Rh1AG235400

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000671
Physical Location & Seq
Forward (+)
44590103 .. 44594365
4263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG235400.1

Sequence Viewer

Length: 3594 bp
ATGGCTGCGATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCCGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTCGATGCCAAATTGAAGGAACACGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCACGCAATTCTTCCTCCCAAGGCAAAGTCCGAGAACGACTCAAAGAAAGAGAGCACTGCTGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGTTGGAGGCTAAAGAAAAGCGAAATGACAGAGAAGAATGGGGACATGGGCGTGAAGAAGATAGGCCTAGAGGTAGAGATGGAGGTAGAGGTAGGGGTAGGGAAAGAGATACAAATCGGGACAGGGACAGGGACAGGGACAGGGATAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAATCATTATCATGATTATGATAAGGGAAGGCATACAGATCGATCCAATAAGCACGGGAGGGATGAGTATGAAAAGGATGGAGATGTTAGAGAAGATGATATTGATAGGAGAGGGAGTAGGGATCAGCGGAATGGATTGCACCATTCTGATGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCTCAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCTACTCGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTTAAGGTGATTTCAATTTCTGGTCAAAAGTTGAGCCTGTCAATGAGGGATGTTGATCAGCATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCGTCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCTGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTTTCAATACAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACGCAGTATCTTGCAGAAGCTGGGTACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCAATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACGGATGGTATGCTTCTTAGGGAGATTCTGCTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATCCCCGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCGGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTATATGAGAGGATGAAGGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCTCTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAACAACGAGCTGGGCGTGCTGGCCGTACAGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTATTACATTAACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCTCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTAACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCGGGGCCTTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCCTTAGGAGGGCGCAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGACGTTGTGAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGCTGCTAGAAAGGACCCACAGGAGGGGTATAGAACCCTAGTGGAGAACCAGCCAATTTATATCCATCCAAGCAGTGCTCTCTTTCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGATAGTTGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCACGGTGGCTGATCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAACGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCCTGGCGTCTTAGTAAACGGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1197

Amino Acids

136.73

Weight (kDa)

6.5

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 245 - 312 4.3e-11 S1 RNA binding domain
