Rorug05G0038700

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000956
Physical Location & Seq
Reverse (-)
3103167 .. 3108577
5411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0038700.1

Sequence Viewer

Length: 2553 bp
ATGAGAGATTTAGCAAAGCCCCATTGCTTTTCCACCGTTATTCTTCTCCTCACTTTACTCTCTTTCTTCTCCGTCGCCAAATCCAAAGACACCTTGTCTGCAACTGAATCTCTCGGACCAAACCAGACCCTCGTCTCCGCAGGTGAGGTTTTCGAATTGGGTTTCTTCGAGCTAGGCACAGACGTCTCAAGATGGTACTTGGGCATATGGTACAAGAAAATCGAAAAAAAAACTGTTGTTTGGGAAGCCAACAGAGACAACCCGCTCCCAAACAACTCATCCAGTCTCAAAATCGGTTACGATGGAAAACTTTCTCTTGTGGATGAATCCGGAAACGTTTCTTGGTCCAGCAATCAATCACAATTTGTTGGCGTCTCTAAAAATCCAATCCTGCAGCTTTTGGATTCCGGCAATTTGGTCCTCAAAGAAGCAAATGAAATCAACCCTAACAAGTTTCTCTGGCAGAGCTTCGACTATCCCACAGACACTTTGTTGCCGGAGATGAAGCTTGGATGGAACTTGAATACTAGTTTGGACAGATACATATCGTCATGGAAAACTCCAGAAGACCCTTCTACAGGTGATTACTCCTTCAAGCTCGACTACCACGGTTTCCCGGAGGTTTTCCTCCGGCAGAAACAGTCCATAATATACCGGAGCGGGCCCTGGAATGGACTGAGATTTAGCGGCGTTCCAGAGATGAACGCCGGTAATGGTATTGGTTTCAACTTCGTTGTAAATGATGAGGAAGTGTACTACTCGTTTTCGGAACAAGGCAATGAAACTAATCCAGCTCTGAATTCAAGGCTGATCGTAGCTCCCTCCGGCAACATCCAACGGCTGACGTGGATCGAGAGCAGCAAGATCTGGAACAAGTACTGGTTCGCTCCCAAGGACCAATGCGACAGTTACAGCGAGTGCGGGCCCTACGGTGTCTGCGACGCCAACGCTTCGCCAGTGTGTAAGTGCATGAAGGGGTTCCGACCTAAGAACCCTTCGGCGTGGAGCTTGAGAGATGGCTCCGACGGGTGTGAGCGAGAGACGGAGCTGGAGTGTGGGAATAAGGACAAGTTTATGAAGGTGCAGAATGTGAAGCTGCCAGAGAGCGGCGGAGCGGTGGTGGACATGGGGATGAGTTTGGAGGAGTGCAAGGTGAAGTGTTTGGGGAACTGCTCGTGTAGTGGTTATTCGAGTGCGAGGATTCAGAATGGAGGGAGTGGATGTGTGATGTGGTTTGGGGAGTTGATGGATATGAGGAGTTATGCTGACGGCGGGCAAGAGTTCTATCTCCGGTTAGCAGCTTCTGAACTAGATGGTGATGGGAAAACAAAAATAATTATGATCGTCGGCATTGTAGTTGGTATTGCCGTTCTACTATCAGCTGGTCTTATTATCTGTTTTGTGCGGAGGAGGAGGAATTCGGGTAGCACTCTAAATAGGAGAGAATCAAAAGGTCCGCTTGAAAGAAGCCAGGATTTTCTGCTAAACAGTGTGGTAGTCTCAAGTAAGAAGGATCACTACTCTGGTGACAGGAGCAATGATGACCTAGAGTTGCCTTTGTTTGATTTCAGCTCTGTAGTAGCGGCTACGGACAACTTTTCTGATGAAAATAAACTGGGACAAGGAGGTTTCGGTTGTGTTTACAAGGGGTTGGTCGAAGGTCAAGAGATCGCTGTGAAGAGACTTTCAAAAAACTCTGGACAAGGAACTGAAGAATTCAAAAACGAAGTAAGGTTAATTGCAAGACTTCAACACAGAAATCTTGTTCGATTGCTCGGTTGCTGCGTTGATGTGGATGAAAAGATGCTGATTTACGAATACATGGAAAATAAAAGCCTCGATTCTTTTTTATTCGATAAAGCGAAAAGGTATTTGCTGGATTGGCAAAAGCGTTTCAACATTATATGTGGGATTGCTCGAGGACTTCTTTATCTTCATCAGGATTCGAGATTCAGGATCATCCATAGGGACCTCAAGGCAAGCAACATTCTACTTGATGGAGAATTGGACCCCAAGATATCGGACTTTGGAATGGCAAGAATATTTGGTCAGGATCAGACAGAAGCCAATACAAAAAAAGTAGTGGGAACATATGGCTATATGTCTCCTGAATATGCAATGGATGGCCTCTTCTCAATCAAGTCAGATGTATTTAGTTTTGGTGTTCTAGTGTTGGAGATTGTTAGTGGTCAAAAGAACAGAGGATTTTATTATTCAAACAATGAGCTAAACCTCCTAGGACATGCTTGGGAGTTATGGAATGAAGGTCGTGCATTGGATATAATAGATTCTTCAGTTGGTGCTTCATTTTCGGACTCTGAGGTGTTGAGATGCATGCAGGTTGGGCTTTTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTCTGATGTTAAGTAGTGAAAATGCACCACTACCGCAGCCTAAAAACCCCGGTTTTTGCCTAGGAAGGAAACCAGAAACAGAGTCATCCTCAAGCAAGCAAGACGAGTCATTCACTGTAAACCAAGTAACTATCACAATATTAGATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

850

Amino Acids

94.98

Weight (kDa)

5.42

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 186 1.9e-34 D-mannose binding lectin
S_locus_glycop PF00954 218 - 331 9e-35 S-locus glycoprotein domain
PAN_2 PF08276 352 - 418 8.2e-21 PAN-like domain
DUF3660 PF12398 488 - 523 2.1e-09 Receptor serine/threonine kinase
Pkinase PF00069 534 - 732 3.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 536 - 800 1.8e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 805 - 850 5.2e-18 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 131
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 2 cut(s) 131, 2329
AccBSI CCGCTC 4 cut(s) 265, 660, 1107, 1115
AccIII TCCGGA 1 cut(s) 329
AclI AACGTT 1 cut(s) 336
AclWI GGATC 5 cut(s) 857, 1521, 1964, 2061, 2537
AcsI RAATTY 3 cut(s) 799, 1417, 1715
AcuI CTGAAG 2 cut(s) 1731, 2277
AcyI GRCGYC 3 cut(s) 183, 372, 942
AfaI GTAC 4 cut(s) 197, 212, 755, 878
AfiI CCNNNNNNNGG 3 cut(s) 578, 671, 1106
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 846
AjnI CCWGG 2 cut(s) 665, 1470
AjuI GAANNNNNNNTTGG 4 cut(s) 325, 357, 515, 547
Alw26I GTCTC 9 cut(s) 139, 190, 249, 290, 379, 1034, 1504, 1675, 2109
AlwI GGATC 5 cut(s) 857, 1521, 1964, 2061, 2537
AlwNI CAGNNNCTG 1 cut(s) 1304
Ama87I CYCGRG 1 cut(s) 1917
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 4 cut(s) 662, 923, 2125, 2378
ApaI GGGCCC 2 cut(s) 666, 927
ApeKI GCWGC 6 cut(s) 394, 858, 1096, 1298, 1782, 2434
ApoI RAATTY 3 cut(s) 799, 1417, 1715
Asp700I GAANNNNTTC 3 cut(s) 310, 337, 977
AspA2I CCTAGG 3 cut(s) 2236, 2458, 2546
AsuC2I CCSGG 2 cut(s) 617, 2448
AsuHPI GGTGA 5 cut(s) 155, 593, 1165, 1328, 1538
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 1 cut(s) 1917
AvaII GGWCC 7 cut(s) 116, 345, 418, 895, 1454, 1969, 2008
AvrII CCTAGG 3 cut(s) 2236, 2458, 2546
BaeGI GKGCMC 2 cut(s) 666, 927
BanII GRGCYC 2 cut(s) 666, 927
