RLG00000028384

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000671
Physical Location & Seq
Reverse (-)
22051265 .. 22056840
5576 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028384

Sequence Viewer

Length: 3597 bp
ATGGCTGCGATTAATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCCGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTCGATGCCAAATTGAAGGAACACGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCATGCAATTCTTCCTCCTAAGGCAAAGTCCGAGAATGACTCAAAGAAAGAGAGCACTGCTGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTCAAGGATATCCAGAGGGAAATTGAGTTGGAGGCCAAGGAAAAGCGAAATGACAGAGAAGAATGGGGAAATGGGCGTGAAGAAGATAGGCCCAGAGGTAGAGATGGAGGTAGAGGTAGGGGTAGGGAAAGAGATACAAATCGAGACAGAGACAGGGAAAGGGACAGGGACAGGGACAGGGATAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAAACATTATCATGATTATGATAAGGGAAGGCATACAGATCGCTCCAATAAGCACGGGAGGGATGACTATGAAAAGGATGGAGATGTTAGAGAAGATGATGATGATGATAGGAGAGGAAGTAGGGATCAGCGGAATGGATTGCACCATTCTGATGGGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCTCAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCACGTTTCGCAGATTGCTACTCGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTGAAGGTGATTTCAATTTCTGGTCAAAAGTTGAGCCTGTCAATGAGGGATGTTGATCAGCATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCATCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTACGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCCGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTCTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACGCAGTATCTTGCAGAAGCTGGGTACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCGATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTCTGCTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACGGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCTGAGAAGTTTTCAGGTTATTTCTTTGACTGTAACATCTTCACTATCCCCGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCGGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTATATGAGAGGATGAAAGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCGAGGATATTTGATCCAGCTCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCACTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAGCAACGAGCTGGGCGTGCTGGCCGTACAGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTACTACATTAACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAAGGAGATCATCTGACGTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCTTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCCTTAGGAGAGCGCAGGACGTCAGAAAACAGCTTCTAAGCATTATGGATAAGTATAAACTGGACGTTGTGAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGCTGCTAGGAAGGACCCACAGGAGGGGTATAGAACCCTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCTCTCTTCCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGACAGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCACGGTGGCTGATCCCACCAAGATGATGGAGATTGAACGAGGTCCTTGGAAATTGCAATTTTATTTACCAAAGATGGCAGATGACTCCAAGCTGTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1199

