FvH4_7g12750

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000671
Physical Location & Seq
Forward (+)
11565705 .. 11571852
6148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12750.t1

Sequence Viewer

Length: 3612 bp
ATGGTTGCCATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATTTAGGGTTTGGGGACAAAGTTCTAGCCGAGTTCATAACTGAAATGGGAAGAAACTGTGAGAGTGTGGATGAATTTGATGCCAAATTGAAGGAACACGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTGCTCACTATCATTCACGCAATTCTTCCTCCAAAGGCAAAGTCCGAGAACGACTCAAAGAAAGAGAGCACTGCTGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGATGGAGACCAAGGAAAAGCAAAATGACAGAGAAGAATGGGGAGATGAGCGTGAAGAAGATAGGCCCAGAGGTAGAGGTAGGGAAAGAGATACAGATCGAGAAAGAGAAAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACCGAGATAGGGATAGGGATAGGGACAGAAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGGGAAGAGATAGACATTATCATGATTATGATAAGGGAAGGCATACAGATCGTTCCAACAAGCACAGGAGGGATGATTATGTAGAGGATGGTGATGTTAGAGAAGACGATGATGGTGATAGGAGAGGAAGTGGAGATCAGCAGAATGGACTGCACCATTCTGAGGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTATCAAAAGTGATGGACACGGGTTGCTTTGTTCAGTTCAGCGACTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCAACTCGGCGAATTGGAAATGCTAAAGATGTGGTGAAGAGGGATCAAGAGGTCTATGTTAAGGTGATTTCAATATCTGGTCAAAAGTTGAGCCTTTCAATGAGAGATGTTGATCAGCATACTGGCCAGGATTTGCTTCCTCTGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCATCGATATCAAAGGATGAGGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCAGAAAAGTGGGAAGCCAAACAGCTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCCATGTATGATGAAGAAACAGATGGGATGCTTTTTGAAGAAGAAGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTTTTGCATGGGCAAACCAGGTATTCAGTAGATATGTCACCGGTGAAAATCTTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTAGATTCAATCCCAAAGGATCTAAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGATTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCACTTTTGGACAGAGGTCAAAGCTCTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGACAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACTCAGGTGACACAGTATCTTGCAGAAGCTGGTTACACTACAATGGGTAAGATTGGGTGTACACAACCACGTAGGGTGGCTGCGATGTCTGTAGCCAAGAGGGTCGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGGCCTGATACTGTAATCAAGTACATGACAGATGGTATGCTTCTTAGAGAGATACTGATTGACGAGAATCTTTCTCAATACTCTGTGGTTATGCTTGATGAAGCTCATGAGAGGACAATCTATACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGTAGGCCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCTGAGAAGTTTTCATCTTATTTCTTCAACTGTAACATTTTCACTATCCCCGