Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000956

Physical Location & Seq

Genomic Coordinates Forward (+)

40253269 .. 40254794

Gene Structure 1526 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1267300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGCAAACGTCTTCTGTTCAATTCTCCATCCACTCTGTCCTTTCGTGTTTACTACCATGGGCAACTTCAGATCCAGTTCTTACTGGCTTCCTCCAATGTCTTCCAAAACAATCACAGCCCACCTACCCAATCTTCGAAGCCATCTTCACCCATGACAACACCTCATTTCAGTCCGTTTTGCAACATTACATTAGAAACAGCAGATTCTTGACCCCAACAACCCCAAAACCTTTGGCTATTGTAGCAGCCAAACACGAATCACATGTGCAAGCAACCGTGATTTGTGTGAAACAACACGGGCTTCAGATTAGACTCCGTAGTGCTGGCCATGATTATGAGGGTCTATCATACGCATCTGCTGTTCCCTTTGTCATTCTTGACATGTTCAATCTTCGAGCCATTGATGTTAATCTTGCGGATGAGAGCGCTTGGGTGCAATCTGGGGCTACTCTTGGTGAACTTTATTATGAAATCGCCAATAAAAGTGAGGTTCATGGGTTCACAGCCGGAGCTTGTCTAACTGTTGGTGCCGGTGGCCACTTCAGTGGTGGCGGGTATGGAACTACGATGAGAAAATATGGGCTTACCGTGGATAATATTGAAGATGCAAAACTAGTTAATGTGAACGGAACAATTCTTGATAGAAAGTCAATGGGAGATGATCTTTTTTGGGCCATTAGAGGAGGAGGTGGAGCAAGCTTTGGAGTCATTTTGTCATGGAAAATCAAGTTGGCTCCACAAGGTGCTACAGATGTTGCTTATAAGTGGCAATATGTAGCACCGAAGCTGCCTGACAACGTCTTCATCAGAGCACAACCGATAGTGATTTCGAATAAAGGCAAAAAGACCGTGAAAGTATCATTCATTGGTCTTTTCTTGGGTCAAAGTGACAAGCTTCTTCCATTGATGAATGAAAGTTTCCCAGAACTGGGTTTACAGCAAAAAGATTGTTTTCAAATCAGTTGGATTGAATCGACTGTATTCTGGGGTAACTACCCTGTTGGAACTCCCATAAATGTTCTACTTGAACGGCCAAACGGATCGGTATTTTTCTTCAAGGTTAAGTCTGACTATGTAAAAGAGTCAATTCCAAAACAAGGATTAGAATCCTTGTGGCAGATGATGCTTAAAGTAGAGAAAGTGATGCTGCAATGGAACCCTTACGGTGGAAGAATGAGTCAGTTTTCCGAGTCAGACACTCTGTTCCCTCACAGGGCTGGAAACCTTTTTTTAATTCACTACTGGATTCATTGGTCGGAAGAAGGGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

424

Amino Acids

47.04

Weight (kDa)

