Rh4DG092600

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000671
Physical Location & Seq
Reverse (-)
13970649 .. 13978633
7985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG092600.1

Sequence Viewer

Length: 3825 bp
ATGCCCTTATTACCGGACCCGAGTCACCTTCACTCGGATCCCTCCAACCGCAAGCCAGAGCCGTCGCGTCGTCGGCCTCAGCCCACCGTACTCTCATCTTCGGTTGAATTTCTCTCCCCTTTAGGCATGAACTTGAGGTGGTGGGTTATTGTAAAGACGCTTATACCCTTTGTACTCATTTCCTTTATCTGTTTGCTTTCTTTTTGTTGTGTTTCAATATCTCCAAAGATGGCTGCCATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAAGTCTGTTCAGAGCTGGAAACCCATCTAGGGTTTGGGGATAAAGTTCTTGCTGAGTTCATAACTGAGATGGGACGAAACTGTGGGAACGTGGATGAATTTGATGCCAAATTGAAGGAGAACGGTGCCGAGATGCCCGATTACTTTGTCCGCACACTCCTCACTATCATTCATGCAATTCTTCCTCCGAAGCCAAAGTCTGAGAATGACTCAATGATAGAGAGTACTACTGAAGGTAAGAAGACCAAGTTTAAGGCTCTGTCTATTGCTGATAACAGGGATAGGGTAAATGATCTCCAGAGGGAAATTGAGATGGAGACCAAGGAAAAGAAAAATGACCGAGAAGAGTGGGGTGATGAGCGTGAAGAAGATAGGCGTAGAGGTAGAGGTAGAGATAGAGACAGATATGGGGAAAGAGATAGAGACAGATATGGGGAAAGAGATCGAGACAGAGATAGAGATAGGGATAGGAGAGAGAGGGGCAGGGATAGGTATGACAGAGATGAGAGGCGAAAAGATACACATCATGATTATGATAAGGGAAGGCATACAGACCGATCCAATAAGCACATAAGGGATGGGTATCAAGAGGATGAAGATGTTAGAGAAGAGGGTGGTAACAGGAGAGGAGGTGGGGATCAGCGGAGTGGACTGCACCATTCTGATGAGCCTGAATTGTATCAGGTTTATAAGGGTAGGGTATCAAAAGTCATGGACACGGGTTGCTTTGTTCAGTTCAGTGACTTTAGGGGGAAGGAGGGTCTGGTTCATGTTTCACAGATGGCAACGCGGCGTATTGGTAATGCTAAAGATGTGGTGAAGAGGGATCAAGAGGTCTATGTTAAGGTGATTTCAGTTTCTGGTCAGAAGTTGAGCCTTTCAATGAGGGATGTTGATCAGCATACCGGAAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCATAGGACAAACCCTTCGGTATCAAAGCATGATGGGCCTGTGACAAGGACTGGTCTTTCTGGGATTAGAATTGTGGAAGAGAACAATGTTGCCCCTTCTCGGCGGCCATTAAAGAGAATGAGCTCGCCAGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAATATCCAATGTATGATGAGGAAACAGAGGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAGCTTGAAATTGAGCTTAATGAGGATGAGCCAGCCTTTTTGCAAGGGCAAAGCAGGTATTCTGTAGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAGGGGTCATTAAGTCGTGCAGCTGCACTTCAATCTGCACTTATTAAAGAGCGCAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAGCTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCAGTTTTGGGCAGAGGTCAAAGCTCTCACTTCAGGAACAGAGGCAGAGCTTGCCCATCTACAAGCTCAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCGGGCAAGACAACCCAGGTAACACAATATCTTGCAGAAGCAGGTTACACAACAATGGGTAAGATTGGGTGTACACAACCACGTAGGGTGGCCGCGATGTCTGTAGCCAAGAGAGTTGCTGAAGAATTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGCCCTGATACTGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATCGATGAAAