Rh4AG097800

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000671
Physical Location & Seq
Reverse (-)
21049285 .. 21053748
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG097800.1

Sequence Viewer

Length: 3579 bp
ATGGCTGCAATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAAGTCTGTTCAGAGCTGGAAACCCATCTAGGGTTTGGGGATAAAGTTCTTGCTGAGTTCATAACTGAGATGGGACGAAACTGTGGGAATGTGGATGAATTTGATGCCAAATTGAAGGAGAACGGTGCCGAGATGCCCGATTACTTTGTCCGCACACTCCTCACTATCATTCATGCAATTCTTCCTCCGAAGCCAAAGTCTGAGGATGACTCAATGATAGAGAGTACTACTGAAGGTAAGAAGACCAAGTTTAAGGCTCTGTCTATTGCTGATAACAGGGATAGGGTAAATGATCTCCAGAGGGAAATTGAGATGGAGACCAAAGAAAAGAAAAATGACCGAGAAGAGTGGGGTGATGAGCGTGAAGAAGATAGGCGTAGAGGTAGGGGTAGAGGTAGAGATAGAGACAGATATGGGGAAAGAGATAGAGACAGAGATAGAGATAGGGATAGGAGAGAGAGGGGCAGGGATAGGTATGACAGAGATGAGAGGCGTAGAGATACACATCATGATTATGATAAGGGAAGGCATACAGACCGATCCAATAAGCACAGAAGGGATGGGTATCAAGAGGATGAAGATGTTAGAGAAGATGGTGGTAACAGGAGAGGAGGTGGGGATCAACGGAGTGGACTGCACCATTCTGATGAGCCTGAATTGTATCAGGTTTATAAGGGCAGGGTATCAAAAGTCATGGACACGGGTTGCTTTGTTCAGTTCAGTGACTTTAGGGGGAAGGAGGGTTTGGTTCATGTTTCACAGATGGCAACGCGGCGTATTGGTAACGCTAAAGATGTGGTGAAGAGGGATCAAGAGGTCTATGTTAAGGTGATTTCAGTTTCTGGTCAGAAGTTGAGCCTTTCAATGAGGGATGTTGATCAGCATACTGGAAAGGATTTGCTTCCATTGAAGAAGAGCTCAGAGGATGATGCTCATAGGACAAACCCTTCGGTATCAAAGCATGATGGGCCTGTGACAAGGACTGGTCTTTCTGGGATTAGAATTGTGGAAGAGAACAATGTTGCCCCTTCTCGGCGGCCATTAAAGAGAATGAGCTCGCCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCAGGTGTTTTGGGTGTTACAGAATATCCAATGTATGATGAGGAAACAGAGGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAGCTTGAAATTGAGCTTAATGAGGATGAGCCAGCCTTTTTGCAAGGGCAAAGCAGGTATTCTGTAGATATGTCACCTGTGAAAATCTTTAAGAATCCAGAGGGGTCATTAAGTCGTGCAGCTGCACTTCAATCTGCACTTATTAAAGAGCGCAGAGAAGTGCGTGAGCAGCAGCAACGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAGCTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACTGTCAGTTTTGGGCAGAGGTCAAAGCTCTCACTTCAGGAACAGAGGCAGAGCTTGCCTATCTACAAGCTCAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCGGGCAAGACAACCCAGGTAACACAATATCTTGCAGAAGCAGGTTACACAACAATGGGTAAGATTGGGTGTACACAACCACGTAGGGTGGCCGCGATGTCTGTAGCCAAGAGAGTTGCTGAAGAATTTGGTTGTCGTTTGGGGGAGGAAGTTGGCTATGCTATTCGTTTTGAGGATTGCACTGGCCCTGATACTGTCATCAAGTACATGACTGATGGTATGCTTCTTAGGGAGATTTTGATCGATGAAAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGCGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTATCAAGCGGAGACCAGACCTACGTTTGATTGTCACATCGGCTACCTTAGATGCTGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATCTTCACTATACCGGGGAGAACTTTTCCTGTAGAGATACTATACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTCTAATCACTGTTCTACAGATTCACTTAACAGAACCAGAAGGGGACATCCTTCTCTTCTTGACGGGACAGGAAGAAATTGATTTCTCATGCCAGTCACTTTTTGAGAGGATGAAAGGTCTTGGTAAAAATGTACCTGAGTTGATTATCTTACCAGTTTACAGTGCCCTTCCTAGTGAAATGCAGTCTAGGATTTTTGATCCAGCTCCACCTGGTAAGAGGAAAGTAGTTGTGGCTACTAATATTGCTGAGGCATCACTAACCATTGATGGGATATTTTATGTAATTGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAAGGGCTGGATTCACTGGTCATAACCCCAATTTCACAAGCATCAGCCAAGCAAAGAGCTGGGCGCGCTGGGCGTACTGGGCCTGGAAAGTGTTACCGCCTCTACACTGAGAGTGCATACCGCAACGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGTTAATTACACTTACCATGAAAGCAATGGGGATAAACGATCTGTTGTCTTTTGATTTTATGGATCCTCCCTCACCCCAAGCACTCATTTCTGCTATGGAACAACTGTACAGTTTAGGAGCACTCGATGAGGAGGGGCTTCTCACCAAACTGGGTAGGAAAATGGCTGAGTTTCCTCTTGATCCACCACTATCTAAGATGCTACTAGCCAGTGTGGACCTTGGATGCAGTGATGAGATCTTGACCATGATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTGTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCATGGTGCTTTGAGAACTTTGTTCAGTCTCGATCCCTTAGGAGGGCACAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGATGTTGTGAGTGCTGGAAAGAATTTTACACAGATCAGGAAGGCAATTACGGCGGGATTTTTCTTCCATGGTGCTAGAAAGGACCCTCAGGAGGGGTATAGAACCTTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCGCTCTTCCAGAGACAACCAGATTGGATCATCTACCATGAGTTGGTTATGACTACGAAGGAGTATATGCGTGAGGTAACCGTCGTTGATCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCAAGGTGGCAGACCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAGCGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCTTGGCGTCTTAGTAAACGACGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1192

Amino Acids

136.19

Weight (kDa)

6.35

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 237 - 307 1.4e-11 S1 RNA binding domain
DEAD PF00270 546 - 695 1.2e-07 DEAD/DEAH box helicase
AAA_22 PF13401 554 - 679 2.9e-06 AAA domain
Helicase_C PF00271 739 - 869 8.8e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 931 - 959 8.2e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 960 - 1019 3e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1077 - 1153 8.4e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 726, 2517
Acc36I ACCTGC 2 cut(s) 1275, 1727
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1472, 3382
AccBSI CCGCTC 1 cut(s) 1990
AccII CGCG 3 cut(s) 826, 1790, 2592
AcoI YGGCCR 2 cut(s) 1091, 1785
AcsI RAATTY 4 cut(s) 152, 1820, 2679, 3217
AcuI CTGAAG 4 cut(s) 306, 1580, 1836, 2652
AcyI GRCGYC 1 cut(s) 3556
AfaI GTAC 8 cut(s) 38, 280, 1768, 1901, 1958, 2334, 2602, 2807
AjiI CACGTC 1 cut(s) 3572
AjnI CCWGG 6 cut(s) 1710, 2410, 2491, 2608, 3000, 3455
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
Alw21I GWGCWC 3 cut(s) 974, 1112, 2821
Alw26I GTCTC 9 cut(s) 365, 453, 477, 1463, 1682, 2038, 3036, 3132, 3346
