RchiOBHm_Chr5g0017771

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000956
Physical Location & Seq
Reverse (-)
12378714 .. 12380432
1719 bp
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UTR
Exon/CDS
Intron
PRQ29802

Sequence Viewer

Length: 1419 bp
ATGAAGATACCATGCTCAACACTAGTCTTCACACTCTCAGTCCTTTCCCTTTCAATATCAATCTCATGCGCAACATCAAATCCAATTCTCAATGACTTCTTCCAATGCCTTCCCAAATATTCAAACTCCTCCTATCCAATTTCAGAAGCCATTTTCACCCGAGAAAACACCTATTTTCAATCTGTTCTGTTATCATATATTAGAAACCTCAGACATTGTACACCCACAACTCCAAAACCTTTGGCCATTGTAACAGCCAAACATGAATCCCATGTCCAAGCAACCGTGATGTGTGCAAAACAGCATGGTTTACACATGAGAATCCGAAGTGGTGGCCACGACTACGAGGGCTTATCCTTCATATCATACATTTCAAATACCTCTTATATAGTCCTTGACATGTTTTACCTTCGAGAGATTGATGTTAACATCGCAGATGAAAGTGCTTGGGTTGGATCTGGAGCTATTGTTGGAGAACTATACTATAGCATTGCAGAAAAAAGCAAAGTTCATGCATTTCCTGGGGGAGTTTGCCCAAGTGTTGGTGTCGGTGGCCTTTTTAGTGGTGGTGGGTACGGTCCTACTATGAGAAAATTTGGGCTTACAGTGGACAACATAGTAGATGCTAGACTAGTCAATGTGAATGGTACAATCCTTGATAGAAAATCCATGGGAGAAGACCTATTTTGGGCCATTAGAGGAGGTGGTGGAGCAAGCTTTGGAGTCATTCTTTCATGGAAGATCAAATTGCTTCAAGTTCCACCCGTTGTCACTGTTTTCAGAATTACAAAGACACTGGACCAAGGCGCTGCGGATGTTCTTCACAGATTGCTTCGAATGAGTTGGATTGAATCCACCTTATTCTTTTCCGAATACGCATTTGGAACTCCCATAAAGGCTCTAATAAAAAGACCAACGGAACCACCTCCATTCTTCTTCAAAGGGAAGTCGGATTATGTAAAACAACCAATTCCGAAAGAGGTCTTGAAGTCGATATTCGAGAAGATGATCAATCGATACGAGTCGACGCACATGCAATGGAACCCATATGGTGGAAGAATGAATGAGATTTCCGAGTCGGAAACTCCATTCCCTCACAGAGCAGGAAACCTATTTATGATTCAGTACTGGACGTATTGGATGGAAAAAGGGATTGAGAGCACCAACAAGTACCTTAACTTGTCGAGAAATTTGTACGGAAGTATGACTCCGTACGTGTCCAAGAGCCCAAGAGAGGTCTTGCATAACTATAGGGATCTTGACATAGGCTCCAATATGCTTAACCAGACCAGCATTGAGCATGCTAGAGTTTATGGAAGAACATATTTTAAAGGCAATTTTGAAAGATTGGTACGTGTGAAAAGTGCTGTTGATCCTCAGAATTTTTTCAAGCATGAACAGAGTATCCCACCGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

472

Amino Acids

53.29

Weight (kDa)

