MD05G1267200.v1.1

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000956
Physical Location & Seq
Forward (+)
40244955 .. 40246547
1593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1267200.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGAAGACTATACAAAGCCGTATTGCAGTACTAATCTCCATCCTCTCAGTCTTTTCCCTTATACTACCGCGGGCAACTGCAGATCTAATTCTCTCTGACTTCCTCCGATGCCTTCCAAAGCAATCTCAGCCCACCTATCAAATCTTTGAAGCCATTTACACCCGAGACAACACCTCATTTCAATCTGTTTTGCAGCACTACATTAGAAACAGAAGATTTTCTACCCCCACAACACCAAAGCCTTTGGCTATTGTAACTGCCAAGCACGAATCACACGTGCAAGCAACTGTTATTTGTGCAAATCAACATGGGTTGCAGATTAGAATCAGAAGTGGTGGGCATGACTATGAGGGCCTATCATATGTATCGGATGTTCCCTTTGTCATTCTTGACCTACTCAATCTTCGAGCCATTGATATTAAAGTCGCAGATAAGAGCGCTTGGGTTCAGGCTGGGGCAACTATTGGTGAACTGTACTATGAAATTGCCAATAAAAGTAAGGTTCATGGGTTTCCTGCGGGAGCTTGCCTAAGTCTTGGTACCGGTGGCCACTTTAGTGGTGGCGGGTACGGGTACTTGATGAGAAAATATGGGCTTACAGTGGATAATATTGAAGATGCGAAACTAGTTAATGTGAACGGGACAATTCTTGATAGAAAGTCCATGGGAGAAGATCTTTTTTGGGCCATTAGAGGAGGAGGTGGAGCGAGCTTTGGAGTCATTCTGTCGTGGAAGATCAAGTTGGTTCCAGTTCCAGCCAAAGTGACAGTGTTCAATATTCAAAGGACTTTAGAGCAAGGTGCTACGGATGTTATTTACAAGTGGCAATACATAGCTCCCAAGTTGCCCAAAGACATCTTCATCAGAGCAATGCCGCAAGTGAAGAATACTACTAATGGAACGAAGACTGTGGAAGTGTCTTTTATTGGTCACTTTTTGGGACAAACTGCCAATCTTCTTCAATTGCTGAATGCAAGGTTCCCAGAATTGGGTTTACAAAAGAATGATTGTTTTGAAATGAGTTGGCTTGAATCGACTATATTCTGGGCTGACTTCCCAGTTGGAACCCCCATAGACGTTTTACTTGACAGGCCAAATGGGCCAGCAGTGTTCTTCAAAGTCAAGTCTGACTATGTGAAAGAACCAATTCCAAAACAAGGAATAGAATCCATATGGAAGATTATACTTAAGATAGAGAAAGTGTGGATGCAATGGAACCCTTACGGTGGAAGAATGAGTGAGATTTCTGAGTCGGAAACACCATTCCCTCACAGAGCTGGAAACCTATTTTTGATTCAGTACTGGACGTTTTGGGGGGAAGAAGGAGCTGAGACTACCAAGAAGTACGTGGGCTTATCAAGAAAGTTGTATGAAGGAACTACTCCTTTTGTTTCCAAGAGCCCAAGGGAGACCTTCCAAAATTACAGGGATATTGATATTGGCGCCAATCTGGATAATCGGACAAATTTTGAAACTGCTAAAGTTTACGGAAGCAAGTATTTCAAGGGAAATTTTGAAAGATTGGTAAGTGTAAAAACTGCGGTTGATCCCCAAAATTTCTTCAAGCATGAGCAAAGCATCCCACCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

531

Amino Acids

59.64

Weight (kDa)

9.19

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 81 - 217 3.4e-26 FAD binding domain
BBE PF08031 472 - 528 5.3e-18 Berberine and berberine like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 539
AccB1I GGYRCC 2 cut(s) 539, 1442
AccII CGCG 1 cut(s) 70
AciI CCGC 6 cut(s) 68, 70, 518, 564, 875, 1541
AclWI GGATC 1 cut(s) 1541
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 3 cut(s) 1465, 1510, 1555
AcvI CACGTG 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 1443
AfaI GTAC 7 cut(s) 30, 476, 541, 569, 575, 1301, 1346
AfeI AGCGCT 1 cut(s) 439
AfiI CCNNNNNNNGG 4 cut(s) 988, 989, 1157, 1226
AflII CTTAAG 1 cut(s) 1187
AflIII ACRYGT 1 cut(s) 274
