Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000956

Physical Location & Seq

Genomic Coordinates Reverse (-)

33844616 .. 33846253

Gene Structure 1638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1243900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGAAGTTCTTTCCGGTACTTCTCTCGTTCCTTTTGCTCTCAGTTTCATGCGCAACTTCAGCCCCAGTTTCATTTGATGGCTTCCTCCAATGCCTTACCAGAAAAACTCAGTCTACCCTTCCAATCTTTGATTCCATTTATACCACTCAAAACACCTCATATGATTCCATTTTGCATGCACGTATCAATAATCTCGTATATTCTACACCTGAATATCCAAAACCGTTGGCCATAATCGCAGTCAAGAGTGTATCCCACATTCAAGCAACCATTCTCTGTGCAAAACATAATGGATTGCAAGTTAAAACCCGAAGCGGGGGCCATGACTTCGAGGGCATATCCTACGTGTCGGATGTGCCCTTTGTCATGATTGACATGTTCATCCATCTTAATTCCATTGATGTCAATGTTGCCGATGAGAGCGTGTGGGTTCAATCCGGAGCTCTTATTGGACAAATTTACCATGCAATTTCTACCAAAACCCGAGTTTATGGGTTTCCTGCTGGGATTTGTCCAAAAATGGCAGCTGGTGGTCACTTTGGTGGTGGAGGGTATGGAGTTATGATGAGAAAATACGGGCTTGCTATCGATAATATTGTCGATGCTAAGATCATTACTGCGAATGGTAAACTCCTTGATAGAAAGTCAATGGGAGAAGACCTTTTTTGGGCCATCCGAGGAGGTGGTTCACCTAACTTTGGGGTCATTCTTTCATGGAAGCTCAAGTTGGCTCCAGTTCCGGCGAATGTTACCGTGTTCAATGTGAAAAGGACTATAGCACAAGGTGCAACGGATCTCGTTTACAAGTGGCAAAATGTCGCACCCAGGATGCCCAAAGACCTTTTCCTTAGAGCAATGCCACAAGTCAAGAAAAACAGTACCACGGGAGAAAAGACAGTGGAAGTATCGTTCATAGCCCATTTTCTGGGAAGCAGTGACAAGCTTGTTGCATTGTTGAACAAGAGGTTCCCTGAACTGGGGATTCAATCAACAGACTGCCATGAAATGAGTTGGGTTGAATCCATTGTGTTTTGGGCAGACTACCCACTTGGAACGCCAATAAGCGTTCTACTTAACACGACAAACATGGCAGTCTACTCCAAAGGTAAGTCTGACTATGTGAAGGAACCGCTTTCAAAAGACGTTATAGAATCTATCTGGAAGCATATGATCAAGATAGAGAAGATTTTATTGGATATAAACCCTTACGGTGGAAGAATGGCTGAGATTTCGGCGACAGCGACTCCATATCCTCACAGAGCTGGAAACCTATTCCATGTTATGTATTATACGTATTGGACTGAAGCGGGAATTAATGCCACAAACAGGTATGTTAGGTTAACAAGAGAATTGGCAGACATGATGACTCCATATGTCTCGAAGAATCCAAGAGAGGCCTATCAAAACTACAAGGATCTCGACATCGGTGTGAATCAAGATAATCAGACTGACTTTGCAACGGCTGCAGTTTATGGAATGAAGTATTATAAAGATAATTTTGCTAGATTGGTGCTTGTGAAGACCATGATTGATCCTGAAAACTTTTTCAAGAATAAGCAAAGTATCCCAACTCTTCCAAGGGTGGGAGATGGATATATAAGAGGAATCTTACCTCTCATAGAGAGTGCTATTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

546

Amino Acids

60.63

Weight (kDa)

9.06

Isoelectric Point (pI)

