Prupe.4G099200_v2.0.a1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000956
Physical Location & Seq
Forward (+)
5051036 .. 5054467
3432 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G099200.1

Sequence Viewer

Length: 1524 bp
ATGGTGGTGGAGATCCGACACCGAAATGCAGGATCATGGGCAACTTCAGAACCCGTTCCCATTAATGGGTTTCTCCAATGCCTTCCAAAACAGTTCATTTCTGGCCGCCCAACCTTGAAACCGTTTACCCCCCTTCAAAACACCTCTTTTCGCCCTACAACTCCAAAACCTCTGGCCATTGTAGCAGCCAAGCACGAATCTCATGTGCAGGCAACTATTGTTTGTGCAAAGCAACATGGATTGCAAATTAGAATCCGAAGTGGTGGCCATGACTATGAGGGTCTATCGTACGTATCGCCTGTTCCCTTTGTCGTTCTGGACATGTTTCATTTCCAATCCGTTGATGTTAATGTCAAGGATGAGAGTGCTTGGGTTGAGTCTGGAGCTACTCTTGGCAAAATTTATTATAAAATTGCTGAGAAAACCAAGGTTCATGGGTTTCCTGCTGGGGTTTGTCCAACTGTTGGTGCTGGTGGCCACATTAGTGGTGGAGGGTATGGGAATTTGATGAGAAAATACGGCCTTAGCATCGACAATATCGTCGATGCTAAACTAGTTGATGCAAATGGTAGAATCCTTGATAGAAAGTCTATGGGAGAAGACATTTTCTGGGCCCTTAGAGGAGGTGGAGGAGCTAGCTTTGGAGTCATTCTTTCATGGAAACTGAAGCTTGTTCGAGTTCCAGAAAAAGTGACCGTGTTCAACGTTACAAAGACATTAGAGCAAGGCGTTACAGATGTCCTTTATAAGTGGCAATTTGTAGCACCCGTGCTCCCTAAAGACCTCTTCCTGAGAGCAAGGCCAGAAATCGAGAATAATACTGAGGGCAAGAAGACTGCGGAAGCATCATTCATTGGTCATTTTTTGGGGCAAACCGACAAGCTTCTTCCATTGATGAACGAGAGATTCCCTGAATTGGGTTTACGACGAGAAGATTGTCATGAAGTGAGTTGGATTGAATCCGTTGTATTCTGGGATGAATTCCCACTTGGAACCCCAATAACTATTCTTCTCAACCAGACAGCCACACCAATACCCTTCTTCAAAGCTAAGTCTGACTATGTGAAGAAACCAATTCCAAAAAAAGTTATAGAATACATGTTTAAGGCGATGCTTGAGATAGGGAATATATGGACGGAATGGAACCCTCATGGTGGAAGAATGAGTGAGATTTCTGCGTCGGCAACTCCATACCCTCACAGAGCTGGAAACCTGTATATGATTCAATACTATACGTCGTGGTATCAAGACGAAGGAATTGAGGCCACCAACAGGTACATTCACTTAACAAGAAAGTTGTATGAAAAGATGGCTCCGTTTGTCTCCAGGAACCCGAGAGAGGCCTTCCAAAACTATAGGGATCTCGACATTGGCGCCAATCTGTACAACCAAAGTAACCTTCTAACTGCTAGAGTTTACGGAAGCAAGTATTTTAAAGGTAACTTTGAAAGATTGGTGCGTGTGAAAACCGTGGTTGATCCTCACAACTTTTTCAAGCATGAACAAAGTATCCCACCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

508

Amino Acids

57.25

Weight (kDa)

9.26

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 408, 747
AasI GACNNNNNNGTC 1 cut(s) 539
AccB1I GGYRCC 1 cut(s) 1373
AccB7I CCANNNNNTGG 1 cut(s) 464
AciI CCGC 2 cut(s) 106, 839
AclI AACGTT 1 cut(s) 705
AclWI GGATC 4 cut(s) 7, 40, 1368, 1472
AcoI YGGCCR 4 cut(s) 103, 174, 265, 475
AcsI RAATTY 3 cut(s) 399, 502, 980
AcuI CTGAAG 2 cut(s) 30, 686
AcyI GRCGYC 1 cut(s) 1374
AfaI GTAC 3 cut(s) 290, 1277, 1385
AfiI CCNNNNNNNGG 8 cut(s) 65, 66, 464, 916, 917, 1154, 1272, 1339
AflIII ACRYGT 2 cut(s) 321, 1098
AgsI TTSAA 8 cut(s) 118, 137, 703, 959, 1045, 1226, 1448, 1495
AhlI ACTAGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 1325
