MD15G1069000.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000671
Physical Location & Seq
Reverse (-)
4758550 .. 4759968
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1069000.v1.1.491

Sequence Viewer

Length: 1419 bp
ATGGCTGCTGCTGCTGCTGCTGTTGCTGATTGTTGGAAGAATCTGGAATACCTTTCCTTGGTTTCCAAGGTATTTTCGGAACTCGAGACACATCTGTCGTTGGGGGACAAAGTTGTGGCGGAGTTTGTAATTGACATGGGGCTAAAGTACCAGACGGCAGATGAGTTCGATGCCAAATTGAAGGAAAACGGTGTTAATCTGCCCGATTACACCGGCCGTGCACTCCTCACTGCTATTCATACAATTCTTCCTCCGAAACCAAAGGCCGAAAAGGTGTCGAAGAAACAGAGCGGTTCTGATTGTAAGAATGCCACCAAGGTTATGGCTCTAGATCTTAATGATAATAAGAGAGGGGATAGGGATCAGCAGAATGAGCAGCGCTTTTCTGATGAGCCAGAATTGTATAGGGTTTATAAGGGAAGGGTTTCGAGGGTGATGGGCAGCGGCTGCTTCGTTCGGTTGAGTGGTTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCACAGATTGCAAATCGGAGAATTAGTAATGCTAAGAATGTGGTGAGGAGGGATGAGGAGGTTTATGTTAAGGTGATTTCAATTTCGGGTCAGAAGCTGAGCCTGTCGATAAGGGATGTTGATCAGCATACCGGAAAGGATTTAGTTCCCTTGAAGAGGAAAAGATCGGAGGAGGATGACACTCCTAGCACAAATCCACAGGGGCCAAGGGACGGGCATGGAAGTAGGACTGGCCTCTCTGGGATTAGGATTATGGATGAGGATGATGCTGTCCCATCACGCCGGAGGGCATTGAAGAGAATGAGCTTGCCGGAGAGGTGGGAAGCGAAACAGTTGATTGCCGCGGGTGTTCTGAGTGCGACAGAGCATCCAATGTATGATGAGGAAACAGATGGCATGCTTTATCAAGAAGAGGGAGCTGAGGAACAGACGGAGATTGATAACAGTGAGGACCTGCCACAGTTTTTGCTTGGGGAGGGCGGGTATTTTGGTGAGATGTTGCCCGTGAGAATCGTTGAGAATCCAGAAGGGTCATTGAGGCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGGGAAGTGCACGATCAACAGCAGAGAGCATTGATGGATTCTATCCCGAAGGATCTGAATCGTCCCTGGGAAGATCCGATGCCAGAGAATGGCGAGAGACATCTTGCGCAGGAGCTTAGAGGTGTTGGTTTGTCAACAACCTATGAAATGCCCAAGTGGAAGAAAGAGGCCTATGGGAAAACCATTAGTTTCGGGCAGAGATCAAATCTCTCCATTCAGGAACAGAGGCAGAGCTTGCCTATCTACAAGCTGAAGGAAAAACTGATCCAGGCGGTGATTGAAAATCAAGTGCTCATCGTCATTGGTGAGACCGGTTGTGGTAAGACAACACAGGTAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

473

Amino Acids

52.95

Weight (kDa)

6.17

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 136 - 203 4.5e-11 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AasI GACNNNNNNGTC 1 cut(s) 94
Acc16I TGCGCA 1 cut(s) 1185
Acc36I ACCTGC 1 cut(s) 960
AccB7I CCANNNNNTGG 1 cut(s) 1166
AccBSI CCGCTC 1 cut(s) 291
AccII CGCG 1 cut(s) 842
AciI CCGC 7 cut(s) 119, 291, 444, 840, 842, 978, 1349
AclWI GGATC 4 cut(s) 369, 1137, 1145, 1336
AcoI YGGCCR 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 1349
AfaI GTAC 1 cut(s) 149
AfeI AGCGCT 1 cut(s) 380
AfiI CCNNNNNNNGG 4 cut(s) 58, 654, 710, 1166
AgeI ACCGGT 1 cut(s) 1388
AgsI TTSAA 6 cut(s) 181, 579, 652, 793, 1055, 1358
AjnI CCWGG 2 cut(s) 1142, 1344
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 7 cut(s) 595, 804, 917, 1046, 1192, 1311, 1327
AluI AGCT 7 cut(s) 595, 804, 917, 1046, 1192, 1311, 1327
