Rroxscaffold_1G00059710

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000956
Physical Location & Seq
Forward (+)
81784044 .. 81785448
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059710.1

Sequence Viewer

Length: 1296 bp
ATGAGAATCCGAAGTGGTGGCCACGACTACGAGGGCTTATCCTTCATATCATACATTTCAAATACCTCATATATAGTCCTTGACATGTTTTACCTTCGAGAGATTAATGTTAACATCGCAGATGAAAGTGCTTGGGTTGGATCTGGAGCTATTGTTGGAGAACTATACTATAGCATTGCAGAAAAAAGCAAAGTTCATGCATTTCCCGGGGGAGTTTGCCCAAGTGTTGGTGTCGGTGGTCTTTTTAGTGGTGGTGGGTACGGTCCTACTATGAGAAAATTTGGGCTTACAGTGGACAACATAGTAGATGCTAGACTAGTCAATGTGAATGGTACAATCCTTGATAGAAAATTGATGGGAGAAGACCTATTTTGGGCCATTAGAGGAGGTGGTGGAGCAAGCTTTGGAGTCATTCTTTCATGGAAGATCAAATTGCTTCAAGTTCCACCCATTGTCACTGTTTTCAGAATTACAAAGACACTGGCGCAAGGCGCTATGGATGTTCTTCACAGGTGGCAATACGTTGCGCCTAACCTACCTAAAGAACTCTTCATTAGAGCAACTTCAAAAGTCCAGAATAGCACTACCGAAGCAGGAAAGAAGACTGTGGAGGTGTTATTCAATGGTCTCTATCTAGGACAACGGGAAAACCTTCTTCCTTTGGTAAGTGACAGTTTCCCTGAATTGGGTTTGGAGCAAAAAGATTGCTTCGAAATGAGTTGGATTGAATCCACTTTATTCTTTTCCGAATACGCATTTGGAACTCCCATAAAGGCTCTAGTAAAAAGACCAACGGAACCACCTCCATTCTTCTTCAAAGGCAAGTCGGATTATGTAAAACAACCAATTCCGAAAGAGGTCTTGAAGTCGATATTCGAGAAGATGATCAATCGATACGAGTCGACGCACATGCAATGGAACCCATATGGTGGAAGAATGAATGAGATTTCCGAGTCGGAAACTCCATTCCCTCACAGAGCAGGAAACCTGTTTATGATTCAGTACTGGACGTATTGGATGGAAAAAGGAATTGAGAGCACCAACAAGTACCTTAACTTGTCGAGAAATTTGTACGGAAGCATGACTCCGTACGTGTCCAAGAGCCCAAGAGAGGTCTTGCATAACTATAGGGATCTTGACATAGGCTCCAATATGCTTAACCAGACCAGCATTGAGCATGCTCGAGTTTATGGAAGAACGTATTTTAAAGGCAATTTTGAAAGATTGGTACGTGTGAAAAGTGCTGTTGATCCTCAGAATTTTTTCAAGCATGAACAAAGTATCCCACCGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

431

Amino Acids

48.82

Weight (kDa)

