Rw5G049150

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000671
Physical Location & Seq
Forward (+)
85246781 .. 85250012
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049150.1

Sequence Viewer

Length: 3102 bp
ATGGCCGTCAGTGTCAAGAAGCTAGAGTACCTTTCCTTGATTTCAAAAGTCTGTTCGGAACTCGAAACCAATCTAGGGCAGCAGTATGGGGACAAAGCCCTAGCTGAGTTCATAACCGATATGGGACGGAACTCCAAGAACGTGGACGAATTCAATGCCAAGTTGAATGAATTTGGCGCCGAAATCCCTGATTACTTTGTCCGTAATCTTCTCACTATTATTCACGCAGTTCTTGCTCCCAACAAGAACCAGAGCTCTTCTAAAGAAGAAAAAGAAGACAAGCCTAGAGATAGAAGTAGAGAAAGCAGAGGTAAGAATAAGGATCAAGAGTACAATTCCGGTGAGCCGGAGTTGTATGGAGTTTATAAGGGCAGGGTTTCGAAAGTGATGGATGCAGGTTGCTTTGTTGAGTTGAGTTGTTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCGAACAGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAGCAAGTTTATGTGAAGGTGGTTTCGATTTCGGGTCAGAAGTTGAGTCTTTCAATGAAGGATGTTGATCAGAAGACAGGGAAGGATTTACTTCCTTTGAAGAAGAGCTCAGATTATGATGCTTGTAGGTCGAACCCGTCAATGTCAAAACATGGTTCTGTGAGTAGGACTAGTCTTTCTGGGATTAGGATTGTGGAGGAGGATAGTTTTGTCCAATCTCGTAGGCCAAGGAAGAGAATGAGCTCCCCAGAGAGGTGGGAAATCAAACAGTTGATTGCTTCAGGTGAAGTGGATGGCATAACGATGAAGCAGCTCTATAAAGATGAGGGACCTGAGGAAGAGCTTGAAATCGAGCTCAATGAGGAAGCACCGGCCTTTTTGCAAGGGCACAATATGTATTCTGTGGATATGTCGCCGGTGAAGATCTTTAAGAATCCAGAAGGGTCACTGAGCCGTGCAGCTGCACTTCAATCTGCACTGATCAAGGAGCGCCGAGAAATGAGAGATCAGCAGCAAAGAGCAGTGCTGGATTCCATCCCTAAGGACCTGAATCGTCCGTGGGAAGATCCAATGGCACAGAATGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCAGCTTATGACATGCCTGAGTGGAAGAAGGATACTTATGCGAAAGCTATGAGTTTCGGGAAGAGGTCAAGCCTCTCGATTCAGGAGCAAAGGAAGAGCTTGCCTGTCTACAAGCTGAGGGAAGAACTGATTAAGGCAGTGAATGAGAATCAAGTGCTTATTGTCATTGGTGAGACTGGTTCTGGTAAGACAACTCAGCTAACACAGTATCTTGCAGAAGCAGGTTACACGACAAAGGGTAGGATTGGATGTACACAGCCTCGTAGGGTGGCAGCAATGTCAGTGGCGAAAAGGGTTGCTGAAGAGGTTGGTTGTCGGTTGGGGGAGGAAGTAGGTTATGCCATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACAGATGGTATGCTTCTTAGGGAGATTTTGATGGATGAGAACCTTTCGCAATACTCTGTGATAATGCTCGATGAAGCTCATGAGAGGACAATCCACACGGATGTTCTTTTTGGATTACTGAAGCAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTTACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCAACTGCATGCTCTTCTCTATCCCTGGGAGAACTTTTCCATATAAGGTAAACTATGTCAAGGAGCCAGAAAGTGATTACCTTGATGCGGCTCTAATCACTGTTTTACAGATCCACTTGGAAGAACCTGAGGGTGACATCCTTCTCTTCTTGACTGGACAGGAAGAGATTGATTTTGCATGTCAGATTCTTTATGAGAGGATGAAAGCTCTTGGTAAAGATGTACCTGAGTTGATTATCCTACCAGTTTATAGTGCTCTTCCTAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCGCCGGGGAAGAGGAAAGTAGTTGTGGCTACTAACATTGCCGAGGCATCTCTGACAATTGATGGAATATTTTATGTCGTTGATCCTGGATTTGCAAAACAAAATGTTTATAACCCAAAGCTGGGGCTAGATTCACTGGTCATAACTCCGATTTCACAAGCATCAGCGAAGCAACGAGCTGGGCGTGCTGGGCGTACAGGCCCTGGGAAATGTTACCGTCTCTACACAGAGAGTGCATTCCGCAATGAGATGCCCCCGACTACAATTCCCGAAATCCAGAGGACAAATCTTGGGTCTACGGTGCTTGCAATGAAAGCAATGGGGATAACAATGGAACAACTGTACAGCTTAGGAGCACTTGATGAAGAGGGTTTCCTAACCAGACTAGGAAGGAAAATGTCAGAGTTTCCTCTGGAGCCACCCCTCTCGAAGATGCTACTCGCCAGTGTGGACCTCGGATGCAGTGATGAGATCCTGACCATCATAGCAATGATTCAGACAGGCAATGTCTTTTACAGGCCTAGGGAGAAACAAGACCGGGCAGATCAGAAGAGAGCAAAGTTTTTCCAGCCAGAAGGAGACCATCTTACTCTACTTGAAGTCTACAAGGCTTGGAAGAGAACAAACTTTTCAGCTCCATGGTGTTTCGACAACTTTCTTCAGTCTCGATCCTTGAGGCGCGCACAGGATCACAAGCTGGATATTGTCAGTGCCGGAAACAATTACACAAAGATCAGCAAGGCAATTACGGCCGGCTTTTTCTTCCATGTTGCTAAAAAGGATCCACAAGAAGGTTACAAGACCCTAGTTGAGAGCCAGCCAGTGTACATCCATCCAAGCAGTGCTCTCTTCCAGAAGCAACCAGATTGGGTCATCTACAATGAGCTGGTTATGACTACAAGGGAGTACATGCGCGAGGTGACAGCCATTGATCCCAAATGGCTTGTGGAATTGGCACCAAGGTTCTTCAAAGCTGCAGATCCTAACAAGATGACCAAGCGCAAGCGTCAACAACGTATTGAACCATTGTATGACAGATACAATGAACCCAACTCTTGGCGTCTTACTAGACGCCGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1033

Amino Acids

117.17

Weight (kDa)

8.67

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 121 - 187 4.2e-11 S1 RNA binding domain
DEAD PF00270 417 - 566 3.5e-08 DEAD/DEAH box helicase
AAA_22 PF13401 426 - 557 3.2e-06 AAA domain
Helicase_C PF00271 609 - 740 9.9e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 784 - 811 3.6e-10 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 812 - 871 7.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 918 - 994 6.7e-27 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 366, 2130
Acc36I ACCTGC 2 cut(s) 386, 1340
AccB1I GGYRCC 2 cut(s) 176, 2974
AccB7I CCANNNNNTGG 1 cut(s) 3075
AccI GTMKAC 3 cut(s) 1236, 2315, 2622
AccII CGCG 2 cut(s) 2700, 2934
AciI CCGC 4 cut(s) 1654, 1811, 2021, 2260
AcoI YGGCCR 2 cut(s) 3, 2769
AcsI RAATTY 2 cut(s) 149, 170
AcuI CTGAAG 4 cut(s) 768, 1449, 1658, 2663
AcyI GRCGYC 3 cut(s) 177, 3079, 3091
AfaI GTAC 9 cut(s) 29, 332, 1381, 1514, 1947, 2215, 2363, 2846, 2927
AfiI CCNNNNNNNGG 8 cut(s) 75, 756, 1810, 2025, 2140, 2141, 2810, 3075
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 3 cut(s) 1747, 2104, 2221
