MD17G1229800.v1.1

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000671
Physical Location & Seq
Forward (+)
27784615 .. 27789349
4735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1229800.v1.1.491

Sequence Viewer

Length: 3648 bp
ATGGCGGCTGCCGACGACGGTCTGAAGAAGTTGGAGTACCTTTCGCTGGTCTCCAAAGTCTGTTCAGAGCTCGAGACGCATCTAGGGTTTGGGGACAAAGTTCTGGCGGAGTTCATCACCGAAATCGGACGGAGCTGTGAGACGGTGGACGAATTCGACGCCCGATTGAAGGAGAACGGCGCAGAGATGCCCGACTACTTCGTCCGTACACTCCTCACTATTATACACGCAATCCTCCCTCCGAAGCCGAAGCCGGAGAAGGACGCCTCGAAGAAAGACGGCGCTTCTGAAGGTAAGAAAACCAAGTTTAAGGCTTTGGCTATTGCTGATAATAGAGATAGAGTTAAGGAGATTGATAAGGAAATTGAGATGGAGACTAGGGAGAAGCACACCGACGGAGAGGAGCGGGACGGAGAGCGTGAAGACGATAGGCGGAGGGGTAGGGATAAATATAGGGATGGAGATAGAGATAGAGATAGAGATCGGGATCGTGATAGGCACAGAGGTAGGGATAGAGATGGGAGAGGCAGGGGAAGGGATAGGCATGATAGAGACGAGAGGCGTAGAGATTCACATTATAATGATGATGATGGGGAAAGAGGGAGGCGTAGAGATTCACATTATAATGATGATGATGGGGAAAGGGGGAGGCATACAGATCGATACGGTAAGCATAAGAGAGATGGGTATGAAGAGAATGGAATTGAAAGAGAAGATGATGATAGGAGAGGTAATAGGGATAGGCAGAATGGTCCTAGTAAGAACCTTTCCGATGAACCCGAATTGTACCAGGTGTATAAGGGTAGGGTTTCGAGAGTGATGGACACCGGTTGCTTTGTTCAGTTAAATGATTTGAGAGGGAAGGAAGGTTTGGTTCATGTTTCACAGATGGCAACTCGGCGAATTAGTAATGCTAAGGATGTGGTGAAGAGGGATCAGGAAGTTTATGTTAAGGTGATTTCTGTTTCGGGTCAGAAGTTGAGCCTTTCGATGAGGGATGTTGATCAGCATTCTGGGAAGGATTTGCTTCCGTTGAAGAAGAGCTCAGAGGATGACGCTTATAGGACCAACACGTCGGTGTCTAGAGACGGACCTGTGACTAGGACTGGTCTTTCTGGGATTAGGATTGTGGAAGAGGATGATGTTGGTCCATCACGCCGGCCATTGAAGCGAATGAGCTCACCTGAGAAGTGGGAAGCCAAACAGTTAATTGCCTCAGGTGTTTTGGGTGTGACAGAGTACCCAATGTATGATGATGAAGCAGATGGGATGCTATATCAAGAAGAGGGAGCTGAGGAAGAGCTTGAGATTGAGATCAATGAGGATGAGCCGGCCTTTTTGAATGGGCAGACCAGGTATTCTGTGGATATGTCCCCTGTAAAAATCTTTAAGAATCCAGAAGGGTCTTTGAGTCGTGCAGCTGCACTTCAGTCTGCACTCATTAAGGAGCGTAGAGAAGTGCGAGAGCAGCAGCAAAGAACCATGTTGGATTCCATCCCAAAGGATCTGAATCGTCCCTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCCCAGGAGCTTAGAGGTGTTGGTTTGTCTGCATATGATATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAAACCATCAGCTTTGGGCAGAGGTCTAAGCTATCAATTCAGGAACAGAGGCAGAGCTTGCCTATATACAAGCTGAAGAAAGAATTGGTTCAGGCGGTGAATGAGAATCAAGTGCTCGTCGTCATTGGTGAGACTGGTTCTGGTAAGACAACCCAGGTAACACAGTATCTTGCAGAAGCGGGTTACACGACAAAGGGAAAGATTGGATGTACACAGCCCCGTAGGGTTGCTGCAATGTCCGTAGCCAAGAGGGTGGCTGAAGAGTTTGGTTGTCGTTTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCAGATACTGTCATCAAGTACATGACCGATGGTATGCTTCTTAGGGAGATTTTGATTGATGAGAACCTTTCCCAATATTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCACACAGATGTTCTTTTTGGATTACTGAAGAAGCTTCTGATTCGGAGACCAGACCTTCGTTTGATTGTCACCTCTGCTACTTTGGATGCAGAGAAGTTTTCAGGTTATTTCTTCGACTGTAACATATTTACTATCCCTGGGAGAACTTTTCCTGTTGAGATACTCTACACCAAGCAGCCAGAAAGTGACTACCTTGATGCATCTCTAATTACTGTCCTACAAATCCACTTAACAGAACCTGAAGGTGATATCCTTCTCTTCTTGACTGGACAAGAAGAGATTGATTTTGCATGCCAGTCTCTTTATGAGAGGATGAAGGGTCTTGGTAAAAATGTTCCTGAGTTAATTATTCTACCAGTATATAGTGCCCTTCCTAGTGAAATGCAGTCAAGGATATTTGATCCTGCCCCGCCAGGGAAGAGGAAAGTGGTTGTGGCTACTAATATTGCTGAAGCATCACTGACTATTGATGGGATATTTTATGTCATCGATCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTGGATTCGCTTGTCATTACTCCAATTTCACAAGCATCAGCCAAGCAACGAGCTGGGCGTGCTGGACGCACAGGGCCTGGGAAATGTTACCGCCTCTACACTGAGAGTGCATACCGTAATGAGATGTCCCCTACTTCAATTCCAGAAATCCAGAGGATAAATCTTGGGACTACTACACTTACAATGAAAGCCATGGGGATAAATGATCTCCTGTCTTTTGATTTTATGGATCCTCCTTCACCGCAAGCACTCATTTCTGCCATGGAACAACTGTACAGTTTGGGAGCACTGGACGAGGAGGGGCTGCTGACCAAATTGGGTAGGAAGATGGCTGAGTTTCCTCTCGATCCACCGTTATCTAAGATGCTACTCGCTAGTGTAGACCTTGGATGTAGTGATGAGATCTTGACCATCATTGCAATGATTCAGACAGGCAATATCTTTTACAGGCCTAGGGAAAAACAAGCCCAGGCTGATCAGAAGAGAGCTAAGTTTTTCCAGCCAGAGGGAGACCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAATTTCTCAGGTCCGTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCCTTGAGGAGGGCACAGGATGTCAGAAAACAGCTTCTGAGCATCATGGACAAGTATAAATTGGACGTGGTCAGTGCAGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACAGCAGGTTTCTTCTTCCATGGTGCTAGAAAGGACCCACAGGAGGGTTATAGAACCCTAGTCGAGAACCAGCCAGTTTATATTCACCCAAGCAGTGCTCTCTTCCAGAGACAACCAGATTGGGTTATCTACCATGAGCTGGTTATGACTACAAAGGAGTACATGCGCGAGGTGACGGTTGTTGACCCCAAATGGCTAGTAGAACTGGCACCAAGGTTCTTCAAAGTATCAGATCCCACAAAGATGAGTAAGCGCAAGCGTCAGGAACGAATTGAACCCTTATATGACAGATACCATGAACCTAACTCTTGGCGTCTTAGTAAGCGTCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1216

Amino Acids

138.65

Weight (kDa)

6.32

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 261 - 331 1.7e-12 S1 RNA binding domain
DEAD PF00270 569 - 718 7.7e-08 DEAD/DEAH box helicase
AAA_22 PF13401 578 - 702 9.1e-06 AAA domain
Helicase_C PF00271 762 - 892 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 954 - 982 8.4e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 983 - 1042 6e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1100 - 1176 7.4e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 579, 624, 2586
