Prupe.2G077900_v2.0.a1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000671
Physical Location & Seq
Reverse (-)
12002071 .. 12007571
5501 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G077900.2

Sequence Viewer

Length: 3588 bp
ATGGCTGCCACTGACGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTCGAAACCCATATAGGGGTTGGGGACAAAGTTCTGGCGGAGTTCATCACTGAGTTGGGACGAAAGTGCGAGACGGTGGACGAGTTTGACACCAAATTAAAGAAAAACGGTGCCGAGATGCCCGATTACTTTGTCCGTACACTCCTCACTATCATTCACGCTATTCTTCCACCAAAGCCAAAGCCTGAGAAGGACTCGAAGAAAGAGAGCGCTTCTGATGGTAGGAAGACCAAGTTTAAGGCTTTGGCAGTCGCTGATAATAAAGATAGAGTCAAGGACATCGAGAAGGAAATTGAGATGGAGACCAAGGAAAAGCGAAACAGAAGAGAAGAACAAGATGAAGAGCGAGAAGAAGATAGGCGTAGAGGTAGTGATAGATATAGGGAAGGAGATAGAGATAGAGATCGAGATAGACACAGAGGAGGTAGAGATAGAGATGGGAGGGAGAGGCGAAGGGAAAGGTATGATAGAGATGAGAGGCGCAGAGATACACATTATAATGATGATGATGATGACAGGGGAAGGCATACAGATCGATACAATAAGCATAAGAGAGATAGGTATGAAGACGATGGAGATGTTAAGGAAGATGGTGATGATAGGAGAGGTAATAGGGATCGGCAGAACGGCCAGAACCATTCGGATGAGCCCGAATTGTATCAGGTTTATAAGGGCAGGGTTTCGAGAGTGATGGACACCGGTTGTTTTGTTCAGTTGAATGATTTAAGAGGGAAGGAGGGTCTGGTTCACGTTTCACAGATGGCAACTAGGCGAATTAGCAATGCTAAGGATGTGGTGAAGAGGGATCAGGAAGTTTATGTGAAGGTGATTTCAATTTCAGGTCAGAAGTTGAGCCTTTCAATGAGGGATGTTGATCAACATACAGGGAAGGATTTGCTCCCCTTGAAGAAGAGCTCAGAGGATGATGCTCTTAGGACGAACCCATCTTTTTCAAAGGATGGACCTGTGACTAGGACTGGTCTTTCTGGGATTAGGATTGTGGAAGAGGATGATGTTGGCCCATCACGTCGGCCATTGAAGAGAATGAGCTCGCCAGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAGTATCCTATGTATGATGAGGAAACCGATGGGATGCTTTATCAAGAAGAGGGAGCTGAGGAAGAGCTTGAGATTGAGCTCAATGAGGACGAGCCAGCCTTTTTAAATGGACAGAGCAGGTATTCTGTTGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAAGGGTCACTGGGTCGTGCAGCTGCACTTCAGTCCGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAGAGAACCATGTTGGATTCTATCCCAAAGGATCTCAATCGTCCCTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAAGAACTTAGAGGTGTTGGTTTGTCAGCATATGATATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAAACTATCAGTTTTGGGCAGAGGTCAAAACTCTCAATCCAGGAACAGAGGCAGAGCTTGCCTATCTACAAGCTGAAGAAAGAATTGATTGCAGCGGTGCATGAGAACCAAGTGCTTGTCGTCATTGGTGAGACTGGTTCGGGTAAGACAACACAGGTAACGCAGTATCTTGCAGAAGCGGGTTACACGACAATGGGTAAGATTGGTTGTACACAGCCACGTAGGGTGGCTGCTATGTCTGTGGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTTTAGGAGAGGAAGTCGGCTATGCAATTCGTTTTGAGGATTGCACTGGTCCAGACACTGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATTGATGAGAACCTTTCTCAGTATTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCACACAGATGTTCTTTTTGGACTACTGAAGAAGCTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCGGAGAAGTTTTCCGGTTATTTCTTTAACTGTAACATCTTCACTATCCCTGGGAGAACTTTTCCTGTAGAGATACTCTACACTAAACAGCCGGAAAGCGACTACCTTGATGCATCTCTAATAACTGTTCTACAAATACACTTGACAGAACCTGAAGGTGACATCCTTCTTTTCTTGACTGGACAAGAAGAGATTGATTTTGCATGCCAGTCTCTTTATGAGAGGATGAAAGGTCTTGGTAAAAATGTCCCTGAGTTGATTATCCTACCAGTGTATAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATATTTGATCCTGCCCCACCAGGTAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCATCACTGACCATTGATGGGATATTTTATGTCATTGATCCTGGTTTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTGGTCATAACTCCAATTTCCCAAGCATCAGCTAAGCAACGAGCTGGGCGTGCTGGGCGTACAGGCCCTGGGAAATGTTACCGCCTCTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAATTCCAGAAATCCAGAGGATAAATCTTGGGACTACTACACTTACGATGAAAGCTATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCCATGGAACAATTGTACAGTTTAGGAGCACTGGATGAGGAGGGGCTGCTCACCAAATTGGGTAGGAAAATGGCTGAGTTTCCTCTGGATCCACCCTTGTCTAAGATGCTACTGGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATCATTGCAATGATTCAGACTGGTAATATTTTTTACAGGCCTAGGGAAAAACAAGCCCAGGCAGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTGACTTTACTTGCAGTCTATGAGGCATGGAAAGCTAAGAATTTTTCAGGGCCATGGTGTTTTGAGAATTTTGTTCAGTCTCGATCCTTGAGGAGGGCACAGGATGTCAGAAAACAGCTTCTCAGCATCATGGACAAGTATAAACTAGATGTTGTCAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACAGCAGGGTTTTTCTTCCACGGTGCTAGAAAGGACCCGCAGGAGGGTTATAGAACCCTAGTGGAGAACCAACCAGTTTATATACACCCAAGCAGCGCCCTCTTCCAGAGACAACCAGATTGGGTGATCTACCATGAGCTGGTTATGACCACAAAGGAGTACATGCGCGAGGTGACGGTTGTCGACCCCAAATGGCTTGTGGAACTGGCACCCAGGTTCTTCAAAGTGGCAGATCCCACCAAGATGAGCAAGCGCAAGCGTCAAGAACGTATTGAACCGCTATATGATAGATACCATGAACCTAACTCTTGGCGTCTTAGTAAGCGACGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1196

Amino Acids

136.69

Weight (kDa)

6.63

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 567, 738, 2526
Acc36I ACCTGC 1 cut(s) 1284
AccB1I GGYRCC 2 cut(s) 179, 3460
AccB7I CCANNNNNTGG 2 cut(s) 1481, 3391
AccI GTMKAC 1 cut(s) 3435
AccII CGCG 1 cut(s) 3420
AcoI YGGCCR 4 cut(s) 697, 1100, 1809, 2913
AcsI RAATTY 3 cut(s) 3097, 3124, 3226
AcuI CTGAAG 5 cut(s) 1352, 1661, 1845, 2054, 2268
AcyI GRCGYC 1 cut(s) 3565
AfaI GTAC 7 cut(s) 38, 208, 1777, 1910, 2611, 2816, 3413
AfeI AGCGCT 1 cut(s) 280
AgeI ACCGGT 1 cut(s) 767
AjiI CACGTC 2 cut(s) 1097, 3581
AjnI CCWGG 8 cut(s) 1457, 1605, 2143, 2419, 2500, 2617, 3009, 3464
