Rw4G008530

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000671
Physical Location & Seq
Reverse (-)
18771538 .. 18774969
3432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G008530.1

Sequence Viewer

Length: 1914 bp
ATGGCCGTGTCTGAGCAAGACAGTGTTTTCGACAAGCTTGCATACCTTTCTCTGGTCTCCAGGATTTGCATAGAGCTTGAAAATCATGATGGGGGTCTTGGAGACAAAGTCTTGGCAAAGTTGATAGCCGACATGGGACGAAACTGTGAGGCAGTGGACGAGTTCCATAAAGAGTTGAAGCAAAACGGTATTGACATGCCTGAAAATGTCATGTCTATGTTGTTAACAATTATTCACTCTGTTCTTCCTCCTACAAAGGTTTCTGAGAAGAACAAAATCCCAGACATGGCAATTAACAATAGCATGCCAAGGCATCGAGATGATAAAAGAGGTCAAAGTGAGCTTAGGCGTAGGAGGAGGCATAGAAATTCACAGCAGAATAGGAACTTGCCAATATACAAGTTGAAGAATGAGCTTATTCAAGCTGTGCATGAAAATCAGGTTCTTGTTGTCATCGGTGAGACTGGTTCGGGCAAGACAACTCAGGTAACACAGTATCTTGCTGAAGCAGGTTACACCACAAAGGGCAAGATTGGATGTACTCAACCCCGTAGAGTTGCGGTGACGTCTGTGGCCAAGAGGGTTGCTGAAGAATTTGGTTGTGGATTAGGGGAGGAAGTGGGGTATTCTATTCGTTTTGAAGATTGTACTGGACCAGAAACTGTGATCAAGTACATGACTGATGGGATGCTCTTTAGGGAGATTTTGGTTGATAATGATCTTTCTCAGTACTCTGTGATCATGCTTGATGAAGCTCATGAAAGAACAATCTACACTGATGTCCTCTTTGGATTGTTGAAGCAACTTGTGAAACGACGTCCTGATCTTCGCTTGATTGTCACCTCTGCCACATTGGATGCTGAGAAGTTTTCGGCTTATTTCTTCGACTCTTTATTAACTGTTGTGCAGATTCACTTGACAGAACCTGAGGGCGACATACTTCTCTTCTTGACTGGCGAGGAGGAGATTGAATCTGCTTGTCAGTCTCTGAATGAGAGGATGAAAGAATATGGAAAAGGCGTTCCAGAATTGATCATTCTACCAGCTTATGGTGCCCAACCTAGTGAAGAACAGGCGAAGATATTTAAGCCTACTCCTCCAGGGAAGAGGAAGGTGGTGGTGGCTACCAACATTGCTGAAGCTTCTTTAACCATTGATGGGATATTTTATGTCATTGATTCAGGGTATGCAAAGCAAAATGTGTATAATCCGAAGAAAGGGATTGATCTGCTGACTGTAACTCCAATTTCACAAGCATCAGCCAAGCAACGAGCTGGACGTGCTGGGCGGACAGGACCAGGCAACTGTTACCGCCTTTACACTGAGGCTGCATTTAAGAATGAGATGTCGCCTACTACAGTTCCAGAAATCCAGAGAACAAATCTTGTATATACCACGCTTATGATGAAAGCAATGGGGATAAACGAGGTTCTATCTTTTGACTTCATGGATCCACCGCCTTCCCAAGCGCTTATTTCTGCTTCTAAACAGCTGCAAAATCTAGGAGCATTGGATGCAGAGGGGTCGGTGACAAAATTGGGTAGTAGAATGGCAGTGTTACCTCTAGATCCACCTTTGTCGAAAATGCTGCTTGCCAGTGTGAACCTCGGATGCAGTGAGGAGATTTTGACCATCATTGCAATGGTTGAGGCTGGCAATATCTTCTACAGGCCTAGAGGAAAACAAGCTGAAGCTGATCAGAAAAGGGCCAGATCTGAAGTTGTAAATAGTACTCTTGTTGGCAAAGCATGTAGATATTGTTGTCAAAACTTTGTATTCAGAGTACGTTTACTTTACCCTCTGAAAGGTAGGGAAGACAAGGCTATTAGCATCAAGGAGATCAAGGAGTGTGCACGGAACTGTAAGTCATACTTCATAATCTTTTCATGTTTCTACAAAATTCTAGCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

637

Amino Acids

71.02

Weight (kDa)

8.23