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTACCTTGATGCATCGTTAATCACTGTTCTACAAATTCACTTAACAGAGCCGGAAGGTGACATCCTTCTCTTCTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTTTATGAGAGGATGAAGGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACTAATATAGCCGAGGCATCACTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTGGTCATTACCCCAATTTCACAAGCATCAGCCAAGCAGCGAGCTGGACGTGCTGGCCGTACTGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCTTACCGGAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTACTACACTTACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTTTACAGTTTAGGAGCGCTAGATGAAGAGGGTCTTCTGACCAAGTTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCGTTATCTAAGATGCTTCTGGCCAGTGTGGACCTTGGATGTAGTGATGAGATCCTGACTATCATTTCTATGATTCAGACAGGAAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCTTGGTGTTTTGAGAACTTCGTTCAGTCTCGATCCCTTAGGAGGGCGCAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGATGTTGTGAGTGCTGGAAAGAATTTTACGAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGGTGCTAGAAAGGACCCACAAGAGGGTTATAGAACCCTAGTGGAGAATCAGCCAGTTTATATCCATCCAAGCAGTGCTCTCTTCCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGACAGTTGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAGGTGGCTGATCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAACGTATTGAACCACTATATGATAGATACCATGAACCAAACTCCTGGCGTCTTAGCAAACGGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1204

Amino Acids

137.77

Weight (kDa)

6.01

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 252 - 319 4.3e-11 S1 RNA binding domain
DEAD PF00270 557 - 706 3.7e-07 DEAD/DEAH box helicase
AAA_22 PF13401 565 - 698 1.8e-06 AAA domain
Helicase_C PF00271 750 - 880 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 942 - 970 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 971 - 1030 8.8e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1088 - 1164 7.3e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 762, 2550
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1505, 3415
AciI CCGC 2 cut(s) 2656, 3442
AcoI YGGCCR 5 cut(s) 979, 1124, 2629, 2895, 2937
AcsI RAATTY 4 cut(s) 152, 2253, 2712, 3250
AcuI CTGAAG 5 cut(s) 306, 1019, 1685, 1869, 2685
AcyI GRCGYC 1 cut(s) 3589
AfaI GTAC 5 cut(s) 38, 208, 1801, 1934, 2635
AfeI AGCGCT 1 cut(s) 2853
AgeI ACCGGT 1 cut(s) 1339
AjiI CACGTC 1 cut(s) 2624
AjnI CCWGG 8 cut(s) 981, 1316, 2443, 2524, 2641, 3033, 3488, 3584
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 1658, 1690