9.18

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 80 - 219 2.4e-24 FAD binding domain
BBE PF08031 416 - 470 1.4e-17 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 70
AccB7I CCANNNNNTGG 2 cut(s) 542, 1052
AccI GTMKAC 1 cut(s) 1025
AciI CCGC 2 cut(s) 812, 1412
AclWI GGATC 3 cut(s) 463, 1263, 1367
AcoI YGGCCR 2 cut(s) 243, 334
AcsI RAATTY 3 cut(s) 593, 1189, 1381
AfaI GTAC 8 cut(s) 220, 575, 649, 1127, 1172, 1196, 1214, 1353
AfiI CCNNNNNNNGG 4 cut(s) 542, 688, 1052, 1234
AflIII ACRYGT 3 cut(s) 399, 1215, 1354
AhlI ACTAGT 2 cut(s) 22, 631
AjnI CCWGG 1 cut(s) 520
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AloI GAACNNNNNNTCC 1 cut(s) 1389
AluBI AGCT 2 cut(s) 464, 717
AluI AGCT 2 cut(s) 464, 717
Alw21I GWGCWC 1 cut(s) 1163
AlwI GGATC 3 cut(s) 463, 1263, 1367
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 4 cut(s) 243, 334, 553, 690
ApeKI GCWGC 1 cut(s) 809
ApoI RAATTY 3 cut(s) 593, 1189, 1381
Asp700I GAANNNNTTC 1 cut(s) 1385
AspLEI GCGC 2 cut(s) 71, 809
AspS9I GGNCC 3 cut(s) 578, 690, 799
AsuHPI GGTGA 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 835
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 2 cut(s) 578, 799
BalI TGGCCA 2 cut(s) 245, 336
BanII GRGCYC 1 cut(s) 1229
BbsI GAAGAC 2 cut(s) 19, 684
Bbv12I GWGCWC 1 cut(s) 1163
BbvI GCAGC 1 cut(s) 796
BccI CCATC 1 cut(s) 1135
BcgI CGANNNNNNTGC 2 cut(s) 1015, 1049
BciT130I CCWGG 1 cut(s) 522
BciVI GTATCC 1 cut(s) 1415
BclI TGATCA 1 cut(s) 1008
BcuI ACTAGT 2 cut(s) 22, 631
BfaI CTAG 4 cut(s) 23, 627, 632, 1305
BfmI CTRYAG 3 cut(s) 484, 1249, 1415
BfoI RGCGCY 1 cut(s) 810
BfuI GTATCC 1 cut(s) 1415
BisI GCNGC 1 cut(s) 810
BlsI GCNGC 1 cut(s) 811
BmcAI AGTACT 1 cut(s) 1127
Bme1390I CCNGG 1 cut(s) 522
Bme18I GGWCC 2 cut(s) 578, 799
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 578, 690, 799
BmiI GGNNCC 3 cut(s) 921, 1043, 1270
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 1 cut(s) 613
BpiI GAAGAC 2 cut(s) 19, 684
BpmI CTGGAG 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 835
Bsa29I ATCGAT 1 cut(s) 1015
BsaAI YACGTR 2 cut(s) 1216, 1355
BsaJI CCNNGG 3 cut(s) 521, 669, 802
BsaXI ACNNNNNCTCC 4 cut(s) 453, 483, 1253, 1283
Bsc4I CCNNNNNNNGG 4 cut(s) 542, 688, 1052, 1234
Bse1I ACTGG 2 cut(s) 801, 1133
Bse3DI GCAATG 2 cut(s) 489, 1043
BseBI CCWGG 1 cut(s) 522
BseCI ATCGAT 1 cut(s) 1015
BseDI CCNNGG 3 cut(s) 521, 669, 802
BseGI GGATG 2 cut(s) 820, 1146
BseLI CCNNNNNNNGG 4 cut(s) 542, 688, 1052, 1234
BseMI GCAATG 2 cut(s) 489, 1043
BseMII CTCAG 3 cut(s) 51, 223, 1391