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
Alw21I GWGCWC 7 cut(s) 257, 614, 749, 861, 1981, 2377, 2866
Alw26I GTCTC 5 cut(s) 1295, 1651, 2243, 2592, 2688
AlwNI CAGNNNCTG 2 cut(s) 1502, 2222
AoxI GGCC 6 cut(s) 3, 728, 876, 2218, 2537, 2769
ApeKI GCWGC 7 cut(s) 79, 814, 962, 965, 1015, 1400, 2993
ApoI RAATTY 2 cut(s) 149, 170
ArsI GACNNNNNNTTYG 4 cut(s) 1650, 1682, 2297, 2329
AspA2I CCTAGG 1 cut(s) 2540
AspLEI GCGC 6 cut(s) 179, 996, 2700, 2702, 2934, 3021
AspS9I GGNCC 5 cut(s) 833, 1048, 1493, 2219, 2470
AsuC2I CCSGG 2 cut(s) 2025, 2558
AsuHPI GGTGA 8 cut(s) 353, 505, 800, 934, 1100, 1310, 1868, 2950
AsuII TTCGAA 1 cut(s) 380
AvaII GGWCC 4 cut(s) 833, 1048, 1493, 2470
AvrII CCTAGG 1 cut(s) 2540
AxyI CCTNAGG 3 cut(s) 837, 1044, 1851
BaeGI GKGCMC 1 cut(s) 894
BamHI GGATCC 1 cut(s) 2800
BanI GGYRCC 2 cut(s) 176, 2974
BanII GRGCYC 4 cut(s) 257, 614, 749, 861
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BbsI GAAGAC 2 cut(s) 282, 584
Bbv12I GWGCWC 7 cut(s) 257, 614, 749, 861, 1981, 2377, 2866
BbvCI CCTCAGC 1 cut(s) 1244
BbvI GCAGC 7 cut(s) 91, 826, 952, 974, 1027, 1412, 2980
BccI CCATC 9 cut(s) 382, 791, 1046, 1517, 1543, 2075, 2507, 2610, 2859
BceAI ACGGC 3 cut(s) 942, 2784, 3078
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 3 cut(s) 1749, 2106, 2223
BciVI GTATCC 1 cut(s) 1153
BclI TGATCA 2 cut(s) 571, 984
BcnI CCSGG 2 cut(s) 2025, 2558
BcoDI GTCTC 5 cut(s) 1295, 1651, 2243, 2592, 2688
BcuI ACTAGT 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 2994
BfoI RGCGCY 2 cut(s) 180, 997
BfuAI ACCTGC 2 cut(s) 386, 1340
BfuI GTATCC 1 cut(s) 1153
BglII AGATCT 1 cut(s) 927
BisI GCNGC 8 cut(s) 80, 815, 963, 966, 1016, 1401, 1812, 2994
BlnI CCTAGG 1 cut(s) 2540
BlsI GCNGC 8 cut(s) 81, 816, 964, 967, 1017, 1402, 1813, 2995
Bme1390I CCNGG 5 cut(s) 1749, 2025, 2106, 2223, 2558
Bme18I GGWCC 4 cut(s) 833, 1048, 1493, 2470
BmgT120I GGNCC 5 cut(s) 833, 1048, 1493, 2219, 2470
BmiI GGNNCC 6 cut(s) 178, 834, 1788, 2436, 2802, 2976
BmrFI CCNGG 5 cut(s) 1749, 2025, 2106, 2223, 2558
BpiI GAAGAC 2 cut(s) 282, 584
BpmI CTGGAG 1 cut(s) 2453
Bpu10I CCTNAGC 2 cut(s) 1244, 2368
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 2713
BpuMI CCSGG 2 cut(s) 2025, 2558
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 3 cut(s) 177, 3079, 3091
