RchiOBHm_Chr5g0081521

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000671
Physical Location & Seq
Forward (+)
87502830 .. 87506063
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35577

Sequence Viewer

Length: 3234 bp
ATGGCCGTCAGTGTCAAGAAGCTAGAGTACCTTTCCTTGATTTCAAAAGTCTGTTCGGAACTCGAAACCAATCTAGGGCAGCAGTATGGAGACAAAGCCCTAGCTGAGTTCATAACCGATATGGGACGGAACTCCAAGAACGTGGACGAATTCAATGCCAAGTTGAATGAATTTGGCGCCGAAATCCCTGATTACTTTGTCCGTAATCTTCTCACTATTATTCACGCAGTTCTTGCTCCCAACAAGAACCAGATCTCTTCTAAAGAAGAAAAAGAAGACAAGCCTAGAGATAGAAGTAGAGAAAGCAGAGGTAAGAATAAGGATCAAGAGTACAATTCCGGTGAGCCGGAGTTGTATGGAGTTTATAAGGGCAGGGTTTCGAAAGTGATGGATGCAGGTTGCTTTGTTGAGTTGAGTTGTTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCGAAGAGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAGCAAGTTTATGTGAAGGTGGTTTCGATTTCGGGTCAGAAGTTGAGTCTTTCAATGAAGGATGTTGATCAGAAGACAGGGAAGGATTTACTTCCTTTGAAGAAGAGCTCAGATTATGATGCTACTAGGTCGAACCCGTCAATGTCAAAACATGGTTCTGTGAGTAGGACTAGTCTTTCTGGGATTAGGATTGTGGAGGAGGATAGTTTTGTCCAATCTCGTAGGCCAAGGAAGAGAATGAGCTCCCCAGAGAGGTGGGAAATCAAACAGTTGATTGCTTCAGGTGTTTTGAGTGCTAAAGAGTATCCAATGCTTTATCAAGATGAGGAAGCGGATGGCATAATGATGAAGCAGCTCTATAAAGATGAGGGACCTGAGGAAGAGCTTGAAATCGAGCTCAATGAGGAAGCACCGGTCTTTTTGCAAGGGCACAATATGTATTCTGTGGATATGTCGCCGGTGAAGATCTTTAAGAATCCAGAAGGGTCACTGAGCCGTGCAGCTGCACTTCAATCTGCGCTGATCAAGGAGCGCCGAGAAATGAGAGATCAGCAGCAAAGAGCAGTGCTGGATTCTATCCCGAAGGACCTGAATCGTCCGTGGGAAGATCCAATGGCACAGAATGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCAGCTTATGACATGCCTGAGTGGAAGAAGGATACTTATGCGAAAGCTATGAGTTTCGGGAAGAGGTCAAGCCTCTCGATTCAGGAGCAAAGGAAGAGCTTGCCTGTCTACAAGCTGAGGGAAGAACTGATTGAGGCAGTGAATGAGAATCAAGTGCTTATTGTCATTGGTGAGACTGGTTCTGGTAAGACAACTCAGCTAACACAGTATCTTGCAGAAGCCGGTTACACGACAAAGGGTAGGATTGGATGTACACAGCCTCGTAGGGTGGCGGCAATGTCAGTGGCGAAAAGGGTTGCTGAAGAGGTTGGTTGTCGGTTGGGGGAGGAAGTAGGTTATGCCATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACAGATGGTATGCTTCTTAGGGAGATTTTGATGGATGAGAACCTTTCGCAATACTCTGTGATAATGCTCGATGAAGCTCATGAGAGGACAATCCACACGGATGTTCTTTTTGGATTACTGAAGCAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTTACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCAACTGCATGCTCTTCTCTATCCCTGGGAGAACTTTTCCATACAAGGTAAACTATGTCAAGGAGCCAGAAAGTGATTACCTTGATGCGGCTCTAATCACTGTTTTACAGATCCACTTGGAAGAACCTGAGGGTGACATCCTTCTCTTCCTGACTGGACAGGAAGAGATTGATTTTGCATGTCAGATTCTTTATGAGAGGATGAAAGCTCTTGGTAAAGATGTACCCGAGTTGATTATCCTACCAGTTTATAGTGCTCTTCCTAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCGCCGGGGAAGAGGAAAGTAGTTGTGGCTACTAACATTGCCGAGGCATCTCTGACAATTGATGGAATATTTTATGTCGTTGATCCTGGATTTGCAAAACAAAATGTTTATAACCCAAAGCTGGGGCTAGATTCACTGGTCATAACTCCGATTTCACAAGCATCAGCGAAGCAACGAGCTGGGCGTGCTGGGCGTACAGGCCCTGGGAAATGTTACCGTCTCTACACAGAGAGTGCATTCCGCAATGAGATGCCCCCGACTACAATTCCCGAAATCCAGAGGACAAATCTTGGGTCTACGGTGCTTGCAATGAAAGCAATGGGGATAAATGATCTCCTGTCTTTTGATTTTATGGATCCTCCTGCGCCGCAGGCACTCATTTCAGCAATGGAACAACTGTACAGCTTAGGAGCACTTGATGAAGAGGGTTTCCTAACCAGACTAGGAAGGAAAATGTCAGAGTTTCCTCTGGAGCCACCCCTCTCGAAGATGCTACTCGCCAGTGTGGACCTCGGATGCAGTGATGAGATCCTGACCATCATAGCAATGATTCAGACAGGCAATGTCTTTTACAGGCCTAGGGAGAAACAAGACCGGGCAGATCAGAAGAGAGCAAAGTTTTTCCAGCCAGAAGGAGACCATCTTACTTTACTTGAAGTCTACAAGGCTTGGAAGAGAACAAACTTTTCAGCTCCATGGTGTTTCGACAACTTTCTTCAGTCTCGATCCTTGAGGCGCGCACAGGATGTGAGAAAACAGCTCATGAGCATCATGGATAAGCACAAGCTGGATATTGTCAGTGCCGGAAAAAATTACACAAAGATCAGCAAGGCAATTACGGCCGGCTTTTTCTTCCATGTTGCTAAAAAGGATCCACAAGAAGGTTATAAGACCCTAGTTGAGAGCCAGCCAGTGTACATCCATCCAAGCAGTGCTCTCTTCCAGAAGCAACCAGATTGGGTCATCTACAATGAGCTGGTTATGACTACAAGGGAGTACATGCGCGAGGTGACAGCCATTGATCCCAAATGGCTTGTGGAATTGGCACCAAGGTTCTTCAAAGCTGCAGATCCTAACAAGATGACCAAGCGCAAGCGTCAACAACGTATTGAACCATTGTATGACAGATACAATGAACCCAACTCTTGGCGTCTTACTAGACGCCGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1077

Amino Acids

122.29

Weight (kDa)

8.48

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 121 - 187 1.5e-10 S1 RNA binding domain
DEAD PF00270 431 - 580 3.9e-08 DEAD/DEAH box helicase
AAA_22 PF13401 440 - 571 3.4e-06 AAA domain
Helicase_C PF00271 623 - 754 1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 816 - 844 7.4e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 845 - 904 8.3e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 962 - 1038 7e-27 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 366, 2172, 2949
Acc36I ACCTGC 1 cut(s) 386
AccB1I GGYRCC 2 cut(s) 176, 3106
AccB7I CCANNNNNTGG 1 cut(s) 3207
AccI GTMKAC 3 cut(s) 1278, 2357, 2721
AccII CGCG 2 cut(s) 2799, 3066
AciI CCGC 7 cut(s) 836, 1442, 1696, 1853, 2063, 2302, 2429
AcoI YGGCCR 2 cut(s) 3, 2901
AcsI RAATTY 2 cut(s) 149, 170
AcuI CTGAAG 4 cut(s) 768, 1491, 1700, 2762
AcyI GRCGYC 3 cut(s) 177, 3211, 3223
AfaI GTAC 9 cut(s) 29, 332, 1423, 1556, 1989, 2257, 2462, 2978, 3059
AfiI CCNNNNNNNGG 8 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942, 3207
AgeI ACCGGT 1 cut(s) 916
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 3 cut(s) 1789, 2146, 2263