Amino Acids

136.59

Weight (kDa)

6.08

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 250 - 317 5.9e-11 S1 RNA binding domain
DEAD PF00270 556 - 705 1e-07 DEAD/DEAH box helicase
AAA_22 PF13401 564 - 697 1.6e-06 AAA domain
Helicase_C PF00271 749 - 879 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 941 - 969 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 970 - 1029 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1087 - 1163 1e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 756, 2547
AatII GACGTC 1 cut(s) 3186
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1502, 3412
AciI CCGC 7 cut(s) 571, 709, 1323, 2071, 2653, 2677, 3439
AcoI YGGCCR 5 cut(s) 1121, 2601, 2626, 2892, 2934
AcsI RAATTY 4 cut(s) 152, 2250, 2709, 3247
AcuI CTGAAG 4 cut(s) 306, 1682, 1866, 2682
AcyI GRCGYC 1 cut(s) 3183
AfaI GTAC 7 cut(s) 38, 208, 1772, 1798, 1931, 1988, 2632
AfiI CCNNNNNNNGG 8 cut(s) 46, 1502, 2499, 2557, 2558, 3316, 3317, 3412
AhlI ACTAGT 1 cut(s) 2572
AjnI CCWGG 7 cut(s) 1313, 1740, 2440, 2521, 2638, 3030, 3485
AjuI GAANNNNNNNTTGG 4 cut(s) 812, 844, 1655, 1687
Alw21I GWGCWC 3 cut(s) 281, 1142, 3373
Alw26I GTCTC 9 cut(s) 477, 483, 772, 1076, 1493, 1712, 2068, 3162, 3376
AlwNI CAGNNNCTG 3 cut(s) 1502, 1766, 2639
Ama87I CYCGRG 2 cut(s) 32, 1177
ApeKI GCWGC 8 cut(s) 5, 1379, 1382, 1429, 1432, 1817, 2203, 3296
ApoI RAATTY 4 cut(s) 152, 2250, 2709, 3247
ArsI GACNNNNNNTTYG 4 cut(s) 2067, 2099, 2510, 2542
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 1674
AspA2I CCTAGG 1 cut(s) 3014
AspLEI GCGC 1 cut(s) 3178
AspS9I GGNCC 9 cut(s) 429, 734, 1052, 1910, 2636, 2944, 3128, 3307, 3536
AsuC2I CCSGG 1 cut(s) 2166
AsuHPI GGTGA 9 cut(s) 895, 925, 1134, 1326, 1517, 1727, 2285, 2793, 3457
AvaI CYCGRG 2 cut(s) 32, 1177
AvaII GGWCC 4 cut(s) 1910, 2944, 3307, 3536
AvrII CCTAGG 1 cut(s) 3014
AxyI CCTNAGG 2 cut(s) 243, 3167
BaeGI GKGCMC 1 cut(s) 2398
BalI TGGCCA 1 cut(s) 2936
BamHI GGATCC 2 cut(s) 2791, 2908
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 1142, 2851
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1595, 2858
Bbv12I GWGCWC 3 cut(s) 281, 1142, 3373
BbvCI CCTCAGC 1 cut(s) 1254