AjuI GAANNNNNNNTTGG 4 cut(s) 972, 1004, 1383, 1415
AleI CACNNNNGTG 1 cut(s) 483
AloI GAACNNNNNNTCC 1 cut(s) 1494
AluBI AGCT 7 cut(s) 386, 635, 639, 670, 883, 1049, 1205
AluI AGCT 7 cut(s) 386, 635, 639, 670, 883, 1049, 1205
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 1 cut(s) 1327
AlwI GGATC 4 cut(s) 7, 40, 1368, 1472
Ama87I CYCGRG 1 cut(s) 1333
AoxI GGCC 9 cut(s) 103, 174, 265, 475, 520, 612, 800, 1263, 1341
ApaI GGGCCC 1 cut(s) 616
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 3 cut(s) 399, 502, 980
AseI ATTAAT 1 cut(s) 63
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 1 cut(s) 1376
AspS9I GGNCC 2 cut(s) 612, 613
AsuNHI GCTAGC 1 cut(s) 635
AvaI CYCGRG 1 cut(s) 1333
BaeGI GKGCMC 1 cut(s) 616
BalI TGGCCA 3 cut(s) 176, 267, 477
BanI GGYRCC 1 cut(s) 1373
BanII GRGCYC 1 cut(s) 616
BbsI GAAGAC 2 cut(s) 606, 839
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 1 cut(s) 197
BccI CCATC 1 cut(s) 1303
BceAI ACGGC 1 cut(s) 535
BciT130I CCWGG 1 cut(s) 1327
BciVI GTATCC 1 cut(s) 1520
BcoDI GTCTC 1 cut(s) 1327
BcuI ACTAGT 1 cut(s) 553
BfaI CTAG 3 cut(s) 554, 636, 1410
BfmI CTRYAG 1 cut(s) 1354
BfoI RGCGCY 1 cut(s) 1377
BfuI GTATCC 1 cut(s) 1520
BisI GCNGC 2 cut(s) 106, 186
BlsI GCNGC 2 cut(s) 107, 187
Bme1390I CCNGG 1 cut(s) 1327
BmeT110I CYCGRG 1 cut(s) 1333
BmgT120I GGNCC 2 cut(s) 612, 613
BmiI GGNNCC 6 cut(s) 614, 994, 1145, 1314, 1331, 1375
BmrFI CCNGG 1 cut(s) 1327
BmsI GCATC 5 cut(s) 535, 537, 550, 854, 1101
BmtI GCTAGC 1 cut(s) 639
BpiI GAAGAC 2 cut(s) 606, 839
BpmI CTGGAG 2 cut(s) 402, 1309
Bpu10I CCTNAGC 1 cut(s) 524
BpuEI CTTGAG 1 cut(s) 1136
BsaAI YACGTR 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 1374
BsaJI CCNNGG 2 cut(s) 426, 1470
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 8 cut(s) 65, 66, 464, 916, 917, 1154, 1272, 1339
BseBI CCWGG 1 cut(s) 1327
BseDI CCNNGG 2 cut(s) 426, 1470
BseGI GGATG 2 cut(s) 364, 982
BseLI CCNNNNNNNGG 8 cut(s) 65, 66, 464, 916, 917, 1154, 1272, 1339
BseMII CTCAG 3 cut(s) 408, 782, 813
BseRI GAGGAG 2 cut(s) 636, 645
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 1 cut(s) 197
BseYI CCCAGC 1 cut(s) 446
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 9 cut(s) 105, 176, 267, 477, 522, 614, 802, 1265, 1343
BshNI GGYRCC 1 cut(s) 1373
BsiHKAI GWGCWC 1 cut(s) 774
BsiHKCI CYCGRG 1 cut(s) 1333
BsiWI CGTACG 1 cut(s) 288
BslI CCNNNNNNNGG 8 cut(s) 65, 66, 464, 916, 917, 1154, 1272, 1339
BsmAI GTCTC 1 cut(s) 1327
BsnI GGCC 9 cut(s) 105, 176, 267, 477, 522, 614, 802, 1265, 1343
BsoBI CYCGRG 1 cut(s) 1333