Alw21I GWGCWC 5 cut(s) 72, 986, 1121, 1257, 2830
Alw26I GTCTC 8 cut(s) 134, 365, 1472, 1691, 2047, 2310, 3141, 3355
AlwNI CAGNNNCTG 5 cut(s) 323, 1481, 1898, 2246, 2618
Ama87I CYCGRG 1 cut(s) 32
Aor51HI AGCGCT 1 cut(s) 280
AoxI GGCC 8 cut(s) 697, 1087, 1100, 1809, 2614, 2913, 2990, 3107
ApeKI GCWGC 9 cut(s) 5, 1358, 1361, 1408, 1411, 1658, 1796, 2845, 3345
ApoI RAATTY 3 cut(s) 3097, 3124, 3226
ArsI GACNNNNNNTTYG 2 cut(s) 2046, 2078
AsiGI ACCGGT 1 cut(s) 767
AspA2I CCTAGG 1 cut(s) 2993
AspLEI GCGC 5 cut(s) 281, 552, 3350, 3420, 3507
AspS9I GGNCC 7 cut(s) 1031, 1088, 1889, 2615, 2923, 3107, 3286
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 4 cut(s) 1031, 1889, 2923, 3286
AvrII CCTAGG 1 cut(s) 2993
AxyI CCTNAGG 1 cut(s) 1156
BaeGI GKGCMC 2 cut(s) 2377, 3157
BalI TGGCCA 2 cut(s) 1811, 2915
BamHI GGATCC 2 cut(s) 2770, 2887
BanI GGYRCC 2 cut(s) 179, 3460
BanII GRGCYC 5 cut(s) 72, 720, 986, 1121, 1257
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 302, 642
Bbv12I GWGCWC 5 cut(s) 72, 986, 1121, 1257, 2830
BbvCI CCTCAGC 2 cut(s) 1233, 2457
BbvI GCAGC 8 cut(s) 1348, 1370, 1420, 1423, 1670, 1783, 2832, 3357
BceAI ACGGC 1 cut(s) 712
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
BciVI GTATCC 1 cut(s) 1191
BclI TGATCA 1 cut(s) 943
BcoDI GTCTC 8 cut(s) 134, 365, 1472, 1691, 2047, 2310, 3141, 3355
BfaI CTAG 8 cut(s) 837, 1041, 2382, 2543, 2994, 3203, 3279, 3311
BfmI CTRYAG 1 cut(s) 2160
BfoI RGCGCY 2 cut(s) 282, 3351
BfuAI ACCTGC 1 cut(s) 1284
BfuI GTATCC 1 cut(s) 1191
BglII AGATCT 1 cut(s) 2943
BisI GCNGC 9 cut(s) 6, 1359, 1362, 1409, 1412, 1659, 1797, 2846, 3346
BlnI CCTAGG 1 cut(s) 2993
BlpI GCTNAGC 1 cut(s) 2583
BlsI GCNGC 9 cut(s) 7, 1360, 1363, 1410, 1413, 1660, 1798, 2847, 3347
Bme1390I CCNGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
Bme18I GGWCC 4 cut(s) 1031, 1889, 2923, 3286
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 2 cut(s) 1097, 3581
BmgT120I GGNCC 7 cut(s) 1031, 1088, 1889, 2615, 2923, 3107, 3286
BmiI GGNNCC 5 cut(s) 181, 2772, 2889, 3288, 3462
BmrFI CCNGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
BmrI ACTGGG 1 cut(s) 1358
BmuI ACTGGG 1 cut(s) 1358
BoxI GACNNNNGTC 1 cut(s) 3431
BpiI GAAGAC 2 cut(s) 302, 642
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 3 cut(s) 855, 1233, 2457
Bpu1102I GCTNAGC 1 cut(s) 2583
BpuEI CTTGAG 2 cut(s) 1265, 3166
Bsa29I ATCGAT 1 cut(s) 604
