Rroxscaffold_4G00303550

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000671
Physical Location & Seq
Reverse (-)
23930981 .. 23938023
7043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303550.1

Sequence Viewer

Length: 3606 bp
ATGGCTGCGATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCCGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTCGACGCCAAATTGAAGGAACATGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCATGCAATTCTTCCTCCTAAGGCAAAGTCCGAGAATGACTCAAAGAAAGAGAGCACTGCAGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGTTGGAGGCCAAGGAAAAGCGAAATGACAGAGAAGAATGGGGAGATGGGCGTGAAGAAGATAGGCCCAGAGGTAGAGATGGAGGTAGAGGTAGGGGTAGGGAAAGAGATACAAATCGAGACAGAGACAGGGACAGGGACAGGGACAGGGACAGGGATAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAATCATTATCATGATTATGATAAGGGAAGGCATACAGATCGATCCAATAAGCACAGGAGGGATGAGTATGAAAAGGATGGAGATGTTAGAGAAGATGATGATGATAGGAGAGGAAGTAGGGATCAGCGGAATGGATTGCACCATTCTGATGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCACAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCACGTTTCGCAGATTGCTACTCGGCGAATTGGCAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTTAAGGTGATTTCAATTTCTGGTCAAAAGATGAGCCTGTCAATGAGGGATGTTGATCAGCATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCATCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCCGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTTTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACGCAGTATCTTGCAGAAGCTGGGTACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCGATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTCTGCTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATATGGACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATCCCTGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCGGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCGCTATATGAGAGGATGAAAGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCACTGACCATTGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAACAACGAGCTGGGCGTGCTGGCCGTACAGGGCCCGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTACTACATTAATAATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCGGGGCCTTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCCTTAGGAGGGCGCAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGACGTTGTGAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGCTGCTAGGAAGGACCCACAGGAGGGGTATAGAACCCTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCTCTCTTCCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGACAGTTGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCACGGTGGCTGATCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAACGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCCTGGCGTCTTAGTAAACGGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1201