Bsp120I GGGCCC 1 cut(s) 612
Bsp1286I GDGCHC 2 cut(s) 616, 774
Bsp1407I TGTACA 1 cut(s) 1383
Bsp143I GATC 4 cut(s) 12, 32, 1360, 1477
BspACI CCGC 2 cut(s) 106, 839
BspANI GGCC 9 cut(s) 105, 176, 267, 477, 522, 614, 802, 1265, 1343
BspCNI CTCAG 3 cut(s) 409, 783, 814
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 6 cut(s) 614, 994, 1145, 1314, 1331, 1375
BspOI GCTAGC 1 cut(s) 639
BspPI GGATC 4 cut(s) 7, 40, 1368, 1472
BspT107I GGYRCC 1 cut(s) 1373
BsrGI TGTACA 1 cut(s) 1383
BssECI CCNNGG 2 cut(s) 426, 1470
BssMI GATC 4 cut(s) 12, 32, 1360, 1477
BssNI GRCGYC 1 cut(s) 1374
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 1327
Bst4CI ACNGT 5 cut(s) 93, 123, 463, 697, 1471
Bst6I CTCTTC 1 cut(s) 791
BstACI GRCGYC 1 cut(s) 1374
BstAUI TGTACA 1 cut(s) 1383
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 210, 637
BstDEI CTNAG 6 cut(s) 417, 524, 617, 791, 822, 1050
BstDSI CCRYGG 1 cut(s) 1470
BstF5I GGATG 2 cut(s) 364, 982
BstH2I RGCGCY 1 cut(s) 1377
BstHHI GCGC 1 cut(s) 1376
BstKTI GATC 4 cut(s) 15, 35, 1363, 1480
BstMAI GTCTC 1 cut(s) 1327
BstMBI GATC 4 cut(s) 12, 32, 1360, 1477
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 1327
BstNSI RCATGY 2 cut(s) 325, 1102
BstSCI CCNGG 1 cut(s) 1325
BstSFI CTRYAG 1 cut(s) 1354
BstSLI GKGCMC 1 cut(s) 616
BstSNI TACGTA 1 cut(s) 292
BstV1I GCAGC 1 cut(s) 197
BstV2I GAAGAC 2 cut(s) 606, 839
BstX2I RGATCY 2 cut(s) 12, 1360
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 2 cut(s) 12, 1360
BsuI GTATCC 1 cut(s) 1520
BsuRI GGCC 9 cut(s) 105, 176, 267, 477, 522, 614, 802, 1265, 1343
BtgI CCRYGG 1 cut(s) 1470
BtgZI GCGATG 1 cut(s) 1124
BtsCI GGATG 2 cut(s) 364, 982
Cac8I GCNNGC 2 cut(s) 210, 637
CciI TCATGA 1 cut(s) 940
CfoI GCGC 1 cut(s) 1376
Cfr13I GGNCC 2 cut(s) 612, 613
CseI GACGC 1 cut(s) 1167
Csp6I GTAC 3 cut(s) 289, 1276, 1384
CviQI GTAC 3 cut(s) 289, 1276, 1384
DdeI CTNAG 6 cut(s) 417, 524, 617, 791, 822, 1050
DinI GGCGCC 1 cut(s) 1375
DpnI GATC 4 cut(s) 14, 34, 1362, 1479
DpnII GATC 4 cut(s) 12, 32, 1360, 1477
DraI TTTAAA 1 cut(s) 1435
DrdI GACNNNNNNGTC 1 cut(s) 539
DseDI GACNNNNNNGTC 1 cut(s) 539
EaeI YGGCCR 4 cut(s) 103, 174, 265, 475
Eam1104I CTCTTC 1 cut(s) 791
EarI CTCTTC 1 cut(s) 791
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 1343
Eco24I GRGCYC 1 cut(s) 616
Eco57I CTGAAG 2 cut(s) 30, 686
Eco88I CYCGRG 1 cut(s) 1333
EcoO109I RGGNCCY 1 cut(s) 613
EcoRI GAATTC 1 cut(s) 980
EcoRII CCWGG 1 cut(s) 1325
EcoT14I CCWWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 616
EgeI GGCGCC 1 cut(s) 1375
