FvH4_3g44820

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000671
Physical Location & Seq
Forward (+)
37307524 .. 37310981
3458 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44820.t1

Sequence Viewer

Length: 3273 bp
ATGGATGTTGATGTCAAGAAGCTAGAGAAACTTTCCTTGATTTCGAAAGTCTGTTCGGAACTTGAAACCAATCTAGGGCCGCAGCATGGAGACAAAATTCTAGCTGAGTTCATAACAGATCTGGGACAGAACAGCAAAAGTGTGGAGGAATTCAATGCCAAATTGAAGGAAAATGGCGCTGCCGAAATCCCTGATTACTTAGTCCGTAATCTTCTCACTATCATTCATGCAGTTCTTGCTCCGGAGAAGAACCCGAGATCTTCCAAAAGAGAAGACGATAAGCCTAAAGATAGAAGCAGAGAGAGCAGAGGTTCTAAGGGAGAAGAAGACAAGCCTAGAGATAGAAGAAGAGAAAGCAGAGGTAATGAGGATCAAGCGTACAATTCCGGTGAGCCAGAGTTGTATGGTGTTTATAAGGGCATGGTTACAAGAGTGATGGATACAGGCTGCTTTGTTGAGTTGAGTTCTCTCAGAGGGAAGGAGGGTCTGGTTCATGTTTCGCAGATGGAGAAGAGGCGAGTTAGTAATGCTAAAGATGTGGTGAAGAGGGGTCATCAAGTTTATGTGAAGGTGGTTTCGATTTCAGGTCAGAAGTTGAGTCTTTCAATGAAAGATGTTGATCAAAAGACAGGGAAGGATTTGCTTCCTATTACGAGGAGTTCAGGCTATGATACTCGTAACTCCAACCCGTTAATGTCAAGGGATGGTACTGCGAGCAGGACTAGTCTTTCTGGGATTAGGATTAAGGAGGATAGTCTTGTCCAGTCTTGTAGGCCAAGGAAGAGACTAAGCTCCCCGGAGAGGTGGGAAATCAAACAGTTGATTGCTTCAGGTGTTTTGAATGCTAAAGATTATGCAGAGATTTATCAAGACGAGGAAGTGGATAGTATAATGATGGTCTATAAAGATGAGGGACCTGAGGAAGAGCTTGAAATTGAGCTCAATGAGGAAGCGCCGGCCTTTTTGCAAGGGCACGACATGTATTCTGTGGATATGTCGCCGGTGAAGATCTTTAAGAATCCAGAAGGATCACTGAGCCGTGCTGCTGCACTTCAATCTGCGCTGGTCAAGGAGCGTAGAGAGATGCGTGATCAGCAGCAAAGAGCTGTGCTGGATTCTATACCGAAGGATTTGAATCGTCCGTGGGAAGATCCAATGCCAGAGAATGGTGAGAGGCATCTTGCACAGGAAATTAGAGGTGTTGGTTTGTCAGCTCATGATGTACCTGAGTGGAAGAAAGAAGCTTATGGGAAGGCTATTAGTTTCGGGAACAGGTCAAGCCTTTCGATTCAGGAACAAAGGAAGAGCTTGCCTATCTACAAGCTGAGGAAAGAAGTGATTGATGCAGTGAATGAGAATCAAGTACTTGTTGTCATTGGTGAGACTGGTTCTGGCAAGACAACACAGCTAACCCAGTATCTTGCAGAAGCAGGTTACACGACGAAGGGTAGAATTGGATGTACACAGCCTCGTAGGGTGGCGGCAATGTCAGTAGCGAAAAGGGTTGCTGAAGAGGTTGGCTGTCGGTTGGGGGAGGAAGTCGGTTATGCCATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACAGATGGTATGCTTCTTAGGGAGATTTTGTTCGATAAAGACCTTATGCAATATTCAGTGATAATGCTTGATGAAGCTCATGAGAGGACAATCCACACAGATGTTCTATTTGGATTACTGAAGCAACTCGTGAACCGCAGACCAGACCTTCGTTTGATTGTTACCTCTGCTACTTTGGATGCAGAAAAGTTTTCAGGTTATTTCAACAATTCCAAGATCTTTACTTTCGAGGGGAGAACTCATCCAGTTGATGTAATGTATGTCAAGGAGCCAGAAAGTGATTACCTTGACGCGGCTATTATCACTGTTTTGCAGATTCACTTGCAAGAGCCAGAAGGTGACATCCTTCTCTTCTTGACTGGACAGGAAGAGATTGATTTTGCATGCCAGGTTCTTTATGAGAGGATGAAAGCTCTTCCTAAAAATATACCCGAGTTGATCATCTTACCGGTTTATAGTGCTCTTCCGAGTGAAATGCAGTCTAGGATATTTGATCTAGCTCCACCAGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCCGAGGCATCTCTGACTATTGATGGAATAATTTATGTCATTGATCCTGGATTTGCAAAGCAGAATGTTTATAACCCAAAGCTGGGGCTAGATTCACTGGTCATAACTCCAATCTCACAAGCATCAGCCAAGCAACGAGCTGGGCGTGCCGGGCGTACAGCCGAGGGGAAATGTTACCGTCTCTACACTGTGAGTGCCTTCAAAAATGAGATGCGTCCTACTACAGTCCCCGAAATCCAGAGGACAAATCTTGCGTCTACGGTGCTTGCAATGAAAGCTATGGGGATAAATGATCTCCTCTCTTTTGATTTTATGGATCCTCCTACGCCGCAGTCACTCATTTCAGCCATGGAGAAACTGTACAGCTTAGGAGCACTTGATGAAGAGGGTTTCATAACCAGACTAGGAATGAAAATGTCTGAGTTTCCTCTAGAACCACCCCTCTCGAAGATGCTACTCGCAAGTGTGGACCTCGGATGCAGTGATGAGATCTTGACCATCATAGCAATGATTCAGACAGGCAATGTGTTTTACAGGCCTAGGGAGAAACAAGACCGCGCTGATCAGAAGAGAGCAAAGTTTTTCCAGCCAGAAGGAGACCATCTTACTTTACTTGCAGTCTACCAGGCTTGGAAAGGAAAAAACTATTCAACACCATGGTGTTTCGAAAACTTTCTTCAGTCTCGCTCCTTGAGGCGCGCGCAGGATGTCCGAAAACAGCTCATGAGCATCATGGACAAGCACAGGCTGGATGTTGTCAGTGCCGGAAACAACTACACAAAGATCACCAAGGCAATCACGGCCGGCTTTTTCTTCCATGTAGCTAGAAAGGATCCACAGGAAGGTTACAAGACCTTTGTAGAGAACCAGCCAGTGTACATCCACCCAAGCAGTGCTCTCTTTCAGAAGCAACCAGGTTGGGTCATCTACAATGAGCTGGTTATGACTACAAAGGAGTACATGCGAGAGGTGACAGCCATTGATCCCAAATGGCTTGTGGAGTTGGCTCCCAGGTTCTTCAAAGCTGCAGATCCTAACAAGATGACCAAGCGCAAGCGTCAACAACGTATCGAACCCTTGTATGACAGATATAATGAACCCAACTCATGGCGCCTTAGTAGACGCCGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1091

Amino Acids

123.5

Weight (kDa)

8.2

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 137 - 203 1.1e-10 S1 RNA binding domain
DEAD PF00270 443 - 593 8.2e-08 DEAD/DEAH box helicase
AAA_22 PF13401 453 - 587 3.3e-06 AAA domain
Helicase_C PF00271 636 - 766 7.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 833 - 857 5.1e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 858 - 917 8.5e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 975 - 1051 9.4e-27 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 414, 2211
Acc36I ACCTGC 1 cut(s) 1421
AccB1I GGYRCC 1 cut(s) 3249
AccB7I CCANNNNNTGG 2 cut(s) 1166, 3246
AccI GTMKAC 3 cut(s) 2396, 2760, 3259
AccII CGCG 4 cut(s) 1892, 2697, 2838, 2840
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 6 cut(s) 80, 1481, 1735, 1892, 2468, 2695
AcoI YGGCCR 1 cut(s) 2940
AcsI RAATTY 2 cut(s) 96, 149
AcuI CTGAAG 4 cut(s) 813, 1530, 1739, 2801
AcyI GRCGYC 2 cut(s) 3250, 3262
AfiI CCNNNNNNNGG 9 cut(s) 75, 86, 801, 1166, 1891, 2221, 2222, 2981, 3246
AflIII ACRYGT 1 cut(s) 978
AgeI ACCGGT 1 cut(s) 2047
AhlI ACTAGT 1 cut(s) 722
AjnI CCWGG 6 cut(s) 1986, 2104, 2185, 2763, 3052, 3149