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTATCAAGCGGAGACCAGACCTACGTTTGATTGTCACATCTGCTACCTTAGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATACCGGGGAGAACTTTTCCTGTAGAGATACTATACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTCTAATCACTGTTCTACAGATTCACTTAACAGAACCAGAAGGGGACATCCTTCTCTTCTTGACGGGACAGGAAGAAATTGATTTCTCATGCCAGTCACTTTTTGAGAGGATGAAAGGTCTTGGTAAAAATGTACCTGAGTTGATTATCTTACCAGTTTACAGTGCCCTTCCTAGTGAAATGCAGTCTAGGATTTTTGATCCAGCTCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCATCACTAACCATTGATGGGATATTTTATGTAATTGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAAGGGCTGGATTCACTGGTCATAACCCCAATTTCACAAGCATCAGCCAAGCAAAGAGCTGGGCGCGCTGGGCGTACTGGGCCTGGAAAGTGTTACCGCCTCTACACTGAGAGTGCATACCGCAACGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGTTAACTACACTTACCATGAAAGCAATGGGGATAAACGATCTGTTGTCTTTTGATTTTATGGATCCTCCCTCACCCCAAGCACTCATTTCTGCTATGGAACAACTGTACAGTTTAGGAGCACTCGACGAGGAGGGGCTTCTCACCAAACTGGGTAGGAAAATGGCTGAGTTTCCTCTTGATCCACCACTATCTAAGATGCTACTGGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATGATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTGTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCATGGTGCTTTGAGAACTTCGTTCAGTCTCGATCCCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGATGTTGTGAGTGCTGGAAAGAATTTTACACAGATCAGGAAGGCAATTACGGCGGGATTTTTCTTCCATGGTGCTAGAAAGGACCCTCAGGAGGGGTATAGAACCTTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCGCTCTTCCAGAGACAACCAGATTGGATCATCTACCATGAGTTGGTTATGACTACGAAGGAGTATATGCGTGAGGTAACCGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAAGTGGCAGACCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAGCGTGTTGAACCGCTATATGACAGATACCATGAACCAAACTCTTGGCGTCTTAGTAAACGACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1274

Amino Acids

145.53

Weight (kDa)

6.62

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 319 - 389 1.5e-11 S1 RNA binding domain
DEAD PF00270 628 - 777 1.4e-07 DEAD/DEAH box helicase
AAA_22 PF13401 636 - 761 3.2e-06 AAA domain
Helicase_C PF00271 821 - 951 9.6e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1013 - 1041 8.9e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1042 - 1101 3.3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1159 - 1235 9.1e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 972, 2763
Acc36I ACCTGC 2 cut(s) 1521, 1973
AccB1I GGYRCC 1 cut(s) 407
AccB7I CCANNNNNTGG 2 cut(s) 1718, 3628
AccII CGCG 4 cut(s) 67, 1072, 2036, 2838
AcoI YGGCCR 3 cut(s) 1337, 2031, 3150