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 137 - 286 4.7e-08 DEAD/DEAH box helicase
AAA_22 PF13401 145 - 279 4.2e-06 AAA domain
Helicase_C PF00271 302 - 432 1.2e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 496 - 522 4.3e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 523 - 572 2.4e-11 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 569, 820
Acc36I ACCTGC 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 1052
AccB7I CCANNNNNTGG 1 cut(s) 1049
AciI CCGC 4 cut(s) 560, 1288, 1312, 1457
AclWI GGATC 3 cut(s) 1445, 1458, 1562
AcoI YGGCCR 2 cut(s) 3, 573
AcsI RAATTY 3 cut(s) 367, 593, 1899
AcuI CTGAAG 5 cut(s) 525, 609, 1158, 1710, 1737
AcyI GRCGYC 2 cut(s) 566, 817
AfaI GTAC 6 cut(s) 541, 649, 674, 731, 1732, 1785
AfeI AGCGCT 1 cut(s) 1470
AfiI CCNNNNNNNGG 6 cut(s) 52, 286, 1049, 1158, 1217, 1805
AgsI TTSAA 7 cut(s) 80, 178, 406, 422, 641, 799, 971
AjiI CACGTC 1 cut(s) 1280
AjnI CCWGG 3 cut(s) 59, 1099, 1297
AloI GAACNNNNNNTCC 2 cut(s) 1005, 1037
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 4 cut(s) 61, 96, 455, 990
Alw44I GTGCAC 1 cut(s) 1851
AlwI GGATC 3 cut(s) 1445, 1458, 1562
AlwNI CAGNNNCTG 3 cut(s) 662, 926, 988
Aor51HI AGCGCT 1 cut(s) 1470
AoxI GGCC 4 cut(s) 3, 573, 1670, 1707
ApaLI GTGCAC 1 cut(s) 1851
ApeKI GCWGC 3 cut(s) 1328, 1492, 1588
ApoI RAATTY 3 cut(s) 367, 593, 1899
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 1 cut(s) 1471
AspS9I GGNCC 3 cut(s) 653, 1295, 1707
AsuHPI GGTGA 4 cut(s) 470, 574, 832, 1540
AvaII GGWCC 2 cut(s) 653, 1295
AxyI CCTNAGG 1 cut(s) 927
BaeGI GKGCMC 2 cut(s) 1057, 1855
BalI TGGCCA 1 cut(s) 575
BamHI GGATCC 1 cut(s) 1450
BanI GGYRCC 1 cut(s) 1052
BbsI GAAGAC 1 cut(s) 1821
Bbv12I GWGCWC 1 cut(s) 1855
BbvI GCAGC 3 cut(s) 1315, 1479, 1575
BccI CCATC 4 cut(s) 83, 677, 1151, 1640
BcgI CGANNNNNNTGC 6 cut(s) 20, 54, 1506, 1540, 1570, 1604
BciT130I CCWGG 3 cut(s) 61, 1101, 1299
BclI TGATCA 4 cut(s) 666, 738, 1032, 1696
BcoDI GTCTC 4 cut(s) 61, 96, 455, 990
BfaI CTAG 5 cut(s) 1062, 1502, 1565, 1674, 1904
BfmI CTRYAG 2 cut(s) 1356, 1666
BfoI RGCGCY 1 cut(s) 1472
BfuAI ACCTGC 1 cut(s) 500
BglII AGATCT 1 cut(s) 1712
BisI GCNGC 3 cut(s) 1329, 1493, 1589
BlsI GCNGC 3 cut(s) 1330, 1494, 1590
BmcAI AGTACT 2 cut(s) 731, 1732
Bme1390I CCNGG 3 cut(s) 61, 1101, 1299