DEAD PF00270 551 - 700 1e-07 DEAD/DEAH box helicase
AAA_22 PF13401 559 - 692 1.6e-06 AAA domain
Helicase_C PF00271 744 - 874 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 936 - 964 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 965 - 1024 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1082 - 1158 2.2e-25 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 741, 2532
AasI GACNNNNNNGTC 1 cut(s) 1015
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1487, 3397
AciI CCGC 7 cut(s) 559, 694, 2056, 2638, 2662, 3424, 3536
AcoI YGGCCR 5 cut(s) 1106, 2586, 2611, 2877, 2919
AcsI RAATTY 4 cut(s) 152, 2235, 2694, 3232
AcuI CTGAAG 4 cut(s) 306, 1667, 1851, 2667
AcyI GRCGYC 1 cut(s) 3571
AfaI GTAC 7 cut(s) 38, 208, 1757, 1783, 1916, 1973, 2617
AhlI ACTAGT 1 cut(s) 2557
AjnI CCWGG 8 cut(s) 1298, 1725, 2425, 2506, 2623, 3015, 3470, 3566
AjuI GAANNNNNNNTTGG 4 cut(s) 797, 829, 1640, 1672
Alw21I GWGCWC 3 cut(s) 281, 1127, 3358
Alw26I GTCTC 7 cut(s) 757, 1061, 1478, 1697, 2053, 3147, 3361
AlwNI CAGNNNCTG 3 cut(s) 1487, 1751, 2624
Ama87I CYCGRG 2 cut(s) 32, 1162
ApeKI GCWGC 8 cut(s) 5, 1364, 1367, 1414, 1417, 1802, 2188, 3281
ApoI RAATTY 4 cut(s) 152, 2235, 2694, 3232
ArsI GACNNNNNNTTYG 4 cut(s) 2052, 2084, 2495, 2527
Asp700I GAANNNNTTC 1 cut(s) 1659
AspA2I CCTAGG 1 cut(s) 2999
AspLEI GCGC 2 cut(s) 3163, 3513
AspS9I GGNCC 6 cut(s) 1037, 1895, 2621, 2929, 3113, 3292
AsuC2I CCSGG 1 cut(s) 2151
AsuHPI GGTGA 9 cut(s) 880, 910, 1119, 1311, 1502, 1712, 2270, 2778, 3442
AvaI CYCGRG 2 cut(s) 32, 1162
AvaII GGWCC 3 cut(s) 1895, 2929, 3292
AvrII CCTAGG 1 cut(s) 2999
AxyI CCTNAGG 1 cut(s) 3152
BaeGI GKGCMC 1 cut(s) 2383
BalI TGGCCA 1 cut(s) 2921
BamHI GGATCC 2 cut(s) 2776, 2893
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 1127, 2836
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1580, 2843
Bbv12I GWGCWC 3 cut(s) 281, 1127, 3358
BbvCI CCTCAGC 1 cut(s) 1239
BbvI GCAGC 7 cut(s) 1354, 1376, 1426, 1429, 1789, 2200, 3268
BceAI ACGGC 1 cut(s) 2598
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 8 cut(s) 1300, 1727, 2427, 2508, 2625, 3017, 3472, 3568
BciVI GTATCC 1 cut(s) 1197
BclI TGATCA 2 cut(s) 946, 3022
BcnI CCSGG 1 cut(s) 2151
BcoDI GTCTC 7 cut(s) 757, 1061, 1478, 1697, 2053, 3147, 3361
BcuI ACTAGT 1 cut(s) 2557
BfmI CTRYAG 4 cut(s) 1308, 1812, 2166, 3261
BfuI GTATCC 1 cut(s) 1197
BisI GCNGC 8 cut(s) 6, 1365, 1368, 1415, 1418, 1803, 2189, 3282
BlnI CCTAGG 1 cut(s) 2999
BlsI GCNGC 8 cut(s) 7, 1366, 1369, 1416, 1419, 1804, 2190, 3283
BmcAI AGTACT 1 cut(s) 1973
Bme1390I CCNGG 9 cut(s) 1300, 1727, 2151, 2427, 2508, 2625, 3017, 3472, 3568
Bme18I GGWCC 3 cut(s) 1895, 2929, 3292
BmeT110I CYCGRG 2 cut(s) 32, 1162
BmgT120I GGNCC 6 cut(s) 1037, 1895, 2621, 2929, 3113, 3292
BmiI GGNNCC 7 cut(s) 181, 2250, 2778, 2833, 2895, 3114, 3294
