Rh5DG563200

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000671
Physical Location & Seq
Forward (+)
86761923 .. 86765283
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG563200.1

Sequence Viewer

Length: 3048 bp
ATGGCCGTCAGTGTCAAGAAGCTAGAGTACCTTTCCTTGATTTCGAAAGTCTGTTCGGAACTCGAAACCAATCTAGGGCAGCAGTATGGGGACAAAGCCCTAGCTGAGTTCATAACCGATATGGGACGGAACTCCAAGAACGTGGACGAATTCAATGCCAAGTTGAATGAATTTGGCGCCGAAATCCCTGATTACTTTGTCCGTAATCTTCTCACTATTATTCACGCAGTTCTTGCTCCCAACAAGAACCAGAGCTCTTCTAAAGAAGAAAAAGAAGACAAGCCTAGAGATAGAAGTAGAGAAAGCAGAGGTAAGAATAAGGATCAAGAGTGCAATTCCGGTGAGCCGGAGTTGTATGGAGTTTATAAGGGCAGGGTTTCGAAAGTGATGGATGCAGGTTGCTTTGTTGAGTTGAGTTGTTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCGAAGAGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAGCAAGTTTATGTGAAGGTGGTTTCGATTTCGGGTCAGAAGTTGAGTCTTTCAATGAAGGATGTTGATCAGAAGACAGGGAAGGATTTACTTCCTTTGAAGAAGAGCTCAGATTATGATGCTACTAGGTCGAACCCGTCAATGTCAAAACATGGTTCTGTGAGTAGGACTAGTCTTTCTGGGATTAGGACTGTGGAGGAGGATAGTTTTGTCCAATCTCGTAGGCCAAGGAAGAGAATGAGCTCCCCAGAGAGGTGGGAAATCAAACAGTTGATTGCTTCAGGTGTTTTGAGTGCTAAAGAGTATCCAATGCTTTATCAAGATGAGGAAGCGGATGGCATAATGATGAAGCAGCTCTATAAAGATGAGGGACCTGAGGAAGAGCTTGAAATCGAGCTCAATGAGGAAGCACCGGCCTTTTTGCAAGGGCACAATATGTATTCTGTGGATATGTCGCCGGTGAAGATCTTTAAGAATCCAGAAGGGTCACTGAGCCGTGCAGCTGCACTTCAATCTGCACTGATCAAGGAGCGCCGAGAAATGAGAGATCAGCAGCAAAGAGCAGTGCTGGATTCTATCCCGAAGGACCTGAATCGTCCGTGGGAAGATCCAATGGCACAGAATGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCAGCTTATGACATGCCTGAGTGGAAGAAGGATACTTATGCGAAAGCTATGAGTTTCGGGAAGAGGTCAAGCCTCTCGATTCAGGAGCAAAGGAAGAGCTTGCCTGTCTACAAGTTGAGGGAAGAACTGATTGAGGCAGTGAATGAGAATCAAGTGCTTATTGTCATTGGTGAGACTGGTTCTGGTAAGACAACTCAGCTAACACAGTATCTTGCAGAAGCAGGTTACACGACAAAGGGTAGGATTGGATGTACGCAGCCTCGTAGGGTGGCGGCAATGTCAGTGGCGAAAAGGGTTGCTGAAGAGGTTGGTTGTCGGTTGGGGGAGGAAGTCGGTTATGCCATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACAGATGGTATGCTTCTTAGGGAGATTTTGATGGATGAGAACCTTTCGCAATACTCTGTGATAATGCTCGATGAAGCTCATGAGAGGACAATCCACACGGATGTTCTTTTTGGATTACTGAAGCAACCTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTTACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCAACTGCATGCTCTTCTCTATCCCTGGGAGAACTTTTCCATATAAGGTAAACTATGTCAAGGAGCCAGAAAGTGATTACCTTGATGCGGCTCTAATCACTGTTTTACAGATCCACTTGGAAGAACCTGAGGGTGACATCCTTCTCTTCTTGACCGGACAGGAAGAGATTGATTTTGCATGTCAGATTCTTTATGAGAGGATGAAAGCTCTTGGTAAAAATGTACCCGAGTTGATTATCCTACCAGTTTATAGTGCTCTTCCTAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCGCCGGGGAAGAGGAAAGTAGTTGTGGCTACTAACATTGCCGAGGCATCTCTGACTATTGATGGAATATTTTATGTCGTTGATCCTGGATTTGCAAAACAAAATGTTTATAACCCAAAGCTGGGGCTAGATTCACTGGTCATAACTCCGATTTCACAAGCATCAGCGAAGCAACGAGCTGGGCGTGCTGGGCGTACAGGCCCTGGGAAATGTTACCGTCTCTACACTGAGAGTGCATTCCGCAATGAGATGCCCCCTACTTCAATTCCCGAAATCCAGAGGACAAATCTTGGGTCTACGGTGCTTGCAATGAAAGCAATGGGGATAAATGATCTCCTGTCCTTTGATTTTATGGATCCTCCTGCGCCGCAAGCACTCATTTCCGCAATGGAACAACTGTACAGCTTAGGAGCACTTGATGAAGAGGGTTTCCTAACCAGACTAGGAAGGAAAATGTCAGAGTTTCCTCTGGATCCACCCCTCTCGAAGATGCTCCTCGCCAGTGTGGACCTCGGATGCAGTGATGAGATCCTGACCATCATAGCAATGATTCAGACAGGCAATGTCTTTTACAGGCCTAGGGAGAAACAAGACCGGGCAGATCAGAAGAGAGCAAAGTTTTTCCAGCCAGAAGGAGACCATCTTACTTTACTTGAAGTCTACAAGGCTTGGAAGAGAACAAACTTTTCAGCTCCATGGTGTTTCGACAACTTTCTTCAGTCTCGATCCTTGAGGCGCGCACAGGATGTGAGAAAACAGCTCATGAGCATCATGGATAAGCACAAGCTGGATGTTGTCAGTGCCGGAAACAATTACACAAAGATTAGCAAGGCAATCACGGCCGGCTTTTTCTTCCATGTTGCTAAAAAGGATCCACAGGAAGGTTATAAGACCCTAGTTGAGAGAGCCAGCCAGTGTACATCCATCCAAGCAGTGCTCTCTTCCAGAAGCAACCAGATTGGGTCATCTACAATGAGCTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1015

Amino Acids

114.14

Weight (kDa)

7.77

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 121 - 187 1.4e-10 S1 RNA binding domain
DEAD PF00270 431 - 580 2.5e-07 DEAD/DEAH box helicase
Helicase_C PF00271 623 - 754 7.5e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 816 - 844 6.9e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 845 - 904 6.2e-15 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 366, 2172, 2949
Acc36I ACCTGC 2 cut(s) 386, 1382
AccB1I GGYRCC 1 cut(s) 176
AccI GTMKAC 3 cut(s) 1278, 2357, 2721
AccII CGCG 1 cut(s) 2799
AciI CCGC 8 cut(s) 836, 1442, 1696, 1853, 2063, 2302, 2429, 2445
AcoI YGGCCR 2 cut(s) 3, 2901
AcsI RAATTY 2 cut(s) 149, 170
AcuI CTGAAG 4 cut(s) 768, 1491, 1700, 2762
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 7 cut(s) 29, 1423, 1556, 1989, 2257, 2462, 2980
AfiI CCNNNNNNNGG 7 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942
AgsI TTSAA 9 cut(s) 154, 166, 558, 604, 893, 1016, 1768, 2325, 2717
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 3 cut(s) 1789, 2146, 2263
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
Alw21I GWGCWC 7 cut(s) 257, 614, 749, 903, 2023, 2476, 3000
Alw26I GTCTC 5 cut(s) 1337, 1693, 2285, 2691, 2787
AlwNI CAGNNNCTG 2 cut(s) 1544, 2264
Ama87I CYCGRG 1 cut(s) 1991
AoxI GGCC 6 cut(s) 3, 728, 918, 2260, 2636, 2901
ApeKI GCWGC 6 cut(s) 79, 856, 1004, 1007, 1057, 1426
ApoI RAATTY 2 cut(s) 149, 170
ArsI GACNNNNNNTTYG 4 cut(s) 1692, 1724, 2339, 2371
AspA2I CCTAGG 1 cut(s) 2639
AspLEI GCGC 5 cut(s) 179, 1038, 2428, 2799, 2801
AspS9I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
AsuC2I CCSGG 2 cut(s) 2067, 2657