32.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	75 - 212	3.3e-22	FAD binding domain
BBE	PF08031	466 - 523	8e-17	Berberine and berberine like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26380 AT1G26390 AT1G26400 AT1G26410 AT1G26420 AT4G20800
fragaria_vesca	FvH4_3g11042 FvH4_3g11043 FvH4_3g11044 FvH4_3g11090
malus_domestica	MD05G1266700.v1.1 MD05G1266800.v1.1 MD05G1267200.v1.1 MD05G1267300.v1.1 MD10G1243900.v1.1 MD10G1244000.v1.1
prunus_persica	Prupe.1G032800_v2.0.a1 Prupe.4G092300_v2.0.a1 Prupe.4G098500_v2.0.a1 Prupe.4G098600_v2.0.a1 Prupe.4G098700_v2.0.a1 Prupe.4G098900_v2.0.a1 Prupe.4G099000_v2.0.a1 Prupe.4G099100_v2.0.a1 Prupe.4G099200_v2.0.a1 Prupe.4G099400_v2.0.a1 Prupe.4G230400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0017771 RchiOBHm_Chr5g0017801 RchiOBHm_Chr5g0017831 RchiOBHm_Chr5g0017861 RchiOBHm_Chr5g0017931 RchiOBHm_Chr5g0017951 RchiOBHm_Chr5g0017981
rosa_laevigata	RLG00000032314 RLG00000032315 RLG00000032316 RLG00000032324 RLG00000032325 RLG00000032328 RLG00000032349
rosa_multiflora	Rmu_sc0003375.1_g000007 Rmu_sc0003834.1_g000007 Rmu_sc0003834.1_g000013 Rmu_sc0003834.1_g000014 Rmu_sc0005929.1_g000010 Rmu_sc0005929.1_g000016 Rmu_sc0011118.1_g000003 Rmu_sc0011171.1_g000007 Rmu_sc0013814.1_g000002 Rmu_sc0014753.1_g000007
rosa_roxburghii	Rroxscaffold_1G00059450 Rroxscaffold_1G00059620 Rroxscaffold_1G00059700 Rroxscaffold_1G00059710
rosa_rugosa	Rorug05G0038600 Rorug05G0038700 Rorug05G0038900 Rorug05G0039200 Rorug05G0039300
rosa_wichuraiana	Rw5G011460 Rw5G011500 Rw5G011520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1488
Acc16I	TGCGCA	1 cut(s)	52
AccB7I	CCANNNNNTGG	1 cut(s)	925
AccI	GTMKAC	2 cut(s)	113, 1095
AccIII	TCCGGA	1 cut(s)	437
AciI	CCGC	3 cut(s)	315, 1130, 1307
AclWI	GGATC	3 cut(s)	801, 1422, 1526
AcoI	YGGCCR	1 cut(s)	228
AcsI	RAATTY	1 cut(s)	456
AcuI	CTGAAG	2 cut(s)	42, 1323
AdeI	CACNNNGTG	1 cut(s)	785
AfaI	GTAC	2 cut(s)	18, 880
AflIII	ACRYGT	2 cut(s)	345, 375
AgsI	TTSAA	8 cut(s)	263, 434, 760, 958, 986, 1019, 1137, 1549
AjnI	CCWGG	1 cut(s)	824
AjuI	GAANNNNNNNTTGG	8 cut(s)	115, 147, 710, 742, 827, 859, 1175, 1207
AleI	CACNNNNGTG	1 cut(s)	540
AloI	GAACNNNNNNTCC	2 cut(s)	893, 925
AluBI	AGCT	5 cut(s)	443, 527, 721, 943, 1262
AluI	AGCT	5 cut(s)	443, 527, 721, 943, 1262
Alw21I	GWGCWC	1 cut(s)	445
Alw26I	GTCTC	1 cut(s)	1381
AlwI	GGATC	3 cut(s)	801, 1422, 1526