BsaI GGTCTC 2 cut(s) 1651, 2592
BsaWI WCCGGW 1 cut(s) 338
Bsc4I CCNNNNNNNGG 8 cut(s) 75, 756, 1810, 2025, 2140, 2141, 2810, 3075
Bse118I RCCGGY 3 cut(s) 874, 919, 2771
Bse1I ACTGG 7 cut(s) 1309, 1495, 1882, 1967, 2160, 2463, 2840
Bse21I CCTNAGG 3 cut(s) 837, 1044, 1851
Bse3DI GCAATG 7 cut(s) 1410, 2055, 2269, 2334, 2343, 2514, 2530
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 3 cut(s) 1749, 2106, 2223
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 8 cut(s) 75, 756, 1810, 2025, 2140, 2141, 2810, 3075
BseMI GCAATG 7 cut(s) 1410, 2055, 2269, 2334, 2343, 2514, 2530
BseMII CTCAG 9 cut(s) 96, 627, 828, 944, 1137, 1235, 1337, 1842, 1941
BseNI ACTGG 7 cut(s) 1309, 1495, 1882, 1967, 2160, 2463, 2840
BsePI GCGCGC 1 cut(s) 2698
BseRI GAGGAG 1 cut(s) 716
BseSI GKGCMC 1 cut(s) 894
BseX3I CGGCCG 1 cut(s) 2769
BseXI GCAGC 7 cut(s) 91, 826, 952, 974, 1027, 1412, 2980
BseYI CCCAGC 3 cut(s) 2140, 2198, 2207
BsgI GTGCAG 3 cut(s) 951, 963, 981
Bsh1236I CGCG 2 cut(s) 2700, 2934
Bsh1285I CGRYCG 1 cut(s) 2772
BshFI GGCC 6 cut(s) 5, 730, 878, 2220, 2539, 2771
BshNI GGYRCC 2 cut(s) 176, 2974
BsiEI CGRYCG 1 cut(s) 2772
BsiHKAI GWGCWC 7 cut(s) 257, 614, 749, 861, 1981, 2377, 2866
BsiSI CCGG 8 cut(s) 339, 347, 875, 920, 2024, 2557, 2733, 2772
BslFI GGGAC 3 cut(s) 104, 138, 846
BslI CCNNNNNNNGG 8 cut(s) 75, 756, 1810, 2025, 2140, 2141, 2810, 3075
BsmAI GTCTC 5 cut(s) 1295, 1651, 2243, 2592, 2688
BsmBI CGTCTC 1 cut(s) 2243
BsmFI GGGAC 3 cut(s) 104, 138, 846
BsmI GAATGC 1 cut(s) 2255
BsnI GGCC 6 cut(s) 5, 730, 878, 2220, 2539, 2771
Bso31I GGTCTC 2 cut(s) 1651, 2592
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 8 cut(s) 257, 614, 749, 861, 894, 1981, 2377, 2866
Bsp1407I TGTACA 3 cut(s) 1379, 2361, 2844
Bsp19I CCATGG 1 cut(s) 2657
BspACI CCGC 4 cut(s) 1654, 1811, 2021, 2260
BspANI GGCC 6 cut(s) 5, 730, 878, 2220, 2539, 2771
BspCNI CTCAG 9 cut(s) 97, 626, 829, 945, 1138, 1236, 1336, 1843, 1942
BspFNI CGCG 2 cut(s) 2700, 2934
BspHI TCATGA 1 cut(s) 1597
BspLI GGNNCC 6 cut(s) 178, 834, 1788, 2436, 2802, 2976
BspMAI CTGCAG 1 cut(s) 2998
BspMI ACCTGC 2 cut(s) 386, 1340
BspQI GCTCTTC 6 cut(s) 262, 602, 837, 1217, 1742, 1986
BspT104I TTCGAA 1 cut(s) 380
BspT107I GGYRCC 2 cut(s) 176, 2974
BspTNI GGTCTC 2 cut(s) 1651, 2592
BsrDI GCAATG 7 cut(s) 1410, 2055, 2269, 2334, 2343, 2514, 2530