AleI CACNNNNGTG 1 cut(s) 2797
AloI GAACNNNNNNTCC 2 cut(s) 1613, 1645
Alw21I GWGCWC 4 cut(s) 942, 2062, 2515, 3037
Alw26I GTCTC 6 cut(s) 84, 778, 1376, 2324, 2730, 2826
AlwNI CAGNNNCTG 1 cut(s) 1583
Ama87I CYCGRG 2 cut(s) 253, 2030
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 5 cut(s) 77, 773, 957, 2675, 2940
ApeKI GCWGC 7 cut(s) 82, 179, 447, 1043, 1046, 1096, 3164
ApoI RAATTY 2 cut(s) 96, 149
ArsI GACNNNNNNTTYG 4 cut(s) 1731, 1763, 2378, 2410
AsiGI ACCGGT 1 cut(s) 2047
Asp700I GAANNNNTTC 1 cut(s) 2811
AspA2I CCTAGG 1 cut(s) 2678
AspLEI GCGC 9 cut(s) 179, 955, 1063, 2699, 2838, 2840, 2842, 3192, 3252
AspS9I GGNCC 4 cut(s) 77, 914, 1574, 2608
AsuC2I CCSGG 2 cut(s) 797, 2290
AsuHPI GGTGA 8 cut(s) 401, 553, 1015, 1181, 1391, 1949, 2917, 3121
AsuII TTCGAA 2 cut(s) 44, 2805
AvaI CYCGRG 2 cut(s) 253, 2030
AvaII GGWCC 3 cut(s) 914, 1574, 2608
AvrII CCTAGG 1 cut(s) 2678
AxyI CCTNAGG 1 cut(s) 918
BaeGI GKGCMC 1 cut(s) 975
BamHI GGATCC 2 cut(s) 2455, 2971
BanI GGYRCC 1 cut(s) 3249
BanII GRGCYC 1 cut(s) 942
BauI CACGAG 1 cut(s) 1727
BbsI GAAGAC 2 cut(s) 279, 333
Bbv12I GWGCWC 4 cut(s) 942, 2062, 2515, 3037
BbvCI CCTCAGC 1 cut(s) 1325
BbvI GCAGC 7 cut(s) 94, 166, 434, 1030, 1033, 1108, 3151
BccI CCATC 8 cut(s) 430, 499, 698, 889, 1598, 2156, 2645, 2748
BceAI ACGGC 3 cut(s) 1023, 2955, 3249
BcgI CGANNNNNNTGC 2 cut(s) 2056, 2090
BciT130I CCWGG 6 cut(s) 1988, 2106, 2187, 2765, 3054, 3151
BciVI GTATCC 1 cut(s) 433
BclI TGATCA 4 cut(s) 619, 1090, 2037, 2701
BcnI CCSGG 2 cut(s) 797, 2290
BcoDI GTCTC 6 cut(s) 84, 778, 1376, 2324, 2730, 2826
BcuI ACTAGT 1 cut(s) 722
BfmI CTRYAG 2 cut(s) 2361, 3165
BfoI RGCGCY 3 cut(s) 180, 956, 3253
BfuAI ACCTGC 1 cut(s) 1421
BfuI GTATCC 1 cut(s) 433
BglII AGATCT 5 cut(s) 118, 257, 1008, 1815, 2628
BlnI CCTAGG 1 cut(s) 2678
BmcAI AGTACT 1 cut(s) 1365
Bme1390I CCNGG 8 cut(s) 797, 1988, 2106, 2187, 2290, 2765, 3054, 3151
Bme18I GGWCC 3 cut(s) 914, 1574, 2608
BmeT110I CYCGRG 2 cut(s) 253, 2030
BmgT120I GGNCC 4 cut(s) 77, 914, 1574, 2608
BmiI GGNNCC 6 cut(s) 915, 1869, 2457, 2973, 3147, 3251
BmrFI CCNGG 8 cut(s) 797, 1988, 2106, 2187, 2290, 2765, 3054, 3151
BmrI ACTGGG 1 cut(s) 1408
BmuI ACTGGG 1 cut(s) 1408
BpiI GAAGAC 2 cut(s) 279, 333
Bpu10I CCTNAGC 2 cut(s) 1325, 2506
Bpu14I TTCGAA 2 cut(s) 44, 2805
BpuEI CTTGAG 1 cut(s) 2851
BpuMI CCSGG 2 cut(s) 797, 2290