Bme18I GGWCC 2 cut(s) 653, 1295
BmgBI CACGTC 1 cut(s) 1280
BmgT120I GGNCC 3 cut(s) 653, 1295, 1707
BmiI GGNNCC 2 cut(s) 1054, 1452
BmrFI CCNGG 3 cut(s) 61, 1101, 1299
BmsI GCATC 7 cut(s) 322, 678, 847, 1265, 1504, 1601, 1839
BpiI GAAGAC 1 cut(s) 1821
BpmI CTGGAG 2 cut(s) 43, 1083
Bpu10I CCTNAGC 1 cut(s) 344
BsaBI GATNNNNATC 1 cut(s) 717
BsaHI GRCGYC 2 cut(s) 566, 817
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 308, 1100, 1606
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 1225, 1255
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 286, 1049, 1158, 1217, 1805
Bse1I ACTGG 4 cut(s) 469, 655, 958, 1596
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 4 cut(s) 1131, 1419, 1635, 1647
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 3 cut(s) 61, 1101, 1299
BseDI CCNNGG 3 cut(s) 308, 1100, 1606
BseGI GGATG 6 cut(s) 542, 693, 862, 1005, 1519, 1616
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 6 cut(s) 52, 286, 1049, 1158, 1217, 1805
BseMI GCAATG 4 cut(s) 1131, 1419, 1635, 1647
BseMII CTCAG 6 cut(s) 255, 497, 740, 852, 918, 1314
BseNI ACTGG 4 cut(s) 469, 655, 958, 1596
BseRI GAGGAG 5 cut(s) 370, 974, 977, 1086, 1634
BseSI GKGCMC 2 cut(s) 1057, 1855
BseXI GCAGC 3 cut(s) 1315, 1479, 1575
BseYI CCCAGC 1 cut(s) 1283
BsgI GTGCAG 1 cut(s) 926
BshFI GGCC 4 cut(s) 5, 575, 1672, 1709
BshNI GGYRCC 1 cut(s) 1052
BsiHKAI GWGCWC 1 cut(s) 1855
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 6 cut(s) 52, 286, 1049, 1158, 1217, 1805
BsmAI GTCTC 4 cut(s) 61, 96, 455, 990
BsmFI GGGAC 1 cut(s) 150
BsnI GGCC 4 cut(s) 5, 575, 1672, 1709
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 1057, 1855
BspACI CCGC 4 cut(s) 560, 1288, 1312, 1457
BspANI GGCC 4 cut(s) 5, 575, 1672, 1709
BspCNI CTCAG 7 cut(s) 4, 256, 496, 739, 853, 919, 1315
BspHI TCATGA 2 cut(s) 85, 757
BspLI GGNNCC 2 cut(s) 1054, 1452
BspMI ACCTGC 1 cut(s) 500
BspPI GGATC 3 cut(s) 1445, 1458, 1562
BspT107I GGYRCC 1 cut(s) 1052
BspTNI GGTCTC 1 cut(s) 61
BsrDI GCAATG 4 cut(s) 1131, 1419, 1635, 1647
BsrI ACTGG 4 cut(s) 469, 655, 958, 1596
BssECI CCNNGG 3 cut(s) 308, 1100, 1606
BssNI GRCGYC 2 cut(s) 566, 817
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 3 cut(s) 61, 1101, 1299
Bst6I CTCTTC 2 cut(s) 950, 1100
BstACI GRCGYC 2 cut(s) 566, 817
