Rh1BG205800

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000671
Physical Location & Seq
Forward (+)
32437147 .. 32444314
7168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG205800.1

Sequence Viewer

Length: 3588 bp
ATGGCTGCGATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCTGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTCGATGCCAAATTGAAGGAACACGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCACGCAATTCTTCCTCCTAAGGCAAAGTCCGAGAACGACTCAAAGAAAGAGAGCACTGCTGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGTTGGAGGCCAAGGAAAAGCGAAATGACAGAGAAGAATGGGGAGATGGGCGTGAAGAAGATAGGCCCAGAGGTAGAGATGGAGGTAGAGGTGGGGGTAGGGAAAGAGATACAAATCGAGACAGGGACAGGGACAGGGACAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAATCATTATCATGATTATGATAAGGGAAGGCATACAGATCGATCCAATAAGCACAGGAGGGATGAGTATGAAAAGGATGGAGATGTTAGAGAAGATGATGTTGATAGGAGAGGAAGTAGGGATCATCAGAATGGATTGCACCATTCTGATGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCTCAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCTACTCGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTTAAGGTGATTTCAATTTCTGGTCAAAAGTTGAGCCTGTCAATGAGGGATGTTGATCAGCATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCGTCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCCGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTCTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACGCAGTATCTTGCAGAAGCTGGATACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCGATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTCTGCTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCCGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATCCCCGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCGGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTATATGAGAGGATGAAAGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCACTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACGCAATGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAGCAACGAGCTGGGCGTGCTGGCCGTACAGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTATTACATTAACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCGGGGCCTTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGACGTTGTGAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGCTGCTAGGAAGGACCCACAGGAGGGGTATAGAACCCTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCTCTCTTCCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGACAGTCGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCACGGTGGCTGATCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAACGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCCTGGCGTCTTAGTAAACGGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1195

Amino Acids

136.36

Weight (kDa)

6.29

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 243 - 310 4.3e-11 S1 RNA binding domain
DEAD PF00270 549 - 698 1e-07 DEAD/DEAH box helicase
AAA_22 PF13401 557 - 690 1.6e-06 AAA domain
Helicase_C PF00271 742 - 872 1.6e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 934 - 962 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 963 - 1022 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1080 - 1156 1e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 735, 2526
AasI GACNNNNNNGTC 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1481, 3391
AciI CCGC 7 cut(s) 553, 1302, 2050, 2632, 2656, 3418, 3530
AcoI YGGCCR 5 cut(s) 1100, 2580, 2605, 2871, 2913