BsaBI GATNNNNATC 2 cut(s) 618, 1134
BsaHI GRCGYC 2 cut(s) 3250, 3262
BsaI GGTCTC 1 cut(s) 2730
BsaWI WCCGGW 3 cut(s) 241, 386, 2047
BsaXI ACNNNNNCTCC 4 cut(s) 1613, 1643, 2483, 2513
Bsc4I CCNNNNNNNGG 9 cut(s) 75, 86, 801, 1166, 1891, 2221, 2222, 2981, 3246
Bse118I RCCGGY 4 cut(s) 955, 1000, 2047, 2942
Bse1I ACTGG 8 cut(s) 763, 1390, 1414, 1576, 1844, 1963, 2241, 3011
Bse21I CCTNAGG 1 cut(s) 918
Bse3DI GCAATG 4 cut(s) 1491, 2415, 2652, 2668
Bse8I GATNNNNATC 2 cut(s) 618, 1134
BseAI TCCGGA 1 cut(s) 241
BseBI CCWGG 6 cut(s) 1988, 2106, 2187, 2765, 3054, 3151
BseJI GATNNNNATC 2 cut(s) 618, 1134
BseLI CCNNNNNNNGG 9 cut(s) 75, 86, 801, 1166, 1891, 2221, 2222, 2981, 3246
BseMI GCAATG 4 cut(s) 1491, 2415, 2652, 2668
BseMII CTCAG 7 cut(s) 96, 484, 909, 1025, 1218, 1316, 2550
BseNI ACTGG 8 cut(s) 763, 1390, 1414, 1576, 1844, 1963, 2241, 3011
BsePI GCGCGC 2 cut(s) 2836, 2838
BseRI GAGGAG 2 cut(s) 670, 2426
BseSI GKGCMC 1 cut(s) 975
BseX3I CGGCCG 1 cut(s) 2940
BseXI GCAGC 7 cut(s) 94, 166, 434, 1030, 1033, 1108, 3151
BseYI CCCAGC 2 cut(s) 2221, 2279
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 4 cut(s) 1892, 2697, 2838, 2840
Bsh1285I CGRYCG 1 cut(s) 2943
BshFI GGCC 5 cut(s) 79, 775, 959, 2677, 2942
BshNI GGYRCC 1 cut(s) 3249
BshTI ACCGGT 1 cut(s) 2047
BsiEI CGRYCG 1 cut(s) 2943
BsiHKAI GWGCWC 4 cut(s) 942, 2062, 2515, 3037
BsiHKCI CYCGRG 2 cut(s) 253, 2030
BsiSI CCGG 9 cut(s) 242, 387, 797, 956, 1001, 2048, 2289, 2904, 2943
BslFI GGGAC 3 cut(s) 138, 927, 2351
BslI CCNNNNNNNGG 9 cut(s) 75, 86, 801, 1166, 1891, 2221, 2222, 2981, 3246
BsmAI GTCTC 6 cut(s) 84, 778, 1376, 2324, 2730, 2826
BsmBI CGTCTC 1 cut(s) 2324
BsmFI GGGAC 3 cut(s) 138, 927, 2351
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 5 cut(s) 79, 775, 959, 2677, 2942
Bso31I GGTCTC 1 cut(s) 2730
BsoBI CYCGRG 2 cut(s) 253, 2030
Bsp119I TTCGAA 2 cut(s) 44, 2805
Bsp1286I GDGCHC 5 cut(s) 942, 975, 2062, 2515, 3037
Bsp13I TCCGGA 1 cut(s) 241
Bsp1407I TGTACA 3 cut(s) 1460, 2499, 3015
Bsp19I CCATGG 2 cut(s) 2487, 2795
BspACI CCGC 6 cut(s) 80, 1481, 1735, 1892, 2468, 2695
BspANI GGCC 5 cut(s) 79, 775, 959, 2677, 2942
BspCNI CTCAG 7 cut(s) 97, 483, 910, 1026, 1219, 1317, 2551
BspEI TCCGGA 1 cut(s) 241
BspFNI CGCG 4 cut(s) 1892, 2697, 2838, 2840
BspHI TCATGA 3 cut(s) 1216, 1678, 2862
BspLI GGNNCC 6 cut(s) 915, 1869, 2457, 2973, 3147, 3251
BspMAI CTGCAG 1 cut(s) 3169
