FvH4_4g07500

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000671
Physical Location & Seq
Reverse (-)
6869741 .. 6877256
7516 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07500.t2

Sequence Viewer

Length: 3570 bp
ATGGCTGTCATTGACGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCTAAAGTCTGTTCAGAGCTGGAAACACATCTAGGCTTTGGGGATAAAGTTTTAGCTGAGTTCATAACTGAGATGGGACGAAACTGTGGGAGTGTGGATGAATTTGATGCCAAATTAAAGGAAAATGGTGCTGAGATGCCTGATTATTTTGTCCGCACACTCCTCACTATCATTCACGCAATTCTTCCTCCAAAGCCAAAGTCTGAGGATGACTCGATGATAGAGAGTAATACTGAAGGTAAGAAGACCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGATAAATGATCTCCAGAAGGAAATTGAGATGGAGACCAAAGAAAAGAAAAAAGACCGAGAAGAGTGGGGTGATGAGCTTGAAGAAGATAGGCGTAGAGGTAGAGGTAGAGATAGAGACAGATATGGGGAAAGAGAACGAGACAGAGATAGGGATAGCGATAGAAGAGAGAGGGGCAGGGATATGTATGACAGAGGTGAGAGGCGTAGGGATACTCATCATGATTATGATAAGGGAAGTCATACCGACCGATCCAATAAGCCCAGGAGGGATGGGTATGAAGAGGATGTAGATGGTAGACAAGGTAGTGGTAACAGAAGAGGAGGTGGGGATCAGCGGAGTGGTCACAATTCTGATGAGCCCGAATTGTATCAGGTTTATAAGGGTAGGGTATCAAAAGTCATGGACACGGGTTGCTTTGTTCAGTTTAGTGACTTTAGGGGAAAGGAGGGTTTGGTTCATGTTTCACAAATGGCAACTCGGCGTATTGGTAACGCTAAAGATGTGGTGAAGAGGGATCAAGAGGTCTATGTTAAGGTGATTTCGGTTTCTGGTCAGAAATTGAGCCTTTCAATGAGGGATGTTGATCAGCATACCGGAAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCTTAGGACGAACCCTGCGGTATCAAAGGATGAAAGGCCTGTGACAAGGACTGGTCTTTCTGGGATTAGAATTGTGGAAGAGAACACTGGTGTCTCTTCTCGTCGGCCATTAAAGAGAATGAGCTCGCCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAATATCCAATGTATGATGAGGAAACAGAGGGGATGCTTTATGAAGAAGAGGGCGCTGAGGAAGAACTTGAGATTGAGCTTAATGAAGACGAGCCAGCCTTTTTGCAAGGGCAAAGCAGGTATTCTGTAGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAGGGGTCCTTAAGCCGTGCTGCGGCACTCCAATCTGCACTTATTAAAGAGCGCAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCGCAGGAGCTTAGAGGTGTTGGTTTGTCAGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCAGTTTTGGGCAGAGATCAAAGCTCTCACTTCAGGAACAGAGGCAGAGCTTACCCATCTACAAGCTCAAGAAAGAATTGGTTCAGGCAGTGCATGATAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCGGGAAAGACAACCCAGGTAACACAATATCTTGCAGAAGCAGGTTACACGACAATGGGTAAGATCGGGTGTACACAACCACGTAGGGTGGCTGCAACATCTGTAGCCAAGAGAGTGGCTGAAGAATTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGCCCTGAAACCGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATTGATGAGAATCTCTCTCAGTATTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTGTTTGGATTATTGAAGAAACTTATCAAGCGGAGACCAGACCTACGTTTGATCGTCACATCTGCTACCTTAGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATACCGGGTAGAACTTTCCCTGTAGAGATACTCTACACAAAGCAGCCAGAAAGTGATTATCTTGATGCCTCTCTAATCACTGTTCTACAGATTCACTTGACGGAACCAGAAGGGGACATCCTTCTCTTCTTGACAGGACAGGAAGAAATTGATTTCTCATGCCAGTCACTTTTTGAGAGGATGAAAGGTCTAGGTAAAAATGTGCCTGAGTTAATTATCTTACCAGTTTATAGTGCCCTTCCTAGTGAAATGCAGTCCAGGATATTTGATCCAGCTCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCCGAGGCATCACTAACCATCGATGGGATATTTTATGTAATTGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTGGATTCACTGGTCATAACCCCAATTTCACAAGCATCAGCCAAGCAAAGGGCTGGGCGTGCTGGGCGTACTGGGCCTGGGAAATGTTACCGCCTCTACACTGAGAGTGCATACCGCAATGAGATGTCCCCCACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGTTAACTACGCTTACCATGAAAGCAATGGGGATCAATGATCTGCTTTCTTTTGATTTTATGGATCCTCCATCACCCCAAGCACTTATTTCTGCTATGGAACAACTGTACAGTTTAGGAGCACTCGATGAGGAGGGGCTTCTCACCAAACTGGGTAGGAAAATGGCTGAGTTTCCTATTGATCCGCCACTATCTAAGATGCTACTGGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATGATTGCCATGATTCAAACAGGCAATATATTTTATAGGCCTAGGGAGAAACAAGCCCAGGCTGATCAGAAGAGAGCCAAATTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTGTATGAGGCTTGGAAAGCTAAGAACTTTTCCGGGCCATGGTGTTTTGAGAACTTCGTTCAGTCTCGGTCCCTTAGGAGAGCTCAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAGCTGGATGTTGTGAGTGCTGGAAAGAATTTTACACAGATCAGGAAGGCAATTACAGCAGGATTTTTCTTCCATGGTGCTAGAAAGGACCCTCAGGAGGGGTATAGAACCTTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCGCTTTTTCAGAGACAGCCAGATTGGATCATCTACCATGAGTTGGTTATGACTACGAAGGAGTATATGCGTGAGGTAACAGTTGTTGATCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAAGTGGCAGACCCTACTAAGATGAGTAAGCGTAAGCGTCAAGAGCGTGTTGAACCGCTATATGACAGATACCATGAACCGAACTCTTGGCGTCTTAGTAAACGACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1190

Amino Acids

135.42

Weight (kDa)

6.2

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 234 - 304 1.3e-11 S1 RNA binding domain
DEAD PF00270 543 - 692 3.4e-07 DEAD/DEAH box helicase
AAA_22 PF13401 551 - 676 1.6e-06 AAA domain
Helicase_C PF00271 736 - 866 8.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 928 - 956 1.3e-10 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 957 - 1016 3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1074 - 1150 8.3e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 717, 2508
Acc16I TGCGCA 1 cut(s) 1482
Acc36I ACCTGC 2 cut(s) 1266, 1718
AccB7I CCANNNNNTGG 2 cut(s) 1463, 3373
AccI GTMKAC 1 cut(s) 634
AciI CCGC 9 cut(s) 205, 673, 995, 1343, 2032, 2614, 2638, 2873, 3512
AcoI YGGCCR 2 cut(s) 1082, 2895
AcsI RAATTY 5 cut(s) 152, 1811, 2670, 3014, 3208
AcuI CTGAAG 4 cut(s) 306, 1571, 1827, 2643
AcyI GRCGYC 1 cut(s) 3547
AfaI GTAC 5 cut(s) 38, 1759, 1892, 2593, 2798