AasI GACNNNNNNGTC 2 cut(s) 200, 1072
Acc36I ACCTGC 1 cut(s) 3308
AccB1I GGYRCC 1 cut(s) 3520
AccB7I CCANNNNNTGG 2 cut(s) 1541, 3451
AccBSI CCGCTC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 2982
AccII CGCG 1 cut(s) 3480
AciI CCGC 9 cut(s) 5, 107, 406, 433, 1721, 1805, 2477, 2692, 2843
AcoI YGGCCR 1 cut(s) 1160
AcsI RAATTY 3 cut(s) 152, 3157, 3286
AcuI CTGAAG 8 cut(s) 44, 309, 1412, 1721, 1905, 2114, 2328, 2538
AcyI GRCGYC 3 cut(s) 159, 264, 3625
AfaI GTAC 8 cut(s) 38, 208, 788, 1241, 1837, 1970, 2876, 3473
AflIII ACRYGT 1 cut(s) 1071
AgeI ACCGGT 1 cut(s) 827
AgsI TTSAA 8 cut(s) 169, 707, 1036, 1168, 1342, 2739, 3535, 3587
AjiI CACGTC 2 cut(s) 1074, 3268
AjuI GAANNNNNNNTTGG 4 cut(s) 854, 886, 1694, 1726
AleI CACNNNNGTG 1 cut(s) 1076
Alw21I GWGCWC 6 cut(s) 72, 1046, 1181, 1743, 2890, 3412
AlwNI CAGNNNCTG 6 cut(s) 1541, 1958, 2306, 2602, 2678, 3238
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 4 cut(s) 1160, 1332, 2675, 3050
ApeKI GCWGC 8 cut(s) 8, 1418, 1421, 1468, 1471, 1856, 2242, 2905
ApoI RAATTY 3 cut(s) 152, 3157, 3286
ArsI GACNNNNNNTTYG 2 cut(s) 2106, 2138
AsiGI ACCGGT 1 cut(s) 827
Asp700I GAANNNNTTC 1 cut(s) 1713
AspA2I CCTAGG 1 cut(s) 3053
AspLEI GCGC 4 cut(s) 182, 284, 3480, 3567
AspS9I GGNCC 8 cut(s) 752, 1065, 1091, 1148, 1949, 2675, 3167, 3346
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 7 cut(s) 752, 1065, 1091, 1148, 1949, 3167, 3346
AvrII CCTAGG 1 cut(s) 3053
AxyI CCTNAGG 1 cut(s) 1216
BaeGI GKGCMC 2 cut(s) 2437, 3217
BamHI GGATCC 1 cut(s) 2830
BanI GGYRCC 1 cut(s) 3520
BanII GRGCYC 3 cut(s) 72, 1046, 1181
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 6 cut(s) 72, 1046, 1181, 1743, 2890, 3412
BbvCI CCTCAGC 1 cut(s) 1293
BbvI GCAGC 7 cut(s) 1408, 1430, 1480, 1483, 1843, 2254, 2892
BceAI ACGGC 2 cut(s) 193, 295
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BclI TGATCA 2 cut(s) 1003, 3076
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 3308
BglII AGATCT 1 cut(s) 3003
BisI GCNGC 9 cut(s) 6, 9, 1419, 1422, 1469, 1472, 1857, 2243, 2906
BlnI CCTAGG 1 cut(s) 3053
BlsI GCNGC 9 cut(s) 7, 10, 1420, 1423, 1470, 1473, 1858, 2244, 2907
