Prupe.1G422500_v2.0.a1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000671
Physical Location & Seq
Reverse (-)
36465957 .. 36469722
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G422500.1

Sequence Viewer

Length: 3231 bp
ATGGCTGCTGCCACTGAAGATGTTTTCACGAAGCTCGACCACCTTTCCTTGCTCGCCAAGGTAGTTTCAGAGCTCGAAACTCATACGGGGTTGGGGGACGTTGTTCTTGCAGAGTTCATCACTGACTTGGGACGAAGCTGCGAGACTGTCGACGAGTTCAATTCCAAATTGCAGGAAAACGGTGCCCAGATGGCGGATTACTTTGTCCGTACACTCTTCGCCATCATTCACGCAATCAGTCGTCCGAAGCCTGAGAAGGTCTCGAAGAAAGACAGTGTCTTTGAGCGGCGTAGAAGTGATGAAGACAGGGAAAGAAGGCATGCGGATTTAGGCAATAAACGAAACAGAGATGGGTGTGAATTGTATACTGTTCATAAGGGTAGGGTGGCTAGAGTGATGGACAACGGTTGCTTTGTTCAGTTGAATGATCACGGAGGGAGGGAGGGTTTGGTTCATGTTTCCCAGATTTCGAATCGGAGAATTCGTAATGCCAAGGATGTGGTGAAGAGGGATCAGGAAGTTTATGTTAAGGTGGTTTCGGTTTCGGGTAGGAAGTTGAGTCTTTCGATGAGGGATGTTGATCAGCGAACTGGGAAGGATTTGCTTCCCTTGGAGAAGAAGGGCTCGGAGGGTGACGGTCTTTGGACAAACCCATTGGCGTCAAAGGATAGGCCTGTGACTAGGATTGGTATTTCTGGGATTAGGATTGAGGAAGAGGATGATGTTTTTGTCCCATCACGCCGCCGGCCATTGAAGAGAATGAGCTCGCCCGAGAAGTGGGAAGCCCAGCAGTTGATTGCCTCTGGTGTTTTGAGCGTTACAGAGTATCCAATGTATGATGAAGAAGAAGCAAATGGTATGCTTTATCAAGAAGAGGGAGCTGAGGAAGAGACTGAGGTTGAAACCAGGGAGGACAAGCCGGGGTTTTTGGAATATTCTTTGGACATGTCCCCTGTGAAGATCTTGAAGAATCCAGAAGGGTCATTGAGCCGCGCAGCTGCACTTCGATCTGCGCTAGCTAAGGAGGATAGAGAAGTGAGCAGGCTGGCAAAAGCCATGTTGAATTCTATCCCTAAGGATCTGAATCGTCCCTGGGAAGATCCAATGCCAGAGGCTGGTGAGAGGCAATTTGCACTAGAGCTAAGAGGTGTGGGTTTGTCAGGCTATGAAATGCCCGAGTGGAAGAAGGATGCTTTAGGGAAAACCATCAGTTTTGGGCAGAGGTCAAAGCTCTCAATTCAGGAACAGAGGAAGAGCTTGCCTGTCTACAAGCTGAAGGAACAACTGATTGAGGCGGTGCGTACTAATCCAGTGCTCGTCCTCATTGGTGAGACTGGTTCTGGTAAGACAACCCAGGTAACGCAGTACCTTGCAGAAGCGGGTTACACGACAAAGGGCAAGATTGGATGTACGCAGCCACGTAGGGTTGCTGCGATGTCTGTGGCCAAGAGGGTTGCTGAAGAGGTTGGTTGCCGTTTGGGAGAGGAAGTTGGCTATACAATTCGTTTCGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACCGACGGTATGCTTCTTAGGGAGATTATGATTGATGAGAACCTTTCTCAATATTCCGTGGTCATGCTTGACGAAGCTCACGAGAGGACGGTCGCTACAGACGTGCTTTTTGGTTTACTTAAGCAGCTTTTGGACCGGAGATCAGACTTTCGTTTGATTGCCACATCTGCTACACTGGATGCAGAGAGGTTTTCAGAATATTTCTGTGACTGTAAAATCTTCACCATCCCCGGGAGGCCTTTTCCCGTAGAGAAATTATACACCAAGCAGCCAGAAAGTGATTACCTTGACGCAGCTCTAATCACTGTCCTACAAATCCACTTAAAAGAACCAGAAGGTGACATCCTTCTCTTCTTGACTGGACAAGAAGAGATTGATTTTGCCTGCCAGACTCTATATGAGAGGATGAAAAGTCTTGGTAAAAATGTTCCTGAGTTGATTATTCTACCAGCATACAGCGCCCTTCCAAGTGAAATGCAGTCGAGGATATTTGATCCTGCTCCACCAGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCATCACTGACCATTGATGGGATATTTTATGTAATTGATCCGGGGTTTGCTAAACAAAATGTTTATAACCCAAAGCTGGGGCTCGACTCACTGATCATAACTCCAATTTCACAAGCGTCGGCTGAGCAACGAGCTGGGCGAGCAGGGCGTACAGGCCCTGGGAAATGTTACCGCCTCTACACTGAGAGTGCATACCGCAACGAGATGTCCCCAACTACAACTCCAGAAATCCAGAGGATAAATCTGGCGTCGACTGCACTTATGATGAAAGCTATGGGGATAAATGATCTCCTGAAATTTGATTTTATGGATTCTCCTCCACGCCAAGCACTCGTTTCTGCCATGGAACAACTGTACAATTTAGGAGCACTAGATGAGGAGGGGCTGCTGACCAGATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGAACCACCCCTGTCTAAGATGCTACTCGCCAGCGTGGACCTTGGATGCAGTGATGAGATGTTGACCATCATTGCAATGACTCAGACAGGCAATATGTTTCACAGGCCAAGGGAAAAACAAGCCCAGGCAGATCAGAAGAGAGCCAAGTTCTTTCAGCCAGAGGGAGACCACCTAACTTTACTTGCACTCTATGAGGCTTGGAAAGCAAGTAACTTTTCAGGGCCATGGTGTTTTAACAACTTCGTTCAGTCTCGATCCTTGAGGAGGGCACAGGATGTGAGAAAGCAGCTTCTGAGCATCATGGACAAGTACAAACTGGACGTTGTCAGCGCAGGAAAGCACTATACAAAGATCAGGAAGGCCATTACTGCAGGGTTCTTCTTTCACGCTGCTAGAAAGGACCCGCAGCAGGAGGGTGGCTGCTACAGAACCCTGGTGGAGAACCAGCAGGTTTATATCCATCCAAGCAGTGCGCTGTTCCAGAAACAACCAGATTGGGTCATCTACCATGAGCTGGTTATGACCTCAAAAGAGTACATGCGCGAGGTGACAGCCATAGACCCAAAATGGCTGGTGGAACTGGCACCAAGGTCCTTCAAATTTGCAGAGGCTAACAAGATGAGCAAGCGCAAGCGTCAAGAACGCATTGAGCCGCTATATGACAGATACAATGAGCCTAACTCTTGGCGCCTCAGTAGGCGTCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1077

Amino Acids

121.82

Weight (kDa)

7.22

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2160
Acc36I ACCTGC 1 cut(s) 2959
AccB1I GGYRCC 3 cut(s) 182, 3103, 3207
AccB7I CCANNNNNTGG 2 cut(s) 1115, 3034
AccBSI CCGCTC 1 cut(s) 286
AccI GTMKAC 4 cut(s) 150, 365, 1266, 2345
AccII CGCG 2 cut(s) 993, 3063
AclWI GGATC 6 cut(s) 519, 1086, 1094, 2036, 2126, 2769
AcoI YGGCCR 3 cut(s) 746, 1443, 2505
AcsI RAATTY 4 cut(s) 480, 1063, 2390, 3119
AcuI CTGAAG 3 cut(s) 36, 1295, 1479
AcyI GRCGYC 4 cut(s) 659, 2342, 3208, 3220
AfaI GTAC 9 cut(s) 211, 1303, 1367, 1411, 1544, 2245, 2450, 2831, 3056
AflII CTTAAG 1 cut(s) 1667
AflIII ACRYGT 1 cut(s) 945
AgsI TTSAA 7 cut(s) 160, 424, 754, 902, 967, 1063, 3118
AjiI CACGTC 1 cut(s) 1651
AjnI CCWGG 7 cut(s) 905, 1091, 1353, 2053, 2251, 2643, 2952
Alw21I GWGCWC 4 cut(s) 75, 767, 1317, 2464
Alw26I GTCTC 6 cut(s) 137, 265, 884, 1325, 2679, 2775