Ama87I	CYCGRG	1 cut(s)	483
Aor13HI	TCCGGA	1 cut(s)	437
AoxI	GGCC	4 cut(s)	228, 319, 669, 1395
ApeKI	GCWGC	2 cut(s)	524, 1463
ApoI	RAATTY	1 cut(s)	456
AseI	ATTAAT	1 cut(s)	1314
AspLEI	GCGC	1 cut(s)	53
AspS9I	GGNCC	2 cut(s)	319, 669
AsuHPI	GGTGA	1 cut(s)	681
AvaI	CYCGRG	1 cut(s)	483
BaeGI	GKGCMC	1 cut(s)	360
BalI	TGGCCA	1 cut(s)	230
BanII	GRGCYC	1 cut(s)	445
BbsI	GAAGAC	2 cut(s)	663, 1526
Bbv12I	GWGCWC	1 cut(s)	445
BbvI	GCAGC	2 cut(s)	536, 1450
BccI	CCATC	4 cut(s)	71, 393, 680, 1583
BceAI	ACGGC	1 cut(s)	1476
BciT130I	CCWGG	1 cut(s)	826
BciVI	GTATCC	2 cut(s)	262, 1574
BclI	TGATCA	1 cut(s)	1170
BcoDI	GTCTC	1 cut(s)	1381
BfaI	CTAG	1 cut(s)	1503
BfmI	CTRYAG	2 cut(s)	774, 1464
BfuI	GTATCC	2 cut(s)	262, 1574
BisI	GCNGC	2 cut(s)	525, 1464
BlsI	GCNGC	2 cut(s)	526, 1465
Bme1390I	CCNGG	1 cut(s)	826
BmeT110I	CYCGRG	1 cut(s)	483
BmgT120I	GGNCC	2 cut(s)	319, 669
BmiI	GGNNCC	4 cut(s)	320, 732, 968, 1128
BmrFI	CCNGG	1 cut(s)	826
BmrI	ACTGGG	2 cut(s)	59, 986
BmsI	GCATC	2 cut(s)	592, 819
BmuI	ACTGGG	2 cut(s)	59, 986
BpiI	GAAGAC	2 cut(s)	663, 1526
BpmI	CTGGAG	1 cut(s)	717
BpuEI	CTTGAG	1 cut(s)	707
Bsa29I	ATCGAT	1 cut(s)	588
BsaAI	YACGTR	3 cut(s)	182, 346, 1293
BsaJI	CCNNGG	4 cut(s)	676, 824, 882, 1577
BsaWI	WCCGGW	2 cut(s)	13, 437
BsaXI	ACNNNNNCTCC	2 cut(s)	1228, 1258
Bse1I	ACTGG	3 cut(s)	65, 734, 981
Bse3DI	GCAATG	1 cut(s)	861
BseAI	TCCGGA	1 cut(s)	437
BseBI	CCWGG	1 cut(s)	826
BseCI	ATCGAT	1 cut(s)	588
BseDI	CCNNGG	4 cut(s)	676, 824, 882, 1577
BseGI	GGATG	4 cut(s)	358, 381, 672, 834
BseMI	GCAATG	1 cut(s)	861
BseMII	CTCAG	3 cut(s)	54, 122, 1215
BseNI	ACTGG	3 cut(s)	65, 734, 981
BseRI	GAGGAG	1 cut(s)	693
BseSI	GKGCMC	1 cut(s)	360
BseXI	GCAGC	2 cut(s)	536, 1450
BseYI	CCCAGC	1 cut(s)	503
BshFI	GGCC	4 cut(s)	230, 321, 671, 1397
BshVI	ATCGAT	1 cut(s)	588
BsiHKAI	GWGCWC	1 cut(s)	445
BsiHKCI	CYCGRG	1 cut(s)	483
BsiSI	CCGG	3 cut(s)	14, 438, 740
BsmAI	GTCTC	1 cut(s)	1381
BsnI	GGCC	4 cut(s)	230, 321, 671, 1397
BsoBI	CYCGRG	1 cut(s)	483
Bsp1286I	GDGCHC	2 cut(s)	360, 445
Bsp13I	TCCGGA	1 cut(s)	437
Bsp143I	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
BspACI	CCGC	3 cut(s)	315, 1130, 1307
BspANI	GGCC	4 cut(s)	230, 321, 671, 1397
BspCNI	CTCAG	3 cut(s)	53, 121, 1216
BspDI	ATCGAT	1 cut(s)	588
BspEI	TCCGGA	1 cut(s)	437
BspHI	TCATGA	1 cut(s)	366
BspLI	GGNNCC	4 cut(s)	320, 732, 968, 1128
BspMAI	CTGCAG	1 cut(s)	1468