8.97

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 2 - 114 6.6e-16 FAD binding domain
BBE PF08031 375 - 429 1.2e-17 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 227, 929
AccI GTMKAC 1 cut(s) 902
AclWI GGATC 3 cut(s) 148, 1140, 1244
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 3 cut(s) 278, 1066, 1258
AfaI GTAC 7 cut(s) 260, 334, 1004, 1049, 1073, 1091, 1230
AfiI CCNNNNNNNGG 6 cut(s) 227, 373, 685, 686, 929, 1111
AflIII ACRYGT 3 cut(s) 84, 1092, 1231
AgsI TTSAA 9 cut(s) 60, 440, 567, 622, 728, 817, 865, 1220, 1267
AhlI ACTAGT 1 cut(s) 316
AjuI GAANNNNNNNTTGG 2 cut(s) 741, 773
AloI GAACNNNNNNTCC 1 cut(s) 1266
AluBI AGCT 2 cut(s) 149, 402
AluI AGCT 2 cut(s) 149, 402
Alw21I GWGCWC 1 cut(s) 1040
Alw26I GTCTC 1 cut(s) 632
AlwI GGATC 3 cut(s) 148, 1140, 1244
Ama87I CYCGRG 2 cut(s) 206, 1182
AoxI GGCC 2 cut(s) 19, 375
ApoI RAATTY 3 cut(s) 278, 1066, 1258
AseI ATTAAT 1 cut(s) 105
Asp700I GAANNNNTTC 2 cut(s) 651, 1262
AspLEI GCGC 3 cut(s) 487, 494, 529
AspS9I GGNCC 2 cut(s) 263, 375
AsuC2I CCSGG 2 cut(s) 207, 208
AsuII TTCGAA 1 cut(s) 711
AvaI CYCGRG 2 cut(s) 206, 1182
AvaII GGWCC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 21
BanII GRGCYC 1 cut(s) 1106
BbsI GAAGAC 2 cut(s) 369, 608
Bbv12I GWGCWC 1 cut(s) 1040
BccI CCATC 2 cut(s) 349, 1012
BcgI CGANNNNNNTGC 2 cut(s) 892, 926
BciVI GTATCC 1 cut(s) 1292
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 2 cut(s) 207, 208
BcoDI GTCTC 1 cut(s) 632
BcuI ACTAGT 1 cut(s) 316
BfaI CTAG 4 cut(s) 312, 317, 635, 779
BfmI CTRYAG 2 cut(s) 169, 1126
BfoI RGCGCY 1 cut(s) 495
BfuI GTATCC 1 cut(s) 1292
BmcAI AGTACT 1 cut(s) 1004
Bme1390I CCNGG 2 cut(s) 207, 208
Bme18I GGWCC 1 cut(s) 263
BmeT110I CYCGRG 2 cut(s) 206, 1182
BmgT120I GGNCC 2 cut(s) 263, 375
BmiI GGNNCC 3 cut(s) 798, 920, 1147
BmrFI CCNGG 2 cut(s) 207, 208
BmsI GCATC 1 cut(s) 298
BpiI GAAGAC 2 cut(s) 369, 608
BpmI CTGGAG 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 711
BpuMI CCSGG 2 cut(s) 207, 208
Bsa29I ATCGAT 1 cut(s) 892
BsaAI YACGTR 2 cut(s) 1093, 1232
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 206, 207
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 1130, 1160
Bsc4I CCNNNNNNNGG 6 cut(s) 227, 373, 685, 686, 929, 1111
Bse1I ACTGG 2 cut(s) 486, 1010
Bse3DI GCAATG 2 cut(s) 174, 920
BseCI ATCGAT 1 cut(s) 892
BseDI CCNNGG 2 cut(s) 206, 207
BseGI GGATG 2 cut(s) 505, 1023
BseLI CCNNNNNNNGG 6 cut(s) 227, 373, 685, 686, 929, 1111
BseMI GCAATG 2 cut(s) 174, 920
BseMII CTCAG 1 cut(s) 1268
BseNI ACTGG 2 cut(s) 486, 1010
BseRI GAGGAG 1 cut(s) 399