BstAPI GCANNNNNTGC 1 cut(s) 1514
BstC8I GCNNGC 4 cut(s) 39, 305, 1593, 1654
BstDEI CTNAG 8 cut(s) 12, 264, 344, 483, 726, 861, 927, 1323
BstENI CCTNNNNNAGG 1 cut(s) 1803
BstF5I GGATG 6 cut(s) 542, 693, 862, 1005, 1519, 1616
BstH2I RGCGCY 1 cut(s) 1472
BstHHI GCGC 1 cut(s) 1471
BstMAI GTCTC 4 cut(s) 61, 96, 455, 990
BstMWI GCNNNNNNNGC 3 cut(s) 1052, 1280, 1514
BstNI CCWGG 3 cut(s) 61, 1101, 1299
BstNSI RCATGY 3 cut(s) 199, 307, 1752
BstSCI CCNGG 3 cut(s) 59, 1099, 1297
BstSFI CTRYAG 2 cut(s) 1356, 1666
BstSLI GKGCMC 2 cut(s) 1057, 1855
BstV1I GCAGC 3 cut(s) 1315, 1479, 1575
BstV2I GAAGAC 1 cut(s) 1821
BstX2I RGATCY 3 cut(s) 1450, 1567, 1712
BstYI RGATCY 3 cut(s) 1450, 1567, 1712
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 4 cut(s) 5, 575, 1672, 1709
BtrI CACGTC 1 cut(s) 1280
BtsCI GGATG 6 cut(s) 542, 693, 862, 1005, 1519, 1616
BtsI GCAGTG 3 cut(s) 159, 1560, 1621
BtsIMutI CAGTG 7 cut(s) 28, 159, 774, 1320, 1560, 1603, 1621
BveI ACCTGC 1 cut(s) 500
Cac8I GCNNGC 4 cut(s) 39, 305, 1593, 1654
CaiI CAGNNNCTG 3 cut(s) 662, 926, 988
CciI TCATGA 2 cut(s) 85, 757
CfoI GCGC 1 cut(s) 1471
Cfr13I GGNCC 3 cut(s) 653, 1295, 1707
Csp6I GTAC 6 cut(s) 540, 648, 673, 730, 1731, 1784
CviQI GTAC 6 cut(s) 540, 648, 673, 730, 1731, 1784
DdeI CTNAG 8 cut(s) 12, 264, 344, 483, 726, 861, 927, 1323
EaeI YGGCCR 2 cut(s) 3, 573
Eam1104I CTCTTC 2 cut(s) 950, 1100
EarI CTCTTC 2 cut(s) 950, 1100
EciI GGCGGA 1 cut(s) 1303
Eco130I CCWWGG 1 cut(s) 308
Eco147I AGGCCT 1 cut(s) 1672
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 653, 1295
Eco47III AGCGCT 1 cut(s) 1470
Eco57I CTGAAG 5 cut(s) 525, 609, 1158, 1710, 1737
Eco81I CCTNAGG 1 cut(s) 927
EcoNI CCTNNNNNAGG 1 cut(s) 1803
EcoRII CCWGG 3 cut(s) 59, 1099, 1297
EcoT14I CCWWGG 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 308
FalI AAGNNNNNCTT 2 cut(s) 1856, 1888
FaqI GGGAC 1 cut(s) 150
FbaI TGATCA 4 cut(s) 666, 738, 1032, 1696
Fnu4HI GCNGC 3 cut(s) 1329, 1493, 1589
FokI GGATG 6 cut(s) 549, 700, 869, 1012, 1526, 1623
Fsp4HI GCNGC 3 cut(s) 1329, 1493, 1589
FspBI CTAG 5 cut(s) 1062, 1502, 1565, 1674, 1904
GlaI GCGC 1 cut(s) 1470
GluI GCNGC 3 cut(s) 1329, 1493, 1589
GsaI CCCAGC 1 cut(s) 1287
GsuI CTGGAG 2 cut(s) 43, 1083
HaeII RGCGCY 1 cut(s) 1472
HaeIII GGCC 4 cut(s) 5, 575, 1672, 1709
HhaI GCGC 1 cut(s) 1471