BmrFI CCNGG 9 cut(s) 1300, 1727, 2151, 2427, 2508, 2625, 3017, 3472, 3568
BmrI ACTGGG 2 cut(s) 966, 2382
BmuI ACTGGG 2 cut(s) 966, 2382
BpiI GAAGAC 2 cut(s) 1580, 2843
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1239
BpuEI CTTGAG 2 cut(s) 1271, 2775
BpuMI CCSGG 1 cut(s) 2151
Bsa29I ATCGAT 2 cut(s) 607, 2481
BsaAI YACGTR 1 cut(s) 1793
BsaBI GATNNNNATC 3 cut(s) 477, 945, 1455
BsaHI GRCGYC 1 cut(s) 3571
BsaI GGTCTC 3 cut(s) 757, 1061, 2053
BsaWI WCCGGW 2 cut(s) 1129, 3373
BsaXI ACNNNNNCTCC 2 cut(s) 667, 697
Bse118I RCCGGY 1 cut(s) 1104
Bse1I ACTGG 9 cut(s) 961, 1492, 1711, 1897, 2308, 2369, 2388, 2922, 3213
Bse21I CCTNAGG 1 cut(s) 3152
Bse3DI GCAATG 4 cut(s) 1812, 2671, 2745, 2961
Bse8I GATNNNNATC 3 cut(s) 477, 945, 1455
BseBI CCWGG 8 cut(s) 1300, 1727, 2427, 2508, 2625, 3017, 3472, 3568
BseCI ATCGAT 2 cut(s) 607, 2481
BseJI GATNNNNATC 3 cut(s) 477, 945, 1455
BseMI GCAATG 4 cut(s) 1812, 2671, 2745, 2961
BseMII CTCAG 8 cut(s) 111, 1002, 1230, 1539, 1982, 2094, 2343, 2640
BseNI ACTGG 9 cut(s) 961, 1492, 1711, 1897, 2308, 2369, 2388, 2922, 3213
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 2383
BseXI GCAGC 7 cut(s) 1354, 1376, 1426, 1429, 1789, 2200, 3268
BseYI CCCAGC 2 cut(s) 1751, 2600
BsgI GTGCAG 3 cut(s) 1353, 1365, 1383
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 2 cut(s) 607, 2481
BsiHKAI GWGCWC 3 cut(s) 281, 1127, 3358
BsiHKCI CYCGRG 2 cut(s) 32, 1162
BsiSI CCGG 5 cut(s) 1105, 1130, 2151, 2252, 3374
BsmAI GTCTC 7 cut(s) 757, 1061, 1478, 1697, 2053, 3147, 3361
Bso31I GGTCTC 3 cut(s) 757, 1061, 2053
BsoBI CYCGRG 2 cut(s) 32, 1162
Bsp1286I GDGCHC 5 cut(s) 281, 1127, 2383, 2836, 3358
Bsp1407I TGTACA 1 cut(s) 1781
BspACI CCGC 7 cut(s) 559, 694, 2056, 2638, 2662, 3424, 3536
BspCNI CTCAG 8 cut(s) 112, 1001, 1231, 1540, 1981, 2095, 2344, 2641
BspDI ATCGAT 2 cut(s) 607, 2481
BspHI TCATGA 2 cut(s) 577, 1999
BspLI GGNNCC 7 cut(s) 181, 2250, 2778, 2833, 2895, 3114, 3294
BspMAI CTGCAG 2 cut(s) 1312, 3265
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 757, 1061, 2053
BsrDI GCAATG 4 cut(s) 1812, 2671, 2745, 2961
BsrFI RCCGGY 1 cut(s) 1104
BsrGI TGTACA 1 cut(s) 1781
BsrI ACTGG 9 cut(s) 961, 1492, 1711, 1897, 2308, 2369, 2388, 2922, 3213
BssAI RCCGGY 1 cut(s) 1104
BssNI GRCGYC 1 cut(s) 3571
BssT1I CCWWGG 8 cut(s) 45, 54, 243, 1463, 2094, 2932, 2999, 3116
Bst2UI CCWGG 8 cut(s) 1300, 1727, 2427, 2508, 2625, 3017, 3472, 3568
Bst4CI ACNGT 9 cut(s) 137, 179, 1152, 1579, 1906, 2132, 2227, 2825, 3484
Bst6I CTCTTC 8 cut(s) 866, 1074, 1109, 1224, 1827, 2426, 2838, 3023
BstACI GRCGYC 1 cut(s) 3571
BstAPI GCANNNNNTGC 2 cut(s) 1630, 3260
BstAUI TGTACA 1 cut(s) 1781
BstBAI YACGTR 1 cut(s) 1793
BstHHI GCGC 2 cut(s) 3163, 3513
BstMAI GTCTC 7 cut(s) 757, 1061, 1478, 1697, 2053, 3147, 3361