AlwI GGATC 6 cut(s) 519, 1086, 1094, 2036, 2126, 2769
AlwNI CAGNNNCTG 4 cut(s) 1115, 1532, 2252, 2812
Ama87I CYCGRG 3 cut(s) 770, 1175, 1778
AoxI GGCC 9 cut(s) 671, 746, 1443, 1784, 2248, 2505, 2624, 2741, 2880
ApoI RAATTY 4 cut(s) 480, 1063, 2390, 3119
ArsI GACNNNNNNTTYG 4 cut(s) 263, 295, 1640, 1672
AspLEI GCGC 8 cut(s) 995, 1015, 2009, 2852, 2995, 3063, 3150, 3210
AspS9I GGNCC 7 cut(s) 1523, 1681, 2249, 2557, 2741, 2920, 3111
AsuC2I CCSGG 4 cut(s) 921, 1779, 1780, 2136
AsuHPI GGTGA 7 cut(s) 514, 644, 1130, 1340, 1762, 1898, 3079
AsuII TTCGAA 1 cut(s) 470
AsuNHI GCTAGC 1 cut(s) 1015
AvaI CYCGRG 3 cut(s) 770, 1175, 1778
AvaII GGWCC 5 cut(s) 1523, 1681, 2557, 2920, 3111
AxyI CCTNAGG 1 cut(s) 1074
BaeGI GKGCMC 2 cut(s) 187, 2791
BalI TGGCCA 1 cut(s) 1445
BanI GGYRCC 3 cut(s) 182, 3103, 3207
BanII GRGCYC 4 cut(s) 75, 626, 767, 2178
BauI CACGAG 1 cut(s) 1628
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 4 cut(s) 75, 767, 1317, 2464
BbvCI CCTCAGC 2 cut(s) 882, 2091
BceAI ACGGC 1 cut(s) 1458
BciT130I CCWGG 7 cut(s) 907, 1093, 1355, 2055, 2253, 2645, 2954
BciVI GTATCC 1 cut(s) 837
BclI TGATCA 3 cut(s) 427, 580, 2187
BcnI CCSGG 4 cut(s) 921, 1779, 1780, 2136
BcoDI GTCTC 6 cut(s) 137, 265, 884, 1325, 2679, 2775
BfaI CTAG 6 cut(s) 390, 681, 1016, 1136, 2465, 2913
BfmI CTRYAG 3 cut(s) 1644, 2889, 2944
BfoI RGCGCY 2 cut(s) 2010, 3211
BfrI CTTAAG 1 cut(s) 1667
BfuAI ACCTGC 1 cut(s) 2959
BfuI GTATCC 1 cut(s) 837
BglI GCCNNNNNGGC 1 cut(s) 191
BglII AGATCT 1 cut(s) 960
BlpI GCTNAGC 1 cut(s) 2217
Bme18I GGWCC 5 cut(s) 1523, 1681, 2557, 2920, 3111
BmeT110I CYCGRG 3 cut(s) 770, 1175, 1778
BmgBI CACGTC 1 cut(s) 1651
BmgT120I GGNCC 7 cut(s) 1523, 1681, 2249, 2557, 2741, 2920, 3111
BmiI GGNNCC 5 cut(s) 184, 2523, 2922, 3105, 3209
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 6 cut(s) 1180, 1717, 2105, 2529, 2555, 2826
BmtI GCTAGC 1 cut(s) 1019
BmuI ACTGGG 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 309
BplI GAGNNNNNCTC 4 cut(s) 245, 277, 3185, 3217
BpmI CTGGAG 1 cut(s) 2301
Bpu10I CCTNAGC 3 cut(s) 882, 1020, 2091
Bpu1102I GCTNAGC 1 cut(s) 2217
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 2800
BpuMI CCSGG 4 cut(s) 921, 1779, 1780, 2136
BsaAI YACGTR 1 cut(s) 1421