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
Alw21I GWGCWC 6 cut(s) 614, 749, 903, 2023, 2476, 2998
Alw26I GTCTC 6 cut(s) 84, 1337, 1693, 2285, 2691, 2787
AlwNI CAGNNNCTG 2 cut(s) 1544, 2264
Ama87I CYCGRG 1 cut(s) 1991
AoxI GGCC 5 cut(s) 3, 728, 2260, 2636, 2901
ApeKI GCWGC 6 cut(s) 79, 856, 1004, 1007, 1057, 3125
ApoI RAATTY 2 cut(s) 149, 170
ArsI GACNNNNNNTTYG 4 cut(s) 1692, 1724, 2339, 2371
AsiGI ACCGGT 1 cut(s) 916
AspA2I CCTAGG 1 cut(s) 2639
AspLEI GCGC 8 cut(s) 179, 1024, 1038, 2428, 2799, 2801, 3066, 3153
AspS9I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
AsuC2I CCSGG 2 cut(s) 2067, 2657
AsuHPI GGTGA 7 cut(s) 353, 505, 976, 1142, 1352, 1910, 3082
AsuII TTCGAA 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 1991
AvaII GGWCC 4 cut(s) 875, 1090, 1535, 2569
AvrII CCTAGG 1 cut(s) 2639
AxyI CCTNAGG 2 cut(s) 879, 1893
BaeGI GKGCMC 1 cut(s) 936
BamHI GGATCC 2 cut(s) 2416, 2932
BanI GGYRCC 2 cut(s) 176, 3106
BanII GRGCYC 3 cut(s) 614, 749, 903
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BbsI GAAGAC 2 cut(s) 282, 584
Bbv12I GWGCWC 6 cut(s) 614, 749, 903, 2023, 2476, 2998
BbvCI CCTCAGC 1 cut(s) 1286
BbvI GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 3112
BccI CCATC 8 cut(s) 382, 833, 1559, 1585, 2117, 2606, 2709, 2991
BceAI ACGGC 3 cut(s) 984, 2916, 3210
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 3 cut(s) 1791, 2148, 2265
BciVI GTATCC 2 cut(s) 819, 1195
BclI TGATCA 2 cut(s) 571, 1026
BcnI CCSGG 2 cut(s) 2067, 2657
BcoDI GTCTC 6 cut(s) 84, 1337, 1693, 2285, 2691, 2787
BcuI ACTAGT 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 3126
BfoI RGCGCY 2 cut(s) 180, 1039
BfuAI ACCTGC 1 cut(s) 386
BfuI GTATCC 2 cut(s) 819, 1195
BglII AGATCT 2 cut(s) 252, 969
BisI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1443, 1854, 2429, 3126
BlnI CCTAGG 1 cut(s) 2639
BlsI GCNGC 9 cut(s) 81, 858, 1006, 1009, 1059, 1444, 1855, 2430, 3127
Bme1390I CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
Bme18I GGWCC 4 cut(s) 875, 1090, 1535, 2569
BmeT110I CYCGRG 1 cut(s) 1991
BmgT120I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
BmiI GGNNCC 7 cut(s) 178, 876, 1830, 2418, 2535, 2934, 3108
BmrFI CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
BpiI GAAGAC 2 cut(s) 282, 584
BpmI CTGGAG 1 cut(s) 2552
Bpu10I CCTNAGC 2 cut(s) 1286, 2467
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 2812