AgeI ACCGGT 1 cut(s) 542
AhlI ACTAGT 1 cut(s) 625
AjuI GAANNNNNNNTTGG 4 cut(s) 725, 757, 842, 874
AleI CACNNNNGTG 1 cut(s) 555
AluBI AGCT 5 cut(s) 524, 711, 836, 1277, 1328
AluI AGCT 5 cut(s) 524, 711, 836, 1277, 1328
Alw26I GTCTC 3 cut(s) 159, 1325, 1403
AlwI GGATC 1 cut(s) 1541
Ama87I CYCGRG 1 cut(s) 162
Aor51HI AGCGCT 1 cut(s) 439
AoxI GGCC 5 cut(s) 352, 547, 684, 1091, 1100
ApeKI GCWGC 1 cut(s) 193
ApoI RAATTY 3 cut(s) 1465, 1510, 1555
AsiGI ACCGGT 1 cut(s) 542
Asp700I GAANNNNTTC 2 cut(s) 217, 1146
Asp718I GGTACC 1 cut(s) 539
AspLEI GCGC 2 cut(s) 440, 1445
AspS9I GGNCC 3 cut(s) 352, 684, 1100
AsuHPI GGTGA 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 162
BalI TGGCCA 1 cut(s) 549
BanI GGYRCC 2 cut(s) 539, 1442
BanII GRGCYC 1 cut(s) 1403
BarI GAAGNNNNNNTAC 2 cut(s) 205, 237
BbrPI CACGTG 1 cut(s) 277
BbsI GAAGAC 2 cut(s) 11, 911
BbvI GCAGC 1 cut(s) 205
BccI CCATC 1 cut(s) 47
BceAI ACGGC 1 cut(s) 3
BcoDI GTCTC 3 cut(s) 159, 1325, 1403
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 1 cut(s) 626
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 2 cut(s) 441, 1446
BfrI CTTAAG 1 cut(s) 1187
BglI GCCNNNNNGGC 1 cut(s) 1099
BglII AGATCT 2 cut(s) 82, 673
BisI GCNGC 2 cut(s) 194, 875
BlsI GCNGC 2 cut(s) 195, 876
BmcAI AGTACT 2 cut(s) 30, 1301
BmeT110I CYCGRG 1 cut(s) 162
BmgT120I GGNCC 3 cut(s) 352, 684, 1100
BmiI GGNNCC 6 cut(s) 541, 747, 980, 1066, 1217, 1444
BmrI ACTGGG 1 cut(s) 1052
BmsI GCATC 4 cut(s) 98, 607, 1197, 1587
BmuI ACTGGG 1 cut(s) 1052
BpiI GAAGAC 2 cut(s) 11, 911
BsaAI YACGTR 2 cut(s) 277, 1348
BsaBI GATNNNNATC 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 1443
BsaI GGTCTC 1 cut(s) 1403
BsaJI CCNNGG 3 cut(s) 68, 663, 1403
BsaWI WCCGGW 1 cut(s) 542
Bsc4I CCNNNNNNNGG 4 cut(s) 988, 989, 1157, 1226
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 3 cut(s) 749, 1058, 1307
Bse3DI GCAATG 2 cut(s) 876, 1217
Bse8I GATNNNNATC 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 68, 663, 1403
BseGI GGATG 5 cut(s) 39, 376, 814, 1212, 1578
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 4 cut(s) 988, 989, 1157, 1226
BseMI GCAATG 2 cut(s) 876, 1217
BseMII CTCAG 4 cut(s) 60, 140, 1239, 1320
BseNI ACTGG 3 cut(s) 749, 1058, 1307
BseRI GAGGAG 2 cut(s) 708, 711
BseXI GCAGC 1 cut(s) 205
BseYI CCCAGC 1 cut(s) 452
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 354, 549, 686, 1093, 1102
BshNI GGYRCC 2 cut(s) 539, 1442
BshTI ACCGGT 1 cut(s) 542
BsiHKCI CYCGRG 1 cut(s) 162
BsiSI CCGG 1 cut(s) 543
BslFI GGGAC 2 cut(s) 655, 954
BslI CCNNNNNNNGG 4 cut(s) 988, 989, 1157, 1226
BsmAI GTCTC 3 cut(s) 159, 1325, 1403
BsmFI GGGAC 2 cut(s) 655, 954
