RchiOBHm_Chr1g0351781

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000671
Physical Location & Seq
Forward (+)
45011144 .. 45018424
7281 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57753

Sequence Viewer

Length: 3615 bp
ATGGCTGTGATTGATGATGGTTTGAAGAAGCTCGAGTACCTTTCCTTGGTTTCCAAGGTCTGTTCAGAGCTGGAAACTCATCTAGGGTTTGGGGACAAAGTTCTAGCCGAGTTCATAACTGAGATGGGACGAAACTGTGAGAGTGTGGATGAATTTGATGCCAAATTGAAGGAACACGGTGCCGAGATGCCTGATTACTTTGTCCGTACACTCCTCACTATCATTCACGCAATTCTTCCTCCCAAGGCAAAGTCCGAGAACGACTCAAAGAAAGAGAGCACTGCTGAAGGTAGTAAAAGCAAGTTCAAGGCTTTGTCTATTGCTGATAACAGGGATAGGGTAAAGGATATCCAGAGGGAAATTGAGTTGGAGGCCAAGGAAAAGCGAAATGACAGAGAAGAATGGGGAGATGGGCGTGAAGAAGATAGGCCCAAAGGTAGAGATGGAGGTAGAGGTAGGGATAGGGAAAGAGATACAAATCGAGACAGAGACAGGGACAGGGACAGGGACAGGGACAGGGATAGGGATAGGAGGGATAGGAGAGAGAGGGGAAGGGATAGGTATGATAGAGATGAAAGGCGGAGAGATAATCATTATCATGATTATGATAAGGGAAGGCATACAGATCGATCCAATAAGCACAGGAGGGATGAGTATGAAAAGGACGGAGATGTTAGAGAAGATGATGTTGATAGGAGAGGAAGTAGGGATCAGCGGAATGGATTGCACCATTCTGATGAGCCTGAATTGTATAAGGTTTATAAGGGTAGGGTCTCAAAAGTGATGGACACAGGTTGCTTTGTTCAGTTCAGCGATTTTAGAGGGAAGGAGGGTTTGGTTCATGTTTCGCAGATTGCTACTCGGCGAATTGGTAATGCTAAAGATGTGGTGAAGAGAGATCAAGAGGTCTATGTTAAGGTGATTTCAATTTCTGGTCAAAAGTTGAGCCTGTCAATGAGGGATGTTGATCAACATACTGGGAAGGATTTGCTACCTTTGAAGAAGAACTCAGAGGATGATTCTCTTAGGACAAACCCATCGATATCAAAGGATGATGGGCCTGTGACAAGGACGGGTCTCTCTGGGATTAGGATTGTGGAAGAGGATGTTACTGCCCCATCTCGCCGGCCATTGAAGAGAATGAGCTCACCGGAGAAGTGGGAAGCCAAACAGTTGATTGCCTCGGGTGTTTTGGGTGTCAAAGAGTATCCAATGTATGATGAAGAAACAGATGGGATGCTTTATGAAGAAGAGGGAGCTGAGGAAGAACTTGAGATTGAGCTTAATGAGGATGAGCCAGCCTTCTTGCAAGGGCAAACCAGGTATTCTGCAGATATGTCACCTGTGAAAATATTTAAGAATCCAGAAGGGTCATTGAGTCGTGCAGCTGCACTTCAATCTGCACTCATTAAGGAGCGTAGAGAAGTGCGTGAGCAGCAGCAAAGAACCATGTTGGATTCAATCCCAAAGGATCTGAATCGTCCTTGGGAAGATCCAATGCCAGAGACTGGTGAGAGGCATCTTGCACAGGAACTTAGAGGTGTTGGTTTGTCGGCCTATGACATGCCTGAGTGGAAGAAGGATGCTTTTGGGAAGACCGTCACTTTTGGGCAGAGGTCAAAGCTTTCAATTCAGGAACAGAGGCAGAGCTTGCCTATCTATAAGCTGAAGAAAGAATTGGTTCAGGCAGTGCATGAAAATCAAGTTCTTGTTGTCATTGGTGAGACTGGTTCAGGCAAGACAACCCAGGTAACGCAGTATCTTGCAGAAGCTGGGTACACGACAATGGGTAAGATTGGGTGTACACAGCCACGTAGGGTGGCTGCAATGTCTGTAGCCAAGAGGGTTGCTGAAGAGTTTGGTTGTCGTCTGGGGGAGGAAGTTGGATATGCTATTCGTTTTGAGGATTGCACTGGACCTGATACTGTAATCAAGTACATGACGGATGGTATGCTTCTTAGGGAGATTCTACTTGACGAGAACCTTTCTCAGTACTCTGTGGTCATGCTTGATGAAGCTCATGAGAGGACAATCCATACAGATGTTCTTTTTGGATTATTGAAGAAACTTGTGAAGCGGAGACCAGACCTTCGTTTGATTGTCACATCTGCTACCTTGGATGCTGAGAAGTTTTCAGGTTATTTCTTCGATTGTAACATCTTCACTATCCCCGGAAGAACTTTTCCTGTAGAGATACTCTACACCAAGCAGCCAGAAAGTGATTATCTTGATGCATCTTTAATCACTGTTCTACAAATTCACTTAACGGAGCCAGAAGGTGACATCCTTCTCTTTTTGACGGGACAGGAAGAAATTGATTTTGCATGCCAGTCACTATATGAGAGGATGAAGGGTCTTGGTAAAAATGTGCCTGAGTTGATTATCTTACCAGTTTATAGTGCCCTTCCCAGTGAAATGCAGTCGAGGATATTTGATCCAGCCCCACCTGGGAAGAGGAAAGTAGTTGTGGCTACCAATATAGCCGAGGCATCTCTGACCATCGATGGGATATTTTATGTAATTGACCCTGGATTTGCAAAGCAAAATGTTTATAACCCAAAGCAGGGGCTAGATTCACTAGTCATAACTCCAATATCACAAGCATCGGCCAAACAACGAGCTGGGCGTGCTGGCCGTACAGGGCCTGGGAAATGTTACCGCCTTTACACTGAGAGTGCATACCGCAATGAGATGTCCCCTACTTCAGTTCCAGAAATTCAGAGGATAAATCTTGGGACTACTACATTAACGATGAAAGCAATGGGGATAAATGATCTCTTGTCTTTTGATTTTATGGATCCTCCTTCACCCCAAGCACTCATTTCTGCTATGGAACAACTATACAGTTTAGGAGCCCTAGATGAAGAGGGTCTTCTGACCAAATTGGGTAGGAAAATGGCCGAGTTTCCTCTGGATCCACCATTATCTAAGATGCTACTGGCCAGCGTGGACCTTGGATGCAGTGATGAGATCCTGACTATCATTGCCATGATTCAGACAGGCAATATATTTTACAGGCCTAGGGAAAAGCAAGCCCAGGCTGATCAGAAGAGAGCCAAGTTTTTCCAGCCAGAGGGAGATCATCTGACTTTACTTGCAGTCTATGAGGCTTGGAAAGCTAAGAACTTTTCAGGGCCTTGGTGTTTTGAGAACTTTGTTCAGTCTCGATCTCTTAGGAGGGCGCAGGATGTCAGAAAACAGCTTCTAAGCATCATGGATAAGTATAAACTGGACGTTGTGAGTGCTGGAAAGAATTTTACAAAGATCAGGAAGGCAATTACTGCAGGCTTTTTCTTCCATGCTGCTAGAAAGGACCCACAGGAGGGGTATAGAACCCTAGTGGAGAACCAGCCAGTTTATATCCATCCAAGCAGTGCTCTCTTTCAGAGACAACCGGATTGGGTCATCTACCATGAGTTGGTTATGACTACAAAGGAGTATATGCGGGAGGTGACAGTTGTTGACCCCAAATGGCTTGTGGAACTAGCACCCAGGTTCTTCACGGTGGCTGATCCTACCAAGATGAGTAAGCGCAAGCGTCAAGAACGTATTGAACCGCTATATGACAGATACCATGAACCAAACTCCTGGCGTCTTAGTAAACGGCGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

1204

Amino Acids

137.78

Weight (kDa)

6.48

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 252 - 319 4.3e-11 S1 RNA binding domain
DEAD PF00270 558 - 707 1e-07 DEAD/DEAH box helicase
AAA_22 PF13401 566 - 699 1.6e-06 AAA domain
Helicase_C PF00271 751 - 881 1.1e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 943 - 971 8.3e-11 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 972 - 1031 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1089 - 1165 1e-26 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000671)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26560
fragaria_vesca FvH4_3g44820 FvH4_4g07500 FvH4_4g07500 FvH4_4g07500 FvH4_4g07850 FvH4_4g07850 FvH4_7g12750
malus_domestica MD09G1231900.v1.1 MD15G1068900.v1.1 MD15G1069000.v1.1 MD16G1254900.v1.1 MD17G1229800.v1.1
prunus_persica Prupe.1G082300_v2.0.a1 Prupe.1G368500_v2.0.a1 Prupe.1G422500_v2.0.a1 Prupe.2G077500_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G077900_v2.0.a1 Prupe.2G078200_v2.0.a1
