Rw1G020490

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000671
Physical Location & Seq
Forward (+)
44146228 .. 44151546
5319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020490.1

Sequence Viewer

Length: 2982 bp
ATGGCTGCGATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCCGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTCGATGCCAAATTGAAGGAACATGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCACGCAATTCTTCCCCCAAGGCAAAGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGTTGGAGGCCAAGGAAAAGCGAAATGACAGAGAAGAATGGGGAGATGGGCGTGAAGAAGATAGGCCCAGAGGTCGAGATGGAGGTGGAGGTAGGGGTAGGGAAAGAGATACAAATCGAGACAGAGACAGAGACAGGGACAGAGACAGGGACAGGGACAGGGATAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAATCATTATCATGATTATGATAAGGGAAGGCATACGGATCGATCCAATAAGCACAGGAGGGATGAGTATGAAAAGGATGGAGATGTTAGAGAAGATGATGTTGATAGGAGAGGAAGTAGGGATCAGCAGAATGGATTGCACCATTCTGATGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCTCAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCTACTCGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTTAAGGTGATTTCAATTTCTGGTCAAAAGTTGAGCCTGTCAATGAGGGATGTTGATCAGCATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCGTCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCTGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTTTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTCACGCAGTATCTTGCAGAAGCTGGGTACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCAATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTGTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACGGATGGTATGCTTCTTAGGGAGATTCTGCTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATCCCCGGAAGAACTTTTCCTGTAGAAATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCGGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTATATGAGAGGATGAAGGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCAAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCTCTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAACAACGAGCTGGGCGTGCTGGCCGTACAGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTACTACATTAACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATTCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

993

Amino Acids

112.77

Weight (kDa)

5.49

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 238 - 305 3.4e-11 S1 RNA binding domain
DEAD PF00270 544 - 693 5e-08 DEAD/DEAH box helicase
AAA_22 PF13401 552 - 685 2.3e-06 AAA domain
Helicase_C PF00271 737 - 867 8.6e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 929 - 957 6.7e-11 Helicase associated domain (HA2), winged-helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 720, 2511
AasI GACNNNNNNGTC 1 cut(s) 994
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 1466
AciI CCGC 4 cut(s) 538, 2035, 2617, 2641
AcoI YGGCCR 5 cut(s) 1085, 2565, 2590, 2856, 2898
AcsI RAATTY 3 cut(s) 152, 2214, 2673
AcuI CTGAAG 3 cut(s) 1646, 1830, 2646
AfaI GTAC 7 cut(s) 38, 208, 1736, 1762, 1895, 1952, 2596
AfiI CCNNNNNNNGG 6 cut(s) 46, 1466, 2406, 2463, 2521, 2522
AhlI ACTAGT 1 cut(s) 2536
AjnI CCWGG 5 cut(s) 1277, 1704, 2404, 2485, 2602
AjuI GAANNNNNNNTTGG 4 cut(s) 776, 808, 1619, 1651
Alw21I GWGCWC 1 cut(s) 1106
Alw26I GTCTC 9 cut(s) 438, 444, 450, 462, 736, 1040, 1457, 1676, 2032
AlwNI CAGNNNCTG 3 cut(s) 1466, 1730, 2603
Ama87I CYCGRG 2 cut(s) 32, 1141
ApeKI GCWGC 7 cut(s) 5, 1343, 1346, 1393, 1396, 1781, 2167
ApoI RAATTY 3 cut(s) 152, 2214, 2673
ArsI GACNNNNNNTTYG 4 cut(s) 2031, 2063, 2474, 2506
Asp700I GAANNNNTTC 1 cut(s) 1638
AspS9I GGNCC 5 cut(s) 390, 1016, 1874, 2600, 2908
AsuC2I CCSGG 1 cut(s) 2130
AsuHPI GGTGA 8 cut(s) 859, 889, 1098, 1290, 1481, 1691, 2249, 2757
AvaI CYCGRG 2 cut(s) 32, 1141
AvaII GGWCC 2 cut(s) 1874, 2908
BaeGI GKGCMC 1 cut(s) 2362
BalI TGGCCA 1 cut(s) 2900
BamHI GGATCC 2 cut(s) 2755, 2872
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 1106, 2815
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1559, 2822
Bbv12I GWGCWC 1 cut(s) 1106
BbvCI CCTCAGC 1 cut(s) 1218
BbvI GCAGC 6 cut(s) 1333, 1355, 1405, 1408, 1768, 2179
BceAI ACGGC 1 cut(s) 2577
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 5 cut(s) 1279, 1706, 2406, 2487, 2604
BciVI GTATCC 1 cut(s) 1176
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 1 cut(s) 2130
BcoDI GTCTC 9 cut(s) 438, 444, 450, 462, 736, 1040, 1457, 1676, 2032
BcuI ACTAGT 1 cut(s) 2536
BfaI CTAG 6 cut(s) 83, 104, 2528, 2537, 2816, 2980
BfmI CTRYAG 3 cut(s) 1287, 1791, 2145
BfuI GTATCC 1 cut(s) 1176
BisI GCNGC 7 cut(s) 6, 1344, 1347, 1394, 1397, 1782, 2168
BlsI GCNGC 7 cut(s) 7, 1345, 1348, 1395, 1398, 1783, 2169
BmcAI AGTACT 1 cut(s) 1952
Bme1390I CCNGG 6 cut(s) 1279, 1706, 2130, 2406, 2487, 2604
Bme18I GGWCC 2 cut(s) 1874, 2908
BmeT110I CYCGRG 2 cut(s) 32, 1141
BmgT120I GGNCC 5 cut(s) 390, 1016, 1874, 2600, 2908
BmiI GGNNCC 5 cut(s) 181, 2229, 2757, 2812, 2874
BmrFI CCNGG 6 cut(s) 1279, 1706, 2130, 2406, 2487, 2604
BmrI ACTGGG 2 cut(s) 945, 2361
BmuI ACTGGG 2 cut(s) 945, 2361
BpiI GAAGAC 2 cut(s) 1559, 2822
Bpu10I CCTNAGC 1 cut(s) 1218
BpuEI CTTGAG 1 cut(s) 1250
BpuMI CCSGG 1 cut(s) 2130