BshFI GGCC 2 cut(s) 21, 377
BshVI ATCGAT 1 cut(s) 892
BsiHKAI GWGCWC 1 cut(s) 1040
BsiHKCI CYCGRG 2 cut(s) 206, 1182
BsiSI CCGG 1 cut(s) 207
BsiWI CGTACG 1 cut(s) 1089
BslI CCNNNNNNNGG 6 cut(s) 227, 373, 685, 686, 929, 1111
BsmAI GTCTC 1 cut(s) 632
BsnI GGCC 2 cut(s) 21, 377
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 2 cut(s) 206, 1182
Bsp119I TTCGAA 1 cut(s) 711
Bsp1286I GDGCHC 2 cut(s) 1040, 1106
Bsp143I GATC 5 cut(s) 140, 426, 885, 1132, 1249
BspANI GGCC 2 cut(s) 21, 377
BspCNI CTCAG 1 cut(s) 1267
BspDI ATCGAT 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 798, 920, 1147
BspPI GGATC 3 cut(s) 148, 1140, 1244
BspT104I TTCGAA 1 cut(s) 711
BspTNI GGTCTC 1 cut(s) 632
BsrDI GCAATG 2 cut(s) 174, 920
BsrI ACTGG 2 cut(s) 486, 1010
BssECI CCNNGG 2 cut(s) 206, 207
BssMI GATC 5 cut(s) 140, 426, 885, 1132, 1249
Bst4CI ACNGT 6 cut(s) 263, 292, 460, 607, 674, 1290
Bst6I CTCTTC 1 cut(s) 554
BstBAI YACGTR 2 cut(s) 1093, 1232
BstBI TTCGAA 1 cut(s) 711
BstC8I GCNNGC 2 cut(s) 400, 1179
BstDEI CTNAG 1 cut(s) 1254
BstF5I GGATG 2 cut(s) 505, 1023
BstH2I RGCGCY 1 cut(s) 495
BstHHI GCGC 3 cut(s) 487, 494, 529
BstKTI GATC 5 cut(s) 143, 429, 888, 1135, 1252
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 5 cut(s) 140, 426, 885, 1132, 1249
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNSI RCATGY 3 cut(s) 88, 913, 1181
BstSCI CCNGG 2 cut(s) 205, 206
BstSFI CTRYAG 2 cut(s) 169, 1126
BstV2I GAAGAC 2 cut(s) 369, 608
BstX2I RGATCY 2 cut(s) 140, 1132
BstYI RGATCY 2 cut(s) 140, 1132
Bsu15I ATCGAT 1 cut(s) 892
BsuI GTATCC 1 cut(s) 1292
BsuRI GGCC 2 cut(s) 21, 377
BsuTUI ATCGAT 1 cut(s) 892
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 505, 1023
BtsIMutI CAGTG 3 cut(s) 297, 456, 479
Cac8I GCNNGC 2 cut(s) 400, 1179
CfoI GCGC 3 cut(s) 487, 494, 529
Cfr13I GGNCC 2 cut(s) 263, 375
Cfr9I CCCGGG 1 cut(s) 206
ClaI ATCGAT 1 cut(s) 892
CseI GACGC 1 cut(s) 913
Csp6I GTAC 7 cut(s) 259, 333, 1003, 1048, 1072, 1090, 1229
CviAII CATG 7 cut(s) 85, 197, 420, 910, 1081, 1178, 1271
CviJI RGCY 9 cut(s) 21, 36, 149, 286, 377, 402, 776, 1104, 1146
CviKI_1 RGCY 9 cut(s) 21, 36, 149, 286, 377, 402, 776, 1104, 1146
CviQI GTAC 7 cut(s) 259, 333, 1003, 1048, 1072, 1090, 1229
DdeI CTNAG 1 cut(s) 1254
DpnI GATC 5 cut(s) 142, 428, 887, 1134, 1251
DpnII GATC 5 cut(s) 140, 426, 885, 1132, 1249
DraI TTTAAA 1 cut(s) 1207
EaeI YGGCCR 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 1106
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 263
Eco88I CYCGRG 2 cut(s) 206, 1182
EcoT22I ATGCAT 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 1106