BseNI ACTGG 2 cut(s) 801, 1133
BseRI GAGGAG 2 cut(s) 118, 714
BseXI GCAGC 1 cut(s) 796
BshFI GGCC 4 cut(s) 245, 336, 555, 692
BshVI ATCGAT 1 cut(s) 1015
BsiHKAI GWGCWC 1 cut(s) 1163
BsiHKCI CYCGRG 1 cut(s) 159
BsiWI CGTACG 1 cut(s) 1212
BslI CCNNNNNNNGG 4 cut(s) 542, 688, 1052, 1234
BsnI GGCC 4 cut(s) 245, 336, 555, 692
BsoBI CYCGRG 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 835
Bsp1286I GDGCHC 2 cut(s) 1163, 1229
Bsp1407I TGTACA 1 cut(s) 218
Bsp143I GATC 5 cut(s) 455, 741, 1008, 1255, 1372
Bsp19I CCATGG 1 cut(s) 669
BspACI CCGC 2 cut(s) 812, 1412
BspANI GGCC 4 cut(s) 245, 336, 555, 692
BspCNI CTCAG 3 cut(s) 50, 222, 1390
BspDI ATCGAT 1 cut(s) 1015
BspLI GGNNCC 3 cut(s) 921, 1043, 1270
BspPI GGATC 3 cut(s) 463, 1263, 1367
BspT104I TTCGAA 1 cut(s) 835
BsrDI GCAATG 2 cut(s) 489, 1043
BsrGI TGTACA 1 cut(s) 218
BsrI ACTGG 2 cut(s) 801, 1133
BssECI CCNNGG 3 cut(s) 521, 669, 802
BssMI GATC 5 cut(s) 455, 741, 1008, 1255, 1372
BssT1I CCWWGG 2 cut(s) 669, 802
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 4 cut(s) 286, 578, 607, 775
BstAUI TGTACA 1 cut(s) 218
BstBAI YACGTR 2 cut(s) 1216, 1355
BstBI TTCGAA 1 cut(s) 835
BstC8I GCNNGC 2 cut(s) 715, 1302
BstDEI CTNAG 3 cut(s) 37, 209, 1377
BstDSI CCRYGG 1 cut(s) 669
BstF5I GGATG 2 cut(s) 820, 1146
BstH2I RGCGCY 1 cut(s) 810
BstHHI GCGC 2 cut(s) 71, 809
BstKTI GATC 5 cut(s) 458, 744, 1011, 1258, 1375
BstMBI GATC 5 cut(s) 455, 741, 1008, 1255, 1372
BstNI CCWGG 1 cut(s) 522
BstNSI RCATGY 3 cut(s) 403, 1036, 1304
BstSCI CCNGG 1 cut(s) 520
BstSFI CTRYAG 3 cut(s) 484, 1249, 1415
BstV1I GCAGC 1 cut(s) 796
BstV2I GAAGAC 2 cut(s) 19, 684
BstX2I RGATCY 2 cut(s) 455, 1255
BstYI RGATCY 2 cut(s) 455, 1255
Bsu15I ATCGAT 1 cut(s) 1015
BsuI GTATCC 1 cut(s) 1415
BsuRI GGCC 4 cut(s) 245, 336, 555, 692
BsuTUI ATCGAT 1 cut(s) 1015
BtgI CCRYGG 1 cut(s) 669
BtgZI GCGATG 1 cut(s) 415
BtsCI GGATG 2 cut(s) 820, 1146
BtsIMutI CAGTG 3 cut(s) 612, 771, 794
Cac8I GCNNGC 2 cut(s) 715, 1302
CfoI GCGC 2 cut(s) 71, 809
Cfr13I GGNCC 3 cut(s) 578, 690, 799
ClaI ATCGAT 1 cut(s) 1015
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 8 cut(s) 219, 574, 648, 1126, 1171, 1195, 1213, 1352
CviQI GTAC 8 cut(s) 219, 574, 648, 1126, 1171, 1195, 1213, 1352
DdeI CTNAG 3 cut(s) 37, 209, 1377
DpnI GATC 5 cut(s) 457, 743, 1010, 1257, 1374
DpnII GATC 5 cut(s) 455, 741, 1008, 1255, 1372
DraI TTTAAA 1 cut(s) 1330
EaeI YGGCCR 2 cut(s) 243, 334
Eco130I CCWWGG 2 cut(s) 669, 802
Eco24I GRGCYC 1 cut(s) 1229
Eco47I GGWCC 2 cut(s) 578, 799