BsaBI GATNNNNATC 2 cut(s) 579, 1083
BsaHI GRCGYC 4 cut(s) 659, 2342, 3208, 3220
BsaI GGTCTC 2 cut(s) 265, 2679
BsaWI WCCGGW 1 cut(s) 1683
BsaXI ACNNNNNCTCC 8 cut(s) 430, 460, 469, 499, 1473, 1503, 2299, 2329
Bse118I RCCGGY 1 cut(s) 744
Bse1I ACTGG 8 cut(s) 595, 1310, 1339, 1525, 1728, 1912, 2841, 3105
Bse21I CCTNAGG 1 cut(s) 1074
Bse3DI GCAATG 2 cut(s) 2589, 2601
Bse8I GATNNNNATC 2 cut(s) 579, 1083
BseBI CCWGG 7 cut(s) 907, 1093, 1355, 2055, 2253, 2645, 2954
BseJI GATNNNNATC 2 cut(s) 579, 1083
BseMI GCAATG 2 cut(s) 2589, 2601
BseNI ACTGG 8 cut(s) 595, 1310, 1339, 1525, 1728, 1912, 2841, 3105
BseRI GAGGAG 3 cut(s) 2400, 2486, 2797
BseSI GKGCMC 2 cut(s) 187, 2791
BseYI CCCAGC 3 cut(s) 786, 2170, 2228
BsgI GTGCAG 2 cut(s) 984, 2334
Bsh1236I CGCG 2 cut(s) 993, 3063
Bsh1285I CGRYCG 1 cut(s) 1641
BshFI GGCC 9 cut(s) 673, 748, 1445, 1786, 2250, 2507, 2626, 2743, 2882
BshNI GGYRCC 3 cut(s) 182, 3103, 3207
BsiEI CGRYCG 1 cut(s) 1641
BsiHKAI GWGCWC 4 cut(s) 75, 767, 1317, 2464
BsiHKCI CYCGRG 3 cut(s) 770, 1175, 1778
BsiSI CCGG 5 cut(s) 745, 920, 1684, 1779, 2135
BslFI GGGAC 6 cut(s) 110, 144, 716, 934, 1074, 2287
BsmAI GTCTC 6 cut(s) 137, 265, 884, 1325, 2679, 2775
BsmFI GGGAC 6 cut(s) 110, 144, 716, 934, 1074, 2287
BsnI GGCC 9 cut(s) 673, 748, 1445, 1786, 2250, 2507, 2626, 2743, 2882
Bso31I GGTCTC 2 cut(s) 265, 2679
BsoBI CYCGRG 3 cut(s) 770, 1175, 1778
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 8 cut(s) 75, 187, 626, 767, 1317, 2178, 2464, 2791
Bsp1407I TGTACA 1 cut(s) 2448
Bsp1720I GCTNAGC 1 cut(s) 2217
Bsp19I CCATGG 2 cut(s) 2436, 2744
BspANI GGCC 9 cut(s) 673, 748, 1445, 1786, 2250, 2507, 2626, 2743, 2882
BspFNI CGCG 2 cut(s) 993, 3063
BspLI GGNNCC 5 cut(s) 184, 2523, 2922, 3105, 3209
BspMAI CTGCAG 1 cut(s) 2893
BspMI ACCTGC 1 cut(s) 2959
BspOI GCTAGC 1 cut(s) 1019
BspPI GGATC 6 cut(s) 519, 1086, 1094, 2036, 2126, 2769
BspQI GCTCTTC 1 cut(s) 1247
BspT104I TTCGAA 1 cut(s) 470
BspT107I GGYRCC 3 cut(s) 182, 3103, 3207
BspTI CTTAAG 1 cut(s) 1667
BspTNI GGTCTC 2 cut(s) 265, 2679
BsrBI CCGCTC 1 cut(s) 286
BsrDI GCAATG 2 cut(s) 2589, 2601
BsrFI RCCGGY 1 cut(s) 744
BsrGI TGTACA 1 cut(s) 2448
BsrI ACTGG 8 cut(s) 595, 1310, 1339, 1525, 1728, 1912, 2841, 3105
BssAI RCCGGY 1 cut(s) 744
BssNAI GTATAC 1 cut(s) 366