BspPI	GGATC	3 cut(s)	801, 1422, 1526
BsrDI	GCAATG	1 cut(s)	861
BsrI	ACTGG	3 cut(s)	65, 734, 981
BssECI	CCNNGG	4 cut(s)	676, 824, 882, 1577
BssMI	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
BssT1I	CCWWGG	1 cut(s)	1577
Bst2UI	CCWGG	1 cut(s)	826
Bst4CI	ACNGT	5 cut(s)	225, 754, 878, 898, 1211
Bst6I	CTCTTC	1 cut(s)	1578
BstAPI	GCANNNNNTGC	2 cut(s)	785, 1463
BstBAI	YACGTR	3 cut(s)	182, 346, 1293
BstC8I	GCNNGC	2 cut(s)	177, 582
BstDEI	CTNAG	5 cut(s)	40, 108, 606, 848, 1224
BstDSI	CCRYGG	1 cut(s)	882
BstF5I	GGATG	4 cut(s)	358, 381, 672, 834
BstHHI	GCGC	1 cut(s)	53
BstKTI	GATC	5 cut(s)	612, 796, 1173, 1417, 1534
BstMAI	GTCTC	1 cut(s)	1381
BstMBI	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
BstMWI	GCNNNNNNNGC	4 cut(s)	59, 236, 785, 1463
BstNI	CCWGG	1 cut(s)	826
BstNSI	RCATGY	2 cut(s)	179, 379
BstSCI	CCNGG	1 cut(s)	824
BstSFI	CTRYAG	2 cut(s)	774, 1464
BstSLI	GKGCMC	1 cut(s)	360
BstSNI	TACGTA	1 cut(s)	1293
BstV1I	GCAGC	2 cut(s)	536, 1450
BstV2I	GAAGAC	2 cut(s)	663, 1526
BstX2I	RGATCY	2 cut(s)	793, 1414
BstYI	RGATCY	2 cut(s)	793, 1414
Bsu15I	ATCGAT	1 cut(s)	588
BsuI	GTATCC	2 cut(s)	262, 1574
BsuRI	GGCC	4 cut(s)	230, 321, 671, 1397
BsuTUI	ATCGAT	1 cut(s)	588
BtgI	CCRYGG	1 cut(s)	882
BtsCI	GGATG	4 cut(s)	358, 381, 672, 834
BtsI	GCAGTG	1 cut(s)	940
BtsIMutI	CAGTG	2 cut(s)	903, 940
Cac8I	GCNNGC	2 cut(s)	177, 582
CciI	TCATGA	1 cut(s)	366
CfoI	GCGC	1 cut(s)	53
Cfr13I	GGNCC	2 cut(s)	319, 669
ClaI	ATCGAT	1 cut(s)	588
Csp6I	GTAC	2 cut(s)	17, 879
CviQI	GTAC	2 cut(s)	17, 879
DdeI	CTNAG	5 cut(s)	40, 108, 606, 848, 1224
DpnI	GATC	5 cut(s)	611, 795, 1172, 1416, 1533
DpnII	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
DraIII	CACNNNGTG	1 cut(s)	785
EaeI	YGGCCR	1 cut(s)	228
Eam1104I	CTCTTC	1 cut(s)	1578
EarI	CTCTTC	1 cut(s)	1578
Ecl136II	GAGCTC	1 cut(s)	443
Eco105I	TACGTA	1 cut(s)	1293
Eco130I	CCWWGG	1 cut(s)	1577
Eco147I	AGGCCT	1 cut(s)	1397
Eco24I	GRGCYC	1 cut(s)	445
Eco53kI	GAGCTC	1 cut(s)	443
Eco57I	CTGAAG	2 cut(s)	42, 1323
Eco88I	CYCGRG	1 cut(s)	483
EcoICRI	GAGCTC	1 cut(s)	443
EcoRII	CCWGG	1 cut(s)	824
EcoT14I	CCWWGG	1 cut(s)	1577
EcoT38I	GRGCYC	1 cut(s)	445
ErhI	CCWWGG	1 cut(s)	1577
FauI	CCCGC	2 cut(s)	308, 1300
FauNDI	CATATG	3 cut(s)	160, 1167, 1372
FbaI	TGATCA	1 cut(s)	1170
FblI	GTMKAC	2 cut(s)	113, 1095
Fnu4HI	GCNGC	2 cut(s)	525, 1464
FokI	GGATG	4 cut(s)	365, 368, 659, 841