pyrus_communis pycom08g06730 pycom09g15120 pycom15g06530 pycom16g21640 pycom17g23220 pycom17g23230 pycom17g23240
rosa_chinensis RchiOBHm_Chr1g0351781 RchiOBHm_Chr4g0400041 RchiOBHm_Chr4g0401121 RchiOBHm_Chr5g0079971 RchiOBHm_Chr5g0081521 RchiOBHm_Chr7g0219091
rosa_laevigata RLG00000009162 RLG00000009217 RLG00000028384 RLG00000036944
rosa_multiflora Rmu_co8434143.1_g000001 Rmu_sc0001909.1_g000017 Rmu_sc0001909.1_g000025 Rmu_sc0001933.1_g000012 Rmu_sc0004005.1_g000001 Rmu_sc0007702.1_g000002 Rmu_sc0010608.1_g000004
rosa_roxburghii Rroxscaffold_1G00002030 Rroxscaffold_4G00303550 Rroxscaffold_5G00345000 Rroxscaffold_5G00345950 Rroxscaffold_5G00345970
rosa_rugosa Rorug01G0221400 Rorug01G0221500 Rorug04G0022300 Rorug04G0022400 Rorug04G0028000 Rorug05G0476900
rosa_samantha Rh1AG235400 Rh1BG205800 Rh1CG220000 Rh1DG232900 Rh2AG475800 Rh2BG083000 Rh2CG085000 Rh4AG097800 Rh4AG105600 Rh4AG109800 Rh4BG095800 Rh4CG107200 Rh4CG114200 Rh4DG092600 Rh4DG097800 Rh5BG553100 Rh5DG563200
rosa_wichuraiana Rw1G020490 Rw4G008160 Rw4G008530 Rw5G049150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 762, 2553
AccB1I GGYRCC 1 cut(s) 179
AccB7I CCANNNNNTGG 2 cut(s) 1508, 3418
AciI CCGC 7 cut(s) 580, 715, 2077, 2659, 2683, 3445, 3557
AcoI YGGCCR 5 cut(s) 1127, 2607, 2632, 2898, 2940
AcsI RAATTY 4 cut(s) 152, 2256, 2715, 3253
AcuI CTGAAG 4 cut(s) 306, 1688, 1872, 2688
AcyI GRCGYC 1 cut(s) 3592
AfaI GTAC 7 cut(s) 38, 208, 1778, 1804, 1937, 1994, 2638
AhlI ACTAGT 1 cut(s) 2578
AjnI CCWGG 8 cut(s) 1319, 1746, 2446, 2527, 2644, 3036, 3491, 3587
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 1661, 1693
Alw21I GWGCWC 3 cut(s) 281, 1148, 3379
Alw26I GTCTC 9 cut(s) 477, 483, 778, 1082, 1499, 1718, 2074, 3168, 3382
AlwNI CAGNNNCTG 3 cut(s) 1508, 1772, 2645
Ama87I CYCGRG 2 cut(s) 32, 1183
ApeKI GCWGC 7 cut(s) 1385, 1388, 1435, 1438, 1823, 2209, 3302
ApoI RAATTY 4 cut(s) 152, 2256, 2715, 3253
ArsI GACNNNNNNTTYG 4 cut(s) 2073, 2105, 2516, 2548
Asp700I GAANNNNTTC 1 cut(s) 1680
AspA2I CCTAGG 1 cut(s) 3020
AspLEI GCGC 2 cut(s) 3184, 3534
AspS9I GGNCC 7 cut(s) 429, 1058, 1916, 2642, 2950, 3134, 3313
AsuC2I CCSGG 1 cut(s) 2172
AsuHPI GGTGA 9 cut(s) 901, 931, 1140, 1332, 1523, 1733, 2291, 2799, 3463
AvaI CYCGRG 2 cut(s) 32, 1183
AvaII GGWCC 3 cut(s) 1916, 2950, 3313
AvrII CCTAGG 1 cut(s) 3020
BaeGI GKGCMC 1 cut(s) 2404
BalI TGGCCA 1 cut(s) 2942
BamHI GGATCC 2 cut(s) 2797, 2914
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 1148, 2857