AcsI RAATTY 5 cut(s) 107, 380, 2066, 2925, 3463
AcuI CTGAAG 4 cut(s) 534, 1826, 2082, 2898
AcyI GRCGYC 1 cut(s) 3802
AjiI CACGTC 1 cut(s) 3818
AjnI CCWGG 6 cut(s) 1956, 2656, 2737, 2854, 3246, 3701
Alw21I GWGCWC 3 cut(s) 1220, 1358, 3067
AlwNI CAGNNNCTG 2 cut(s) 1142, 1718
Ama87I CYCGRG 2 cut(s) 19, 260
ApeKI GCWGC 6 cut(s) 233, 1595, 1598, 1645, 1648, 2419
ApoI RAATTY 5 cut(s) 107, 380, 2066, 2925, 3463
ArsI GACNNNNNNTTYG 4 cut(s) 464, 496, 1231, 1263
Asp700I GAANNNNTTC 1 cut(s) 1890
AspA2I CCTAGG 1 cut(s) 3230
AspLEI GCGC 5 cut(s) 1629, 2838, 2840, 3589, 3744
AspS9I GGNCC 7 cut(s) 16, 1268, 2126, 2852, 3160, 3344, 3523
AsuC2I CCSGG 1 cut(s) 2382
AsuHPI GGTGA 9 cut(s) 17, 647, 1111, 1141, 1542, 1733, 1943, 3009, 3079
AvaI CYCGRG 2 cut(s) 19, 260
AvaII GGWCC 3 cut(s) 16, 3160, 3523
AvrII CCTAGG 1 cut(s) 3230
AxyI CCTNAGG 3 cut(s) 1393, 3383, 3528
BaeGI GKGCMC 2 cut(s) 2614, 3394
BalI TGGCCA 1 cut(s) 3152
BamHI GGATCC 2 cut(s) 37, 3007
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 2 cut(s) 1220, 1358
BarI GAAGNNNNNNTAC 2 cut(s) 248, 280
BbsI GAAGAC 2 cut(s) 530, 1811
Bbv12I GWGCWC 3 cut(s) 1220, 1358, 3067
BbvCI CCTCAGC 3 cut(s) 78, 1470, 2694
BbvI GCAGC 6 cut(s) 220, 1585, 1607, 1657, 1660, 2431
BceAI ACGGC 2 cut(s) 46, 3507
BciT130I CCWGG 6 cut(s) 1958, 2658, 2739, 2856, 3248, 3703
BclI TGATCA 2 cut(s) 1177, 3253
BcnI CCSGG 1 cut(s) 2382
BfaI CTAG 6 cut(s) 311, 2619, 2634, 3231, 3516, 3695
BfmI CTRYAG 4 cut(s) 1539, 2043, 2397, 2462
BfuAI ACCTGC 2 cut(s) 1521, 1973
BglII AGATCT 1 cut(s) 3180
BisI GCNGC 9 cut(s) 234, 1073, 1337, 1596, 1599, 1646, 1649, 2034, 2420
BlnI CCTAGG 1 cut(s) 3230
BlsI GCNGC 9 cut(s) 235, 1074, 1338, 1597, 1600, 1647, 1650, 2035, 2421
BmcAI AGTACT 2 cut(s) 508, 2204
Bme1390I CCNGG 7 cut(s) 1958, 2382, 2658, 2739, 2856, 3248, 3703
Bme18I GGWCC 3 cut(s) 16, 3160, 3523
BmeT110I CYCGRG 2 cut(s) 19, 260
BmgBI CACGTC 1 cut(s) 3818
BmgT120I GGNCC 7 cut(s) 16, 1268, 2126, 2852, 3160, 3344, 3523
BmiI GGNNCC 5 cut(s) 18, 39, 409, 3009, 3525
BmrFI CCNGG 7 cut(s) 1958, 2382, 2658, 2739, 2856, 3248, 3703
BmrI ACTGGG 2 cut(s) 2859, 3104
BmuI ACTGGG 2 cut(s) 2859, 3104
BoxI GACNNNNGTC 1 cut(s) 21
BpiI GAAGAC 2 cut(s) 530, 1811
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 3 cut(s) 78, 1470, 2694
BpuEI CTTGAG 2 cut(s) 154, 1862
BpuMI CCSGG 1 cut(s) 2382
Bsa29I ATCGAT 1 cut(s) 2184
BsaAI YACGTR 1 cut(s) 2024
BsaBI GATNNNNATC 5 cut(s) 804, 864, 1176, 1686, 2180