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 5 cut(s) 354, 549, 686, 1093, 1102
Bso31I GGTCTC 1 cut(s) 1403
BsoBI CYCGRG 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 1403
Bsp143I GATC 4 cut(s) 82, 673, 735, 1546
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 6 cut(s) 68, 70, 518, 564, 875, 1541
BspANI GGCC 5 cut(s) 354, 549, 686, 1093, 1102
BspCNI CTCAG 4 cut(s) 59, 139, 1240, 1321
BspFNI CGCG 1 cut(s) 70
BspLI GGNNCC 6 cut(s) 541, 747, 980, 1066, 1217, 1444
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 1541
BspT107I GGYRCC 2 cut(s) 539, 1442
BspTI CTTAAG 1 cut(s) 1187
BspTNI GGTCTC 1 cut(s) 1403
BsrDI GCAATG 2 cut(s) 876, 1217
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 3 cut(s) 749, 1058, 1307
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 68, 663, 1403
BssMI GATC 4 cut(s) 82, 673, 735, 1546
BssNI GRCGYC 1 cut(s) 1443
BssT1I CCWWGG 2 cut(s) 663, 1403
Bst4CI ACNGT 6 cut(s) 289, 474, 601, 769, 910, 1226
BstACI GRCGYC 1 cut(s) 1443
BstAFI CTTAAG 1 cut(s) 1187
BstBAI YACGTR 2 cut(s) 277, 1348
BstC8I GCNNGC 5 cut(s) 72, 282, 526, 709, 1104
BstDEI CTNAG 5 cut(s) 46, 126, 530, 1248, 1329
BstDSI CCRYGG 2 cut(s) 68, 663
BstF5I GGATG 5 cut(s) 39, 376, 814, 1212, 1578
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 2 cut(s) 441, 1446
BstHHI GCGC 2 cut(s) 440, 1445
BstKTI GATC 4 cut(s) 85, 676, 738, 1549
BstMAI GTCTC 3 cut(s) 159, 1325, 1403
BstMBI GATC 4 cut(s) 82, 673, 735, 1546
BstMWI GCNNNNNNNGC 2 cut(s) 127, 1099
BstSFI CTRYAG 1 cut(s) 78
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 1 cut(s) 205
BstV2I GAAGAC 2 cut(s) 11, 911
BstX2I RGATCY 2 cut(s) 82, 673
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 2 cut(s) 82, 673
BsuRI GGCC 5 cut(s) 354, 549, 686, 1093, 1102
BtgI CCRYGG 2 cut(s) 68, 663
BtsCI GGATG 5 cut(s) 39, 376, 814, 1212, 1578
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 3 cut(s) 606, 774, 1113
Cac8I GCNNGC 5 cut(s) 72, 282, 526, 709, 1104
CfoI GCGC 2 cut(s) 440, 1445
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 3 cut(s) 352, 684, 1100
Cfr42I CCGCGG 1 cut(s) 71
Csp6I GTAC 7 cut(s) 29, 475, 540, 568, 574, 1300, 1345
CspAI ACCGGT 1 cut(s) 542
CviAII CATG 5 cut(s) 308, 341, 506, 664, 1568
CviQI GTAC 7 cut(s) 29, 475, 540, 568, 574, 1300, 1345
DdeI CTNAG 5 cut(s) 46, 126, 530, 1248, 1329
DinI GGCGCC 1 cut(s) 1444
DpnI GATC 4 cut(s) 84, 675, 737, 1548
DpnII GATC 4 cut(s) 82, 673, 735, 1546
EaeI YGGCCR 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 663, 1403
Eco24I GRGCYC 1 cut(s) 1403
Eco31I GGTCTC 1 cut(s) 1403
Eco47III AGCGCT 1 cut(s) 439
