pycom16g21640

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000671
Physical Location & Seq
Reverse (-)
20321510 .. 20324590
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g21640.3

Sequence Viewer

Length: 2952 bp
ATGGGGAAATCGACAAGCACATCAATTTTTTTTTTTGGGAAAAATAAAAGCTGGGCGCTCCAGGTCAACCGACCTCTTATGCCTGTTTCCATGGAAGAAGGGTCAGCGTTCAACAAGCTCCAATACCTTTCGCTGGTCTCCAACATTTGTTCTGAACTCGAAGCTAACCTCGGCATATCCGACAGAGTCTTGGCCGAGTTCATAGCCGAGACAGGTCGCAACTCCGACGTTGTCGACGACTTCCAGGCAACCTTAAAAGAAAAGGGTGCGGACATGCCGGAATATCTCAGCCGTTCCTTGTTGAAGATCATCCACGCTATTCTTCCTCCAAAGGGAAACCCCTCTCACTCTCACAAGAACAAGAACCCTGCCGCCATTGAGGAAGGACTTGAAGGGATTCAGAGGAGCAAGAGGCAGAGGGATGACGATGACGATGTTAGGATCGGGTACCGATGTAGAAGGAGGTATTCGGATTCGGAAGAGGATGAGCAGGAGAGATGGGAGATGAAGCAGCTGATTGCCTCCGGCGTTTCGGGTGCTGCAATGCGTGATGGACACGAATTGCAATTTCGAGAAGAGGGTTTTGGGGAAGAAGAGCATGAGACCGAGTTTGATGAGGAGCAATTACTACTTTTGCAAGGGCAGAGTGGATACTCGATTGATATGTCACCGGTTAGGATTGTGCCGAATCCCAACGGGTCATTGAGTCATGCAGCAGCTCTTCGATCAGCGCTGCAAAAGGAGAGTAGAGAAATAACAAGAGTGGAAGAGAACATGAGGCTCAATGCAGCTCCAAAGGATTTGAATCGCGCTTGGGAAGACCCTATGCCGGGAAGGGGTGAGAGGCATTTTGCACAAGAGCTTCTGGGTGCTACTGCTGCTCGCTTTTCCGGTTATGACAGACACGAGCGGAACAAGCGTGCTTACGAAGGAGCCCTGGATCTTGGGCAGAGTTCAAGCCTTTCTCTTTGCGAACAGCGCCAGAGCTTACCAGTTTACAGGTTGAAGAAAGAACTGATTCAGGCTGTGAAAGCAAATCGGGTTCTTGTTGTCATTGGTGAGACTGGTTCGGGTAAAACTACGCAGATAACTCAATATCTTGCTGAAGCAGGTTACACCACAAGGGGAAAAATCGGTTGCACTCAACCGCGAAGAGTTGCAGCCACGTCTGTGGCCAAGAGAGTTGCTGAGGAATTTGGTTGTCGATTAGGGGAGGAAGTTGGGTACTCTGTACGATTTGATGATTGCACTGGACCGGATACTGTGATCAAGTACATGACCGAGGGGATACTTTTAAGGGAAATTTTGAGTGATAAAAACCTGTCTCAGTACTCCGTGATCATCCTTGATGAAGCTCATGAAAGAACAGTCTCCACTGACGTTCTTTTCGGATTGCTGAAGCAACTTGTTGAAAGGCGACCTGATCTTCGCTTGATTGTTACCTCTGCCACATTGGATGCAGTGAAGTTTTCAGATTATTTCTTTCATTGTCAGATATTTACGATTCCTGGTAGAACTTATCCGGTAGAATTAATGTACTCGAAACAGCCAGAAAGTGATTACTTGGATGCAGCTATATTGACTGTTCTGCAACTTCACTTGACTAAACCCGAAGGTGACATACTTATGTTCCTGACGGGTGAGGAGGAGATTCAATTTGCTTGTCAGTCTTTGTATGAAAGGATGAAAGTACTCGGTAGAGAAGCTCCAGAATTGATTATTCTACCAGCTTATGGTGCCCAACCGAGTGCAGAGCAATCGAAGATATTCAAGCCTGCTCCTTCAGGGAAGAGAAAAGTGGTTGTTGCTACCAATATTGCTGAAGCCTCTTTGACCATTGATGGGATATACTACGTAATTGATCCGGGATTTGCAAAGCAAAATGTATTTAACCCAAAGCTTGGGATTGATTTACTGGTTGTAACTCCAATTTCTCAAGCATCAGCCAAACAACGAGCTGGGCGCGCTGGGCGGACAGGACCAGGGGAATGTTACCGCCTTTACACTGAGAGCGCTTTTCACAATGAGATGTCTCCTACCACAGTTCCAGAAATCCTAAGAACAAATCTTGTGCATACTACACTTATGATGAAAGCAATGGGGATACACGATCTCAAATCTTTTGATTTTATGGATCCACCGTCATCCCAAGCGCTTGAGTCTGCCATGAAACAGCTGCAAAGTCTAGGAGCTTTGGATGAGGAGGGGTTACTGACCAAATTGGGTAGTAGAATGGCATTGTTGCCTCTGGATCCACCTTTGTCGAAAATGCTGCTTGCCAGTGCGAACCTTGGATGCAGTGATGAGATTGTGACAATCATTGCCATGCTTCAGGCGGGAAATATCTTCTACAGGCCTAGAGAAAAACAAAGCCAAGCAGATCAAAAGAAGGCCAAGTTTTTCCAGCCTGAGGGAGACCATCTGACTTTACTCACTGTTTACGAGGCTTGGAAAGCCAAGGATTTTTCTAAACCCTGGTGTTCTGAGAACTTTTTACAGTTTCGGTCATTGAGCAGGGCAAGGGATATCAGGAAACAGCTTCTTACTGTCATGGACAAGTATGAATTAGAGGTTGTAAGTGCAGGAAGAAATGTCGTAATGGTCAGAAAGGCAATAATTGCTGGATGCTTCTTCAGAGTGGCAAGAAAGGATCCAAAGGAGGGTTATAGAACCCTAGTCGATAATCAGCAAGTTTATATCCACCCGAGCAGCGTTCTTTTCCAGAGACAACCAGCTTGGGTCATCTACAATGAGCTTGTGATGACCACGAAGGAGTACATGCGTGAGGTCAGTGCCATAGAACCCAAGTGGCTGATGGAGCAGGTTCCAAGATTCTTTAAGGCGGCAAATCCTACTAAGATGAGCAACGGCAAAAATCAAGAACGTACTGAACCACCGTATGACAGATATCATGAGCCCAACTCATGGCGCCTGAGCAGAAGGCGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

984

Amino Acids

110.87

Weight (kDa)

6.83

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1100, 2812
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 3 cut(s) 447, 1736, 2928
AccB7I CCANNNNNTGG 3 cut(s) 1733, 1901, 2925
AccBSI CCGCTC 1 cut(s) 910
AccI GTMKAC 1 cut(s) 234
AccII CGCG 3 cut(s) 810, 1150, 1965
AciI CCGC 8 cut(s) 269, 372, 910, 1148, 1972, 1996, 2336, 2843
AclWI GGATC 9 cut(s) 449, 948, 1856, 2129, 2142, 2246, 2259, 2645, 2658
AcoI YGGCCR 2 cut(s) 192, 1173
AcsI RAATTY 2 cut(s) 1193, 1302
AcuI CTGAAG 6 cut(s) 1125, 1418, 1767, 1842, 2315, 2617
AcyI GRCGYC 1 cut(s) 2929
AfaI GTAC 9 cut(s) 449, 1226, 1233, 1274, 1331, 1538, 1692, 2777, 2887
AfeI AGCGCT 3 cut(s) 732, 2014, 2154
AgeI ACCGGT 1 cut(s) 670
AgsI TTSAA 9 cut(s) 112, 304, 392, 805, 957, 1006, 1412, 1655, 1771
AjiI CACGTC 1 cut(s) 1167
AjnI CCWGG 6 cut(s) 60, 243, 936, 1507, 1981, 2474
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AleI CACNNNNGTG 1 cut(s) 1169
Alw26I GTCTC 9 cut(s) 142, 203, 596, 1055, 1329, 1375, 2037, 2409, 2719