BarI GAAGNNNNNNTAC 2 cut(s) 1502, 1534
BauI CACGAG 1 cut(s) 1174
BbsI GAAGAC 1 cut(s) 573
BbvI GCAGC 6 cut(s) 406, 870, 1083, 1310, 1769, 2446
BccI CCATC 9 cut(s) 186, 296, 507, 1010, 1240, 1307, 1313, 1991, 2117
BceAI ACGGC 3 cut(s) 854, 1285, 1352
BciT130I CCWGG 2 cut(s) 667, 1472
BcnI CCSGG 2 cut(s) 617, 2448
BcoDI GTCTC 9 cut(s) 139, 190, 249, 290, 379, 1034, 1504, 1675, 2109
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 8 cut(s) 173, 528, 1310, 1547, 2168, 2237, 2459, 2547
BfmI CTRYAG 3 cut(s) 392, 576, 1575
BfuAI ACCTGC 2 cut(s) 131, 2329
BglII AGATCT 1 cut(s) 864
BisI GCNGC 9 cut(s) 395, 688, 859, 1097, 1108, 1299, 1584, 1783, 2435
BlnI CCTAGG 3 cut(s) 2236, 2458, 2546
BlsI GCNGC 9 cut(s) 396, 689, 860, 1098, 1109, 1300, 1585, 1784, 2436
BmcAI AGTACT 1 cut(s) 878
Bme1390I CCNGG 4 cut(s) 617, 667, 1472, 2448
Bme18I GGWCC 7 cut(s) 116, 345, 418, 895, 1454, 1969, 2008
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 1917
BmgBI CACGTC 1 cut(s) 846
BmiI GGNNCC 6 cut(s) 664, 925, 980, 1021, 1970, 2010
BmrFI CCNGG 4 cut(s) 617, 667, 1472, 2448
BmrI ACTGGG 1 cut(s) 1625
BmsI GCATC 2 cut(s) 1794, 2321
BmuI ACTGGG 1 cut(s) 1625
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 573
BplI GAGNNNNNCTC 2 cut(s) 2471, 2503
BpmI CTGGAG 2 cut(s) 546, 1070
Bpu14I TTCGAA 1 cut(s) 153
BpuEI CTTGAG 5 cut(s) 172, 1030, 1486, 1958, 2473
BpuMI CCSGG 2 cut(s) 617, 2448
BsaBI GATNNNNATC 1 cut(s) 544
BsaHI GRCGYC 3 cut(s) 183, 372, 942
BsaJI CCNNGG 7 cut(s) 607, 665, 891, 2236, 2446, 2458, 2546
BsaWI WCCGGW 3 cut(s) 329, 654, 1290
BsaXI ACNNNNNCTCC 6 cut(s) 1037, 1067, 1104, 1134, 1207, 1237
Bsc4I CCNNNNNNNGG 3 cut(s) 578, 671, 1106
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 4 cut(s) 282, 884, 956, 1620
Bse3DI GCAATG 4 cut(s) 22, 784, 1543, 2124
Bse8I GATNNNNATC 1 cut(s) 544
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 2 cut(s) 667, 1472
BseDI CCNNGG 7 cut(s) 607, 665, 891, 2236, 2446, 2458, 2546
BseJI GATNNNNATC 1 cut(s) 544
BseLI CCNNNNNNNGG 3 cut(s) 578, 671, 1106
BseMI GCAATG 4 cut(s) 22, 784, 1543, 2124
BseMII CTCAG 2 cut(s) 668, 2310
BseNI ACTGG 4 cut(s) 282, 884, 956, 1620
BseRI GAGGAG 5 cut(s) 38, 1157, 1270, 1423, 1426
BseSI GKGCMC 2 cut(s) 666, 927
BseXI GCAGC 6 cut(s) 406, 870, 1083, 1310, 1769, 2446
BsgI GTGCAG 2 cut(s) 1103, 2388
BshFI GGCC 4 cut(s) 664, 925, 2127, 2380