AlwNI CAGNNNCTG 2 cut(s) 896, 1472
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 8 cut(s) 1022, 1091, 1518, 1785, 1879, 2606, 2981, 3098
ApeKI GCWGC 6 cut(s) 5, 1349, 1352, 1399, 1402, 2173
ApoI RAATTY 4 cut(s) 152, 1820, 2679, 3217
ArsI GACNNNNNNTTYG 4 cut(s) 236, 268, 985, 1017
Asp700I GAANNNNTTC 1 cut(s) 1644
AspA2I CCTAGG 1 cut(s) 2984
AspLEI GCGC 5 cut(s) 1383, 2592, 2594, 3343, 3498
AspS9I GGNCC 6 cut(s) 1022, 1880, 2606, 2914, 3098, 3277
AsuC2I CCSGG 1 cut(s) 2136
AsuHPI GGTGA 8 cut(s) 419, 865, 895, 1296, 1487, 1697, 2763, 2833
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 2914, 3277
AvrII CCTAGG 1 cut(s) 2984
AxyI CCTNAGG 3 cut(s) 1147, 3137, 3282
BaeGI GKGCMC 2 cut(s) 2368, 3148
BamHI GGATCC 1 cut(s) 2761
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 974, 1112
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 302, 1565
Bbv12I GWGCWC 3 cut(s) 974, 1112, 2821
BbvCI CCTCAGC 2 cut(s) 1224, 2448
BbvI GCAGC 5 cut(s) 1339, 1361, 1411, 1414, 2185
BceAI ACGGC 1 cut(s) 3261
BciT130I CCWGG 6 cut(s) 1712, 2412, 2493, 2610, 3002, 3457
BclI TGATCA 2 cut(s) 931, 3007
BcnI CCSGG 1 cut(s) 2136
BcoDI GTCTC 9 cut(s) 365, 453, 477, 1463, 1682, 2038, 3036, 3132, 3346
BfaI CTAG 7 cut(s) 83, 2373, 2388, 2903, 2985, 3270, 3449
BfmI CTRYAG 4 cut(s) 1293, 1797, 2151, 2216
BfuAI ACCTGC 2 cut(s) 1275, 1727
BglII AGATCT 1 cut(s) 2934
BisI GCNGC 9 cut(s) 6, 827, 1091, 1350, 1353, 1400, 1403, 1788, 2174
BlnI CCTAGG 1 cut(s) 2984
BlsI GCNGC 9 cut(s) 7, 828, 1092, 1351, 1354, 1401, 1404, 1789, 2175
BmcAI AGTACT 2 cut(s) 280, 1958
Bme1390I CCNGG 7 cut(s) 1712, 2136, 2412, 2493, 2610, 3002, 3457
Bme18I GGWCC 2 cut(s) 2914, 3277
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 3572
BmgT120I GGNCC 6 cut(s) 1022, 1880, 2606, 2914, 3098, 3277
BmiI GGNNCC 3 cut(s) 181, 2763, 3279
BmrFI CCNGG 7 cut(s) 1712, 2136, 2412, 2493, 2610, 3002, 3457
BmrI ACTGGG 2 cut(s) 2613, 2858
BmuI ACTGGG 2 cut(s) 2613, 2858
BpiI GAAGAC 2 cut(s) 302, 1565
BplI GAGNNNNNCTC 2 cut(s) 248, 280
BpmI CTGGAG 1 cut(s) 335
Bpu10I CCTNAGC 2 cut(s) 1224, 2448
BpuEI CTTGAG 1 cut(s) 1616
BpuMI CCSGG 1 cut(s) 2136
Bsa29I ATCGAT 1 cut(s) 1938
BsaAI YACGTR 1 cut(s) 1778
BsaBI GATNNNNATC 5 cut(s) 558, 618, 930, 1440, 1934
BsaHI GRCGYC 1 cut(s) 3556
BsaI GGTCTC 3 cut(s) 365, 2038, 3036
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bse21I CCTNAGG 3 cut(s) 1147, 3137, 3282
Bse3DI GCAATG 1 cut(s) 2730
Bse8I GATNNNNATC 5 cut(s) 558, 618, 930, 1440, 1934
BseBI CCWGG 6 cut(s) 1712, 2412, 2493, 2610, 3002, 3457