EheI GGCGCC 1 cut(s) 1375
ErhI CCWWGG 1 cut(s) 426
Fnu4HI GCNGC 2 cut(s) 106, 186
FokI GGATG 2 cut(s) 371, 989
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 2 cut(s) 106, 186
FspBI CTAG 3 cut(s) 554, 636, 1410
GlaI GCGC 1 cut(s) 1375
GluI GCNGC 2 cut(s) 106, 186
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 2 cut(s) 402, 1309
HaeII RGCGCY 1 cut(s) 1377
HaeIII GGCC 9 cut(s) 105, 176, 267, 477, 522, 614, 802, 1265, 1343
HgaI GACGC 1 cut(s) 1167
HhaI GCGC 1 cut(s) 1376
Hin1I GRCGYC 1 cut(s) 1374
Hin6I GCGC 1 cut(s) 1374
HinP1I GCGC 1 cut(s) 1374
HindIII AAGCTT 2 cut(s) 668, 881
HinfI GANTC 8 cut(s) 197, 252, 377, 573, 645, 906, 959, 1222
Hpy166II GTNNAC 3 cut(s) 126, 923, 1417
Hpy188I TCNGA 4 cut(s) 17, 49, 257, 1057
Hpy188III TCNNGA 8 cut(s) 317, 381, 683, 790, 811, 941, 1247, 1364
Hpy8I GTNNAC 3 cut(s) 126, 923, 1417
Hpy99I CGWCG 4 cut(s) 545, 930, 1183, 1240
HpyAV CCTTC 6 cut(s) 92, 143, 1048, 1247, 1354, 1409
HpyCH4III ACNGT 5 cut(s) 93, 123, 463, 697, 1471
HpyCH4IV ACGT 3 cut(s) 291, 705, 1235
HpyCH4V TGCA 5 cut(s) 29, 208, 227, 244, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 6 cut(s) 417, 524, 617, 791, 822, 1050
HpySE526I ACGT 3 cut(s) 291, 705, 1235
Hsp92I GRCGYC 1 cut(s) 1374
HspAI GCGC 1 cut(s) 1374
KasI GGCGCC 1 cut(s) 1373
Kzo9I GATC 4 cut(s) 12, 32, 1360, 1477
LmnI GCTCC 4 cut(s) 383, 632, 777, 1318
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 5 cut(s) 535, 537, 550, 854, 1101
MaeI CTAG 3 cut(s) 554, 636, 1410
MaeII ACGT 3 cut(s) 291, 705, 1235
MaeIII GTNAC 5 cut(s) 691, 706, 730, 1394, 1439
MalI GATC 4 cut(s) 14, 34, 1362, 1479
MboI GATC 4 cut(s) 12, 32, 1360, 1477
MboII GAAGA 9 cut(s) 611, 778, 844, 878, 944, 1001, 1033, 1078, 1170
MflI RGATCY 2 cut(s) 12, 1360
MhlI GDGCHC 2 cut(s) 616, 774
MlsI TGGCCA 3 cut(s) 176, 267, 477
MluCI AATT 9 cut(s) 246, 399, 411, 502, 755, 914, 980, 1074, 1257
MluNI TGGCCA 3 cut(s) 176, 267, 477
Mly113I GGCGCC 1 cut(s) 1374
MlyI GAGTC 2 cut(s) 386, 654
MmeI TCCRAC 3 cut(s) 40, 482, 932
Mox20I TGGCCA 3 cut(s) 176, 267, 477
MroXI GAANNNNTTC 1 cut(s) 54
MscI TGGCCA 3 cut(s) 176, 267, 477
MseI TTAA 5 cut(s) 63, 348, 1104, 1286, 1434
MslI CAYNNNNRTG 3 cut(s) 24, 273, 483
Msp20I TGGCCA 3 cut(s) 176, 267, 477
MspR9I CCNGG 1 cut(s) 1327
MvaI CCWGG 1 cut(s) 1327
MwoI GCNNNNNNNGC 1 cut(s) 182
NarI GGCGCC 1 cut(s) 1374
NdeII GATC 4 cut(s) 12, 32, 1360, 1477
NheI GCTAGC 1 cut(s) 635
NlaIV GGNNCC 6 cut(s) 614, 994, 1145, 1314, 1331, 1375
NmuCI GTSAC 1 cut(s) 691
NspI RCATGY 2 cut(s) 325, 1102