FriOI	GRGCYC	1 cut(s)	445
Fsp4HI	GCNGC	2 cut(s)	525, 1464
FspBI	CTAG	1 cut(s)	1503
FspI	TGCGCA	1 cut(s)	52
GlaI	GCGC	1 cut(s)	52
GluI	GCNGC	2 cut(s)	525, 1464
GsaI	CCCAGC	1 cut(s)	507
GsuI	CTGGAG	1 cut(s)	717
HaeIII	GGCC	4 cut(s)	230, 321, 671, 1397
HapII	CCGG	3 cut(s)	14, 438, 740
HhaI	GCGC	1 cut(s)	53
Hin6I	GCGC	1 cut(s)	51
HinP1I	GCGC	1 cut(s)	51
HincII	GTYRAC	1 cut(s)	1341
HindII	GTYRAC	1 cut(s)	1341
HindIII	AAGCTT	1 cut(s)	941
HpaI	GTTAAC	1 cut(s)	1341
HpaII	CCGG	3 cut(s)	14, 438, 740
HphI	GGTGA	1 cut(s)	681
Hpy166II	GTNNAC	6 cut(s)	114, 629, 689, 802, 1096, 1341
Hpy188I	TCNGA	4 cut(s)	352, 677, 1114, 1446
Hpy8I	GTNNAC	6 cut(s)	114, 629, 689, 802, 1096, 1341
HpyAV	CCTTC	2 cut(s)	128, 1117
HpyCH4III	ACNGT	5 cut(s)	225, 754, 878, 898, 1211
HpyCH4IV	ACGT	4 cut(s)	181, 345, 1143, 1292
HpyCH4V	TGCA	9 cut(s)	175, 179, 281, 298, 467, 788, 950, 1457, 1466
HpyF10VI	GCNNNNNNNGC	4 cut(s)	59, 236, 785, 1463
HpyF3I	CTNAG	5 cut(s)	40, 108, 606, 848, 1224
HpySE526I	ACGT	4 cut(s)	181, 345, 1143, 1292
HspAI	GCGC	1 cut(s)	51
Kpn2I	TCCGGA	1 cut(s)	437
Ksp22I	TGATCA	1 cut(s)	1170
KspAI	GTTAAC	1 cut(s)	1341
Kzo9I	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
LmnI	GCTCC	2 cut(s)	440, 736
Lsp1109I	GCAGC	2 cut(s)	536, 1450
LweI	GCATC	2 cut(s)	592, 819
MaeI	CTAG	1 cut(s)	1503
MaeII	ACGT	4 cut(s)	181, 345, 1143, 1292
MaeIII	GTNAC	3 cut(s)	533, 748, 935
MalI	GATC	5 cut(s)	611, 795, 1172, 1416, 1533
MboI	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
MboII	GAAGA	6 cut(s)	668, 1195, 1227, 1393, 1531, 1565
MflI	RGATCY	2 cut(s)	793, 1414
MhlI	GDGCHC	2 cut(s)	360, 445
MlsI	TGGCCA	1 cut(s)	230
MluCI	AATT	6 cut(s)	391, 456, 468, 1311, 1349, 1495
MluNI	TGGCCA	1 cut(s)	230
MlyI	GAGTC	2 cut(s)	1237, 1360
MmeI	TCCRAC	1 cut(s)	330
Mox20I	TGGCCA	1 cut(s)	230
MroI	TCCGGA	1 cut(s)	437
MscI	TGGCCA	1 cut(s)	230
MseI	TTAA	5 cut(s)	303, 390, 1074, 1314, 1340
MslI	CAYNNNNRTG	2 cut(s)	540, 1427
Msp20I	TGGCCA	1 cut(s)	230
MspA1I	CMGCKG	1 cut(s)	527
MspI	CCGG	3 cut(s)	14, 438, 740
MspR9I	CCNGG	1 cut(s)	826
MvaI	CCWGG	1 cut(s)	826
MwoI	GCNNNNNNNGC	4 cut(s)	59, 236, 785, 1463
NdeI	CATATG	3 cut(s)	160, 1167, 1372
NdeII	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
NlaIV	GGNNCC	4 cut(s)	320, 732, 968, 1128
NmuCI	GTSAC	2 cut(s)	533, 935
NsbI	TGCGCA	1 cut(s)	52
NspI	RCATGY	2 cut(s)	179, 379
OliI	CACNNNNGTG	1 cut(s)	540