BssNI GRCGYC 4 cut(s) 659, 2342, 3208, 3220
BssSI CACGAG 1 cut(s) 1628
BssT1I CCWWGG 8 cut(s) 57, 492, 609, 2436, 2560, 2627, 2744, 3107
Bst1107I GTATAC 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 1628
Bst2UI CCWGG 7 cut(s) 907, 1093, 1355, 2055, 2253, 2645, 2954
BstACI GRCGYC 4 cut(s) 659, 2342, 3208, 3220
BstAFI CTTAAG 1 cut(s) 1667
BstAUI TGTACA 1 cut(s) 2448
BstBAI YACGTR 1 cut(s) 1421
BstBI TTCGAA 1 cut(s) 470
BstDSI CCRYGG 3 cut(s) 1605, 2436, 2744
BstENI CCTNNNNNAGG 1 cut(s) 2782
BstFNI CGCG 2 cut(s) 993, 3063
BstH2I RGCGCY 2 cut(s) 2010, 3211
BstHHI GCGC 8 cut(s) 995, 1015, 2009, 2852, 2995, 3063, 3150, 3210
BstMAI GTCTC 6 cut(s) 137, 265, 884, 1325, 2679, 2775
BstMCI CGRYCG 1 cut(s) 1641
BstMWI GCNNNNNNNGC 8 cut(s) 191, 1715, 2006, 2234, 2239, 2348, 2723, 2888
BstNI CCWGG 7 cut(s) 907, 1093, 1355, 2055, 2253, 2645, 2954
BstNSI RCATGY 3 cut(s) 323, 949, 3061
BstSFI CTRYAG 3 cut(s) 1644, 2889, 2944
BstSLI GKGCMC 2 cut(s) 187, 2791
BstUI CGCG 2 cut(s) 993, 3063
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 3 cut(s) 960, 1078, 1099
BstXI CCANNNNNNTGG 1 cut(s) 499
BstYI RGATCY 3 cut(s) 960, 1078, 1099
BstZ17I GTATAC 1 cut(s) 366
Bsu36I CCTNAGG 1 cut(s) 1074
BsuI GTATCC 1 cut(s) 837
BsuRI GGCC 9 cut(s) 673, 748, 1445, 1786, 2250, 2507, 2626, 2743, 2882
BtgI CCRYGG 3 cut(s) 1605, 2436, 2744
BtgZI GCGATG 1 cut(s) 1448
BtrI CACGTC 1 cut(s) 1651
BtsI GCAGTG 2 cut(s) 2575, 2995
BveI ACCTGC 1 cut(s) 2959
CaiI CAGNNNCTG 4 cut(s) 1115, 1532, 2252, 2812
CfoI GCGC 8 cut(s) 995, 1015, 2009, 2852, 2995, 3063, 3150, 3210
Cfr10I RCCGGY 1 cut(s) 744
Cfr13I GGNCC 7 cut(s) 1523, 1681, 2249, 2557, 2741, 2920, 3111
Cfr9I CCCGGG 1 cut(s) 1778
CseI GACGC 6 cut(s) 648, 1847, 2199, 2331, 3143, 3209
Csp6I GTAC 9 cut(s) 210, 1302, 1366, 1410, 1543, 2244, 2449, 2830, 3055
CviQI GTAC 9 cut(s) 210, 1302, 1366, 1410, 1543, 2244, 2449, 2830, 3055
DinI GGCGCC 1 cut(s) 3209
EaeI YGGCCR 3 cut(s) 746, 1443, 2505
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 2 cut(s) 73, 765
Eco130I CCWWGG 8 cut(s) 57, 492, 609, 2436, 2560, 2627, 2744, 3107
Eco147I AGGCCT 2 cut(s) 673, 1786
Eco24I GRGCYC 4 cut(s) 75, 626, 767, 2178
Eco31I GGTCTC 2 cut(s) 265, 2679
Eco47I GGWCC 5 cut(s) 1523, 1681, 2557, 2920, 3111