Hin1I GRCGYC 2 cut(s) 566, 817
Hin6I GCGC 1 cut(s) 1469
HinP1I GCGC 1 cut(s) 1469
HincII GTYRAC 1 cut(s) 225
HindII GTYRAC 1 cut(s) 225
HindIII AAGCTT 2 cut(s) 35, 1140
HinfI GANTC 4 cut(s) 887, 910, 971, 1178
HpaI GTTAAC 1 cut(s) 225
HphI GGTGA 4 cut(s) 470, 574, 832, 1540
Hpy166II GTNNAC 5 cut(s) 157, 225, 1603, 1790, 1853
Hpy188I TCNGA 9 cut(s) 13, 265, 990, 1212, 1610, 1701, 1717, 1781, 1803
Hpy188III TCNNGA 9 cut(s) 86, 317, 758, 821, 949, 1025, 1364, 1372, 1565
Hpy8I GTNNAC 5 cut(s) 157, 225, 1603, 1790, 1853
Hpy99I CGWCG 1 cut(s) 819
HpyAV CCTTC 2 cut(s) 1105, 1470
HpyCH4IV ACGT 4 cut(s) 566, 817, 1279, 1786
HpyF10VI GCNNNNNNNGC 3 cut(s) 1052, 1280, 1514
HpyF3I CTNAG 8 cut(s) 12, 264, 344, 483, 726, 861, 927, 1323
HpySE526I ACGT 4 cut(s) 566, 817, 1279, 1786
Hsp92I GRCGYC 2 cut(s) 566, 817
HspAI GCGC 1 cut(s) 1469
Ksp22I TGATCA 4 cut(s) 666, 738, 1032, 1696
KspAI GTTAAC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 1505
Lsp1109I GCAGC 3 cut(s) 1315, 1479, 1575
LweI GCATC 7 cut(s) 322, 678, 847, 1265, 1504, 1601, 1839
MaeI CTAG 5 cut(s) 1062, 1502, 1565, 1674, 1904
MaeII ACGT 4 cut(s) 566, 817, 1279, 1786
MaeIII GTNAC 8 cut(s) 487, 512, 562, 838, 1237, 1307, 1528, 1557
MflI RGATCY 3 cut(s) 1450, 1567, 1712
MhlI GDGCHC 2 cut(s) 1057, 1855
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 8 cut(s) 228, 291, 367, 593, 1028, 1245, 1535, 1899
MluNI TGGCCA 1 cut(s) 575
MlyI GAGTC 1 cut(s) 881
Mox20I TGGCCA 1 cut(s) 575
MscI TGGCCA 1 cut(s) 575
MseI TTAA 7 cut(s) 224, 294, 896, 1086, 1148, 1335, 1912
MslI CAYNNNNRTG 4 cut(s) 215, 318, 1400, 1640
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 1492
MspR9I CCNGG 3 cut(s) 61, 1101, 1299
MvaI CCWGG 3 cut(s) 61, 1101, 1299
MwoI GCNNNNNNNGC 3 cut(s) 1052, 1280, 1514
NlaIV GGNNCC 2 cut(s) 1054, 1452
NmuCI GTSAC 3 cut(s) 562, 838, 1528
NspI RCATGY 3 cut(s) 199, 307, 1752
PaeI GCATGC 1 cut(s) 307
PagI TCATGA 2 cut(s) 85, 757
PceI AGGCCT 1 cut(s) 1672
PcsI WCGNNNNNNNCGW 1 cut(s) 1276
PfeI GAWTC 3 cut(s) 910, 971, 1178
PflFI GACNNNGTC 1 cut(s) 107
PflMI CCANNNNNTGG 1 cut(s) 1049
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 3 cut(s) 1330, 1494, 1590
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
Psp6I CCWGG 3 cut(s) 59, 1099, 1297