BarI GAAGNNNNNNTAC 2 cut(s) 20, 52
BbsI GAAGAC 2 cut(s) 1601, 2864
Bbv12I GWGCWC 3 cut(s) 281, 1148, 3379
BbvCI CCTCAGC 1 cut(s) 1260
BbvI GCAGC 7 cut(s) 1375, 1397, 1447, 1450, 1810, 2221, 3289
BceAI ACGGC 1 cut(s) 2619
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 8 cut(s) 1321, 1748, 2448, 2529, 2646, 3038, 3493, 3589
BciVI GTATCC 1 cut(s) 1218
BclI TGATCA 2 cut(s) 967, 3043
BcnI CCSGG 1 cut(s) 2172
BcoDI GTCTC 9 cut(s) 477, 483, 778, 1082, 1499, 1718, 2074, 3168, 3382
BcuI ACTAGT 1 cut(s) 2578
BfaI CTAG 9 cut(s) 83, 104, 2570, 2579, 2858, 3021, 3306, 3338, 3485
BfmI CTRYAG 4 cut(s) 1329, 1833, 2187, 3282
BfuI GTATCC 1 cut(s) 1218
BisI GCNGC 7 cut(s) 1386, 1389, 1436, 1439, 1824, 2210, 3303
BlnI CCTAGG 1 cut(s) 3020
BlsI GCNGC 7 cut(s) 1387, 1390, 1437, 1440, 1825, 2211, 3304
BmcAI AGTACT 1 cut(s) 1994
Bme1390I CCNGG 9 cut(s) 1321, 1748, 2172, 2448, 2529, 2646, 3038, 3493, 3589
Bme18I GGWCC 3 cut(s) 1916, 2950, 3313
BmeT110I CYCGRG 2 cut(s) 32, 1183
BmgT120I GGNCC 7 cut(s) 429, 1058, 1916, 2642, 2950, 3134, 3313
BmiI GGNNCC 6 cut(s) 181, 2271, 2799, 2854, 2916, 3315
BmrFI CCNGG 9 cut(s) 1321, 1748, 2172, 2448, 2529, 2646, 3038, 3493, 3589
BmrI ACTGGG 2 cut(s) 987, 2403
BmuI ACTGGG 2 cut(s) 987, 2403
BpiI GAAGAC 2 cut(s) 1601, 2864
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bpu10I CCTNAGC 1 cut(s) 1260
BpuEI CTTGAG 1 cut(s) 1292
BpuMI CCSGG 1 cut(s) 2172
Bsa29I ATCGAT 3 cut(s) 628, 1040, 2502
BsaAI YACGTR 1 cut(s) 1814
BsaBI GATNNNNATC 3 cut(s) 477, 966, 1476
BsaHI GRCGYC 1 cut(s) 3592
BsaI GGTCTC 3 cut(s) 778, 1082, 2074
BsaWI WCCGGW 2 cut(s) 1150, 3394
BsaXI ACNNNNNCTCC 2 cut(s) 688, 718
Bse118I RCCGGY 1 cut(s) 1125
Bse3DI GCAATG 4 cut(s) 1833, 2692, 2766, 2982
Bse8I GATNNNNATC 3 cut(s) 477, 966, 1476
BseBI CCWGG 8 cut(s) 1321, 1748, 2448, 2529, 2646, 3038, 3493, 3589
BseCI ATCGAT 3 cut(s) 628, 1040, 2502
BseJI GATNNNNATC 3 cut(s) 477, 966, 1476
BseMI GCAATG 4 cut(s) 1833, 2692, 2766, 2982
BseMII CTCAG 8 cut(s) 111, 1023, 1251, 1560, 2003, 2115, 2364, 2661
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 2404
BseXI GCAGC 7 cut(s) 1375, 1397, 1447, 1450, 1810, 2221, 3289
BseYI CCCAGC 2 cut(s) 1772, 2621
BsgI GTGCAG 3 cut(s) 1374, 1386, 1404
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 3 cut(s) 628, 1040, 2502
BsiHKAI GWGCWC 3 cut(s) 281, 1148, 3379
BsiHKCI CYCGRG 2 cut(s) 32, 1183
BsiSI CCGG 4 cut(s) 1126, 1151, 2172, 3395
BslFI GGGAC 9 cut(s) 107, 141, 509, 515, 521, 527, 2316, 2680, 2749