7.69

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	77 - 213	3.5e-24	FAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26380 AT1G26390 AT1G26400 AT1G26410 AT1G26420 AT4G20800
fragaria_vesca	FvH4_3g11042 FvH4_3g11043 FvH4_3g11044 FvH4_3g11090
malus_domestica	MD05G1266700.v1.1 MD05G1266800.v1.1 MD05G1267200.v1.1 MD05G1267300.v1.1 MD10G1243900.v1.1 MD10G1244000.v1.1
prunus_persica	Prupe.1G032800_v2.0.a1 Prupe.4G092300_v2.0.a1 Prupe.4G098500_v2.0.a1 Prupe.4G098600_v2.0.a1 Prupe.4G098700_v2.0.a1 Prupe.4G098900_v2.0.a1 Prupe.4G099000_v2.0.a1 Prupe.4G099100_v2.0.a1 Prupe.4G099200_v2.0.a1 Prupe.4G099400_v2.0.a1 Prupe.4G230400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0017771 RchiOBHm_Chr5g0017801 RchiOBHm_Chr5g0017831 RchiOBHm_Chr5g0017861 RchiOBHm_Chr5g0017931 RchiOBHm_Chr5g0017951 RchiOBHm_Chr5g0017981
rosa_laevigata	RLG00000032314 RLG00000032315 RLG00000032316 RLG00000032324 RLG00000032325 RLG00000032328 RLG00000032349
rosa_multiflora	Rmu_sc0003375.1_g000007 Rmu_sc0003834.1_g000007 Rmu_sc0003834.1_g000013 Rmu_sc0003834.1_g000014 Rmu_sc0005929.1_g000010 Rmu_sc0005929.1_g000016 Rmu_sc0011118.1_g000003 Rmu_sc0011171.1_g000007 Rmu_sc0013814.1_g000002 Rmu_sc0014753.1_g000007
rosa_roxburghii	Rroxscaffold_1G00059450 Rroxscaffold_1G00059620 Rroxscaffold_1G00059700 Rroxscaffold_1G00059710
rosa_rugosa	Rorug05G0038600 Rorug05G0038700 Rorug05G0038900 Rorug05G0039200 Rorug05G0039300
rosa_wichuraiana	Rw5G011460 Rw5G011500 Rw5G011520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	762
AccB1I	GGYRCC	1 cut(s)	527
AciI	CCGC	2 cut(s)	416, 552
AclWI	GGATC	2 cut(s)	65, 1048
AcoI	YGGCCR	3 cut(s)	325, 535, 1031
AcuI	CTGAAG	3 cut(s)	51, 287, 526
AdeI	CACNNNGTG	1 cut(s)	743
AfeI	AGCGCT	1 cut(s)	427
AfiI	CCNNNNNNNGG	5 cut(s)	928, 929, 1097, 1166, 1213
AflIII	ACRYGT	2 cut(s)	262, 381
AgsI	TTSAA	7 cut(s)	20, 388, 602, 956, 971, 1028, 1057
AhlI	ACTAGT	1 cut(s)	613
AjuI	GAANNNNNNNTTGG	2 cut(s)	713, 745
AleI	CACNNNNGTG	1 cut(s)	543
AluBI	AGCT	4 cut(s)	512, 699, 787, 895
AluI	AGCT	4 cut(s)	512, 699, 787, 895
Alw21I	GWGCWC	1 cut(s)	814
AlwI	GGATC	2 cut(s)	65, 1048
Aor51HI	AGCGCT	1 cut(s)	427
AoxI	GGCC	4 cut(s)	325, 535, 672, 1031
ApeKI	GCWGC	3 cut(s)	245, 787, 1147
AspLEI	GCGC	1 cut(s)	428
AspS9I	GGNCC	2 cut(s)	672, 1266
AsuHPI	GGTGA	2 cut(s)	139, 467
AsuII	TTCGAA	2 cut(s)	135, 830
AvaII	GGWCC	1 cut(s)	1266
BalI	TGGCCA	2 cut(s)	327, 537
BanI	GGYRCC	1 cut(s)	527