BseCI ATCGAT 1 cut(s) 1938
BseJI GATNNNNATC 5 cut(s) 558, 618, 930, 1440, 1934
BseMI GCAATG 1 cut(s) 2730
BsePI GCGCGC 1 cut(s) 2590
BseRI GAGGAG 3 cut(s) 203, 678, 2843
BseSI GKGCMC 2 cut(s) 2368, 3148
BseXI GCAGC 5 cut(s) 1339, 1361, 1411, 1414, 2185
BseYI CCCAGC 2 cut(s) 2585, 2594
BsgI GTGCAG 4 cut(s) 674, 1338, 1350, 1368
Bsh1236I CGCG 3 cut(s) 826, 1790, 2592
BshFI GGCC 8 cut(s) 1024, 1093, 1520, 1787, 1881, 2608, 2983, 3100
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 1 cut(s) 1938
BsiHKAI GWGCWC 3 cut(s) 974, 1112, 2821
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 2 cut(s) 1115, 2135
BslFI GGGAC 4 cut(s) 141, 2258, 2280, 2644
BsmAI GTCTC 9 cut(s) 365, 453, 477, 1463, 1682, 2038, 3036, 3132, 3346
BsmFI GGGAC 4 cut(s) 141, 2258, 2280, 2644
BsnI GGCC 8 cut(s) 1024, 1093, 1520, 1787, 1881, 2608, 2983, 3100
Bso31I GGTCTC 3 cut(s) 365, 2038, 3036
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 974, 1112, 2368, 2821, 3148
Bsp1407I TGTACA 2 cut(s) 1766, 2805
Bsp19I CCATGG 2 cut(s) 3101, 3262
BspANI GGCC 8 cut(s) 1024, 1093, 1520, 1787, 1881, 2608, 2983, 3100
BspDI ATCGAT 1 cut(s) 1938
BspFNI CGCG 3 cut(s) 826, 1790, 2592
BspHI TCATGA 2 cut(s) 562, 1984
BspLI GGNNCC 3 cut(s) 181, 2763, 3279
BspMI ACCTGC 2 cut(s) 1275, 1727
BspQI GCTCTTC 3 cut(s) 962, 1224, 3350
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 365, 2038, 3036
BsrBI CCGCTC 1 cut(s) 1990
BsrDI GCAATG 1 cut(s) 2730
BsrGI TGTACA 2 cut(s) 1766, 2805
BssHII GCGCGC 1 cut(s) 2590
BssNI GRCGYC 1 cut(s) 3556
BssT1I CCWWGG 6 cut(s) 45, 1448, 2917, 2984, 3101, 3262
Bst2UI CCWGG 6 cut(s) 1712, 2412, 2493, 2610, 3002, 3457
Bst6I CTCTTC 9 cut(s) 393, 851, 962, 1059, 1209, 1224, 2261, 3008, 3350
BstACI GRCGYC 1 cut(s) 3556
BstAPI GCANNNNNTGC 1 cut(s) 1615
BstAUI TGTACA 2 cut(s) 1766, 2805
BstBAI YACGTR 1 cut(s) 1778
BstC8I GCNNGC 5 cut(s) 1112, 1263, 1616, 2592, 3500
BstDSI CCRYGG 2 cut(s) 3101, 3262
BstEII GGTNACC 1 cut(s) 3415
BstFNI CGCG 3 cut(s) 826, 1790, 2592
BstHHI GCGC 5 cut(s) 1383, 2592, 2594, 3343, 3498
BstMAI GTCTC 9 cut(s) 365, 453, 477, 1463, 1682, 2038, 3036, 3132, 3346
BstMWI GCNNNNNNNGC 9 cut(s) 1021, 1399, 1615, 2591, 2596, 2605, 3080, 3245, 3508
BstNI CCWGG 6 cut(s) 1712, 2412, 2493, 2610, 3002, 3457
BstNSI RCATGY 1 cut(s) 1531
BstPI GGTNACC 1 cut(s) 3415
BstSCI CCNGG 7 cut(s) 1710, 2134, 2410, 2491, 2608, 3000, 3455
BstSFI CTRYAG 4 cut(s) 1293, 1797, 2151, 2216
BstSLI GKGCMC 2 cut(s) 2368, 3148
BstUI CGCG 3 cut(s) 826, 1790, 2592
BstV1I GCAGC 5 cut(s) 1339, 1361, 1411, 1414, 2185