BsiHKCI CYCGRG 1 cut(s) 1917
BslFI GGGAC 2 cut(s) 1632, 1982
BslI CCNNNNNNNGG 3 cut(s) 578, 671, 1106
BsmAI GTCTC 9 cut(s) 139, 190, 249, 290, 379, 1034, 1504, 1675, 2109
BsmBI CGTCTC 4 cut(s) 139, 190, 379, 1034
BsmFI GGGAC 2 cut(s) 1632, 1982
BsnI GGCC 4 cut(s) 664, 925, 2127, 2380
BsoBI CYCGRG 1 cut(s) 1917
Bsp119I TTCGAA 1 cut(s) 153
Bsp120I GGGCCC 2 cut(s) 662, 923
Bsp1286I GDGCHC 2 cut(s) 666, 927
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
BspANI GGCC 4 cut(s) 664, 925, 2127, 2380
BspCNI CTCAG 2 cut(s) 669, 2311
BspEI TCCGGA 1 cut(s) 329
BspLI GGNNCC 6 cut(s) 664, 925, 980, 1021, 1970, 2010
BspMAI CTGCAG 1 cut(s) 396
BspMI ACCTGC 2 cut(s) 131, 2329
BspPI GGATC 5 cut(s) 857, 1521, 1964, 2061, 2537
BspT104I TTCGAA 1 cut(s) 153
BsrBI CCGCTC 4 cut(s) 265, 660, 1107, 1115
BsrDI GCAATG 4 cut(s) 22, 784, 1543, 2124
BsrFI RCCGGY 1 cut(s) 707
BsrI ACTGG 4 cut(s) 282, 884, 956, 1620
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 7 cut(s) 607, 665, 891, 2236, 2446, 2458, 2546
BssMI GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
BssNI GRCGYC 3 cut(s) 183, 372, 942
BssSI CACGAG 1 cut(s) 1174
BssT1I CCWWGG 4 cut(s) 891, 2236, 2458, 2546
Bst2BI CACGAG 1 cut(s) 1174
Bst2UI CCWGG 2 cut(s) 667, 1472
Bst4CI ACNGT 8 cut(s) 37, 235, 611, 642, 908, 932, 1490, 2515
Bst6I CTCTTC 2 cut(s) 1673, 2135
BstACI GRCGYC 3 cut(s) 183, 372, 942
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 7 cut(s) 662, 923, 1274, 1981, 2336, 2367, 2495
BstDEI CTNAG 3 cut(s) 677, 987, 2319
BstDSI CCRYGG 1 cut(s) 607
BstENI CCTNNNNNAGG 1 cut(s) 576
BstKTI GATC 9 cut(s) 813, 852, 867, 1344, 1516, 1671, 1959, 2056, 2545
BstMAI GTCTC 9 cut(s) 139, 190, 249, 290, 379, 1034, 1504, 1675, 2109
BstMBI GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
BstMWI GCNNNNNNNGC 2 cut(s) 1882, 2344
BstNI CCWGG 2 cut(s) 667, 1472
BstNSI RCATGY 2 cut(s) 2246, 2338
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 4 cut(s) 615, 665, 1470, 2446
BstSFI CTRYAG 3 cut(s) 392, 576, 1575
BstSLI GKGCMC 2 cut(s) 666, 927
BstV1I GCAGC 6 cut(s) 406, 870, 1083, 1310, 1769, 2446
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 2 cut(s) 864, 2542
BstYI RGATCY 2 cut(s) 864, 2542
BsuRI GGCC 4 cut(s) 664, 925, 2127, 2380
BtgI CCRYGG 1 cut(s) 607
BtrI CACGTC 1 cut(s) 846
BtsIMutI CAGTG 3 cut(s) 963, 1495, 2511
BveI ACCTGC 2 cut(s) 131, 2329