Alw21I GWGCWC 3 cut(s) 223, 1089, 1371
Alw26I GTCTC 3 cut(s) 80, 1168, 1379
Alw44I GTGCAC 2 cut(s) 219, 1085
AlwI GGATC 4 cut(s) 369, 1137, 1145, 1336
AlwNI CAGNNNCTG 2 cut(s) 447, 595
Ama87I CYCGRG 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 380
AoxI GGCC 5 cut(s) 214, 264, 701, 730, 1245
ApaLI GTGCAC 2 cut(s) 219, 1085
AsiGI ACCGGT 1 cut(s) 1388
Asp700I GAANNNNTTC 1 cut(s) 424
AspLEI GCGC 2 cut(s) 381, 1186
AspS9I GGNCC 2 cut(s) 701, 949
AsuHPI GGTGA 6 cut(s) 445, 553, 583, 1001, 1363, 1394
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 1 cut(s) 949
BaeGI GKGCMC 2 cut(s) 223, 1089
Bbv12I GWGCWC 3 cut(s) 223, 1089, 1371
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 6 cut(s) 4, 388, 434, 453, 1033, 1055
BccI CCATC 4 cut(s) 430, 781, 884, 1105
BceAI ACGGC 2 cut(s) 171, 201
BciT130I CCWGG 2 cut(s) 1144, 1346
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 3 cut(s) 80, 1168, 1379
BfaI CTAG 2 cut(s) 329, 684
BfoI RGCGCY 1 cut(s) 382
BfuAI ACCTGC 1 cut(s) 960
BglII AGATCT 1 cut(s) 331
BlpI GCTNAGC 1 cut(s) 596
Bme1390I CCNGG 2 cut(s) 1144, 1346
Bme18I GGWCC 1 cut(s) 949
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 701, 949
BmiI GGNNCC 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 1144, 1346
BmsI GCATC 4 cut(s) 160, 754, 874, 1146
Bpu10I CCTNAGC 1 cut(s) 918
Bpu1102I GCTNAGC 1 cut(s) 596
BsaBI GATNNNNATC 3 cut(s) 360, 618, 1134
BsaI GGTCTC 1 cut(s) 1379
BsaJI CCNNGG 7 cut(s) 57, 66, 315, 704, 840, 1142, 1143
BsaWI WCCGGW 2 cut(s) 629, 1388
BsaXI ACNNNNNCTCC 4 cut(s) 508, 538, 965, 995
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 654, 710, 1166
Bse118I RCCGGY 2 cut(s) 212, 1388
Bse1I ACTGG 1 cut(s) 733
Bse8I GATNNNNATC 3 cut(s) 360, 618, 1134
BseBI CCWGG 2 cut(s) 1144, 1346
BseDI CCNNGG 7 cut(s) 57, 66, 315, 704, 840, 1142, 1143
BseGI GGATG 6 cut(s) 556, 619, 679, 760, 766, 865
BseJI GATNNNNATC 3 cut(s) 360, 618, 1134
BseLI CCNNNNNNNGG 4 cut(s) 58, 654, 710, 1166
BseMII CTCAG 3 cut(s) 587, 842, 909
BseNI ACTGG 1 cut(s) 733
BseRI GAGGAG 4 cut(s) 215, 559, 569, 683
BseSI GKGCMC 2 cut(s) 223, 1089
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 6 cut(s) 4, 388, 434, 453, 1033, 1055
BsgI GTGCAG 3 cut(s) 1032, 1044, 1062
Bsh1236I CGCG 1 cut(s) 842
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 5 cut(s) 216, 266, 703, 732, 1247
BshTI ACCGGT 1 cut(s) 1388
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 3 cut(s) 223, 1089, 1371
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 5 cut(s) 213, 630, 781, 809, 1389
BslFI GGGAC 4 cut(s) 119, 722, 755, 1125
BslI CCNNNNNNNGG 4 cut(s) 58, 654, 710, 1166
BsmAI GTCTC 3 cut(s) 80, 1168, 1379