Bme18I GGWCC 7 cut(s) 752, 1065, 1091, 1148, 1949, 3167, 3346
BmeT110I CYCGRG 1 cut(s) 71
BmgBI CACGTC 2 cut(s) 1074, 3268
BmgT120I GGNCC 8 cut(s) 752, 1065, 1091, 1148, 1949, 2675, 3167, 3346
BmiI GGNNCC 3 cut(s) 2832, 3348, 3522
BoxI GACNNNNGTC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 915, 1293
BpuEI CTTGAG 2 cut(s) 1325, 3226
Bsa29I ATCGAT 2 cut(s) 661, 2556
BsaBI GATNNNNATC 5 cut(s) 480, 486, 1002, 1313, 1509
BsaHI GRCGYC 3 cut(s) 159, 264, 3625
BsaI GGTCTC 3 cut(s) 55, 2107, 3105
BsaWI WCCGGW 1 cut(s) 827
Bse118I RCCGGY 3 cut(s) 827, 1158, 1330
Bse21I CCTNAGG 1 cut(s) 1216
Bse3DI GCAATG 3 cut(s) 1866, 3015, 3027
Bse8I GATNNNNATC 5 cut(s) 480, 486, 1002, 1313, 1509
BseCI ATCGAT 2 cut(s) 661, 2556
BseJI GATNNNNATC 5 cut(s) 480, 486, 1002, 1313, 1509
BseMI GCAATG 3 cut(s) 1866, 3015, 3027
BseRI GAGGAG 4 cut(s) 203, 416, 2912, 3223
BseSI GKGCMC 2 cut(s) 2437, 3217
BseXI GCAGC 7 cut(s) 1408, 1430, 1480, 1483, 1843, 2254, 2892
BseYI CCCAGC 1 cut(s) 2654
BsgI GTGCAG 4 cut(s) 1407, 1419, 1437, 3297
Bsh1236I CGCG 1 cut(s) 3480
BshFI GGCC 4 cut(s) 1162, 1334, 2677, 3052
BshNI GGYRCC 1 cut(s) 3520
BshTI ACCGGT 1 cut(s) 827
BshVI ATCGAT 2 cut(s) 661, 2556
BsiHKAI GWGCWC 6 cut(s) 72, 1046, 1181, 1743, 2890, 3412
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 4 cut(s) 254, 828, 1159, 1331
BslFI GGGAC 6 cut(s) 107, 422, 1357, 1500, 2713, 2782
BsmBI CGTCTC 4 cut(s) 68, 134, 546, 1080
BsmFI GGGAC 6 cut(s) 107, 422, 1357, 1500, 2713, 2782
BsmI GAATGC 1 cut(s) 1009
BsnI GGCC 4 cut(s) 1162, 1334, 2677, 3052
Bso31I GGTCTC 3 cut(s) 55, 2107, 3105
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 8 cut(s) 72, 1046, 1181, 1743, 2437, 2890, 3217, 3412
Bsp1407I TGTACA 2 cut(s) 1835, 2874
Bsp19I CCATGG 3 cut(s) 2793, 2862, 3331
BspACI CCGC 9 cut(s) 5, 107, 406, 433, 1721, 1805, 2477, 2692, 2843
BspANI GGCC 4 cut(s) 1162, 1334, 2677, 3052
BspDI ATCGAT 2 cut(s) 661, 2556
BspFNI CGCG 1 cut(s) 3480
BspHI TCATGA 1 cut(s) 2053
BspLI GGNNCC 3 cut(s) 2832, 3348, 3522
BspMI ACCTGC 1 cut(s) 3308
BspQI GCTCTTC 2 cut(s) 1034, 1293
BspT107I GGYRCC 1 cut(s) 3520
BspTNI GGTCTC 3 cut(s) 55, 2107, 3105
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 3 cut(s) 1866, 3015, 3027
BsrFI RCCGGY 3 cut(s) 827, 1158, 1330