BspMI ACCTGC 1 cut(s) 1421
BspQI GCTCTTC 4 cut(s) 918, 1298, 2019, 2067
BspT104I TTCGAA 2 cut(s) 44, 2805
BspT107I GGYRCC 1 cut(s) 3249
BspTNI GGTCTC 1 cut(s) 2730
BsrDI GCAATG 4 cut(s) 1491, 2415, 2652, 2668
BsrFI RCCGGY 4 cut(s) 955, 1000, 2047, 2942
BsrGI TGTACA 3 cut(s) 1460, 2499, 3015
BsrI ACTGG 8 cut(s) 763, 1390, 1414, 1576, 1844, 1963, 2241, 3011
BssAI RCCGGY 4 cut(s) 955, 1000, 2047, 2942
BssHII GCGCGC 2 cut(s) 2836, 2838
BssNI GRCGYC 2 cut(s) 3250, 3262
BssSI CACGAG 1 cut(s) 1727
BssT1I CCWWGG 5 cut(s) 776, 2487, 2678, 2795, 2928
Bst2BI CACGAG 1 cut(s) 1727
Bst2UI CCWGG 6 cut(s) 1988, 2106, 2187, 2765, 3054, 3151
Bst4CI ACNGT 8 cut(s) 819, 1585, 1906, 2318, 2329, 2365, 2401, 2499
BstACI GRCGYC 2 cut(s) 3250, 3262
BstAPI GCANNNNNTGC 1 cut(s) 236
BstAUI TGTACA 3 cut(s) 1460, 2499, 3015
BstBI TTCGAA 2 cut(s) 44, 2805
BstDSI CCRYGG 4 cut(s) 1142, 2487, 2795, 3265
BstFNI CGCG 4 cut(s) 1892, 2697, 2838, 2840
BstH2I RGCGCY 3 cut(s) 180, 956, 3253
BstHHI GCGC 9 cut(s) 179, 955, 1063, 2699, 2838, 2840, 2842, 3192, 3252
BstMAI GTCTC 6 cut(s) 84, 778, 1376, 2324, 2730, 2826
BstMCI CGRYCG 1 cut(s) 2943
BstMWI GCNNNNNNNGC 7 cut(s) 236, 303, 1093, 2285, 2290, 2414, 2939
BstNI CCWGG 6 cut(s) 1988, 2106, 2187, 2765, 3054, 3151
BstNSI RCATGY 3 cut(s) 982, 1986, 3103
BstSCI CCNGG 8 cut(s) 795, 1986, 2104, 2185, 2288, 2763, 3052, 3149
BstSFI CTRYAG 2 cut(s) 2361, 3165
BstSLI GKGCMC 1 cut(s) 975
BstUI CGCG 4 cut(s) 1892, 2697, 2838, 2840
BstV1I GCAGC 7 cut(s) 94, 166, 434, 1030, 1033, 1108, 3151
BstV2I GAAGAC 2 cut(s) 279, 333
BstX2I RGATCY 9 cut(s) 118, 257, 1008, 1150, 1815, 2455, 2628, 2971, 3169
BstYI RGATCY 9 cut(s) 118, 257, 1008, 1150, 1815, 2455, 2628, 2971, 3169
BstZI CGGCCG 1 cut(s) 2940
Bsu36I CCTNAGG 1 cut(s) 918
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 5 cut(s) 79, 775, 959, 2677, 2942
BtgI CCRYGG 4 cut(s) 1142, 2487, 2795, 3265
BtsI GCAGTG 3 cut(s) 1353, 2626, 3037
BveI ACCTGC 1 cut(s) 1421
CaiI CAGNNNCTG 1 cut(s) 1583
CciI TCATGA 3 cut(s) 1216, 1678, 2862
CfoI GCGC 9 cut(s) 179, 955, 1063, 2699, 2838, 2840, 2842, 3192, 3252
Cfr10I RCCGGY 4 cut(s) 955, 1000, 2047, 2942
Cfr13I GGNCC 4 cut(s) 77, 914, 1574, 2608
CseI GACGC 4 cut(s) 1898, 2342, 2382, 3185
CsiI ACCWGGT 1 cut(s) 3052
CspAI ACCGGT 1 cut(s) 2047
DinI GGCGCC 1 cut(s) 3251
EaeI YGGCCR 1 cut(s) 2940
EagI CGGCCG 1 cut(s) 2940