BbsI	GAAGAC	3 cut(s)	3, 92, 793
Bbv12I	GWGCWC	1 cut(s)	814
BbvI	GCAGC	3 cut(s)	257, 774, 1134
BccI	CCATC	2 cut(s)	35, 149
BceAI	ACGGC	1 cut(s)	1046
BcuI	ACTAGT	1 cut(s)	613
BfaI	CTAG	2 cut(s)	614, 1270
BfmI	CTRYAG	1 cut(s)	747
BfoI	RGCGCY	1 cut(s)	429
BisI	GCNGC	3 cut(s)	246, 788, 1148
BlsI	GCNGC	3 cut(s)	247, 789, 1149
Bme18I	GGWCC	1 cut(s)	1266
BmgT120I	GGNCC	2 cut(s)	672, 1266
BmiI	GGNNCC	4 cut(s)	529, 735, 1157, 1267
BmrI	ACTGGG	1 cut(s)	938
BmsI	GCATC	4 cut(s)	362, 595, 1113, 1134
BmuI	ACTGGG	1 cut(s)	938
BpiI	GAAGAC	3 cut(s)	3, 92, 793
Bpu14I	TTCGAA	2 cut(s)	135, 830
BsaBI	GATNNNNATC	2 cut(s)	408, 1105
BsaJI	CCNNGG	2 cut(s)	56, 588
Bsc4I	CCNNNNNNNGG	5 cut(s)	928, 929, 1097, 1166, 1213
Bse118I	RCCGGY	1 cut(s)	530
Bse1I	ACTGG	4 cut(s)	74, 88, 933, 1247
Bse3DI	GCAATG	1 cut(s)	1157
Bse8I	GATNNNNATC	2 cut(s)	408, 1105
BseDI	CCNNGG	2 cut(s)	56, 588
BseGI	GGATG	2 cut(s)	27, 424
BseJI	GATNNNNATC	2 cut(s)	408, 1105
BseLI	CCNNNNNNNGG	5 cut(s)	928, 929, 1097, 1166, 1213
BseMI	GCAATG	1 cut(s)	1157
BseNI	ACTGG	4 cut(s)	74, 88, 933, 1247
BseRI	GAGGAG	2 cut(s)	696, 699
BseXI	GCAGC	3 cut(s)	257, 774, 1134
BshFI	GGCC	4 cut(s)	327, 537, 674, 1033
BshNI	GGYRCC	1 cut(s)	527
BsiHKAI	GWGCWC	1 cut(s)	814
BsiSI	CCGG	2 cut(s)	507, 531
BslI	CCNNNNNNNGG	5 cut(s)	928, 929, 1097, 1166, 1213
BsnI	GGCC	4 cut(s)	327, 537, 674, 1033
Bsp119I	TTCGAA	2 cut(s)	135, 830
Bsp1286I	GDGCHC	1 cut(s)	814
Bsp143I	GATC	3 cut(s)	70, 661, 1040
Bsp19I	CCATGG	1 cut(s)	56
BspACI	CCGC	2 cut(s)	416, 552
BspANI	GGCC	4 cut(s)	327, 537, 674, 1033
BspLI	GGNNCC	4 cut(s)	529, 735, 1157, 1267
BspPI	GGATC	2 cut(s)	65, 1048
BspT104I	TTCGAA	2 cut(s)	135, 830
BspT107I	GGYRCC	1 cut(s)	527
BsrDI	GCAATG	1 cut(s)	1157
BsrFI	RCCGGY	1 cut(s)	530
BsrI	ACTGG	4 cut(s)	74, 88, 933, 1247
BssAI	RCCGGY	1 cut(s)	530
BssECI	CCNNGG	2 cut(s)	56, 588
BssMI	GATC	3 cut(s)	70, 661, 1040
BssT1I	CCWWGG	1 cut(s)	56
Bst4CI	ACNGT	6 cut(s)	277, 523, 589, 850, 979, 1166
BstAPI	GCANNNNNTGC	1 cut(s)	1123
BstBI	TTCGAA	2 cut(s)	135, 830
BstC8I	GCNNGC	3 cut(s)	270, 325, 697
BstDSI	CCRYGG	2 cut(s)	56, 588
BstF5I	GGATG	2 cut(s)	27, 424
BstH2I	RGCGCY	1 cut(s)	429
BstHHI	GCGC	1 cut(s)	428
BstKTI	GATC	3 cut(s)	73, 664, 1043
BstMBI	GATC	3 cut(s)	70, 661, 1040
BstMWI	GCNNNNNNNGC	2 cut(s)	242, 1123
BstNSI	RCATGY	2 cut(s)	266, 385
BstSFI	CTRYAG	1 cut(s)	747
BstV1I	GCAGC	3 cut(s)	257, 774, 1134
BstV2I	GAAGAC	3 cut(s)	3, 92, 793
BstX2I	RGATCY	1 cut(s)	70