BpuMI CCSGG 2 cut(s) 2067, 2657
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 3 cut(s) 177, 3211, 3223
BsaI GGTCTC 2 cut(s) 1693, 2691
BsaWI WCCGGW 2 cut(s) 338, 916
Bsc4I CCNNNNNNNGG 8 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942, 3207
Bse118I RCCGGY 4 cut(s) 916, 961, 1391, 2903
Bse1I ACTGG 7 cut(s) 1351, 1537, 1924, 2009, 2202, 2562, 2972
Bse21I CCTNAGG 2 cut(s) 879, 1893
Bse3DI GCAATG 8 cut(s) 1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 3 cut(s) 1791, 2148, 2265
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 8 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942, 3207
BseMI GCAATG 8 cut(s) 1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
BseMII CTCAG 8 cut(s) 96, 627, 870, 986, 1179, 1277, 1379, 1884
BseNI ACTGG 7 cut(s) 1351, 1537, 1924, 2009, 2202, 2562, 2972
BsePI GCGCGC 1 cut(s) 2797
BseRI GAGGAG 1 cut(s) 716
BseSI GKGCMC 1 cut(s) 936
BseX3I CGGCCG 1 cut(s) 2901
BseXI GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 3112
BseYI CCCAGC 3 cut(s) 2182, 2240, 2249
BsgI GTGCAG 2 cut(s) 993, 1023
Bsh1236I CGCG 2 cut(s) 2799, 3066
Bsh1285I CGRYCG 1 cut(s) 2904
BshFI GGCC 5 cut(s) 5, 730, 2262, 2638, 2903
BshNI GGYRCC 2 cut(s) 176, 3106
BshTI ACCGGT 1 cut(s) 916
BsiEI CGRYCG 1 cut(s) 2904
BsiHKAI GWGCWC 6 cut(s) 614, 749, 903, 2023, 2476, 2998
BsiHKCI CYCGRG 1 cut(s) 1991
BsiSI CCGG 9 cut(s) 339, 347, 917, 962, 1392, 2066, 2656, 2865, 2904
BslFI GGGAC 2 cut(s) 138, 888
BslI CCNNNNNNNGG 8 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942, 3207
BsmAI GTCTC 6 cut(s) 84, 1337, 1693, 2285, 2691, 2787
BsmBI CGTCTC 1 cut(s) 2285
BsmFI GGGAC 2 cut(s) 138, 888
BsmI GAATGC 1 cut(s) 2297
BsnI GGCC 5 cut(s) 5, 730, 2262, 2638, 2903
Bso31I GGTCTC 2 cut(s) 1693, 2691
BsoBI CYCGRG 1 cut(s) 1991
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 7 cut(s) 614, 749, 903, 936, 2023, 2476, 2998
Bsp1407I TGTACA 3 cut(s) 1421, 2460, 2976
Bsp19I CCATGG 1 cut(s) 2756
BspACI CCGC 7 cut(s) 836, 1442, 1696, 1853, 2063, 2302, 2429
BspANI GGCC 5 cut(s) 5, 730, 2262, 2638, 2903
BspCNI CTCAG 8 cut(s) 97, 626, 871, 987, 1180, 1278, 1378, 1885
BspFNI CGCG 2 cut(s) 2799, 3066
BspHI TCATGA 2 cut(s) 1639, 2823
BspLI GGNNCC 7 cut(s) 178, 876, 1830, 2418, 2535, 2934, 3108
BspMAI CTGCAG 1 cut(s) 3130
BspMI ACCTGC 1 cut(s) 386
BspQI GCTCTTC 5 cut(s) 602, 879, 1259, 1784, 2028
BspT104I TTCGAA 1 cut(s) 380