BstV2I GAAGAC 2 cut(s) 302, 1565
BstX2I RGATCY 4 cut(s) 1435, 1456, 2761, 2934
BstXI CCANNNNNNTGG 1 cut(s) 3552
BstYI RGATCY 4 cut(s) 1435, 1456, 2761, 2934
Bsu15I ATCGAT 1 cut(s) 1938
Bsu36I CCTNAGG 3 cut(s) 1147, 3137, 3282
BsuRI GGCC 8 cut(s) 1024, 1093, 1520, 1787, 1881, 2608, 2983, 3100
BsuTUI ATCGAT 1 cut(s) 1938
BtgI CCRYGG 2 cut(s) 3101, 3262
BtgZI GCGATG 1 cut(s) 1805
BtrI CACGTC 1 cut(s) 3572
BtsI GCAGTG 4 cut(s) 1659, 2932, 3069, 3343
BveI ACCTGC 2 cut(s) 1275, 1727
Cac8I GCNNGC 5 cut(s) 1112, 1263, 1616, 2592, 3500
CaiI CAGNNNCTG 2 cut(s) 896, 1472
CciI TCATGA 2 cut(s) 562, 1984
CfoI GCGC 5 cut(s) 1383, 2592, 2594, 3343, 3498
Cfr13I GGNCC 6 cut(s) 1022, 1880, 2606, 2914, 3098, 3277
ClaI ATCGAT 1 cut(s) 1938
CseI GACGC 2 cut(s) 3491, 3545
CsiI ACCWGGT 1 cut(s) 2410
Csp6I GTAC 8 cut(s) 37, 279, 1767, 1900, 1957, 2333, 2601, 2806
CviQI GTAC 8 cut(s) 37, 279, 1767, 1900, 1957, 2333, 2601, 2806
EaeI YGGCCR 2 cut(s) 1091, 1785
Eam1104I CTCTTC 9 cut(s) 393, 851, 962, 1059, 1209, 1224, 2261, 3008, 3350
EarI CTCTTC 9 cut(s) 393, 851, 962, 1059, 1209, 1224, 2261, 3008, 3350
Ecl136II GAGCTC 2 cut(s) 972, 1110
Eco130I CCWWGG 6 cut(s) 45, 1448, 2917, 2984, 3101, 3262
Eco147I AGGCCT 1 cut(s) 2983
Eco24I GRGCYC 2 cut(s) 974, 1112
Eco31I GGTCTC 3 cut(s) 365, 2038, 3036
Eco47I GGWCC 2 cut(s) 2914, 3277
Eco53kI GAGCTC 2 cut(s) 972, 1110
Eco57I CTGAAG 4 cut(s) 306, 1580, 1836, 2652
Eco81I CCTNAGG 3 cut(s) 1147, 3137, 3282
Eco88I CYCGRG 1 cut(s) 32
Eco91I GGTNACC 1 cut(s) 3415
EcoICRI GAGCTC 2 cut(s) 972, 1110
EcoO109I RGGNCCY 1 cut(s) 3277
EcoO65I GGTNACC 1 cut(s) 3415
EcoRII CCWGG 6 cut(s) 1710, 2410, 2491, 2608, 3000, 3455
EcoT14I CCWWGG 6 cut(s) 45, 1448, 2917, 2984, 3101, 3262
EcoT22I ATGCAT 1 cut(s) 2200
EcoT38I GRGCYC 2 cut(s) 974, 1112
ErhI CCWWGG 6 cut(s) 45, 1448, 2917, 2984, 3101, 3262
FaqI GGGAC 4 cut(s) 141, 2258, 2280, 2644
FauI CCCGC 1 cut(s) 3241
FbaI TGATCA 2 cut(s) 931, 3007
Fnu4HI GCNGC 9 cut(s) 6, 827, 1091, 1350, 1353, 1400, 1403, 1788, 2174
FriOI GRGCYC 2 cut(s) 974, 1112
Fsp4HI GCNGC 9 cut(s) 6, 827, 1091, 1350, 1353, 1400, 1403, 1788, 2174
FspBI CTAG 7 cut(s) 83, 2373, 2388, 2903, 2985, 3270, 3449
GlaI GCGC 5 cut(s) 1382, 2591, 2593, 3342, 3497
GluI GCNGC 9 cut(s) 6, 827, 1091, 1350, 1353, 1400, 1403, 1788, 2174
GsaI CCCAGC 2 cut(s) 2589, 2598
GsuI CTGGAG 1 cut(s) 335
HaeIII GGCC 8 cut(s) 1024, 1093, 1520, 1787, 1881, 2608, 2983, 3100
HapII CCGG 2 cut(s) 1115, 2135
HgaI GACGC 2 cut(s) 3491, 3545
HhaI GCGC 5 cut(s) 1383, 2592, 2594, 3343, 3498
Hin1I GRCGYC 1 cut(s) 3556
Hin6I GCGC 5 cut(s) 1381, 2590, 2592, 3341, 3496