BbvI GCAGC 7 cut(s) 1369, 1391, 1441, 1444, 1804, 2215, 3283
BceAI ACGGC 1 cut(s) 2613
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 7 cut(s) 1315, 1742, 2442, 2523, 2640, 3032, 3487
BciVI GTATCC 1 cut(s) 1212
BclI TGATCA 2 cut(s) 961, 3037
BcnI CCSGG 1 cut(s) 2166
BcoDI GTCTC 9 cut(s) 477, 483, 772, 1076, 1493, 1712, 2068, 3162, 3376
BcuI ACTAGT 1 cut(s) 2572
BfaI CTAG 9 cut(s) 83, 104, 2564, 2573, 2852, 3015, 3300, 3332, 3479
BfmI CTRYAG 3 cut(s) 1827, 2181, 3276
BfuI GTATCC 1 cut(s) 1212
BisI GCNGC 8 cut(s) 6, 1380, 1383, 1430, 1433, 1818, 2204, 3297
BlnI CCTAGG 1 cut(s) 3014
BlsI GCNGC 8 cut(s) 7, 1381, 1384, 1431, 1434, 1819, 2205, 3298
BmcAI AGTACT 1 cut(s) 1988
Bme1390I CCNGG 8 cut(s) 1315, 1742, 2166, 2442, 2523, 2640, 3032, 3487
Bme18I GGWCC 4 cut(s) 1910, 2944, 3307, 3536
BmeT110I CYCGRG 2 cut(s) 32, 1177
BmgT120I GGNCC 9 cut(s) 429, 734, 1052, 1910, 2636, 2944, 3128, 3307, 3536
BmiI GGNNCC 6 cut(s) 181, 2265, 2793, 2848, 2910, 3309
BmrFI CCNGG 8 cut(s) 1315, 1742, 2166, 2442, 2523, 2640, 3032, 3487
BmrI ACTGGG 2 cut(s) 981, 2397
BmuI ACTGGG 2 cut(s) 981, 2397
BpiI GAAGAC 2 cut(s) 1595, 2858
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1254
BpuEI CTTGAG 1 cut(s) 1286
BpuMI CCSGG 1 cut(s) 2166
Bsa29I ATCGAT 2 cut(s) 1034, 2496
BsaAI YACGTR 1 cut(s) 1808
BsaBI GATNNNNATC 3 cut(s) 477, 960, 1470
BsaHI GRCGYC 1 cut(s) 3183
BsaI GGTCTC 3 cut(s) 772, 1076, 2068
BsaWI WCCGGW 2 cut(s) 1144, 3388
BsaXI ACNNNNNCTCC 2 cut(s) 682, 712
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 1502, 2499, 2557, 2558, 3316, 3317, 3412
Bse118I RCCGGY 1 cut(s) 1119
Bse21I CCTNAGG 2 cut(s) 243, 3167
Bse3DI GCAATG 3 cut(s) 2686, 2760, 2976
Bse8I GATNNNNATC 3 cut(s) 477, 960, 1470
BseBI CCWGG 7 cut(s) 1315, 1742, 2442, 2523, 2640, 3032, 3487
BseCI ATCGAT 2 cut(s) 1034, 2496
BseJI GATNNNNATC 3 cut(s) 477, 960, 1470
BseLI CCNNNNNNNGG 8 cut(s) 46, 1502, 2499, 2557, 2558, 3316, 3317, 3412
BseMI GCAATG 3 cut(s) 2686, 2760, 2976
BseMII CTCAG 8 cut(s) 111, 1017, 1245, 1554, 1997, 2109, 2358, 2655
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 2398
BseXI GCAGC 7 cut(s) 1369, 1391, 1441, 1444, 1804, 2215, 3283
BseYI CCCAGC 2 cut(s) 1766, 2615
BsgI GTGCAG 3 cut(s) 1368, 1380, 1398
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 2 cut(s) 1034, 2496
BsiHKAI GWGCWC 3 cut(s) 281, 1142, 3373
BsiHKCI CYCGRG 2 cut(s) 32, 1177
BsiSI CCGG 5 cut(s) 1120, 1145, 2166, 2267, 3389
BslFI GGGAC 8 cut(s) 107, 141, 515, 521, 527, 2310, 2674, 2743