BsrGI TGTACA 2 cut(s) 1835, 2874
BssAI RCCGGY 3 cut(s) 827, 1158, 1330
BssNI GRCGYC 3 cut(s) 159, 264, 3625
BssT1I CCWWGG 6 cut(s) 2793, 2862, 2986, 3053, 3331, 3524
BstACI GRCGYC 3 cut(s) 159, 264, 3625
BstAPI GCANNNNNTGC 1 cut(s) 1684
BstAUI TGTACA 2 cut(s) 1835, 2874
BstC8I GCNNGC 7 cut(s) 1160, 1332, 1685, 2359, 2661, 2847, 3569
BstDSI CCRYGG 4 cut(s) 2793, 2862, 3170, 3331
BstENI CCTNNNNNAGG 1 cut(s) 3208
BstFNI CGCG 1 cut(s) 3480
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 4 cut(s) 182, 284, 3480, 3567
BstMWI GCNNNNNNNGC 8 cut(s) 76, 1168, 1468, 1684, 2608, 2660, 3149, 3314
BstNSI RCATGY 2 cut(s) 2361, 3478
BstPAI GACNNNNGTC 1 cut(s) 18
BstSLI GKGCMC 2 cut(s) 2437, 3217
BstUI CGCG 1 cut(s) 3480
BstV1I GCAGC 7 cut(s) 1408, 1430, 1480, 1483, 1843, 2254, 2892
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 5 cut(s) 1504, 1525, 2830, 3003, 3544
BstXI CCANNNNNNTGG 1 cut(s) 1879
BstYI RGATCY 5 cut(s) 1504, 1525, 2830, 3003, 3544
Bsu15I ATCGAT 2 cut(s) 661, 2556
Bsu36I CCTNAGG 1 cut(s) 1216
BsuRI GGCC 4 cut(s) 1162, 1334, 2677, 3052
BsuTUI ATCGAT 2 cut(s) 661, 2556
BtgI CCRYGG 4 cut(s) 2793, 2862, 3170, 3331
BtrI CACGTC 2 cut(s) 1074, 3268
BtsI GCAGTG 1 cut(s) 3412
BtsIMutI CAGTG 6 cut(s) 1944, 2525, 2700, 2888, 3280, 3412
BveI ACCTGC 1 cut(s) 3308
Cac8I GCNNGC 7 cut(s) 1160, 1332, 1685, 2359, 2661, 2847, 3569
CaiI CAGNNNCTG 6 cut(s) 1541, 1958, 2306, 2602, 2678, 3238
CciI TCATGA 1 cut(s) 2053
CfoI GCGC 4 cut(s) 182, 284, 3480, 3567
Cfr10I RCCGGY 3 cut(s) 827, 1158, 1330
Cfr13I GGNCC 8 cut(s) 752, 1065, 1091, 1148, 1949, 2675, 3167, 3346
ClaI ATCGAT 2 cut(s) 661, 2556
CseI GACGC 8 cut(s) 85, 167, 272, 1064, 2676, 3560, 3614, 3626
CsiI ACCWGGT 2 cut(s) 789, 1352
Csp6I GTAC 8 cut(s) 37, 207, 787, 1240, 1836, 1969, 2875, 3472
CspAI ACCGGT 1 cut(s) 827
CviQI GTAC 8 cut(s) 37, 207, 787, 1240, 1836, 1969, 2875, 3472
DrdI GACNNNNNNGTC 2 cut(s) 200, 1072
DseDI GACNNNNNNGTC 2 cut(s) 200, 1072
EaeI YGGCCR 1 cut(s) 1160
EciI GGCGGA 2 cut(s) 122, 448
Ecl136II GAGCTC 3 cut(s) 70, 1044, 1179
Eco130I CCWWGG 6 cut(s) 2793, 2862, 2986, 3053, 3331, 3524
Eco147I AGGCCT 1 cut(s) 3052
Eco24I GRGCYC 3 cut(s) 72, 1046, 1181
Eco31I GGTCTC 3 cut(s) 55, 2107, 3105
Eco32I GATATC 1 cut(s) 2317