AcsI RAATTY 4 cut(s) 152, 2229, 2688, 3226
AcuI CTGAAG 4 cut(s) 306, 1661, 1845, 2661
AcyI GRCGYC 1 cut(s) 3565
AfaI GTAC 6 cut(s) 38, 208, 1777, 1910, 1967, 2611
AhlI ACTAGT 1 cut(s) 2551
AjnI CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
AjuI GAANNNNNNNTTGG 4 cut(s) 791, 823, 1634, 1666
Alw21I GWGCWC 3 cut(s) 281, 1121, 3352
Alw26I GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
AlwNI CAGNNNCTG 3 cut(s) 1481, 1745, 2618
Ama87I CYCGRG 2 cut(s) 32, 1156
ApeKI GCWGC 8 cut(s) 5, 1358, 1361, 1408, 1411, 1796, 2182, 3275
ApoI RAATTY 4 cut(s) 152, 2229, 2688, 3226
ArsI GACNNNNNNTTYG 4 cut(s) 2046, 2078, 2489, 2521
Asp700I GAANNNNTTC 1 cut(s) 1653
AspA2I CCTAGG 1 cut(s) 2993
AspLEI GCGC 1 cut(s) 3507
AspS9I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
AsuC2I CCSGG 1 cut(s) 2145
AsuHPI GGTGA 9 cut(s) 874, 904, 1113, 1305, 1496, 1706, 2264, 2772, 3436
AvaI CYCGRG 2 cut(s) 32, 1156
AvaII GGWCC 3 cut(s) 1889, 2923, 3286
AvrII CCTAGG 1 cut(s) 2993
AxyI CCTNAGG 2 cut(s) 243, 3146
BaeGI GKGCMC 2 cut(s) 2377, 3157
BalI TGGCCA 1 cut(s) 2915
BamHI GGATCC 2 cut(s) 2770, 2887
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 1121, 2830
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1574, 2837
Bbv12I GWGCWC 3 cut(s) 281, 1121, 3352
BbvCI CCTCAGC 1 cut(s) 1233
BbvI GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
BceAI ACGGC 1 cut(s) 2592
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
BciVI GTATCC 2 cut(s) 1191, 1742
BclI TGATCA 2 cut(s) 940, 3016
BcnI CCSGG 1 cut(s) 2145
BcoDI GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
BcuI ACTAGT 1 cut(s) 2551
BfaI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
BfmI CTRYAG 3 cut(s) 1806, 2160, 3255
BfuI GTATCC 2 cut(s) 1191, 1742
BisI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
BlnI CCTAGG 1 cut(s) 2993
BlsI GCNGC 8 cut(s) 7, 1360, 1363, 1410, 1413, 1798, 2184, 3277
BmcAI AGTACT 1 cut(s) 1967
Bme1390I CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
Bme18I GGWCC 3 cut(s) 1889, 2923, 3286
BmeT110I CYCGRG 2 cut(s) 32, 1156
BmgT120I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
BmiI GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
BmrFI CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
BmrI ACTGGG 2 cut(s) 960, 2376
BmuI ACTGGG 2 cut(s) 960, 2376
BpiI GAAGAC 2 cut(s) 1574, 2837
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1233
BpuEI CTTGAG 1 cut(s) 1265
BpuMI CCSGG 1 cut(s) 2145
Bsa29I ATCGAT 2 cut(s) 601, 2475
BsaAI YACGTR 1 cut(s) 1787
BsaBI GATNNNNATC 3 cut(s) 477, 939, 1449
BsaHI GRCGYC 1 cut(s) 3565
BsaI GGTCTC 3 cut(s) 751, 1055, 2047
BsaWI WCCGGW 2 cut(s) 1123, 3367
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bse118I RCCGGY 1 cut(s) 1098
Bse21I CCTNAGG 2 cut(s) 243, 3146
Bse3DI GCAATG 4 cut(s) 2538, 2665, 2739, 2955
Bse8I GATNNNNATC 3 cut(s) 477, 939, 1449
BseBI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
BseCI ATCGAT 2 cut(s) 601, 2475
BseJI GATNNNNATC 3 cut(s) 477, 939, 1449
BseMI GCAATG 4 cut(s) 2538, 2665, 2739, 2955
BseMII CTCAG 8 cut(s) 99, 111, 996, 1224, 1533, 1976, 2337, 2634
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 2 cut(s) 2377, 3157
BseXI GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
BseYI CCCAGC 1 cut(s) 2594
BsgI GTGCAG 3 cut(s) 1347, 1359, 1377