BslI CCNNNNNNNGG 8 cut(s) 46, 1502, 2499, 2557, 2558, 3316, 3317, 3412
BsmAI GTCTC 9 cut(s) 477, 483, 772, 1076, 1493, 1712, 2068, 3162, 3376
BsmFI GGGAC 8 cut(s) 107, 141, 515, 521, 527, 2310, 2674, 2743
Bso31I GGTCTC 3 cut(s) 772, 1076, 2068
BsoBI CYCGRG 2 cut(s) 32, 1177
Bsp1286I GDGCHC 5 cut(s) 281, 1142, 2398, 2851, 3373
Bsp1407I TGTACA 1 cut(s) 1796
BspACI CCGC 7 cut(s) 571, 709, 1323, 2071, 2653, 2677, 3439
BspCNI CTCAG 8 cut(s) 112, 1016, 1246, 1555, 1996, 2110, 2359, 2656
BspDI ATCGAT 2 cut(s) 1034, 2496
BspHI TCATGA 2 cut(s) 589, 2014
BspLI GGNNCC 6 cut(s) 181, 2265, 2793, 2848, 2910, 3309
BspMAI CTGCAG 1 cut(s) 3280
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 772, 1076, 2068
BsrDI GCAATG 3 cut(s) 2686, 2760, 2976
BsrFI RCCGGY 1 cut(s) 1119
BsrGI TGTACA 1 cut(s) 1796
BssAI RCCGGY 1 cut(s) 1119
BssNI GRCGYC 1 cut(s) 3183
BssT1I CCWWGG 9 cut(s) 45, 54, 375, 1478, 2109, 2947, 3014, 3131, 3539
Bst2UI CCWGG 7 cut(s) 1315, 1742, 2442, 2523, 2640, 3032, 3487
Bst6I CTCTTC 9 cut(s) 881, 1089, 1124, 1239, 1842, 2441, 2853, 3038, 3380
BstACI GRCGYC 1 cut(s) 3183
BstAPI GCANNNNNTGC 2 cut(s) 1645, 3275
BstAUI TGTACA 1 cut(s) 1796
BstBAI YACGTR 1 cut(s) 1808
BstC8I GCNNGC 9 cut(s) 1121, 1293, 1646, 2320, 2622, 2626, 2938, 3027, 3280
BstHHI GCGC 1 cut(s) 3178
BstMAI GTCTC 9 cut(s) 477, 483, 772, 1076, 1493, 1712, 2068, 3162, 3376
BstMWI GCNNNNNNNGC 5 cut(s) 1429, 1645, 2621, 3110, 3275
BstNI CCWGG 7 cut(s) 1315, 1742, 2442, 2523, 2640, 3032, 3487
BstNSI RCATGY 2 cut(s) 1561, 2322
BstSCI CCNGG 8 cut(s) 1313, 1740, 2164, 2440, 2521, 2638, 3030, 3485
BstSFI CTRYAG 3 cut(s) 1827, 2181, 3276
BstSLI GKGCMC 1 cut(s) 2398
BstV1I GCAGC 7 cut(s) 1369, 1391, 1441, 1444, 1804, 2215, 3283
BstV2I GAAGAC 2 cut(s) 1595, 2858
BstX2I RGATCY 5 cut(s) 1465, 1486, 2791, 2908, 2964
BstXI CCANNNNNNTGG 2 cut(s) 731, 3520
BstYI RGATCY 5 cut(s) 1465, 1486, 2791, 2908, 2964
Bsu15I ATCGAT 2 cut(s) 1034, 2496
Bsu36I CCTNAGG 2 cut(s) 243, 3167
BsuI GTATCC 1 cut(s) 1212
BsuTUI ATCGAT 2 cut(s) 1034, 2496
BtgZI GCGATG 1 cut(s) 1835
BtsI GCAGTG 4 cut(s) 279, 1689, 2962, 3373
BtsIMutI CAGTG 9 cut(s) 279, 1689, 1905, 2238, 2410, 2486, 2661, 2962, 3373
Cac8I GCNNGC 9 cut(s) 1121, 1293, 1646, 2320, 2622, 2626, 2938, 3027, 3280
CaiI CAGNNNCTG 3 cut(s) 1502, 1766, 2639
CciI TCATGA 2 cut(s) 589, 2014
CfoI GCGC 1 cut(s) 3178
Cfr10I RCCGGY 1 cut(s) 1119
Cfr13I GGNCC 9 cut(s) 429, 734, 1052, 1910, 2636, 2944, 3128, 3307, 3536
ClaI ATCGAT 2 cut(s) 1034, 2496
CsiI ACCWGGT 1 cut(s) 1313