Eco47I GGWCC 7 cut(s) 752, 1065, 1091, 1148, 1949, 3167, 3346
Eco53kI GAGCTC 3 cut(s) 70, 1044, 1179
Eco57I CTGAAG 8 cut(s) 44, 309, 1412, 1721, 1905, 2114, 2328, 2538
Eco81I CCTNAGG 1 cut(s) 1216
Eco88I CYCGRG 1 cut(s) 71
EcoICRI GAGCTC 3 cut(s) 70, 1044, 1179
EcoNI CCTNNNNNAGG 1 cut(s) 3208
EcoO109I RGGNCCY 2 cut(s) 2675, 3346
EcoRI GAATTC 1 cut(s) 152
EcoRV GATATC 1 cut(s) 2317
EcoT14I CCWWGG 6 cut(s) 2793, 2862, 2986, 3053, 3331, 3524
EcoT22I ATGCAT 1 cut(s) 2269
EcoT38I GRGCYC 3 cut(s) 72, 1046, 1181
ErhI CCWWGG 6 cut(s) 2793, 2862, 2986, 3053, 3331, 3524
Esp3I CGTCTC 4 cut(s) 68, 134, 546, 1080
FaqI GGGAC 6 cut(s) 107, 422, 1357, 1500, 2713, 2782
FauI CCCGC 3 cut(s) 399, 1798, 2484
FauNDI CATATG 1 cut(s) 1591
FbaI TGATCA 2 cut(s) 1003, 3076
FblI GTMKAC 1 cut(s) 2982
Fnu4HI GCNGC 9 cut(s) 6, 9, 1419, 1422, 1469, 1472, 1857, 2243, 2906
FriOI GRGCYC 3 cut(s) 72, 1046, 1181
Fsp4HI GCNGC 9 cut(s) 6, 9, 1419, 1422, 1469, 1472, 1857, 2243, 2906
GlaI GCGC 4 cut(s) 181, 283, 3479, 3566
GluI GCNGC 9 cut(s) 6, 9, 1419, 1422, 1469, 1472, 1857, 2243, 2906
GsaI CCCAGC 1 cut(s) 2658
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 4 cut(s) 1162, 1334, 2677, 3052
HapII CCGG 4 cut(s) 254, 828, 1159, 1331
HgaI GACGC 8 cut(s) 85, 167, 272, 1064, 2676, 3560, 3614, 3626
HhaI GCGC 4 cut(s) 182, 284, 3480, 3567
Hin1I GRCGYC 3 cut(s) 159, 264, 3625
Hin6I GCGC 4 cut(s) 180, 282, 3478, 3565
HinP1I GCGC 4 cut(s) 180, 282, 3478, 3565
HincII GTYRAC 1 cut(s) 3496
HindII GTYRAC 1 cut(s) 3496
HindIII AAGCTT 1 cut(s) 2099
HpaII CCGG 4 cut(s) 254, 828, 1159, 1331
Hpy166II GTNNAC 5 cut(s) 148, 209, 1838, 2983, 3496
Hpy8I GTNNAC 5 cut(s) 148, 209, 1838, 2983, 3496
Hpy99I CGWCG 7 cut(s) 17, 20, 161, 398, 1078, 1748, 3642
HpyCH4IV ACGT 2 cut(s) 1073, 3267
HpyF10VI GCNNNNNNNGC 8 cut(s) 76, 1168, 1468, 1684, 2608, 2660, 3149, 3314
HpySE526I ACGT 2 cut(s) 1073, 3267
Hsp92I GRCGYC 3 cut(s) 159, 264, 3625
HspAI GCGC 4 cut(s) 180, 282, 3478, 3565
KroI GCCGGC 2 cut(s) 1158, 1330
KroNI GCCGGC 2 cut(s) 1160, 1332
Ksp22I TGATCA 2 cut(s) 1003, 3076
LguI GCTCTTC 2 cut(s) 1034, 1293
LmnI GCTCC 6 cut(s) 132, 403, 1289, 1447, 1564, 2885
Lsp1109I GCAGC 7 cut(s) 1408, 1430, 1480, 1483, 1843, 2254, 2892
MabI ACCWGGT 2 cut(s) 789, 1352
MaeII ACGT 2 cut(s) 1073, 3267