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 2 cut(s) 601, 2475
BsiHKAI GWGCWC 3 cut(s) 281, 1121, 3352
BsiHKCI CYCGRG 2 cut(s) 32, 1156
BsiSI CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
BslFI GGGAC 8 cut(s) 107, 141, 503, 509, 515, 2289, 2653, 2722
BsmAI GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
BsmFI GGGAC 8 cut(s) 107, 141, 503, 509, 515, 2289, 2653, 2722
Bso31I GGTCTC 3 cut(s) 751, 1055, 2047
BsoBI CYCGRG 2 cut(s) 32, 1156
Bsp1286I GDGCHC 6 cut(s) 281, 1121, 2377, 2830, 3157, 3352
Bsp1407I TGTACA 1 cut(s) 1775
BspACI CCGC 7 cut(s) 553, 1302, 2050, 2632, 2656, 3418, 3530
BspCNI CTCAG 8 cut(s) 100, 112, 995, 1225, 1534, 1975, 2338, 2635
BspDI ATCGAT 2 cut(s) 601, 2475
BspHI TCATGA 2 cut(s) 571, 1993
BspLI GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
BspMAI CTGCAG 1 cut(s) 3259
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 751, 1055, 2047
BsrDI GCAATG 4 cut(s) 2538, 2665, 2739, 2955
BsrFI RCCGGY 1 cut(s) 1098
BsrGI TGTACA 1 cut(s) 1775
BssAI RCCGGY 1 cut(s) 1098
BssNI GRCGYC 1 cut(s) 3565
BssT1I CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
Bst2UI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
Bst6I CTCTTC 9 cut(s) 860, 1068, 1103, 1218, 1821, 2420, 2832, 3017, 3359
BstACI GRCGYC 1 cut(s) 3565
BstAPI GCANNNNNTGC 2 cut(s) 1624, 3254
BstAUI TGTACA 1 cut(s) 1775
BstBAI YACGTR 1 cut(s) 1787
BstHHI GCGC 1 cut(s) 3507
BstMAI GTCTC 8 cut(s) 477, 751, 1055, 1472, 1691, 2047, 3141, 3355
BstMWI GCNNNNNNNGC 5 cut(s) 1408, 1624, 2600, 3089, 3254
BstNI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
BstNSI RCATGY 2 cut(s) 1540, 2301
BstSCI CCNGG 9 cut(s) 1292, 1719, 2143, 2419, 2500, 2617, 3009, 3464, 3560
BstSFI CTRYAG 3 cut(s) 1806, 2160, 3255
BstSLI GKGCMC 2 cut(s) 2377, 3157
BstV1I GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
BstV2I GAAGAC 2 cut(s) 1574, 2837
BstX2I RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
BstXI CCANNNNNNTGG 1 cut(s) 3561
BstYI RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
Bsu15I ATCGAT 2 cut(s) 601, 2475
Bsu36I CCTNAGG 2 cut(s) 243, 3146
BsuI GTATCC 2 cut(s) 1191, 1742
BsuTUI ATCGAT 2 cut(s) 601, 2475
BtgZI GCGATG 1 cut(s) 1814
BtsI GCAGTG 4 cut(s) 279, 1668, 2941, 3352
BtsIMutI CAGTG 9 cut(s) 279, 1668, 1884, 2217, 2389, 2465, 2640, 2941, 3352
CaiI CAGNNNCTG 3 cut(s) 1481, 1745, 2618
CciI TCATGA 2 cut(s) 571, 1993
CfoI GCGC 1 cut(s) 3507
Cfr10I RCCGGY 1 cut(s) 1098
Cfr13I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
ClaI ATCGAT 2 cut(s) 601, 2475
CseI GACGC 2 cut(s) 3500, 3554
CsiI ACCWGGT 1 cut(s) 1292
Csp6I GTAC 6 cut(s) 37, 207, 1776, 1909, 1966, 2610
CviQI GTAC 6 cut(s) 37, 207, 1776, 1909, 1966, 2610
DrdI GACNNNNNNGTC 1 cut(s) 1009
DseDI GACNNNNNNGTC 1 cut(s) 1009
EaeI YGGCCR 5 cut(s) 1100, 2580, 2605, 2871, 2913
Eam1104I CTCTTC 9 cut(s) 860, 1068, 1103, 1218, 1821, 2420, 2832, 3017, 3359
EarI CTCTTC 9 cut(s) 860, 1068, 1103, 1218, 1821, 2420, 2832, 3017, 3359
EciI GGCGGA 1 cut(s) 568
Ecl136II GAGCTC 1 cut(s) 1119
Eco130I CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
Eco147I AGGCCT 1 cut(s) 2992
Eco24I GRGCYC 2 cut(s) 1121, 2830
Eco31I GGTCTC 3 cut(s) 751, 1055, 2047
Eco32I GATATC 2 cut(s) 349, 1017
Eco47I GGWCC 3 cut(s) 1889, 2923, 3286
Eco53kI GAGCTC 1 cut(s) 1119
Eco57I CTGAAG 4 cut(s) 306, 1661, 1845, 2661