BsmAI GTCTC 9 cut(s) 477, 483, 778, 1082, 1499, 1718, 2074, 3168, 3382
BsmFI GGGAC 9 cut(s) 107, 141, 509, 515, 521, 527, 2316, 2680, 2749
Bso31I GGTCTC 3 cut(s) 778, 1082, 2074
BsoBI CYCGRG 2 cut(s) 32, 1183
Bsp1286I GDGCHC 5 cut(s) 281, 1148, 2404, 2857, 3379
Bsp1407I TGTACA 1 cut(s) 1802
BspACI CCGC 7 cut(s) 580, 715, 2077, 2659, 2683, 3445, 3557
BspCNI CTCAG 8 cut(s) 112, 1022, 1252, 1561, 2002, 2116, 2365, 2662
BspDI ATCGAT 3 cut(s) 628, 1040, 2502
BspHI TCATGA 2 cut(s) 598, 2020
BspLI GGNNCC 6 cut(s) 181, 2271, 2799, 2854, 2916, 3315
BspMAI CTGCAG 2 cut(s) 1333, 3286
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 3 cut(s) 778, 1082, 2074
BsrDI GCAATG 4 cut(s) 1833, 2692, 2766, 2982
BsrFI RCCGGY 1 cut(s) 1125
BsrGI TGTACA 1 cut(s) 1802
BssAI RCCGGY 1 cut(s) 1125
BssNI GRCGYC 1 cut(s) 3592
BssT1I CCWWGG 9 cut(s) 45, 54, 243, 375, 1484, 2115, 2953, 3020, 3137
Bst2UI CCWGG 8 cut(s) 1321, 1748, 2448, 2529, 2646, 3038, 3493, 3589
Bst4CI ACNGT 9 cut(s) 137, 179, 1173, 1600, 1927, 2248, 2846, 3457, 3505
Bst6I CTCTTC 8 cut(s) 887, 1095, 1130, 1245, 1848, 2447, 2859, 3044
BstACI GRCGYC 1 cut(s) 3592
BstAPI GCANNNNNTGC 2 cut(s) 1651, 3281
BstAUI TGTACA 1 cut(s) 1802
BstBAI YACGTR 1 cut(s) 1814
BstHHI GCGC 2 cut(s) 3184, 3534
BstMAI GTCTC 9 cut(s) 477, 483, 778, 1082, 1499, 1718, 2074, 3168, 3382
BstMWI GCNNNNNNNGC 5 cut(s) 1435, 1651, 2627, 3116, 3281
BstNI CCWGG 8 cut(s) 1321, 1748, 2448, 2529, 2646, 3038, 3493, 3589
BstNSI RCATGY 2 cut(s) 1567, 2328
BstSCI CCNGG 9 cut(s) 1319, 1746, 2170, 2446, 2527, 2644, 3036, 3491, 3587
BstSFI CTRYAG 4 cut(s) 1329, 1833, 2187, 3282
BstSLI GKGCMC 1 cut(s) 2404
BstV1I GCAGC 7 cut(s) 1375, 1397, 1447, 1450, 1810, 2221, 3289
BstV2I GAAGAC 2 cut(s) 1601, 2864
BstX2I RGATCY 5 cut(s) 1471, 1492, 2797, 2914, 2970
BstXI CCANNNNNNTGG 1 cut(s) 3588
BstYI RGATCY 5 cut(s) 1471, 1492, 2797, 2914, 2970
Bsu15I ATCGAT 3 cut(s) 628, 1040, 2502
BsuI GTATCC 1 cut(s) 1218
BsuTUI ATCGAT 3 cut(s) 628, 1040, 2502
BtsI GCAGTG 4 cut(s) 279, 1695, 2968, 3379
BtsIMutI CAGTG 8 cut(s) 279, 1695, 1911, 2244, 2416, 2667, 2968, 3379
CaiI CAGNNNCTG 3 cut(s) 1508, 1772, 2645
CciI TCATGA 2 cut(s) 598, 2020
CfoI GCGC 2 cut(s) 3184, 3534
Cfr10I RCCGGY 1 cut(s) 1125
Cfr13I GGNCC 7 cut(s) 429, 1058, 1916, 2642, 2950, 3134, 3313
ClaI ATCGAT 3 cut(s) 628, 1040, 2502
CseI GACGC 2 cut(s) 3527, 3581
CsiI ACCWGGT 1 cut(s) 1319
Csp6I GTAC 7 cut(s) 37, 207, 1777, 1803, 1936, 1993, 2637