Amino Acids

137.41

Weight (kDa)

6.39

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 249 - 316 8e-12 S1 RNA binding domain
DEAD PF00270 555 - 704 3.7e-08 DEAD/DEAH box helicase
AAA_22 PF13401 563 - 696 2.1e-06 AAA domain
Helicase_C PF00271 748 - 878 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 940 - 968 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 969 - 1028 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1086 - 1162 1e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 753, 2544
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1499, 3409
AciI CCGC 8 cut(s) 571, 706, 1320, 2068, 2650, 2674, 3436, 3548
AcoI YGGCCR 5 cut(s) 1118, 2598, 2623, 2889, 2931
AcsI RAATTY 4 cut(s) 152, 2247, 2706, 3244
AcuI CTGAAG 3 cut(s) 1679, 1863, 2679
AcyI GRCGYC 2 cut(s) 159, 3583
AfaI GTAC 7 cut(s) 38, 208, 1769, 1795, 1928, 1985, 2629
AhlI ACTAGT 1 cut(s) 2569
AjnI CCWGG 8 cut(s) 1310, 1737, 2161, 2437, 2518, 3027, 3482, 3578
AjuI GAANNNNNNNTTGG 4 cut(s) 809, 841, 1652, 1684
AleI CACNNNNGTG 1 cut(s) 770
Alw21I GWGCWC 3 cut(s) 281, 1139, 3370
Alw26I GTCTC 8 cut(s) 477, 483, 1073, 1490, 1709, 2065, 3159, 3373
AlwNI CAGNNNCTG 2 cut(s) 1499, 1763
Ama87I CYCGRG 3 cut(s) 32, 1174, 2636
ApaI GGGCCC 1 cut(s) 2637
ApeKI GCWGC 8 cut(s) 5, 1376, 1379, 1426, 1429, 1814, 2200, 3293
ApoI RAATTY 4 cut(s) 152, 2247, 2706, 3244
ArsI GACNNNNNNTTYG 4 cut(s) 2064, 2096, 2507, 2539
AseI ATTAAT 1 cut(s) 2738
Asp700I GAANNNNTTC 1 cut(s) 1671
AspA2I CCTAGG 1 cut(s) 3011
AspLEI GCGC 2 cut(s) 3175, 3525
AspS9I GGNCC 8 cut(s) 429, 1049, 1907, 2633, 2634, 2941, 3125, 3304
AsuC2I CCSGG 2 cut(s) 2637, 2638
AsuHPI GGTGA 9 cut(s) 892, 922, 1131, 1323, 1514, 1724, 2282, 2790, 3454
AvaI CYCGRG 3 cut(s) 32, 1174, 2636
AvaII GGWCC 3 cut(s) 1907, 2941, 3304
AvrII CCTAGG 1 cut(s) 3011
AxyI CCTNAGG 2 cut(s) 243, 3164
BaeGI GKGCMC 2 cut(s) 2395, 2637
BalI TGGCCA 1 cut(s) 2933
BamHI GGATCC 2 cut(s) 2788, 2905
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 3 cut(s) 1139, 2637, 2848
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1592, 2855
Bbv12I GWGCWC 3 cut(s) 281, 1139, 3370
BbvCI CCTCAGC 1 cut(s) 1251
BbvI GCAGC 7 cut(s) 1366, 1388, 1438, 1441, 1801, 2212, 3280
BceAI ACGGC 1 cut(s) 2610