BstXI	CCANNNNNNTGG	1 cut(s)	545
BstYI	RGATCY	1 cut(s)	70
BsuRI	GGCC	4 cut(s)	327, 537, 674, 1033
BtgI	CCRYGG	2 cut(s)	56, 588
BtsCI	GGATG	2 cut(s)	27, 424
BtsIMutI	CAGTG	1 cut(s)	550
Cac8I	GCNNGC	3 cut(s)	270, 325, 697
CfoI	GCGC	1 cut(s)	428
Cfr10I	RCCGGY	1 cut(s)	530
Cfr13I	GGNCC	2 cut(s)	672, 1266
CviAII	CATG	7 cut(s)	57, 152, 263, 329, 382, 494, 717
DpnI	GATC	3 cut(s)	72, 663, 1042
DpnII	GATC	3 cut(s)	70, 661, 1040
DraIII	CACNNNGTG	1 cut(s)	743
EaeI	YGGCCR	3 cut(s)	325, 535, 1031
Eco130I	CCWWGG	1 cut(s)	56
Eco47I	GGWCC	1 cut(s)	1266
Eco47III	AGCGCT	1 cut(s)	427
Eco57I	CTGAAG	3 cut(s)	51, 287, 526
EcoO109I	RGGNCCY	1 cut(s)	1266
EcoT14I	CCWWGG	1 cut(s)	56
ErhI	CCWWGG	1 cut(s)	56
FaeI	CATG	7 cut(s)	60, 155, 266, 332, 385, 497, 720
FatI	CATG	7 cut(s)	56, 151, 262, 328, 381, 493, 716
FauI	CCCGC	1 cut(s)	545
Fnu4HI	GCNGC	3 cut(s)	246, 788, 1148
FokI	GGATG	2 cut(s)	14, 431
Fsp4HI	GCNGC	3 cut(s)	246, 788, 1148
FspBI	CTAG	2 cut(s)	614, 1270
GlaI	GCGC	1 cut(s)	427
GluI	GCNGC	3 cut(s)	246, 788, 1148
HaeII	RGCGCY	1 cut(s)	429
HaeIII	GGCC	4 cut(s)	327, 537, 674, 1033
HapII	CCGG	2 cut(s)	507, 531
HhaI	GCGC	1 cut(s)	428
Hin1II	CATG	7 cut(s)	60, 155, 266, 332, 385, 497, 720
Hin6I	GCGC	1 cut(s)	426
HinP1I	GCGC	1 cut(s)	426
HindIII	AAGCTT	2 cut(s)	697, 893
HpaII	CCGG	2 cut(s)	507, 531
HphI	GGTGA	2 cut(s)	139, 467
Hpy166II	GTNNAC	5 cut(s)	50, 458, 501, 625, 935
Hpy188I	TCNGA	7 cut(s)	70, 306, 809, 1069, 1189, 1195, 1258
Hpy188III	TCNNGA	3 cut(s)	208, 377, 638
Hpy8I	GTNNAC	5 cut(s)	50, 458, 501, 625, 935
HpyAV	CCTTC	1 cut(s)	1256
HpyCH4III	ACNGT	6 cut(s)	277, 523, 589, 850, 979, 1166
HpyCH4IV	ACGT	2 cut(s)	8, 798
HpyCH4V	TGCA	6 cut(s)	4, 181, 268, 436, 608, 1150
HpyF10VI	GCNNNNNNNGC	2 cut(s)	242, 1123
HpySE526I	ACGT	2 cut(s)	8, 798
Hsp92II	CATG	7 cut(s)	60, 155, 266, 332, 385, 497, 720
HspAI	GCGC	1 cut(s)	426
Kzo9I	GATC	3 cut(s)	70, 661, 1040
LmnI	GCTCC	3 cut(s)	509, 692, 739
Lsp1109I	GCAGC	3 cut(s)	257, 774, 1134
LweI	GCATC	4 cut(s)	362, 595, 1113, 1134
MaeI	CTAG	2 cut(s)	614, 1270
MaeII	ACGT	2 cut(s)	8, 798
MaeIII	GTNAC	2 cut(s)	887, 989
MalI	GATC	3 cut(s)	72, 663, 1042
MboI	GATC	3 cut(s)	70, 661, 1040
MflI	RGATCY	1 cut(s)	70
MhlI	GDGCHC	1 cut(s)	814
MlsI	TGGCCA	2 cut(s)	327, 537
MluCI	AATT	4 cut(s)	20, 633, 1086, 1233
MluNI	TGGCCA	2 cut(s)	327, 537
MlyI	GAGTC	5 cut(s)	306, 714, 1091, 1186, 1199
MmeI	TCCRAC	3 cut(s)	944, 982, 1236