AsuHPI GGTGA 6 cut(s) 353, 505, 976, 1142, 1352, 1910
AsuII TTCGAA 2 cut(s) 44, 380
AvaI CYCGRG 1 cut(s) 1991
AvaII GGWCC 4 cut(s) 875, 1090, 1535, 2569
AvrII CCTAGG 1 cut(s) 2639
AxyI CCTNAGG 2 cut(s) 879, 1893
BaeGI GKGCMC 1 cut(s) 936
BamHI GGATCC 3 cut(s) 2416, 2533, 2932
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 4 cut(s) 257, 614, 749, 903
BarI GAAGNNNNNNTAC 2 cut(s) 11, 43
BbsI GAAGAC 2 cut(s) 282, 584
Bbv12I GWGCWC 7 cut(s) 257, 614, 749, 903, 2023, 2476, 3000
BbvI GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 1438
BccI CCATC 8 cut(s) 382, 833, 1559, 1585, 2117, 2606, 2709, 2993
BceAI ACGGC 2 cut(s) 984, 2916
BcgI CGANNNNNNTGC 2 cut(s) 137, 171
BciT130I CCWGG 3 cut(s) 1791, 2148, 2265
BciVI GTATCC 2 cut(s) 819, 1195
BclI TGATCA 2 cut(s) 571, 1026
BcnI CCSGG 2 cut(s) 2067, 2657
BcoDI GTCTC 5 cut(s) 1337, 1693, 2285, 2691, 2787
BcuI ACTAGT 1 cut(s) 674
BfoI RGCGCY 2 cut(s) 180, 1039
BfuAI ACCTGC 2 cut(s) 386, 1382
BfuI GTATCC 2 cut(s) 819, 1195
BglII AGATCT 1 cut(s) 969
BisI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1427, 1443, 1854, 2429
BlnI CCTAGG 1 cut(s) 2639
BlsI GCNGC 9 cut(s) 81, 858, 1006, 1009, 1059, 1428, 1444, 1855, 2430
Bme1390I CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
Bme18I GGWCC 4 cut(s) 875, 1090, 1535, 2569
BmeT110I CYCGRG 1 cut(s) 1991
BmgT120I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
BmiI GGNNCC 6 cut(s) 178, 876, 1830, 2418, 2535, 2934
BmrFI CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
BpiI GAAGAC 2 cut(s) 282, 584
Bpu10I CCTNAGC 1 cut(s) 2467
Bpu14I TTCGAA 2 cut(s) 44, 380
BpuEI CTTGAG 1 cut(s) 2812
BpuMI CCSGG 2 cut(s) 2067, 2657
BsaBI GATNNNNATC 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 2 cut(s) 1693, 2691
BsaWI WCCGGW 2 cut(s) 338, 1919
Bsc4I CCNNNNNNNGG 7 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942
Bse118I RCCGGY 3 cut(s) 916, 961, 2903
Bse1I ACTGG 6 cut(s) 1351, 1537, 2009, 2202, 2562, 2974
Bse21I CCTNAGG 2 cut(s) 879, 1893
Bse3DI GCAATG 8 cut(s) 1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 3 cut(s) 1791, 2148, 2265
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 7 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942
BseMI GCAATG 8 cut(s) 1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
BseMII CTCAG 8 cut(s) 96, 627, 870, 986, 1179, 1379, 1884, 2280
BseNI ACTGG 6 cut(s) 1351, 1537, 2009, 2202, 2562, 2974
BsePI GCGCGC 1 cut(s) 2797
BseRI GAGGAG 2 cut(s) 716, 2546
BseSI GKGCMC 1 cut(s) 936
BseX3I CGGCCG 1 cut(s) 2901
BseXI GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 1438