Ecl136II GAGCTC 1 cut(s) 940
EclXI CGGCCG 1 cut(s) 2940
Eco130I CCWWGG 5 cut(s) 776, 2487, 2678, 2795, 2928
Eco147I AGGCCT 1 cut(s) 2677
Eco24I GRGCYC 1 cut(s) 942
Eco31I GGTCTC 1 cut(s) 2730
Eco47I GGWCC 3 cut(s) 914, 1574, 2608
Eco52I CGGCCG 1 cut(s) 2940
Eco53kI GAGCTC 1 cut(s) 940
Eco57I CTGAAG 4 cut(s) 813, 1530, 1739, 2801
Eco81I CCTNAGG 1 cut(s) 918
Eco88I CYCGRG 2 cut(s) 253, 2030
EcoICRI GAGCTC 1 cut(s) 940
EcoO109I RGGNCCY 1 cut(s) 914
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 6 cut(s) 1986, 2104, 2185, 2763, 3052, 3149
EcoT14I CCWWGG 5 cut(s) 776, 2487, 2678, 2795, 2928
EcoT38I GRGCYC 1 cut(s) 942
EgeI GGCGCC 1 cut(s) 3251
EheI GGCGCC 1 cut(s) 3251
ErhI CCWWGG 5 cut(s) 776, 2487, 2678, 2795, 2928
Esp3I CGTCTC 1 cut(s) 2324
FaqI GGGAC 3 cut(s) 138, 927, 2351
FbaI TGATCA 4 cut(s) 619, 1090, 2037, 2701
FblI GTMKAC 3 cut(s) 2396, 2760, 3259
FriOI GRGCYC 1 cut(s) 942
GlaI GCGC 9 cut(s) 178, 954, 1062, 2698, 2837, 2839, 2841, 3191, 3251
GsaI CCCAGC 2 cut(s) 2225, 2283
HaeII RGCGCY 3 cut(s) 180, 956, 3253
HaeIII GGCC 5 cut(s) 79, 775, 959, 2677, 2942
HapII CCGG 9 cut(s) 242, 387, 797, 956, 1001, 2048, 2289, 2904, 2943
HgaI GACGC 4 cut(s) 1898, 2342, 2382, 3185
HhaI GCGC 9 cut(s) 179, 955, 1063, 2699, 2838, 2840, 2842, 3192, 3252
Hin1I GRCGYC 2 cut(s) 3250, 3262
Hin6I GCGC 9 cut(s) 177, 953, 1061, 2697, 2836, 2838, 2840, 3190, 3250
HinP1I GCGC 9 cut(s) 177, 953, 1061, 2697, 2836, 2838, 2840, 3190, 3250
HincII GTYRAC 1 cut(s) 3200
HindII GTYRAC 1 cut(s) 3200
HindIII AAGCTT 1 cut(s) 1242
HinfI GANTC 9 cut(s) 598, 1018, 1115, 1135, 1288, 1357, 1915, 2231, 2650
HpaII CCGG 9 cut(s) 242, 387, 797, 956, 1001, 2048, 2289, 2904, 2943
HphI GGTGA 8 cut(s) 401, 553, 1015, 1181, 1391, 1949, 2917, 3121
Hpy166II GTNNAC 8 cut(s) 1463, 1732, 2397, 2608, 2761, 3016, 3200, 3260
Hpy8I GTNNAC 8 cut(s) 1463, 1732, 2397, 2608, 2761, 3016, 3200, 3260
Hpy99I CGWCG 1 cut(s) 1444
HpyCH4III ACNGT 8 cut(s) 819, 1585, 1906, 2318, 2329, 2365, 2401, 2499
HpyCH4IV ACGT 1 cut(s) 3205
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 303, 1093, 2285, 2290, 2414, 2939
HpySE526I ACGT 1 cut(s) 3205
Hsp92I GRCGYC 2 cut(s) 3250, 3262
HspAI GCGC 9 cut(s) 177, 953, 1061, 2697, 2836, 2838, 2840, 3190, 3250
KasI GGCGCC 1 cut(s) 3249
Kpn2I TCCGGA 1 cut(s) 241
KroI GCCGGC 2 cut(s) 955, 2942
KroNI GCCGGC 2 cut(s) 957, 2944