BsrFI RCCGGY 3 cut(s) 874, 919, 2771
BsrGI TGTACA 3 cut(s) 1379, 2361, 2844
BsrI ACTGG 7 cut(s) 1309, 1495, 1882, 1967, 2160, 2463, 2840
BssAI RCCGGY 3 cut(s) 874, 919, 2771
BssHII GCGCGC 1 cut(s) 2698
BssNI GRCGYC 3 cut(s) 177, 3079, 3091
BssT1I CCWWGG 4 cut(s) 731, 2540, 2657, 2978
Bst2UI CCWGG 3 cut(s) 1749, 2106, 2223
Bst4CI ACNGT 7 cut(s) 774, 1335, 1504, 1825, 2237, 2320, 2361
BstACI GRCGYC 3 cut(s) 177, 3079, 3091
BstAPI GCANNNNNTGC 1 cut(s) 233
BstAUI TGTACA 3 cut(s) 1379, 2361, 2844
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 8 cut(s) 1229, 1733, 2205, 2325, 2700, 2773, 2837, 3023
BstDSI CCRYGG 3 cut(s) 1061, 2657, 3094
BstFNI CGCG 2 cut(s) 2700, 2934
BstH2I RGCGCY 2 cut(s) 180, 997
BstHHI GCGC 6 cut(s) 179, 996, 2700, 2702, 2934, 3021
BstMAI GTCTC 5 cut(s) 1295, 1651, 2243, 2592, 2688
BstMCI CGRYCG 1 cut(s) 2772
BstMWI GCNNNNNNNGC 5 cut(s) 233, 2204, 2209, 2333, 2768
BstNI CCWGG 3 cut(s) 1749, 2106, 2223
BstNSI RCATGY 4 cut(s) 1144, 1735, 1905, 2932
BstSCI CCNGG 5 cut(s) 1747, 2023, 2104, 2221, 2556
BstSFI CTRYAG 1 cut(s) 2994
BstSLI GKGCMC 1 cut(s) 894
BstUI CGCG 2 cut(s) 2700, 2934
BstV1I GCAGC 7 cut(s) 91, 826, 952, 974, 1027, 1412, 2980
BstV2I GAAGAC 2 cut(s) 282, 584
BstX2I RGATCY 6 cut(s) 927, 1069, 1833, 2490, 2800, 2998
BstXI CCANNNNNNTGG 2 cut(s) 142, 759
BstYI RGATCY 6 cut(s) 927, 1069, 1833, 2490, 2800, 2998
BstZI CGGCCG 1 cut(s) 2769
Bsu36I CCTNAGG 3 cut(s) 837, 1044, 1851
BsuI GTATCC 1 cut(s) 1153
BsuRI GGCC 6 cut(s) 5, 730, 878, 2220, 2539, 2771
BtgI CCRYGG 3 cut(s) 1061, 2657, 3094
BtsI GCAGTG 4 cut(s) 1032, 1272, 2488, 2866
BveI ACCTGC 2 cut(s) 386, 1340
Cac8I GCNNGC 8 cut(s) 1229, 1733, 2205, 2325, 2700, 2773, 2837, 3023
CaiI CAGNNNCTG 2 cut(s) 1502, 2222
CciI TCATGA 1 cut(s) 1597
CfoI GCGC 6 cut(s) 179, 996, 2700, 2702, 2934, 3021
Cfr10I RCCGGY 3 cut(s) 874, 919, 2771
Cfr13I GGNCC 5 cut(s) 833, 1048, 1493, 2219, 2470
CseI GACGC 2 cut(s) 3014, 3068
Csp6I GTAC 9 cut(s) 28, 331, 1380, 1513, 1946, 2214, 2362, 2845, 2926
CspCI CAANNNNNGTGG 2 cut(s) 1392, 1427
CviQI GTAC 9 cut(s) 28, 331, 1380, 1513, 1946, 2214, 2362, 2845, 2926
DinI GGCGCC 1 cut(s) 178
EaeI YGGCCR 2 cut(s) 3, 2769
EagI CGGCCG 1 cut(s) 2769
Ecl136II GAGCTC 4 cut(s) 255, 612, 747, 859
EclXI CGGCCG 1 cut(s) 2769