AflII CTTAAG 1 cut(s) 1330
AjiI CACGTC 1 cut(s) 3563
AjnI CCWGG 8 cut(s) 599, 1701, 2378, 2401, 2482, 2599, 2991, 3446
Alw21I GWGCWC 4 cut(s) 965, 1103, 2812, 3139
AlwNI CAGNNNCTG 2 cut(s) 1463, 2524
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 7 cut(s) 1013, 1082, 1870, 2597, 2895, 2972, 3089
ApeKI GCWGC 5 cut(s) 1340, 1390, 1393, 1778, 2164
ApoI RAATTY 5 cut(s) 152, 1811, 2670, 3014, 3208
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
Asp700I GAANNNNTTC 1 cut(s) 1635
AspA2I CCTAGG 1 cut(s) 2975
AspLEI GCGC 4 cut(s) 1214, 1374, 1483, 3334
AspS9I GGNCC 7 cut(s) 1326, 1871, 2597, 2905, 3089, 3123, 3268
AsuC2I CCSGG 2 cut(s) 2127, 3088
AsuHPI GGTGA 9 cut(s) 419, 545, 856, 886, 1287, 1478, 1688, 2754, 2824
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 4 cut(s) 1326, 2905, 3123, 3268
AvrII CCTAGG 1 cut(s) 2975
AxyI CCTNAGG 3 cut(s) 1138, 3128, 3273
BaeGI GKGCMC 1 cut(s) 2359
BalI TGGCCA 1 cut(s) 2897
BamHI GGATCC 1 cut(s) 2752
BanII GRGCYC 4 cut(s) 699, 965, 1103, 3139
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 3 cut(s) 302, 1251, 1556
Bbv12I GWGCWC 4 cut(s) 965, 1103, 2812, 3139
BbvCI CCTCAGC 1 cut(s) 1215
BbvI GCAGC 5 cut(s) 1327, 1402, 1405, 1765, 2176
BceAI ACGGC 1 cut(s) 1320
BciT130I CCWGG 8 cut(s) 601, 1703, 2380, 2403, 2484, 2601, 2993, 3448
BciVI GTATCC 1 cut(s) 541
BclI TGATCA 2 cut(s) 922, 2998
BcnI CCSGG 2 cut(s) 2127, 3088
BfaI CTAG 6 cut(s) 83, 2312, 2364, 2976, 3261, 3440
BfmI CTRYAG 4 cut(s) 1284, 1788, 2142, 2207
BfoI RGCGCY 1 cut(s) 1215
BfrI CTTAAG 1 cut(s) 1330
BfuAI ACCTGC 2 cut(s) 1266, 1718
BfuI GTATCC 1 cut(s) 541
BglII AGATCT 1 cut(s) 2925
BisI GCNGC 6 cut(s) 1341, 1344, 1391, 1394, 1779, 2165
BlnI CCTAGG 1 cut(s) 2975
BlsI GCNGC 6 cut(s) 1342, 1345, 1392, 1395, 1780, 2166
Bme18I GGWCC 4 cut(s) 1326, 2905, 3123, 3268
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 3563
BmgT120I GGNCC 7 cut(s) 1326, 1871, 2597, 2905, 3089, 3123, 3268
BmiI GGNNCC 5 cut(s) 1327, 2226, 2754, 3125, 3270
BmrI ACTGGG 2 cut(s) 2604, 2849
BmuI ACTGGG 2 cut(s) 2604, 2849
BpiI GAAGAC 3 cut(s) 302, 1251, 1556
BplI GAGNNNNNCTC 4 cut(s) 248, 280, 1925, 1957
BpmI CTGGAG 1 cut(s) 335
Bpu10I CCTNAGC 2 cut(s) 1215, 3138
BpuEI CTTGAG 2 cut(s) 1247, 1607
BpuMI CCSGG 2 cut(s) 2127, 3088
Bsa29I ATCGAT 1 cut(s) 2457
BsaAI YACGTR 1 cut(s) 1769
BsaBI GATNNNNATC 4 cut(s) 552, 921, 1431, 1935
BsaHI GRCGYC 1 cut(s) 3547
BsaI GGTCTC 3 cut(s) 365, 2029, 3027
BsaWI WCCGGW 1 cut(s) 932
Bse21I CCTNAGG 3 cut(s) 1138, 3128, 3273