Bsa29I ATCGAT 2 cut(s) 586, 2460
BsaAI YACGTR 1 cut(s) 1772
BsaBI GATNNNNATC 3 cut(s) 438, 924, 1434
BsaI GGTCTC 3 cut(s) 736, 1040, 2032
BsaWI WCCGGW 1 cut(s) 1108
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 1466, 2406, 2463, 2521, 2522
Bse118I RCCGGY 1 cut(s) 1083
Bse1I ACTGG 8 cut(s) 940, 1471, 1690, 1876, 2287, 2348, 2367, 2901
Bse3DI GCAATG 4 cut(s) 1791, 2650, 2724, 2941
Bse8I GATNNNNATC 3 cut(s) 438, 924, 1434
BseBI CCWGG 5 cut(s) 1279, 1706, 2406, 2487, 2604
BseCI ATCGAT 2 cut(s) 586, 2460
BseJI GATNNNNATC 3 cut(s) 438, 924, 1434
BseLI CCNNNNNNNGG 6 cut(s) 46, 1466, 2406, 2463, 2521, 2522
BseMI GCAATG 4 cut(s) 1791, 2650, 2724, 2941
BseMII CTCAG 8 cut(s) 111, 981, 1209, 1518, 1961, 2073, 2322, 2619
BseNI ACTGG 8 cut(s) 940, 1471, 1690, 1876, 2287, 2348, 2367, 2901
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 2362
BseXI GCAGC 6 cut(s) 1333, 1355, 1405, 1408, 1768, 2179
BseYI CCCAGC 2 cut(s) 1730, 2579
BsgI GTGCAG 3 cut(s) 1332, 1344, 1362
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 2 cut(s) 586, 2460
BsiHKAI GWGCWC 1 cut(s) 1106
BsiHKCI CYCGRG 2 cut(s) 32, 1141
BsiSI CCGG 4 cut(s) 1084, 1109, 2130, 2231
BslFI GGGAC 8 cut(s) 107, 141, 476, 488, 494, 2274, 2638, 2707
BslI CCNNNNNNNGG 6 cut(s) 46, 1466, 2406, 2463, 2521, 2522
BsmAI GTCTC 9 cut(s) 438, 444, 450, 462, 736, 1040, 1457, 1676, 2032
BsmFI GGGAC 8 cut(s) 107, 141, 476, 488, 494, 2274, 2638, 2707
Bso31I GGTCTC 3 cut(s) 736, 1040, 2032
BsoBI CYCGRG 2 cut(s) 32, 1141
Bsp1286I GDGCHC 3 cut(s) 1106, 2362, 2815
Bsp1407I TGTACA 1 cut(s) 1760
BspACI CCGC 4 cut(s) 538, 2035, 2617, 2641
BspCNI CTCAG 8 cut(s) 112, 980, 1210, 1519, 1960, 2074, 2323, 2620
BspDI ATCGAT 2 cut(s) 586, 2460
BspHI TCATGA 2 cut(s) 556, 1978
BspLI GGNNCC 5 cut(s) 181, 2229, 2757, 2812, 2874
BspMAI CTGCAG 1 cut(s) 1291
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 736, 1040, 2032
BsrDI GCAATG 4 cut(s) 1791, 2650, 2724, 2941
BsrFI RCCGGY 1 cut(s) 1083
BsrGI TGTACA 1 cut(s) 1760
BsrI ACTGG 8 cut(s) 940, 1471, 1690, 1876, 2287, 2348, 2367, 2901
BssAI RCCGGY 1 cut(s) 1083
BssT1I CCWWGG 7 cut(s) 45, 54, 242, 336, 1442, 2073, 2911
Bst2UI CCWGG 5 cut(s) 1279, 1706, 2406, 2487, 2604
Bst4CI ACNGT 7 cut(s) 137, 1131, 1558, 1885, 2111, 2206, 2804
Bst6I CTCTTC 7 cut(s) 845, 1053, 1088, 1203, 1806, 2405, 2817
BstAPI GCANNNNNTGC 1 cut(s) 1609
BstAUI TGTACA 1 cut(s) 1760
BstBAI YACGTR 1 cut(s) 1772
BstC8I GCNNGC 7 cut(s) 1085, 1257, 1610, 2284, 2586, 2590, 2902
BstMAI GTCTC 9 cut(s) 438, 444, 450, 462, 736, 1040, 1457, 1676, 2032
