Prupe.1G368500_v2.0.a1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000671
Physical Location & Seq
Reverse (-)
33704974 .. 33709003
4030 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G368500.1

Sequence Viewer

Length: 2838 bp
ATGCAGAGTGGAGAGCTTTATCAAGAAGAGGGAGTCAAGGACATCAAGAAGGAAATTGAGTTGGAGACCAAGGAAAAGGAAGGAGATAGAGATAGAGATAGACACAGAGGAGGTATAGATAGAGATGGGAGGGAGAGGCGAAGGGAAAGGTATGATAGAGATGAGAGGCGTAGAGATACACATTATAATGATGATGGTGATGACAGGGGAAGGCATACAGATCGATACAATAAGCATAAGAGAGATGGGTATGAAGACGATGGAGATGTTAAGGAAGATGGTGATGATAGGAGAGGTAATAGGGATCGGCAGAATGGCCAGAGCCATTCGGATGAACCCAAATTGTATCAGGTTTATAAGGGCAGGGTTTCCAGAGTGATGGACACCGGTTGCTTCGTTCAGTTGAATGATTTAAGAGGGAATGAGAGTCTGATGGCAACTCGGCGAATTAGTAATGCTAAGGATGTGGTGAAGAGGGATCAGGAAGTTTATGTGAAGGTGATTTCAATTTCAGGTCAGAAGTTGAGCCTTTCGATTTGGGATGTTGATCAATATACAGGGAAGGATTTGCTACCCTTGAAGAAGAGCTCAGAGGATGATGCTCTCAGGACGAACCCATCTTTTTCAAAGGATGGACCTATGACTAGGACTGGTCTTTCCGGGATTAGGATTGTGGAAGAGGATGAGGTTGGCCCATCACGCCGGCCATTGAAGAGAATGAGTTCGCCAGAGAAGTGGGAAGCCAAACGGTTGATTGCCTCAGAAGAGGGAGCTGAGGACGAGCTTGAGATTGAGCTCACTGAGGACGAGCCAGCCTTTTTAAATGGACAGAGCAGGTATTCTGTTGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAACTGTCACTGGGTCGTGCAGCTGCACTTCAGTCCACACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCTATCCCAAAGGATCTAAATCGTCCCTCGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAAGAATTTAGAGGTGTTGGTTTGTCGGCATATGATATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAAACCATCAGTTTTGGGCAGAAGCCAAAACTCTCAATCCAGGAACAGAGGCAGAGCTTGCCTATCTACAAGCTGAAGAAAGAATTGATTGTGGCGGTGCATGAGAACCAAGTGCTCGTCGTCATTGGTGAGACTGGTTCAGGTAAGACAACACAGGTAACGCAGTATCTTGCAGAAGTGGGTTACACGACAATGGGTAAGATTGGTTGTACACAACCACGTATGGTGGCTGCCATGTCTGTGGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTTTAGGAGAGGAAAACCGTTCTCAATATTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCACACAGATGTTCTTTTTGGACTGCTGAAGAAGCTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACTTTGGATGCTGAGAAGTTCTCTGGTTATTTTTTCAACTGTAACATCTTTACTATCCCTGGGAGAACTTTTCCTGTAGAGATACTCTACACTAAACAGCCCGAAAGTGACTACCTTGATGCATCTCTAATAACTGTTCTACAAATACATTTAACAGAACCTGAAGGTGACATCCTTCTTTTCTTGACTGGACAAGAAGAGATTGATTTTGCATGCCATTCTCTTTATGAGAGGATGAAAGGTCTTGGTAAAAATGTTCCTGAGTTGATTATCCTACCAGTGTATAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATATTTGATCCTGCCCCACCAGGTAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGCATCACTGACCATTGATGGGATATTTTATGTCATTGATCCTGGTTTTGCAAAGCAAAATTTTTATAACCCAAAGCAGGGGCTAGATTCACTGGTCATAACTCCAATGTCCCAAGCATCAGCGAAGCAACGAGCTGGGCATGCTGGGCGTACAGGCCCCGGGAAATGTTACCGCCTCTACACTGAGAGTGCATACTGCAATGAGATGTCCCCTACTTCAATTCCAGAAATCCAGAGGATAAATCTTGGGACTACTACACTTACGATGAAAGCTATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGTATCCTCCTTCACCCCAAGCACTCATTTCTGCCACGGAGCAATTGTACAGTTTAGGAGCACTGGATGAGGAGGGGCTGCTCACCAAATTGGGTATGCAAATGGCTGAGTTTCCTCTGGATCCACCCTTATCTAAGATGCTATTGGCCAGTGCGGACCTTGGATGCAGTGATGAGATCTTGACCATCATTGCAATGATTCAGACAGACATGTTAGTCATTTTGATACTGGAATTGTTTTTCTTGATTGTGAATTTCAGGTATAAACTGGATGTTGTCAGTGCTGGAAAGAATTTTCCAAAGATCAGGAAGGCAATTACAGCAGGAAAGGACCCGCAGGAGGGTTATAGAACCCTAGTGAAGAACCAACCAGTTTATATACACCCAAGCAGCGCCCTCTTCCAGAGACAACCAGATTGGGTGATCTACCATGAACTGGTTATGACTACAAAGGAGTACATGCGTGAGGTGACGGTCGTCGACCCCAAATGGCTCGTGGAACTGGCACCCAGGTTCTTCAAGGTGGCAGATCCCACCAAGATGAGCAAGCACAAGCGTCAAGAACGTATTGAACCGCTATATGATAGGTACCATGAACCTAACTCTTGGCGTCTTAGTAAGCGATGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

946

Amino Acids

107.59

Weight (kDa)

6.25

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 186, 357, 1965
AasI GACNNNNNNGTC 1 cut(s) 2420
Acc36I ACCTGC 1 cut(s) 825
Acc65I GGTACC 1 cut(s) 2793
AccB1I GGYRCC 2 cut(s) 2710, 2793
AccB7I CCANNNNNTGG 2 cut(s) 1022, 2641
AccI GTMKAC 1 cut(s) 2685
AciI CCGC 6 cut(s) 1202, 1492, 2071, 2360, 2540, 2780
AclWI GGATC 9 cut(s) 312, 486, 993, 1001, 1844, 1931, 2321, 2334, 2729
AcoI YGGCCR 4 cut(s) 316, 704, 1350, 2352
AcsI RAATTY 4 cut(s) 1046, 1957, 2458, 2497
AcuI CTGAAG 5 cut(s) 893, 1202, 1386, 1496, 1710
AcyI GRCGYC 1 cut(s) 2815
AfaI GTAC 5 cut(s) 1318, 2050, 2255, 2663, 2795
AfiI CCNNNNNNNGG 8 cut(s) 666, 1022, 1917, 1975, 1976, 2545, 2546, 2641
AflIII ACRYGT 1 cut(s) 2415
AgeI ACCGGT 1 cut(s) 386
AgsI TTSAA 9 cut(s) 406, 507, 580, 627, 712, 1564, 2118, 2725, 2777
AjnI CCWGG 5 cut(s) 1146, 1585, 1861, 1939, 2714
Alw21I GWGCWC 4 cut(s) 590, 798, 1224, 2269
Alw26I GTCTC 5 cut(s) 59, 1013, 1232, 1489, 2605
AlwI GGATC 9 cut(s) 312, 486, 993, 1001, 1844, 1931, 2321, 2334, 2729
AlwNI CAGNNNCTG 2 cut(s) 1022, 1688
Ama87I CYCGRG 1 cut(s) 2057
AoxI GGCC 6 cut(s) 316, 691, 704, 1350, 2053, 2352
ApeKI GCWGC 8 cut(s) 899, 902, 949, 952, 1337, 1898, 2284, 2595
ApoI RAATTY 4 cut(s) 1046, 1957, 2458, 2497
ArsI GACNNNNNNTTYG 2 cut(s) 1488, 1520
AsiGI ACCGGT 1 cut(s) 386
Asp700I GAANNNNTTC 1 cut(s) 721
Asp718I GGTACC 1 cut(s) 2793
AspLEI GCGC 1 cut(s) 2600
AspS9I GGNCC 5 cut(s) 635, 692, 2054, 2362, 2536
AsuC2I CCSGG 3 cut(s) 661, 2058, 2059
AvaI CYCGRG 1 cut(s) 2057
AvaII GGWCC 3 cut(s) 635, 2362, 2536
BaeGI GKGCMC 1 cut(s) 1819
BalI TGGCCA 3 cut(s) 318, 1352, 2354
BamHI GGATCC 1 cut(s) 2326