Cac8I GCNNGC 7 cut(s) 662, 923, 1274, 1981, 2336, 2367, 2495
CaiI CAGNNNCTG 1 cut(s) 1304
Cfr10I RCCGGY 1 cut(s) 707
CseI GACGC 3 cut(s) 361, 950, 2344
Csp6I GTAC 4 cut(s) 196, 211, 754, 877
CviAII CATG 6 cut(s) 552, 970, 1126, 1822, 2243, 2335
CviQI GTAC 4 cut(s) 196, 211, 754, 877
DdeI CTNAG 3 cut(s) 677, 987, 2319
DpnI GATC 9 cut(s) 812, 851, 866, 1343, 1515, 1670, 1958, 2055, 2544
DpnII GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
DriI GACNNNNNGTC 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 1673, 2135
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 2 cut(s) 1673, 2135
EciI GGCGGA 1 cut(s) 1125
Eco130I CCWWGG 4 cut(s) 891, 2236, 2458, 2546
Eco147I AGGCCT 1 cut(s) 2380
Eco24I GRGCYC 2 cut(s) 666, 927
Eco32I GATATC 1 cut(s) 2019
Eco47I GGWCC 7 cut(s) 116, 345, 418, 895, 1454, 1969, 2008
Eco57I CTGAAG 2 cut(s) 1731, 2277
Eco88I CYCGRG 1 cut(s) 1917
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoO109I RGGNCCY 3 cut(s) 663, 924, 1969
EcoRI GAATTC 3 cut(s) 799, 1417, 1715
EcoRII CCWGG 2 cut(s) 665, 1470
EcoRV GATATC 1 cut(s) 2019
EcoT14I CCWWGG 4 cut(s) 891, 2236, 2458, 2546
EcoT22I ATGCAT 1 cut(s) 2336
EcoT38I GRGCYC 2 cut(s) 666, 927
ErhI CCWWGG 4 cut(s) 891, 2236, 2458, 2546
Esp3I CGTCTC 4 cut(s) 139, 190, 379, 1034
FaeI CATG 6 cut(s) 555, 973, 1129, 1825, 2246, 2338
FalI AAGNNNNNCTT 2 cut(s) 1443, 1475
FaqI GGGAC 2 cut(s) 1632, 1982
FatI CATG 6 cut(s) 551, 969, 1125, 1821, 2242, 2334
FauI CCCGC 4 cut(s) 270, 653, 914, 1265
FauNDI CATATG 2 cut(s) 206, 2092
Fnu4HI GCNGC 9 cut(s) 395, 688, 859, 1097, 1108, 1299, 1584, 1783, 2435
FriOI GRGCYC 2 cut(s) 666, 927
Fsp4HI GCNGC 9 cut(s) 395, 688, 859, 1097, 1108, 1299, 1584, 1783, 2435
FspBI CTAG 8 cut(s) 173, 528, 1310, 1547, 2168, 2237, 2459, 2547
GluI GCNGC 9 cut(s) 395, 688, 859, 1097, 1108, 1299, 1584, 1783, 2435
GsuI CTGGAG 2 cut(s) 546, 1070
HaeIII GGCC 4 cut(s) 664, 925, 2127, 2380
HgaI GACGC 3 cut(s) 361, 950, 2344
Hin1I GRCGYC 3 cut(s) 183, 372, 942
Hin1II CATG 6 cut(s) 555, 973, 1129, 1825, 2246, 2338
HindIII AAGCTT 1 cut(s) 506
HphI GGTGA 5 cut(s) 155, 593, 1165, 1328, 1538
Hpy166II GTNNAC 4 cut(s) 754, 1123, 1642, 2518
Hpy8I GTNNAC 4 cut(s) 754, 1123, 1642, 2518
Hpy99I CGWCG 4 cut(s) 77, 944, 1028, 1349
HpyAV CCTTC 9 cut(s) 582, 601, 967, 1005, 1072, 1504, 1652, 2258, 2457
HpyCH4III ACNGT 8 cut(s) 37, 235, 611, 642, 908, 932, 1490, 2515