BseYI CCCAGC 3 cut(s) 2182, 2240, 2249
BsgI GTGCAG 3 cut(s) 993, 1005, 1023
Bsh1236I CGCG 1 cut(s) 2799
Bsh1285I CGRYCG 1 cut(s) 2904
BshFI GGCC 6 cut(s) 5, 730, 920, 2262, 2638, 2903
BshNI GGYRCC 1 cut(s) 176
BsiEI CGRYCG 1 cut(s) 2904
BsiHKAI GWGCWC 7 cut(s) 257, 614, 749, 903, 2023, 2476, 3000
BsiHKCI CYCGRG 1 cut(s) 1991
BsiSI CCGG 9 cut(s) 339, 347, 917, 962, 1920, 2066, 2656, 2865, 2904
BslFI GGGAC 3 cut(s) 104, 138, 888
BslI CCNNNNNNNGG 7 cut(s) 75, 756, 1852, 2067, 2182, 2183, 2942
BsmAI GTCTC 5 cut(s) 1337, 1693, 2285, 2691, 2787
BsmBI CGTCTC 1 cut(s) 2285
BsmFI GGGAC 3 cut(s) 104, 138, 888
BsmI GAATGC 1 cut(s) 2297
BsnI GGCC 6 cut(s) 5, 730, 920, 2262, 2638, 2903
Bso31I GGTCTC 2 cut(s) 1693, 2691
BsoBI CYCGRG 1 cut(s) 1991
Bsp119I TTCGAA 2 cut(s) 44, 380
Bsp1286I GDGCHC 8 cut(s) 257, 614, 749, 903, 936, 2023, 2476, 3000
Bsp1407I TGTACA 2 cut(s) 2460, 2978
Bsp19I CCATGG 1 cut(s) 2756
BspACI CCGC 8 cut(s) 836, 1442, 1696, 1853, 2063, 2302, 2429, 2445
BspANI GGCC 6 cut(s) 5, 730, 920, 2262, 2638, 2903
BspCNI CTCAG 8 cut(s) 97, 626, 871, 987, 1180, 1378, 1885, 2281
BspFNI CGCG 1 cut(s) 2799
BspHI TCATGA 2 cut(s) 1639, 2823
BspLI GGNNCC 6 cut(s) 178, 876, 1830, 2418, 2535, 2934
BspMI ACCTGC 2 cut(s) 386, 1382
BspQI GCTCTTC 6 cut(s) 262, 602, 879, 1259, 1784, 2028
BspT104I TTCGAA 2 cut(s) 44, 380
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 2 cut(s) 1693, 2691
BsrDI GCAATG 8 cut(s) 1452, 2097, 2311, 2376, 2385, 2454, 2613, 2629
BsrFI RCCGGY 3 cut(s) 916, 961, 2903
BsrGI TGTACA 2 cut(s) 2460, 2978
BsrI ACTGG 6 cut(s) 1351, 1537, 2009, 2202, 2562, 2974
BssAI RCCGGY 3 cut(s) 916, 961, 2903
BssHII GCGCGC 1 cut(s) 2797
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 3 cut(s) 731, 2639, 2756
Bst2UI CCWGG 3 cut(s) 1791, 2148, 2265
Bst4CI ACNGT 8 cut(s) 697, 774, 1377, 1546, 1867, 2279, 2362, 2460
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 233
BstAUI TGTACA 2 cut(s) 2460, 2978
BstBI TTCGAA 2 cut(s) 44, 380
BstC8I GCNNGC 8 cut(s) 1271, 1775, 2247, 2367, 2433, 2799, 2905, 2971
BstDSI CCRYGG 2 cut(s) 1103, 2756
BstFNI CGCG 1 cut(s) 2799
BstH2I RGCGCY 2 cut(s) 180, 1039
BstHHI GCGC 5 cut(s) 179, 1038, 2428, 2799, 2801
BstMAI GTCTC 5 cut(s) 1337, 1693, 2285, 2691, 2787
BstMCI CGRYCG 1 cut(s) 2904
BstMWI GCNNNNNNNGC 6 cut(s) 233, 2246, 2251, 2375, 2432, 2900
BstNI CCWGG 3 cut(s) 1791, 2148, 2265
BstNSI RCATGY 3 cut(s) 1186, 1777, 1947
BstSCI CCNGG 5 cut(s) 1789, 2065, 2146, 2263, 2655
BstSLI GKGCMC 1 cut(s) 936
BstUI CGCG 1 cut(s) 2799