BstMWI GCNNNNNNNGC 3 cut(s) 1393, 1609, 2585
BstNI CCWGG 5 cut(s) 1279, 1706, 2406, 2487, 2604
BstNSI RCATGY 2 cut(s) 1525, 2286
BstSCI CCNGG 6 cut(s) 1277, 1704, 2128, 2404, 2485, 2602
BstSFI CTRYAG 3 cut(s) 1287, 1791, 2145
BstSLI GKGCMC 1 cut(s) 2362
BstV1I GCAGC 6 cut(s) 1333, 1355, 1405, 1408, 1768, 2179
BstV2I GAAGAC 2 cut(s) 1559, 2822
BstX2I RGATCY 4 cut(s) 1429, 1450, 2755, 2872
BstYI RGATCY 4 cut(s) 1429, 1450, 2755, 2872
Bsu15I ATCGAT 2 cut(s) 586, 2460
BsuI GTATCC 1 cut(s) 1176
BsuTUI ATCGAT 2 cut(s) 586, 2460
BtsI GCAGTG 2 cut(s) 1653, 2926
BtsIMutI CAGTG 6 cut(s) 1653, 1869, 2202, 2374, 2625, 2926
Cac8I GCNNGC 7 cut(s) 1085, 1257, 1610, 2284, 2586, 2590, 2902
CaiI CAGNNNCTG 3 cut(s) 1466, 1730, 2603
CciI TCATGA 2 cut(s) 556, 1978
Cfr10I RCCGGY 1 cut(s) 1083
Cfr13I GGNCC 5 cut(s) 390, 1016, 1874, 2600, 2908
ClaI ATCGAT 2 cut(s) 586, 2460
CsiI ACCWGGT 1 cut(s) 1277
Csp6I GTAC 7 cut(s) 37, 207, 1735, 1761, 1894, 1951, 2595
CviQI GTAC 7 cut(s) 37, 207, 1735, 1761, 1894, 1951, 2595
DrdI GACNNNNNNGTC 1 cut(s) 994
DseDI GACNNNNNNGTC 1 cut(s) 994
EaeI YGGCCR 5 cut(s) 1085, 2565, 2590, 2856, 2898
Eam1104I CTCTTC 7 cut(s) 845, 1053, 1088, 1203, 1806, 2405, 2817
EarI CTCTTC 7 cut(s) 845, 1053, 1088, 1203, 1806, 2405, 2817
EciI GGCGGA 1 cut(s) 553
Ecl136II GAGCTC 1 cut(s) 1104
Eco130I CCWWGG 7 cut(s) 45, 54, 242, 336, 1442, 2073, 2911
Eco147I AGGCCT 1 cut(s) 2978
Eco24I GRGCYC 2 cut(s) 1106, 2815
Eco31I GGTCTC 3 cut(s) 736, 1040, 2032
Eco32I GATATC 2 cut(s) 310, 1002
Eco47I GGWCC 2 cut(s) 1874, 2908
Eco53kI GAGCTC 1 cut(s) 1104
Eco57I CTGAAG 3 cut(s) 1646, 1830, 2646
Eco88I CYCGRG 2 cut(s) 32, 1141
EcoICRI GAGCTC 1 cut(s) 1104
EcoO109I RGGNCCY 1 cut(s) 2600
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 5 cut(s) 1277, 1704, 2404, 2485, 2602
EcoRV GATATC 2 cut(s) 310, 1002
EcoT14I CCWWGG 7 cut(s) 45, 54, 242, 336, 1442, 2073, 2911
EcoT22I ATGCAT 1 cut(s) 2194
EcoT38I GRGCYC 2 cut(s) 1106, 2815
ErhI CCWWGG 7 cut(s) 45, 54, 242, 336, 1442, 2073, 2911
FalI AAGNNNNNCTT 2 cut(s) 2814, 2846
FaqI GGGAC 8 cut(s) 107, 141, 476, 488, 494, 2274, 2638, 2707
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 7 cut(s) 6, 1344, 1347, 1394, 1397, 1782, 2168
FriOI GRGCYC 2 cut(s) 1106, 2815
Fsp4HI GCNGC 7 cut(s) 6, 1344, 1347, 1394, 1397, 1782, 2168
FspBI CTAG 6 cut(s) 83, 104, 2528, 2537, 2816, 2980
GluI GCNGC 7 cut(s) 6, 1344, 1347, 1394, 1397, 1782, 2168
GsaI CCCAGC 2 cut(s) 1734, 2583
HapII CCGG 4 cut(s) 1084, 1109, 2130, 2231