HinP1I GCGC 5 cut(s) 1381, 2590, 2592, 3341, 3496
HinfI GANTC 7 cut(s) 263, 1324, 1421, 1441, 2221, 2537, 2956
HpaII CCGG 2 cut(s) 1115, 2135
HphI GGTGA 8 cut(s) 419, 865, 895, 1296, 1487, 1697, 2763, 2833
Hpy166II GTNNAC 6 cut(s) 686, 1767, 1769, 2359, 2914, 3566
Hpy8I GTNNAC 6 cut(s) 686, 1767, 1769, 2359, 2914, 3566
Hpy99I CGWCG 2 cut(s) 3425, 3573
HpyCH4IV ACGT 3 cut(s) 1777, 2056, 3571
HpyF10VI GCNNNNNNNGC 9 cut(s) 1021, 1399, 1615, 2591, 2596, 2605, 3080, 3245, 3508
HpySE526I ACGT 3 cut(s) 1777, 2056, 3571
Hsp92I GRCGYC 1 cut(s) 3556
HspAI GCGC 5 cut(s) 1381, 2590, 2592, 3341, 3496
Ksp22I TGATCA 2 cut(s) 931, 3007
LguI GCTCTTC 3 cut(s) 962, 1224, 3350
LmnI GCTCC 4 cut(s) 1220, 1495, 2410, 2816
Lsp1109I GCAGC 5 cut(s) 1339, 1361, 1411, 1414, 2185
MabI ACCWGGT 1 cut(s) 2410
MaeI CTAG 7 cut(s) 83, 2373, 2388, 2903, 2985, 3270, 3449
MaeII ACGT 3 cut(s) 1777, 2056, 3571
MbiI CCGCTC 1 cut(s) 1990
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 4 cut(s) 1435, 1456, 2761, 2934
MhlI GDGCHC 5 cut(s) 974, 1112, 2368, 2821, 3148
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 1398
Mph1103I ATGCAT 1 cut(s) 2200
MroXI GAANNNNTTC 1 cut(s) 1644
MseI TTAA 9 cut(s) 306, 879, 1097, 1248, 1320, 1340, 1374, 2228, 2702
MslI CAYNNNNRTG 4 cut(s) 567, 699, 1748, 2004
MspA1I CMGCKG 1 cut(s) 1352
MspI CCGG 2 cut(s) 1115, 2135
MspR9I CCNGG 7 cut(s) 1712, 2136, 2412, 2493, 2610, 3002, 3457
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 6 cut(s) 1712, 2412, 2493, 2610, 3002, 3457
MvnI CGCG 3 cut(s) 826, 1790, 2592
MwoI GCNNNNNNNGC 9 cut(s) 1021, 1399, 1615, 2591, 2596, 2605, 3080, 3245, 3508
NciI CCSGG 1 cut(s) 2136
NcoI CCATGG 2 cut(s) 3101, 3262
NlaIV GGNNCC 3 cut(s) 181, 2763, 3279
NmeAIII GCCGAG 2 cut(s) 208, 1066
NmuCI GTSAC 5 cut(s) 776, 1027, 1302, 2065, 2295
NsiI ATGCAT 1 cut(s) 2200
NspI RCATGY 1 cut(s) 1531
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 562, 1984
PauI GCGCGC 1 cut(s) 2590
PceI AGGCCT 1 cut(s) 2983
PciSI GCTCTTC 3 cut(s) 962, 1224, 3350
PdmI GAANNNNTTC 1 cut(s) 1644
PfeI GAWTC 6 cut(s) 1324, 1421, 1441, 2221, 2537, 2956
PflMI CCANNNNNTGG 2 cut(s) 1472, 3382
PfoI TCCNGGA 1 cut(s) 2491
PkrI GCNGC 9 cut(s) 7, 828, 1092, 1351, 1354, 1401, 1404, 1789, 2175
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 1778
PpuMI RGGWCCY 1 cut(s) 3277
PsiI TTATAA 2 cut(s) 726, 2517
Psp124BI GAGCTC 2 cut(s) 974, 1112
Psp5II RGGWCCY 1 cut(s) 3277
Psp6I CCWGG 6 cut(s) 1710, 2410, 2491, 2608, 3000, 3455
PspEI GGTNACC 1 cut(s) 3415
PspFI CCCAGC 2 cut(s) 2585, 2594