HpyCH4IV ACGT 3 cut(s) 183, 336, 845
HpyF10VI GCNNNNNNNGC 2 cut(s) 1882, 2344
HpyF3I CTNAG 3 cut(s) 677, 987, 2319
HpySE526I ACGT 3 cut(s) 183, 336, 845
Hsp92I GRCGYC 3 cut(s) 183, 372, 942
Hsp92II CATG 6 cut(s) 555, 973, 1129, 1825, 2246, 2338
Kpn2I TCCGGA 1 cut(s) 329
Kzo9I GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
LmnI GCTCC 9 cut(s) 270, 657, 823, 892, 1005, 1025, 1045, 1112, 1533
Lsp1109I GCAGC 6 cut(s) 406, 870, 1083, 1310, 1769, 2446
LweI GCATC 2 cut(s) 1794, 2321
MaeI CTAG 8 cut(s) 173, 528, 1310, 1547, 2168, 2237, 2459, 2547
MaeII ACGT 3 cut(s) 183, 336, 845
MaeIII GTNAC 4 cut(s) 296, 908, 1526, 2524
MalI GATC 9 cut(s) 812, 851, 866, 1343, 1515, 1670, 1958, 2055, 2544
MbiI CCGCTC 4 cut(s) 265, 660, 1107, 1115
MboI GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
MflI RGATCY 2 cut(s) 864, 2542
MhlI GDGCHC 2 cut(s) 666, 927
MluCI AATT 9 cut(s) 155, 362, 412, 799, 1335, 1417, 1715, 1737, 2003
MlyI GAGTC 3 cut(s) 2309, 2489, 2513
MmeI TCCRAC 4 cut(s) 859, 1006, 1047, 2154
Mph1103I ATGCAT 1 cut(s) 2336
MroI TCCGGA 1 cut(s) 329
MroXI GAANNNNTTC 3 cut(s) 310, 337, 977
MseI TTAA 2 cut(s) 1736, 2408
MslI CAYNNNNRTG 1 cut(s) 1130
MspA1I CMGCKG 1 cut(s) 1382
MspR9I CCNGG 4 cut(s) 617, 667, 1472, 2448
MvaI CCWGG 2 cut(s) 667, 1472
MwoI GCNNNNNNNGC 2 cut(s) 1882, 2344
NciI CCSGG 2 cut(s) 617, 2448
NdeI CATATG 2 cut(s) 206, 2092
NdeII GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
NlaIII CATG 6 cut(s) 555, 973, 1129, 1825, 2246, 2338
NlaIV GGNNCC 6 cut(s) 664, 925, 980, 1021, 1970, 2010
NmuCI GTSAC 1 cut(s) 1526
NsiI ATGCAT 1 cut(s) 2336
NspI RCATGY 2 cut(s) 2246, 2338
NspV TTCGAA 1 cut(s) 153
PaeI GCATGC 1 cut(s) 2338
PaeR7I CTCGAG 1 cut(s) 1917
PaqCI CACCTGC 1 cut(s) 131
PceI AGGCCT 1 cut(s) 2380
PcsI WCGNNNNNNNCGW 2 cut(s) 936, 1782
PdmI GAANNNNTTC 3 cut(s) 310, 337, 977
PfeI GAWTC 9 cut(s) 107, 326, 404, 1201, 1445, 1841, 1943, 1950, 2288
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 9 cut(s) 396, 689, 860, 1098, 1109, 1300, 1585, 1784, 2436
PleI GAGTC 3 cut(s) 2309, 2488, 2512
PpsI GAGTC 3 cut(s) 2309, 2488, 2512
PpuMI RGGWCCY 1 cut(s) 1969
PshAI GACNNNNGTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 1969
Psp6I CCWGG 2 cut(s) 665, 1470
PspGI CCWGG 2 cut(s) 665, 1470
PspN4I GGNNCC 6 cut(s) 664, 925, 980, 1021, 1970, 2010
PspOMI GGGCCC 2 cut(s) 662, 923