BanI GGYRCC 2 cut(s) 2710, 2793
BanII GRGCYC 2 cut(s) 590, 798
BauI CACGAG 1 cut(s) 2699
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 4 cut(s) 590, 798, 1224, 2269
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 8 cut(s) 889, 911, 961, 964, 1324, 1885, 2271, 2607
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 5 cut(s) 1148, 1587, 1863, 1941, 2716
BciVI GTATCC 1 cut(s) 2220
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 3 cut(s) 661, 2058, 2059
BcoDI GTCTC 5 cut(s) 59, 1013, 1232, 1489, 2605
BfaI CTAG 4 cut(s) 645, 1824, 1982, 2561
BfmI CTRYAG 1 cut(s) 1602
BfoI RGCGCY 1 cut(s) 2601
BfuAI ACCTGC 1 cut(s) 825
BfuI GTATCC 1 cut(s) 2220
BglII AGATCT 1 cut(s) 2382
BisI GCNGC 8 cut(s) 900, 903, 950, 953, 1338, 1899, 2285, 2596
BlsI GCNGC 8 cut(s) 901, 904, 951, 954, 1339, 1900, 2286, 2597
Bme1390I CCNGG 8 cut(s) 661, 1148, 1587, 1863, 1941, 2058, 2059, 2716
Bme18I GGWCC 3 cut(s) 635, 2362, 2536
BmeT110I CYCGRG 1 cut(s) 2057
BmgT120I GGNCC 5 cut(s) 635, 692, 2054, 2362, 2536
BmiI GGNNCC 5 cut(s) 2056, 2328, 2538, 2712, 2795
BmrFI CCNGG 8 cut(s) 661, 1148, 1587, 1863, 1941, 2058, 2059, 2716
BmrI ACTGGG 1 cut(s) 899
BmuI ACTGGG 1 cut(s) 899
BoxI GACNNNNGTC 1 cut(s) 2681
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 1532, 1564
Bpu10I CCTNAGC 2 cut(s) 459, 774
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 3 cut(s) 661, 2058, 2059
Bsa29I ATCGAT 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 1328
BsaBI GATNNNNATC 2 cut(s) 546, 990
BsaHI GRCGYC 1 cut(s) 2815
BsaI GGTCTC 2 cut(s) 59, 1489
BsaJI CCNNGG 9 cut(s) 69, 999, 1585, 1586, 2056, 2057, 2241, 2365, 2714
BsaWI WCCGGW 1 cut(s) 386
BsaXI ACNNNNNCTCC 2 cut(s) 2237, 2267
Bsc4I CCNNNNNNNGG 8 cut(s) 666, 1022, 1917, 1975, 1976, 2545, 2546, 2641
Bse118I RCCGGY 2 cut(s) 386, 702
Bse3DI GCAATG 3 cut(s) 2104, 2394, 2406
Bse8I GATNNNNATC 2 cut(s) 546, 990
BseBI CCWGG 5 cut(s) 1148, 1587, 1863, 1941, 2716
BseCI ATCGAT 1 cut(s) 223
BseDI CCNNGG 9 cut(s) 69, 999, 1585, 1586, 2056, 2057, 2241, 2365, 2714
BseJI GATNNNNATC 2 cut(s) 546, 990
BseLI CCNNNNNNNGG 8 cut(s) 666, 1022, 1917, 1975, 1976, 2545, 2546, 2641
BseMI GCAATG 3 cut(s) 2104, 2394, 2406
BseRI GAGGAG 2 cut(s) 123, 2291
BseSI GKGCMC 1 cut(s) 1819
BseXI GCAGC 8 cut(s) 889, 911, 961, 964, 1324, 1885, 2271, 2607
BseYI CCCAGC 2 cut(s) 2033, 2042
BsgI GTGCAG 2 cut(s) 888, 918
Bsh1285I CGRYCG 1 cut(s) 2682
BshFI GGCC 6 cut(s) 318, 693, 706, 1352, 2055, 2354
BshNI GGYRCC 2 cut(s) 2710, 2793