PspFI CCCAGC 1 cut(s) 1283
PspGI CCWGG 3 cut(s) 59, 1099, 1297
PspN4I GGNNCC 2 cut(s) 1054, 1452
PspPI GGNCC 3 cut(s) 653, 1295, 1707
PstNI CAGNNNCTG 3 cut(s) 662, 926, 988
PsuI RGATCY 3 cut(s) 1450, 1567, 1712
PsyI GACNNNGTC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 1492
RsaI GTAC 6 cut(s) 541, 649, 674, 731, 1732, 1785
RsaNI GTAC 6 cut(s) 540, 648, 673, 730, 1731, 1784
RseI CAYNNNNRTG 4 cut(s) 215, 318, 1400, 1640
SaqAI TTAA 7 cut(s) 224, 294, 896, 1086, 1148, 1335, 1912
SatI GCNGC 3 cut(s) 1329, 1493, 1589
Sau96I GGNCC 3 cut(s) 653, 1295, 1707
ScaI AGTACT 2 cut(s) 731, 1732
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 3 cut(s) 61, 1101, 1299
SduI GDGCHC 2 cut(s) 1057, 1855
SfaNI GCATC 7 cut(s) 322, 678, 847, 1265, 1504, 1601, 1839
SfcI CTRYAG 2 cut(s) 1356, 1666
SinI GGWCC 2 cut(s) 653, 1295
SmiMI CAYNNNNRTG 4 cut(s) 215, 318, 1400, 1640
SphI GCATGC 1 cut(s) 307
Sse9I AATT 8 cut(s) 228, 291, 367, 593, 1028, 1245, 1535, 1899
SseBI AGGCCT 1 cut(s) 1672
SsiI CCGC 4 cut(s) 560, 1288, 1312, 1457
SspMI CTAG 5 cut(s) 1062, 1502, 1565, 1674, 1904
StuI AGGCCT 1 cut(s) 1672
StyD4I CCNGG 3 cut(s) 59, 1099, 1297
StyI CCWWGG 1 cut(s) 308
TaiI ACGT 4 cut(s) 569, 820, 1282, 1789
TaqI TCGA 4 cut(s) 30, 316, 885, 1580
TasI AATT 8 cut(s) 228, 291, 367, 593, 1028, 1245, 1535, 1899
TatI WGTACW 5 cut(s) 539, 647, 672, 729, 1730
TfiI GAWTC 3 cut(s) 910, 971, 1178
Tru1I TTAA 7 cut(s) 224, 294, 896, 1086, 1148, 1335, 1912
Tru9I TTAA 7 cut(s) 224, 294, 896, 1086, 1148, 1335, 1912
TscAI CASTG 7 cut(s) 28, 159, 781, 1327, 1560, 1603, 1621
TseFI GTSAC 3 cut(s) 562, 838, 1528
TseI GCWGC 3 cut(s) 1328, 1492, 1588
Tsp45I GTSAC 3 cut(s) 562, 838, 1528
TspDTI ATGAA 8 cut(s) 447, 765, 774, 1016, 1421, 1435, 1864, 1875
TspGWI ACGGA 1 cut(s) 1870
TspRI CASTG 7 cut(s) 28, 159, 781, 1327, 1560, 1603, 1621
Tth111I GACNNNGTC 1 cut(s) 107
Van91I CCANNNNNTGG 1 cut(s) 1049
VneI GTGCAC 1 cut(s) 1851
VpaK11BI GGWCC 2 cut(s) 653, 1295
XagI CCTNNNNNAGG 1 cut(s) 1803
XapI RAATTY 3 cut(s) 367, 593, 1899
XbaI TCTAGA 1 cut(s) 1564
XceI RCATGY 3 cut(s) 199, 307, 1752
XcmI CCANNNNNNNNNTGG 1 cut(s) 1639
XspI CTAG 5 cut(s) 1062, 1502, 1565, 1674, 1904
ZraI GACGTC 2 cut(s) 567, 818
ZrmI AGTACT 2 cut(s) 731, 1732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.