Csp6I GTAC 7 cut(s) 37, 207, 1771, 1797, 1930, 1987, 2631
CviQI GTAC 7 cut(s) 37, 207, 1771, 1797, 1930, 1987, 2631
EaeI YGGCCR 5 cut(s) 1121, 2601, 2626, 2892, 2934
Eam1104I CTCTTC 9 cut(s) 881, 1089, 1124, 1239, 1842, 2441, 2853, 3038, 3380
EarI CTCTTC 9 cut(s) 881, 1089, 1124, 1239, 1842, 2441, 2853, 3038, 3380
EciI GGCGGA 1 cut(s) 586
Ecl136II GAGCTC 1 cut(s) 1140
Eco130I CCWWGG 9 cut(s) 45, 54, 375, 1478, 2109, 2947, 3014, 3131, 3539
Eco147I AGGCCT 1 cut(s) 3013
Eco24I GRGCYC 2 cut(s) 1142, 2851
Eco31I GGTCTC 3 cut(s) 772, 1076, 2068
Eco32I GATATC 2 cut(s) 349, 1038
Eco47I GGWCC 4 cut(s) 1910, 2944, 3307, 3536
Eco53kI GAGCTC 1 cut(s) 1140
Eco57I CTGAAG 4 cut(s) 306, 1682, 1866, 2682
Eco81I CCTNAGG 2 cut(s) 243, 3167
Eco88I CYCGRG 2 cut(s) 32, 1177
EcoICRI GAGCTC 1 cut(s) 1140
EcoO109I RGGNCCY 4 cut(s) 2636, 3128, 3307, 3536
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 7 cut(s) 1313, 1740, 2440, 2521, 2638, 3030, 3485
EcoRV GATATC 2 cut(s) 349, 1038
EcoT14I CCWWGG 9 cut(s) 45, 54, 375, 1478, 2109, 2947, 3014, 3131, 3539
EcoT22I ATGCAT 1 cut(s) 2230
EcoT38I GRGCYC 2 cut(s) 1142, 2851
ErhI CCWWGG 9 cut(s) 45, 54, 375, 1478, 2109, 2947, 3014, 3131, 3539
FalI AAGNNNNNCTT 2 cut(s) 2850, 2882
FaqI GGGAC 8 cut(s) 107, 141, 515, 521, 527, 2310, 2674, 2743
FauI CCCGC 1 cut(s) 3432
FbaI TGATCA 2 cut(s) 961, 3037
Fnu4HI GCNGC 8 cut(s) 6, 1380, 1383, 1430, 1433, 1818, 2204, 3297
FriOI GRGCYC 2 cut(s) 1142, 2851
Fsp4HI GCNGC 8 cut(s) 6, 1380, 1383, 1430, 1433, 1818, 2204, 3297
FspBI CTAG 9 cut(s) 83, 104, 2564, 2573, 2852, 3015, 3300, 3332, 3479
GlaI GCGC 1 cut(s) 3177
GluI GCNGC 8 cut(s) 6, 1380, 1383, 1430, 1433, 1818, 2204, 3297
GsaI CCCAGC 2 cut(s) 1770, 2619
HapII CCGG 5 cut(s) 1120, 1145, 2166, 2267, 3389
HhaI GCGC 1 cut(s) 3178
Hin1I GRCGYC 1 cut(s) 3183
Hin6I GCGC 1 cut(s) 3176
HinP1I GCGC 1 cut(s) 3176
HincII GTYRAC 1 cut(s) 3457
HindII GTYRAC 1 cut(s) 3457
HpaII CCGG 5 cut(s) 1120, 1145, 2166, 2267, 3389
HphI GGTGA 9 cut(s) 895, 925, 1134, 1326, 1517, 1727, 2285, 2793, 3457
Hpy166II GTNNAC 7 cut(s) 209, 835, 1773, 1797, 1799, 2944, 3457
Hpy8I GTNNAC 7 cut(s) 209, 835, 1773, 1797, 1799, 2944, 3457
HpyCH4IV ACGT 5 cut(s) 837, 1807, 3083, 3183, 3228
HpyF10VI GCNNNNNNNGC 5 cut(s) 1429, 1645, 2621, 3110, 3275
HpySE526I ACGT 5 cut(s) 837, 1807, 3083, 3183, 3228
Hsp92I GRCGYC 1 cut(s) 3183
HspAI GCGC 1 cut(s) 3176
KroI GCCGGC 1 cut(s) 1119
KroNI GCCGGC 1 cut(s) 1121
Ksp22I TGATCA 2 cut(s) 961, 3037
LmnI GCTCC 6 cut(s) 626, 1250, 1408, 2263, 2440, 2846