CviQI GTAC 7 cut(s) 37, 207, 1777, 1803, 1936, 1993, 2637
EaeI YGGCCR 5 cut(s) 1127, 2607, 2632, 2898, 2940
Eam1104I CTCTTC 8 cut(s) 887, 1095, 1130, 1245, 1848, 2447, 2859, 3044
EarI CTCTTC 8 cut(s) 887, 1095, 1130, 1245, 1848, 2447, 2859, 3044
EciI GGCGGA 1 cut(s) 595
Ecl136II GAGCTC 1 cut(s) 1146
Eco130I CCWWGG 9 cut(s) 45, 54, 243, 375, 1484, 2115, 2953, 3020, 3137
Eco147I AGGCCT 1 cut(s) 3019
Eco24I GRGCYC 2 cut(s) 1148, 2857
Eco31I GGTCTC 3 cut(s) 778, 1082, 2074
Eco32I GATATC 2 cut(s) 349, 1044
Eco47I GGWCC 3 cut(s) 1916, 2950, 3313
Eco53kI GAGCTC 1 cut(s) 1146
Eco57I CTGAAG 4 cut(s) 306, 1688, 1872, 2688
Eco88I CYCGRG 2 cut(s) 32, 1183
EcoICRI GAGCTC 1 cut(s) 1146
EcoO109I RGGNCCY 3 cut(s) 2642, 3134, 3313
EcoRII CCWGG 8 cut(s) 1319, 1746, 2446, 2527, 2644, 3036, 3491, 3587
EcoRV GATATC 2 cut(s) 349, 1044
EcoT14I CCWWGG 9 cut(s) 45, 54, 243, 375, 1484, 2115, 2953, 3020, 3137
EcoT22I ATGCAT 1 cut(s) 2236
EcoT38I GRGCYC 2 cut(s) 1148, 2857
ErhI CCWWGG 9 cut(s) 45, 54, 243, 375, 1484, 2115, 2953, 3020, 3137
FalI AAGNNNNNCTT 2 cut(s) 2856, 2888
FaqI GGGAC 9 cut(s) 107, 141, 509, 515, 521, 527, 2316, 2680, 2749
FauI CCCGC 1 cut(s) 3438
FbaI TGATCA 2 cut(s) 967, 3043
Fnu4HI GCNGC 7 cut(s) 1386, 1389, 1436, 1439, 1824, 2210, 3303
FriOI GRGCYC 2 cut(s) 1148, 2857
Fsp4HI GCNGC 7 cut(s) 1386, 1389, 1436, 1439, 1824, 2210, 3303
FspBI CTAG 9 cut(s) 83, 104, 2570, 2579, 2858, 3021, 3306, 3338, 3485
GlaI GCGC 2 cut(s) 3183, 3533
GluI GCNGC 7 cut(s) 1386, 1389, 1436, 1439, 1824, 2210, 3303
GsaI CCCAGC 2 cut(s) 1776, 2625
HapII CCGG 4 cut(s) 1126, 1151, 2172, 3395
HgaI GACGC 2 cut(s) 3527, 3581
HhaI GCGC 2 cut(s) 3184, 3534
Hin1I GRCGYC 1 cut(s) 3592
Hin6I GCGC 2 cut(s) 3182, 3532
HinP1I GCGC 2 cut(s) 3182, 3532
HincII GTYRAC 1 cut(s) 3463
HindII GTYRAC 1 cut(s) 3463
HindIII AAGCTT 1 cut(s) 1622
HinfI GANTC 9 cut(s) 263, 1019, 1360, 1378, 1457, 1477, 1966, 2573, 2992
HpaII CCGG 4 cut(s) 1126, 1151, 2172, 3395
HphI GGTGA 9 cut(s) 901, 931, 1140, 1332, 1523, 1733, 2291, 2799, 3463
Hpy166II GTNNAC 7 cut(s) 209, 1779, 1803, 1805, 2950, 3463, 3602
Hpy8I GTNNAC 7 cut(s) 209, 1779, 1803, 1805, 2950, 3463, 3602
HpyCH4III ACNGT 9 cut(s) 137, 179, 1173, 1600, 1927, 2248, 2846, 3457, 3505
HpyCH4IV ACGT 3 cut(s) 1813, 3234, 3547
HpyF10VI GCNNNNNNNGC 5 cut(s) 1435, 1651, 2627, 3116, 3281
HpySE526I ACGT 3 cut(s) 1813, 3234, 3547
Hsp92I GRCGYC 1 cut(s) 3592
HspAI GCGC 2 cut(s) 3182, 3532
KroI GCCGGC 1 cut(s) 1125
KroNI GCCGGC 1 cut(s) 1127