AlwI GGATC 9 cut(s) 449, 948, 1856, 2129, 2142, 2246, 2259, 2645, 2658
Ama87I CYCGRG 1 cut(s) 2704
Aor51HI AGCGCT 3 cut(s) 732, 2014, 2154
AoxI GGCC 4 cut(s) 192, 1173, 2354, 2391
ApoI RAATTY 2 cut(s) 1193, 1302
ArsI GACNNNNNNTTYG 4 cut(s) 1370, 1402, 1410, 1442
AseI ATTAAT 1 cut(s) 1532
AsiGI ACCGGT 1 cut(s) 670
Asp700I GAANNNNTTC 3 cut(s) 396, 1017, 1766
Asp718I GGTACC 1 cut(s) 447
AspLEI GCGC 9 cut(s) 58, 733, 812, 981, 1965, 1967, 2015, 2155, 2931
AspS9I GGNCC 2 cut(s) 1253, 1979
AsuC2I CCSGG 2 cut(s) 831, 1866
AsuHPI GGTGA 5 cut(s) 660, 851, 1070, 1628, 1652
AvaI CYCGRG 1 cut(s) 2704
AvaII GGWCC 2 cut(s) 1253, 1979
AxyI CCTNAGG 1 cut(s) 2409
BaeGI GKGCMC 1 cut(s) 1741
BaeI ACNNNNGTAYC 2 cut(s) 439, 472
BalI TGGCCA 1 cut(s) 1175
BamHI GGATCC 3 cut(s) 2134, 2251, 2650
BanI GGYRCC 3 cut(s) 447, 1736, 2928
BanII GRGCYC 2 cut(s) 937, 2919
BarI GAAGNNNNNNTAC 2 cut(s) 1605, 1637
BauI CACGAG 1 cut(s) 905
BbsI GAAGAC 1 cut(s) 825
BbvCI CCTCAGC 1 cut(s) 1188
BccI CCATC 5 cut(s) 492, 545, 1835, 2427, 2809
BceAI ACGGC 2 cut(s) 276, 2884
BcgI CGANNNNNNTGC 4 cut(s) 1740, 1774, 2254, 2288
BciT130I CCWGG 6 cut(s) 62, 245, 938, 1509, 1983, 2476
BciVI GTATCC 4 cut(s) 644, 1252, 1281, 2097
BclI TGATCA 2 cut(s) 1266, 1338
BcnI CCSGG 2 cut(s) 831, 1866
BcoDI GTCTC 9 cut(s) 142, 203, 596, 1055, 1329, 1375, 2037, 2409, 2719
BfaI CTAG 3 cut(s) 2186, 2358, 2675
BfmI CTRYAG 1 cut(s) 2350
BfoI RGCGCY 6 cut(s) 59, 734, 982, 2016, 2156, 2932
BfuAI ACCTGC 2 cut(s) 1100, 2812
BfuI GTATCC 4 cut(s) 644, 1252, 1281, 2097
BmcAI AGTACT 2 cut(s) 1331, 1692
Bme1390I CCNGG 8 cut(s) 62, 245, 831, 938, 1509, 1866, 1983, 2476
Bme18I GGWCC 2 cut(s) 1253, 1979
BmeT110I CYCGRG 1 cut(s) 2704
BmgBI CACGTC 1 cut(s) 1167
BmgT120I GGNCC 2 cut(s) 1253, 1979
BmiI GGNNCC 8 cut(s) 449, 934, 1738, 2136, 2253, 2652, 2826, 2930
BmrFI CCNGG 8 cut(s) 62, 245, 831, 938, 1509, 1866, 1983, 2476
BmsI GCATC 5 cut(s) 1447, 1558, 1949, 2285, 2615
BpiI GAAGAC 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 2906, 2938
BpmI CTGGAG 2 cut(s) 44, 1692
Bpu10I CCTNAGC 2 cut(s) 1188, 2933
BpuEI CTTGAG 2 cut(s) 1920, 2177
BpuMI CCSGG 2 cut(s) 831, 1866
BsaAI YACGTR 1 cut(s) 1855