BsaAI YACGTR 1 cut(s) 1787
BsaBI GATNNNNATC 3 cut(s) 471, 942, 1449
BsaHI GRCGYC 1 cut(s) 3565
BsaI GGTCTC 2 cut(s) 365, 2047
BsaWI WCCGGW 2 cut(s) 767, 2108
BsaXI ACNNNNNCTCC 2 cut(s) 1839, 1869
Bse118I RCCGGY 1 cut(s) 767
Bse21I CCTNAGG 1 cut(s) 1156
Bse3DI GCAATG 4 cut(s) 856, 2665, 2955, 2967
Bse8I GATNNNNATC 3 cut(s) 471, 942, 1449
BseBI CCWGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
BseCI ATCGAT 1 cut(s) 604
BseJI GATNNNNATC 3 cut(s) 471, 942, 1449
BseMI GCAATG 4 cut(s) 856, 2665, 2955, 2967
BseRI GAGGAG 4 cut(s) 203, 504, 2852, 3163
BseSI GKGCMC 2 cut(s) 2377, 3157
BseXI GCAGC 8 cut(s) 1348, 1370, 1420, 1423, 1670, 1783, 2832, 3357
BseYI CCCAGC 2 cut(s) 2594, 2603
BsgI GTGCAG 2 cut(s) 1347, 1377
Bsh1236I CGCG 1 cut(s) 3420
BshFI GGCC 8 cut(s) 699, 1089, 1102, 1811, 2616, 2915, 2992, 3109
BshNI GGYRCC 2 cut(s) 179, 3460
BshTI ACCGGT 1 cut(s) 767
BshVI ATCGAT 1 cut(s) 604
BsiHKAI GWGCWC 5 cut(s) 72, 986, 1121, 1257, 2830
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 3 cut(s) 768, 2109, 2186
BslFI GGGAC 6 cut(s) 107, 141, 1440, 2327, 2653, 2722
BsmAI GTCTC 8 cut(s) 134, 365, 1472, 1691, 2047, 2310, 3141, 3355
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 6 cut(s) 107, 141, 1440, 2327, 2653, 2722
BsnI GGCC 8 cut(s) 699, 1089, 1102, 1811, 2616, 2915, 2992, 3109
Bso31I GGTCTC 2 cut(s) 365, 2047
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 8 cut(s) 72, 720, 986, 1121, 1257, 2377, 2830, 3157
Bsp1407I TGTACA 2 cut(s) 1775, 2814
Bsp1720I GCTNAGC 1 cut(s) 2583
Bsp19I CCATGG 2 cut(s) 2802, 3110
BspANI GGCC 8 cut(s) 699, 1089, 1102, 1811, 2616, 2915, 2992, 3109
BspDI ATCGAT 1 cut(s) 604
BspFNI CGCG 1 cut(s) 3420
BspHI TCATGA 1 cut(s) 1993
BspLI GGNNCC 5 cut(s) 181, 2772, 2889, 3288, 3462
BspMI ACCTGC 1 cut(s) 1284
BspQI GCTCTTC 3 cut(s) 405, 974, 1233
BspT107I GGYRCC 2 cut(s) 179, 3460
BspTNI GGTCTC 2 cut(s) 365, 2047
BsrDI GCAATG 4 cut(s) 856, 2665, 2955, 2967
BsrFI RCCGGY 1 cut(s) 767
BsrGI TGTACA 2 cut(s) 1775, 2814
BssAI RCCGGY 1 cut(s) 767
BssNI GRCGYC 1 cut(s) 3565
BssT1I CCWWGG 8 cut(s) 45, 54, 375, 2088, 2802, 2926, 2993, 3110
Bst2UI CCWGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
Bst4CI ACNGT 9 cut(s) 145, 179, 1146, 1900, 2126, 2221, 2819, 3275, 3430
BstACI GRCGYC 1 cut(s) 3565
BstAPI GCANNNNNTGC 1 cut(s) 1624
BstAUI TGTACA 2 cut(s) 1775, 2814
BstBAI YACGTR 1 cut(s) 1787