BcgI CGANNNNNNTGC 4 cut(s) 599, 633, 2304, 2338
BciT130I CCWGG 8 cut(s) 1312, 1739, 2163, 2439, 2520, 3029, 3484, 3580
BciVI GTATCC 1 cut(s) 1209
BclI TGATCA 2 cut(s) 958, 3034
BcnI CCSGG 2 cut(s) 2637, 2638
BcoDI GTCTC 8 cut(s) 477, 483, 1073, 1490, 1709, 2065, 3159, 3373
BcuI ACTAGT 1 cut(s) 2569
BfaI CTAG 9 cut(s) 83, 104, 2561, 2570, 2849, 3012, 3297, 3329, 3476
BfmI CTRYAG 4 cut(s) 282, 1824, 2178, 3273
BfuI GTATCC 1 cut(s) 1209
BisI GCNGC 8 cut(s) 6, 1377, 1380, 1427, 1430, 1815, 2201, 3294
BlnI CCTAGG 1 cut(s) 3011
BlsI GCNGC 8 cut(s) 7, 1378, 1381, 1428, 1431, 1816, 2202, 3295
BmcAI AGTACT 1 cut(s) 1985
Bme18I GGWCC 3 cut(s) 1907, 2941, 3304
BmeT110I CYCGRG 3 cut(s) 32, 1174, 2636
BmgT120I GGNCC 8 cut(s) 429, 1049, 1907, 2633, 2634, 2941, 3125, 3304
BmiI GGNNCC 8 cut(s) 181, 2262, 2635, 2790, 2845, 2907, 3126, 3306
BmrI ACTGGG 2 cut(s) 978, 2394
BmuI ACTGGG 2 cut(s) 978, 2394
BpiI GAAGAC 2 cut(s) 1592, 2855
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1251
BpuEI CTTGAG 1 cut(s) 1283
BpuMI CCSGG 2 cut(s) 2637, 2638
Bsa29I ATCGAT 2 cut(s) 619, 1031
BsaAI YACGTR 1 cut(s) 1805
BsaBI GATNNNNATC 3 cut(s) 477, 957, 1467
BsaHI GRCGYC 2 cut(s) 159, 3583
BsaI GGTCTC 2 cut(s) 1073, 2065
BsaWI WCCGGW 2 cut(s) 1141, 3385
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bse118I RCCGGY 1 cut(s) 1116
Bse21I CCTNAGG 2 cut(s) 243, 3164
Bse3DI GCAATG 4 cut(s) 871, 2683, 2757, 2973
Bse8I GATNNNNATC 3 cut(s) 477, 957, 1467
BseBI CCWGG 8 cut(s) 1312, 1739, 2163, 2439, 2520, 3029, 3484, 3580
BseCI ATCGAT 2 cut(s) 619, 1031
BseJI GATNNNNATC 3 cut(s) 477, 957, 1467
BseMI GCAATG 4 cut(s) 871, 2683, 2757, 2973
BseMII CTCAG 8 cut(s) 111, 1014, 1242, 1551, 1994, 2106, 2355, 2652
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 2 cut(s) 2395, 2637
BseXI GCAGC 7 cut(s) 1366, 1388, 1438, 1441, 1801, 2212, 3280
BseYI CCCAGC 2 cut(s) 1763, 2612
BsgI GTGCAG 3 cut(s) 1365, 1377, 1395
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 2 cut(s) 619, 1031
BsiHKAI GWGCWC 3 cut(s) 281, 1139, 3370
BsiHKCI CYCGRG 3 cut(s) 32, 1174, 2636
BsiSI CCGG 5 cut(s) 1117, 1142, 2264, 2637, 3386
BslFI GGGAC 9 cut(s) 107, 141, 509, 515, 521, 527, 2307, 2671, 2740
BsmAI GTCTC 8 cut(s) 477, 483, 1073, 1490, 1709, 2065, 3159, 3373
BsmFI GGGAC 9 cut(s) 107, 141, 509, 515, 521, 527, 2307, 2671, 2740
Bso31I GGTCTC 2 cut(s) 1073, 2065
BsoBI CYCGRG 3 cut(s) 32, 1174, 2636
Bsp120I GGGCCC 1 cut(s) 2633
Bsp1286I GDGCHC 6 cut(s) 281, 1139, 2395, 2637, 2848, 3370