Bse3DI GCAATG 2 cut(s) 2647, 2721
Bse8I GATNNNNATC 4 cut(s) 552, 921, 1431, 1935
BseBI CCWGG 8 cut(s) 601, 1703, 2380, 2403, 2484, 2601, 2993, 3448
BseCI ATCGAT 1 cut(s) 2457
BseJI GATNNNNATC 4 cut(s) 552, 921, 1431, 1935
BseMI GCAATG 2 cut(s) 2647, 2721
BseRI GAGGAG 3 cut(s) 203, 672, 2834
BseSI GKGCMC 1 cut(s) 2359
BseXI GCAGC 5 cut(s) 1327, 1402, 1405, 1765, 2176
BseYI CCCAGC 2 cut(s) 2576, 2585
BsgI GTGCAG 1 cut(s) 1341
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 7 cut(s) 1015, 1084, 1872, 2599, 2897, 2974, 3091
BshVI ATCGAT 1 cut(s) 2457
BsiEI CGRYCG 1 cut(s) 586
BsiHKAI GWGCWC 4 cut(s) 965, 1103, 2812, 3139
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 4 cut(s) 933, 1106, 2126, 3087
BslFI GGGAC 4 cut(s) 141, 2249, 2635, 3109
BsmFI GGGAC 4 cut(s) 141, 2249, 2635, 3109
BsnI GGCC 7 cut(s) 1015, 1084, 1872, 2599, 2897, 2974, 3091
Bso31I GGTCTC 3 cut(s) 365, 2029, 3027
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 6 cut(s) 699, 965, 1103, 2359, 2812, 3139
Bsp1407I TGTACA 2 cut(s) 1757, 2796
Bsp19I CCATGG 2 cut(s) 3092, 3253
BspACI CCGC 9 cut(s) 205, 673, 995, 1343, 2032, 2614, 2638, 2873, 3512
BspANI GGCC 7 cut(s) 1015, 1084, 1872, 2599, 2897, 2974, 3091
BspDI ATCGAT 1 cut(s) 2457
BspHI TCATGA 2 cut(s) 556, 1975
BspLI GGNNCC 5 cut(s) 1327, 2226, 2754, 3125, 3270
BspMI ACCTGC 2 cut(s) 1266, 1718
BspQI GCTCTTC 1 cut(s) 953
BspTI CTTAAG 1 cut(s) 1330
BspTNI GGTCTC 3 cut(s) 365, 2029, 3027
BsrDI GCAATG 2 cut(s) 2647, 2721
BsrGI TGTACA 2 cut(s) 1757, 2796
BssNI GRCGYC 1 cut(s) 3547
BssT1I CCWWGG 6 cut(s) 45, 1439, 2908, 2975, 3092, 3253
Bst2UI CCWGG 8 cut(s) 601, 1703, 2380, 2403, 2484, 2601, 2993, 3448
Bst4CI ACNGT 9 cut(s) 137, 1128, 1555, 1882, 2108, 2203, 2796, 2801, 3412
BstACI GRCGYC 1 cut(s) 3547
BstAFI CTTAAG 1 cut(s) 1330
BstAUI TGTACA 2 cut(s) 1757, 2796
BstBAI YACGTR 1 cut(s) 1769
BstC8I GCNNGC 3 cut(s) 1103, 1254, 2583
BstDSI CCRYGG 2 cut(s) 3092, 3253
BstH2I RGCGCY 1 cut(s) 1215
BstHHI GCGC 4 cut(s) 1214, 1374, 1483, 3334
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 8 cut(s) 1390, 1480, 2582, 2587, 2596, 3071, 3236, 3499
BstNI CCWGG 8 cut(s) 601, 1703, 2380, 2403, 2484, 2601, 2993, 3448
BstNSI RCATGY 1 cut(s) 1522
BstSFI CTRYAG 4 cut(s) 1284, 1788, 2142, 2207
BstSLI GKGCMC 1 cut(s) 2359
BstV1I GCAGC 5 cut(s) 1327, 1402, 1405, 1765, 2176
BstV2I GAAGAC 3 cut(s) 302, 1251, 1556
BstX2I RGATCY 4 cut(s) 1426, 1447, 2752, 2925
BstXI CCANNNNNNTGG 1 cut(s) 1801