BstC8I GCNNGC 7 cut(s) 1121, 1272, 1625, 2299, 2601, 3503, 3509
BstDSI CCRYGG 3 cut(s) 2802, 3110, 3271
BstENI CCTNNNNNAGG 1 cut(s) 3148
BstFNI CGCG 1 cut(s) 3420
BstH2I RGCGCY 2 cut(s) 282, 3351
BstHHI GCGC 5 cut(s) 281, 552, 3350, 3420, 3507
BstMAI GTCTC 8 cut(s) 134, 365, 1472, 1691, 2047, 2310, 3141, 3355
BstMWI GCNNNNNNNGC 7 cut(s) 1408, 1624, 2047, 2600, 2605, 3089, 3254
BstNI CCWGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
BstNSI RCATGY 2 cut(s) 2301, 3418
BstPAI GACNNNNGTC 1 cut(s) 3431
BstSCI CCNGG 8 cut(s) 1457, 1605, 2143, 2419, 2500, 2617, 3009, 3464
BstSFI CTRYAG 1 cut(s) 2160
BstSLI GKGCMC 2 cut(s) 2377, 3157
BstUI CGCG 1 cut(s) 3420
BstV1I GCAGC 8 cut(s) 1348, 1370, 1420, 1423, 1670, 1783, 2832, 3357
BstV2I GAAGAC 2 cut(s) 302, 642
BstX2I RGATCY 6 cut(s) 1444, 1465, 2770, 2887, 2943, 3484
BstXI CCANNNNNNTGG 1 cut(s) 1131
BstYI RGATCY 6 cut(s) 1444, 1465, 2770, 2887, 2943, 3484
Bsu15I ATCGAT 1 cut(s) 604
Bsu36I CCTNAGG 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 1191
BsuRI GGCC 8 cut(s) 699, 1089, 1102, 1811, 2616, 2915, 2992, 3109
BsuTUI ATCGAT 1 cut(s) 604
BtgI CCRYGG 3 cut(s) 2802, 3110, 3271
BtrI CACGTC 2 cut(s) 1097, 3581
BtsI GCAGTG 1 cut(s) 2941
BveI ACCTGC 1 cut(s) 1284
Cac8I GCNNGC 7 cut(s) 1121, 1272, 1625, 2299, 2601, 3503, 3509
CaiI CAGNNNCTG 5 cut(s) 323, 1481, 1898, 2246, 2618
CciI TCATGA 1 cut(s) 1993
CfoI GCGC 5 cut(s) 281, 552, 3350, 3420, 3507
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 7 cut(s) 1031, 1088, 1889, 2615, 2923, 3107, 3286
ClaI ATCGAT 1 cut(s) 604
CseI GACGC 2 cut(s) 3500, 3554
CsiI ACCWGGT 1 cut(s) 2419
Csp6I GTAC 7 cut(s) 37, 207, 1776, 1909, 2610, 2815, 3412
CspAI ACCGGT 1 cut(s) 767
CviQI GTAC 7 cut(s) 37, 207, 1776, 1909, 2610, 2815, 3412
DraI TTTAAA 1 cut(s) 1281
EaeI YGGCCR 4 cut(s) 697, 1100, 1809, 2913
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 4 cut(s) 70, 984, 1119, 1255
Eco130I CCWWGG 8 cut(s) 45, 54, 375, 2088, 2802, 2926, 2993, 3110
Eco147I AGGCCT 1 cut(s) 2992
Eco24I GRGCYC 5 cut(s) 72, 720, 986, 1121, 1257
Eco31I GGTCTC 2 cut(s) 365, 2047
Eco47I GGWCC 4 cut(s) 1031, 1889, 2923, 3286
Eco47III AGCGCT 1 cut(s) 280
Eco53kI GAGCTC 4 cut(s) 70, 984, 1119, 1255
Eco57I CTGAAG 5 cut(s) 1352, 1661, 1845, 2054, 2268
Eco81I CCTNAGG 1 cut(s) 1156
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 4 cut(s) 70, 984, 1119, 1255