BspT107I GGYRCC 2 cut(s) 176, 3106
BspTNI GGTCTC 2 cut(s) 1693, 2691
BsrDI GCAATG 8 cut(s) 1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
BsrFI RCCGGY 4 cut(s) 916, 961, 1391, 2903
BsrGI TGTACA 3 cut(s) 1421, 2460, 2976
BsrI ACTGG 7 cut(s) 1351, 1537, 1924, 2009, 2202, 2562, 2972
BssAI RCCGGY 4 cut(s) 916, 961, 1391, 2903
BssHII GCGCGC 1 cut(s) 2797
BssNI GRCGYC 3 cut(s) 177, 3211, 3223
BssT1I CCWWGG 4 cut(s) 731, 2639, 2756, 3110
Bst2UI CCWGG 3 cut(s) 1791, 2148, 2265
Bst4CI ACNGT 7 cut(s) 774, 1377, 1546, 1867, 2279, 2362, 2460
BstACI GRCGYC 3 cut(s) 177, 3211, 3223
BstAPI GCANNNNNTGC 1 cut(s) 233
BstAUI TGTACA 3 cut(s) 1421, 2460, 2976
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 9 cut(s) 1271, 1775, 2247, 2367, 2433, 2799, 2905, 2969, 3155
BstDSI CCRYGG 3 cut(s) 1103, 2756, 3226
BstFNI CGCG 2 cut(s) 2799, 3066
BstH2I RGCGCY 2 cut(s) 180, 1039
BstHHI GCGC 8 cut(s) 179, 1024, 1038, 2428, 2799, 2801, 3066, 3153
BstMAI GTCTC 6 cut(s) 84, 1337, 1693, 2285, 2691, 2787
BstMCI CGRYCG 1 cut(s) 2904
BstMWI GCNNNNNNNGC 6 cut(s) 233, 2246, 2251, 2375, 2432, 2900
BstNI CCWGG 3 cut(s) 1791, 2148, 2265
BstNSI RCATGY 4 cut(s) 1186, 1777, 1947, 3064
BstSCI CCNGG 5 cut(s) 1789, 2065, 2146, 2263, 2655
BstSFI CTRYAG 1 cut(s) 3126
BstSLI GKGCMC 1 cut(s) 936
BstUI CGCG 2 cut(s) 2799, 3066
BstV1I GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 3112
BstV2I GAAGAC 2 cut(s) 282, 584
BstX2I RGATCY 8 cut(s) 252, 969, 1111, 1875, 2416, 2589, 2932, 3130
BstXI CCANNNNNNTGG 2 cut(s) 142, 759
BstYI RGATCY 8 cut(s) 252, 969, 1111, 1875, 2416, 2589, 2932, 3130
BstZI CGGCCG 1 cut(s) 2901
Bsu36I CCTNAGG 2 cut(s) 879, 1893
BsuI GTATCC 2 cut(s) 819, 1195
BsuRI GGCC 5 cut(s) 5, 730, 2262, 2638, 2903
BtgI CCRYGG 3 cut(s) 1103, 2756, 3226
BtsI GCAGTG 4 cut(s) 1074, 1314, 2587, 2998
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 9 cut(s) 1271, 1775, 2247, 2367, 2433, 2799, 2905, 2969, 3155
CaiI CAGNNNCTG 2 cut(s) 1544, 2264
CciI TCATGA 2 cut(s) 1639, 2823
CfoI GCGC 8 cut(s) 179, 1024, 1038, 2428, 2799, 2801, 3066, 3153
Cfr10I RCCGGY 4 cut(s) 916, 961, 1391, 2903
Cfr13I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
CseI GACGC 2 cut(s) 3146, 3200
Csp6I GTAC 9 cut(s) 28, 331, 1422, 1555, 1988, 2256, 2461, 2977, 3058
CspAI ACCGGT 1 cut(s) 916
CspCI CAANNNNNGTGG 2 cut(s) 1434, 1469
CviQI GTAC 9 cut(s) 28, 331, 1422, 1555, 1988, 2256, 2461, 2977, 3058