BstYI RGATCY 4 cut(s) 1426, 1447, 2752, 2925
Bsu15I ATCGAT 1 cut(s) 2457
Bsu36I CCTNAGG 3 cut(s) 1138, 3128, 3273
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 7 cut(s) 1015, 1084, 1872, 2599, 2897, 2974, 3091
BsuTUI ATCGAT 1 cut(s) 2457
BtgI CCRYGG 2 cut(s) 3092, 3253
BtrI CACGTC 1 cut(s) 3563
BtsI GCAGTG 4 cut(s) 1650, 2923, 3060, 3334
BveI ACCTGC 2 cut(s) 1266, 1718
Cac8I GCNNGC 3 cut(s) 1103, 1254, 2583
CaiI CAGNNNCTG 2 cut(s) 1463, 2524
CciI TCATGA 2 cut(s) 556, 1975
CfoI GCGC 4 cut(s) 1214, 1374, 1483, 3334
Cfr13I GGNCC 7 cut(s) 1326, 1871, 2597, 2905, 3089, 3123, 3268
ClaI ATCGAT 1 cut(s) 2457
CseI GACGC 2 cut(s) 3482, 3536
Csp6I GTAC 5 cut(s) 37, 1758, 1891, 2592, 2797
CviQI GTAC 5 cut(s) 37, 1758, 1891, 2592, 2797
EaeI YGGCCR 2 cut(s) 1082, 2895
EciI GGCGGA 1 cut(s) 2862
Ecl136II GAGCTC 3 cut(s) 963, 1101, 3137
Eco130I CCWWGG 6 cut(s) 45, 1439, 2908, 2975, 3092, 3253
Eco147I AGGCCT 2 cut(s) 1015, 2974
Eco24I GRGCYC 4 cut(s) 699, 965, 1103, 3139
Eco31I GGTCTC 3 cut(s) 365, 2029, 3027
Eco47I GGWCC 4 cut(s) 1326, 2905, 3123, 3268
Eco53kI GAGCTC 3 cut(s) 963, 1101, 3137
Eco57I CTGAAG 4 cut(s) 306, 1571, 1827, 2643
Eco81I CCTNAGG 3 cut(s) 1138, 3128, 3273
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 3 cut(s) 963, 1101, 3137
EcoO109I RGGNCCY 2 cut(s) 1326, 3268
EcoRII CCWGG 8 cut(s) 599, 1701, 2378, 2401, 2482, 2599, 2991, 3446
EcoT14I CCWWGG 6 cut(s) 45, 1439, 2908, 2975, 3092, 3253
EcoT38I GRGCYC 4 cut(s) 699, 965, 1103, 3139
ErhI CCWWGG 6 cut(s) 45, 1439, 2908, 2975, 3092, 3253
FaqI GGGAC 4 cut(s) 141, 2249, 2635, 3109
FbaI TGATCA 2 cut(s) 922, 2998
FblI GTMKAC 1 cut(s) 634
Fnu4HI GCNGC 6 cut(s) 1341, 1344, 1391, 1394, 1779, 2165
FriOI GRGCYC 4 cut(s) 699, 965, 1103, 3139
Fsp4HI GCNGC 6 cut(s) 1341, 1344, 1391, 1394, 1779, 2165
FspBI CTAG 6 cut(s) 83, 2312, 2364, 2976, 3261, 3440
FspI TGCGCA 1 cut(s) 1482
GlaI GCGC 4 cut(s) 1213, 1373, 1482, 3333
GluI GCNGC 6 cut(s) 1341, 1344, 1391, 1394, 1779, 2165
GsaI CCCAGC 2 cut(s) 2580, 2589
GsuI CTGGAG 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 1215
HaeIII GGCC 7 cut(s) 1015, 1084, 1872, 2599, 2897, 2974, 3091
HapII CCGG 4 cut(s) 933, 1106, 2126, 3087
HgaI GACGC 2 cut(s) 3482, 3536
HhaI GCGC 4 cut(s) 1214, 1374, 1483, 3334
Hin1I GRCGYC 1 cut(s) 3547
Hin6I GCGC 4 cut(s) 1212, 1372, 1481, 3332
HinP1I GCGC 4 cut(s) 1212, 1372, 1481, 3332
HincII GTYRAC 1 cut(s) 2694
HindII GTYRAC 1 cut(s) 2694
HinfI GANTC 8 cut(s) 263, 1315, 1412, 1432, 1936, 2212, 2528, 2947
HpaI GTTAAC 1 cut(s) 2694