Eco130I CCWWGG 4 cut(s) 731, 2540, 2657, 2978
Eco147I AGGCCT 1 cut(s) 2539
Eco24I GRGCYC 4 cut(s) 257, 614, 749, 861
Eco31I GGTCTC 2 cut(s) 1651, 2592
Eco47I GGWCC 4 cut(s) 833, 1048, 1493, 2470
Eco52I CGGCCG 1 cut(s) 2769
Eco53kI GAGCTC 4 cut(s) 255, 612, 747, 859
Eco57I CTGAAG 4 cut(s) 768, 1449, 1658, 2663
Eco81I CCTNAGG 3 cut(s) 837, 1044, 1851
EcoICRI GAGCTC 4 cut(s) 255, 612, 747, 859
EcoO109I RGGNCCY 3 cut(s) 833, 1048, 2219
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 1747, 2104, 2221
EcoT14I CCWWGG 4 cut(s) 731, 2540, 2657, 2978
EcoT38I GRGCYC 4 cut(s) 257, 614, 749, 861
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 4 cut(s) 731, 2540, 2657, 2978
Esp3I CGTCTC 1 cut(s) 2243
FaqI GGGAC 3 cut(s) 104, 138, 846
FauI CCCGC 1 cut(s) 2028
FbaI TGATCA 2 cut(s) 571, 984
FblI GTMKAC 3 cut(s) 1236, 2315, 2622
Fnu4HI GCNGC 8 cut(s) 80, 815, 963, 966, 1016, 1401, 1812, 2994
FriOI GRGCYC 4 cut(s) 257, 614, 749, 861
Fsp4HI GCNGC 8 cut(s) 80, 815, 963, 966, 1016, 1401, 1812, 2994
GlaI GCGC 6 cut(s) 178, 995, 2699, 2701, 2933, 3020
GluI GCNGC 8 cut(s) 80, 815, 963, 966, 1016, 1401, 1812, 2994
GsaI CCCAGC 3 cut(s) 2144, 2202, 2211
GsuI CTGGAG 1 cut(s) 2453
HaeII RGCGCY 2 cut(s) 180, 997
HaeIII GGCC 6 cut(s) 5, 730, 878, 2220, 2539, 2771
HapII CCGG 8 cut(s) 339, 347, 875, 920, 2024, 2557, 2733, 2772
HgaI GACGC 2 cut(s) 3014, 3068
HhaI GCGC 6 cut(s) 179, 996, 2700, 2702, 2934, 3021
Hin1I GRCGYC 3 cut(s) 177, 3079, 3091
Hin6I GCGC 6 cut(s) 177, 994, 2698, 2700, 2932, 3019
HinP1I GCGC 6 cut(s) 177, 994, 2698, 2700, 2932, 3019
HincII GTYRAC 1 cut(s) 3029
HindII GTYRAC 1 cut(s) 3029
HinfI GANTC 9 cut(s) 550, 937, 1034, 1054, 1207, 1276, 1909, 2150, 2512
HpaII CCGG 8 cut(s) 339, 347, 875, 920, 2024, 2557, 2733, 2772
HphI GGTGA 8 cut(s) 353, 505, 800, 934, 1100, 1310, 1868, 2950
Hpy166II GTNNAC 9 cut(s) 145, 1237, 1382, 1774, 2316, 2470, 2623, 2845, 3029
Hpy8I GTNNAC 9 cut(s) 145, 1237, 1382, 1774, 2316, 2470, 2623, 2845, 3029
HpyCH4III ACNGT 7 cut(s) 774, 1335, 1504, 1825, 2237, 2320, 2361
HpyCH4IV ACGT 2 cut(s) 141, 3034
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 2204, 2209, 2333, 2768
HpySE526I ACGT 2 cut(s) 141, 3034
Hsp92I GRCGYC 3 cut(s) 177, 3079, 3091
HspAI GCGC 6 cut(s) 177, 994, 2698, 2700, 2932, 3019
KasI GGCGCC 1 cut(s) 176