PspGI CCWGG 6 cut(s) 1710, 2410, 2491, 2608, 3000, 3455
PspN4I GGNNCC 3 cut(s) 181, 2763, 3279
PspPI GGNCC 6 cut(s) 1022, 1880, 2606, 2914, 3098, 3277
PspPPI RGGWCCY 1 cut(s) 3277
PspXI VCTCGAGB 1 cut(s) 32
PstNI CAGNNNCTG 2 cut(s) 896, 1472
PsuI RGATCY 4 cut(s) 1435, 1456, 2761, 2934
PteI GCGCGC 1 cut(s) 2590
PvuII CAGCTG 1 cut(s) 1352
RsaI GTAC 8 cut(s) 38, 280, 1768, 1901, 1958, 2334, 2602, 2807
RsaNI GTAC 8 cut(s) 37, 279, 1767, 1900, 1957, 2333, 2601, 2806
RseI CAYNNNNRTG 4 cut(s) 567, 699, 1748, 2004
SacI GAGCTC 2 cut(s) 974, 1112
SapI GCTCTTC 3 cut(s) 962, 1224, 3350
SaqAI TTAA 9 cut(s) 306, 879, 1097, 1248, 1320, 1340, 1374, 2228, 2702
SatI GCNGC 9 cut(s) 6, 827, 1091, 1350, 1353, 1400, 1403, 1788, 2174
Sau96I GGNCC 6 cut(s) 1022, 1880, 2606, 2914, 3098, 3277
ScaI AGTACT 2 cut(s) 280, 1958
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 7 cut(s) 1712, 2136, 2412, 2493, 2610, 3002, 3457
SduI GDGCHC 5 cut(s) 974, 1112, 2368, 2821, 3148
SexAI ACCWGGT 1 cut(s) 2410
SfcI CTRYAG 4 cut(s) 1293, 1797, 2151, 2216
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 2 cut(s) 2914, 3277
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 4 cut(s) 567, 699, 1748, 2004
SmlI CTYRAG 2 cut(s) 32, 1631
SmoI CTYRAG 2 cut(s) 32, 1631
SseBI AGGCCT 1 cut(s) 2983
SspI AATATT 1 cut(s) 2443
SspMI CTAG 7 cut(s) 83, 2373, 2388, 2903, 2985, 3270, 3449
SstI GAGCTC 2 cut(s) 974, 1112
StuI AGGCCT 1 cut(s) 2983
StyD4I CCNGG 7 cut(s) 1710, 2134, 2410, 2491, 2608, 3000, 3455
StyI CCWWGG 6 cut(s) 45, 1448, 2917, 2984, 3101, 3262
TaiI ACGT 3 cut(s) 1780, 2059, 3574
TaqI TCGA 5 cut(s) 33, 1938, 2112, 2823, 3130
TaqII GACCGA 2 cut(s) 408, 606
TatI WGTACW 5 cut(s) 278, 1766, 1899, 1956, 2805
TauI GCSGC 3 cut(s) 829, 1093, 1790
TfiI GAWTC 6 cut(s) 1324, 1421, 1441, 2221, 2537, 2956
Tru1I TTAA 9 cut(s) 306, 879, 1097, 1248, 1320, 1340, 1374, 2228, 2702
Tru9I TTAA 9 cut(s) 306, 879, 1097, 1248, 1320, 1340, 1374, 2228, 2702
TseFI GTSAC 5 cut(s) 776, 1027, 1302, 2065, 2295
TseI GCWGC 6 cut(s) 5, 1349, 1352, 1399, 1402, 2173
Tsp45I GTSAC 5 cut(s) 776, 1027, 1302, 2065, 2295
TspGWI ACGGA 1 cut(s) 694
Van91I CCANNNNNTGG 2 cut(s) 1472, 3382
VpaK11BI GGWCC 2 cut(s) 2914, 3277
XapI RAATTY 4 cut(s) 152, 1820, 2679, 3217
XceI RCATGY 1 cut(s) 1531
XcmI CCANNNNNNNNNTGG 3 cut(s) 86, 2722, 3439
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 2984
XmnI GAANNNNTTC 1 cut(s) 1644
XspI CTAG 7 cut(s) 83, 2373, 2388, 2903, 2985, 3270, 3449
ZrmI AGTACT 2 cut(s) 280, 1958
Zsp2I ATGCAT 1 cut(s) 2200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.