HindIII AAGCTT 1 cut(s) 1580
HinfI GANTC 9 cut(s) 977, 1318, 1336, 1415, 1435, 1924, 2531, 2929, 2951
HpaII CCGG 4 cut(s) 1084, 1109, 2130, 2231
HphI GGTGA 8 cut(s) 859, 889, 1098, 1290, 1481, 1691, 2249, 2757
Hpy166II GTNNAC 5 cut(s) 209, 1737, 1761, 1763, 2908
Hpy188I TCNGA 8 cut(s) 67, 694, 970, 1434, 2454, 2679, 2835, 2956
Hpy8I GTNNAC 5 cut(s) 209, 1737, 1761, 1763, 2908
Hpy99I CGWCG 1 cut(s) 1000
HpyCH4III ACNGT 7 cut(s) 137, 1131, 1558, 1885, 2111, 2206, 2804
HpyCH4IV ACGT 1 cut(s) 1771
HpyF10VI GCNNNNNNNGC 3 cut(s) 1393, 1609, 2585
HpySE526I ACGT 1 cut(s) 1771
KroI GCCGGC 1 cut(s) 1083
KroNI GCCGGC 1 cut(s) 1085
Ksp22I TGATCA 1 cut(s) 925
LmnI GCTCC 4 cut(s) 1214, 1372, 2227, 2810
Lsp1109I GCAGC 6 cut(s) 1333, 1355, 1405, 1408, 1768, 2179
MabI ACCWGGT 1 cut(s) 1277
MaeI CTAG 6 cut(s) 83, 104, 2528, 2537, 2816, 2980
MaeII ACGT 1 cut(s) 1771
MflI RGATCY 4 cut(s) 1429, 1450, 2755, 2872
MhlI GDGCHC 3 cut(s) 1106, 2362, 2815
MlsI TGGCCA 1 cut(s) 2900
MluNI TGGCCA 1 cut(s) 2900
MlyI GAGTC 1 cut(s) 1345
MmeI TCCRAC 3 cut(s) 309, 1392, 1822
Mox20I TGGCCA 1 cut(s) 2900
Mph1103I ATGCAT 1 cut(s) 2194
MroNI GCCGGC 1 cut(s) 1083
MroXI GAANNNNTTC 1 cut(s) 1638
MscI TGGCCA 1 cut(s) 2900
MseI TTAA 7 cut(s) 873, 1242, 1314, 1368, 2198, 2222, 2705
MslI CAYNNNNRTG 6 cut(s) 555, 561, 693, 1742, 1998, 2946
Msp20I TGGCCA 1 cut(s) 2900
MspA1I CMGCKG 1 cut(s) 1346
MspI CCGG 4 cut(s) 1084, 1109, 2130, 2231
MspR9I CCNGG 6 cut(s) 1279, 1706, 2130, 2406, 2487, 2604
MvaI CCWGG 5 cut(s) 1279, 1706, 2406, 2487, 2604
MwoI GCNNNNNNNGC 3 cut(s) 1393, 1609, 2585
NaeI GCCGGC 1 cut(s) 1085
NciI CCSGG 1 cut(s) 2130
NgoMIV GCCGGC 1 cut(s) 1083
NlaIV GGNNCC 5 cut(s) 181, 2229, 2757, 2812, 2874
NmeAIII GCCGAG 5 cut(s) 133, 208, 799, 2467, 2884
NmuCI GTSAC 7 cut(s) 1021, 1296, 1558, 1708, 2059, 2237, 2289
NsiI ATGCAT 1 cut(s) 2194
NspI RCATGY 2 cut(s) 1525, 2286
PaeI GCATGC 1 cut(s) 2286
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 556, 1978
PceI AGGCCT 1 cut(s) 2978
PcsI WCGNNNNNNNCGW 1 cut(s) 2581
PdiI GCCGGC 1 cut(s) 1085
PdmI GAANNNNTTC 1 cut(s) 1638
PfeI GAWTC 8 cut(s) 977, 1318, 1415, 1435, 1924, 2531, 2929, 2951
PflMI CCANNNNNTGG 1 cut(s) 1466
PkrI GCNGC 7 cut(s) 7, 1345, 1348, 1395, 1398, 1783, 2169
PleI GAGTC 1 cut(s) 1344
PpsI GAGTC 1 cut(s) 1344
Ppu21I YACGTR 1 cut(s) 1772
PsiI TTATAA 2 cut(s) 720, 2511
Psp124BI GAGCTC 1 cut(s) 1106
Psp6I CCWGG 5 cut(s) 1277, 1704, 2404, 2485, 2602
PspFI CCCAGC 2 cut(s) 1730, 2579
PspGI CCWGG 5 cut(s) 1277, 1704, 2404, 2485, 2602