BsmFI GGGAC 4 cut(s) 119, 722, 755, 1125
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 5 cut(s) 216, 266, 703, 732, 1247
Bso31I GGTCTC 1 cut(s) 1379
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 3 cut(s) 223, 1089, 1371
Bsp143I GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
Bsp1720I GCTNAGC 1 cut(s) 596
BspACI CCGC 7 cut(s) 119, 291, 444, 840, 842, 978, 1349
BspANI GGCC 5 cut(s) 216, 266, 703, 732, 1247
BspCNI CTCAG 3 cut(s) 588, 843, 910
BspFNI CGCG 1 cut(s) 842
BspLI GGNNCC 1 cut(s) 702
BspMI ACCTGC 1 cut(s) 960
BspPI GGATC 4 cut(s) 369, 1137, 1145, 1336
BspTNI GGTCTC 1 cut(s) 1379
BsrBI CCGCTC 1 cut(s) 291
BsrFI RCCGGY 2 cut(s) 212, 1388
BsrI ACTGG 1 cut(s) 733
BssAI RCCGGY 2 cut(s) 212, 1388
BssECI CCNNGG 7 cut(s) 57, 66, 315, 704, 840, 1142, 1143
BssMI GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
BssT1I CCWWGG 4 cut(s) 57, 66, 315, 704
Bst2UI CCWGG 2 cut(s) 1144, 1346
Bst4CI ACNGT 4 cut(s) 191, 831, 944, 960
Bst6I CTCTTC 3 cut(s) 647, 788, 903
BstAPI GCANNNNNTGC 2 cut(s) 447, 1312
BstC8I GCNNGC 4 cut(s) 806, 896, 1041, 1313
BstDEI CTNAG 5 cut(s) 531, 596, 851, 918, 1193
BstDSI CCRYGG 1 cut(s) 840
BstENI CCTNNNNNAGG 1 cut(s) 652
BstF5I GGATG 6 cut(s) 556, 619, 679, 760, 766, 865
BstFNI CGCG 1 cut(s) 842
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 2 cut(s) 381, 1186
BstKTI GATC 9 cut(s) 334, 364, 622, 665, 1093, 1132, 1153, 1280, 1344
BstMAI GTCTC 3 cut(s) 80, 1168, 1379
BstMBI GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 17, 23, 373, 447, 1312
BstNI CCWGG 2 cut(s) 1144, 1346
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 2 cut(s) 1142, 1344
BstSLI GKGCMC 2 cut(s) 223, 1089
BstUI CGCG 1 cut(s) 842
BstV1I GCAGC 6 cut(s) 4, 388, 434, 453, 1033, 1055
BstX2I RGATCY 3 cut(s) 331, 1129, 1150
BstXI CCANNNNNNTGG 1 cut(s) 322
BstYI RGATCY 3 cut(s) 331, 1129, 1150
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 5 cut(s) 216, 266, 703, 732, 1247
BtgI CCRYGG 1 cut(s) 840
BtsCI GGATG 6 cut(s) 556, 619, 679, 760, 766, 865
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 2 cut(s) 228, 949
BveI ACCTGC 1 cut(s) 960
Cac8I GCNNGC 4 cut(s) 806, 896, 1041, 1313
CaiI CAGNNNCTG 2 cut(s) 447, 595
CfoI GCGC 2 cut(s) 381, 1186
Cfr10I RCCGGY 2 cut(s) 212, 1388
Cfr13I GGNCC 2 cut(s) 701, 949
Cfr42I CCGCGG 1 cut(s) 843
Csp6I GTAC 1 cut(s) 148
CspAI ACCGGT 1 cut(s) 1388
CspCI CAANNNNNGTGG 2 cut(s) 945, 980
CviAII CATG 4 cut(s) 136, 494, 716, 895
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 5 cut(s) 531, 596, 851, 918, 1193
DpnI GATC 9 cut(s) 333, 363, 621, 664, 1092, 1131, 1152, 1279, 1343