Eco81I CCTNAGG 2 cut(s) 243, 3146
Eco88I CYCGRG 2 cut(s) 32, 1156
EcoICRI GAGCTC 1 cut(s) 1119
EcoO109I RGGNCCY 3 cut(s) 2615, 3107, 3286
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
EcoRV GATATC 2 cut(s) 349, 1017
EcoT14I CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
EcoT22I ATGCAT 1 cut(s) 2209
EcoT38I GRGCYC 2 cut(s) 1121, 2830
ErhI CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
FalI AAGNNNNNCTT 2 cut(s) 2829, 2861
FaqI GGGAC 8 cut(s) 107, 141, 503, 509, 515, 2289, 2653, 2722
FauI CCCGC 1 cut(s) 3411
FbaI TGATCA 2 cut(s) 940, 3016
Fnu4HI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
FriOI GRGCYC 2 cut(s) 1121, 2830
Fsp4HI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
FspBI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
GlaI GCGC 1 cut(s) 3506
GluI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
GsaI CCCAGC 1 cut(s) 2598
HapII CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
HgaI GACGC 2 cut(s) 3500, 3554
HhaI GCGC 1 cut(s) 3507
Hin1I GRCGYC 1 cut(s) 3565
Hin6I GCGC 1 cut(s) 3505
HinP1I GCGC 1 cut(s) 3505
HincII GTYRAC 1 cut(s) 3436
HindII GTYRAC 1 cut(s) 3436
HinfI GANTC 9 cut(s) 263, 992, 1333, 1351, 1430, 1450, 1939, 2546, 2965
HpaII CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
HphI GGTGA 9 cut(s) 874, 904, 1113, 1305, 1496, 1706, 2264, 2772, 3436
Hpy166II GTNNAC 6 cut(s) 209, 1776, 1778, 2923, 3436, 3575
Hpy8I GTNNAC 6 cut(s) 209, 1776, 1778, 2923, 3436, 3575
Hpy99I CGWCG 1 cut(s) 1015
HpyCH4IV ACGT 3 cut(s) 1786, 3207, 3520
HpyF10VI GCNNNNNNNGC 5 cut(s) 1408, 1624, 2600, 3089, 3254
HpySE526I ACGT 3 cut(s) 1786, 3207, 3520
Hsp92I GRCGYC 1 cut(s) 3565
HspAI GCGC 1 cut(s) 3505
KroI GCCGGC 1 cut(s) 1098
KroNI GCCGGC 1 cut(s) 1100
Ksp22I TGATCA 2 cut(s) 940, 3016
LmnI GCTCC 4 cut(s) 1229, 1387, 2242, 2825
Lsp1109I GCAGC 7 cut(s) 1348, 1370, 1420, 1423, 1783, 2194, 3262
MabI ACCWGGT 1 cut(s) 1292
MaeI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
MaeII ACGT 3 cut(s) 1786, 3207, 3520
MflI RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
MhlI GDGCHC 6 cut(s) 281, 1121, 2377, 2830, 3157, 3352
MlsI TGGCCA 1 cut(s) 2915
MluNI TGGCCA 1 cut(s) 2915
MlyI GAGTC 2 cut(s) 257, 1360
MmeI TCCRAC 3 cut(s) 348, 1407, 1837
Mox20I TGGCCA 1 cut(s) 2915
Mph1103I ATGCAT 1 cut(s) 2209
MroNI GCCGGC 1 cut(s) 1098
MroXI GAANNNNTTC 1 cut(s) 1653
MscI TGGCCA 1 cut(s) 2915
MseI TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
MslI CAYNNNNRTG 7 cut(s) 570, 576, 690, 708, 1757, 2013, 2960
Msp20I TGGCCA 1 cut(s) 2915
MspA1I CMGCKG 1 cut(s) 1361
MspI CCGG 5 cut(s) 1099, 1124, 2145, 2246, 3368
MspR9I CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
MvaI CCWGG 8 cut(s) 1294, 1721, 2421, 2502, 2619, 3011, 3466, 3562
MwoI GCNNNNNNNGC 5 cut(s) 1408, 1624, 2600, 3089, 3254
NaeI GCCGGC 1 cut(s) 1100
NciI CCSGG 1 cut(s) 2145
NgoMIV GCCGGC 1 cut(s) 1098
NlaIV GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
NmeAIII GCCGAG 5 cut(s) 208, 814, 2122, 2482, 2899
NmuCI GTSAC 7 cut(s) 1036, 1311, 1573, 2074, 2252, 2304, 3424
NsiI ATGCAT 1 cut(s) 2209
NspI RCATGY 2 cut(s) 1540, 2301
PaeI GCATGC 1 cut(s) 2301
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 571, 1993
PceI AGGCCT 1 cut(s) 2992
PcsI WCGNNNNNNNCGW 1 cut(s) 2596
PdiI GCCGGC 1 cut(s) 1100
PdmI GAANNNNTTC 1 cut(s) 1653