HpaII CCGG 4 cut(s) 933, 1106, 2126, 3087
HphI GGTGA 9 cut(s) 419, 545, 856, 886, 1287, 1478, 1688, 2754, 2824
Hpy166II GTNNAC 6 cut(s) 635, 1758, 1760, 2694, 2905, 3557
Hpy8I GTNNAC 6 cut(s) 635, 1758, 1760, 2694, 2905, 3557
Hpy99I CGWCG 2 cut(s) 1083, 3564
HpyAV CCTTC 8 cut(s) 281, 349, 1528, 2225, 2252, 2369, 3220, 3382
HpyCH4III ACNGT 9 cut(s) 137, 1128, 1555, 1882, 2108, 2203, 2796, 2801, 3412
HpyCH4IV ACGT 3 cut(s) 1768, 2047, 3562
HpyF10VI GCNNNNNNNGC 8 cut(s) 1390, 1480, 2582, 2587, 2596, 3071, 3236, 3499
HpySE526I ACGT 3 cut(s) 1768, 2047, 3562
Hsp92I GRCGYC 1 cut(s) 3547
HspAI GCGC 4 cut(s) 1212, 1372, 1481, 3332
Ksp22I TGATCA 2 cut(s) 922, 2998
KspAI GTTAAC 1 cut(s) 2694
LguI GCTCTTC 1 cut(s) 953
LmnI GCTCC 3 cut(s) 1486, 2401, 2807
Lsp1109I GCAGC 5 cut(s) 1327, 1402, 1405, 1765, 2176
MaeI CTAG 6 cut(s) 83, 2312, 2364, 2976, 3261, 3440
MaeII ACGT 3 cut(s) 1768, 2047, 3562
MflI RGATCY 4 cut(s) 1426, 1447, 2752, 2925
MhlI GDGCHC 6 cut(s) 699, 965, 1103, 2359, 2812, 3139
MlsI TGGCCA 1 cut(s) 2897
MluNI TGGCCA 1 cut(s) 2897
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 1389
Mox20I TGGCCA 1 cut(s) 2897
MroXI GAANNNNTTC 1 cut(s) 1635
MscI TGGCCA 1 cut(s) 2897
MseI TTAA 9 cut(s) 167, 870, 1088, 1239, 1311, 1331, 1365, 2333, 2693
MslI CAYNNNNRTG 3 cut(s) 561, 1739, 1995
Msp20I TGGCCA 1 cut(s) 2897
MspA1I CMGCKG 1 cut(s) 673
MspCI CTTAAG 1 cut(s) 1330
MspI CCGG 4 cut(s) 933, 1106, 2126, 3087
MvaI CCWGG 8 cut(s) 601, 1703, 2380, 2403, 2484, 2601, 2993, 3448
MwoI GCNNNNNNNGC 8 cut(s) 1390, 1480, 2582, 2587, 2596, 3071, 3236, 3499
NciI CCSGG 2 cut(s) 2127, 3088
NcoI CCATGG 2 cut(s) 3092, 3253
NlaIV GGNNCC 5 cut(s) 1327, 2226, 2754, 3125, 3270
NmeAIII GCCGAG 2 cut(s) 796, 2464
NmuCI GTSAC 6 cut(s) 680, 767, 1018, 1293, 2056, 2286
NsbI TGCGCA 1 cut(s) 1482
NspI RCATGY 1 cut(s) 1522
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 556, 1975
PceI AGGCCT 2 cut(s) 1015, 2974
PciSI GCTCTTC 1 cut(s) 953
PdmI GAANNNNTTC 1 cut(s) 1635
PfeI GAWTC 7 cut(s) 1315, 1412, 1432, 1936, 2212, 2528, 2947
PflMI CCANNNNNTGG 2 cut(s) 1463, 3373
PfoI TCCNGGA 2 cut(s) 2378, 2482
PkrI GCNGC 6 cut(s) 1342, 1345, 1392, 1395, 1780, 2166
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 1769
PpuMI RGGWCCY 2 cut(s) 1326, 3268
PsiI TTATAA 2 cut(s) 717, 2508
Psp124BI GAGCTC 3 cut(s) 965, 1103, 3139
Psp5II RGGWCCY 2 cut(s) 1326, 3268
Psp6I CCWGG 8 cut(s) 599, 1701, 2378, 2401, 2482, 2599, 2991, 3446
PspFI CCCAGC 2 cut(s) 2576, 2585