BstMWI GCNNNNNNNGC 5 cut(s) 1414, 1630, 2606, 3095, 3260
BstNI CCWGG 8 cut(s) 1300, 1727, 2427, 2508, 2625, 3017, 3472, 3568
BstNSI RCATGY 2 cut(s) 1546, 2307
BstSCI CCNGG 9 cut(s) 1298, 1725, 2149, 2425, 2506, 2623, 3015, 3470, 3566
BstSFI CTRYAG 4 cut(s) 1308, 1812, 2166, 3261
BstSLI GKGCMC 1 cut(s) 2383
BstV1I GCAGC 7 cut(s) 1354, 1376, 1426, 1429, 1789, 2200, 3268
BstV2I GAAGAC 2 cut(s) 1580, 2843
BstX2I RGATCY 5 cut(s) 1450, 1471, 2776, 2893, 2949
BstXI CCANNNNNNTGG 1 cut(s) 3567
BstYI RGATCY 5 cut(s) 1450, 1471, 2776, 2893, 2949
Bsu15I ATCGAT 2 cut(s) 607, 2481
Bsu36I CCTNAGG 1 cut(s) 3152
BsuI GTATCC 1 cut(s) 1197
BsuTUI ATCGAT 2 cut(s) 607, 2481
BtsI GCAGTG 4 cut(s) 279, 1674, 2947, 3358
BtsIMutI CAGTG 8 cut(s) 279, 1674, 1890, 2223, 2395, 2646, 2947, 3358
CaiI CAGNNNCTG 3 cut(s) 1487, 1751, 2624
CciI TCATGA 2 cut(s) 577, 1999
CfoI GCGC 2 cut(s) 3163, 3513
Cfr10I RCCGGY 1 cut(s) 1104
Cfr13I GGNCC 6 cut(s) 1037, 1895, 2621, 2929, 3113, 3292
ClaI ATCGAT 2 cut(s) 607, 2481
CseI GACGC 2 cut(s) 3506, 3560
CsiI ACCWGGT 1 cut(s) 1298
Csp6I GTAC 7 cut(s) 37, 207, 1756, 1782, 1915, 1972, 2616
CviQI GTAC 7 cut(s) 37, 207, 1756, 1782, 1915, 1972, 2616
DrdI GACNNNNNNGTC 1 cut(s) 1015
DseDI GACNNNNNNGTC 1 cut(s) 1015
EaeI YGGCCR 5 cut(s) 1106, 2586, 2611, 2877, 2919
Eam1104I CTCTTC 8 cut(s) 866, 1074, 1109, 1224, 1827, 2426, 2838, 3023
EarI CTCTTC 8 cut(s) 866, 1074, 1109, 1224, 1827, 2426, 2838, 3023
EciI GGCGGA 1 cut(s) 574
Ecl136II GAGCTC 1 cut(s) 1125
Eco130I CCWWGG 8 cut(s) 45, 54, 243, 1463, 2094, 2932, 2999, 3116
Eco147I AGGCCT 2 cut(s) 430, 2998
Eco24I GRGCYC 2 cut(s) 1127, 2836
Eco31I GGTCTC 3 cut(s) 757, 1061, 2053
Eco32I GATATC 2 cut(s) 349, 1023
Eco47I GGWCC 3 cut(s) 1895, 2929, 3292
Eco53kI GAGCTC 1 cut(s) 1125
Eco57I CTGAAG 4 cut(s) 306, 1667, 1851, 2667
Eco81I CCTNAGG 1 cut(s) 3152
Eco88I CYCGRG 2 cut(s) 32, 1162
EcoICRI GAGCTC 1 cut(s) 1125
EcoO109I RGGNCCY 3 cut(s) 2621, 3113, 3292
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 8 cut(s) 1298, 1725, 2425, 2506, 2623, 3015, 3470, 3566
EcoRV GATATC 2 cut(s) 349, 1023
EcoT14I CCWWGG 8 cut(s) 45, 54, 243, 1463, 2094, 2932, 2999, 3116
EcoT22I ATGCAT 1 cut(s) 2215
EcoT38I GRGCYC 2 cut(s) 1127, 2836
ErhI CCWWGG 8 cut(s) 45, 54, 243, 1463, 2094, 2932, 2999, 3116
FalI AAGNNNNNCTT 2 cut(s) 2835, 2867
FauI CCCGC 1 cut(s) 3417
FbaI TGATCA 2 cut(s) 946, 3022
Fnu4HI GCNGC 8 cut(s) 6, 1365, 1368, 1415, 1418, 1803, 2189, 3282
FriOI GRGCYC 2 cut(s) 1127, 2836
Fsp4HI GCNGC 8 cut(s) 6, 1365, 1368, 1415, 1418, 1803, 2189, 3282
GlaI GCGC 2 cut(s) 3162, 3512
GluI GCNGC 8 cut(s) 6, 1365, 1368, 1415, 1418, 1803, 2189, 3282
GsaI CCCAGC 2 cut(s) 1755, 2604
HapII CCGG 5 cut(s) 1105, 1130, 2151, 2252, 3374
HgaI GACGC 2 cut(s) 3506, 3560
HhaI GCGC 2 cut(s) 3163, 3513
Hin1I GRCGYC 1 cut(s) 3571
Hin6I GCGC 2 cut(s) 3161, 3511