Alw21I GWGCWC 3 cut(s) 281, 1145, 3376
AlwNI CAGNNNCTG 2 cut(s) 1505, 1769
Ama87I CYCGRG 2 cut(s) 32, 1180
Aor51HI AGCGCT 1 cut(s) 2853
ApeKI GCWGC 7 cut(s) 1382, 1385, 1432, 1435, 1820, 2206, 2611
ApoI RAATTY 4 cut(s) 152, 2253, 2712, 3250
ArsI GACNNNNNNTTYG 4 cut(s) 236, 268, 2513, 2545
AsiGI ACCGGT 1 cut(s) 1339
Asp700I GAANNNNTTC 1 cut(s) 1677
AspA2I CCTAGG 1 cut(s) 3017
AspLEI GCGC 3 cut(s) 2854, 3181, 3531
AspS9I GGNCC 7 cut(s) 429, 1055, 1913, 2639, 2947, 3131, 3310
AsuC2I CCSGG 1 cut(s) 2169
AvaI CYCGRG 2 cut(s) 32, 1180
AvaII GGWCC 2 cut(s) 2947, 3310
AvrII CCTAGG 1 cut(s) 3017
AxyI CCTNAGG 1 cut(s) 3170
BaeGI GKGCMC 1 cut(s) 2401
BalI TGGCCA 2 cut(s) 981, 2939
BamHI GGATCC 2 cut(s) 2794, 2911
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 1145
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 3 cut(s) 684, 1598, 2861
Bbv12I GWGCWC 3 cut(s) 281, 1145, 3376
BbvCI CCTCAGC 1 cut(s) 1257
BbvI GCAGC 7 cut(s) 1372, 1394, 1444, 1447, 1807, 2218, 2623
BceAI ACGGC 1 cut(s) 2616
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 8 cut(s) 983, 1318, 2445, 2526, 2643, 3035, 3490, 3586
BciVI GTATCC 1 cut(s) 1215
BclI TGATCA 2 cut(s) 967, 3040
BcnI CCSGG 1 cut(s) 2169
BfaI CTAG 8 cut(s) 104, 2406, 2567, 2855, 3018, 3303, 3335, 3482
BfmI CTRYAG 3 cut(s) 1830, 2184, 3279
BfoI RGCGCY 1 cut(s) 2855
BfuI GTATCC 1 cut(s) 1215
BisI GCNGC 7 cut(s) 1383, 1386, 1433, 1436, 1821, 2207, 2612
BlnI CCTAGG 1 cut(s) 3017
BlsI GCNGC 7 cut(s) 1384, 1387, 1434, 1437, 1822, 2208, 2613
Bme1390I CCNGG 9 cut(s) 983, 1318, 2169, 2445, 2526, 2643, 3035, 3490, 3586
Bme18I GGWCC 2 cut(s) 2947, 3310
BmeT110I CYCGRG 2 cut(s) 32, 1180
BmgBI CACGTC 1 cut(s) 2624
BmgT120I GGNCC 7 cut(s) 429, 1055, 1913, 2639, 2947, 3131, 3310
BmiI GGNNCC 6 cut(s) 181, 741, 1056, 2796, 2913, 3312
BmrFI CCNGG 9 cut(s) 983, 1318, 2169, 2445, 2526, 2643, 3035, 3490, 3586
BmrI ACTGGG 2 cut(s) 1920, 2646
BmuI ACTGGG 2 cut(s) 1920, 2646
BoxI GACNNNNGTC 1 cut(s) 1612
BpiI GAAGAC 3 cut(s) 684, 1598, 2861
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 1 cut(s) 1289
BpuMI CCSGG 1 cut(s) 2169
Bsa29I ATCGAT 2 cut(s) 1037, 2499
BsaAI YACGTR 1 cut(s) 1811
BsaBI GATNNNNATC 3 cut(s) 459, 966, 1473
BsaHI GRCGYC 1 cut(s) 3589
BsaI GGTCTC 3 cut(s) 365, 501, 1079
BsaWI WCCGGW 3 cut(s) 1339, 2679, 3391
BsaXI ACNNNNNCTCC 2 cut(s) 688, 718
Bse118I RCCGGY 2 cut(s) 1122, 1339
Bse21I CCTNAGG 1 cut(s) 3170
Bse3DI GCAATG 1 cut(s) 2763