BsaHI GRCGYC 1 cut(s) 3802
BsaI GGTCTC 3 cut(s) 593, 2284, 3282
BsaWI WCCGGW 2 cut(s) 13, 1187
BsaXI ACNNNNNCTCC 2 cut(s) 898, 928
Bse21I CCTNAGG 3 cut(s) 1393, 3383, 3528
Bse3DI GCAATG 1 cut(s) 2976
Bse8I GATNNNNATC 5 cut(s) 804, 864, 1176, 1686, 2180
BseBI CCWGG 6 cut(s) 1958, 2658, 2739, 2856, 3248, 3703
BseCI ATCGAT 1 cut(s) 2184
BseJI GATNNNNATC 5 cut(s) 804, 864, 1176, 1686, 2180
BseMI GCAATG 1 cut(s) 2976
BsePI GCGCGC 1 cut(s) 2836
BseRI GAGGAG 3 cut(s) 431, 924, 3089
BseSI GKGCMC 2 cut(s) 2614, 3394
BseXI GCAGC 6 cut(s) 220, 1585, 1607, 1657, 1660, 2431
BseYI CCCAGC 2 cut(s) 2831, 2840
BsgI GTGCAG 4 cut(s) 920, 1584, 1596, 1614
Bsh1236I CGCG 4 cut(s) 67, 1072, 2036, 2838
BshNI GGYRCC 1 cut(s) 407
BshVI ATCGAT 1 cut(s) 2184
BsiHKAI GWGCWC 3 cut(s) 1220, 1358, 3067
BsiHKCI CYCGRG 2 cut(s) 19, 260
BsiSI CCGG 3 cut(s) 14, 1188, 2381
BslFI GGGAC 4 cut(s) 369, 2504, 2526, 2890
BsmFI GGGAC 4 cut(s) 369, 2504, 2526, 2890
Bso31I GGTCTC 3 cut(s) 593, 2284, 3282
BsoBI CYCGRG 2 cut(s) 19, 260
Bsp1286I GDGCHC 5 cut(s) 1220, 1358, 2614, 3067, 3394
Bsp1407I TGTACA 2 cut(s) 2012, 3051
Bsp19I CCATGG 2 cut(s) 3347, 3508
BspDI ATCGAT 1 cut(s) 2184
BspFNI CGCG 4 cut(s) 67, 1072, 2036, 2838
BspHI TCATGA 2 cut(s) 808, 2230
BspLI GGNNCC 5 cut(s) 18, 39, 409, 3009, 3525
BspMI ACCTGC 2 cut(s) 1521, 1973
BspQI GCTCTTC 3 cut(s) 1208, 1470, 3596
BspT107I GGYRCC 1 cut(s) 407
BspTNI GGTCTC 3 cut(s) 593, 2284, 3282
BsrDI GCAATG 1 cut(s) 2976
BsrGI TGTACA 2 cut(s) 2012, 3051
BssHII GCGCGC 1 cut(s) 2836
BssNI GRCGYC 1 cut(s) 3802
BssT1I CCWWGG 7 cut(s) 273, 603, 1694, 3163, 3230, 3347, 3508
Bst2UI CCWGG 6 cut(s) 1958, 2658, 2739, 2856, 3248, 3703
BstACI GRCGYC 1 cut(s) 3802
BstAPI GCANNNNNTGC 1 cut(s) 1861
BstAUI TGTACA 2 cut(s) 2012, 3051
BstBAI YACGTR 1 cut(s) 2024
BstC8I GCNNGC 6 cut(s) 53, 1358, 1509, 1862, 2838, 3746
BstDSI CCRYGG 2 cut(s) 3347, 3508
BstEII GGTNACC 1 cut(s) 3661
BstFNI CGCG 4 cut(s) 67, 1072, 2036, 2838
BstHHI GCGC 5 cut(s) 1629, 2838, 2840, 3589, 3744
BstNI CCWGG 6 cut(s) 1958, 2658, 2739, 2856, 3248, 3703
BstNSI RCATGY 1 cut(s) 1777
BstPAI GACNNNNGTC 1 cut(s) 21
BstPI GGTNACC 1 cut(s) 3661
BstSCI CCNGG 7 cut(s) 1956, 2380, 2656, 2737, 2854, 3246, 3701
BstSFI CTRYAG 4 cut(s) 1539, 2043, 2397, 2462
BstSLI GKGCMC 2 cut(s) 2614, 3394
BstUI CGCG 4 cut(s) 67, 1072, 2036, 2838
BstV1I GCAGC 6 cut(s) 220, 1585, 1607, 1657, 1660, 2431
BstV2I GAAGAC 2 cut(s) 530, 1811