MnlI	CCTC	8 cut(s)	101, 172, 331, 481, 674, 677, 680, 1218
Mox20I	TGGCCA	2 cut(s)	327, 537
MscI	TGGCCA	2 cut(s)	327, 537
MseI	TTAA	5 cut(s)	408, 618, 1062, 1128, 1232
MslI	CAYNNNNRTG	2 cut(s)	333, 543
Msp20I	TGGCCA	2 cut(s)	327, 537
MspI	CCGG	2 cut(s)	507, 531
MwoI	GCNNNNNNNGC	2 cut(s)	242, 1123
NcoI	CCATGG	1 cut(s)	56
NdeII	GATC	3 cut(s)	70, 661, 1040
NlaIII	CATG	7 cut(s)	60, 155, 266, 332, 385, 497, 720
NlaIV	GGNNCC	4 cut(s)	529, 735, 1157, 1267
NmuCI	GTSAC	1 cut(s)	887
NspI	RCATGY	2 cut(s)	266, 385
NspV	TTCGAA	2 cut(s)	135, 830
OliI	CACNNNNGTG	1 cut(s)	543
PciI	ACATGT	2 cut(s)	262, 381
PfeI	GAWTC	5 cut(s)	204, 257, 971, 1106, 1246
PflFI	GACNNNGTC	1 cut(s)	797
PkrI	GCNGC	3 cut(s)	247, 789, 1149
PleI	GAGTC	5 cut(s)	306, 713, 1090, 1185, 1198
PpsI	GAGTC	5 cut(s)	306, 713, 1090, 1185, 1198
PpuMI	RGGWCCY	1 cut(s)	1266
PscI	ACATGT	2 cut(s)	262, 381
PsiI	TTATAA	1 cut(s)	762
Psp5II	RGGWCCY	1 cut(s)	1266
PspN4I	GGNNCC	4 cut(s)	529, 735, 1157, 1267
PspPI	GGNCC	2 cut(s)	672, 1266
PspPPI	RGGWCCY	1 cut(s)	1266
PsrI	GAACNNNNNNTAC	2 cut(s)	918, 950
PsuI	RGATCY	1 cut(s)	70
PsyI	GACNNNGTC	1 cut(s)	797
RseI	CAYNNNNRTG	2 cut(s)	333, 543
SaqAI	TTAA	5 cut(s)	408, 618, 1062, 1128, 1232
SatI	GCNGC	3 cut(s)	246, 788, 1148
Sau3AI	GATC	3 cut(s)	70, 661, 1040
Sau96I	GGNCC	2 cut(s)	672, 1266
SchI	GAGTC	5 cut(s)	306, 714, 1091, 1186, 1199
SduI	GDGCHC	1 cut(s)	814
SfaNI	GCATC	4 cut(s)	362, 595, 1113, 1134
SfcI	CTRYAG	1 cut(s)	747
SfuI	TTCGAA	2 cut(s)	135, 830
SinI	GGWCC	1 cut(s)	1266
SmiMI	CAYNNNNRTG	2 cut(s)	333, 543
SpeI	ACTAGT	1 cut(s)	613
Sse9I	AATT	4 cut(s)	20, 633, 1086, 1233
SsiI	CCGC	2 cut(s)	416, 552
SspI	AATATT	1 cut(s)	598
SspMI	CTAG	2 cut(s)	614, 1270
StyI	CCWWGG	1 cut(s)	56
TaaI	ACNGT	6 cut(s)	277, 523, 589, 850, 979, 1166
TaiI	ACGT	2 cut(s)	11, 801
TaqI	TCGA	4 cut(s)	135, 394, 830, 974
TasI	AATT	4 cut(s)	20, 633, 1086, 1233
TfiI	GAWTC	5 cut(s)	204, 257, 971, 1106, 1246
Tru1I	TTAA	5 cut(s)	408, 618, 1062, 1128, 1232
Tru9I	TTAA	5 cut(s)	408, 618, 1062, 1128, 1232
TscAI	CASTG	1 cut(s)	550
TseFI	GTSAC	1 cut(s)	887
TseI	GCWGC	3 cut(s)	245, 787, 1147
Tsp45I	GTSAC	1 cut(s)	887
TspDTI	ATGAA	7 cut(s)	482, 483, 793, 853, 923, 927, 1238
TspGWI	ACGGA	4 cut(s)	163, 305, 642, 1053
TspRI	CASTG	1 cut(s)	550
Tth111I	GACNNNGTC	1 cut(s)	797
VpaK11BI	GGWCC	1 cut(s)	1266
XceI	RCATGY	2 cut(s)	266, 385
XcmI	CCANNNNNNNNNTGG	1 cut(s)	545
XspI	CTAG	2 cut(s)	614, 1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.