BstV1I GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 1438
BstV2I GAAGAC 2 cut(s) 282, 584
BstX2I RGATCY 7 cut(s) 969, 1111, 1875, 2416, 2533, 2589, 2932
BstXI CCANNNNNNTGG 2 cut(s) 142, 759
BstYI RGATCY 7 cut(s) 969, 1111, 1875, 2416, 2533, 2589, 2932
BstZI CGGCCG 1 cut(s) 2901
Bsu36I CCTNAGG 2 cut(s) 879, 1893
BsuI GTATCC 2 cut(s) 819, 1195
BsuRI GGCC 6 cut(s) 5, 730, 920, 2262, 2638, 2903
BtgI CCRYGG 2 cut(s) 1103, 2756
BtsI GCAGTG 4 cut(s) 1074, 1314, 2587, 3000
BveI ACCTGC 2 cut(s) 386, 1382
Cac8I GCNNGC 8 cut(s) 1271, 1775, 2247, 2367, 2433, 2799, 2905, 2971
CaiI CAGNNNCTG 2 cut(s) 1544, 2264
CciI TCATGA 2 cut(s) 1639, 2823
CfoI GCGC 5 cut(s) 179, 1038, 2428, 2799, 2801
Cfr10I RCCGGY 3 cut(s) 916, 961, 2903
Cfr13I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
Csp6I GTAC 7 cut(s) 28, 1422, 1555, 1988, 2256, 2461, 2979
CspCI CAANNNNNGTGG 2 cut(s) 1434, 1469
CviQI GTAC 7 cut(s) 28, 1422, 1555, 1988, 2256, 2461, 2979
DinI GGCGCC 1 cut(s) 178
EaeI YGGCCR 2 cut(s) 3, 2901
EagI CGGCCG 1 cut(s) 2901
Ecl136II GAGCTC 4 cut(s) 255, 612, 747, 901
EclXI CGGCCG 1 cut(s) 2901
Eco130I CCWWGG 3 cut(s) 731, 2639, 2756
Eco147I AGGCCT 1 cut(s) 2638
Eco24I GRGCYC 4 cut(s) 257, 614, 749, 903
Eco31I GGTCTC 2 cut(s) 1693, 2691
Eco47I GGWCC 4 cut(s) 875, 1090, 1535, 2569
Eco52I CGGCCG 1 cut(s) 2901
Eco53kI GAGCTC 4 cut(s) 255, 612, 747, 901
Eco57I CTGAAG 4 cut(s) 768, 1491, 1700, 2762
Eco81I CCTNAGG 2 cut(s) 879, 1893
Eco88I CYCGRG 1 cut(s) 1991
EcoICRI GAGCTC 4 cut(s) 255, 612, 747, 901
EcoO109I RGGNCCY 3 cut(s) 875, 1090, 2261
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 1789, 2146, 2263
EcoT14I CCWWGG 3 cut(s) 731, 2639, 2756
EcoT38I GRGCYC 4 cut(s) 257, 614, 749, 903
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 3 cut(s) 731, 2639, 2756
Esp3I CGTCTC 1 cut(s) 2285
FaqI GGGAC 3 cut(s) 104, 138, 888
FauI CCCGC 1 cut(s) 2070
FbaI TGATCA 2 cut(s) 571, 1026
FblI GTMKAC 3 cut(s) 1278, 2357, 2721
Fnu4HI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1427, 1443, 1854, 2429
FriOI GRGCYC 4 cut(s) 257, 614, 749, 903
Fsp4HI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1427, 1443, 1854, 2429
GlaI GCGC 5 cut(s) 178, 1037, 2427, 2798, 2800
GluI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1427, 1443, 1854, 2429
GsaI CCCAGC 3 cut(s) 2186, 2244, 2253
HaeII RGCGCY 2 cut(s) 180, 1039
HaeIII GGCC 6 cut(s) 5, 730, 920, 2262, 2638, 2903
HapII CCGG 9 cut(s) 339, 347, 917, 962, 1920, 2066, 2656, 2865, 2904
HhaI GCGC 5 cut(s) 179, 1038, 2428, 2799, 2801
Hin1I GRCGYC 1 cut(s) 177
Hin6I GCGC 5 cut(s) 177, 1036, 2426, 2797, 2799