BstX2I RGATCY 5 cut(s) 37, 1681, 1702, 3007, 3180
BstXI CCANNNNNNTGG 2 cut(s) 1368, 3798
BstYI RGATCY 5 cut(s) 37, 1681, 1702, 3007, 3180
Bsu15I ATCGAT 1 cut(s) 2184
Bsu36I CCTNAGG 3 cut(s) 1393, 3383, 3528
BsuTUI ATCGAT 1 cut(s) 2184
BtgI CCRYGG 2 cut(s) 3347, 3508
BtgZI GCGATG 1 cut(s) 2051
BtrI CACGTC 1 cut(s) 3818
BtsI GCAGTG 4 cut(s) 1905, 3178, 3315, 3589
BveI ACCTGC 2 cut(s) 1521, 1973
Cac8I GCNNGC 6 cut(s) 53, 1358, 1509, 1862, 2838, 3746
CaiI CAGNNNCTG 2 cut(s) 1142, 1718
CciI TCATGA 2 cut(s) 808, 2230
CfoI GCGC 5 cut(s) 1629, 2838, 2840, 3589, 3744
Cfr13I GGNCC 7 cut(s) 16, 1268, 2126, 2852, 3160, 3344, 3523
ClaI ATCGAT 1 cut(s) 2184
CseI GACGC 4 cut(s) 56, 166, 3737, 3791
EaeI YGGCCR 3 cut(s) 1337, 2031, 3150
Ecl136II GAGCTC 2 cut(s) 1218, 1356
Eco130I CCWWGG 7 cut(s) 273, 603, 1694, 3163, 3230, 3347, 3508
Eco147I AGGCCT 1 cut(s) 3229
Eco24I GRGCYC 2 cut(s) 1220, 1358
Eco31I GGTCTC 3 cut(s) 593, 2284, 3282
Eco47I GGWCC 3 cut(s) 16, 3160, 3523
Eco53kI GAGCTC 2 cut(s) 1218, 1356
Eco57I CTGAAG 4 cut(s) 534, 1826, 2082, 2898
Eco81I CCTNAGG 3 cut(s) 1393, 3383, 3528
Eco88I CYCGRG 2 cut(s) 19, 260
Eco91I GGTNACC 1 cut(s) 3661
EcoICRI GAGCTC 2 cut(s) 1218, 1356
EcoO109I RGGNCCY 1 cut(s) 3523
EcoO65I GGTNACC 1 cut(s) 3661
EcoRII CCWGG 6 cut(s) 1956, 2656, 2737, 2854, 3246, 3701
EcoT14I CCWWGG 7 cut(s) 273, 603, 1694, 3163, 3230, 3347, 3508
EcoT22I ATGCAT 1 cut(s) 2446
EcoT38I GRGCYC 2 cut(s) 1220, 1358
ErhI CCWWGG 7 cut(s) 273, 603, 1694, 3163, 3230, 3347, 3508
FaqI GGGAC 4 cut(s) 369, 2504, 2526, 2890
FauI CCCGC 1 cut(s) 3487
FbaI TGATCA 2 cut(s) 1177, 3253
Fnu4HI GCNGC 9 cut(s) 234, 1073, 1337, 1596, 1599, 1646, 1649, 2034, 2420
FriOI GRGCYC 2 cut(s) 1220, 1358
Fsp4HI GCNGC 9 cut(s) 234, 1073, 1337, 1596, 1599, 1646, 1649, 2034, 2420
FspBI CTAG 6 cut(s) 311, 2619, 2634, 3231, 3516, 3695
GlaI GCGC 5 cut(s) 1628, 2837, 2839, 3588, 3743
GluI GCNGC 9 cut(s) 234, 1073, 1337, 1596, 1599, 1646, 1649, 2034, 2420
GsaI CCCAGC 2 cut(s) 2835, 2844
GsuI CTGGAG 1 cut(s) 563
HapII CCGG 3 cut(s) 14, 1188, 2381
HgaI GACGC 4 cut(s) 56, 166, 3737, 3791
HhaI GCGC 5 cut(s) 1629, 2838, 2840, 3589, 3744
Hin1I GRCGYC 1 cut(s) 3802
Hin6I GCGC 5 cut(s) 1627, 2836, 2838, 3587, 3742
HinP1I GCGC 5 cut(s) 1627, 2836, 2838, 3587, 3742
HincII GTYRAC 2 cut(s) 2949, 3673
HindII GTYRAC 2 cut(s) 2949, 3673
HinfI GANTC 8 cut(s) 22, 491, 1570, 1667, 1687, 2467, 2783, 3202
HpaI GTTAAC 1 cut(s) 2949