Eco88I CYCGRG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 2 cut(s) 669, 802
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 1229
ErhI CCWWGG 2 cut(s) 669, 802
FauNDI CATATG 1 cut(s) 1048
FbaI TGATCA 1 cut(s) 1008
FblI GTMKAC 1 cut(s) 1025
Fnu4HI GCNGC 1 cut(s) 810
FokI GGATG 2 cut(s) 827, 1153
FriOI GRGCYC 1 cut(s) 1229
Fsp4HI GCNGC 1 cut(s) 810
FspBI CTAG 4 cut(s) 23, 627, 632, 1305
FspI TGCGCA 1 cut(s) 70
GlaI GCGC 2 cut(s) 70, 808
GluI GCNGC 1 cut(s) 810
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 810
HaeIII GGCC 4 cut(s) 245, 336, 555, 692
HgaI GACGC 1 cut(s) 1036
HhaI GCGC 2 cut(s) 71, 809
Hin6I GCGC 2 cut(s) 69, 807
HinP1I GCGC 2 cut(s) 69, 807
HincII GTYRAC 2 cut(s) 427, 1026
HindII GTYRAC 2 cut(s) 427, 1026
HindIII AAGCTT 1 cut(s) 715
HinfI GANTC 8 cut(s) 266, 321, 723, 851, 1022, 1076, 1120, 1207
HpaI GTTAAC 1 cut(s) 427
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 5 cut(s) 221, 311, 427, 610, 1026
Hpy188III TCNNGA 6 cut(s) 413, 459, 985, 1000, 1185, 1259
Hpy8I GTNNAC 5 cut(s) 221, 311, 427, 610, 1026
Hpy99I CGWCG 1 cut(s) 1030
HpyAV CCTTC 3 cut(s) 119, 367, 419
HpyCH4III ACNGT 4 cut(s) 286, 578, 607, 775
HpyCH4IV ACGT 3 cut(s) 1133, 1215, 1354
HpyCH4V TGCA 5 cut(s) 296, 494, 515, 1036, 1243
HpyF3I CTNAG 3 cut(s) 37, 209, 1377
HpySE526I ACGT 3 cut(s) 1133, 1215, 1354
HspAI GCGC 2 cut(s) 69, 807
Ksp22I TGATCA 1 cut(s) 1008
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 5 cut(s) 455, 741, 1008, 1255, 1372
LmnI GCTCC 3 cut(s) 461, 710, 1274
LpnPI CCDG 8 cut(s) 444, 507, 534, 782, 1089, 1114, 1298, 1303
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 1 cut(s) 613
MaeI CTAG 4 cut(s) 23, 627, 632, 1305
MaeII ACGT 3 cut(s) 1133, 1215, 1354
MaeIII GTNAC 2 cut(s) 250, 769
MalI GATC 5 cut(s) 457, 743, 1010, 1257, 1374
MboI GATC 5 cut(s) 455, 741, 1008, 1255, 1372
MflI RGATCY 2 cut(s) 455, 1255
MhlI GDGCHC 2 cut(s) 1163, 1229
MlsI TGGCCA 2 cut(s) 245, 336
MluCI AATT 9 cut(s) 84, 138, 593, 746, 783, 969, 1189, 1336, 1381
MluNI TGGCCA 2 cut(s) 245, 336
MlyI GAGTC 4 cut(s) 732, 1031, 1085, 1201
MmeI TCCRAC 5 cut(s) 433, 451, 824, 930, 1059
Mox20I TGGCCA 2 cut(s) 245, 336
Mph1103I ATGCAT 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 1385
MscI TGGCCA 2 cut(s) 245, 336
MseI TTAA 4 cut(s) 426, 1176, 1281, 1329
Msp20I TGGCCA 2 cut(s) 245, 336
MspA1I CMGCKG 1 cut(s) 1414
MspR9I CCNGG 1 cut(s) 522
MvaI CCWGG 1 cut(s) 522
NcoI CCATGG 1 cut(s) 669
NdeI CATATG 1 cut(s) 1048
NdeII GATC 5 cut(s) 455, 741, 1008, 1255, 1372
NlaIV GGNNCC 3 cut(s) 921, 1043, 1270