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 2929
BsaI GGTCTC 3 cut(s) 142, 596, 2409
BsaJI CCNNGG 8 cut(s) 90, 169, 936, 1281, 1982, 2290, 2457, 2474
BsaWI WCCGGW 4 cut(s) 670, 890, 1255, 1522
Bse118I RCCGGY 1 cut(s) 670
Bse1I ACTGG 5 cut(s) 992, 1069, 1255, 1920, 2280
Bse21I CCTNAGG 1 cut(s) 2409
Bse3DI GCAATG 3 cut(s) 549, 2103, 2319
Bse8I GATNNNNATC 1 cut(s) 804
BseBI CCWGG 6 cut(s) 62, 245, 938, 1509, 1983, 2476
BseDI CCNNGG 8 cut(s) 90, 169, 936, 1281, 1982, 2290, 2457, 2474
BseJI GATNNNNATC 1 cut(s) 804
BseMI GCAATG 3 cut(s) 549, 2103, 2319
BseMII CTCAG 7 cut(s) 301, 1179, 1340, 1998, 2400, 2475, 2924
BseNI ACTGG 5 cut(s) 992, 1069, 1255, 1920, 2280
BsePI GCGCGC 1 cut(s) 1963
BseRI GAGGAG 5 cut(s) 418, 632, 1658, 1661, 2216
BseSI GKGCMC 1 cut(s) 1741
BseYI CCCAGC 3 cut(s) 51, 1958, 1967
BsgI GTGCAG 2 cut(s) 1770, 2601
Bsh1236I CGCG 3 cut(s) 810, 1150, 1965
BshFI GGCC 4 cut(s) 194, 1175, 2356, 2393
BshNI GGYRCC 3 cut(s) 447, 1736, 2928
BshTI ACCGGT 1 cut(s) 670
BsiHKCI CYCGRG 1 cut(s) 2704
BsiSI CCGG 8 cut(s) 278, 525, 671, 830, 891, 1256, 1523, 1865
BsmAI GTCTC 9 cut(s) 142, 203, 596, 1055, 1329, 1375, 2037, 2409, 2719
BsnI GGCC 4 cut(s) 194, 1175, 2356, 2393
Bso31I GGTCTC 3 cut(s) 142, 596, 2409
BsoBI CYCGRG 1 cut(s) 2704
Bsp1286I GDGCHC 3 cut(s) 937, 1741, 2919
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 8 cut(s) 269, 372, 910, 1148, 1972, 1996, 2336, 2843
BspANI GGCC 4 cut(s) 194, 1175, 2356, 2393
BspCNI CTCAG 7 cut(s) 300, 1180, 1339, 1999, 2401, 2476, 2925
BspFNI CGCG 3 cut(s) 810, 1150, 1965
BspHI TCATGA 2 cut(s) 1357, 2911
BspLI GGNNCC 8 cut(s) 449, 934, 1738, 2136, 2253, 2652, 2826, 2930
BspMI ACCTGC 2 cut(s) 1100, 2812
BspPI GGATC 9 cut(s) 449, 948, 1856, 2129, 2142, 2246, 2259, 2645, 2658
BspQI GCTCTTC 2 cut(s) 588, 726
BspT107I GGYRCC 3 cut(s) 447, 1736, 2928
BspTNI GGTCTC 3 cut(s) 142, 596, 2409
BsrBI CCGCTC 1 cut(s) 910
BsrDI GCAATG 3 cut(s) 549, 2103, 2319
BsrFI RCCGGY 1 cut(s) 670
BsrI ACTGG 5 cut(s) 992, 1069, 1255, 1920, 2280
BssAI RCCGGY 1 cut(s) 670
BssECI CCNNGG 8 cut(s) 90, 169, 936, 1281, 1982, 2290, 2457, 2474
BssHII GCGCGC 1 cut(s) 1963
BssNI GRCGYC 1 cut(s) 2929