BshTI ACCGGT 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 2682
BsiHKAI GWGCWC 4 cut(s) 590, 798, 1224, 2269
BsiHKCI CYCGRG 1 cut(s) 2057
BsiSI CCGG 4 cut(s) 387, 660, 703, 2058
BslFI GGGAC 4 cut(s) 981, 1993, 2092, 2161
BslI CCNNNNNNNGG 8 cut(s) 666, 1022, 1917, 1975, 1976, 2545, 2546, 2641
BsmAI GTCTC 5 cut(s) 59, 1013, 1232, 1489, 2605
BsmFI GGGAC 4 cut(s) 981, 1993, 2092, 2161
BsnI GGCC 6 cut(s) 318, 693, 706, 1352, 2055, 2354
Bso31I GGTCTC 2 cut(s) 59, 1489
BsoBI CYCGRG 1 cut(s) 2057
Bsp1286I GDGCHC 5 cut(s) 590, 798, 1224, 1819, 2269
Bsp1407I TGTACA 2 cut(s) 1316, 2253
BspACI CCGC 6 cut(s) 1202, 1492, 2071, 2360, 2540, 2780
BspANI GGCC 6 cut(s) 318, 693, 706, 1352, 2055, 2354
BspDI ATCGAT 1 cut(s) 223
BspHI TCATGA 1 cut(s) 1435
BspLI GGNNCC 5 cut(s) 2056, 2328, 2538, 2712, 2795
BspMI ACCTGC 1 cut(s) 825
BspPI GGATC 9 cut(s) 312, 486, 993, 1001, 1844, 1931, 2321, 2334, 2729
BspQI GCTCTTC 1 cut(s) 578
BspT107I GGYRCC 2 cut(s) 2710, 2793
BspTNI GGTCTC 2 cut(s) 59, 1489
BsrDI GCAATG 3 cut(s) 2104, 2394, 2406
BsrFI RCCGGY 2 cut(s) 386, 702
BsrGI TGTACA 2 cut(s) 1316, 2253
BssAI RCCGGY 2 cut(s) 386, 702
BssECI CCNNGG 9 cut(s) 69, 999, 1585, 1586, 2056, 2057, 2241, 2365, 2714
BssNI GRCGYC 1 cut(s) 2815
BssSI CACGAG 1 cut(s) 2699
BssT1I CCWWGG 2 cut(s) 69, 2365
Bst2BI CACGAG 1 cut(s) 2699
Bst2UI CCWGG 5 cut(s) 1148, 1587, 1863, 1941, 2716
Bst4CI ACNGT 7 cut(s) 750, 885, 1400, 1568, 1663, 2258, 2680
Bst6I CTCTTC 9 cut(s) 21, 467, 578, 672, 707, 759, 1362, 1719, 2609
BstACI GRCGYC 1 cut(s) 2815
BstAPI GCANNNNNTGC 1 cut(s) 1165
BstAUI TGTACA 2 cut(s) 1316, 2253
BstBAI YACGTR 1 cut(s) 1328
BstC8I GCNNGC 6 cut(s) 704, 813, 1166, 1741, 2040, 2753
BstDSI CCRYGG 1 cut(s) 2241
BstH2I RGCGCY 1 cut(s) 2601
BstHHI GCGC 1 cut(s) 2600
BstMAI GTCTC 5 cut(s) 59, 1013, 1232, 1489, 2605
BstMCI CGRYCG 1 cut(s) 2682
BstMWI GCNNNNNNNGC 8 cut(s) 699, 949, 1165, 1480, 1489, 2039, 2044, 2525
BstNI CCWGG 5 cut(s) 1148, 1587, 1863, 1941, 2716
BstNSI RCATGY 4 cut(s) 1743, 2042, 2419, 2668
BstPAI GACNNNNGTC 1 cut(s) 2681
BstSCI CCNGG 8 cut(s) 659, 1146, 1585, 1861, 1939, 2056, 2057, 2714
BstSFI CTRYAG 1 cut(s) 1602
BstSLI GKGCMC 1 cut(s) 1819
BstV1I GCAGC 8 cut(s) 889, 911, 961, 964, 1324, 1885, 2271, 2607
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 5 cut(s) 985, 1006, 2326, 2382, 2734
BstXI CCANNNNNNTGG 3 cut(s) 379, 735, 1348
BstYI RGATCY 5 cut(s) 985, 1006, 2326, 2382, 2734
Bsu15I ATCGAT 1 cut(s) 223
BsuI GTATCC 1 cut(s) 2220