HinP1I GCGC 5 cut(s) 177, 1036, 2426, 2797, 2799
HinfI GANTC 9 cut(s) 550, 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
HpaII CCGG 9 cut(s) 339, 347, 917, 962, 1920, 2066, 2656, 2865, 2904
HphI GGTGA 6 cut(s) 353, 505, 976, 1142, 1352, 1910
Hpy166II GTNNAC 7 cut(s) 145, 1279, 1816, 2358, 2569, 2722, 2979
Hpy8I GTNNAC 7 cut(s) 145, 1279, 1816, 2358, 2569, 2722, 2979
HpyCH4III ACNGT 8 cut(s) 697, 774, 1377, 1546, 1867, 2279, 2362, 2460
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 6 cut(s) 233, 2246, 2251, 2375, 2432, 2900
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 177
HspAI GCGC 5 cut(s) 177, 1036, 2426, 2797, 2799
KasI GGCGCC 1 cut(s) 176
KroI GCCGGC 1 cut(s) 2903
KroNI GCCGGC 1 cut(s) 2905
Ksp22I TGATCA 2 cut(s) 571, 1026
LguI GCTCTTC 6 cut(s) 262, 602, 879, 1259, 1784, 2028
LmnI GCTCC 8 cut(s) 241, 752, 1033, 1255, 1828, 2471, 2559, 2758
Lsp1109I GCAGC 6 cut(s) 91, 868, 994, 1016, 1069, 1438
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 5 cut(s) 990, 1394, 1720, 1898, 2273
MflI RGATCY 7 cut(s) 969, 1111, 1875, 2416, 2533, 2589, 2932
MhlI GDGCHC 8 cut(s) 257, 614, 749, 903, 936, 2023, 2476, 3000
MluCI AATT 6 cut(s) 149, 170, 334, 471, 2325, 2872
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 1 cut(s) 559
MroNI GCCGGC 1 cut(s) 2903
MseI TTAA 1 cut(s) 975
MslI CAYNNNNRTG 3 cut(s) 848, 1659, 2606
MspA1I CMGCKG 1 cut(s) 1007
MspI CCGG 9 cut(s) 339, 347, 917, 962, 1920, 2066, 2656, 2865, 2904
MspR9I CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
Mva1269I GAATGC 1 cut(s) 2297
MvaI CCWGG 3 cut(s) 1791, 2148, 2265
MvnI CGCG 1 cut(s) 2799
MwoI GCNNNNNNNGC 6 cut(s) 233, 2246, 2251, 2375, 2432, 2900
NaeI GCCGGC 1 cut(s) 2905
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 2067, 2657
NcoI CCATGG 1 cut(s) 2756
NgoMIV GCCGGC 1 cut(s) 2903
NlaIV GGNNCC 6 cut(s) 178, 876, 1830, 2418, 2535, 2934
NmeAIII GCCGAG 2 cut(s) 1064, 2128
NmuCI GTSAC 2 cut(s) 990, 1898
NspI RCATGY 3 cut(s) 1186, 1777, 1947
NspV TTCGAA 2 cut(s) 44, 380
PaeI GCATGC 1 cut(s) 1777
PagI TCATGA 2 cut(s) 1639, 2823
PasI CCCWGGG 2 cut(s) 1790, 2264
PauI GCGCGC 1 cut(s) 2797
PceI AGGCCT 1 cut(s) 2638
PciSI GCTCTTC 6 cut(s) 262, 602, 879, 1259, 1784, 2028
PcsI WCGNNNNNNNCGW 2 cut(s) 458, 2242
PctI GAATGC 1 cut(s) 2297
PdiI GCCGGC 1 cut(s) 2905
PfeI GAWTC 8 cut(s) 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
PfoI TCCNGGA 1 cut(s) 2146
PkrI GCNGC 9 cut(s) 81, 858, 1006, 1009, 1059, 1428, 1444, 1855, 2430
PleI GAGTC 1 cut(s) 558