BssSI CACGAG 1 cut(s) 905
BssT1I CCWWGG 3 cut(s) 90, 2290, 2457
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 6 cut(s) 62, 245, 938, 1509, 1983, 2476
Bst4CI ACNGT 9 cut(s) 1264, 1369, 1585, 2044, 2142, 2437, 2499, 2548, 2898
Bst6I CTCTTC 7 cut(s) 474, 570, 588, 726, 762, 1147, 1784
BstACI GRCGYC 1 cut(s) 2929
BstAPI GCANNNNNTGC 1 cut(s) 2618
BstBAI YACGTR 1 cut(s) 1855
BstC8I GCNNGC 6 cut(s) 883, 921, 1776, 1965, 2277, 2946
BstDEI CTNAG 9 cut(s) 287, 1188, 1326, 2007, 2057, 2409, 2484, 2856, 2933
BstDSI CCRYGG 1 cut(s) 90
BstFNI CGCG 3 cut(s) 810, 1150, 1965
BstH2I RGCGCY 6 cut(s) 59, 734, 982, 2016, 2156, 2932
BstHHI GCGC 9 cut(s) 58, 733, 812, 981, 1965, 1967, 2015, 2155, 2931
BstMAI GTCTC 9 cut(s) 142, 203, 596, 1055, 1329, 1375, 2037, 2409, 2719
BstNI CCWGG 6 cut(s) 62, 245, 938, 1509, 1983, 2476
BstNSI RCATGY 2 cut(s) 277, 2782
BstSCI CCNGG 8 cut(s) 60, 243, 829, 936, 1507, 1864, 1981, 2474
BstSFI CTRYAG 1 cut(s) 2350
BstSLI GKGCMC 1 cut(s) 1741
BstSNI TACGTA 1 cut(s) 1855
BstUI CGCG 3 cut(s) 810, 1150, 1965
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 4 cut(s) 940, 2134, 2251, 2650
BstXI CCANNNNNNTGG 1 cut(s) 1171
BstYI RGATCY 4 cut(s) 940, 2134, 2251, 2650
Bsu36I CCTNAGG 1 cut(s) 2409
BsuI GTATCC 4 cut(s) 644, 1252, 1281, 2097
BsuRI GGCC 4 cut(s) 194, 1175, 2356, 2393
BtgI CCRYGG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 1167
BtsI GCAGTG 2 cut(s) 1467, 2305
BtsIMutI CAGTG 8 cut(s) 1248, 1374, 1467, 2004, 2287, 2305, 2433, 2797
BveI ACCTGC 2 cut(s) 1100, 2812
Cac8I GCNNGC 6 cut(s) 883, 921, 1776, 1965, 2277, 2946
CciI TCATGA 2 cut(s) 1357, 2911
CfoI GCGC 9 cut(s) 58, 733, 812, 981, 1965, 1967, 2015, 2155, 2931
Cfr10I RCCGGY 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 1253, 1979
Csp6I GTAC 9 cut(s) 448, 1225, 1232, 1273, 1330, 1537, 1691, 2776, 2886
CspAI ACCGGT 1 cut(s) 670
CviQI GTAC 9 cut(s) 448, 1225, 1232, 1273, 1330, 1537, 1691, 2776, 2886
DdeI CTNAG 9 cut(s) 287, 1188, 1326, 2007, 2057, 2409, 2484, 2856, 2933
DinI GGCGCC 1 cut(s) 2930
EaeI YGGCCR 2 cut(s) 192, 1173
Eam1104I CTCTTC 7 cut(s) 474, 570, 588, 726, 762, 1147, 1784
EarI CTCTTC 7 cut(s) 474, 570, 588, 726, 762, 1147, 1784