Eco53kI GAGCTC 2 cut(s) 73, 765
Eco57I CTGAAG 3 cut(s) 36, 1295, 1479
Eco81I CCTNAGG 1 cut(s) 1074
Eco88I CYCGRG 3 cut(s) 770, 1175, 1778
EcoICRI GAGCTC 2 cut(s) 73, 765
EcoNI CCTNNNNNAGG 1 cut(s) 2782
EcoO109I RGGNCCY 3 cut(s) 2249, 2920, 3111
EcoRI GAATTC 2 cut(s) 480, 1063
EcoRII CCWGG 7 cut(s) 905, 1091, 1353, 2053, 2251, 2643, 2952
EcoT14I CCWWGG 8 cut(s) 57, 492, 609, 2436, 2560, 2627, 2744, 3107
EcoT38I GRGCYC 4 cut(s) 75, 626, 767, 2178
EgeI GGCGCC 1 cut(s) 3209
EheI GGCGCC 1 cut(s) 3209
ErhI CCWWGG 8 cut(s) 57, 492, 609, 2436, 2560, 2627, 2744, 3107
FaqI GGGAC 6 cut(s) 110, 144, 716, 934, 1074, 2287
FauI CCCGC 2 cut(s) 1372, 2931
FbaI TGATCA 3 cut(s) 427, 580, 2187
FblI GTMKAC 4 cut(s) 150, 365, 1266, 2345
FriOI GRGCYC 4 cut(s) 75, 626, 767, 2178
FspBI CTAG 6 cut(s) 390, 681, 1016, 1136, 2465, 2913
GlaI GCGC 8 cut(s) 994, 1014, 2008, 2851, 2994, 3062, 3149, 3209
GsaI CCCAGC 3 cut(s) 790, 2174, 2232
GsuI CTGGAG 1 cut(s) 2301
HaeII RGCGCY 2 cut(s) 2010, 3211
HaeIII GGCC 9 cut(s) 673, 748, 1445, 1786, 2250, 2507, 2626, 2743, 2882
HapII CCGG 5 cut(s) 745, 920, 1684, 1779, 2135
HgaI GACGC 6 cut(s) 648, 1847, 2199, 2331, 3143, 3209
HhaI GCGC 8 cut(s) 995, 1015, 2009, 2852, 2995, 3063, 3150, 3210
Hin1I GRCGYC 4 cut(s) 659, 2342, 3208, 3220
Hin6I GCGC 8 cut(s) 993, 1013, 2007, 2850, 2993, 3061, 3148, 3208
HinP1I GCGC 8 cut(s) 993, 1013, 2007, 2850, 2993, 3061, 3148, 3208
HincII GTYRAC 3 cut(s) 151, 2346, 2583
HindII GTYRAC 3 cut(s) 151, 2346, 2583
HinfI GANTC 8 cut(s) 472, 559, 970, 1084, 1939, 2180, 2405, 2599
HpaII CCGG 5 cut(s) 745, 920, 1684, 1779, 2135
HphI GGTGA 7 cut(s) 514, 644, 1130, 1340, 1762, 1898, 3079
Hpy166II GTNNAC 8 cut(s) 151, 212, 366, 1267, 1664, 2346, 2557, 2583
Hpy8I GTNNAC 8 cut(s) 151, 212, 366, 1267, 1664, 2346, 2557, 2583
Hpy99I CGWCG 5 cut(s) 155, 1556, 2215, 2347, 3225
HpyCH4IV ACGT 4 cut(s) 99, 1420, 1650, 2841
HpyF10VI GCNNNNNNNGC 8 cut(s) 191, 1715, 2006, 2234, 2239, 2348, 2723, 2888
HpySE526I ACGT 4 cut(s) 99, 1420, 1650, 2841
Hsp92I GRCGYC 4 cut(s) 659, 2342, 3208, 3220
HspAI GCGC 8 cut(s) 993, 1013, 2007, 2850, 2993, 3061, 3148, 3208
KasI GGCGCC 1 cut(s) 3207
KroI GCCGGC 1 cut(s) 744
KroNI GCCGGC 1 cut(s) 746
Ksp22I TGATCA 3 cut(s) 427, 580, 2187