Bse8I GATNNNNATC 3 cut(s) 459, 966, 1473
BseBI CCWGG 8 cut(s) 983, 1318, 2445, 2526, 2643, 3035, 3490, 3586
BseCI ATCGAT 2 cut(s) 1037, 2499
BseJI GATNNNNATC 3 cut(s) 459, 966, 1473
BseMI GCAATG 1 cut(s) 2763
BseMII CTCAG 8 cut(s) 726, 1020, 1248, 1557, 1757, 2112, 2361, 2658
BseRI GAGGAG 1 cut(s) 752
BseSI GKGCMC 1 cut(s) 2401
BseXI GCAGC 7 cut(s) 1372, 1394, 1444, 1447, 1807, 2218, 2623
BsgI GTGCAG 4 cut(s) 710, 1371, 1383, 1401
BshNI GGYRCC 1 cut(s) 179
BshTI ACCGGT 1 cut(s) 1339
BshVI ATCGAT 2 cut(s) 1037, 2499
BsiHKAI GWGCWC 3 cut(s) 281, 1145, 3376
BsiHKCI CYCGRG 2 cut(s) 32, 1180
BsiSI CCGG 6 cut(s) 1123, 1340, 2169, 2270, 2680, 3392
BslFI GGGAC 5 cut(s) 107, 545, 2313, 2677, 2746
BsmFI GGGAC 5 cut(s) 107, 545, 2313, 2677, 2746
Bso31I GGTCTC 3 cut(s) 365, 501, 1079
BsoBI CYCGRG 2 cut(s) 32, 1180
Bsp1286I GDGCHC 4 cut(s) 281, 1145, 2401, 3376
Bsp1407I TGTACA 1 cut(s) 1799
Bsp19I CCATGG 1 cut(s) 3295
BspACI CCGC 2 cut(s) 2656, 3442
BspCNI CTCAG 8 cut(s) 727, 1019, 1249, 1558, 1756, 2113, 2362, 2659
BspDI ATCGAT 2 cut(s) 1037, 2499
BspHI TCATGA 2 cut(s) 595, 2017
BspLI GGNNCC 6 cut(s) 181, 741, 1056, 2796, 2913, 3312
BspMAI CTGCAG 1 cut(s) 3283
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 365, 501, 1079
BsrDI GCAATG 1 cut(s) 2763
BsrFI RCCGGY 2 cut(s) 1122, 1339
BsrGI TGTACA 1 cut(s) 1799
BssAI RCCGGY 2 cut(s) 1122, 1339
BssNI GRCGYC 1 cut(s) 3589
BssT1I CCWWGG 9 cut(s) 45, 54, 375, 1481, 2112, 2950, 3017, 3134, 3295
Bst2UI CCWGG 8 cut(s) 983, 1318, 2445, 2526, 2643, 3035, 3490, 3586
BstACI GRCGYC 1 cut(s) 3589
BstAPI GCANNNNNTGC 2 cut(s) 1648, 3278
BstAUI TGTACA 1 cut(s) 1799
BstBAI YACGTR 1 cut(s) 1811
BstDSI CCRYGG 1 cut(s) 3295
BstH2I RGCGCY 1 cut(s) 2855
BstHHI GCGC 3 cut(s) 2854, 3181, 3531
BstMWI GCNNNNNNNGC 5 cut(s) 1432, 1648, 2624, 3113, 3278
BstNI CCWGG 8 cut(s) 983, 1318, 2445, 2526, 2643, 3035, 3490, 3586
BstNSI RCATGY 2 cut(s) 1564, 2325
BstPAI GACNNNNGTC 1 cut(s) 1612
BstSCI CCNGG 9 cut(s) 981, 1316, 2167, 2443, 2524, 2641, 3033, 3488, 3584
BstSFI CTRYAG 3 cut(s) 1830, 2184, 3279
BstSLI GKGCMC 1 cut(s) 2401
BstV1I GCAGC 7 cut(s) 1372, 1394, 1444, 1447, 1807, 2218, 2623
BstV2I GAAGAC 3 cut(s) 684, 1598, 2861
BstX2I RGATCY 5 cut(s) 1468, 1489, 2794, 2911, 2967
BstXI CCANNNNNNTGG 2 cut(s) 1155, 3585
BstYI RGATCY 5 cut(s) 1468, 1489, 2794, 2911, 2967
Bsu15I ATCGAT 2 cut(s) 1037, 2499
Bsu36I CCTNAGG 1 cut(s) 3170
BsuI GTATCC 1 cut(s) 1215
