s) 427
Hin1II CATG 8 cut(s) 66, 95, 275, 308, 394, 729, 1016, 1160
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 6 cut(s) 266, 714, 1031, 1166, 1381, 1399
HpaII CCGG 2 cut(s) 746, 1278
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 502, 995
Hpy188I TCNGA 9 cut(s) 11, 326, 367, 689, 713, 1071, 1129, 1396, 1461
Hpy188III TCNNGA 5 cut(s) 386, 450, 1189, 1433, 1451
Hpy8I GTNNAC 2 cut(s) 502, 995
Hpy99I CGWCG 3 cut(s) 367, 602, 1312
HpyAV CCTTC 5 cut(s) 628, 788, 1120, 1316, 1423
HpyCH4III ACNGT 9 cut(s) 286, 532, 631, 766, 829, 910, 1039, 1226, 1457
HpyCH4IV ACGT 5 cut(s) 118, 301, 362, 774, 1528
HpyCH4V TGCA 5 cut(s) 313, 479, 488, 833, 962
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 251, 485, 850, 973
HpyF3I CTNAG 5 cut(s) 40, 55, 593, 618, 860
HpySE526I ACGT 5 cut(s) 118, 301, 362, 774, 1528
Hsp92II CATG 8 cut(s) 66, 95, 275, 308, 394, 729, 1016, 1160
HspAI GCGC 1 cut(s) 425
Kzo9I GATC 4 cut(s) 805, 1185, 1429, 1546
LguI GCTCTTC 1 cut(s) 860
LmnI GCTCC 2 cut(s) 452, 701
Lsp1109I GCAGC 3 cut(s) 266, 472, 828
LweI GCATC 1 cut(s) 604
MaeI CTAG 1 cut(s) 705
MaeII ACGT 5 cut(s) 118, 301, 362, 774, 1528
MaeIII GTNAC 4 cut(s) 625, 760, 775, 947
MalI GATC 4 cut(s) 807, 1187, 1431, 1548
MboI GATC 4 cut(s) 805, 1185, 1429, 1546
MboII GAAGA 8 cut(s) 680, 847, 892, 1014, 1073, 1189, 1192, 1242
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 3 cut(s) 805, 1429, 1546
MhlI GDGCHC 1 cut(s) 855
MlsI TGGCCA 3 cut(s) 245, 336, 930
MluCI AATT 6 cut(s) 150, 315, 480, 986, 1510, 1555
MluNI TGGCCA 3 cut(s) 245, 336, 930
MlyI GAGTC 2 cut(s) 723, 1375
MmeI TCCRAC 3 cut(s) 345, 551, 1188
Mox20I TGGCCA 3 cut(s) 245, 336, 930
MscI TGGCCA 3 cut(s) 245, 336, 930
MseI TTAA 6 cut(s) 318, 396, 402, 417, 1355, 1503
Msp20I TGGCCA 3 cut(s) 245, 336, 930
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 746, 1278
MspR9I CCNGG 1 cut(s) 143
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 5 cut(s) 74, 251, 485, 850, 973
NdeI CATATG 1 cut(s) 1387
NdeII GATC 4 cut(s) 805, 1185, 1429, 1546
NheI GCTAGC 1 cut(s) 704
NlaIII CATG 8 cut(s) 66, 95, 275, 308, 394, 729, 1016, 1160
NlaIV GGNNCC 6 cut(s) 509, 744, 750, 1217, 1444, 1548
NmuCI GTSAC 2 cut(s) 760, 947
NsbI TGCGCA 1 cut(s) 426
NspI RCATGY 2 cut(s) 394, 1160
PceI AGGCCT 1 cut(s) 1412
PciI ACATGT 2 cut(s) 390, 1156
PciSI GCTCTTC 1 cut(s) 860
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PfeI GAWTC 4 cut(s) 266, 1031, 1166, 1399
PflMI CCANNNNNTGG 2 cut(s) 533, 937
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 3 cut(s) 256, 487, 843
PleI GAGTC 2 cut(s) 722, 1375
PpsI GAGTC 2 cut(s) 722, 1375
Ppu21I YACGTR 1 cut(s) 1529
PscI ACATGT 2 cut(s) 390, 1156
PsiI TTATAA 1 cut(s) 816
Psp124BI GAGCTC 1 cut(s) 855
Psp1406I AACGTT 2 cut(s) 118, 774
Psp6I CCWGG 1 cut(s) 141
PspFI CCCAGC 1 cut(s) 515
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 6 cut(s) 509, 744, 750, 1217, 1444, 1548
PspPI GGNCC 2 cut(s) 681, 1275
PsuI RGATCY 3 cut(s) 805, 1429, 1546
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 5 cut(s) 24, 640, 1370, 1499, 1527
RsaNI GTAC 5 cut(s) 23, 639, 1369, 1498, 1526
SacI GAGCTC 1 cut(s) 855
SapI GCTCTTC 1 cut(s) 860
SaqAI TTAA 6 cut(s) 318, 396, 402, 417, 1355, 1503
SatI GCNGC 3 cut(s) 255, 486, 842
Sau3AI GATC 4 cut(s) 805, 1185, 1429, 1546
Sau96I GGNCC 2 cut(s) 681, 1275
ScaI AGTACT 1 cut(s) 1499
SchI GAGTC 2 cut(s) 723, 1375
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 1 cut(s) 855
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 2 cut(s) 801, 1423
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 6 cut(s) 150, 315, 480, 986, 1510, 1555
SseBI AGGCCT 1 cut(s) 1412
SsiI CCGC 2 cut(s) 208, 561
SspI AATATT 2 cut(s) 886, 1201
SspMI CTAG 1 cut(s) 705
SstI GAGCTC 1 cut(s) 855
StuI AGGCCT 1 cut(s) 1412
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 4 cut(s) 495, 894, 925, 1539
TaaI ACNGT 9 cut(s) 286, 532, 631, 766, 829, 910, 1039, 1226, 1457
TaiI ACGT 5 cut(s) 121, 304, 365, 777, 1531
TaqI TCGA 4 cut(s) 345, 600, 1188, 1434
TasI AATT 6 cut(s) 150, 315, 480, 986, 1510, 1555
TatI WGTACW 2 cut(s) 22, 1497
TfiI GAWTC 4 cut(s) 266, 1031, 1166, 1399
Tru1I TTAA 6 cut(s) 318, 396, 402, 417, 1355, 1503
Tru9I TTAA 6 cut(s) 318, 396, 402, 417, 1355, 1503
TscAI CASTG 1 cut(s) 1042
TseFI GTSAC 2 cut(s) 760, 947
TseI GCWGC 3 cut(s) 254, 485, 841
Tsp45I GTSAC 2 cut(s) 760, 947
TspDTI ATGAA 8 cut(s) 17, 22, 80, 279, 714, 1029, 1250, 1335
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 1 cut(s) 1042
Van91I CCANNNNNTGG 2 cut(s) 533, 937
XceI RCATGY 2 cut(s) 394, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XspI CTAG 1 cut(s) 705
ZrmI AGTACT 1 cut(s) 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.