Bsp1407I TGTACA 1 cut(s) 1793
BspACI CCGC 8 cut(s) 571, 706, 1320, 2068, 2650, 2674, 3436, 3548
BspCNI CTCAG 8 cut(s) 112, 1013, 1243, 1552, 1993, 2107, 2356, 2653
BspDI ATCGAT 2 cut(s) 619, 1031
BspHI TCATGA 2 cut(s) 589, 2011
BspLI GGNNCC 8 cut(s) 181, 2262, 2635, 2790, 2845, 2907, 3126, 3306
BspMAI CTGCAG 2 cut(s) 286, 3277
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 2 cut(s) 1073, 2065
BsrDI GCAATG 4 cut(s) 871, 2683, 2757, 2973
BsrFI RCCGGY 1 cut(s) 1116
BsrGI TGTACA 1 cut(s) 1793
BssAI RCCGGY 1 cut(s) 1116
BssNI GRCGYC 2 cut(s) 159, 3583
BssT1I CCWWGG 8 cut(s) 45, 54, 375, 1475, 2106, 2944, 3011, 3128
Bst2UI CCWGG 8 cut(s) 1312, 1739, 2163, 2439, 2520, 3029, 3484, 3580
Bst4CI ACNGT 9 cut(s) 137, 1164, 1591, 1918, 2144, 2239, 2837, 3448, 3496
Bst6I CTCTTC 9 cut(s) 878, 1086, 1121, 1236, 1839, 2438, 2850, 3035, 3377
BstACI GRCGYC 2 cut(s) 159, 3583
BstAPI GCANNNNNTGC 2 cut(s) 1642, 3272
BstAUI TGTACA 1 cut(s) 1793
BstBAI YACGTR 1 cut(s) 1805
BstHHI GCGC 2 cut(s) 3175, 3525
BstMAI GTCTC 8 cut(s) 477, 483, 1073, 1490, 1709, 2065, 3159, 3373
BstMWI GCNNNNNNNGC 5 cut(s) 1426, 1642, 2618, 3107, 3272
BstNI CCWGG 8 cut(s) 1312, 1739, 2163, 2439, 2520, 3029, 3484, 3580
BstNSI RCATGY 2 cut(s) 1558, 2319
BstSFI CTRYAG 4 cut(s) 282, 1824, 2178, 3273
BstSLI GKGCMC 2 cut(s) 2395, 2637
BstV1I GCAGC 7 cut(s) 1366, 1388, 1438, 1441, 1801, 2212, 3280
BstV2I GAAGAC 2 cut(s) 1592, 2855
BstX2I RGATCY 5 cut(s) 1462, 1483, 2788, 2905, 2961
BstXI CCANNNNNNTGG 1 cut(s) 3579
BstYI RGATCY 5 cut(s) 1462, 1483, 2788, 2905, 2961
Bsu15I ATCGAT 2 cut(s) 619, 1031
Bsu36I CCTNAGG 2 cut(s) 243, 3164
BsuI GTATCC 1 cut(s) 1209
BsuTUI ATCGAT 2 cut(s) 619, 1031
BtgZI GCGATG 1 cut(s) 1832
BtsI GCAGTG 4 cut(s) 279, 1686, 2959, 3370
BtsIMutI CAGTG 9 cut(s) 279, 1686, 1902, 2235, 2407, 2483, 2658, 2959, 3370
CaiI CAGNNNCTG 2 cut(s) 1499, 1763
CciI TCATGA 2 cut(s) 589, 2011
CfoI GCGC 2 cut(s) 3175, 3525
Cfr10I RCCGGY 1 cut(s) 1116
Cfr13I GGNCC 8 cut(s) 429, 1049, 1907, 2633, 2634, 2941, 3125, 3304
Cfr9I CCCGGG 1 cut(s) 2636
ClaI ATCGAT 2 cut(s) 619, 1031
CseI GACGC 3 cut(s) 167, 3518, 3572
CsiI ACCWGGT 1 cut(s) 1310
Csp6I GTAC 7 cut(s) 37, 207, 1768, 1794, 1927, 1984, 2628
CviQI GTAC 7 cut(s) 37, 207, 1768, 1794, 1927, 1984, 2628
EaeI YGGCCR 5 cut(s) 1118, 2598, 2623, 2889, 2931
Eam1104I CTCTTC 9 cut(s) 878, 1086, 1121, 1236, 1839, 2438, 2850, 3035, 3377
EarI CTCTTC 9 cut(s) 878, 1086, 1121, 1236, 1839, 2438, 2850, 3035, 3377