EcoNI CCTNNNNNAGG 1 cut(s) 3148
EcoO109I RGGNCCY 2 cut(s) 2615, 3286
EcoRII CCWGG 8 cut(s) 1457, 1605, 2143, 2419, 2500, 2617, 3009, 3464
EcoT14I CCWWGG 8 cut(s) 45, 54, 375, 2088, 2802, 2926, 2993, 3110
EcoT22I ATGCAT 1 cut(s) 2209
EcoT38I GRGCYC 5 cut(s) 72, 720, 986, 1121, 1257
ErhI CCWWGG 8 cut(s) 45, 54, 375, 2088, 2802, 2926, 2993, 3110
Esp3I CGTCTC 1 cut(s) 134
FaqI GGGAC 6 cut(s) 107, 141, 1440, 2327, 2653, 2722
FauI CCCGC 2 cut(s) 1738, 3297
FauNDI CATATG 1 cut(s) 1531
FbaI TGATCA 1 cut(s) 943
FblI GTMKAC 1 cut(s) 3435
Fnu4HI GCNGC 9 cut(s) 6, 1359, 1362, 1409, 1412, 1659, 1797, 2846, 3346
FriOI GRGCYC 5 cut(s) 72, 720, 986, 1121, 1257
Fsp4HI GCNGC 9 cut(s) 6, 1359, 1362, 1409, 1412, 1659, 1797, 2846, 3346
FspBI CTAG 8 cut(s) 837, 1041, 2382, 2543, 2994, 3203, 3279, 3311
GlaI GCGC 5 cut(s) 280, 551, 3349, 3419, 3506
GluI GCNGC 9 cut(s) 6, 1359, 1362, 1409, 1412, 1659, 1797, 2846, 3346
GsaI CCCAGC 2 cut(s) 2598, 2607
HaeII RGCGCY 2 cut(s) 282, 3351
HaeIII GGCC 8 cut(s) 699, 1089, 1102, 1811, 2616, 2915, 2992, 3109
HapII CCGG 3 cut(s) 768, 2109, 2186
HgaI GACGC 2 cut(s) 3500, 3554
HhaI GCGC 5 cut(s) 281, 552, 3350, 3420, 3507
Hin1I GRCGYC 1 cut(s) 3565
Hin6I GCGC 5 cut(s) 279, 550, 3348, 3418, 3505
HinP1I GCGC 5 cut(s) 279, 550, 3348, 3418, 3505
HincII GTYRAC 1 cut(s) 3436
HindII GTYRAC 1 cut(s) 3436
HindIII AAGCTT 1 cut(s) 2039
HinfI GANTC 6 cut(s) 263, 339, 1333, 1430, 2546, 2965
HpaII CCGG 3 cut(s) 768, 2109, 2186
Hpy166II GTNNAC 6 cut(s) 148, 209, 817, 1778, 2923, 3436
Hpy188I TCNGA 9 cut(s) 67, 286, 712, 915, 988, 2970, 3021, 3060, 3167
Hpy8I GTNNAC 6 cut(s) 148, 209, 817, 1778, 2923, 3436
Hpy99I CGWCG 2 cut(s) 1101, 3582
HpyCH4III ACNGT 9 cut(s) 145, 179, 1146, 1900, 2126, 2221, 2819, 3275, 3430
HpyCH4IV ACGT 5 cut(s) 819, 1096, 1786, 3520, 3580
HpyF10VI GCNNNNNNNGC 7 cut(s) 1408, 1624, 2047, 2600, 2605, 3089, 3254
HpySE526I ACGT 5 cut(s) 819, 1096, 1786, 3520, 3580
Hsp92I GRCGYC 1 cut(s) 3565
HspAI GCGC 5 cut(s) 279, 550, 3348, 3418, 3505
Ksp22I TGATCA 1 cut(s) 943
LguI GCTCTTC 3 cut(s) 405, 974, 1233
LmnI GCTCC 4 cut(s) 972, 1229, 1387, 2825
Lsp1109I GCAGC 8 cut(s) 1348, 1370, 1420, 1423, 1670, 1783, 2832, 3357
MabI ACCWGGT 1 cut(s) 2419
MaeI CTAG 8 cut(s) 837, 1041, 2382, 2543, 2994, 3203, 3279, 3311
MaeII ACGT 5 cut(s) 819, 1096, 1786, 3520, 3580
MfeI CAATTG 1 cut(s) 2810
MflI RGATCY 6 cut(s) 1444, 1465, 2770, 2887, 2943, 3484