PluTI GGCGCC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 558
PpuMI RGGWCCY 2 cut(s) 875, 1090
PsiI TTATAA 3 cut(s) 366, 2172, 2949
Psp124BI GAGCTC 4 cut(s) 257, 614, 749, 903
Psp5II RGGWCCY 2 cut(s) 875, 1090
Psp6I CCWGG 3 cut(s) 1789, 2146, 2263
PspFI CCCAGC 3 cut(s) 2182, 2240, 2249
PspGI CCWGG 3 cut(s) 1789, 2146, 2263
PspN4I GGNNCC 6 cut(s) 178, 876, 1830, 2418, 2535, 2934
PspPI GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
PspPPI RGGWCCY 2 cut(s) 875, 1090
PstNI CAGNNNCTG 2 cut(s) 1544, 2264
PsuI RGATCY 7 cut(s) 969, 1111, 1875, 2416, 2533, 2589, 2932
PteI GCGCGC 1 cut(s) 2797
PvuII CAGCTG 1 cut(s) 1007
RsaI GTAC 7 cut(s) 29, 1423, 1556, 1989, 2257, 2462, 2980
RsaNI GTAC 7 cut(s) 28, 1422, 1555, 1988, 2256, 2461, 2979
RseI CAYNNNNRTG 3 cut(s) 848, 1659, 2606
SacI GAGCTC 4 cut(s) 257, 614, 749, 903
SapI GCTCTTC 6 cut(s) 262, 602, 879, 1259, 1784, 2028
SaqAI TTAA 1 cut(s) 975
SatI GCNGC 9 cut(s) 80, 857, 1005, 1008, 1058, 1427, 1443, 1854, 2429
Sau96I GGNCC 5 cut(s) 875, 1090, 1535, 2261, 2569
SchI GAGTC 1 cut(s) 559
ScrFI CCNGG 5 cut(s) 1791, 2067, 2148, 2265, 2657
SduI GDGCHC 8 cut(s) 257, 614, 749, 903, 936, 2023, 2476, 3000
SfoI GGCGCC 1 cut(s) 178
SfuI TTCGAA 2 cut(s) 44, 380
SgrAI CRCCGGYG 1 cut(s) 961
SinI GGWCC 4 cut(s) 875, 1090, 1535, 2569
SmiMI CAYNNNNRTG 3 cut(s) 848, 1659, 2606
SmlI CTYRAG 1 cut(s) 2791
SmoI CTYRAG 1 cut(s) 2791
SpeI ACTAGT 1 cut(s) 674
SphI GCATGC 1 cut(s) 1777
Sse9I AATT 6 cut(s) 149, 170, 334, 471, 2325, 2872
SseBI AGGCCT 1 cut(s) 2638
SsiI CCGC 8 cut(s) 836, 1442, 1696, 1853, 2063, 2302, 2429, 2445
SspDI GGCGCC 1 cut(s) 176
SspI AATATT 1 cut(s) 2130
SstI GAGCTC 4 cut(s) 257, 614, 749, 903
StuI AGGCCT 1 cut(s) 2638
StyD4I CCNGG 5 cut(s) 1789, 2065, 2146, 2263, 2655
StyI CCWWGG 3 cut(s) 731, 2639, 2756
TaaI ACNGT 8 cut(s) 697, 774, 1377, 1546, 1867, 2279, 2362, 2460
TaiI ACGT 1 cut(s) 144
TasI AATT 6 cut(s) 149, 170, 334, 471, 2325, 2872
TatI WGTACW 3 cut(s) 1554, 2460, 2978
TauI GCSGC 3 cut(s) 1445, 1856, 2431
TfiI GAWTC 8 cut(s) 979, 1076, 1096, 1249, 1318, 1951, 2192, 2611
Tru1I TTAA 1 cut(s) 975
Tru9I TTAA 1 cut(s) 975
TseFI GTSAC 2 cut(s) 990, 1898
TseI GCWGC 6 cut(s) 79, 856, 1004, 1007, 1057, 1426
Tsp45I GTSAC 2 cut(s) 990, 1898
TspDTI ATGAA 9 cut(s) 100, 183, 434, 575, 866, 1647, 1982, 2387, 2496
TspGWI ACGGA 4 cut(s) 142, 191, 1092, 1673
VpaK11BI GGWCC 4 cut(s) 875, 1090, 1535, 2569
XapI RAATTY 2 cut(s) 149, 170
XceI RCATGY 3 cut(s) 1186, 1777, 1947
XmaJI CCTAGG 1 cut(s) 2639
XmiI GTMKAC 3 cut(s) 1278, 2357, 2721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.