BsuTUI ATCGAT 2 cut(s) 1037, 2499
BtgI CCRYGG 1 cut(s) 3295
BtgZI GCGATG 1 cut(s) 1838
BtrI CACGTC 1 cut(s) 2624
BtsI GCAGTG 4 cut(s) 209, 279, 1692, 3376
CaiI CAGNNNCTG 2 cut(s) 1505, 1769
CciI TCATGA 2 cut(s) 595, 2017
CfoI GCGC 3 cut(s) 2854, 3181, 3531
Cfr10I RCCGGY 2 cut(s) 1122, 1339
Cfr13I GGNCC 7 cut(s) 429, 1055, 1913, 2639, 2947, 3131, 3310
ClaI ATCGAT 2 cut(s) 1037, 2499
CseI GACGC 2 cut(s) 3524, 3578
CsiI ACCWGGT 1 cut(s) 1316
Csp6I GTAC 5 cut(s) 37, 207, 1800, 1933, 2634
CspAI ACCGGT 1 cut(s) 1339
CviQI GTAC 5 cut(s) 37, 207, 1800, 1933, 2634
EaeI YGGCCR 5 cut(s) 979, 1124, 2629, 2895, 2937
Ecl136II GAGCTC 1 cut(s) 1143
Eco130I CCWWGG 9 cut(s) 45, 54, 375, 1481, 2112, 2950, 3017, 3134, 3295
Eco147I AGGCCT 1 cut(s) 3016
Eco24I GRGCYC 1 cut(s) 1145
Eco31I GGTCTC 3 cut(s) 365, 501, 1079
Eco32I GATATC 2 cut(s) 349, 1041
Eco47I GGWCC 2 cut(s) 2947, 3310
Eco47III AGCGCT 1 cut(s) 2853
Eco53kI GAGCTC 1 cut(s) 1143
Eco57I CTGAAG 5 cut(s) 306, 1019, 1685, 1869, 2685
Eco81I CCTNAGG 1 cut(s) 3170
Eco88I CYCGRG 2 cut(s) 32, 1180
EcoICRI GAGCTC 1 cut(s) 1143
EcoO109I RGGNCCY 3 cut(s) 1055, 3131, 3310
EcoRII CCWGG 8 cut(s) 981, 1316, 2443, 2524, 2641, 3033, 3488, 3584
EcoRV GATATC 2 cut(s) 349, 1041
EcoT14I CCWWGG 9 cut(s) 45, 54, 375, 1481, 2112, 2950, 3017, 3134, 3295
EcoT22I ATGCAT 1 cut(s) 2233
EcoT38I GRGCYC 1 cut(s) 1145
ErhI CCWWGG 9 cut(s) 45, 54, 375, 1481, 2112, 2950, 3017, 3134, 3295
FalI AAGNNNNNCTT 2 cut(s) 2853, 2885
FaqI GGGAC 5 cut(s) 107, 545, 2313, 2677, 2746
FauI CCCGC 1 cut(s) 3435
FbaI TGATCA 2 cut(s) 967, 3040
Fnu4HI GCNGC 7 cut(s) 1383, 1386, 1433, 1436, 1821, 2207, 2612
FriOI GRGCYC 1 cut(s) 1145
Fsp4HI GCNGC 7 cut(s) 1383, 1386, 1433, 1436, 1821, 2207, 2612
FspBI CTAG 8 cut(s) 104, 2406, 2567, 2855, 3018, 3303, 3335, 3482
GlaI GCGC 3 cut(s) 2853, 3180, 3530
GluI GCNGC 7 cut(s) 1383, 1386, 1433, 1436, 1821, 2207, 2612
HaeII RGCGCY 1 cut(s) 2855
HapII CCGG 6 cut(s) 1123, 1340, 2169, 2270, 2680, 3392
HgaI GACGC 2 cut(s) 3524, 3578
HhaI GCGC 3 cut(s) 2854, 3181, 3531
Hin1I GRCGYC 1 cut(s) 3589
Hin6I GCGC 3 cut(s) 2852, 3179, 3529
HinP1I GCGC 3 cut(s) 2852, 3179, 3529
HincII GTYRAC 1 cut(s) 3460
HindII GTYRAC 1 cut(s) 3460
HinfI GANTC 9 cut(s) 263, 1016, 1357, 1375, 1454, 1978, 2570, 2989, 3343
HpaII CCGG 6 cut(s) 1123, 1340, 2169, 2270, 2680, 3392
Hpy166II GTNNAC 5 cut(s) 209, 1800, 1802, 2947, 3460
Hpy8I GTNNAC 5 cut(s) 209, 1800, 1802, 2947, 3460