EciI GGCGGA 1 cut(s) 1987
Eco105I TACGTA 1 cut(s) 1855
Eco130I CCWWGG 3 cut(s) 90, 2290, 2457
Eco147I AGGCCT 1 cut(s) 2356
Eco24I GRGCYC 2 cut(s) 937, 2919
Eco31I GGTCTC 3 cut(s) 142, 596, 2409
Eco32I GATATC 2 cut(s) 2527, 2909
Eco47I GGWCC 2 cut(s) 1253, 1979
Eco47III AGCGCT 3 cut(s) 732, 2014, 2154
Eco57I CTGAAG 6 cut(s) 1125, 1418, 1767, 1842, 2315, 2617
Eco81I CCTNAGG 1 cut(s) 2409
Eco88I CYCGRG 1 cut(s) 2704
EcoRII CCWGG 6 cut(s) 60, 243, 936, 1507, 1981, 2474
EcoRV GATATC 2 cut(s) 2527, 2909
EcoT14I CCWWGG 3 cut(s) 90, 2290, 2457
EcoT38I GRGCYC 2 cut(s) 937, 2919
EgeI GGCGCC 1 cut(s) 2930
EheI GGCGCC 1 cut(s) 2930
ErhI CCWWGG 3 cut(s) 90, 2290, 2457
FalI AAGNNNNNCTT 1 cut(s) 2932
FauI CCCGC 1 cut(s) 2329
FbaI TGATCA 2 cut(s) 1266, 1338
FblI GTMKAC 1 cut(s) 234
FriOI GRGCYC 2 cut(s) 937, 2919
FspBI CTAG 3 cut(s) 2186, 2358, 2675
GlaI GCGC 9 cut(s) 57, 732, 811, 980, 1964, 1966, 2014, 2154, 2930
GsaI CCCAGC 3 cut(s) 55, 1962, 1971
GsuI CTGGAG 2 cut(s) 44, 1692
HaeII RGCGCY 6 cut(s) 59, 734, 982, 2016, 2156, 2932
HaeIII GGCC 4 cut(s) 194, 1175, 2356, 2393
HapII CCGG 8 cut(s) 278, 525, 671, 830, 891, 1256, 1523, 1865
HhaI GCGC 9 cut(s) 58, 733, 812, 981, 1965, 1967, 2015, 2155, 2931
Hin1I GRCGYC 1 cut(s) 2929
Hin6I GCGC 9 cut(s) 56, 731, 810, 979, 1963, 1965, 2013, 2153, 2929
HinP1I GCGC 9 cut(s) 56, 731, 810, 979, 1963, 1965, 2013, 2153, 2929
HincII GTYRAC 2 cut(s) 67, 235
HindII GTYRAC 2 cut(s) 67, 235
HindIII AAGCTT 1 cut(s) 1898
HpaII CCGG 8 cut(s) 278, 525, 671, 830, 891, 1256, 1523, 1865
HphI GGTGA 5 cut(s) 660, 851, 1070, 1628, 1652
Hpy166II GTNNAC 4 cut(s) 67, 235, 997, 2440
Hpy8I GTNNAC 4 cut(s) 67, 235, 997, 2440
Hpy99I CGWCG 2 cut(s) 230, 239
HpyCH4III ACNGT 9 cut(s) 1264, 1369, 1585, 2044, 2142, 2437, 2499, 2548, 2898
HpyCH4IV ACGT 5 cut(s) 228, 1166, 1380, 1854, 2884
HpyF3I CTNAG 9 cut(s) 287, 1188, 1326, 2007, 2057, 2409, 2484, 2856, 2933
HpySE526I ACGT 5 cut(s) 228, 1166, 1380, 1854, 2884
Hsp92I GRCGYC 1 cut(s) 2929
HspAI GCGC 9 cut(s) 56, 731, 810, 979, 1963, 1965, 2013, 2153, 2929
KasI GGCGCC 1 cut(s) 2928
KpnI GGTACC 1 cut(s) 451
Ksp22I TGATCA 2 cut(s) 1266, 1338