MhlI GDGCHC 8 cut(s) 72, 720, 986, 1121, 1257, 2377, 2830, 3157
MlsI TGGCCA 2 cut(s) 1811, 2915
MluNI TGGCCA 2 cut(s) 1811, 2915
MlyI GAGTC 2 cut(s) 257, 348
MmeI TCCRAC 1 cut(s) 1407
Mox20I TGGCCA 2 cut(s) 1811, 2915
Mph1103I ATGCAT 1 cut(s) 2209
MscI TGGCCA 2 cut(s) 1811, 2915
MseI TTAA 8 cut(s) 167, 306, 651, 794, 1280, 1329, 1383, 2121
MslI CAYNNNNRTG 6 cut(s) 567, 711, 1757, 2013, 2960, 3494
Msp20I TGGCCA 2 cut(s) 1811, 2915
MspA1I CMGCKG 2 cut(s) 1361, 1661
MspI CCGG 3 cut(s) 768, 2109, 2186
MspR9I CCNGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
MunI CAATTG 1 cut(s) 2810
MvaI CCWGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
MvnI CGCG 1 cut(s) 3420
MwoI GCNNNNNNNGC 7 cut(s) 1408, 1624, 2047, 2600, 2605, 3089, 3254
NcoI CCATGG 2 cut(s) 2802, 3110
NdeI CATATG 1 cut(s) 1531
NlaIV GGNNCC 5 cut(s) 181, 2772, 2889, 3288, 3462
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 6 cut(s) 1036, 1311, 1344, 2074, 2252, 3424
NsiI ATGCAT 1 cut(s) 2209
NspI RCATGY 2 cut(s) 2301, 3418
PaeI GCATGC 1 cut(s) 2301
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 1 cut(s) 1993
PasI CCCWGGG 3 cut(s) 1458, 2144, 2618
PceI AGGCCT 1 cut(s) 2992
PciSI GCTCTTC 3 cut(s) 405, 974, 1233
PcsI WCGNNNNNNNCGW 1 cut(s) 2596
PfeI GAWTC 4 cut(s) 1333, 1430, 2546, 2965
PflFI GACNNNGTC 1 cut(s) 1898
PflMI CCANNNNNTGG 2 cut(s) 1481, 3391
PfoI TCCNGGA 1 cut(s) 1605
PinAI ACCGGT 1 cut(s) 767
PkrI GCNGC 9 cut(s) 7, 1360, 1363, 1410, 1413, 1660, 1798, 2847, 3347
PleI GAGTC 2 cut(s) 257, 347
PpsI GAGTC 2 cut(s) 257, 347
Ppu21I YACGTR 1 cut(s) 1787
PpuMI RGGWCCY 1 cut(s) 3286
PshAI GACNNNNGTC 1 cut(s) 3431
PsiI TTATAA 3 cut(s) 567, 738, 2526
Psp124BI GAGCTC 4 cut(s) 72, 986, 1121, 1257
Psp5II RGGWCCY 1 cut(s) 3286
Psp6I CCWGG 8 cut(s) 1457, 1605, 2143, 2419, 2500, 2617, 3009, 3464
PspFI CCCAGC 2 cut(s) 2594, 2603
PspGI CCWGG 8 cut(s) 1457, 1605, 2143, 2419, 2500, 2617, 3009, 3464
PspN4I GGNNCC 5 cut(s) 181, 2772, 2889, 3288, 3462
PspPI GGNCC 7 cut(s) 1031, 1088, 1889, 2615, 2923, 3107, 3286
PspPPI RGGWCCY 1 cut(s) 3286
PspXI VCTCGAGB 1 cut(s) 32
PstNI CAGNNNCTG 5 cut(s) 323, 1481, 1898, 2246, 2618
PsuI RGATCY 6 cut(s) 1444, 1465, 2770, 2887, 2943, 3484
PsyI GACNNNGTC 1 cut(s) 1898
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 7 cut(s) 38, 208, 1777, 1910, 2611, 2816, 3413