HinP1I GCGC 2 cut(s) 3161, 3511
HincII GTYRAC 1 cut(s) 3442
HindII GTYRAC 1 cut(s) 3442
HindIII AAGCTT 1 cut(s) 1601
HinfI GANTC 9 cut(s) 263, 998, 1339, 1357, 1436, 1456, 1945, 2552, 2971
HpaII CCGG 5 cut(s) 1105, 1130, 2151, 2252, 3374
HphI GGTGA 9 cut(s) 880, 910, 1119, 1311, 1502, 1712, 2270, 2778, 3442
Hpy166II GTNNAC 7 cut(s) 209, 1758, 1782, 1784, 2929, 3442, 3581
Hpy8I GTNNAC 7 cut(s) 209, 1758, 1782, 1784, 2929, 3442, 3581
Hpy99I CGWCG 1 cut(s) 1021
HpyCH4III ACNGT 9 cut(s) 137, 179, 1152, 1579, 1906, 2132, 2227, 2825, 3484
HpyCH4IV ACGT 3 cut(s) 1792, 3213, 3526
HpyF10VI GCNNNNNNNGC 5 cut(s) 1414, 1630, 2606, 3095, 3260
HpySE526I ACGT 3 cut(s) 1792, 3213, 3526
Hsp92I GRCGYC 1 cut(s) 3571
HspAI GCGC 2 cut(s) 3161, 3511
KroI GCCGGC 1 cut(s) 1104
KroNI GCCGGC 1 cut(s) 1106
Ksp22I TGATCA 2 cut(s) 946, 3022
LmnI GCTCC 4 cut(s) 1235, 1393, 2248, 2831
Lsp1109I GCAGC 7 cut(s) 1354, 1376, 1426, 1429, 1789, 2200, 3268
MabI ACCWGGT 1 cut(s) 1298
MaeII ACGT 3 cut(s) 1792, 3213, 3526
MflI RGATCY 5 cut(s) 1450, 1471, 2776, 2893, 2949
MhlI GDGCHC 5 cut(s) 281, 1127, 2383, 2836, 3358
MlsI TGGCCA 1 cut(s) 2921
MluNI TGGCCA 1 cut(s) 2921
MlyI GAGTC 2 cut(s) 257, 1366
MmeI TCCRAC 3 cut(s) 348, 1413, 1843
Mox20I TGGCCA 1 cut(s) 2921
Mph1103I ATGCAT 1 cut(s) 2215
MroNI GCCGGC 1 cut(s) 1104
MroXI GAANNNNTTC 1 cut(s) 1659
MscI TGGCCA 1 cut(s) 2921
MseI TTAA 7 cut(s) 894, 1263, 1335, 1389, 2219, 2243, 2726
MslI CAYNNNNRTG 7 cut(s) 414, 576, 582, 714, 1763, 2019, 2966
Msp20I TGGCCA 1 cut(s) 2921
MspA1I CMGCKG 2 cut(s) 694, 1367
MspI CCGG 5 cut(s) 1105, 1130, 2151, 2252, 3374
MspR9I CCNGG 9 cut(s) 1300, 1727, 2151, 2427, 2508, 2625, 3017, 3472, 3568
MvaI CCWGG 8 cut(s) 1300, 1727, 2427, 2508, 2625, 3017, 3472, 3568
MwoI GCNNNNNNNGC 5 cut(s) 1414, 1630, 2606, 3095, 3260
NaeI GCCGGC 1 cut(s) 1106
NciI CCSGG 1 cut(s) 2151
NgoMIV GCCGGC 1 cut(s) 1104
NlaIV GGNNCC 7 cut(s) 181, 2250, 2778, 2833, 2895, 3114, 3294
NmeAIII GCCGAG 5 cut(s) 133, 208, 820, 2488, 2905
NmuCI GTSAC 6 cut(s) 1042, 1317, 1579, 2080, 2258, 2310
NsiI ATGCAT 1 cut(s) 2215
NspI RCATGY 2 cut(s) 1546, 2307
PaeI GCATGC 1 cut(s) 2307
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 577, 1999
PceI AGGCCT 2 cut(s) 430, 2998
PcsI WCGNNNNNNNCGW 1 cut(s) 2602
PdiI GCCGGC 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 1659
PfeI GAWTC 7 cut(s) 998, 1339, 1436, 1456, 1945, 2552, 2971
PflMI CCANNNNNTGG 2 cut(s) 1487, 3397
PkrI GCNGC 8 cut(s) 7, 1366, 1369, 1416, 1419, 1804, 2190, 3283
PleI GAGTC 2 cut(s) 257, 1365
PpsI GAGTC 2 cut(s) 257, 1365
Ppu21I YACGTR 1 cut(s) 1793
PpuMI RGGWCCY 1 cut(s) 3292
PsiI TTATAA 2 cut(s) 741, 2532
Psp124BI GAGCTC 1 cut(s) 1127
Psp5II RGGWCCY 1 cut(s) 3292
Psp6I CCWGG 8 cut(s) 1298, 1725, 2425, 2506, 2623, 3015, 3470, 3566
PspFI CCCAGC 2 cut(s) 1751, 2600