DpnII GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
DrdI GACNNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 3 cut(s) 647, 788, 903
EarI CTCTTC 3 cut(s) 647, 788, 903
EciI GGCGGA 1 cut(s) 134
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 4 cut(s) 57, 66, 315, 704
Eco147I AGGCCT 1 cut(s) 1247
Eco31I GGTCTC 1 cut(s) 1379
Eco47I GGWCC 1 cut(s) 949
Eco47III AGCGCT 1 cut(s) 380
Eco52I CGGCCG 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 1349
Eco88I CYCGRG 1 cut(s) 83
EcoNI CCTNNNNNAGG 1 cut(s) 652
EcoO109I RGGNCCY 1 cut(s) 949
EcoRII CCWGG 2 cut(s) 1142, 1344
EcoT14I CCWWGG 4 cut(s) 57, 66, 315, 704
ErhI CCWWGG 4 cut(s) 57, 66, 315, 704
FaeI CATG 4 cut(s) 139, 497, 719, 898
FaqI GGGAC 4 cut(s) 119, 722, 755, 1125
FatI CATG 4 cut(s) 135, 493, 715, 894
FauI CCCGC 2 cut(s) 835, 971
FbaI TGATCA 1 cut(s) 619
FokI GGATG 6 cut(s) 563, 626, 686, 767, 773, 852
FspBI CTAG 2 cut(s) 329, 684
FspI TGCGCA 1 cut(s) 1185
GlaI GCGC 2 cut(s) 380, 1185
HaeII RGCGCY 1 cut(s) 382
HaeIII GGCC 5 cut(s) 216, 266, 703, 732, 1247
HapII CCGG 5 cut(s) 213, 630, 781, 809, 1389
HhaI GCGC 2 cut(s) 381, 1186
Hin1II CATG 4 cut(s) 139, 497, 719, 898
Hin6I GCGC 2 cut(s) 379, 1184
HinP1I GCGC 2 cut(s) 379, 1184
HincII GTYRAC 1 cut(s) 1212
HindII GTYRAC 1 cut(s) 1212
HinfI GANTC 5 cut(s) 40, 1008, 1018, 1115, 1135
HpaII CCGG 5 cut(s) 213, 630, 781, 809, 1389
HphI GGTGA 6 cut(s) 445, 553, 583, 1001, 1363, 1394
Hpy166II GTNNAC 3 cut(s) 221, 1087, 1212
Hpy188III TCNNGA 7 cut(s) 44, 85, 329, 905, 1022, 1123, 1295
Hpy8I GTNNAC 3 cut(s) 221, 1087, 1212
HpyAV CCTTC 6 cut(s) 175, 414, 472, 1019, 1120, 1324
HpyCH4III ACNGT 4 cut(s) 191, 831, 944, 960
HpyCH4V TGCA 6 cut(s) 221, 509, 1043, 1049, 1061, 1087
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 17, 23, 373, 447, 1312
HpyF3I CTNAG 5 cut(s) 531, 596, 851, 918, 1193
Hsp92II CATG 4 cut(s) 139, 497, 719, 898
HspAI GCGC 2 cut(s) 379, 1184
Ksp22I TGATCA 1 cut(s) 619
KspI CCGCGG 1 cut(s) 843
Kzo9I GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
LmnI GCTCC 3 cut(s) 914, 1072, 1189
Lsp1109I GCAGC 6 cut(s) 4, 388, 434, 453, 1033, 1055
LweI GCATC 4 cut(s) 160, 754, 874, 1146
MaeI CTAG 2 cut(s) 329, 684
MaeIII GTNAC 1 cut(s) 1411
MalI GATC 9 cut(s) 333, 363, 621, 664, 1092, 1131, 1152, 1279, 1343
MbiI CCGCTC 1 cut(s) 291
MboI GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
MboII GAAGA 8 cut(s) 49, 239, 292, 664, 805, 920, 1160, 1249
MflI RGATCY 3 cut(s) 331, 1129, 1150
MhlI GDGCHC 3 cut(s) 223, 1089, 1371
MluCI AATT 6 cut(s) 129, 176, 243, 398, 519, 579
MmeI TCCRAC 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 4 cut(s) 195, 336, 567, 1068