EciI GGCGGA 1 cut(s) 586
Ecl136II GAGCTC 1 cut(s) 1137
Eco130I CCWWGG 8 cut(s) 45, 54, 375, 1475, 2106, 2944, 3011, 3128
Eco147I AGGCCT 1 cut(s) 3010
Eco24I GRGCYC 3 cut(s) 1139, 2637, 2848
Eco31I GGTCTC 2 cut(s) 1073, 2065
Eco32I GATATC 2 cut(s) 349, 1035
Eco47I GGWCC 3 cut(s) 1907, 2941, 3304
Eco53kI GAGCTC 1 cut(s) 1137
Eco57I CTGAAG 3 cut(s) 1679, 1863, 2679
Eco81I CCTNAGG 2 cut(s) 243, 3164
Eco88I CYCGRG 3 cut(s) 32, 1174, 2636
EcoICRI GAGCTC 1 cut(s) 1137
EcoO109I RGGNCCY 3 cut(s) 2633, 3125, 3304
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 8 cut(s) 1310, 1737, 2161, 2437, 2518, 3027, 3482, 3578
EcoRV GATATC 2 cut(s) 349, 1035
EcoT14I CCWWGG 8 cut(s) 45, 54, 375, 1475, 2106, 2944, 3011, 3128
EcoT22I ATGCAT 1 cut(s) 2227
EcoT38I GRGCYC 3 cut(s) 1139, 2637, 2848
ErhI CCWWGG 8 cut(s) 45, 54, 375, 1475, 2106, 2944, 3011, 3128
FalI AAGNNNNNCTT 2 cut(s) 2847, 2879
FaqI GGGAC 9 cut(s) 107, 141, 509, 515, 521, 527, 2307, 2671, 2740
FauI CCCGC 1 cut(s) 3429
FbaI TGATCA 2 cut(s) 958, 3034
Fnu4HI GCNGC 8 cut(s) 6, 1377, 1380, 1427, 1430, 1815, 2201, 3294
FriOI GRGCYC 3 cut(s) 1139, 2637, 2848
Fsp4HI GCNGC 8 cut(s) 6, 1377, 1380, 1427, 1430, 1815, 2201, 3294
FspBI CTAG 9 cut(s) 83, 104, 2561, 2570, 2849, 3012, 3297, 3329, 3476
GlaI GCGC 2 cut(s) 3174, 3524
GluI GCNGC 8 cut(s) 6, 1377, 1380, 1427, 1430, 1815, 2201, 3294
GsaI CCCAGC 2 cut(s) 1767, 2616
HapII CCGG 5 cut(s) 1117, 1142, 2264, 2637, 3386
HgaI GACGC 3 cut(s) 167, 3518, 3572
HhaI GCGC 2 cut(s) 3175, 3525
Hin1I GRCGYC 2 cut(s) 159, 3583
Hin6I GCGC 2 cut(s) 3173, 3523
HinP1I GCGC 2 cut(s) 3173, 3523
HincII GTYRAC 1 cut(s) 3454
HindII GTYRAC 1 cut(s) 3454
HindIII AAGCTT 1 cut(s) 1613
HinfI GANTC 9 cut(s) 263, 1010, 1351, 1369, 1448, 1468, 1957, 2564, 2983
HpaII CCGG 5 cut(s) 1117, 1142, 2264, 2637, 3386
HphI GGTGA 9 cut(s) 892, 922, 1131, 1323, 1514, 1724, 2282, 2790, 3454
Hpy166II GTNNAC 8 cut(s) 209, 832, 1770, 1794, 1796, 2941, 3454, 3593
Hpy8I GTNNAC 8 cut(s) 209, 832, 1770, 1794, 1796, 2941, 3454, 3593
Hpy99I CGWCG 1 cut(s) 161
HpyCH4III ACNGT 9 cut(s) 137, 1164, 1591, 1918, 2144, 2239, 2837, 3448, 3496
HpyCH4IV ACGT 4 cut(s) 834, 1804, 3225, 3538
HpyF10VI GCNNNNNNNGC 5 cut(s) 1426, 1642, 2618, 3107, 3272
HpySE526I ACGT 4 cut(s) 834, 1804, 3225, 3538
Hsp92I GRCGYC 2 cut(s) 159, 3583
HspAI GCGC 2 cut(s) 3173, 3523
KroI GCCGGC 1 cut(s) 1116
KroNI GCCGGC 1 cut(s) 1118
Ksp22I TGATCA 2 cut(s) 958, 3034
LmnI GCTCC 4 cut(s) 1247, 1405, 2260, 2843