KroI GCCGGC 1 cut(s) 2771
KroNI GCCGGC 1 cut(s) 2773
Ksp22I TGATCA 2 cut(s) 571, 984
LguI GCTCTTC 6 cut(s) 262, 602, 837, 1217, 1742, 1986
LmnI GCTCC 8 cut(s) 241, 752, 991, 1213, 1786, 2372, 2434, 2659
Lsp1109I GCAGC 7 cut(s) 91, 826, 952, 974, 1027, 1412, 2980
MaeII ACGT 2 cut(s) 141, 3034
MaeIII GTNAC 7 cut(s) 948, 1352, 1678, 1856, 2231, 2813, 2938
MfeI CAATTG 1 cut(s) 2076
MflI RGATCY 6 cut(s) 927, 1069, 1833, 2490, 2800, 2998
MhlI GDGCHC 8 cut(s) 257, 614, 749, 861, 894, 1981, 2377, 2866
MluCI AATT 9 cut(s) 149, 170, 334, 471, 2076, 2283, 2740, 2763, 2969
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 2771
MseI TTAA 2 cut(s) 933, 1260
MslI CAYNNNNRTG 3 cut(s) 806, 1617, 2507
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 8 cut(s) 339, 347, 875, 920, 2024, 2557, 2733, 2772
MspR9I CCNGG 5 cut(s) 1749, 2025, 2106, 2223, 2558
MunI CAATTG 1 cut(s) 2076
Mva1269I GAATGC 1 cut(s) 2255
MvaI CCWGG 3 cut(s) 1749, 2106, 2223
MvnI CGCG 2 cut(s) 2700, 2934
MwoI GCNNNNNNNGC 5 cut(s) 233, 2204, 2209, 2333, 2768
NaeI GCCGGC 1 cut(s) 2773
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 2025, 2558
NcoI CCATGG 1 cut(s) 2657
NgoMIV GCCGGC 1 cut(s) 2771
NlaIV GGNNCC 6 cut(s) 178, 834, 1788, 2436, 2802, 2976
NmeAIII GCCGAG 2 cut(s) 1022, 2086
NmuCI GTSAC 3 cut(s) 948, 1856, 2938
NspI RCATGY 4 cut(s) 1144, 1735, 1905, 2932
NspV TTCGAA 1 cut(s) 380
PaeI GCATGC 1 cut(s) 1735
PagI TCATGA 1 cut(s) 1597
PasI CCCWGGG 2 cut(s) 1748, 2222
PauI GCGCGC 1 cut(s) 2698
PceI AGGCCT 1 cut(s) 2539
PciSI GCTCTTC 6 cut(s) 262, 602, 837, 1217, 1742, 1986
PcsI WCGNNNNNNNCGW 2 cut(s) 458, 2200
PctI GAATGC 1 cut(s) 2255
PdiI GCCGGC 1 cut(s) 2773
PfeI GAWTC 8 cut(s) 937, 1034, 1054, 1207, 1276, 1909, 2150, 2512
PflMI CCANNNNNTGG 1 cut(s) 3075
PfoI TCCNGGA 1 cut(s) 2104
PkrI GCNGC 8 cut(s) 81, 816, 964, 967, 1017, 1402, 1813, 2995
PleI GAGTC 1 cut(s) 558
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 558
PpuMI RGGWCCY 2 cut(s) 833, 1048
PsiI TTATAA 2 cut(s) 366, 2130
Psp124BI GAGCTC 4 cut(s) 257, 614, 749, 861
Psp5II RGGWCCY 2 cut(s) 833, 1048
Psp6I CCWGG 3 cut(s) 1747, 2104, 2221
PspFI CCCAGC 3 cut(s) 2140, 2198, 2207
PspGI CCWGG 3 cut(s) 1747, 2104, 2221
PspN4I GGNNCC 6 cut(s) 178, 834, 1788, 2436, 2802, 2976
PspPI GGNCC 5 cut(s) 833, 1048, 1493, 2219, 2470