Ksp22I TGATCA 2 cut(s) 967, 3043
LmnI GCTCC 4 cut(s) 1256, 1414, 2269, 2852
Lsp1109I GCAGC 7 cut(s) 1375, 1397, 1447, 1450, 1810, 2221, 3289
MabI ACCWGGT 1 cut(s) 1319
MaeI CTAG 9 cut(s) 83, 104, 2570, 2579, 2858, 3021, 3306, 3338, 3485
MaeII ACGT 3 cut(s) 1813, 3234, 3547
MflI RGATCY 5 cut(s) 1471, 1492, 2797, 2914, 2970
MhlI GDGCHC 5 cut(s) 281, 1148, 2404, 2857, 3379
MlsI TGGCCA 1 cut(s) 2942
MluNI TGGCCA 1 cut(s) 2942
MlyI GAGTC 2 cut(s) 257, 1387
MmeI TCCRAC 3 cut(s) 348, 1434, 1864
Mox20I TGGCCA 1 cut(s) 2942
Mph1103I ATGCAT 1 cut(s) 2236
MroNI GCCGGC 1 cut(s) 1125
MroXI GAANNNNTTC 1 cut(s) 1680
MscI TGGCCA 1 cut(s) 2942
MseI TTAA 7 cut(s) 915, 1284, 1356, 1410, 2240, 2264, 2747
MslI CAYNNNNRTG 6 cut(s) 597, 603, 735, 1784, 2040, 2987
Msp20I TGGCCA 1 cut(s) 2942
MspA1I CMGCKG 2 cut(s) 715, 1388
MspI CCGG 4 cut(s) 1126, 1151, 2172, 3395
MspR9I CCNGG 9 cut(s) 1321, 1748, 2172, 2448, 2529, 2646, 3038, 3493, 3589
MvaI CCWGG 8 cut(s) 1321, 1748, 2448, 2529, 2646, 3038, 3493, 3589
MwoI GCNNNNNNNGC 5 cut(s) 1435, 1651, 2627, 3116, 3281
NaeI GCCGGC 1 cut(s) 1127
NciI CCSGG 1 cut(s) 2172
NgoMIV GCCGGC 1 cut(s) 1125
NlaIV GGNNCC 6 cut(s) 181, 2271, 2799, 2854, 2916, 3315
NmeAIII GCCGAG 5 cut(s) 133, 208, 841, 2509, 2926
NmuCI GTSAC 7 cut(s) 1063, 1338, 1600, 2101, 2279, 2331, 3451
NsiI ATGCAT 1 cut(s) 2236
NspI RCATGY 2 cut(s) 1567, 2328
PaeI GCATGC 1 cut(s) 2328
PaeR7I CTCGAG 1 cut(s) 32
PagI TCATGA 2 cut(s) 598, 2020
PceI AGGCCT 1 cut(s) 3019
PcsI WCGNNNNNNNCGW 1 cut(s) 2623
PdiI GCCGGC 1 cut(s) 1127
PdmI GAANNNNTTC 1 cut(s) 1680
PfeI GAWTC 7 cut(s) 1019, 1360, 1457, 1477, 1966, 2573, 2992
PflMI CCANNNNNTGG 2 cut(s) 1508, 3418
PkrI GCNGC 7 cut(s) 1387, 1390, 1437, 1440, 1825, 2211, 3304
PleI GAGTC 2 cut(s) 257, 1386
PpsI GAGTC 2 cut(s) 257, 1386
Ppu21I YACGTR 1 cut(s) 1814
PpuMI RGGWCCY 1 cut(s) 3313
PsiI TTATAA 2 cut(s) 762, 2553
Psp124BI GAGCTC 1 cut(s) 1148
Psp5II RGGWCCY 1 cut(s) 3313
Psp6I CCWGG 8 cut(s) 1319, 1746, 2446, 2527, 2644, 3036, 3491, 3587
PspFI CCCAGC 2 cut(s) 1772, 2621
PspGI CCWGG 8 cut(s) 1319, 1746, 2446, 2527, 2644, 3036, 3491, 3587
PspN4I GGNNCC 6 cut(s) 181, 2271, 2799, 2854, 2916, 3315
PspPI GGNCC 7 cut(s) 429, 1058, 1916, 2642, 2950, 3134, 3313
PspPPI RGGWCCY 1 cut(s) 3313
PspXI VCTCGAGB 1 cut(s) 32
PstI CTGCAG 2 cut(s) 1333, 3286
PstNI CAGNNNCTG 3 cut(s) 1508, 1772, 2645
PsuI RGATCY 5 cut(s) 1471, 1492, 2797, 2914, 2970
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 7 cut(s) 38, 208, 1778, 1804, 1937, 1994, 2638