OliI CACNNNNGTG 1 cut(s) 483
PagI TCATGA 1 cut(s) 940
PceI AGGCCT 1 cut(s) 1343
PciI ACATGT 2 cut(s) 321, 1098
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 6 cut(s) 197, 252, 573, 906, 959, 1222
Pfl23II CGTACG 1 cut(s) 288
PflMI CCANNNNNTGG 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 1325
PkrI GCNGC 2 cut(s) 107, 187
PleI GAGTC 2 cut(s) 385, 653
PluTI GGCGCC 1 cut(s) 1377
PpsI GAGTC 2 cut(s) 385, 653
Ppu21I YACGTR 1 cut(s) 292
PscI ACATGT 2 cut(s) 321, 1098
PshBI ATTAAT 1 cut(s) 63
PsiI TTATAA 2 cut(s) 408, 747
Psp1406I AACGTT 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 1325
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 1 cut(s) 1325
PspLI CGTACG 1 cut(s) 288
PspN4I GGNNCC 6 cut(s) 614, 994, 1145, 1314, 1331, 1375
PspOMI GGGCCC 1 cut(s) 612
PspPI GGNCC 2 cut(s) 612, 613
PsrI GAACNNNNNNTAC 2 cut(s) 285, 317
PsuI RGATCY 2 cut(s) 12, 1360
RsaI GTAC 3 cut(s) 290, 1277, 1385
RsaNI GTAC 3 cut(s) 289, 1276, 1384
RseI CAYNNNNRTG 3 cut(s) 24, 273, 483
SaqAI TTAA 5 cut(s) 63, 348, 1104, 1286, 1434
SatI GCNGC 2 cut(s) 106, 186
Sau3AI GATC 4 cut(s) 12, 32, 1360, 1477
Sau96I GGNCC 2 cut(s) 612, 613
SchI GAGTC 2 cut(s) 386, 654
ScrFI CCNGG 1 cut(s) 1327
SduI GDGCHC 2 cut(s) 616, 774
SfaNI GCATC 5 cut(s) 535, 537, 550, 854, 1101
SfcI CTRYAG 1 cut(s) 1354
SfoI GGCGCC 1 cut(s) 1375
SmiMI CAYNNNNRTG 3 cut(s) 24, 273, 483
SmlI CTYRAG 1 cut(s) 1115
SmoI CTYRAG 1 cut(s) 1115
SnaBI TACGTA 1 cut(s) 292
SpeI ACTAGT 1 cut(s) 553
Sse9I AATT 9 cut(s) 246, 399, 411, 502, 755, 914, 980, 1074, 1257
SseBI AGGCCT 1 cut(s) 1343
SsiI CCGC 2 cut(s) 106, 839
SspDI GGCGCC 1 cut(s) 1373
SspMI CTAG 3 cut(s) 554, 636, 1410
StuI AGGCCT 1 cut(s) 1343
StyD4I CCNGG 1 cut(s) 1325
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 5 cut(s) 93, 123, 463, 697, 1471
TaiI ACGT 3 cut(s) 294, 708, 1238
TaqI TCGA 5 cut(s) 531, 543, 676, 810, 1365
TasI AATT 9 cut(s) 246, 399, 411, 502, 755, 914, 980, 1074, 1257
TatI WGTACW 1 cut(s) 1383
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 6 cut(s) 197, 252, 573, 906, 959, 1222
Tru1I TTAA 5 cut(s) 63, 348, 1104, 1286, 1434
Tru9I TTAA 5 cut(s) 63, 348, 1104, 1286, 1434
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 691
TspGWI ACGGA 5 cut(s) 328, 952, 1151, 1305, 1434
Van91I CCANNNNNTGG 1 cut(s) 464
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 3 cut(s) 399, 502, 980
XceI RCATGY 2 cut(s) 325, 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 3 cut(s) 554, 636, 1410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.