BsuRI GGCC 6 cut(s) 318, 693, 706, 1352, 2055, 2354
BsuTUI ATCGAT 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 2241
BtsI GCAGTG 1 cut(s) 2380
BveI ACCTGC 1 cut(s) 825
Cac8I GCNNGC 6 cut(s) 704, 813, 1166, 1741, 2040, 2753
CaiI CAGNNNCTG 2 cut(s) 1022, 1688
CciI TCATGA 1 cut(s) 1435
CfoI GCGC 1 cut(s) 2600
Cfr10I RCCGGY 2 cut(s) 386, 702
Cfr13I GGNCC 5 cut(s) 635, 692, 2054, 2362, 2536
Cfr9I CCCGGG 1 cut(s) 2057
ClaI ATCGAT 1 cut(s) 223
CseI GACGC 2 cut(s) 2750, 2804
CsiI ACCWGGT 1 cut(s) 1861
Csp6I GTAC 5 cut(s) 1317, 2049, 2254, 2662, 2794
CspAI ACCGGT 1 cut(s) 386
CviQI GTAC 5 cut(s) 1317, 2049, 2254, 2662, 2794
DraI TTTAAA 1 cut(s) 822
DrdI GACNNNNNNGTC 1 cut(s) 2420
DseDI GACNNNNNNGTC 1 cut(s) 2420
EaeI YGGCCR 4 cut(s) 316, 704, 1350, 2352
Eam1104I CTCTTC 9 cut(s) 21, 467, 578, 672, 707, 759, 1362, 1719, 2609
EarI CTCTTC 9 cut(s) 21, 467, 578, 672, 707, 759, 1362, 1719, 2609
Ecl136II GAGCTC 2 cut(s) 588, 796
Eco130I CCWWGG 2 cut(s) 69, 2365
Eco24I GRGCYC 2 cut(s) 590, 798
Eco31I GGTCTC 2 cut(s) 59, 1489
Eco47I GGWCC 3 cut(s) 635, 2362, 2536
Eco53kI GAGCTC 2 cut(s) 588, 796
Eco57I CTGAAG 5 cut(s) 893, 1202, 1386, 1496, 1710
Eco88I CYCGRG 1 cut(s) 2057
EcoICRI GAGCTC 2 cut(s) 588, 796
EcoO109I RGGNCCY 2 cut(s) 2054, 2536
EcoRII CCWGG 5 cut(s) 1146, 1585, 1861, 1939, 2714
EcoT14I CCWWGG 2 cut(s) 69, 2365
EcoT22I ATGCAT 1 cut(s) 1651
EcoT38I GRGCYC 2 cut(s) 590, 798
ErhI CCWWGG 2 cut(s) 69, 2365
FaqI GGGAC 4 cut(s) 981, 1993, 2092, 2161
FauI CCCGC 1 cut(s) 2547
FauNDI CATATG 1 cut(s) 1072
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 2685
Fnu4HI GCNGC 8 cut(s) 900, 903, 950, 953, 1338, 1899, 2285, 2596
FriOI GRGCYC 2 cut(s) 590, 798
Fsp4HI GCNGC 8 cut(s) 900, 903, 950, 953, 1338, 1899, 2285, 2596
FspBI CTAG 4 cut(s) 645, 1824, 1982, 2561
GlaI GCGC 1 cut(s) 2599
GluI GCNGC 8 cut(s) 900, 903, 950, 953, 1338, 1899, 2285, 2596
GsaI CCCAGC 2 cut(s) 2037, 2046
HaeII RGCGCY 1 cut(s) 2601
HaeIII GGCC 6 cut(s) 318, 693, 706, 1352, 2055, 2354
HapII CCGG 4 cut(s) 387, 660, 703, 2058
HgaI GACGC 2 cut(s) 2750, 2804
HhaI GCGC 1 cut(s) 2600
Hin1I GRCGYC 1 cut(s) 2815
Hin6I GCGC 1 cut(s) 2598
HinP1I GCGC 1 cut(s) 2598
HincII GTYRAC 1 cut(s) 2686
HindII GTYRAC 1 cut(s) 2686
HindIII AAGCTT 1 cut(s) 1481
HinfI GANTC 6 cut(s) 33, 427, 874, 971, 1985, 2404
HpaII CCGG 4 cut(s) 387, 660, 703, 2058
Hpy166II GTNNAC 3 cut(s) 915, 1319, 2686
Hpy188I TCNGA 7 cut(s) 331, 432, 519, 592, 763, 1003, 2409
Hpy8I GTNNAC 3 cut(s) 915, 1319, 2686