RsaNI GTAC 7 cut(s) 37, 207, 1777, 1803, 1936, 1993, 2637
RseI CAYNNNNRTG 6 cut(s) 597, 603, 735, 1784, 2040, 2987
SacI GAGCTC 1 cut(s) 1148
SaqAI TTAA 7 cut(s) 915, 1284, 1356, 1410, 2240, 2264, 2747
SatI GCNGC 7 cut(s) 1386, 1389, 1436, 1439, 1824, 2210, 3303
Sau96I GGNCC 7 cut(s) 429, 1058, 1916, 2642, 2950, 3134, 3313
ScaI AGTACT 1 cut(s) 1994
SchI GAGTC 2 cut(s) 257, 1387
ScrFI CCNGG 9 cut(s) 1321, 1748, 2172, 2448, 2529, 2646, 3038, 3493, 3589
SduI GDGCHC 5 cut(s) 281, 1148, 2404, 2857, 3379
SexAI ACCWGGT 1 cut(s) 1319
SfcI CTRYAG 4 cut(s) 1329, 1833, 2187, 3282
Sfr274I CTCGAG 1 cut(s) 32
SinI GGWCC 3 cut(s) 1916, 2950, 3313
SlaI CTCGAG 1 cut(s) 32
SmiMI CAYNNNNRTG 6 cut(s) 597, 603, 735, 1784, 2040, 2987
SmlI CTYRAG 2 cut(s) 32, 1271
SmoI CTYRAG 2 cut(s) 32, 1271
SpeI ACTAGT 1 cut(s) 2578
SphI GCATGC 1 cut(s) 2328
SseBI AGGCCT 1 cut(s) 3019
SsiI CCGC 7 cut(s) 580, 715, 2077, 2659, 2683, 3445, 3557
SspI AATATT 1 cut(s) 1353
SspMI CTAG 9 cut(s) 83, 104, 2570, 2579, 2858, 3021, 3306, 3338, 3485
SstI GAGCTC 1 cut(s) 1148
StuI AGGCCT 1 cut(s) 3019
StyD4I CCNGG 9 cut(s) 1319, 1746, 2170, 2446, 2527, 2644, 3036, 3491, 3587
StyI CCWWGG 9 cut(s) 45, 54, 243, 375, 1484, 2115, 2953, 3020, 3137
TaaI ACNGT 9 cut(s) 137, 179, 1173, 1600, 1927, 2248, 2846, 3457, 3505
TaiI ACGT 3 cut(s) 1816, 3237, 3550
TaqI TCGA 8 cut(s) 33, 481, 628, 1040, 2148, 2423, 2502, 3166
TatI WGTACW 3 cut(s) 1802, 1935, 1992
TfiI GAWTC 7 cut(s) 1019, 1360, 1457, 1477, 1966, 2573, 2992
Tru1I TTAA 7 cut(s) 915, 1284, 1356, 1410, 2240, 2264, 2747
Tru9I TTAA 7 cut(s) 915, 1284, 1356, 1410, 2240, 2264, 2747
TscAI CASTG 8 cut(s) 286, 1695, 1918, 2251, 2416, 2674, 2968, 3379
TseFI GTSAC 7 cut(s) 1063, 1338, 1600, 2101, 2279, 2331, 3451
TseI GCWGC 7 cut(s) 1385, 1388, 1435, 1438, 1823, 2209, 3302
Tsp45I GTSAC 7 cut(s) 1063, 1338, 1600, 2101, 2279, 2331, 3451
TspGWI ACGGA 4 cut(s) 194, 681, 1958, 2282
TspRI CASTG 8 cut(s) 286, 1695, 1918, 2251, 2416, 2674, 2968, 3379
Van91I CCANNNNNTGG 2 cut(s) 1508, 3418
VpaK11BI GGWCC 3 cut(s) 1916, 2950, 3313
XapI RAATTY 4 cut(s) 152, 2256, 2715, 3253
XceI RCATGY 2 cut(s) 1567, 2328
XcmI CCANNNNNNNNNTGG 1 cut(s) 3475
XhoI CTCGAG 1 cut(s) 32
XmaJI CCTAGG 1 cut(s) 3020
XmnI GAANNNNTTC 1 cut(s) 1680
XspI CTAG 9 cut(s) 83, 104, 2570, 2579, 2858, 3021, 3306, 3338, 3485
ZrmI AGTACT 1 cut(s) 1994
Zsp2I ATGCAT 1 cut(s) 2236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.