PspPPI RGGWCCY 1 cut(s) 1969
PspXI VCTCGAGB 1 cut(s) 1917
PstI CTGCAG 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 1304
PsuI RGATCY 2 cut(s) 864, 2542
PvuII CAGCTG 1 cut(s) 1382
RsaI GTAC 4 cut(s) 197, 212, 755, 878
RsaNI GTAC 4 cut(s) 196, 211, 754, 877
RseI CAYNNNNRTG 1 cut(s) 1130
SaqAI TTAA 2 cut(s) 1736, 2408
SatI GCNGC 9 cut(s) 395, 688, 859, 1097, 1108, 1299, 1584, 1783, 2435
Sau3AI GATC 9 cut(s) 810, 849, 864, 1341, 1513, 1668, 1956, 2053, 2542
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 3 cut(s) 2309, 2489, 2513
ScrFI CCNGG 4 cut(s) 617, 667, 1472, 2448
SduI GDGCHC 2 cut(s) 666, 927
SfaNI GCATC 2 cut(s) 1794, 2321
SfcI CTRYAG 3 cut(s) 392, 576, 1575
Sfr274I CTCGAG 1 cut(s) 1917
SfuI TTCGAA 1 cut(s) 153
SinI GGWCC 7 cut(s) 116, 345, 418, 895, 1454, 1969, 2008
SlaI CTCGAG 1 cut(s) 1917
SmiMI CAYNNNNRTG 1 cut(s) 1130
SmlI CTYRAG 6 cut(s) 187, 1009, 1501, 1917, 1973, 2488
SmoI CTYRAG 6 cut(s) 187, 1009, 1501, 1917, 1973, 2488
SpeI ACTAGT 1 cut(s) 527
SphI GCATGC 1 cut(s) 2338
Sse9I AATT 9 cut(s) 155, 362, 412, 799, 1335, 1417, 1715, 1737, 2003
SseBI AGGCCT 1 cut(s) 2380
SspI AATATT 2 cut(s) 2043, 2538
SspMI CTAG 8 cut(s) 173, 528, 1310, 1547, 2168, 2237, 2459, 2547
StuI AGGCCT 1 cut(s) 2380
StyD4I CCNGG 4 cut(s) 615, 665, 1470, 2446
StyI CCWWGG 4 cut(s) 891, 2236, 2458, 2546
TaaI ACNGT 8 cut(s) 37, 235, 611, 642, 908, 932, 1490, 2515
TaiI ACGT 3 cut(s) 186, 339, 848
TasI AATT 9 cut(s) 155, 362, 412, 799, 1335, 1417, 1715, 1737, 2003
TatI WGTACW 2 cut(s) 753, 876
TauI GCSGC 3 cut(s) 690, 1110, 1586
TfiI GAWTC 9 cut(s) 107, 326, 404, 1201, 1445, 1841, 1943, 1950, 2288
Tru1I TTAA 2 cut(s) 1736, 2408
Tru9I TTAA 2 cut(s) 1736, 2408
TscAI CASTG 3 cut(s) 963, 1495, 2518
TseFI GTSAC 1 cut(s) 1526
TseI GCWGC 6 cut(s) 394, 858, 1096, 1298, 1782, 2434
Tsp45I GTSAC 1 cut(s) 1526
TspGWI ACGGA 3 cut(s) 61, 1058, 1604
TspRI CASTG 3 cut(s) 963, 1495, 2518
VpaK11BI GGWCC 7 cut(s) 116, 345, 418, 895, 1454, 1969, 2008
XagI CCTNNNNNAGG 1 cut(s) 576
XapI RAATTY 3 cut(s) 799, 1417, 1715
XceI RCATGY 2 cut(s) 2246, 2338
XhoI CTCGAG 1 cut(s) 1917
XmaJI CCTAGG 3 cut(s) 2236, 2458, 2546
XmnI GAANNNNTTC 3 cut(s) 310, 337, 977
XspI CTAG 8 cut(s) 173, 528, 1310, 1547, 2168, 2237, 2459, 2547
ZraI GACGTC 1 cut(s) 184
ZrmI AGTACT 1 cut(s) 878
Zsp2I ATGCAT 1 cut(s) 2336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.