Ksp22I TGATCA 4 cut(s) 619, 1090, 2037, 2701
LguI GCTCTTC 4 cut(s) 918, 1298, 2019, 2067
LmnI GCTCC 8 cut(s) 244, 797, 1072, 1867, 2104, 2510, 2831, 3151
Lsp1109I GCAGC 7 cut(s) 94, 166, 434, 1030, 1033, 1108, 3151
MabI ACCWGGT 1 cut(s) 3052
MaeII ACGT 1 cut(s) 3205
MaeIII GTNAC 9 cut(s) 424, 677, 1433, 1759, 1937, 2312, 2472, 2984, 3109
MflI RGATCY 9 cut(s) 118, 257, 1008, 1150, 1815, 2455, 2628, 2971, 3169
MhlI GDGCHC 5 cut(s) 942, 975, 2062, 2515, 3037
MluCI AATT 9 cut(s) 96, 149, 161, 382, 933, 1191, 1452, 1807, 2169
Mly113I GGCGCC 1 cut(s) 3250
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 1 cut(s) 708
MroI TCCGGA 1 cut(s) 241
MroNI GCCGGC 2 cut(s) 955, 2942
MroXI GAANNNNTTC 1 cut(s) 2811
MseI TTAA 3 cut(s) 692, 744, 1014
MslI CAYNNNNRTG 3 cut(s) 1698, 2645, 2797
MspI CCGG 9 cut(s) 242, 387, 797, 956, 1001, 2048, 2289, 2904, 2943
MspR9I CCNGG 8 cut(s) 797, 1988, 2106, 2187, 2290, 2765, 3054, 3151
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 6 cut(s) 1988, 2106, 2187, 2765, 3054, 3151
MvnI CGCG 4 cut(s) 1892, 2697, 2838, 2840
MwoI GCNNNNNNNGC 7 cut(s) 236, 303, 1093, 2285, 2290, 2414, 2939
NaeI GCCGGC 2 cut(s) 957, 2944
NarI GGCGCC 1 cut(s) 3250
NciI CCSGG 2 cut(s) 797, 2290
NcoI CCATGG 2 cut(s) 2487, 2795
NgoMIV GCCGGC 2 cut(s) 955, 2942
NlaIV GGNNCC 6 cut(s) 915, 1869, 2457, 2973, 3147, 3251
NmeAIII GCCGAG 2 cut(s) 2167, 2326
NmuCI GTSAC 3 cut(s) 1937, 2472, 3109
NspI RCATGY 3 cut(s) 982, 1986, 3103
NspV TTCGAA 2 cut(s) 44, 2805
OliI CACNNNNGTG 1 cut(s) 2797
PaeI GCATGC 1 cut(s) 1986
PagI TCATGA 3 cut(s) 1216, 1678, 2862
PauI GCGCGC 2 cut(s) 2836, 2838
PceI AGGCCT 1 cut(s) 2677
PciI ACATGT 1 cut(s) 978
PciSI GCTCTTC 4 cut(s) 918, 1298, 2019, 2067
PcsI WCGNNNNNNNCGW 1 cut(s) 2281
PctI GAATGC 1 cut(s) 847
PdiI GCCGGC 2 cut(s) 957, 2944
PdmI GAANNNNTTC 1 cut(s) 2811
PfeI GAWTC 8 cut(s) 1018, 1115, 1135, 1288, 1357, 1915, 2231, 2650
PflMI CCANNNNNTGG 2 cut(s) 1166, 3246
PfoI TCCNGGA 1 cut(s) 2185
PinAI ACCGGT 1 cut(s) 2047
PleI GAGTC 1 cut(s) 606
PluTI GGCGCC 1 cut(s) 3253
PpsI GAGTC 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 914
PscI ACATGT 1 cut(s) 978
PsiI TTATAA 2 cut(s) 414, 2211
Psp124BI GAGCTC 1 cut(s) 942
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 6 cut(s) 1986, 2104, 2185, 2763, 3052, 3149
PspFI CCCAGC 2 cut(s) 2221, 2279
PspGI CCWGG 6 cut(s) 1986, 2104, 2185, 2763, 3052, 3149