MaeIII GTNAC 9 cut(s) 1096, 1231, 1783, 1808, 2134, 2186, 2252, 2687, 3484
MbiI CCGCTC 1 cut(s) 406
MflI RGATCY 5 cut(s) 1504, 1525, 2830, 3003, 3544
MhlI GDGCHC 8 cut(s) 72, 1046, 1181, 1743, 2437, 2890, 3217, 3412
MlyI GAGTC 1 cut(s) 1420
MmeI TCCRAC 3 cut(s) 12, 1467, 1897
Mph1103I ATGCAT 1 cut(s) 2269
MroNI GCCGGC 2 cut(s) 1158, 1330
MroXI GAANNNNTTC 1 cut(s) 1713
MseI TTAA 9 cut(s) 309, 345, 845, 951, 1208, 1389, 1443, 2297, 2411
MslI CAYNNNNRTG 6 cut(s) 579, 624, 1076, 2073, 3020, 3554
MspA1I CMGCKG 1 cut(s) 1421
MspI CCGG 4 cut(s) 254, 828, 1159, 1331
Mva1269I GAATGC 1 cut(s) 1009
MvnI CGCG 1 cut(s) 3480
MwoI GCNNNNNNNGC 8 cut(s) 76, 1168, 1468, 1684, 2608, 2660, 3149, 3314
NaeI GCCGGC 2 cut(s) 1160, 1332
NcoI CCATGG 3 cut(s) 2793, 2862, 3331
NdeI CATATG 1 cut(s) 1591
NgoMIV GCCGGC 2 cut(s) 1158, 1330
NlaIV GGNNCC 3 cut(s) 2832, 3348, 3522
NmeAIII GCCGAG 1 cut(s) 877
NmuCI GTSAC 5 cut(s) 1096, 1231, 2134, 2252, 3484
NsiI ATGCAT 1 cut(s) 2269
NspI RCATGY 2 cut(s) 2361, 3478
OliI CACNNNNGTG 1 cut(s) 1076
PaeI GCATGC 1 cut(s) 2361
PaeR7I CTCGAG 1 cut(s) 71
PagI TCATGA 1 cut(s) 2053
PasI CCCWGGG 2 cut(s) 1518, 2204
PceI AGGCCT 1 cut(s) 3052
PciSI GCTCTTC 2 cut(s) 1034, 1293
PcsI WCGNNNNNNNCGW 1 cut(s) 2656
PctI GAATGC 1 cut(s) 1009
PdiI GCCGGC 2 cut(s) 1160, 1332
PdmI GAANNNNTTC 1 cut(s) 1713
PfeI GAWTC 9 cut(s) 569, 614, 1393, 1490, 1510, 1732, 2107, 2606, 3025
PflFI GACNNNGTC 1 cut(s) 3269
PflMI CCANNNNNTGG 2 cut(s) 1541, 3451
PfoI TCCNGGA 1 cut(s) 2560
PinAI ACCGGT 1 cut(s) 827
PkrI GCNGC 9 cut(s) 7, 10, 1420, 1423, 1470, 1473, 1858, 2244, 2907
PleI GAGTC 1 cut(s) 1419
PpsI GAGTC 1 cut(s) 1419
PpuMI RGGWCCY 1 cut(s) 3346
PshAI GACNNNNGTC 1 cut(s) 18
PsiI TTATAA 3 cut(s) 579, 624, 2586
Psp124BI GAGCTC 3 cut(s) 72, 1046, 1181
Psp5II RGGWCCY 1 cut(s) 3346
PspFI CCCAGC 1 cut(s) 2654
PspN4I GGNNCC 3 cut(s) 2832, 3348, 3522
PspPI GGNCC 8 cut(s) 752, 1065, 1091, 1148, 1949, 2675, 3167, 3346
PspPPI RGGWCCY 1 cut(s) 3346
PstNI CAGNNNCTG 6 cut(s) 1541, 1958, 2306, 2602, 2678, 3238
PsuI RGATCY 5 cut(s) 1504, 1525, 2830, 3003, 3544
PsyI GACNNNGTC 1 cut(s) 3269
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 8 cut(s) 38, 208, 788, 1241, 1837, 1970, 2876, 3473
RsaNI GTAC 8 cut(s) 37, 207, 787, 1240, 1836, 1969, 2875, 3472