FaeI CATG 7 cut(s) 88, 200, 423, 913, 1084, 1181, 1274
FatI CATG 7 cut(s) 84, 196, 419, 909, 1080, 1177, 1270
FauNDI CATATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 885
FblI GTMKAC 1 cut(s) 902
FokI GGATG 2 cut(s) 512, 1030
FriOI GRGCYC 1 cut(s) 1106
FspBI CTAG 4 cut(s) 312, 317, 635, 779
GlaI GCGC 3 cut(s) 486, 493, 528
GsuI CTGGAG 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 495
HaeIII GGCC 2 cut(s) 21, 377
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 913
HhaI GCGC 3 cut(s) 487, 494, 529
Hin1II CATG 7 cut(s) 88, 200, 423, 913, 1084, 1181, 1274
Hin6I GCGC 3 cut(s) 485, 492, 527
HinP1I GCGC 3 cut(s) 485, 492, 527
HincII GTYRAC 2 cut(s) 112, 903
HindII GTYRAC 2 cut(s) 112, 903
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 7 cut(s) 6, 408, 728, 899, 953, 997, 1084
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 1 cut(s) 207
Hpy166II GTNNAC 3 cut(s) 112, 295, 903
Hpy188I TCNGA 8 cut(s) 11, 467, 748, 829, 852, 952, 958, 1257
Hpy188III TCNNGA 7 cut(s) 98, 144, 574, 862, 877, 1062, 1136
Hpy8I GTNNAC 3 cut(s) 112, 295, 903
Hpy99I CGWCG 2 cut(s) 907, 1294
HpyAV CCTTC 3 cut(s) 52, 104, 662
HpyCH4III ACNGT 6 cut(s) 263, 292, 460, 607, 674, 1290
HpyCH4IV ACGT 5 cut(s) 522, 1010, 1092, 1199, 1231
HpyCH4V TGCA 4 cut(s) 179, 200, 913, 1120
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpyF3I CTNAG 1 cut(s) 1254
HpySE526I ACGT 5 cut(s) 522, 1010, 1092, 1199, 1231
Hsp92II CATG 7 cut(s) 88, 200, 423, 913, 1084, 1181, 1274
HspAI GCGC 3 cut(s) 485, 492, 527
Ksp22I TGATCA 1 cut(s) 885
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 5 cut(s) 140, 426, 885, 1132, 1249
LmnI GCTCC 4 cut(s) 146, 395, 694, 1151
LweI GCATC 1 cut(s) 298
MaeI CTAG 4 cut(s) 312, 317, 635, 779
MaeII ACGT 5 cut(s) 522, 1010, 1092, 1199, 1231
MaeIII GTNAC 2 cut(s) 454, 668
MalI GATC 5 cut(s) 142, 428, 887, 1134, 1251
MboI GATC 5 cut(s) 140, 426, 885, 1132, 1249
MflI RGATCY 2 cut(s) 140, 1132
MhlI GDGCHC 2 cut(s) 1040, 1106
MlsI TGGCCA 1 cut(s) 21
MluNI TGGCCA 1 cut(s) 21
MlyI GAGTC 4 cut(s) 417, 908, 962, 1078
MmeI TCCRAC 5 cut(s) 118, 136, 701, 807, 936
Mox20I TGGCCA 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 202
MroXI GAANNNNTTC 2 cut(s) 651, 1262
MscI TGGCCA 1 cut(s) 21
MseI TTAA 5 cut(s) 105, 111, 1053, 1158, 1206
Msp20I TGGCCA 1 cut(s) 21
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 2 cut(s) 207, 208
MwoI GCNNNNNNNGC 1 cut(s) 491
NciI CCSGG 2 cut(s) 207, 208
NdeI CATATG 1 cut(s) 925
NdeII GATC 5 cut(s) 140, 426, 885, 1132, 1249