LguI GCTCTTC 1 cut(s) 1247
LmnI GCTCC 3 cut(s) 878, 2053, 2459
LweI GCATC 6 cut(s) 1180, 1717, 2105, 2529, 2555, 2826
MaeI CTAG 6 cut(s) 390, 681, 1016, 1136, 2465, 2913
MaeII ACGT 4 cut(s) 99, 1420, 1650, 2841
MbiI CCGCTC 1 cut(s) 286
MflI RGATCY 3 cut(s) 960, 1078, 1099
MhlI GDGCHC 8 cut(s) 75, 187, 626, 767, 1317, 2178, 2464, 2791
MlsI TGGCCA 1 cut(s) 1445
MluNI TGGCCA 1 cut(s) 1445
Mly113I GGCGCC 1 cut(s) 3208
MlyI GAGTC 4 cut(s) 568, 1933, 2174, 2593
Mox20I TGGCCA 1 cut(s) 1445
MroNI GCCGGC 1 cut(s) 744
MscI TGGCCA 1 cut(s) 1445
MseI TTAA 4 cut(s) 528, 1668, 1871, 2754
MslI CAYNNNNRTG 1 cut(s) 2594
Msp20I TGGCCA 1 cut(s) 1445
MspA1I CMGCKG 1 cut(s) 998
MspCI CTTAAG 1 cut(s) 1667
MspI CCGG 5 cut(s) 745, 920, 1684, 1779, 2135
MvaI CCWGG 7 cut(s) 907, 1093, 1355, 2055, 2253, 2645, 2954
MvnI CGCG 2 cut(s) 993, 3063
MwoI GCNNNNNNNGC 8 cut(s) 191, 1715, 2006, 2234, 2239, 2348, 2723, 2888
NaeI GCCGGC 1 cut(s) 746
NarI GGCGCC 1 cut(s) 3208
NciI CCSGG 4 cut(s) 921, 1779, 1780, 2136
NcoI CCATGG 2 cut(s) 2436, 2744
NgoMIV GCCGGC 1 cut(s) 744
NheI GCTAGC 1 cut(s) 1015
NlaIV GGNNCC 5 cut(s) 184, 2523, 2922, 3105, 3209
NmeAIII GCCGAG 1 cut(s) 2533
NmuCI GTSAC 5 cut(s) 632, 676, 1754, 1886, 3067
NspI RCATGY 3 cut(s) 323, 949, 3061
NspV TTCGAA 1 cut(s) 470
PaeI GCATGC 1 cut(s) 323
PasI CCCWGGG 2 cut(s) 1092, 2252
PceI AGGCCT 2 cut(s) 673, 1786
PciI ACATGT 1 cut(s) 945
PciSI GCTCTTC 1 cut(s) 1247
PcsI WCGNNNNNNNCGW 4 cut(s) 481, 1626, 1647, 2230
PdiI GCCGGC 1 cut(s) 746
PfeI GAWTC 4 cut(s) 472, 970, 1084, 2405
PflFI GACNNNGTC 2 cut(s) 275, 2843
PflMI CCANNNNNTGG 2 cut(s) 1115, 3034
PleI GAGTC 4 cut(s) 567, 1933, 2174, 2593
PluTI GGCGCC 1 cut(s) 3211
PpsI GAGTC 4 cut(s) 567, 1933, 2174, 2593
Ppu21I YACGTR 1 cut(s) 1421
PpuMI RGGWCCY 2 cut(s) 2920, 3111
PscI ACATGT 1 cut(s) 945
PsiI TTATAA 1 cut(s) 2160
Psp124BI GAGCTC 2 cut(s) 75, 767
Psp5II RGGWCCY 2 cut(s) 2920, 3111
Psp6I CCWGG 7 cut(s) 905, 1091, 1353, 2053, 2251, 2643, 2952
PspFI CCCAGC 3 cut(s) 786, 2170, 2228
PspGI CCWGG 7 cut(s) 905, 1091, 1353, 2053, 2251, 2643, 2952
PspN4I GGNNCC 5 cut(s) 184, 2523, 2922, 3105, 3209
PspPI GGNCC 7 cut(s) 1523, 1681, 2249, 2557, 2741, 2920, 3111
PspPPI RGGWCCY 2 cut(s) 2920, 3111
PstI CTGCAG 1 cut(s) 2893