PfeI GAWTC 7 cut(s) 992, 1333, 1430, 1450, 1939, 2546, 2965
PflMI CCANNNNNTGG 2 cut(s) 1481, 3391
PkrI GCNGC 8 cut(s) 7, 1360, 1363, 1410, 1413, 1798, 2184, 3277
PleI GAGTC 2 cut(s) 257, 1359
PpsI GAGTC 2 cut(s) 257, 1359
Ppu21I YACGTR 1 cut(s) 1787
PpuMI RGGWCCY 1 cut(s) 3286
PsiI TTATAA 2 cut(s) 735, 2526
Psp124BI GAGCTC 1 cut(s) 1121
Psp5II RGGWCCY 1 cut(s) 3286
Psp6I CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
PspFI CCCAGC 1 cut(s) 2594
PspGI CCWGG 8 cut(s) 1292, 1719, 2419, 2500, 2617, 3009, 3464, 3560
PspN4I GGNNCC 7 cut(s) 181, 2244, 2772, 2827, 2889, 3108, 3288
PspPI GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
PspPPI RGGWCCY 1 cut(s) 3286
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 1 cut(s) 3259
PstNI CAGNNNCTG 3 cut(s) 1481, 1745, 2618
PsuI RGATCY 5 cut(s) 1444, 1465, 2770, 2887, 2943
PvuII CAGCTG 1 cut(s) 1361
RsaI GTAC 6 cut(s) 38, 208, 1777, 1910, 1967, 2611
RsaNI GTAC 6 cut(s) 37, 207, 1776, 1909, 1966, 2610
RseI CAYNNNNRTG 7 cut(s) 570, 576, 690, 708, 1757, 2013, 2960
SacI GAGCTC 1 cut(s) 1121
SaqAI TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
SatI GCNGC 8 cut(s) 6, 1359, 1362, 1409, 1412, 1797, 2183, 3276
Sau96I GGNCC 7 cut(s) 429, 1031, 1889, 2615, 2923, 3107, 3286
ScaI AGTACT 1 cut(s) 1967
SchI GAGTC 2 cut(s) 257, 1360
ScrFI CCNGG 9 cut(s) 1294, 1721, 2145, 2421, 2502, 2619, 3011, 3466, 3562
SduI GDGCHC 6 cut(s) 281, 1121, 2377, 2830, 3157, 3352
SexAI ACCWGGT 1 cut(s) 1292
SfcI CTRYAG 3 cut(s) 1806, 2160, 3255
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 3 cut(s) 1889, 2923, 3286
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 7 cut(s) 570, 576, 690, 708, 1757, 2013, 2960
SmlI CTYRAG 2 cut(s) 32, 1244
SmoI CTYRAG 2 cut(s) 32, 1244
SpeI ACTAGT 1 cut(s) 2551
SphI GCATGC 1 cut(s) 2301
SseBI AGGCCT 1 cut(s) 2992
SsiI CCGC 7 cut(s) 553, 1302, 2050, 2632, 2656, 3418, 3530
SspI AATATT 1 cut(s) 1326
SspMI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
SstI GAGCTC 1 cut(s) 1121
StuI AGGCCT 1 cut(s) 2992
StyD4I CCNGG 9 cut(s) 1292, 1719, 2143, 2419, 2500, 2617, 3009, 3464, 3560
StyI CCWWGG 8 cut(s) 45, 54, 375, 1457, 2088, 2926, 2993, 3110
TaiI ACGT 3 cut(s) 1789, 3210, 3523
TaqI TCGA 9 cut(s) 33, 156, 481, 601, 1013, 2121, 2396, 2475, 3139
TatI WGTACW 3 cut(s) 1775, 1908, 1965
TfiI GAWTC 7 cut(s) 992, 1333, 1430, 1450, 1939, 2546, 2965
Tru1I TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
Tru9I TTAA 7 cut(s) 888, 1257, 1329, 1383, 2213, 2237, 2720
TscAI CASTG 9 cut(s) 286, 1668, 1891, 2224, 2389, 2472, 2647, 2941, 3352
TseFI GTSAC 7 cut(s) 1036, 1311, 1573, 2074, 2252, 2304, 3424
TseI GCWGC 8 cut(s) 5, 1358, 1361, 1408, 1411, 1796, 2182, 3275
Tsp45I GTSAC 7 cut(s) 1036, 1311, 1573, 2074, 2252, 2304, 3424
TspGWI ACGGA 2 cut(s) 194, 2255
TspRI CASTG 9 cut(s) 286, 1668, 1891, 2224, 2389, 2472, 2647, 2941, 3352
Van91I CCANNNNNTGG 2 cut(s) 1481, 3391
VpaK11BI GGWCC 3 cut(s) 1889, 2923, 3286
XapI RAATTY 4 cut(s) 152, 2229, 2688, 3226
XceI RCATGY 2 cut(s) 1540, 2301
XcmI CCANNNNNNNNNTGG 1 cut(s) 3448
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2993
XmnI GAANNNNTTC 1 cut(s) 1653
XspI CTAG 9 cut(s) 83, 104, 2543, 2552, 2831, 2994, 3279, 3311, 3458
ZrmI AGTACT 1 cut(s) 1967
Zsp2I ATGCAT 1 cut(s) 2209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.