DinI GGCGCC 1 cut(s) 178
EaeI YGGCCR 2 cut(s) 3, 2901
EagI CGGCCG 1 cut(s) 2901
Ecl136II GAGCTC 3 cut(s) 612, 747, 901
EclXI CGGCCG 1 cut(s) 2901
Eco130I CCWWGG 4 cut(s) 731, 2639, 2756, 3110
Eco147I AGGCCT 1 cut(s) 2638
Eco24I GRGCYC 3 cut(s) 614, 749, 903
Eco31I GGTCTC 2 cut(s) 1693, 2691
Eco47I GGWCC 4 cut(s) 875, 1090, 1535, 2569
Eco52I CGGCCG 1 cut(s) 2901
Eco53kI GAGCTC 3 cut(s) 612, 747, 901
Eco57I CTGAAG 4 cut(s) 768, 1491, 1700, 2762
Eco81I CCTNAGG 2 cut(s) 879, 1893
Eco88I CYCGRG 1 cut(s) 1991
EcoICRI GAGCTC 3 cut(s) 612, 747, 901
EcoO109I RGGNCCY 3 cut(s) 875, 1090, 2261
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 1789, 2146, 2263
EcoT14I CCWWGG 4 cut(s) 731, 2639, 2756, 3110
EcoT38I GRGCYC 3 cut(s) 614, 749, 903
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 4 cut(s) 731, 2639, 2756, 3110
Esp3I CGTCTC 1 cut(s) 2285
FaqI GGGAC 2 cut(s) 138, 888
FauI CCCGC 1 cut(s) 2070
FbaI TGATCA 2 cut(s) 571, 1026
FblI GTMKAC 3 cut(s) 1278, 2357, 2721
Fnu4HI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1443, 1854, 2429, 3126
FriOI GRGCYC 3 cut(s) 614, 749, 903
Fsp4HI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1443, 1854, 2429, 3126
GlaI GCGC 8 cut(s) 178, 1023, 1037, 2427, 2798, 2800, 3065, 3152
GluI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1443, 1854, 2429, 3126
GsaI CCCAGC 3 cut(s) 2186, 2244, 2253
GsuI CTGGAG 1 cut(s) 2552
HaeII RGCGCY 2 cut(s) 180, 1039
HaeIII GGCC 5 cut(s) 5, 730, 2262, 2638, 2903
HapII CCGG 9 cut(s) 339, 347, 917, 962, 1392, 2066, 2656, 2865, 2904
HgaI GACGC 2 cut(s) 3146, 3200
HhaI GCGC 8 cut(s) 179, 1024, 1038, 2428, 2799, 2801, 3066, 3153
Hin1I GRCGYC 3 cut(s) 177, 3211, 3223
Hin6I GCGC 8 cut(s) 177, 1022, 1036, 2426, 2797, 2799, 3064, 3151
HinP1I GCGC 8 cut(s) 177, 1022, 1036, 2426, 2797, 2799, 3064, 3151
HincII GTYRAC 1 cut(s) 3161
HindII GTYRAC 1 cut(s) 3161
HinfI GANTC 9 cut(s) 550, 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
HpaII CCGG 9 cut(s) 339, 347, 917, 962, 1392, 2066, 2656, 2865, 2904
HphI GGTGA 7 cut(s) 353, 505, 976, 1142, 1352, 1910, 3082
Hpy166II GTNNAC 9 cut(s) 145, 1279, 1424, 1816, 2358, 2569, 2722, 2977, 3161
Hpy8I GTNNAC 9 cut(s) 145, 1279, 1424, 1816, 2358, 2569, 2722, 2977, 3161
HpyCH4III ACNGT 7 cut(s) 774, 1377, 1546, 1867, 2279, 2362, 2460
HpyCH4IV ACGT 2 cut(s) 141, 3166
HpyF10VI GCNNNNNNNGC 6 cut(s) 233, 2246, 2251, 2375, 2432, 2900