HpyCH4IV ACGT 3 cut(s) 1810, 2623, 3544
HpyF10VI GCNNNNNNNGC 5 cut(s) 1432, 1648, 2624, 3113, 3278
HpySE526I ACGT 3 cut(s) 1810, 2623, 3544
Hsp92I GRCGYC 1 cut(s) 3589
HspAI GCGC 3 cut(s) 2852, 3179, 3529
KroI GCCGGC 1 cut(s) 1122
KroNI GCCGGC 1 cut(s) 1124
Ksp22I TGATCA 2 cut(s) 967, 3040
LmnI GCTCC 4 cut(s) 739, 1253, 1411, 2849
Lsp1109I GCAGC 7 cut(s) 1372, 1394, 1444, 1447, 1807, 2218, 2623
MabI ACCWGGT 1 cut(s) 1316
MaeI CTAG 8 cut(s) 104, 2406, 2567, 2855, 3018, 3303, 3335, 3482
MaeII ACGT 3 cut(s) 1810, 2623, 3544
MflI RGATCY 5 cut(s) 1468, 1489, 2794, 2911, 2967
MhlI GDGCHC 4 cut(s) 281, 1145, 2401, 3376
MlsI TGGCCA 2 cut(s) 981, 2939
MluNI TGGCCA 2 cut(s) 981, 2939
MlyI GAGTC 2 cut(s) 257, 1384
MmeI TCCRAC 3 cut(s) 654, 1522, 1861
Mox20I TGGCCA 2 cut(s) 981, 2939
Mph1103I ATGCAT 1 cut(s) 2233
MroNI GCCGGC 1 cut(s) 1122
MroXI GAANNNNTTC 1 cut(s) 1677
MscI TGGCCA 2 cut(s) 981, 2939
MseI TTAA 6 cut(s) 915, 1281, 1353, 1407, 2237, 2261
MslI CAYNNNNRTG 4 cut(s) 594, 600, 1781, 2984
Msp20I TGGCCA 2 cut(s) 981, 2939
MspA1I CMGCKG 2 cut(s) 1171, 1385
MspI CCGG 6 cut(s) 1123, 1340, 2169, 2270, 2680, 3392
MspR9I CCNGG 9 cut(s) 983, 1318, 2169, 2445, 2526, 2643, 3035, 3490, 3586
MvaI CCWGG 8 cut(s) 983, 1318, 2445, 2526, 2643, 3035, 3490, 3586
MwoI GCNNNNNNNGC 5 cut(s) 1432, 1648, 2624, 3113, 3278
NaeI GCCGGC 1 cut(s) 1124
NciI CCSGG 1 cut(s) 2169
NcoI CCATGG 1 cut(s) 3295
NgoMIV GCCGGC 1 cut(s) 1122
NlaIV GGNNCC 6 cut(s) 181, 741, 1056, 2796, 2913, 3312
NmeAIII GCCGAG 5 cut(s) 133, 208, 841, 2506, 2923
NmuCI GTSAC 8 cut(s) 1060, 1335, 1597, 1747, 2098, 2276, 2328, 3448
NsiI ATGCAT 1 cut(s) 2233
NspI RCATGY 2 cut(s) 1564, 2325
PaeI GCATGC 1 cut(s) 2325
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 595, 2017
PceI AGGCCT 1 cut(s) 3016
PcsI WCGNNNNNNNCGW 1 cut(s) 2629
PdiI GCCGGC 1 cut(s) 1124
PdmI GAANNNNTTC 1 cut(s) 1677
PfeI GAWTC 7 cut(s) 1016, 1357, 1454, 1978, 2570, 2989, 3343
PflMI CCANNNNNTGG 2 cut(s) 1505, 3415
PinAI ACCGGT 1 cut(s) 1339
PkrI GCNGC 7 cut(s) 1384, 1387, 1434, 1437, 1822, 2208, 2613
PleI GAGTC 2 cut(s) 257, 1383
PpsI GAGTC 2 cut(s) 257, 1383
Ppu21I YACGTR 1 cut(s) 1811
PpuMI RGGWCCY 1 cut(s) 3310
PshAI GACNNNNGTC 1 cut(s) 1612
PsiI TTATAA 2 cut(s) 762, 2550
Psp124BI GAGCTC 1 cut(s) 1145
Psp5II RGGWCCY 1 cut(s) 3310
Psp6I CCWGG 8 cut(s) 981, 1316, 2443, 2524, 2641, 3033, 3488, 3584
PspGI CCWGG 8 cut(s) 981, 1316, 2443, 2524, 2641, 3033, 3488, 3584