Eco72I CACGTG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 663, 1403
EcoT38I GRGCYC 1 cut(s) 1403
EgeI GGCGCC 1 cut(s) 1444
EheI GGCGCC 1 cut(s) 1444
ErhI CCWWGG 2 cut(s) 663, 1403
FaeI CATG 5 cut(s) 311, 344, 509, 667, 1571
FaqI GGGAC 2 cut(s) 655, 954
FatI CATG 5 cut(s) 307, 340, 505, 663, 1567
FauI CCCGC 3 cut(s) 63, 511, 557
FauNDI CATATG 2 cut(s) 361, 1172
Fnu4HI GCNGC 2 cut(s) 194, 875
FokI GGATG 5 cut(s) 26, 383, 821, 1219, 1565
FriOI GRGCYC 1 cut(s) 1403
Fsp4HI GCNGC 2 cut(s) 194, 875
FspBI CTAG 1 cut(s) 626
GlaI GCGC 2 cut(s) 439, 1444
GluI GCNGC 2 cut(s) 194, 875
GsaI CCCAGC 1 cut(s) 456
HaeII RGCGCY 2 cut(s) 441, 1446
HaeIII GGCC 5 cut(s) 354, 549, 686, 1093, 1102
HapII CCGG 1 cut(s) 543
HhaI GCGC 2 cut(s) 440, 1445
Hin1I GRCGYC 1 cut(s) 1443
Hin1II CATG 5 cut(s) 311, 344, 509, 667, 1571
Hin6I GCGC 2 cut(s) 438, 1443
HinP1I GCGC 2 cut(s) 438, 1443
HinfI GANTC 7 cut(s) 269, 324, 717, 1031, 1166, 1250, 1294
HpaII CCGG 1 cut(s) 543
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 4 cut(s) 470, 637, 995, 1486
Hpy188I TCNGA 9 cut(s) 97, 107, 329, 370, 866, 1129, 1249, 1255, 1461
Hpy188III TCNNGA 4 cut(s) 389, 650, 1359, 1451
Hpy8I GTNNAC 4 cut(s) 470, 637, 995, 1486
HpyAV CCTTC 4 cut(s) 122, 1316, 1367, 1423
HpyCH4III ACNGT 6 cut(s) 289, 474, 601, 769, 910, 1226
HpyCH4IV ACGT 4 cut(s) 276, 1077, 1307, 1347
HpyCH4V TGCA 8 cut(s) 26, 80, 193, 280, 299, 316, 974, 1210
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 1099
HpyF3I CTNAG 5 cut(s) 46, 126, 530, 1248, 1329
HpySE526I ACGT 4 cut(s) 276, 1077, 1307, 1347
Hsp92I GRCGYC 1 cut(s) 1443
Hsp92II CATG 5 cut(s) 311, 344, 509, 667, 1571
HspAI GCGC 2 cut(s) 438, 1443
KasI GGCGCC 1 cut(s) 1442
KpnI GGTACC 1 cut(s) 543
KspI CCGCGG 1 cut(s) 71
Kzo9I GATC 4 cut(s) 82, 673, 735, 1546
LmnI GCTCC 4 cut(s) 521, 704, 841, 1325
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 4 cut(s) 98, 607, 1197, 1587
MaeI CTAG 1 cut(s) 626
MaeII ACGT 4 cut(s) 276, 1077, 1307, 1347
MaeIII GTNAC 3 cut(s) 253, 763, 929
MalI GATC 4 cut(s) 84, 675, 737, 1548
MboI GATC 4 cut(s) 82, 673, 735, 1546
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 2 cut(s) 82, 673
MhlI GDGCHC 1 cut(s) 1403
MlsI TGGCCA 1 cut(s) 549
MluNI TGGCCA 1 cut(s) 549
Mly113I GGCGCC 1 cut(s) 1443
MlyI GAGTC 2 cut(s) 726, 1259
MmeI TCCRAC 2 cut(s) 1042, 1233
MnlI CCTC 8 cut(s) 53, 113, 184, 343, 686, 689, 692, 1278
Mox20I TGGCCA 1 cut(s) 549
MroXI GAANNNNTTC 2 cut(s) 217, 1146
MscI TGGCCA 1 cut(s) 549
MseI TTAA 3 cut(s) 420, 630, 1188
MslI CAYNNNNRTG 2 cut(s) 345, 555
Msp20I TGGCCA 1 cut(s) 549
MspA1I CMGCKG 1 cut(s) 70
MspCI CTTAAG 1 cut(s) 1187
MspI CCGG 1 cut(s) 543