HpaII CCGG 3 cut(s) 14, 1188, 2381
HphI GGTGA 9 cut(s) 17, 647, 1111, 1141, 1542, 1733, 1943, 3009, 3079
Hpy166II GTNNAC 8 cut(s) 932, 2013, 2015, 2605, 2949, 3160, 3673, 3812
Hpy8I GTNNAC 8 cut(s) 932, 2013, 2015, 2605, 2949, 3160, 3673, 3812
Hpy99I CGWCG 6 cut(s) 67, 72, 75, 3074, 3671, 3819
HpyCH4IV ACGT 4 cut(s) 372, 2023, 2302, 3817
HpySE526I ACGT 4 cut(s) 372, 2023, 2302, 3817
Hsp92I GRCGYC 1 cut(s) 3802
HspAI GCGC 5 cut(s) 1627, 2836, 2838, 3587, 3742
Ksp22I TGATCA 2 cut(s) 1177, 3253
KspAI GTTAAC 1 cut(s) 2949
LguI GCTCTTC 3 cut(s) 1208, 1470, 3596
LmnI GCTCC 4 cut(s) 1466, 1741, 2656, 3062
Lsp1109I GCAGC 6 cut(s) 220, 1585, 1607, 1657, 1660, 2431
MaeI CTAG 6 cut(s) 311, 2619, 2634, 3231, 3516, 3695
MaeII ACGT 4 cut(s) 372, 2023, 2302, 3817
MflI RGATCY 5 cut(s) 37, 1681, 1702, 3007, 3180
MhlI GDGCHC 5 cut(s) 1220, 1358, 2614, 3067, 3394
MlsI TGGCCA 1 cut(s) 3152
MluNI TGGCCA 1 cut(s) 3152
MlyI GAGTC 2 cut(s) 31, 485
MmeI TCCRAC 2 cut(s) 69, 1644
Mox20I TGGCCA 1 cut(s) 3152
Mph1103I ATGCAT 1 cut(s) 2446
MroXI GAANNNNTTC 1 cut(s) 1890
MscI TGGCCA 1 cut(s) 3152
MseI TTAA 9 cut(s) 534, 1125, 1343, 1494, 1566, 1586, 1620, 2474, 2948
MslI CAYNNNNRTG 4 cut(s) 813, 945, 1994, 2250
Msp20I TGGCCA 1 cut(s) 3152
MspA1I CMGCKG 2 cut(s) 925, 1598
MspI CCGG 3 cut(s) 14, 1188, 2381
MspR9I CCNGG 7 cut(s) 1958, 2382, 2658, 2739, 2856, 3248, 3703
MvaI CCWGG 6 cut(s) 1958, 2658, 2739, 2856, 3248, 3703
MvnI CGCG 4 cut(s) 67, 1072, 2036, 2838
NciI CCSGG 1 cut(s) 2382
NcoI CCATGG 2 cut(s) 3347, 3508
NlaIV GGNNCC 5 cut(s) 18, 39, 409, 3009, 3525
NmeAIII GCCGAG 2 cut(s) 436, 1312
NmuCI GTSAC 6 cut(s) 23, 1022, 1273, 1548, 2311, 2541
NsiI ATGCAT 1 cut(s) 2446
NspI RCATGY 1 cut(s) 1777
PaeR7I CTCGAG 1 cut(s) 260
PagI TCATGA 2 cut(s) 808, 2230
PauI GCGCGC 1 cut(s) 2836
PceI AGGCCT 1 cut(s) 3229
PciSI GCTCTTC 3 cut(s) 1208, 1470, 3596
PdmI GAANNNNTTC 1 cut(s) 1890
PfeI GAWTC 6 cut(s) 1570, 1667, 1687, 2467, 2783, 3202
PflMI CCANNNNNTGG 2 cut(s) 1718, 3628
PfoI TCCNGGA 1 cut(s) 2737
PkrI GCNGC 9 cut(s) 235, 1074, 1338, 1597, 1600, 1647, 1650, 2035, 2421
PleI GAGTC 2 cut(s) 30, 485
PpsI GAGTC 2 cut(s) 30, 485
Ppu21I YACGTR 1 cut(s) 2024
PpuMI RGGWCCY 1 cut(s) 3523
PshAI GACNNNNGTC 1 cut(s) 21
PsiI TTATAA 2 cut(s) 972, 2763
Psp124BI GAGCTC 2 cut(s) 1220, 1358
Psp5II RGGWCCY 1 cut(s) 3523
Psp6I CCWGG 6 cut(s) 1956, 2656, 2737, 2854, 3246, 3701
PspEI GGTNACC 1 cut(s) 3661
PspFI CCCAGC 2 cut(s) 2831, 2840