MspA1I CMGCKG 3 cut(s) 444, 842, 1046
MspI CCGG 5 cut(s) 213, 630, 781, 809, 1389
MspR9I CCNGG 2 cut(s) 1144, 1346
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 2 cut(s) 1144, 1346
MvnI CGCG 1 cut(s) 842
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 17, 23, 373, 447, 1312
NdeII GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
NlaIII CATG 4 cut(s) 139, 497, 719, 898
NlaIV GGNNCC 1 cut(s) 702
NsbI TGCGCA 1 cut(s) 1185
NspI RCATGY 1 cut(s) 898
PaeI GCATGC 1 cut(s) 898
PaeR7I CTCGAG 1 cut(s) 83
PasI CCCWGGG 1 cut(s) 1143
PceI AGGCCT 1 cut(s) 1247
PctI GAATGC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 5 cut(s) 40, 1008, 1018, 1115, 1135
PflMI CCANNNNNTGG 1 cut(s) 1166
PinAI ACCGGT 1 cut(s) 1388
PpuMI RGGWCCY 1 cut(s) 949
PsiI TTATAA 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 949
Psp6I CCWGG 2 cut(s) 1142, 1344
PspGI CCWGG 2 cut(s) 1142, 1344
PspN4I GGNNCC 1 cut(s) 702
PspPI GGNCC 2 cut(s) 701, 949
PspPPI RGGWCCY 1 cut(s) 949
PstNI CAGNNNCTG 2 cut(s) 447, 595
PsuI RGATCY 3 cut(s) 331, 1129, 1150
PvuII CAGCTG 1 cut(s) 1046
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacII CCGCGG 1 cut(s) 843
SaqAI TTAA 4 cut(s) 195, 336, 567, 1068
Sau3AI GATC 9 cut(s) 331, 361, 619, 662, 1090, 1129, 1150, 1277, 1341
Sau96I GGNCC 2 cut(s) 701, 949
ScrFI CCNGG 2 cut(s) 1144, 1346
SduI GDGCHC 3 cut(s) 223, 1089, 1371
SfaNI GCATC 4 cut(s) 160, 754, 874, 1146
Sfr274I CTCGAG 1 cut(s) 83
Sfr303I CCGCGG 1 cut(s) 843
SgrBI CCGCGG 1 cut(s) 843
SinI GGWCC 1 cut(s) 949
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
SphI GCATGC 1 cut(s) 898
Sse9I AATT 6 cut(s) 129, 176, 243, 398, 519, 579
SseBI AGGCCT 1 cut(s) 1247
SsiI CCGC 7 cut(s) 119, 291, 444, 840, 842, 978, 1349
SspMI CTAG 2 cut(s) 329, 684
StuI AGGCCT 1 cut(s) 1247
StyD4I CCNGG 2 cut(s) 1142, 1344
StyI CCWWGG 4 cut(s) 57, 66, 315, 704
TaaI ACNGT 4 cut(s) 191, 831, 944, 960
TaqI TCGA 5 cut(s) 84, 168, 278, 428, 605
TasI AATT 6 cut(s) 129, 176, 243, 398, 519, 579
TauI GCSGC 2 cut(s) 447, 842
TfiI GAWTC 5 cut(s) 40, 1008, 1018, 1115, 1135
Tru1I TTAA 4 cut(s) 195, 336, 567, 1068
Tru9I TTAA 4 cut(s) 195, 336, 567, 1068
TscAI CASTG 2 cut(s) 235, 949
TspDTI ATGAA 3 cut(s) 227, 482, 1236
TspGWI ACGGA 1 cut(s) 944
TspRI CASTG 2 cut(s) 235, 949
Van91I CCANNNNNTGG 1 cut(s) 1166
VneI GTGCAC 2 cut(s) 219, 1085
VpaK11BI GGWCC 1 cut(s) 949
XagI CCTNNNNNAGG 1 cut(s) 652
XbaI TCTAGA 1 cut(s) 328
XceI RCATGY 1 cut(s) 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 319
XhoI CTCGAG 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 2 cut(s) 329, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.