LguI GCTCTTC 2 cut(s) 588, 726
LweI GCATC 5 cut(s) 1447, 1558, 1949, 2285, 2615
MaeI CTAG 3 cut(s) 2186, 2358, 2675
MaeII ACGT 5 cut(s) 228, 1166, 1380, 1854, 2884
MaeIII GTNAC 8 cut(s) 666, 1112, 1438, 1616, 1921, 1991, 2208, 2311
MbiI CCGCTC 1 cut(s) 910
MflI RGATCY 4 cut(s) 940, 2134, 2251, 2650
MhlI GDGCHC 3 cut(s) 937, 1741, 2919
MlsI TGGCCA 1 cut(s) 1175
MluNI TGGCCA 1 cut(s) 1175
Mly113I GGCGCC 1 cut(s) 2929
MlyI GAGTC 3 cut(s) 195, 715, 2168
MmeI TCCRAC 3 cut(s) 165, 204, 249
Mox20I TGGCCA 1 cut(s) 1175
MroXI GAANNNNTTC 3 cut(s) 396, 1017, 1766
MscI TGGCCA 1 cut(s) 1175
MseI TTAA 5 cut(s) 254, 1295, 1532, 1890, 2838
MslI CAYNNNNRTG 3 cut(s) 1169, 1625, 2324
Msp20I TGGCCA 1 cut(s) 1175
MspA1I CMGCKG 2 cut(s) 514, 2176
MspI CCGG 8 cut(s) 278, 525, 671, 830, 891, 1256, 1523, 1865
MspR9I CCNGG 8 cut(s) 62, 245, 831, 938, 1509, 1866, 1983, 2476
MvaI CCWGG 6 cut(s) 62, 245, 938, 1509, 1983, 2476
MvnI CGCG 3 cut(s) 810, 1150, 1965
NarI GGCGCC 1 cut(s) 2929
NciI CCSGG 2 cut(s) 831, 1866
NcoI CCATGG 1 cut(s) 90
NlaIV GGNNCC 8 cut(s) 449, 934, 1738, 2136, 2253, 2652, 2826, 2930
NmeAIII GCCGAG 3 cut(s) 150, 220, 232
NmuCI GTSAC 3 cut(s) 666, 1616, 2311
NspI RCATGY 2 cut(s) 277, 2782
OliI CACNNNNGTG 1 cut(s) 1169
PagI TCATGA 2 cut(s) 1357, 2911
PauI GCGCGC 1 cut(s) 1963
PceI AGGCCT 1 cut(s) 2356
PciSI GCTCTTC 2 cut(s) 588, 726
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 234
PdmI GAANNNNTTC 3 cut(s) 396, 1017, 1766
PfeI GAWTC 8 cut(s) 397, 473, 688, 805, 1018, 1504, 1651, 2832
PflFI GACNNNGTC 2 cut(s) 185, 230
PflMI CCANNNNNTGG 3 cut(s) 1733, 1901, 2925
PfoI TCCNGGA 1 cut(s) 1864
PinAI ACCGGT 1 cut(s) 670
PleI GAGTC 3 cut(s) 194, 714, 2167
PluTI GGCGCC 1 cut(s) 2932
PpsI GAGTC 3 cut(s) 194, 714, 2167
Ppu21I YACGTR 1 cut(s) 1855
PshBI ATTAAT 1 cut(s) 1532
Psp6I CCWGG 6 cut(s) 60, 243, 936, 1507, 1981, 2474
PspFI CCCAGC 3 cut(s) 51, 1958, 1967
PspGI CCWGG 6 cut(s) 60, 243, 936, 1507, 1981, 2474
PspN4I GGNNCC 8 cut(s) 449, 934, 1738, 2136, 2253, 2652, 2826, 2930
PspPI GGNCC 2 cut(s) 1253, 1979
PsuI RGATCY 4 cut(s) 940, 2134, 2251, 2650
PsyI GACNNNGTC 2 cut(s) 185, 230
PteI GCGCGC 1 cut(s) 1963