PspN4I GGNNCC 6 cut(s) 181, 741, 1056, 2796, 2913, 3312
PspPI GGNCC 7 cut(s) 429, 1055, 1913, 2639, 2947, 3131, 3310
PspPPI RGGWCCY 1 cut(s) 3310
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 3283
PstNI CAGNNNCTG 2 cut(s) 1505, 1769
PsuI RGATCY 5 cut(s) 1468, 1489, 2794, 2911, 2967
PvuII CAGCTG 2 cut(s) 1171, 1385
RsaI GTAC 5 cut(s) 38, 208, 1801, 1934, 2635
RsaNI GTAC 5 cut(s) 37, 207, 1800, 1933, 2634
RseI CAYNNNNRTG 4 cut(s) 594, 600, 1781, 2984
SacI GAGCTC 1 cut(s) 1145
SaqAI TTAA 6 cut(s) 915, 1281, 1353, 1407, 2237, 2261
SatI GCNGC 7 cut(s) 1383, 1386, 1433, 1436, 1821, 2207, 2612
Sau96I GGNCC 7 cut(s) 429, 1055, 1913, 2639, 2947, 3131, 3310
SchI GAGTC 2 cut(s) 257, 1384
ScrFI CCNGG 9 cut(s) 983, 1318, 2169, 2445, 2526, 2643, 3035, 3490, 3586
SduI GDGCHC 4 cut(s) 281, 1145, 2401, 3376
SexAI ACCWGGT 1 cut(s) 1316
SfcI CTRYAG 3 cut(s) 1830, 2184, 3279
Sfr274I CTCGAG 1 cut(s) 32
SgrAI CRCCGGYG 1 cut(s) 1339
SinI GGWCC 2 cut(s) 2947, 3310
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 4 cut(s) 594, 600, 1781, 2984
SmlI CTYRAG 2 cut(s) 32, 1268
SmoI CTYRAG 2 cut(s) 32, 1268
SphI GCATGC 1 cut(s) 2325
SseBI AGGCCT 1 cut(s) 3016
SsiI CCGC 2 cut(s) 2656, 3442
SspMI CTAG 8 cut(s) 104, 2406, 2567, 2855, 3018, 3303, 3335, 3482
SstI GAGCTC 1 cut(s) 1145
StuI AGGCCT 1 cut(s) 3016
StyD4I CCNGG 9 cut(s) 981, 1316, 2167, 2443, 2524, 2641, 3033, 3488, 3584
StyI CCWWGG 9 cut(s) 45, 54, 375, 1481, 2112, 2950, 3017, 3134, 3295
TaiI ACGT 3 cut(s) 1813, 2626, 3547
TaqI TCGA 5 cut(s) 33, 463, 1037, 2499, 3163
TaqII GACCGA 1 cut(s) 525
TatI WGTACW 2 cut(s) 1799, 1932
TfiI GAWTC 7 cut(s) 1016, 1357, 1454, 1978, 2570, 2989, 3343
Tru1I TTAA 6 cut(s) 915, 1281, 1353, 1407, 2237, 2261
Tru9I TTAA 6 cut(s) 915, 1281, 1353, 1407, 2237, 2261
TseFI GTSAC 8 cut(s) 1060, 1335, 1597, 1747, 2098, 2276, 2328, 3448
TseI GCWGC 7 cut(s) 1382, 1385, 1432, 1435, 1820, 2206, 2611
Tsp45I GTSAC 8 cut(s) 1060, 1335, 1597, 1747, 2098, 2276, 2328, 3448
TspGWI ACGGA 1 cut(s) 194
Van91I CCANNNNNTGG 2 cut(s) 1505, 3415
VpaK11BI GGWCC 2 cut(s) 2947, 3310
XapI RAATTY 4 cut(s) 152, 2253, 2712, 3250
XceI RCATGY 2 cut(s) 1564, 2325
XcmI CCANNNNNNNNNTGG 1 cut(s) 3472
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 3017
XmnI GAANNNNTTC 1 cut(s) 1677
XspI CTAG 8 cut(s) 104, 2406, 2567, 2855, 3018, 3303, 3335, 3482
Zsp2I ATGCAT 1 cut(s) 2233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.