PspN4I GGNNCC 5 cut(s) 181, 2229, 2757, 2812, 2874
PspPI GGNCC 5 cut(s) 390, 1016, 1874, 2600, 2908
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 1291
PstNI CAGNNNCTG 3 cut(s) 1466, 1730, 2603
PsuI RGATCY 4 cut(s) 1429, 1450, 2755, 2872
PvuII CAGCTG 1 cut(s) 1346
RsaI GTAC 7 cut(s) 38, 208, 1736, 1762, 1895, 1952, 2596
RsaNI GTAC 7 cut(s) 37, 207, 1735, 1761, 1894, 1951, 2595
RseI CAYNNNNRTG 6 cut(s) 555, 561, 693, 1742, 1998, 2946
SacI GAGCTC 1 cut(s) 1106
SaqAI TTAA 7 cut(s) 873, 1242, 1314, 1368, 2198, 2222, 2705
SatI GCNGC 7 cut(s) 6, 1344, 1347, 1394, 1397, 1782, 2168
Sau96I GGNCC 5 cut(s) 390, 1016, 1874, 2600, 2908
ScaI AGTACT 1 cut(s) 1952
SchI GAGTC 1 cut(s) 1345
ScrFI CCNGG 6 cut(s) 1279, 1706, 2130, 2406, 2487, 2604
SduI GDGCHC 3 cut(s) 1106, 2362, 2815
SexAI ACCWGGT 1 cut(s) 1277
SfcI CTRYAG 3 cut(s) 1287, 1791, 2145
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 2 cut(s) 1874, 2908
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 6 cut(s) 555, 561, 693, 1742, 1998, 2946
SmlI CTYRAG 2 cut(s) 32, 1229
SmoI CTYRAG 2 cut(s) 32, 1229
SpeI ACTAGT 1 cut(s) 2536
SphI GCATGC 1 cut(s) 2286
SseBI AGGCCT 1 cut(s) 2978
SsiI CCGC 4 cut(s) 538, 2035, 2617, 2641
SspI AATATT 1 cut(s) 1311
SspMI CTAG 6 cut(s) 83, 104, 2528, 2537, 2816, 2980
SstI GAGCTC 1 cut(s) 1106
StuI AGGCCT 1 cut(s) 2978
StyD4I CCNGG 6 cut(s) 1277, 1704, 2128, 2404, 2485, 2602
StyI CCWWGG 7 cut(s) 45, 54, 242, 336, 1442, 2073, 2911
TaaI ACNGT 7 cut(s) 137, 1131, 1558, 1885, 2111, 2206, 2804
TaiI ACGT 1 cut(s) 1774
TaqI TCGA 8 cut(s) 33, 156, 400, 442, 586, 998, 2106, 2460
TatI WGTACW 3 cut(s) 1760, 1893, 1950
TfiI GAWTC 8 cut(s) 977, 1318, 1415, 1435, 1924, 2531, 2929, 2951
Tru1I TTAA 7 cut(s) 873, 1242, 1314, 1368, 2198, 2222, 2705
Tru9I TTAA 7 cut(s) 873, 1242, 1314, 1368, 2198, 2222, 2705
TscAI CASTG 6 cut(s) 1653, 1876, 2209, 2374, 2632, 2926
TseFI GTSAC 7 cut(s) 1021, 1296, 1558, 1708, 2059, 2237, 2289
TseI GCWGC 7 cut(s) 5, 1343, 1346, 1393, 1396, 1781, 2167
Tsp45I GTSAC 7 cut(s) 1021, 1296, 1558, 1708, 2059, 2237, 2289
TspGWI ACGGA 4 cut(s) 194, 596, 1916, 2240
TspRI CASTG 6 cut(s) 1653, 1876, 2209, 2374, 2632, 2926
Van91I CCANNNNNTGG 1 cut(s) 1466
VpaK11BI GGWCC 2 cut(s) 1874, 2908
XapI RAATTY 3 cut(s) 152, 2214, 2673
XceI RCATGY 2 cut(s) 1525, 2286
XhoI CTCGAG 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 1638
XspI CTAG 6 cut(s) 83, 104, 2528, 2537, 2816, 2980
ZrmI AGTACT 1 cut(s) 1952
Zsp2I ATGCAT 1 cut(s) 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.