Hpy99I CGWCG 2 cut(s) 1229, 2687
HpyCH4III ACNGT 7 cut(s) 750, 885, 1400, 1568, 1663, 2258, 2680
HpyCH4IV ACGT 2 cut(s) 1327, 2770
HpyF10VI GCNNNNNNNGC 8 cut(s) 699, 949, 1165, 1480, 1489, 2039, 2044, 2525
HpySE526I ACGT 2 cut(s) 1327, 2770
Hsp92I GRCGYC 1 cut(s) 2815
HspAI GCGC 1 cut(s) 2598
KpnI GGTACC 1 cut(s) 2797
KroI GCCGGC 1 cut(s) 702
KroNI GCCGGC 1 cut(s) 704
Ksp22I TGATCA 1 cut(s) 547
LguI GCTCTTC 1 cut(s) 578
LmnI GCTCC 4 cut(s) 770, 928, 2245, 2264
Lsp1109I GCAGC 8 cut(s) 889, 911, 961, 964, 1324, 1885, 2271, 2607
MabI ACCWGGT 1 cut(s) 1861
MaeI CTAG 4 cut(s) 645, 1824, 1982, 2561
MaeII ACGT 2 cut(s) 1327, 2770
MfeI CAATTG 1 cut(s) 2249
MflI RGATCY 5 cut(s) 985, 1006, 2326, 2382, 2734
MhlI GDGCHC 5 cut(s) 590, 798, 1224, 1819, 2269
MlsI TGGCCA 3 cut(s) 318, 1352, 2354
MluNI TGGCCA 3 cut(s) 318, 1352, 2354
MlyI GAGTC 2 cut(s) 42, 436
MmeI TCCRAC 2 cut(s) 42, 948
Mox20I TGGCCA 3 cut(s) 318, 1352, 2354
Mph1103I ATGCAT 1 cut(s) 1651
MroNI GCCGGC 1 cut(s) 702
MroXI GAANNNNTTC 1 cut(s) 721
MscI TGGCCA 3 cut(s) 318, 1352, 2354
MseI TTAA 6 cut(s) 270, 413, 821, 870, 924, 1679
MslI CAYNNNNRTG 7 cut(s) 186, 330, 1298, 1346, 1455, 2399, 2744
Msp20I TGGCCA 3 cut(s) 318, 1352, 2354
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 4 cut(s) 387, 660, 703, 2058
MspR9I CCNGG 8 cut(s) 661, 1148, 1587, 1863, 1941, 2058, 2059, 2716
MunI CAATTG 1 cut(s) 2249
MvaI CCWGG 5 cut(s) 1148, 1587, 1863, 1941, 2716
MwoI GCNNNNNNNGC 8 cut(s) 699, 949, 1165, 1480, 1489, 2039, 2044, 2525
NaeI GCCGGC 1 cut(s) 704
NciI CCSGG 3 cut(s) 661, 2058, 2059
NdeI CATATG 1 cut(s) 1072
NgoMIV GCCGGC 1 cut(s) 702
NlaIV GGNNCC 5 cut(s) 2056, 2328, 2538, 2712, 2795
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 6 cut(s) 852, 885, 1516, 1634, 1694, 2674
NsiI ATGCAT 1 cut(s) 1651
NspI RCATGY 4 cut(s) 1743, 2042, 2419, 2668
PaeI GCATGC 2 cut(s) 1743, 2042
PagI TCATGA 1 cut(s) 1435
PasI CCCWGGG 1 cut(s) 1586
PciI ACATGT 1 cut(s) 2415
PciSI GCTCTTC 1 cut(s) 578
PdiI GCCGGC 1 cut(s) 704
PdmI GAANNNNTTC 1 cut(s) 721
PfeI GAWTC 4 cut(s) 874, 971, 1985, 2404
PflMI CCANNNNNTGG 2 cut(s) 1022, 2641
PfoI TCCNGGA 2 cut(s) 659, 1146
PinAI ACCGGT 1 cut(s) 386
PkrI GCNGC 8 cut(s) 901, 904, 951, 954, 1339, 1900, 2286, 2597
PleI GAGTC 2 cut(s) 41, 435
PpsI GAGTC 2 cut(s) 41, 435
Ppu21I YACGTR 1 cut(s) 1328
PpuMI RGGWCCY 1 cut(s) 2536
PscI ACATGT 1 cut(s) 2415
PshAI GACNNNNGTC 1 cut(s) 2681
PsiI TTATAA 3 cut(s) 186, 357, 1965
Psp124BI GAGCTC 2 cut(s) 590, 798