PspPPI RGGWCCY 2 cut(s) 833, 1048
PstI CTGCAG 1 cut(s) 2998
PstNI CAGNNNCTG 2 cut(s) 1502, 2222
PsuI RGATCY 6 cut(s) 927, 1069, 1833, 2490, 2800, 2998
PteI GCGCGC 1 cut(s) 2698
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 9 cut(s) 29, 332, 1381, 1514, 1947, 2215, 2363, 2846, 2927
RsaNI GTAC 9 cut(s) 28, 331, 1380, 1513, 1946, 2214, 2362, 2845, 2926
RseI CAYNNNNRTG 3 cut(s) 806, 1617, 2507
SacI GAGCTC 4 cut(s) 257, 614, 749, 861
SapI GCTCTTC 6 cut(s) 262, 602, 837, 1217, 1742, 1986
SaqAI TTAA 2 cut(s) 933, 1260
SatI GCNGC 8 cut(s) 80, 815, 963, 966, 1016, 1401, 1812, 2994
Sau96I GGNCC 5 cut(s) 833, 1048, 1493, 2219, 2470
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 5 cut(s) 1749, 2025, 2106, 2223, 2558
SduI GDGCHC 8 cut(s) 257, 614, 749, 861, 894, 1981, 2377, 2866
SfcI CTRYAG 1 cut(s) 2994
SfoI GGCGCC 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 380
SgrAI CRCCGGYG 1 cut(s) 919
SinI GGWCC 4 cut(s) 833, 1048, 1493, 2470
SmiMI CAYNNNNRTG 3 cut(s) 806, 1617, 2507
SmlI CTYRAG 1 cut(s) 2692
SmoI CTYRAG 1 cut(s) 2692
SpeI ACTAGT 1 cut(s) 674
SphI GCATGC 1 cut(s) 1735
Sse9I AATT 9 cut(s) 149, 170, 334, 471, 2076, 2283, 2740, 2763, 2969
SseBI AGGCCT 1 cut(s) 2539
SsiI CCGC 4 cut(s) 1654, 1811, 2021, 2260
SspDI GGCGCC 1 cut(s) 176
SspI AATATT 1 cut(s) 2088
SstI GAGCTC 4 cut(s) 257, 614, 749, 861
StuI AGGCCT 1 cut(s) 2539
StyD4I CCNGG 5 cut(s) 1747, 2023, 2104, 2221, 2556
StyI CCWWGG 4 cut(s) 731, 2540, 2657, 2978
TaaI ACNGT 7 cut(s) 774, 1335, 1504, 1825, 2237, 2320, 2361
TaiI ACGT 2 cut(s) 144, 3037
TasI AATT 9 cut(s) 149, 170, 334, 471, 2076, 2283, 2740, 2763, 2969
TatI WGTACW 6 cut(s) 330, 1379, 1512, 2361, 2844, 2925
TauI GCSGC 1 cut(s) 1814
TfiI GAWTC 8 cut(s) 937, 1034, 1054, 1207, 1276, 1909, 2150, 2512
Tru1I TTAA 2 cut(s) 933, 1260
Tru9I TTAA 2 cut(s) 933, 1260
TseFI GTSAC 3 cut(s) 948, 1856, 2938
TseI GCWGC 7 cut(s) 79, 814, 962, 965, 1015, 1400, 2993
Tsp45I GTSAC 3 cut(s) 948, 1856, 2938
TspGWI ACGGA 4 cut(s) 142, 191, 1050, 1631
Van91I CCANNNNNTGG 1 cut(s) 3075
VpaK11BI GGWCC 4 cut(s) 833, 1048, 1493, 2470
XapI RAATTY 2 cut(s) 149, 170
XceI RCATGY 4 cut(s) 1144, 1735, 1905, 2932
XcmI CCANNNNNNNNNTGG 1 cut(s) 2962
XmaJI CCTAGG 1 cut(s) 2540
XmiI GTMKAC 3 cut(s) 1236, 2315, 2622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.