HpySE526I ACGT 2 cut(s) 141, 3166
Hsp92I GRCGYC 3 cut(s) 177, 3211, 3223
HspAI GCGC 8 cut(s) 177, 1022, 1036, 2426, 2797, 2799, 3064, 3151
KasI GGCGCC 1 cut(s) 176
KroI GCCGGC 1 cut(s) 2903
KroNI GCCGGC 1 cut(s) 2905
Ksp22I TGATCA 2 cut(s) 571, 1026
LguI GCTCTTC 5 cut(s) 602, 879, 1259, 1784, 2028
LmnI GCTCC 8 cut(s) 241, 752, 1033, 1255, 1828, 2471, 2533, 2758
Lsp1109I GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 3112
MaeII ACGT 2 cut(s) 141, 3166
MaeIII GTNAC 6 cut(s) 990, 1394, 1720, 1898, 2273, 3070
MfeI CAATTG 1 cut(s) 2118
MflI RGATCY 8 cut(s) 252, 969, 1111, 1875, 2416, 2589, 2932, 3130
MhlI GDGCHC 7 cut(s) 614, 749, 903, 936, 2023, 2476, 2998
MluCI AATT 9 cut(s) 149, 170, 334, 471, 2118, 2325, 2872, 2895, 3101
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 2903
MseI TTAA 1 cut(s) 975
MslI CAYNNNNRTG 3 cut(s) 848, 1659, 2606
MspA1I CMGCKG 1 cut(s) 1007
MspI CCGG 9 cut(s) 339, 347, 917, 962, 1392, 2066, 2656, 2865, 2904
MspR9I CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
MunI CAATTG 1 cut(s) 2118
Mva1269I GAATGC 1 cut(s) 2297
MvaI CCWGG 3 cut(s) 1791, 2148, 2265
MvnI CGCG 2 cut(s) 2799, 3066
MwoI GCNNNNNNNGC 6 cut(s) 233, 2246, 2251, 2375, 2432, 2900
NaeI GCCGGC 1 cut(s) 2905
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 2067, 2657
NcoI CCATGG 1 cut(s) 2756
NgoMIV GCCGGC 1 cut(s) 2903
NlaIV GGNNCC 7 cut(s) 178, 876, 1830, 2418, 2535, 2934, 3108
NmeAIII GCCGAG 2 cut(s) 1064, 2128
NmuCI GTSAC 3 cut(s) 990, 1898, 3070
NspI RCATGY 4 cut(s) 1186, 1777, 1947, 3064
NspV TTCGAA 1 cut(s) 380
PaeI GCATGC 1 cut(s) 1777
PagI TCATGA 2 cut(s) 1639, 2823
PasI CCCWGGG 2 cut(s) 1790, 2264
PauI GCGCGC 1 cut(s) 2797
PceI AGGCCT 1 cut(s) 2638
PciSI GCTCTTC 5 cut(s) 602, 879, 1259, 1784, 2028
PcsI WCGNNNNNNNCGW 2 cut(s) 458, 2242
PctI GAATGC 1 cut(s) 2297
PdiI GCCGGC 1 cut(s) 2905
PfeI GAWTC 8 cut(s) 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
PflMI CCANNNNNTGG 1 cut(s) 3207
PfoI TCCNGGA 1 cut(s) 2146
PinAI ACCGGT 1 cut(s) 916
PkrI GCNGC 9 cut(s) 81, 858, 1006, 1009, 1059, 1444, 1855, 2430, 3127
PleI GAGTC 1 cut(s) 558
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 558
PpuMI RGGWCCY 2 cut(s) 875, 1090
PsiI TTATAA 3 cut(s) 366, 2172, 2949
Psp124BI GAGCTC 3 cut(s) 614, 749, 903
Psp5II RGGWCCY 2 cut(s) 875, 1090
Psp6I CCWGG 3 cut(s) 1789, 2146, 2263
PspFI CCCAGC 3 cut(s) 2182, 2240, 2249