PspGI CCWGG 6 cut(s) 1956, 2656, 2737, 2854, 3246, 3701
PspN4I GGNNCC 5 cut(s) 18, 39, 409, 3009, 3525
PspPI GGNCC 7 cut(s) 16, 1268, 2126, 2852, 3160, 3344, 3523
PspPPI RGGWCCY 1 cut(s) 3523
PspXI VCTCGAGB 1 cut(s) 260
PstNI CAGNNNCTG 2 cut(s) 1142, 1718
PsuI RGATCY 5 cut(s) 37, 1681, 1702, 3007, 3180
PteI GCGCGC 1 cut(s) 2836
PvuII CAGCTG 1 cut(s) 1598
RseI CAYNNNNRTG 4 cut(s) 813, 945, 1994, 2250
SacI GAGCTC 2 cut(s) 1220, 1358
SapI GCTCTTC 3 cut(s) 1208, 1470, 3596
SaqAI TTAA 9 cut(s) 534, 1125, 1343, 1494, 1566, 1586, 1620, 2474, 2948
SatI GCNGC 9 cut(s) 234, 1073, 1337, 1596, 1599, 1646, 1649, 2034, 2420
Sau96I GGNCC 7 cut(s) 16, 1268, 2126, 2852, 3160, 3344, 3523
ScaI AGTACT 2 cut(s) 508, 2204
SchI GAGTC 2 cut(s) 31, 485
ScrFI CCNGG 7 cut(s) 1958, 2382, 2658, 2739, 2856, 3248, 3703
SduI GDGCHC 5 cut(s) 1220, 1358, 2614, 3067, 3394
SfcI CTRYAG 4 cut(s) 1539, 2043, 2397, 2462
Sfr274I CTCGAG 1 cut(s) 260
SinI GGWCC 3 cut(s) 16, 3160, 3523
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 4 cut(s) 813, 945, 1994, 2250
SmlI CTYRAG 3 cut(s) 133, 260, 1877
SmoI CTYRAG 3 cut(s) 133, 260, 1877
SseBI AGGCCT 1 cut(s) 3229
SspI AATATT 1 cut(s) 2689
SspMI CTAG 6 cut(s) 311, 2619, 2634, 3231, 3516, 3695
SstI GAGCTC 2 cut(s) 1220, 1358
StuI AGGCCT 1 cut(s) 3229
StyD4I CCNGG 7 cut(s) 1956, 2380, 2656, 2737, 2854, 3246, 3701
StyI CCWWGG 7 cut(s) 273, 603, 1694, 3163, 3230, 3347, 3508
TaiI ACGT 4 cut(s) 375, 2026, 2305, 3820
TaqI TCGA 6 cut(s) 261, 727, 2184, 2358, 3069, 3376
TaqII GACCGA 2 cut(s) 636, 852
TatI WGTACW 6 cut(s) 172, 506, 2012, 2145, 2202, 3051
TauI GCSGC 3 cut(s) 1075, 1339, 2036
TfiI GAWTC 6 cut(s) 1570, 1667, 1687, 2467, 2783, 3202
Tru1I TTAA 9 cut(s) 534, 1125, 1343, 1494, 1566, 1586, 1620, 2474, 2948
Tru9I TTAA 9 cut(s) 534, 1125, 1343, 1494, 1566, 1586, 1620, 2474, 2948
TseFI GTSAC 6 cut(s) 23, 1022, 1273, 1548, 2311, 2541
TseI GCWGC 6 cut(s) 233, 1595, 1598, 1645, 1648, 2419
Tsp45I GTSAC 6 cut(s) 23, 1022, 1273, 1548, 2311, 2541
Van91I CCANNNNNTGG 2 cut(s) 1718, 3628
VpaK11BI GGWCC 3 cut(s) 16, 3160, 3523
XapI RAATTY 5 cut(s) 107, 380, 2066, 2925, 3463
XceI RCATGY 1 cut(s) 1777
XcmI CCANNNNNNNNNTGG 3 cut(s) 314, 2968, 3685
XhoI CTCGAG 1 cut(s) 260
XmaJI CCTAGG 1 cut(s) 3230
XmnI GAANNNNTTC 1 cut(s) 1890
XspI CTAG 6 cut(s) 311, 2619, 2634, 3231, 3516, 3695
ZrmI AGTACT 2 cut(s) 508, 2204
Zsp2I ATGCAT 1 cut(s) 2446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.