PaeI	GCATGC	1 cut(s)	179
PagI	TCATGA	1 cut(s)	366
PceI	AGGCCT	1 cut(s)	1397
PciI	ACATGT	1 cut(s)	375
PcsI	WCGNNNNNNNCGW	1 cut(s)	1238
PfeI	GAWTC	8 cut(s)	131, 164, 982, 1019, 1151, 1384, 1432, 1605
PflMI	CCANNNNNTGG	1 cut(s)	925
PkrI	GCNGC	2 cut(s)	526, 1465
PleI	GAGTC	2 cut(s)	1237, 1360
PpsI	GAGTC	2 cut(s)	1237, 1360
Ppu21I	YACGTR	3 cut(s)	182, 346, 1293
PscI	ACATGT	1 cut(s)	375
PshBI	ATTAAT	1 cut(s)	1314
PsiI	TTATAA	1 cut(s)	1488
Psp124BI	GAGCTC	1 cut(s)	445
Psp6I	CCWGG	1 cut(s)	824
PspFI	CCCAGC	1 cut(s)	503
PspGI	CCWGG	1 cut(s)	824
PspN4I	GGNNCC	4 cut(s)	320, 732, 968, 1128
PspPI	GGNCC	2 cut(s)	319, 669
PstI	CTGCAG	1 cut(s)	1468
PsuI	RGATCY	2 cut(s)	793, 1414
PvuII	CAGCTG	1 cut(s)	527
RsaI	GTAC	2 cut(s)	18, 880
RsaNI	GTAC	2 cut(s)	17, 879
RseI	CAYNNNNRTG	2 cut(s)	540, 1427
SacI	GAGCTC	1 cut(s)	445
SaqAI	TTAA	5 cut(s)	303, 390, 1074, 1314, 1340
SatI	GCNGC	2 cut(s)	525, 1464
Sau3AI	GATC	5 cut(s)	609, 793, 1170, 1414, 1531
Sau96I	GGNCC	2 cut(s)	319, 669
SchI	GAGTC	2 cut(s)	1237, 1360
ScrFI	CCNGG	1 cut(s)	826
SduI	GDGCHC	2 cut(s)	360, 445
SfaNI	GCATC	2 cut(s)	592, 819
SfcI	CTRYAG	2 cut(s)	774, 1464
SmiMI	CAYNNNNRTG	2 cut(s)	540, 1427
SmlI	CTYRAG	1 cut(s)	722
SmoI	CTYRAG	1 cut(s)	722
SnaBI	TACGTA	1 cut(s)	1293
SphI	GCATGC	1 cut(s)	179
Sse9I	AATT	6 cut(s)	391, 456, 468, 1311, 1349, 1495
SseBI	AGGCCT	1 cut(s)	1397
SsiI	CCGC	3 cut(s)	315, 1130, 1307
SspI	AATATT	1 cut(s)	595
SspMI	CTAG	1 cut(s)	1503
SstI	GAGCTC	1 cut(s)	445
StuI	AGGCCT	1 cut(s)	1397
StyD4I	CCNGG	1 cut(s)	824
StyI	CCWWGG	1 cut(s)	1577
TaaI	ACNGT	5 cut(s)	225, 754, 878, 898, 1211
TaiI	ACGT	4 cut(s)	184, 348, 1146, 1295
TaqI	TCGA	5 cut(s)	330, 588, 600, 1379, 1419
TasI	AATT	6 cut(s)	391, 456, 468, 1311, 1349, 1495
TfiI	GAWTC	8 cut(s)	131, 164, 982, 1019, 1151, 1384, 1432, 1605
Tru1I	TTAA	5 cut(s)	303, 390, 1074, 1314, 1340
Tru9I	TTAA	5 cut(s)	303, 390, 1074, 1314, 1340
TscAI	CASTG	2 cut(s)	903, 940
TseFI	GTSAC	2 cut(s)	533, 935
TseI	GCWGC	2 cut(s)	524, 1463
Tsp45I	GTSAC	2 cut(s)	533, 935
TspDTI	ATGAA	8 cut(s)	17, 36, 60, 370, 702, 901, 1017, 1493
TspGWI	ACGGA	1 cut(s)	806
TspRI	CASTG	2 cut(s)	903, 940
Van91I	CCANNNNNTGG	1 cut(s)	925
VspI	ATTAAT	1 cut(s)	1314
XapI	RAATTY	1 cut(s)	456
XceI	RCATGY	2 cut(s)	179, 379
XmiI	GTMKAC	2 cut(s)	113, 1095
XspI	CTAG	1 cut(s)	1503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.