Lsp1109I GCAGC 7 cut(s) 1369, 1391, 1441, 1444, 1804, 2215, 3283
MabI ACCWGGT 1 cut(s) 1313
MaeI CTAG 9 cut(s) 83, 104, 2564, 2573, 2852, 3015, 3300, 3332, 3479
MaeII ACGT 5 cut(s) 837, 1807, 3083, 3183, 3228
MflI RGATCY 5 cut(s) 1465, 1486, 2791, 2908, 2964
MhlI GDGCHC 5 cut(s) 281, 1142, 2398, 2851, 3373
MlsI TGGCCA 1 cut(s) 2936
MluNI TGGCCA 1 cut(s) 2936
MlyI GAGTC 3 cut(s) 257, 1381, 3572
MmeI TCCRAC 3 cut(s) 348, 1428, 1858
Mox20I TGGCCA 1 cut(s) 2936
Mph1103I ATGCAT 1 cut(s) 2230
MroNI GCCGGC 1 cut(s) 1119
MroXI GAANNNNTTC 1 cut(s) 1674
MscI TGGCCA 1 cut(s) 2936
MseI TTAA 7 cut(s) 12, 1278, 1350, 1404, 2234, 2258, 2741
MslI CAYNNNNRTG 7 cut(s) 588, 594, 729, 1778, 2034, 2981, 3515
Msp20I TGGCCA 1 cut(s) 2936
MspA1I CMGCKG 2 cut(s) 709, 1382
MspI CCGG 5 cut(s) 1120, 1145, 2166, 2267, 3389
MspR9I CCNGG 8 cut(s) 1315, 1742, 2166, 2442, 2523, 2640, 3032, 3487
MvaI CCWGG 7 cut(s) 1315, 1742, 2442, 2523, 2640, 3032, 3487
MwoI GCNNNNNNNGC 5 cut(s) 1429, 1645, 2621, 3110, 3275
NaeI GCCGGC 1 cut(s) 1121
NciI CCSGG 1 cut(s) 2166
NgoMIV GCCGGC 1 cut(s) 1119
NlaIV GGNNCC 6 cut(s) 181, 2265, 2793, 2848, 2910, 3309
NmeAIII GCCGAG 5 cut(s) 133, 208, 835, 2503, 2920
NmuCI GTSAC 7 cut(s) 1057, 1332, 1594, 2095, 2273, 2325, 3445
NsiI ATGCAT 1 cut(s) 2230
NspI RCATGY 2 cut(s) 1561, 2322
PaeI GCATGC 1 cut(s) 2322
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 589, 2014
PceI AGGCCT 1 cut(s) 3013
PcsI WCGNNNNNNNCGW 1 cut(s) 2617
PdiI GCCGGC 1 cut(s) 1121
PdmI GAANNNNTTC 1 cut(s) 1674
PfeI GAWTC 7 cut(s) 1013, 1354, 1451, 1471, 1960, 2567, 2986
PflMI CCANNNNNTGG 2 cut(s) 1502, 3412
PkrI GCNGC 8 cut(s) 7, 1381, 1384, 1431, 1434, 1819, 2205, 3298
PleI GAGTC 3 cut(s) 257, 1380, 3572
PpsI GAGTC 3 cut(s) 257, 1380, 3572
Ppu21I YACGTR 1 cut(s) 1808
PpuMI RGGWCCY 2 cut(s) 3307, 3536
PshBI ATTAAT 1 cut(s) 12
PsiI TTATAA 2 cut(s) 756, 2547
Psp124BI GAGCTC 1 cut(s) 1142
Psp5II RGGWCCY 2 cut(s) 3307, 3536
Psp6I CCWGG 7 cut(s) 1313, 1740, 2440, 2521, 2638, 3030, 3485
PspFI CCCAGC 2 cut(s) 1766, 2615
PspGI CCWGG 7 cut(s) 1313, 1740, 2440, 2521, 2638, 3030, 3485
PspN4I GGNNCC 6 cut(s) 181, 2265, 2793, 2848, 2910, 3309
PspPI GGNCC 9 cut(s) 429, 734, 1052, 1910, 2636, 2944, 3128, 3307, 3536
PspPPI RGGWCCY 2 cut(s) 3307, 3536
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 3280
PstNI CAGNNNCTG 3 cut(s) 1502, 1766, 2639
PsuI RGATCY 5 cut(s) 1465, 1486, 2791, 2908, 2964
PvuII CAGCTG 1 cut(s) 1382
RsaI GTAC 7 cut(s) 38, 208, 1772, 1798, 1931, 1988, 2632