MunI CAATTG 1 cut(s) 962
Mva1269I GAATGC 1 cut(s) 976
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 2 cut(s) 127, 1099
NarI GGCGCC 1 cut(s) 1443
NcoI CCATGG 1 cut(s) 663
NdeI CATATG 2 cut(s) 361, 1172
NdeII GATC 4 cut(s) 82, 673, 735, 1546
NlaIII CATG 5 cut(s) 311, 344, 509, 667, 1571
NlaIV GGNNCC 6 cut(s) 541, 747, 980, 1066, 1217, 1444
NmuCI GTSAC 2 cut(s) 763, 929
OliI CACNNNNGTG 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 1 cut(s) 976
PdmI GAANNNNTTC 2 cut(s) 217, 1146
PfeI GAWTC 5 cut(s) 269, 324, 1031, 1166, 1294
PinAI ACCGGT 1 cut(s) 542
PkrI GCNGC 2 cut(s) 195, 876
PleI GAGTC 2 cut(s) 725, 1258
PluTI GGCGCC 1 cut(s) 1446
PmaCI CACGTG 1 cut(s) 277
PmlI CACGTG 1 cut(s) 277
PpsI GAGTC 2 cut(s) 725, 1258
Ppu21I YACGTR 2 cut(s) 277, 1348
PspCI CACGTG 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 452
PspN4I GGNNCC 6 cut(s) 541, 747, 980, 1066, 1217, 1444
PspPI GGNCC 3 cut(s) 352, 684, 1100
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 82, 673
RsaI GTAC 7 cut(s) 30, 476, 541, 569, 575, 1301, 1346
RsaNI GTAC 7 cut(s) 29, 475, 540, 568, 574, 1300, 1345
RseI CAYNNNNRTG 2 cut(s) 345, 555
SacII CCGCGG 1 cut(s) 71
SaqAI TTAA 3 cut(s) 420, 630, 1188
SatI GCNGC 2 cut(s) 194, 875
Sau3AI GATC 4 cut(s) 82, 673, 735, 1546
Sau96I GGNCC 3 cut(s) 352, 684, 1100
ScaI AGTACT 2 cut(s) 30, 1301
SchI GAGTC 2 cut(s) 726, 1259
SduI GDGCHC 1 cut(s) 1403
SfaNI GCATC 4 cut(s) 98, 607, 1197, 1587
SfcI CTRYAG 1 cut(s) 78
SfiI GGCCNNNNNGGCC 1 cut(s) 1099
SfoI GGCGCC 1 cut(s) 1444
Sfr303I CCGCGG 1 cut(s) 71
SgrBI CCGCGG 1 cut(s) 71
SmiMI CAYNNNNRTG 2 cut(s) 345, 555
SmlI CTYRAG 1 cut(s) 1187
SmoI CTYRAG 1 cut(s) 1187
SpeI ACTAGT 1 cut(s) 625
SsiI CCGC 6 cut(s) 68, 70, 518, 564, 875, 1541
SspDI GGCGCC 1 cut(s) 1442
SspI AATATT 2 cut(s) 610, 778
SspMI CTAG 1 cut(s) 626
StyI CCWWGG 2 cut(s) 663, 1403
TaaI ACNGT 6 cut(s) 289, 474, 601, 769, 910, 1226
TaiI ACGT 4 cut(s) 279, 1080, 1310, 1350
TaqI TCGA 2 cut(s) 406, 1034
TatI WGTACW 3 cut(s) 28, 474, 1299
TauI GCSGC 1 cut(s) 877
TfiI GAWTC 5 cut(s) 269, 324, 1031, 1166, 1294
Tru1I TTAA 3 cut(s) 420, 630, 1188
Tru9I TTAA 3 cut(s) 420, 630, 1188
TscAI CASTG 3 cut(s) 606, 774, 1113
TseFI GTSAC 2 cut(s) 763, 929
TseI GCWGC 1 cut(s) 193
Tsp45I GTSAC 2 cut(s) 763, 929
TspDTI ATGAA 5 cut(s) 17, 494, 495, 850, 1386
TspGWI ACGGA 2 cut(s) 821, 1503
TspRI CASTG 3 cut(s) 606, 774, 1113
Vha464I CTTAAG 1 cut(s) 1187
XapI RAATTY 3 cut(s) 1465, 1510, 1555
XcmI CCANNNNNNNNNTGG 2 cut(s) 557, 1345
XmnI GAANNNNTTC 2 cut(s) 217, 1146
XspI CTAG 1 cut(s) 626
ZrmI AGTACT 2 cut(s) 30, 1301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.