Psp5II RGGWCCY 1 cut(s) 2536
Psp6I CCWGG 5 cut(s) 1146, 1585, 1861, 1939, 2714
PspFI CCCAGC 2 cut(s) 2033, 2042
PspGI CCWGG 5 cut(s) 1146, 1585, 1861, 1939, 2714
PspN4I GGNNCC 5 cut(s) 2056, 2328, 2538, 2712, 2795
PspPI GGNCC 5 cut(s) 635, 692, 2054, 2362, 2536
PspPPI RGGWCCY 1 cut(s) 2536
PstNI CAGNNNCTG 2 cut(s) 1022, 1688
PsuI RGATCY 5 cut(s) 985, 1006, 2326, 2382, 2734
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 5 cut(s) 1318, 2050, 2255, 2663, 2795
RsaNI GTAC 5 cut(s) 1317, 2049, 2254, 2662, 2794
RseI CAYNNNNRTG 7 cut(s) 186, 330, 1298, 1346, 1455, 2399, 2744
SacI GAGCTC 2 cut(s) 590, 798
SalI GTCGAC 1 cut(s) 2684
SapI GCTCTTC 1 cut(s) 578
SaqAI TTAA 6 cut(s) 270, 413, 821, 870, 924, 1679
SatI GCNGC 8 cut(s) 900, 903, 950, 953, 1338, 1899, 2285, 2596
Sau96I GGNCC 5 cut(s) 635, 692, 2054, 2362, 2536
SchI GAGTC 2 cut(s) 42, 436
ScrFI CCNGG 8 cut(s) 661, 1148, 1587, 1863, 1941, 2058, 2059, 2716
SduI GDGCHC 5 cut(s) 590, 798, 1224, 1819, 2269
SexAI ACCWGGT 1 cut(s) 1861
SfcI CTRYAG 1 cut(s) 1602
SinI GGWCC 3 cut(s) 635, 2362, 2536
SmaI CCCGGG 1 cut(s) 2059
SmiMI CAYNNNNRTG 7 cut(s) 186, 330, 1298, 1346, 1455, 2399, 2744
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
SphI GCATGC 2 cut(s) 1743, 2042
SsiI CCGC 6 cut(s) 1202, 1492, 2071, 2360, 2540, 2780
SspI AATATT 2 cut(s) 1409, 1894
SspMI CTAG 4 cut(s) 645, 1824, 1982, 2561
SstI GAGCTC 2 cut(s) 590, 798
StyD4I CCNGG 8 cut(s) 659, 1146, 1585, 1861, 1939, 2056, 2057, 2714
StyI CCWWGG 2 cut(s) 69, 2365
TaaI ACNGT 7 cut(s) 750, 885, 1400, 1568, 1663, 2258, 2680
TaiI ACGT 2 cut(s) 1330, 2773
TaqI TCGA 3 cut(s) 223, 533, 2685
TatI WGTACW 3 cut(s) 1316, 2253, 2661
TfiI GAWTC 4 cut(s) 874, 971, 1985, 2404
Tru1I TTAA 6 cut(s) 270, 413, 821, 870, 924, 1679
Tru9I TTAA 6 cut(s) 270, 413, 821, 870, 924, 1679
TseFI GTSAC 6 cut(s) 852, 885, 1516, 1634, 1694, 2674
TseI GCWGC 8 cut(s) 899, 902, 949, 952, 1337, 1898, 2284, 2595
Tsp45I GTSAC 6 cut(s) 852, 885, 1516, 1634, 1694, 2674
TspDTI ATGAA 7 cut(s) 267, 348, 1443, 1778, 2180, 2652, 2814
TspGWI ACGGA 1 cut(s) 2258
TspMI CCCGGG 1 cut(s) 2057
Van91I CCANNNNNTGG 2 cut(s) 1022, 2641
VpaK11BI GGWCC 3 cut(s) 635, 2362, 2536
XapI RAATTY 4 cut(s) 1046, 1957, 2458, 2497
XceI RCATGY 4 cut(s) 1743, 2042, 2419, 2668
XcmI CCANNNNNNNNNTGG 1 cut(s) 2698
XmaI CCCGGG 1 cut(s) 2057
XmiI GTMKAC 1 cut(s) 2685
XmnI GAANNNNTTC 1 cut(s) 721
XspI CTAG 4 cut(s) 645, 1824, 1982, 2561
Zsp2I ATGCAT 1 cut(s) 1651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.