Lsp1109I GCAGC 7 cut(s) 1366, 1388, 1438, 1441, 1801, 2212, 3280
MabI ACCWGGT 1 cut(s) 1310
MaeI CTAG 9 cut(s) 83, 104, 2561, 2570, 2849, 3012, 3297, 3329, 3476
MaeII ACGT 4 cut(s) 834, 1804, 3225, 3538
MflI RGATCY 5 cut(s) 1462, 1483, 2788, 2905, 2961
MhlI GDGCHC 6 cut(s) 281, 1139, 2395, 2637, 2848, 3370
MlsI TGGCCA 1 cut(s) 2933
MluNI TGGCCA 1 cut(s) 2933
MlyI GAGTC 2 cut(s) 257, 1378
MmeI TCCRAC 3 cut(s) 348, 1425, 1855
Mox20I TGGCCA 1 cut(s) 2933
Mph1103I ATGCAT 1 cut(s) 2227
MroNI GCCGGC 1 cut(s) 1116
MroXI GAANNNNTTC 1 cut(s) 1671
MscI TGGCCA 1 cut(s) 2933
MseI TTAA 7 cut(s) 906, 1275, 1347, 1401, 2231, 2255, 2738
MslI CAYNNNNRTG 6 cut(s) 588, 594, 726, 770, 1775, 2978
Msp20I TGGCCA 1 cut(s) 2933
MspA1I CMGCKG 2 cut(s) 706, 1379
MspI CCGG 5 cut(s) 1117, 1142, 2264, 2637, 3386
MvaI CCWGG 8 cut(s) 1312, 1739, 2163, 2439, 2520, 3029, 3484, 3580
MwoI GCNNNNNNNGC 5 cut(s) 1426, 1642, 2618, 3107, 3272
NaeI GCCGGC 1 cut(s) 1118
NciI CCSGG 2 cut(s) 2637, 2638
NgoMIV GCCGGC 1 cut(s) 1116
NlaIV GGNNCC 8 cut(s) 181, 2262, 2635, 2790, 2845, 2907, 3126, 3306
NmeAIII GCCGAG 5 cut(s) 133, 208, 832, 2500, 2917
NmuCI GTSAC 7 cut(s) 763, 1054, 1329, 1591, 2092, 2270, 3442
NsiI ATGCAT 1 cut(s) 2227
NspI RCATGY 2 cut(s) 1558, 2319
OliI CACNNNNGTG 1 cut(s) 770
PaeI GCATGC 1 cut(s) 2319
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 589, 2011
PceI AGGCCT 1 cut(s) 3010
PcsI WCGNNNNNNNCGW 1 cut(s) 2614
PdiI GCCGGC 1 cut(s) 1118
PdmI GAANNNNTTC 1 cut(s) 1671
PfeI GAWTC 7 cut(s) 1010, 1351, 1448, 1468, 1957, 2564, 2983
PflMI CCANNNNNTGG 2 cut(s) 1499, 3409
PkrI GCNGC 8 cut(s) 7, 1378, 1381, 1428, 1431, 1816, 2202, 3295
PleI GAGTC 2 cut(s) 257, 1377
PpsI GAGTC 2 cut(s) 257, 1377
Ppu21I YACGTR 1 cut(s) 1805
PpuMI RGGWCCY 1 cut(s) 3304
PshBI ATTAAT 1 cut(s) 2738
PsiI TTATAA 2 cut(s) 753, 2544
Psp124BI GAGCTC 1 cut(s) 1139
Psp5II RGGWCCY 1 cut(s) 3304
Psp6I CCWGG 8 cut(s) 1310, 1737, 2161, 2437, 2518, 3027, 3482, 3578
PspFI CCCAGC 2 cut(s) 1763, 2612
PspGI CCWGG 8 cut(s) 1310, 1737, 2161, 2437, 2518, 3027, 3482, 3578
PspN4I GGNNCC 8 cut(s) 181, 2262, 2635, 2790, 2845, 2907, 3126, 3306
PspOMI GGGCCC 1 cut(s) 2633
PspPI GGNCC 8 cut(s) 429, 1049, 1907, 2633, 2634, 2941, 3125, 3304
PspPPI RGGWCCY 1 cut(s) 3304
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 2 cut(s) 286, 3277
PstNI CAGNNNCTG 2 cut(s) 1499, 1763
PsuI RGATCY 5 cut(s) 1462, 1483, 2788, 2905, 2961
PvuII CAGCTG 1 cut(s) 1379