RseI CAYNNNNRTG 6 cut(s) 579, 624, 1076, 2073, 3020, 3554
SacI GAGCTC 3 cut(s) 72, 1046, 1181
SapI GCTCTTC 2 cut(s) 1034, 1293
SaqAI TTAA 9 cut(s) 309, 345, 845, 951, 1208, 1389, 1443, 2297, 2411
SatI GCNGC 9 cut(s) 6, 9, 1419, 1422, 1469, 1472, 1857, 2243, 2906
Sau96I GGNCC 8 cut(s) 752, 1065, 1091, 1148, 1949, 2675, 3167, 3346
SchI GAGTC 1 cut(s) 1420
SduI GDGCHC 8 cut(s) 72, 1046, 1181, 1743, 2437, 2890, 3217, 3412
SexAI ACCWGGT 2 cut(s) 789, 1352
Sfr274I CTCGAG 1 cut(s) 71
SinI GGWCC 7 cut(s) 752, 1065, 1091, 1148, 1949, 3167, 3346
SlaI CTCGAG 1 cut(s) 71
SmiMI CAYNNNNRTG 6 cut(s) 579, 624, 1076, 2073, 3020, 3554
SmlI CTYRAG 3 cut(s) 71, 1304, 3205
SmoI CTYRAG 3 cut(s) 71, 1304, 3205
SphI GCATGC 1 cut(s) 2361
SseBI AGGCCT 1 cut(s) 3052
SsiI CCGC 9 cut(s) 5, 107, 406, 433, 1721, 1805, 2477, 2692, 2843
SspI AATATT 2 cut(s) 2027, 2512
SstI GAGCTC 3 cut(s) 72, 1046, 1181
StuI AGGCCT 1 cut(s) 3052
StyI CCWWGG 6 cut(s) 2793, 2862, 2986, 3053, 3331, 3524
TaiI ACGT 2 cut(s) 1076, 3270
TaqII GACCGA 1 cut(s) 1991
TatI WGTACW 4 cut(s) 1835, 1968, 2874, 3471
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 9 cut(s) 569, 614, 1393, 1490, 1510, 1732, 2107, 2606, 3025
Tru1I TTAA 9 cut(s) 309, 345, 845, 951, 1208, 1389, 1443, 2297, 2411
Tru9I TTAA 9 cut(s) 309, 345, 845, 951, 1208, 1389, 1443, 2297, 2411
TscAI CASTG 6 cut(s) 1951, 2532, 2707, 2895, 3280, 3412
TseFI GTSAC 5 cut(s) 1096, 1231, 2134, 2252, 3484
TseI GCWGC 8 cut(s) 8, 1418, 1421, 1468, 1471, 1856, 2242, 2905
Tsp45I GTSAC 5 cut(s) 1096, 1231, 2134, 2252, 3484
TspDTI ATGAA 9 cut(s) 103, 705, 789, 866, 1272, 2061, 2396, 2801, 3624
TspGWI ACGGA 8 cut(s) 145, 194, 411, 426, 1020, 1104, 1855, 3159
TspRI CASTG 6 cut(s) 1951, 2532, 2707, 2895, 3280, 3412
Tth111I GACNNNGTC 1 cut(s) 3269
Van91I CCANNNNNTGG 2 cut(s) 1541, 3451
VpaK11BI GGWCC 7 cut(s) 752, 1065, 1091, 1148, 1949, 3167, 3346
XagI CCTNNNNNAGG 1 cut(s) 3208
XapI RAATTY 3 cut(s) 152, 3157, 3286
XbaI TCTAGA 1 cut(s) 1082
XceI RCATGY 2 cut(s) 2361, 3478
XcmI CCANNNNNNNNNTGG 1 cut(s) 1360
XhoI CTCGAG 1 cut(s) 71
XmaJI CCTAGG 1 cut(s) 3053
XmiI GTMKAC 1 cut(s) 2982
XmnI GAANNNNTTC 1 cut(s) 1713
Zsp2I ATGCAT 1 cut(s) 2269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.