RsaNI GTAC 7 cut(s) 37, 207, 1776, 1909, 2610, 2815, 3412
RseI CAYNNNNRTG 6 cut(s) 567, 711, 1757, 2013, 2960, 3494
SacI GAGCTC 4 cut(s) 72, 986, 1121, 1257
SalI GTCGAC 1 cut(s) 3434
SapI GCTCTTC 3 cut(s) 405, 974, 1233
SaqAI TTAA 8 cut(s) 167, 306, 651, 794, 1280, 1329, 1383, 2121
SatI GCNGC 9 cut(s) 6, 1359, 1362, 1409, 1412, 1659, 1797, 2846, 3346
Sau96I GGNCC 7 cut(s) 1031, 1088, 1889, 2615, 2923, 3107, 3286
SchI GAGTC 2 cut(s) 257, 348
ScrFI CCNGG 8 cut(s) 1459, 1607, 2145, 2421, 2502, 2619, 3011, 3466
SduI GDGCHC 8 cut(s) 72, 720, 986, 1121, 1257, 2377, 2830, 3157
SexAI ACCWGGT 1 cut(s) 2419
SfcI CTRYAG 1 cut(s) 2160
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 4 cut(s) 1031, 1889, 2923, 3286
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 6 cut(s) 567, 711, 1757, 2013, 2960, 3494
SmlI CTYRAG 3 cut(s) 32, 1244, 3145
SmoI CTYRAG 3 cut(s) 32, 1244, 3145
SphI GCATGC 1 cut(s) 2301
SseBI AGGCCT 1 cut(s) 2992
SspI AATATT 2 cut(s) 2452, 2980
SspMI CTAG 8 cut(s) 837, 1041, 2382, 2543, 2994, 3203, 3279, 3311
SstI GAGCTC 4 cut(s) 72, 986, 1121, 1257
StuI AGGCCT 1 cut(s) 2992
StyD4I CCNGG 8 cut(s) 1457, 1605, 2143, 2419, 2500, 2617, 3009, 3464
StyI CCWWGG 8 cut(s) 45, 54, 375, 2088, 2802, 2926, 2993, 3110
TaaI ACNGT 9 cut(s) 145, 179, 1146, 1900, 2126, 2221, 2819, 3275, 3430
TaiI ACGT 5 cut(s) 822, 1099, 1789, 3523, 3583
TaqI TCGA 9 cut(s) 33, 72, 266, 351, 475, 604, 752, 3139, 3435
TatI WGTACW 4 cut(s) 1775, 1908, 2814, 3411
TfiI GAWTC 4 cut(s) 1333, 1430, 2546, 2965
Tru1I TTAA 8 cut(s) 167, 306, 651, 794, 1280, 1329, 1383, 2121
Tru9I TTAA 8 cut(s) 167, 306, 651, 794, 1280, 1329, 1383, 2121
TseFI GTSAC 6 cut(s) 1036, 1311, 1344, 2074, 2252, 3424
TseI GCWGC 9 cut(s) 5, 1358, 1361, 1408, 1411, 1658, 1796, 2845, 3345
Tsp45I GTSAC 6 cut(s) 1036, 1311, 1344, 2074, 2252, 3424
TspDTI ATGAA 7 cut(s) 103, 423, 648, 2001, 2336, 2741, 3564
TspGWI ACGGA 1 cut(s) 194
Tth111I GACNNNGTC 1 cut(s) 1898
Van91I CCANNNNNTGG 2 cut(s) 1481, 3391
VpaK11BI GGWCC 4 cut(s) 1031, 1889, 2923, 3286
XagI CCTNNNNNAGG 1 cut(s) 3148
XapI RAATTY 3 cut(s) 3097, 3124, 3226
XceI RCATGY 2 cut(s) 2301, 3418
XcmI CCANNNNNNNNNTGG 2 cut(s) 86, 3448
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2993
XmiI GTMKAC 1 cut(s) 3435
XspI CTAG 8 cut(s) 837, 1041, 2382, 2543, 2994, 3203, 3279, 3311
Zsp2I ATGCAT 1 cut(s) 2209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.