RsaI GTAC 7 cut(s) 38, 208, 1769, 1795, 1928, 1985, 2629
RsaNI GTAC 7 cut(s) 37, 207, 1768, 1794, 1927, 1984, 2628
RseI CAYNNNNRTG 6 cut(s) 588, 594, 726, 770, 1775, 2978
SacI GAGCTC 1 cut(s) 1139
SaqAI TTAA 7 cut(s) 906, 1275, 1347, 1401, 2231, 2255, 2738
SatI GCNGC 8 cut(s) 6, 1377, 1380, 1427, 1430, 1815, 2201, 3294
Sau96I GGNCC 8 cut(s) 429, 1049, 1907, 2633, 2634, 2941, 3125, 3304
ScaI AGTACT 1 cut(s) 1985
SchI GAGTC 2 cut(s) 257, 1378
SduI GDGCHC 6 cut(s) 281, 1139, 2395, 2637, 2848, 3370
SexAI ACCWGGT 1 cut(s) 1310
SfcI CTRYAG 4 cut(s) 282, 1824, 2178, 3273
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 3 cut(s) 1907, 2941, 3304
SlaI CTCGAG 1 cut(s) 32
SmaI CCCGGG 1 cut(s) 2638
SmiMI CAYNNNNRTG 6 cut(s) 588, 594, 726, 770, 1775, 2978
SmlI CTYRAG 2 cut(s) 32, 1262
SmoI CTYRAG 2 cut(s) 32, 1262
SpeI ACTAGT 1 cut(s) 2569
SphI GCATGC 1 cut(s) 2319
SseBI AGGCCT 1 cut(s) 3010
SsiI CCGC 8 cut(s) 571, 706, 1320, 2068, 2650, 2674, 3436, 3548
SspI AATATT 1 cut(s) 1344
SspMI CTAG 9 cut(s) 83, 104, 2561, 2570, 2849, 3012, 3297, 3329, 3476
SstI GAGCTC 1 cut(s) 1139
StuI AGGCCT 1 cut(s) 3010
StyI CCWWGG 8 cut(s) 45, 54, 375, 1475, 2106, 2944, 3011, 3128
TaaI ACNGT 9 cut(s) 137, 1164, 1591, 1918, 2144, 2239, 2837, 3448, 3496
TaiI ACGT 4 cut(s) 837, 1807, 3228, 3541
TaqI TCGA 8 cut(s) 33, 156, 481, 619, 1031, 2139, 2414, 3157
TatI WGTACW 3 cut(s) 1793, 1926, 1983
TfiI GAWTC 7 cut(s) 1010, 1351, 1448, 1468, 1957, 2564, 2983
Tru1I TTAA 7 cut(s) 906, 1275, 1347, 1401, 2231, 2255, 2738
Tru9I TTAA 7 cut(s) 906, 1275, 1347, 1401, 2231, 2255, 2738
TscAI CASTG 9 cut(s) 286, 1686, 1909, 2242, 2407, 2490, 2665, 2959, 3370
TseFI GTSAC 7 cut(s) 763, 1054, 1329, 1591, 2092, 2270, 3442
TseI GCWGC 8 cut(s) 5, 1376, 1379, 1426, 1429, 1814, 2200, 3293
Tsp45I GTSAC 7 cut(s) 763, 1054, 1329, 1591, 2092, 2270, 3442
TspGWI ACGGA 2 cut(s) 194, 2273
TspMI CCCGGG 1 cut(s) 2636
TspRI CASTG 9 cut(s) 286, 1686, 1909, 2242, 2407, 2490, 2665, 2959, 3370
Van91I CCANNNNNTGG 2 cut(s) 1499, 3409
VpaK11BI GGWCC 3 cut(s) 1907, 2941, 3304
VspI ATTAAT 1 cut(s) 2738
XapI RAATTY 4 cut(s) 152, 2247, 2706, 3244
XceI RCATGY 2 cut(s) 1558, 2319
XcmI CCANNNNNNNNNTGG 1 cut(s) 3466
XhoI CTCGAG 1 cut(s) 32
XmaI CCCGGG 1 cut(s) 2636
XmaJI CCTAGG 1 cut(s) 3011
XmnI GAANNNNTTC 1 cut(s) 1671
XspI CTAG 9 cut(s) 83, 104, 2561, 2570, 2849, 3012, 3297, 3329, 3476
ZrmI AGTACT 1 cut(s) 1985
Zsp2I ATGCAT 1 cut(s) 2227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.