PspGI CCWGG 8 cut(s) 599, 1701, 2378, 2401, 2482, 2599, 2991, 3446
PspN4I GGNNCC 5 cut(s) 1327, 2226, 2754, 3125, 3270
PspPI GGNCC 7 cut(s) 1326, 1871, 2597, 2905, 3089, 3123, 3268
PspPPI RGGWCCY 2 cut(s) 1326, 3268
PspXI VCTCGAGB 1 cut(s) 32
PstNI CAGNNNCTG 2 cut(s) 1463, 2524
PsuI RGATCY 4 cut(s) 1426, 1447, 2752, 2925
RsaI GTAC 5 cut(s) 38, 1759, 1892, 2593, 2798
RsaNI GTAC 5 cut(s) 37, 1758, 1891, 2592, 2797
RseI CAYNNNNRTG 3 cut(s) 561, 1739, 1995
SacI GAGCTC 3 cut(s) 965, 1103, 3139
SapI GCTCTTC 1 cut(s) 953
SaqAI TTAA 9 cut(s) 167, 870, 1088, 1239, 1311, 1331, 1365, 2333, 2693
SatI GCNGC 6 cut(s) 1341, 1344, 1391, 1394, 1779, 2165
Sau96I GGNCC 7 cut(s) 1326, 1871, 2597, 2905, 3089, 3123, 3268
SchI GAGTC 1 cut(s) 257
SduI GDGCHC 6 cut(s) 699, 965, 1103, 2359, 2812, 3139
SfcI CTRYAG 4 cut(s) 1284, 1788, 2142, 2207
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 4 cut(s) 1326, 2905, 3123, 3268
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 3 cut(s) 561, 1739, 1995
SmlI CTYRAG 4 cut(s) 32, 1226, 1330, 1622
SmoI CTYRAG 4 cut(s) 32, 1226, 1330, 1622
SseBI AGGCCT 2 cut(s) 1015, 2974
SsiI CCGC 9 cut(s) 205, 673, 995, 1343, 2032, 2614, 2638, 2873, 3512
SspI AATATT 1 cut(s) 2434
SspMI CTAG 6 cut(s) 83, 2312, 2364, 2976, 3261, 3440
SstI GAGCTC 3 cut(s) 965, 1103, 3139
StuI AGGCCT 2 cut(s) 1015, 2974
StyI CCWWGG 6 cut(s) 45, 1439, 2908, 2975, 3092, 3253
TaaI ACNGT 9 cut(s) 137, 1128, 1555, 1882, 2108, 2203, 2796, 2801, 3412
TaiI ACGT 3 cut(s) 1771, 2050, 3565
TaqI TCGA 5 cut(s) 33, 266, 2103, 2457, 2814
TaqII GACCGA 3 cut(s) 408, 600, 3111
TatI WGTACW 3 cut(s) 1757, 1890, 2796
TauI GCSGC 1 cut(s) 1346
TfiI GAWTC 7 cut(s) 1315, 1412, 1432, 1936, 2212, 2528, 2947
Tru1I TTAA 9 cut(s) 167, 870, 1088, 1239, 1311, 1331, 1365, 2333, 2693
Tru9I TTAA 9 cut(s) 167, 870, 1088, 1239, 1311, 1331, 1365, 2333, 2693
TseFI GTSAC 6 cut(s) 680, 767, 1018, 1293, 2056, 2286
TseI GCWGC 5 cut(s) 1340, 1390, 1393, 1778, 2164
Tsp45I GTSAC 6 cut(s) 680, 767, 1018, 1293, 2056, 2286
TspGWI ACGGA 1 cut(s) 2237
Van91I CCANNNNNTGG 2 cut(s) 1463, 3373
Vha464I CTTAAG 1 cut(s) 1330
VpaK11BI GGWCC 4 cut(s) 1326, 2905, 3123, 3268
XapI RAATTY 5 cut(s) 152, 1811, 2670, 3014, 3208
XceI RCATGY 1 cut(s) 1522
XcmI CCANNNNNNNNNTGG 2 cut(s) 2713, 3430
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2975
XmiI GTMKAC 1 cut(s) 634
XmnI GAANNNNTTC 1 cut(s) 1635
XspI CTAG 6 cut(s) 83, 2312, 2364, 2976, 3261, 3440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.