PspGI CCWGG 8 cut(s) 1298, 1725, 2425, 2506, 2623, 3015, 3470, 3566
PspN4I GGNNCC 7 cut(s) 181, 2250, 2778, 2833, 2895, 3114, 3294
PspPI GGNCC 6 cut(s) 1037, 1895, 2621, 2929, 3113, 3292
PspPPI RGGWCCY 1 cut(s) 3292
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 2 cut(s) 1312, 3265
PstNI CAGNNNCTG 3 cut(s) 1487, 1751, 2624
PsuI RGATCY 5 cut(s) 1450, 1471, 2776, 2893, 2949
PvuII CAGCTG 1 cut(s) 1367
RsaI GTAC 7 cut(s) 38, 208, 1757, 1783, 1916, 1973, 2617
RsaNI GTAC 7 cut(s) 37, 207, 1756, 1782, 1915, 1972, 2616
RseI CAYNNNNRTG 7 cut(s) 414, 576, 582, 714, 1763, 2019, 2966
SacI GAGCTC 1 cut(s) 1127
SaqAI TTAA 7 cut(s) 894, 1263, 1335, 1389, 2219, 2243, 2726
SatI GCNGC 8 cut(s) 6, 1365, 1368, 1415, 1418, 1803, 2189, 3282
Sau96I GGNCC 6 cut(s) 1037, 1895, 2621, 2929, 3113, 3292
ScaI AGTACT 1 cut(s) 1973
SchI GAGTC 2 cut(s) 257, 1366
ScrFI CCNGG 9 cut(s) 1300, 1727, 2151, 2427, 2508, 2625, 3017, 3472, 3568
SduI GDGCHC 5 cut(s) 281, 1127, 2383, 2836, 3358
SexAI ACCWGGT 1 cut(s) 1298
SfcI CTRYAG 4 cut(s) 1308, 1812, 2166, 3261
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 3 cut(s) 1895, 2929, 3292
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 7 cut(s) 414, 576, 582, 714, 1763, 2019, 2966
SmlI CTYRAG 3 cut(s) 32, 1250, 2790
SmoI CTYRAG 3 cut(s) 32, 1250, 2790
SpeI ACTAGT 1 cut(s) 2557
SphI GCATGC 1 cut(s) 2307
SseBI AGGCCT 2 cut(s) 430, 2998
SsiI CCGC 7 cut(s) 559, 694, 2056, 2638, 2662, 3424, 3536
SspI AATATT 1 cut(s) 1332
SstI GAGCTC 1 cut(s) 1127
StuI AGGCCT 2 cut(s) 430, 2998
StyD4I CCNGG 9 cut(s) 1298, 1725, 2149, 2425, 2506, 2623, 3015, 3470, 3566
StyI CCWWGG 8 cut(s) 45, 54, 243, 1463, 2094, 2932, 2999, 3116
TaaI ACNGT 9 cut(s) 137, 179, 1152, 1579, 1906, 2132, 2227, 2825, 3484
TaiI ACGT 3 cut(s) 1795, 3216, 3529
TaqI TCGA 8 cut(s) 33, 156, 607, 1019, 2127, 2402, 2481, 3145
TatI WGTACW 3 cut(s) 1781, 1914, 1971
TfiI GAWTC 7 cut(s) 998, 1339, 1436, 1456, 1945, 2552, 2971
Tru1I TTAA 7 cut(s) 894, 1263, 1335, 1389, 2219, 2243, 2726
Tru9I TTAA 7 cut(s) 894, 1263, 1335, 1389, 2219, 2243, 2726
TscAI CASTG 8 cut(s) 286, 1674, 1897, 2230, 2395, 2653, 2947, 3358
TseFI GTSAC 6 cut(s) 1042, 1317, 1579, 2080, 2258, 2310
TseI GCWGC 8 cut(s) 5, 1364, 1367, 1414, 1417, 1802, 2188, 3281
Tsp45I GTSAC 6 cut(s) 1042, 1317, 1579, 2080, 2258, 2310
TspGWI ACGGA 3 cut(s) 194, 1937, 2261
TspRI CASTG 8 cut(s) 286, 1674, 1897, 2230, 2395, 2653, 2947, 3358
Van91I CCANNNNNTGG 2 cut(s) 1487, 3397
VpaK11BI GGWCC 3 cut(s) 1895, 2929, 3292
XapI RAATTY 4 cut(s) 152, 2235, 2694, 3232
XceI RCATGY 2 cut(s) 1546, 2307
XcmI CCANNNNNNNNNTGG 1 cut(s) 3454
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2999
XmnI GAANNNNTTC 1 cut(s) 1659
ZrmI AGTACT 1 cut(s) 1973
Zsp2I ATGCAT 1 cut(s) 2215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.