RsaNI GTAC 7 cut(s) 37, 207, 1771, 1797, 1930, 1987, 2631
RseI CAYNNNNRTG 7 cut(s) 588, 594, 729, 1778, 2034, 2981, 3515
SacI GAGCTC 1 cut(s) 1142
SaqAI TTAA 7 cut(s) 12, 1278, 1350, 1404, 2234, 2258, 2741
SatI GCNGC 8 cut(s) 6, 1380, 1383, 1430, 1433, 1818, 2204, 3297
Sau96I GGNCC 9 cut(s) 429, 734, 1052, 1910, 2636, 2944, 3128, 3307, 3536
ScaI AGTACT 1 cut(s) 1988
SchI GAGTC 3 cut(s) 257, 1381, 3572
ScrFI CCNGG 8 cut(s) 1315, 1742, 2166, 2442, 2523, 2640, 3032, 3487
SduI GDGCHC 5 cut(s) 281, 1142, 2398, 2851, 3373
SexAI ACCWGGT 1 cut(s) 1313
SfcI CTRYAG 3 cut(s) 1827, 2181, 3276
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 4 cut(s) 1910, 2944, 3307, 3536
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 7 cut(s) 588, 594, 729, 1778, 2034, 2981, 3515
SmlI CTYRAG 2 cut(s) 32, 1265
SmoI CTYRAG 2 cut(s) 32, 1265
SpeI ACTAGT 1 cut(s) 2572
SphI GCATGC 1 cut(s) 2322
SseBI AGGCCT 1 cut(s) 3013
SsiI CCGC 7 cut(s) 571, 709, 1323, 2071, 2653, 2677, 3439
SspI AATATT 1 cut(s) 1347
SspMI CTAG 9 cut(s) 83, 104, 2564, 2573, 2852, 3015, 3300, 3332, 3479
SstI GAGCTC 1 cut(s) 1142
StuI AGGCCT 1 cut(s) 3013
StyD4I CCNGG 8 cut(s) 1313, 1740, 2164, 2440, 2521, 2638, 3030, 3485
StyI CCWWGG 9 cut(s) 45, 54, 375, 1478, 2109, 2947, 3014, 3131, 3539
TaiI ACGT 5 cut(s) 840, 1810, 3086, 3186, 3231
TaqI TCGA 7 cut(s) 33, 156, 481, 1034, 2417, 2496, 3160
TatI WGTACW 3 cut(s) 1796, 1929, 1986
TfiI GAWTC 7 cut(s) 1013, 1354, 1451, 1471, 1960, 2567, 2986
Tru1I TTAA 7 cut(s) 12, 1278, 1350, 1404, 2234, 2258, 2741
Tru9I TTAA 7 cut(s) 12, 1278, 1350, 1404, 2234, 2258, 2741
TscAI CASTG 9 cut(s) 286, 1689, 1912, 2245, 2410, 2493, 2668, 2962, 3373
TseFI GTSAC 7 cut(s) 1057, 1332, 1594, 2095, 2273, 2325, 3445
TseI GCWGC 8 cut(s) 5, 1379, 1382, 1429, 1432, 1817, 2203, 3296
Tsp45I GTSAC 7 cut(s) 1057, 1332, 1594, 2095, 2273, 2325, 3445
TspGWI ACGGA 3 cut(s) 194, 2048, 2276
TspRI CASTG 9 cut(s) 286, 1689, 1912, 2245, 2410, 2493, 2668, 2962, 3373
Van91I CCANNNNNTGG 2 cut(s) 1502, 3412
VpaK11BI GGWCC 4 cut(s) 1910, 2944, 3307, 3536
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 4 cut(s) 152, 2250, 2709, 3247
XceI RCATGY 2 cut(s) 1561, 2322
XcmI CCANNNNNNNNNTGG 2 cut(s) 3469, 3517
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 3014
XmnI GAANNNNTTC 1 cut(s) 1674
XspI CTAG 9 cut(s) 83, 104, 2564, 2573, 2852, 3015, 3300, 3332, 3479
ZraI GACGTC 1 cut(s) 3184
ZrmI AGTACT 1 cut(s) 1988
Zsp2I ATGCAT 1 cut(s) 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.