NmuCI GTSAC 1 cut(s) 769
NsbI TGCGCA 1 cut(s) 70
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 3 cut(s) 403, 1036, 1304
NspV TTCGAA 1 cut(s) 835
PaeI GCATGC 1 cut(s) 1304
PciI ACATGT 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 1385
PfeI GAWTC 4 cut(s) 266, 321, 851, 1120
Pfl23II CGTACG 1 cut(s) 1212
PflMI CCANNNNNTGG 2 cut(s) 542, 1052
PkrI GCNGC 1 cut(s) 811
PleI GAGTC 4 cut(s) 731, 1030, 1084, 1201
PpsI GAGTC 4 cut(s) 731, 1030, 1084, 1201
Ppu21I YACGTR 2 cut(s) 1216, 1355
PscI ACATGT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspLI CGTACG 1 cut(s) 1212
PspN4I GGNNCC 3 cut(s) 921, 1043, 1270
PspPI GGNCC 3 cut(s) 578, 690, 799
PsuI RGATCY 2 cut(s) 455, 1255
RsaI GTAC 8 cut(s) 220, 575, 649, 1127, 1172, 1196, 1214, 1353
RsaNI GTAC 8 cut(s) 219, 574, 648, 1126, 1171, 1195, 1213, 1352
SalI GTCGAC 1 cut(s) 1024
SaqAI TTAA 4 cut(s) 426, 1176, 1281, 1329
SatI GCNGC 1 cut(s) 810
Sau3AI GATC 5 cut(s) 455, 741, 1008, 1255, 1372
Sau96I GGNCC 3 cut(s) 578, 690, 799
ScaI AGTACT 1 cut(s) 1127
SchI GAGTC 4 cut(s) 732, 1031, 1085, 1201
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 2 cut(s) 1163, 1229
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 3 cut(s) 484, 1249, 1415
SfuI TTCGAA 1 cut(s) 835
SinI GGWCC 2 cut(s) 578, 799
SpeI ACTAGT 2 cut(s) 22, 631
SphI GCATGC 1 cut(s) 1304
Sse9I AATT 9 cut(s) 84, 138, 593, 746, 783, 969, 1189, 1336, 1381
SsiI CCGC 2 cut(s) 812, 1412
SspI AATATT 1 cut(s) 119
SspMI CTAG 4 cut(s) 23, 627, 632, 1305
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 2 cut(s) 669, 802
TaaI ACNGT 4 cut(s) 286, 578, 607, 775
TaiI ACGT 3 cut(s) 1136, 1218, 1357
TaqI TCGA 7 cut(s) 412, 835, 992, 999, 1015, 1025, 1184
TasI AATT 9 cut(s) 84, 138, 593, 746, 783, 969, 1189, 1336, 1381
TatI WGTACW 2 cut(s) 218, 1125
TfiI GAWTC 4 cut(s) 266, 321, 851, 1120
Tru1I TTAA 4 cut(s) 426, 1176, 1281, 1329
Tru9I TTAA 4 cut(s) 426, 1176, 1281, 1329
TscAI CASTG 3 cut(s) 612, 778, 801
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 1 cut(s) 809
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 8 cut(s) 17, 279, 349, 453, 500, 723, 1076, 1410
TspGWI ACGGA 3 cut(s) 932, 1200, 1212
TspRI CASTG 3 cut(s) 612, 778, 801
Van91I CCANNNNNTGG 2 cut(s) 542, 1052
VpaK11BI GGWCC 2 cut(s) 578, 799
XapI RAATTY 3 cut(s) 593, 1189, 1381
XceI RCATGY 3 cut(s) 403, 1036, 1304
XmiI GTMKAC 1 cut(s) 1025
XmnI GAANNNNTTC 1 cut(s) 1385
XspI CTAG 4 cut(s) 23, 627, 632, 1305
ZrmI AGTACT 1 cut(s) 1127
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.