NlaIII CATG 7 cut(s) 88, 200, 423, 913, 1084, 1181, 1274
NlaIV GGNNCC 3 cut(s) 798, 920, 1147
NmuCI GTSAC 2 cut(s) 454, 668
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 3 cut(s) 88, 913, 1181
NspV TTCGAA 1 cut(s) 711
PaeI GCATGC 1 cut(s) 1181
PaeR7I CTCGAG 1 cut(s) 1182
PciI ACATGT 1 cut(s) 84
PdmI GAANNNNTTC 2 cut(s) 651, 1262
PfeI GAWTC 3 cut(s) 6, 728, 997
Pfl23II CGTACG 1 cut(s) 1089
PflMI CCANNNNNTGG 2 cut(s) 227, 929
PleI GAGTC 4 cut(s) 416, 907, 961, 1078
PpsI GAGTC 4 cut(s) 416, 907, 961, 1078
Ppu21I YACGTR 2 cut(s) 1093, 1232
PscI ACATGT 1 cut(s) 84
PshBI ATTAAT 1 cut(s) 105
PspLI CGTACG 1 cut(s) 1089
PspN4I GGNNCC 3 cut(s) 798, 920, 1147
PspPI GGNCC 2 cut(s) 263, 375
PspXI VCTCGAGB 1 cut(s) 1182
PsuI RGATCY 2 cut(s) 140, 1132
RsaI GTAC 7 cut(s) 260, 334, 1004, 1049, 1073, 1091, 1230
RsaNI GTAC 7 cut(s) 259, 333, 1003, 1048, 1072, 1090, 1229
SalI GTCGAC 1 cut(s) 901
SaqAI TTAA 5 cut(s) 105, 111, 1053, 1158, 1206
Sau3AI GATC 5 cut(s) 140, 426, 885, 1132, 1249
Sau96I GGNCC 2 cut(s) 263, 375
ScaI AGTACT 1 cut(s) 1004
SchI GAGTC 4 cut(s) 417, 908, 962, 1078
ScrFI CCNGG 2 cut(s) 207, 208
SduI GDGCHC 2 cut(s) 1040, 1106
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 2 cut(s) 169, 1126
Sfr274I CTCGAG 1 cut(s) 1182
SfuI TTCGAA 1 cut(s) 711
SinI GGWCC 1 cut(s) 263
SlaI CTCGAG 1 cut(s) 1182
SmaI CCCGGG 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 1182
SmoI CTYRAG 1 cut(s) 1182
SpeI ACTAGT 1 cut(s) 316
SphI GCATGC 1 cut(s) 1181
SspMI CTAG 4 cut(s) 312, 317, 635, 779
StyD4I CCNGG 2 cut(s) 205, 206
TaaI ACNGT 6 cut(s) 263, 292, 460, 607, 674, 1290
TaiI ACGT 5 cut(s) 525, 1013, 1095, 1202, 1234
TaqI TCGA 8 cut(s) 97, 711, 869, 876, 892, 902, 1061, 1183
TatI WGTACW 1 cut(s) 1002
TfiI GAWTC 3 cut(s) 6, 728, 997
Tru1I TTAA 5 cut(s) 105, 111, 1053, 1158, 1206
Tru9I TTAA 5 cut(s) 105, 111, 1053, 1158, 1206
TscAI CASTG 3 cut(s) 297, 463, 486
TseFI GTSAC 2 cut(s) 454, 668
Tsp45I GTSAC 2 cut(s) 454, 668
TspDTI ATGAA 7 cut(s) 34, 138, 185, 408, 541, 953, 1287
TspGWI ACGGA 3 cut(s) 809, 1077, 1089
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 3 cut(s) 297, 463, 486
Van91I CCANNNNNTGG 2 cut(s) 227, 929
VpaK11BI GGWCC 1 cut(s) 263
VspI ATTAAT 1 cut(s) 105
XapI RAATTY 3 cut(s) 278, 1066, 1258
XceI RCATGY 3 cut(s) 88, 913, 1181
XhoI CTCGAG 1 cut(s) 1182
XmaI CCCGGG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 902
XmnI GAANNNNTTC 2 cut(s) 651, 1262
XspI CTAG 4 cut(s) 312, 317, 635, 779
ZrmI AGTACT 1 cut(s) 1004
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.