PstNI CAGNNNCTG 4 cut(s) 1115, 1532, 2252, 2812
PsuI RGATCY 3 cut(s) 960, 1078, 1099
PsyI GACNNNGTC 2 cut(s) 275, 2843
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 9 cut(s) 211, 1303, 1367, 1411, 1544, 2245, 2450, 2831, 3056
RsaNI GTAC 9 cut(s) 210, 1302, 1366, 1410, 1543, 2244, 2449, 2830, 3055
RseI CAYNNNNRTG 1 cut(s) 2594
SacI GAGCTC 2 cut(s) 75, 767
SalI GTCGAC 2 cut(s) 149, 2344
SapI GCTCTTC 1 cut(s) 1247
SaqAI TTAA 4 cut(s) 528, 1668, 1871, 2754
Sau96I GGNCC 7 cut(s) 1523, 1681, 2249, 2557, 2741, 2920, 3111
SchI GAGTC 4 cut(s) 568, 1933, 2174, 2593
SduI GDGCHC 8 cut(s) 75, 187, 626, 767, 1317, 2178, 2464, 2791
SfaNI GCATC 6 cut(s) 1180, 1717, 2105, 2529, 2555, 2826
SfcI CTRYAG 3 cut(s) 1644, 2889, 2944
SfoI GGCGCC 1 cut(s) 3209
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 5 cut(s) 1523, 1681, 2557, 2920, 3111
SmaI CCCGGG 1 cut(s) 1780
SmiMI CAYNNNNRTG 1 cut(s) 2594
SmlI CTYRAG 2 cut(s) 1667, 2779
SmoI CTYRAG 2 cut(s) 1667, 2779
SphI GCATGC 1 cut(s) 323
SseBI AGGCCT 2 cut(s) 673, 1786
SspDI GGCGCC 1 cut(s) 3207
SspI AATATT 4 cut(s) 935, 1601, 1748, 2086
SspMI CTAG 6 cut(s) 390, 681, 1016, 1136, 2465, 2913
SstI GAGCTC 2 cut(s) 75, 767
StuI AGGCCT 2 cut(s) 673, 1786
StyI CCWWGG 8 cut(s) 57, 492, 609, 2436, 2560, 2627, 2744, 3107
TaiI ACGT 4 cut(s) 102, 1423, 1653, 2844
TaqII GACCGA 1 cut(s) 1565
TatI WGTACW 4 cut(s) 1542, 2448, 2829, 3054
TauI GCSGC 4 cut(s) 289, 744, 993, 3175
TfiI GAWTC 4 cut(s) 472, 970, 1084, 2405
Tru1I TTAA 4 cut(s) 528, 1668, 1871, 2754
Tru9I TTAA 4 cut(s) 528, 1668, 1871, 2754
TseFI GTSAC 5 cut(s) 632, 676, 1754, 1886, 3067
Tsp45I GTSAC 5 cut(s) 632, 676, 1754, 1886, 3067
TspDTI ATGAA 8 cut(s) 106, 315, 362, 443, 855, 1182, 1970, 2375
TspGWI ACGGA 3 cut(s) 197, 447, 1594
TspMI CCCGGG 1 cut(s) 1778
Tth111I GACNNNGTC 2 cut(s) 275, 2843
Van91I CCANNNNNTGG 2 cut(s) 1115, 3034
Vha464I CTTAAG 1 cut(s) 1667
VpaK11BI GGWCC 5 cut(s) 1523, 1681, 2557, 2920, 3111
XagI CCTNNNNNAGG 1 cut(s) 2782
XapI RAATTY 4 cut(s) 480, 1063, 2390, 3119
XceI RCATGY 3 cut(s) 323, 949, 3061
XmaI CCCGGG 1 cut(s) 1778
XmiI GTMKAC 4 cut(s) 150, 365, 1266, 2345
XspI CTAG 6 cut(s) 390, 681, 1016, 1136, 2465, 2913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.