PvuII CAGCTG 2 cut(s) 514, 2176
RsaI GTAC 9 cut(s) 449, 1226, 1233, 1274, 1331, 1538, 1692, 2777, 2887
RsaNI GTAC 9 cut(s) 448, 1225, 1232, 1273, 1330, 1537, 1691, 2776, 2886
RseI CAYNNNNRTG 3 cut(s) 1169, 1625, 2324
SalI GTCGAC 1 cut(s) 233
SapI GCTCTTC 2 cut(s) 588, 726
SaqAI TTAA 5 cut(s) 254, 1295, 1532, 1890, 2838
Sau96I GGNCC 2 cut(s) 1253, 1979
ScaI AGTACT 2 cut(s) 1331, 1692
SchI GAGTC 3 cut(s) 195, 715, 2168
ScrFI CCNGG 8 cut(s) 62, 245, 831, 938, 1509, 1866, 1983, 2476
SduI GDGCHC 3 cut(s) 937, 1741, 2919
SfaNI GCATC 5 cut(s) 1447, 1558, 1949, 2285, 2615
SfcI CTRYAG 1 cut(s) 2350
SfoI GGCGCC 1 cut(s) 2930
SinI GGWCC 2 cut(s) 1253, 1979
SmiMI CAYNNNNRTG 3 cut(s) 1169, 1625, 2324
SmlI CTYRAG 2 cut(s) 1935, 2156
SmoI CTYRAG 2 cut(s) 1935, 2156
SnaBI TACGTA 1 cut(s) 1855
SseBI AGGCCT 1 cut(s) 2356
SsiI CCGC 8 cut(s) 269, 372, 910, 1148, 1972, 1996, 2336, 2843
SspDI GGCGCC 1 cut(s) 2928
SspI AATATT 1 cut(s) 1816
SspMI CTAG 3 cut(s) 2186, 2358, 2675
StuI AGGCCT 1 cut(s) 2356
StyD4I CCNGG 8 cut(s) 60, 243, 829, 936, 1507, 1864, 1981, 2474
StyI CCWWGG 3 cut(s) 90, 2290, 2457
TaaI ACNGT 9 cut(s) 1264, 1369, 1585, 2044, 2142, 2437, 2499, 2548, 2898
TaiI ACGT 5 cut(s) 231, 1169, 1383, 1857, 2887
TaqII GACCGA 3 cut(s) 620, 1295, 2493
TatI WGTACW 5 cut(s) 1272, 1329, 1536, 1690, 2775
TauI GCSGC 2 cut(s) 374, 2846
TfiI GAWTC 8 cut(s) 397, 473, 688, 805, 1018, 1504, 1651, 2832
Tru1I TTAA 5 cut(s) 254, 1295, 1532, 1890, 2838
Tru9I TTAA 5 cut(s) 254, 1295, 1532, 1890, 2838
TscAI CASTG 8 cut(s) 1255, 1381, 1467, 2011, 2287, 2305, 2440, 2797
TseFI GTSAC 3 cut(s) 666, 1616, 2311
Tsp45I GTSAC 3 cut(s) 666, 1616, 2311
TspGWI ACGGA 1 cut(s) 1324
TspRI CASTG 8 cut(s) 1255, 1381, 1467, 2011, 2287, 2305, 2440, 2797
Tth111I GACNNNGTC 2 cut(s) 185, 230
Van91I CCANNNNNTGG 3 cut(s) 1733, 1901, 2925
VpaK11BI GGWCC 2 cut(s) 1253, 1979
VspI ATTAAT 1 cut(s) 1532
XapI RAATTY 2 cut(s) 1193, 1302
XceI RCATGY 2 cut(s) 277, 2782
XcmI CCANNNNNNNNNTGG 1 cut(s) 2812
XmiI GTMKAC 1 cut(s) 234
XmnI GAANNNNTTC 3 cut(s) 396, 1017, 1766
XspI CTAG 3 cut(s) 2186, 2358, 2675
ZrmI AGTACT 2 cut(s) 1331, 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.