PspN4I GGNNCC 6 cut(s) 915, 1869, 2457, 2973, 3147, 3251
PspPI GGNCC 4 cut(s) 77, 914, 1574, 2608
PspPPI RGGWCCY 1 cut(s) 914
PstI CTGCAG 1 cut(s) 3169
PstNI CAGNNNCTG 1 cut(s) 1583
PsuI RGATCY 9 cut(s) 118, 257, 1008, 1150, 1815, 2455, 2628, 2971, 3169
PteI GCGCGC 2 cut(s) 2836, 2838
RseI CAYNNNNRTG 3 cut(s) 1698, 2645, 2797
SacI GAGCTC 1 cut(s) 942
SapI GCTCTTC 4 cut(s) 918, 1298, 2019, 2067
SaqAI TTAA 3 cut(s) 692, 744, 1014
Sau96I GGNCC 4 cut(s) 77, 914, 1574, 2608
ScaI AGTACT 1 cut(s) 1365
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 8 cut(s) 797, 1988, 2106, 2187, 2290, 2765, 3054, 3151
SduI GDGCHC 5 cut(s) 942, 975, 2062, 2515, 3037
SexAI ACCWGGT 1 cut(s) 3052
SfcI CTRYAG 2 cut(s) 2361, 3165
SfoI GGCGCC 1 cut(s) 3251
SfuI TTCGAA 2 cut(s) 44, 2805
SgrAI CRCCGGYG 1 cut(s) 1000
SinI GGWCC 3 cut(s) 914, 1574, 2608
SmiMI CAYNNNNRTG 3 cut(s) 1698, 2645, 2797
SmlI CTYRAG 1 cut(s) 2830
SmoI CTYRAG 1 cut(s) 2830
SpeI ACTAGT 1 cut(s) 722
SphI GCATGC 1 cut(s) 1986
Sse9I AATT 9 cut(s) 96, 149, 161, 382, 933, 1191, 1452, 1807, 2169
SseBI AGGCCT 1 cut(s) 2677
SsiI CCGC 6 cut(s) 80, 1481, 1735, 1892, 2468, 2695
SspDI GGCGCC 1 cut(s) 3249
SspI AATATT 2 cut(s) 1652, 2137
SstI GAGCTC 1 cut(s) 942
StuI AGGCCT 1 cut(s) 2677
StyD4I CCNGG 8 cut(s) 795, 1986, 2104, 2185, 2288, 2763, 3052, 3149
StyI CCWWGG 5 cut(s) 776, 2487, 2678, 2795, 2928
TaaI ACNGT 8 cut(s) 819, 1585, 1906, 2318, 2329, 2365, 2401, 2499
TaiI ACGT 1 cut(s) 3208
TaqI TCGA 8 cut(s) 44, 578, 1286, 1632, 1827, 2585, 2805, 3210
TasI AATT 9 cut(s) 96, 149, 161, 382, 933, 1191, 1452, 1807, 2169
TatI WGTACW 6 cut(s) 1363, 1460, 1593, 2499, 3015, 3096
TauI GCSGC 4 cut(s) 82, 1484, 1895, 2470
TfiI GAWTC 8 cut(s) 1018, 1115, 1135, 1288, 1357, 1915, 2231, 2650
Tru1I TTAA 3 cut(s) 692, 744, 1014
Tru9I TTAA 3 cut(s) 692, 744, 1014
TseFI GTSAC 3 cut(s) 1937, 2472, 3109
TseI GCWGC 7 cut(s) 82, 179, 447, 1043, 1046, 1096, 3164
Tsp45I GTSAC 3 cut(s) 1937, 2472, 3109
TspGWI ACGGA 2 cut(s) 194, 1131
Van91I CCANNNNNTGG 2 cut(s) 1166, 3246
VpaK11BI GGWCC 3 cut(s) 914, 1574, 2608
XapI RAATTY 2 cut(s) 96, 149
XbaI TCTAGA 1 cut(s) 2569
XceI RCATGY 3 cut(s) 982, 1986, 3103
XcmI CCANNNNNNNNNTGG 1 cut(s) 3133
XmaJI CCTAGG 1 cut(s) 2678
XmiI GTMKAC 3 cut(s) 2396, 2760, 3259
XmnI GAANNNNTTC 1 cut(s) 2811
ZrmI AGTACT 1 cut(s) 1365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.