PspGI CCWGG 3 cut(s) 1789, 2146, 2263
PspN4I GGNNCC 7 cut(s) 178, 876, 1830, 2418, 2535, 2934, 3108
PspPI GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
PspPPI RGGWCCY 2 cut(s) 875, 1090
PstI CTGCAG 1 cut(s) 3130
PstNI CAGNNNCTG 2 cut(s) 1544, 2264
PsuI RGATCY 8 cut(s) 252, 969, 1111, 1875, 2416, 2589, 2932, 3130
PteI GCGCGC 1 cut(s) 2797
PvuII CAGCTG 1 cut(s) 1007
RsaI GTAC 9 cut(s) 29, 332, 1423, 1556, 1989, 2257, 2462, 2978, 3059
RsaNI GTAC 9 cut(s) 28, 331, 1422, 1555, 1988, 2256, 2461, 2977, 3058
RseI CAYNNNNRTG 3 cut(s) 848, 1659, 2606
SacI GAGCTC 3 cut(s) 614, 749, 903
SapI GCTCTTC 5 cut(s) 602, 879, 1259, 1784, 2028
SaqAI TTAA 1 cut(s) 975
SatI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1443, 1854, 2429, 3126
Sau96I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
SduI GDGCHC 7 cut(s) 614, 749, 903, 936, 2023, 2476, 2998
SfcI CTRYAG 1 cut(s) 3126
SfoI GGCGCC 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 380
SgrAI CRCCGGYG 1 cut(s) 961
SinI GGWCC 4 cut(s) 875, 1090, 1535, 2569
SmiMI CAYNNNNRTG 3 cut(s) 848, 1659, 2606
SmlI CTYRAG 1 cut(s) 2791
SmoI CTYRAG 1 cut(s) 2791
SpeI ACTAGT 1 cut(s) 674
SphI GCATGC 1 cut(s) 1777
Sse9I AATT 9 cut(s) 149, 170, 334, 471, 2118, 2325, 2872, 2895, 3101
SseBI AGGCCT 1 cut(s) 2638
SsiI CCGC 7 cut(s) 836, 1442, 1696, 1853, 2063, 2302, 2429
SspDI GGCGCC 1 cut(s) 176
SspI AATATT 1 cut(s) 2130
SstI GAGCTC 3 cut(s) 614, 749, 903
StuI AGGCCT 1 cut(s) 2638
StyD4I CCNGG 5 cut(s) 1789, 2065, 2146, 2263, 2655
StyI CCWWGG 4 cut(s) 731, 2639, 2756, 3110
TaaI ACNGT 7 cut(s) 774, 1377, 1546, 1867, 2279, 2362, 2460
TaiI ACGT 2 cut(s) 144, 3169
TasI AATT 9 cut(s) 149, 170, 334, 471, 2118, 2325, 2872, 2895, 3101
TatI WGTACW 6 cut(s) 330, 1421, 1554, 2460, 2976, 3057
TauI GCSGC 3 cut(s) 1445, 1856, 2431
TfiI GAWTC 8 cut(s) 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
Tru1I TTAA 1 cut(s) 975
Tru9I TTAA 1 cut(s) 975
TseFI GTSAC 3 cut(s) 990, 1898, 3070
TseI GCWGC 6 cut(s) 79, 856, 1004, 1007, 1057, 3125
Tsp45I GTSAC 3 cut(s) 990, 1898, 3070
TspGWI ACGGA 4 cut(s) 142, 191, 1092, 1673
Van91I CCANNNNNTGG 1 cut(s) 3207
VpaK11BI GGWCC 4 cut(s) 875, 1090, 1535, 2569
XapI RAATTY 2 cut(s) 149, 170
XceI RCATGY 4 cut(s) 1186, 1777, 1947, 3064
XcmI CCANNNNNNNNNTGG 1 cut(s) 3094
XmaJI CCTAGG 1 cut(s) 2639
XmiI GTMKAC 3 cut(s) 1278, 2357, 2721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.